% EZvInitLoad global Exp global Expaa global Expbb global userPars global scansDir global matFile global zonePB global mpdmFile global ghandles % Initialize some variables from matlab ezPath=which(mfilename); [ezDir,ezFileName]=fileparts(ezPath); ezDir=fullfile(easyDir); [parentDir, ~]=fileparts(ezDir); parentDir=fullfile(parentDir); debug=1; if debug disp('Running in debug mode'); disp('Initialized variables:'); whos; end fprintf('This script name: %s\n', ezFileName); % Set scansDir (project scans directory) intelligently if exist('PROJECT_SCANS_DIR', 'var') && ~isempty(getenv('PROJECT_SCANS_DIR')) scansDir=fullfile(getenv('PROJECT_SCANS_DIR')); if exist(scansDir, 'dir') fprintf('Using scans directory: %s from environment variable PROJECT_SCANS_DIR\n', scansDir); disp('This usually indicates that we are in module mode'); if ~exist('PROJECT', 'var') || isempty(getenv('PROJECT')) dirInfo=fileparts(scansDir); project=dirInfo.name; end else disp('WARNING: PROJECT_SCANS_DIR does not exist'); end elseif exist('PROJECT', 'var') && ~isempty(getenv('PROJECT')) % for standalone mode % scansDir=fullfile(parentDir, '..', '..', 'scans', getenv('PROJECT') 'out', 'easy', getenv('PROJECT')); scansDir=fullfile(parentDir, '..', '..', 'scans', getenv('PROJECT')); project=getenv('PROJECT'); fprintf('Using project path: %s from environment variable PROJECT\n', scansDir); disp('This usually indicates that we are in standalone mode'); else % TODO Lots of this is hardcoded logic, this TODO is just a reminder to change this block % when changing EASY/EZdir and other variables in the parent script fprintf('WARNING: Running in standalone mode without PROJECT or PROJECT_SCANS_DIR environment variables (not recommended)\n'); fprintf('Beginning parent scans directory search\n'); dirsToScan={ fullfile(parentDir, '..', '..', 'scans') fullfile(parentDir, '..', '..', 'ExpJobs') fullfile('mnt','data','scans') fullfile('mnt','data', 'ExpJobs') fullfile(parentDir, '..', '..', 'templates', 'scans-demo') }; for i=1:length(dirsToScan) d=dirsToScan(i); d=char(d); if exist(d, 'dir') subDirs=dir(d); pattern='^\d{6}_.*_.*'; matchedDirs={dirs(regexp(subDirs.name, pattern)).name}; if ~isempty(matchedDirs) fprintf('Found a non-empty scans directory in our list: %s\n', char(d)); fprintf('Setting scansDir to %s\n', char(d)); scansDir=fullfile(d); end fprintf('Scanning inside %s for a project directory\n', char(d)); sortedMatchedDirs=sortrows(matchedDirs); project=sortedMatchedDirs{1}; % select the latest dir (by date prefix) fprintf('Selected latest project directory %s\n', char(project)); end end end % User sanity check and warning if exist('PROJECT_USER', 'var') && ~isempty(getenv('PROJECT_USER')) if ~equal(getenv('PROJECT_USER'), userName) disp("WARNING: PROJECT_USER does not match the current namespace"); end end % Allow module to override hardcoded default EZview directory if exist('EZVIEW_DIR','var') && ~isempty(getenv('EZVIEW_DIR')) EZVIEW_DIR=fullfile(getenv('EZVIEW_DIR')); if ~strcmp(ezDir, EZVIEW_DIR) % sanity check disp("WARNING: EZVIEW_DIR does not match this script's hardcoded EZview location"); disp("This is probably OK but if strange beahvior arises, we'll need to fix it in code"); ezDir=EZVIEW_DIR; end fprintf('Using EZview script directory: %s from environment variable EZVIEW_DIR\n', ezDir); else fprintf('Using EZview script directory: %s from hardcoded default\n', ezDir); end % Allow module to override hardcoded default EASY directory if exist('EASY_DIR','var') && ~isempty(getenv('EASY_DIR')) EASY_DIR=fullfile(getenv('EASY_DIR')); if ~strcmp(easyDir, EASY_DIR) % sanity check disp("WARNING: EASY_DIR does not match this script's hardcoded EASY location"); disp("This is probably OK but if strange beahvior arises, we'll need to fix it in code"); easyDir=EASY_DIR; end fprintf('Using EASY script directory: %s from environment variable EASY_DIR\n', easyDir); else fprintf('Using EASY script directory: %s from hardcoded default\n', easyDir); end userPars.BPdefault={'on','on','0.3','8','45','1000'}; userPars.boxplotFlg=1; userPars.BPoutliers='On'; userPars.BPnotch='On'; userPars.BPlblShft=0.3; userPars.BPfontSz=8; userPars.BProt=45; userPars.Trenddefault={'1000'}; userPars.kfiltLim=str2double(userPars.BPdefault(6)); try % Generate the Exp arrays numExps=3; for i=1:numExps Exp(i).cTraceN=0; Exp(i).cTraceIndx(1)=1; end Exp(1).CompositPlot=0;Exp(2).CompositPlot=0;Exp(3).CompositPlot=0; for i=1:numExps Exp(i).DexpType='single'; Exp(i).DexpN=1; Exp(i).DexpLength=1; eval(['set(handles.DN' num2str(i) ', ''value'', 1);']); % MATLAB level unlocked end set(handles.GeneOrfTog,'value',0) try load('EZsgdInfo'); catch disp('Fail to load EZsgdInfo file from code directory'); end Exp(numExps+1).SGDnum=SGDnum; Exp(numExps+1).SGDtext=SGDtext; Exp(numExps+1).SGDraw=SGDraw; Exp(numExps+1).interacPBsel=0; % Big outer loop try catch to ln844 end ln868 % try to use user selected 'ExpJobs' folder otherwise use a default % directory in the uigetfile to load the experiment .mat file. try [matFile,scansDir]=uigetfile('.mat','Open Experiment folder and data storage .mat file name','MultiSelect','off') load (fullfile(Exp(1).Dexp(1).ExpFoldr,'MasterPlateFiles','MPDMmat.mat')) for i=1:numExps Exp(i).Dexp(1).DM=DM; Exp(i).Dexp(1).MP=MP; Exp(i).Dexp(1).scan=scan; Exp(i).cTraceIndx(1)=1; end load(fullfile(resDir,'PTmats','NImParameters')) Exp(1).Dexp(1).ImParMat=ImParMat; Exp(2).Dexp(1).ImParMat=ImParMat; Exp(3).Dexp(1).ImParMat=ImParMat; try load(fullfile(resDir,'Fotos','Coordinates')) load(fullfile(resDir,'Fotos','anlZones')) load(fullfile(resDir,'Fotos','BGatTpts')) for i=1:numExps Exp(i).Dexp(1).FexpScanSpots=FexpScanSpots; Exp(i).Dexp(1).FexpScanBMtp=FexpScanBMtp; Exp(i).Dexp(1).anlZoneRefs=anlZoneRefs; Exp(i).cTraceIndx(1)=1; end catch end for i=1:numExps Exp(i).traceN=0; % ZoneRelated Exp(i).traceN=0; Exp(i).hOL=[]; Exp(i).hOLb=[]; Exp(i).hOLname={}; Exp(i).hOLexpNm={}; Exp(i).hOLresDir={} Exp(i).hOLplateNum=[]; Exp(i).hOLresDir={}; Exp(i).RFmd1indx=[]; Exp(i).RFmd1pltN=[]; Exp(i).RFmd2indx=[]; Exp(i).RFmd2pltN=[]; end % default values for EZvFigPrint that meet JLH preferences Exp(4).plotPars={'4','36','10','5'}; expN=1; % Test for Bad MP cell array (usually 384 [NaN}'s) % replaced length(Exp(expN).Dexp(1).MP) with MPnum for mx=1:length(Exp(expN).Dexp(1).MP) try char((Exp(1).Dexp(1).MP(mx).genename{1}(384))) MPnum=mx; catch break end end % MPnum=length(Exp(expN).Dexp(1).MP); set(handles.MPsldr1,'min',1,'max',MPnum) set(handles.MPsldr2,'min',1,'max',MPnum) set(handles.MPsldr3,'min',1,'max',MPnum) DMnum=length(Exp(expN).Dexp(1).DM.drug); set(handles.DMsldr1,'min',1,'max',DMnum) %DMnum,'value',1.01) %100,'value',1.01) set(handles.DMsldr2,'min',1,'max',DMnum) set(handles.DMsldr3,'min',1,'max',DMnum) tPtsSize=size(Exp(expN).Dexp(1).FexpScanBMtp{1,1},(3)); set(handles.Tptsldr1,'min',1,'max',tPtsSize) set(handles.Tptsldr2,'min',1,'max',tPtsSize) set(handles.Tptsldr3,'min',1,'max',tPtsSize) set(handles.MPsldr1,'SliderStep',[1/MPnum 1/MPnum]); %[1/length(MP) 1/length(MP)]) set(handles.DMsldr1,'SliderStep',[1/length(DM.drug) 1/length(DM.drug)]); set(handles.Tptsldr1,'SliderStep',[1/tPtsSize 1/tPtsSize]); set(handles.MPsldr2,'SliderStep',[1/MPnum 1/MPnum]); set(handles.DMsldr2,'SliderStep',[1/length(DM.drug) 1/length(DM.drug)]); set(handles.Tptsldr2,'SliderStep',[1/tPtsSize 1/tPtsSize]); set(handles.MPsldr3,'SliderStep',[1/MPnum 1/MPnum]); set(handles.DMsldr3,'SliderStep',[1/length(DM.drug) 1/length(DM.drug)]); set(handles.Tptsldr3,'SliderStep',[1/tPtsSize 1/tPtsSize]); try xp=char(Exp(expN).Dexp(1).resDir); if ispc slashPos=strfind(char(Exp(expN).Dexp(1).resDir),'\'); else slashPos=strfind(char(Exp(expN).Dexp(1).resDir),'/'); end startPos=slashPos(length(slashPos)-1) +1; endPos=slashPos(length(slashPos)) -1; expStr=xp(startPos:endPos); set(handles.expName1,'string',expStr); set(handles.expName2,'string',expStr); set(handles.expName3,'string',expStr); catch cd(w) end %internal try xp=char(Exp(expN).Dexp(1).resDir); if ispc,... catch cd(w) msg='LOADing or Setup issue' % if no load, then Message try SelExp PB to reload or try and different experiment end try DNLaxes1=handles.DNLaxes1; DNLaxes2=handles.DNLaxes2; DNLaxes3=handles.DNLaxes3; zeroCLn=zeros(1,DMnum); %Exp(expN).expLoadCnt(1)=1; %ZoneRelated Exp(1).hzeroCLn(1)=plot(DNLaxes1,zeroCLn,1:DMnum,'y'); try set(Exp(1).hzeroCLn,'visible','off'); catch ME, end Exp(2).hzeroCLn(1)=plot(DNLaxes2,zeroCLn,1:DMnum,'y'); try set(Exp(2).hzeroCLn,'visible','off'); catch ME, end Exp(3).hzeroCLn(1)=plot(DNLaxes3,zeroCLn,1:DMnum,'y'); try set(Exp(3).hzeroCLn,'visible','off'); catch ME, end Exp(1).expLoadCnt=1; Exp(2).expLoadCnt=1; Exp(3).expLoadCnt=1; catch ME ME.message, end % Compile GeneList and OrfList % Sort and add the medians of Ref Plates if RF1,RF2 exist set(handles.listboxGnOrf,'string',{('Loading')}) lstindx=0; lstindxOrf=0; spN=0; rfcnt=0; dRF1indx=0; RF1mp=[]; RF2mp=[]; for mp=1:MPnum %length(Exp(expN).Dexp(1).MP) RF1fullPlate=0; try RF1fullPlate=sum(ismember((Exp(expN).Dexp(1).MP(mp).genename{1}),'RF1'))==384; catch, mp, msg=strcat(num2str(mp),' genename ERROR'), end RF2fullPlate=0; try RF2fullPlate=sum(ismember((Exp(expN).Dexp(1).MP(mp).genename{1}),'RF2'))==384; catch, mp, msg=strcat(num2str(mp),' genename ERROR'), end for ind384=1:384 spN=spN+1; try % temp find data error r=ceil(ind384/24); if rem(ind384,24)==0, c=24; else c=rem(ind384,24); end % Insert test for numeric in genename and orf if isnumeric % Correct common EXCEL problem of converting OCT1 into a date numeric if cell2mat(Exp(expN).Dexp(1).MP(mp).genename{1}(ind384))==38991, Exp(expN).Dexp(1).MP(mp).genename{1}(ind384)={'OCT1_'}; % genename{1}(ind384) replace numeric with a space ' ' or something elseif isnumeric(cell2mat(Exp(expN).Dexp(1).MP(mp).genename{1}(ind384))) Exp(expN).Dexp(1).MP(mp).genename{1}(ind384)={' '}; end % Check for full(384spot) Reference Plates (RF1 or/and RF2) RF1fullPlate=0; try RF1fullPlate=sum(ismember((Exp(expN).Dexp(1).MP(mp).genename{1}),'RF1'))==384; catch, mp, msg=strcat(num2str(mp),' genename ERROR'), end RF2fullPlate=0; try RF2fullPlate=sum(ismember((Exp(expN).Dexp(1).MP(mp).genename{1}),'RF2'))==384; catch, mp, msg=strcat(num2str(mp),' genename ERROR'), end if isempty(regexpi(char((Exp(expN).Dexp(1).MP(mp).orf{1}(ind384))),'blank'))... %,'\' && ~isnumeric(Exp(expN).Dexp(1).MP(mp).orf{1}(ind384))... && ~strcmpi((Exp(expN).Dexp(1).MP(mp).orf{1}(ind384)),' ')... && ~strcmpi((Exp(expN).Dexp(1).MP(mp).orf{1}(ind384)),' ')... && ~strcmpi((Exp(expN).Dexp(1).MP(mp).orf{1}(ind384)),'')... && ~isempty((Exp(expN).Dexp(1).MP(mp).orf{1}(ind384)))... && ~strcmpi((Exp(expN).Dexp(1).MP(mp).orf{1}(ind384)),'RF1')... && ~strcmpi((Exp(expN).Dexp(1).MP(mp).orf{1}(ind384)),'RF2')... && iscellstr((Exp(expN).Dexp(1).MP(mp).orf{1}(ind384))) % .genename{1}(ind384))) lstindxOrf=lstindxOrf+1; bb(lstindxOrf,1)=(Exp(expN).Dexp(1).MP(mp).orf{1}(ind384)); bb(lstindxOrf,2)={strcat(':',num2str(mp),':',num2str(r),':',num2str(c))}; bb(lstindxOrf,3)={mp}; bb(lstindxOrf,4)={r}; bb(lstindxOrf,5)={c}; mutorfs(lstindxOrf)=spN; %16_0318 added for Interaction EZinteract end if isempty(regexpi(char((Exp(expN).Dexp(1).MP(mp).genename{1}(ind384))),'blank'))... %,'\' && ~isnumeric(Exp(expN).Dexp(1).MP(mp).genename{1}(ind384))... && ~strcmpi((Exp(expN).Dexp(1).MP(mp).genename{1}(ind384)),' ')... && ~strcmpi((Exp(expN).Dexp(1).MP(mp).genename{1}(ind384)),' ')... && ~strcmpi((Exp(expN).Dexp(1).MP(mp).genename{1}(ind384)),'')... && ~isempty((Exp(expN).Dexp(1).MP(mp).genename{1}(ind384)))... && ~strcmpi((Exp(expN).Dexp(1).MP(mp).genename{1}(ind384)),'RF1')... && ~strcmpi((Exp(expN).Dexp(1).MP(mp).genename{1}(ind384)),'RF2')... && iscellstr((Exp(expN).Dexp(1).MP(mp).genename{1}(ind384))) lstindx=lstindx+1; aa(lstindx,1)=(Exp(expN).Dexp(1).MP(mp).genename{1}(ind384)); aa(lstindx,2)={strcat(':',num2str(mp),':',num2str(r),':',num2str(c))}; aa(lstindx,3)={mp}; aa(lstindx,4)={r}; aa(lstindx,5)={c}; mutgenes(lstindx)=spN; % Exp(expN).Dexp(1).mutSpotIndx.woRFs(lstindx)=spN; elseif (RF1fullPlate==1 ... % sum(ismember((Exp(expN).Dexp(1).MP(mp).genename{1}),'RF1'))==384 || ... || (length(unique(Exp(expN).Dexp(1).MP(mp).genename{1}(1:384))))==1 && mp==1 ) ... %(isequal((Exp(expN).Dexp(1).MP(mp).genename{1}(1:384)), (Exp(expN).Dexp(1).MP(mp).genename{1}(384:-1:1)))&& mp==1 )) && ... && ~exist('RF1scanN','var') RF1mp=mp RF1scanN=(mp*DMnum)-(DMnum-1); %mp; rfcnt=rfcnt+1; RFs(spN:spN+383)=spN:spN+383; elseif (RF2fullPlate==1 ... %sum(ismember((Exp(expN).Dexp(1).MP(mp).genename{1}(ind384)),'RF2'))==384 ||... || (length(unique(Exp(expN).Dexp(1).MP(mp).genename{1}(1:384))))==1 && mp==MPnum ) && ~exist('RF2scanN','var') rfcnt=rfcnt+1; RFs(spN:spN+383)=spN:spN+383; asdf=sparse(RFs); asd=find(asdf); RFs=asd; RF2mp=mp RF2scanN=(mp*DMnum)-(DMnum-1); %mp; elseif strcmpi((Exp(expN).Dexp(1).MP(mp).genename{1}(ind384)),'RF1') && (RF1fullPlate~=1) dRF1indx=dRF1indx+1; Exp(expN).Dexp(1).MP(mp).genename{1}(ind384)=strrep((Exp(expN).Dexp(1).MP(mp).genename{1}(ind384)),':',' '); drf(dRF1indx,1)=(Exp(expN).Dexp(1).MP(mp).genename{1}(ind384)); drf(dRF1indx,2)={strcat(':',num2str(mp),':',num2str(r),':',num2str(c))}; drf(dRF1indx,3)={mp}; drfMP(dRF1indx,mp)=mp; drf(dRF1indx,4)={r}; drfr(dRF1indx,mp)=r; drf(dRF1indx,5)={c}; drfc(dRF1indx,mp)=c; drf(dRF1indx,6)={ind384}; drfPindx(dRF1indx,mp)=ind384; end % if isempty(regexpi(char((Exp(expN).Dexp(1).MP(mp).genename{1}(ind384))),'blank'))... catch mp ind384 msgBadGeneName=strcat('check genename at mp=',num2str(mp),' indx=',num2str(ind384)) end end mp end % Cover case if mutorfs RFs are labeled RF1 or RF2 try Exp(1).Dexp(1).mutSpotIndx.wRFs=union(RFs, mutorfs); catch msg='No RFs Found in Exp! wRFs' Exp(1).Dexp(1).mutSpotIndx.wRFs=mutorfs; end try Exp(1).Dexp(1).mutSpotIndx.woRFs=setdiff(mutorfs,RFs); catch Exp(expN).Dexp(1).mutSpotIndx.woRFs=mutorfs; msg='No RFs Found in Exp! woRFs' end Exp(2).Dexp(1).mutSpotIndx.wRFs=Exp(1).Dexp(1).mutSpotIndx.wRFs; Exp(2).Dexp(1).mutSpotIndx.woRFs=Exp(1).Dexp(1).mutSpotIndx.woRFs; Exp(3).Dexp(1).mutSpotIndx.wRFs=Exp(1).Dexp(1).mutSpotIndx.wRFs; Exp(3).Dexp(1).mutSpotIndx.woRFs=Exp(1).Dexp(1).mutSpotIndx.woRFs; Exp(1).Dexp(1).RFmd1indx=[]; Exp(2).Dexp(1).RFmd1indx=[]; Exp(3).Dexp(1).RFmd1indx=[]; Exp(1).Dexp(1).RFmd1pltN=[]; Exp(2).Dexp(1).RFmd1pltN=[]; Exp(3).Dexp(1).RFmd1pltN=[]; Exp(1).Dexp(1).RFmean=[]; Exp(2).Dexp(1).RFmean=[]; Exp(3).Dexp(1).RFmean=[]; Exp(1).Dexp(1).RFmeanG=[]; Exp(2).Dexp(1).RFmeanG=[]; Exp(3).Dexp(1).RFmeanG=[]; if exist('RF1scanN','var') for d=0:(DMnum-1) medianIndx=[]; nonZeroIndx=[]; medValList=[]; rf1scN=RF1scanN+d; try %if RF1scanN doesn't exist this addition to list will be skipped nonZeroIndx=find(Exp(expN).Dexp(1).scan(rf1scN).plate(1).CFout(1:384,5)); if isempty(nonZeroIndx) nonZeroCnt=0 medianIndx=nonZeroIndx; else nonZeroCnt=length(nonZeroIndx); end if ~isempty(nonZeroIndx) && mod(nonZeroCnt,2)==0, medianIndx=nonZeroIndx(1:nonZeroCnt-1); end if ~isempty(nonZeroIndx) && mod(nonZeroCnt,2)~=0, medianIndx=nonZeroIndx; end clear medValLst clear kValLst clear rValLst if ~isempty(nonZeroIndx) && length(medianIndx) > (.15*384) % To calc. a median, more than 15% of spots must be nonZero medValLst=Exp(expN).Dexp(1).scan(rf1scN).plate(1).CFout(medianIndx,5); medValList1{d+1}=medValLst; min1=min(medValLst); max1=max(medValLst); std1=std(medValLst); mean1=mean(medValLst) kValLst=Exp(expN).Dexp(1).scan(rf1scN).plate(1).CFout(medianIndx,3); % For Ref Composite 17_1009 rValLst=Exp(expN).Dexp(1).scan(rf1scN).plate(1).CFout(medianIndx,4); % For Ref Composite 17_1009 mval1=median(medValLst); % (Exp(expN).Dexp(1).scan(RF1scanN).plate(1).CFout(383,5)); RFmd1pos=find(mval1==(Exp(expN).Dexp(1).scan(rf1scN).plate(1).CFout(1:384,5))) RFmd1loc(1)=ceil(RFmd1pos/24); %calc r value RFmd1loc(2)=RFmd1pos - ((RFmd1loc(1)-1)*24); %calc c value lstindx=lstindx+1; lstindxOrf=lstindxOrf+1; aa(lstindx,1)={strcat('RF1md(',num2str(d+1),')')}; aa(lstindx,2)={strcat(':',num2str(RF1mp),':',num2str(RFmd1loc(1)),':',num2str(RFmd1loc(2)))}; %,' std',num2str(std1))}; bb(lstindxOrf,1)={strcat('RF1md(',num2str(d+1),')')}; bb(lstindxOrf,2)={strcat(':',num2str(RF1mp),':',num2str(RFmd1loc(1)),':',num2str(RFmd1loc(2)))}; %,' std',num2str(std1))}; % Composite Reference Amalgum Plot lstindx=lstindx+1; lstindxOrf=lstindxOrf+1; aa(lstindx,1)={strcat('RF1cmp(',num2str(d+1),')')}; aa(lstindx,2)={''}; bb(lstindxOrf,1)={strcat('RF1cmp(',num2str(d+1),')')}; bb(lstindxOrf,2)={''}; Exp(1).Dexp(1).RFmd1LB(d+1)=strcat(aa(lstindx,1),aa(lstindx,2)); Exp(2).Dexp(1).RFmd1LB(d+1)=strcat(aa(lstindx,1),aa(lstindx,2)); Exp(3).Dexp(1).RFmd1LB(d+1)=strcat(aa(lstindx,1),aa(lstindx,2)); Exp(1).Dexp(1).RFmd1indx(d+1)=RFmd1pos; Exp(2).Dexp(1).RFmd1indx(d+1)=RFmd1pos; Exp(3).Dexp(1).RFmd1indx(d+1)=RFmd1pos; Exp(1).Dexp(1).RFmd1pltN=RF1mp; Exp(2).Dexp(1).RFmd1pltN=RF1mp; Exp(3).Dexp(1).RFmd1pltN=RF1mp; Exp(1).Dexp(1).RFmd1val(d+1)=mval1; Exp(2).Dexp(1).RFmd1val(d+1)=mval1; Exp(3).Dexp(1).RFmd1val(d+1)=mval1; Exp(1).Dexp(1).mean1(d+1)=mean1; Exp(2).Dexp(1).mean1(d+1)=mean1; Exp(3).Dexp(1).mean1(d+1)=mean1; Exp(1).Dexp(1).std1(d+1)=std1; Exp(2).Dexp(1).std1(d+1)=std1; Exp(3).Dexp(1).std1(d+1)=std1; Exp(1).Dexp(1).min1(d+1)=min1; Exp(2).Dexp(1).min1(d+1)=min1; Exp(3).Dexp(1).min1(d+1)=min1; Exp(1).Dexp(1).max1(d+1)=max1; Exp(2).Dexp(1).max1(d+1)=max1; Exp(3).Dexp(1).max1(d+1)=max1; for ic=1:3 Exp(ic).Dexp(1).RFcmpL(1).dm(d+1).Lvals=medValLst; %17_1201 TrendOL Exp(ic).Dexp(1).RFcmpL(1).dm(d+1).med=median(medValLst); %For Ref Composite 17_1009 Exp(ic).Dexp(1).RFcmpL(1).dm(d+1).mean=mean(medValLst); %For Ref Composite 17_1009 Exp(ic).Dexp(1).RFcmpL(1).dm(d+1).std=std(medValLst); %For Ref Composite 17_1009 Exp(ic).Dexp(1).RFcmpL(1).dm(d+1).min=min(medValLst); %For Ref Composite 17_1009 Exp(ic).Dexp(1).RFcmpL(1).dm(d+1).max=max(medValLst); %For Ref Composite 17_1009 Exp(ic).Dexp(1).RFcmpK(1).dm(d+1).Kvals=kValLst; %17_1201 TrendOL Exp(ic).Dexp(1).RFcmpK(1).dm(d+1).mean=mean(kValLst); %For Ref Composite 17_1009 Exp(ic).Dexp(1).RFcmpK(1).dm(d+1).med=median(kValLst); %For Ref Composite 17_1009 Exp(ic).Dexp(1).RFcmpK(1).dm(d+1).std=std(kValLst); %For Ref Composite 17_1009 Exp(ic).Dexp(1).RFcmpK(1).dm(d+1).min=min(kValLst); %For Ref Composite 17_1009 Exp(ic).Dexp(1).RFcmpK(1).dm(d+1).max=max(kValLst); %For Ref Composite 17_1009 Exp(ic).Dexp(1).RFcmpr(1).dm(d+1).rvals=rValLst; %17_1201 TrendOL Exp(ic).Dexp(1).RFcmpr(1).dm(d+1).med=median(rValLst); %For Ref Composite 17_1009 Exp(ic).Dexp(1).RFcmpr(1).dm(d+1).mean=mean(rValLst); %For Ref Composite 17_1009 Exp(ic).Dexp(1).RFcmpr(1).dm(d+1).std=std(rValLst); %For Ref Composite 17_1009 Exp(ic).Dexp(1).RFcmpr(1).dm(d+1).min=min(rValLst); %For Ref Composite 17_1009 Exp(ic).Dexp(1).RFcmpr(1).dm(d+1).mean=max(rValLst); %For Ref Composite 17_1009 end else % Pick the first spot with zeros medianCalcFailed='To Few nonZero spots for valid median RF1 selection' firstZero=find(((Exp(expN).Dexp(1).scan(rf1scN).plate(1).CFout(1:384,5))==0),1,'first') Exp(1).Dexp(1).RFmd1indx(d+1)=firstZero; Exp(2).Dexp(1).RFmd1indx(d+1)=firstZero; Exp(3).Dexp(1).RFmd1indx(d+1)=firstZero; Exp(1).Dexp(1).RFmd1pltN=RF1mp; Exp(2).Dexp(1).RFmd1pltN=RF1mp; Exp(3).Dexp(1).RFmd1pltN=RF1mp; %EZdestComp uses the masterplate number to get all the destPlates scanNumbers try, medValList1{d+1}=Exp(expN).Dexp(1).scan(rf1scN).plate(1).CFout(medianIndx,5); nonZeroCnt; catch end end catch medianCalcTryFailed1='Failed! Failed! But process continued with bogus P1ind384 spot value!' try firstZero=find(((Exp(expN).Dexp(1).scan(rf1scN).plate(1).CFout(1:384,5))==0),1,'first') Exp(1).Dexp(1).RFmd1indx(d+1)=firstZero; Exp(2).Dexp(1).RFmd1indx(d+1)=firstZero; Exp(3).Dexp(1).RFmd1indx(d+1)=firstZero; catch Exp(1).Dexp(1).RFmd1indx(d+1)=384; Exp(2).Dexp(1).RFmd1indx(d+1)=384; Exp(3).Dexp(1).RFmd1indx(d+1)=384; %if crapout,Then use spot384 as default to keep going end try Exp(1).Dexp(1).RFmd1pltN=RF1mp; Exp(2).Dexp(1).RFmd1pltN=RF1mp; Exp(3).Dexp(1).RFmd1pltN=RF1mp; catch catchissue='Ln334' Exp(1).Dexp(1).RFmd1pltN=1; Exp(2).Dexp(1).RFmd1pltN=1; Exp(3).Dexp(1).RFmd1pltN=1; end end end end Exp(1).Dexp(1).RFmd2indx=[]; Exp(2).Dexp(1).RFmd2indx=[]; Exp(3).Dexp(1).RFmd2indx=[]; Exp(1).Dexp(1).RFmd2pltN=[]; Exp(2).Dexp(1).RFmd2pltN=[]; Exp(3).Dexp(1).RFmd2pltN=[]; if exist('RF2scanN','var') for d=0:(DMnum-1); medianIndx2=[]; nonZeroIndx2=[]; medValList2=[]; rf2scN=RF2scanN+d; try % if RF2scanN doesn't exist this addition to list will be skipped nonZeroIndx2=find(Exp(expN).Dexp(1).scan(rf2scN).plate(1).CFout(1:384,5)); if isempty(nonZeroIndx2) nonZeroCnt2=0 medianIndx2=nonZeroIndx2; else nonZeroCnt2=length(nonZeroIndx2); end if ~isempty(nonZeroIndx2) && mod(nonZeroCnt2,2)==0, medianIndx2=nonZeroIndx2(1:nonZeroCnt2-1); end if ~isempty(nonZeroIndx2) && mod(nonZeroCnt2,2)~=0, medianIndx2=nonZeroIndx2; end clear medValLst2 clear kValLst2 clear rValLst2 if ~isempty(nonZeroIndx2) && length(medianIndx2) > (.15*384) % to calc. a median, more than 15% of spots must be nonZero medValLst2=Exp(expN).Dexp(1).scan(rf2scN).plate(1).CFout(medianIndx2,5); medValList2{d+1}=medValLst2; min2=min(medValLst2); max2=max(medValLst2); std2=std(medValLst2) mean2=mean(medValLst2); kValLst2=Exp(expN).Dexp(1).scan(rf2scN).plate(1).CFout(medianIndx2,3); %For Ref Composite 17_1009 rValLst2=Exp(expN).Dexp(1).scan(rf2scN).plate(1).CFout(medianIndx2,4); %For Ref Composite 17_1009 mval2=median(medValLst2); %(Exp(expN).Dexp(1).scan(RF2scanN).plate(1).CFout(1:383,5)); RFmd2pos=find(mval2==(Exp(expN).Dexp(1).scan(rf2scN).plate(1).CFout(1:384,5))); RFmd2loc(1)=ceil(RFmd2pos/24); %calc r value RFmd2loc(2)=RFmd2pos - ((RFmd2loc(1)-1)*24); %calc c value lstindx=lstindx+1; lstindxOrf=lstindxOrf+1; aa(lstindx,1)={strcat('RF2md(',num2str(d+1),')')}; %{'RF2md'}; aa(lstindx,2)={strcat(':',num2str(RF2mp),':',num2str(RFmd2loc(1)),':',num2str(RFmd2loc(2)))}; %,' std~',num2str(std2))}; bb(lstindxOrf,1)={strcat('RF2md(',num2str(d+1),')')}; %{'RF2md'}; bb(lstindxOrf,2)={strcat(':',num2str(RF2mp),':',num2str(RFmd2loc(1)),':',num2str(RFmd2loc(2)))}; %,' std~',num2str(std2))}; %Composite Reference Amalgum Plot (CRAP) lstindx=lstindx+1; lstindxOrf=lstindxOrf+1; aa(lstindx,1)={strcat('RF2cmp(',num2str(d+1),')')}; aa(lstindx,2)={''}; bb(lstindxOrf,1)={strcat('RF2cmp(',num2str(d+1),')')}; bb(lstindxOrf,2)={''}; Exp(1).Dexp(1).RFmd2LB(d+1)=strcat(aa(lstindx,1),aa(lstindx,2)); Exp(2).Dexp(1).RFmd2LB(d+1)=strcat(aa(lstindx,1),aa(lstindx,2)); Exp(3).Dexp(1).RFmd2LB(d+1)=strcat(aa(lstindx,1),aa(lstindx,2)); Exp(1).Dexp(1).RFmd2indx(d+1)=RFmd2pos; Exp(2).Dexp(1).RFmd2indx(d+1)=RFmd2pos; Exp(3).Dexp(1).RFmd2indx(d+1)=RFmd2pos; Exp(1).Dexp(1).RFmd2pltN=RF2mp; Exp(2).Dexp(1).RFmd2pltN=RF2mp; Exp(3).Dexp(1).RFmd2pltN=RF2mp; Exp(1).Dexp(1).RFmd2val(d+1)=mval2; Exp(2).Dexp(1).RFmd2val(d+1)=mval2; Exp(3).Dexp(1).RFmd2val(d+1)=mval2; Exp(1).Dexp(1).mean2(d+1)=mean2; Exp(2).Dexp(1).mean2(d+1)=mean2; Exp(3).Dexp(1).mean2(d+1)=mean2; Exp(1).Dexp(1).std2(d+1)=std2; Exp(2).Dexp(1).std2(d+1)=std2; Exp(3).Dexp(1).std2(d+1)=std2; Exp(1).Dexp(1).min2(d+1)=min2; Exp(2).Dexp(1).min2(d+1)=min2; Exp(3).Dexp(1).min2(d+1)=min2; Exp(1).Dexp(1).max2(d+1)=max2; Exp(2).Dexp(1).max2(d+1)=max2; Exp(3).Dexp(1).max2(d+1)=max2; for ic=1:3 Exp(ic).Dexp(1).RFcmpL(2).dm(d+1).Lvals=medValLst2; %17_1201 TrendOL Exp(ic).Dexp(1).RFcmpL(2).dm(d+1).med=median(medValLst2); %For Ref Composite 17_1009 Exp(ic).Dexp(1).RFcmpL(2).dm(d+1).mean=mean(medValLst2); %For Ref Composite 17_1009 Exp(ic).Dexp(1).RFcmpL(2).dm(d+1).std=std(medValLst2); %For Ref Composite 17_1009 Exp(ic).Dexp(1).RFcmpL(2).dm(d+1).min=min(medValLst2); %For Ref Composite 17_1009 Exp(ic).Dexp(1).RFcmpL(2).dm(d+1).max=max(medValLst2); %For Ref Composite 17_1009 Exp(ic).Dexp(1).RFcmpK(2).dm(d+1).Kvals=kValLst2; %17_1201 TrendOL Exp(ic).Dexp(1).RFcmpK(2).dm(d+1).med=median(kValLst2); %For Ref Composite 17_1009 Exp(ic).Dexp(1).RFcmpK(2).dm(d+1).mean=mean(kValLst2); %For Ref Composite 17_1009 Exp(ic).Dexp(1).RFcmpK(2).dm(d+1).std=std(kValLst2); %For Ref Composite 17_1009 Exp(ic).Dexp(1).RFcmpK(2).dm(d+1).min=min(kValLst2); %For Ref Composite 17_1009 Exp(ic).Dexp(1).RFcmpK(2).dm(d+1).max=max(kValLst2); %For Ref Composite 17_1009 Exp(ic).Dexp(1).RFcmpr(2).dm(d+1).rvals=rValLst2; %17_1201 TrendOL Exp(ic).Dexp(1).RFcmpr(2).dm(d+1).med=median(rValLst2); %For Ref Composite 17_1009 Exp(ic).Dexp(1).RFcmpr(2).dm(d+1).mean=mean(rValLst2); %For Ref Composite 17_1009 Exp(ic).Dexp(1).RFcmpr(2).dm(d+1).std=std(rValLst2); %For Ref Composite 17_1009 Exp(ic).Dexp(1).RFcmpr(2).dm(d+1).min=min(rValLst2); %For Ref Composite 17_1009 Exp(ic).Dexp(1).RFcmpr(2).dm(d+1).max=max(rValLst); %For Ref Composite 17_1009 end else % Pick the first spot with zeros % Use that position to satisfy and keep process OK medianCalcFailed2='To Few nonZero spots for valid median RF2 selection' firstZero2=find(((Exp(expN).Dexp(1).scan(rf2scN).plate(1).CFout(1:384,5))==0),1,'first') Exp(1).Dexp(1).RFmd2indx(d+1)=firstZero2; Exp(2).Dexp(1).RFmd2indx(d+1)=firstZero2; Exp(3).Dexp(1).RFmd2indx(d+1)=firstZero2; Exp(1).Dexp(1).RFmd2pltN=RF2mp; Exp(2).Dexp(1).RFmd2pltN=RF2mp; Exp(3).Dexp(1).RFmd2pltN=RF2mp; try medValList2{d+1}=Exp(expN).Dexp(1).scan(rf2scN).plate(1).CFout(medianIndx2,5); nonZeroCnt2; catch end end catch medianCalcTryFailed2='Failed! Failed! No RF1median But process continued with bogus P1ind384 spot value!' try firstZero2=find(((Exp(expN).Dexp(1).scan(rf2scN).plate(1).CFout(1:384,5))==0),1,'first') Exp(1).Dexp(1).RFmd2indx(d+1)=firstZero2; Exp(2).Dexp(1).RFmd2indx(d+1)=firstZero2; Exp(3).Dexp(1).RFmd2indx(d+1)=firstZero2; catch Exp(1).Dexp(1).RFmd2indx(d+1)=384; Exp(2).Dexp(1).RFmd2indx(d+1)=384; Exp(3).Dexp(1).RFmd2indx(d+1)=384; %if crapout,Then use spot384 as default to keep going end try Exp(1).Dexp(1).RFmd2pltN=RF2mp; Exp(2).Dexp(1).RFmd2pltN=RF2mp; Exp(3).Dexp(1).RFmd2pltN=RF2mp; catch Exp(1).Dexp(1).RFmd2pltN=1; Exp(2).Dexp(1).RFmd2pltN=1; Exp(3).Dexp(1).RFmd2pltN=1; end end % if RF2scanN doesn't exist this addition to list will be skipped RFcombValList=cat(1,medValList1{d+1},medValList2{d+1}); Exp(1).Dexp(1).RFmean(d+1)=mean(RFcombValList);Exp(2).Dexp(1).RFmean(d+1)=mean(RFcombValList);Exp(3).Dexp(1).RFmean(d+1)=mean(RFcombValList); Exp(1).Dexp(1).RFstd(d+1)=std(RFcombValList);Exp(2).Dexp(1).RFstd(d+1)=std(RFcombValList);Exp(3).Dexp(1).RFstd(d+1)=std(RFcombValList); Exp(1).Dexp(1).RFmin(d+1)=min(RFcombValList);Exp(2).Dexp(1).RFmin(d+1)=min(RFcombValList);Exp(3).Dexp(1).RFmin(d+1)=min(RFcombValList); Exp(1).Dexp(1).RFmax(d+1)=max(RFcombValList);Exp(2).Dexp(1).RFmax(d+1)=max(RFcombValList);Exp(3).Dexp(1).RFmax(d+1)=max(RFcombValList); % TODO can delete the following redundant line of code Exp(2).Dexp(1).RFmean(d+1)=Exp(1).Dexp(1).RFmean(d+1); Exp(3).Dexp(1).RFmean(d+1)=Exp(1).Dexp(1).RFmean(d+1); end elseif exist('RF1scanN','var') Exp(1).Dexp(1).RFmean=Exp(1).Dexp(1).mean1; Exp(2).Dexp(1).RFmean=Exp(2).Dexp(1).mean1; Exp(3).Dexp(1).RFmean=Exp(3).Dexp(1).mean1; Exp(1).Dexp(1).RFstd=Exp(1).Dexp(1).std1; Exp(2).Dexp(1).RFstd=Exp(2).Dexp(1).std1; Exp(3).Dexp(1).RFstd=Exp(3).Dexp(1).std1; Exp(1).Dexp(1).RFmin=Exp(1).Dexp(1).min1; Exp(2).Dexp(1).RFmin=Exp(2).Dexp(1).min1; Exp(3).Dexp(1).RFmin=Exp(3).Dexp(1).min1; Exp(1).Dexp(1).RFmax=Exp(1).Dexp(1).max1; Exp(2).Dexp(1).RFmax=Exp(2).Dexp(1).max1; Exp(3).Dexp(1).RFmax=Exp(3).Dexp(1).max1; end % DISPERSE REFERENCE PLATES Exp(expN).Dexp(1).RFmdPindx=[]; Exp(expN).Dexp(1).RFmdPpltN=[]; if exist('drf','var') % ('RF1scanN','var') % Det. index of change from one MP to the next i=2; chgIndx(1)=1; for j=1:length(drf(:,3)) if j>1 if cell2mat(drf(j,3))~=cell2mat(drf((j-1),3)), chgIndx(i)=j; i=i+1; end end end chgIndx(length(chgIndx)+1)=length(drf)+1; uu=zeros(size(drf,1),4); uu(:,1:4)=cell2mat(drf(:,3:6)); vv=zeros(length(drf),DMnum); dMPs=unique(cell2mat(drf(:,3))); for mm=1:length(dMPs) for d=1:(DMnum) medianIndxDisp=[]; NZrefIndxP=[]; nonZeroValsP=[]; medValLstDisp=[]; rf1scNdisp=((dMPs(mm)-1)*DMnum)+(d); try % Determine the dispersed RFs median mean and std if such exist NZrefIndxP=drfPindx((find(drfPindx(:,dMPs(mm)))),dMPs(mm)); vv(chgIndx(mm):(chgIndx(mm+1)-1),d)=Exp(expN).Dexp(1).scan(rf1scNdisp).plate(1).CFout(NZrefIndxP,5); % For Global Ref Composite 17_1009 vvK(chgIndx(mm):(chgIndx(mm+1)-1),d)=Exp(expN).Dexp(1).scan(rf1scNdisp).plate(1).CFout(NZrefIndxP,3); vvr(chgIndx(mm):(chgIndx(mm+1)-1),d)=Exp(expN).Dexp(1).scan(rf1scNdisp).plate(1).CFout(NZrefIndxP,4); nonZeroValsP=nonzeros(Exp(expN).Dexp(1).scan(rf1scNdisp).plate(1).CFout(NZrefIndxP,5)); minDisp=min(nonZeroValsP); maxDisp=max(nonZeroValsP); std1Disp=std(nonZeroValsP); mean1Disp=mean(nonZeroValsP); drfVals=Exp(expN).Dexp(1).scan(rf1scNdisp).plate(1).CFout(NZrefIndxP,5); %MEDIAN Determination for PLATES with Dispersed RFs if isempty(NZrefIndxP) nonZeroCntDisp=0 medianIndxDisp=NZrefIndxP; else nonZeroCntDisp=length(NZrefIndxP); end if ~isempty(NZrefIndxP) && mod(nonZeroCntDisp,2)==0, medianIndxDisp=NZrefIndxP(1:nonZeroCntDisp-1); end if ~isempty(NZrefIndxP) && mod(nonZeroCntDisp,2)~=0, medianIndxDisp=NZrefIndxP; end if ~isempty(NZrefIndxP) && length(medianIndxDisp) > 3 %(.15*384) %To calc. a median, more than 15% of spots must be nonZero medValLstDisp=Exp(expN).Dexp(1).scan(rf1scNdisp).plate(1).CFout(medianIndxDisp,5); mval1Disp=median(medValLstDisp); nonZeroMedPosIndx=find(mval1Disp==nonZeroValsP); RFmd1posD=NZrefIndxP(nonZeroMedPosIndx); RFmd1locD(1)=ceil(RFmd1posD/24); %calc r value RFmd1locD(2)=RFmd1posD - ((RFmd1locD(1)-1)*24); %calc c value lstindx=lstindx+1; lstindxOrf=lstindxOrf+1; aa(lstindx,1)={strcat('RF1mdP(',num2str(d),')')}; %{'RF1md'}; aa(lstindx,2)={strcat(':',num2str(dMPs(mm)),':',num2str(RFmd1locD(1)),':',num2str(RFmd1locD(2)))}; %,'std_',num2str(std1))}; bb(lstindxOrf,1)={strcat('RF1mdP(',num2str(d),')')}; %{'RF1md'}; bb(lstindxOrf,2)={strcat(':',num2str(dMPs(mm)),':',num2str(RFmd1locD(1)),':',num2str(RFmd1locD(2)))}; %,'std_',num2str(std1))}; Exp(1).Dexp(1).RFmdPLB(d,dMPs(mm))=strcat(aa(lstindx,1),aa(lstindx,2)); Exp(2).Dexp(1).RFmdPLB(d,dMPs(mm))=strcat(aa(lstindx,1),aa(lstindx,2)); Exp(3).Dexp(1).RFmdPLB(d,dMPs(mm))=strcat(aa(lstindx,1),aa(lstindx,2)); Exp(1).Dexp(1).RFmdPindx(d,dMPs(mm))=RFmd1posD; Exp(2).Dexp(1).RFmdPindx(d,dMPs(mm))=RFmd1posD; Exp(3).Dexp(1).RFmdPindx(d,dMPs(mm))=RFmd1posD; Exp(1).Dexp(1).RFmdPpltN(d,dMPs(mm))=dMPs(mm); Exp(2).Dexp(1).RFmdPpltN(d,dMPs(mm))=dMPs(mm); Exp(3).Dexp(1).RFmdPpltN(d,dMPs(mm))=dMPs(mm); Exp(1).Dexp(1).RFmedianP(d,dMPs(mm))=mval1Disp; Exp(2).Dexp(1).RFmedianP(d,dMPs(mm))=mval1Disp; Exp(3).Dexp(1).RFmedianP(d,dMPs(mm))=mval1Disp; Exp(1).Dexp(1).meanP(d,dMPs(mm))=mean1Disp; Exp(2).Dexp(1).meanP(d,dMPs(mm))=mean1Disp; Exp(3).Dexp(1).meanP(d,dMPs(mm))=mean1Disp; Exp(1).Dexp(1).stdP(d,dMPs(mm))=std1Disp; Exp(2).Dexp(1).stdP(d,dMPs(mm))=std1Disp; Exp(3).Dexp(1).stdP(d,dMPs(mm))=std1Disp; Exp(1).Dexp(1).minP(d,dMPs(mm))=minDisp; Exp(2).Dexp(1).minP(d,dMPs(mm))=minDisp; Exp(3).Dexp(1).minP(d,dMPs(mm))=minDisp; Exp(1).Dexp(1).maxP(d,dMPs(mm))=maxDisp; Exp(2).Dexp(1).maxP(d,dMPs(mm))=maxDisp; Exp(3).Dexp(1).maxP(d,dMPs(mm))=maxDisp; else % Need to set L r K values to zeros or pick the first spot with zeros % Spot 1 could be other than zero as the median isn't calculated if fewer than some percent are nonzero medianCalcFailedD='To Few nonZero spots for valid median RF1 selection' firstZeroDindx=find(((Exp(expN).Dexp(1).scan(rf1scNdisp).plate(1).CFout(NZrefIndxP,5))==0),1,'first'); firstZeroD=drfPindx((firstZeroDindx),dMPs(mm)); Exp(expN).Dexp(1).RFmdPindx(d,dMPs(mm))=firstZeroD; Exp(expN).Dexp(1).RFmdPpltN(d,dMPs(mm))=dMPs(mm); %RF1mp; %Is this supposed to be the MP number or the ScanPlate number??? end catch medianCalcTryFailed1D='Failed! Failed! Ln565 {Dispersed RF1s} "try" But process continued with bogus Plate spot value!' try firstZeroindxD=find(vv((chgIndx(d):chgIndx(d+1)-1),5)==0,1,'first') firstZeroPindx=uu(firstZeroindxD,4); Exp(expN).Dexp(1).RFmdPpltN(d,dMPs(mm))=dMPs(mm); Exp(expN).Dexp(1).RFmdPindx(d,dMPs(mm))=firstZeroPindx; catch catchissue='try at Ln565' Exp(expN).Dexp(1).RFmdPpltN(d,dMPs(mm))=dMPs(mm); Exp(expN).Dexp(1).RFmdPindx(d,dMPs(mm))=384; % if crapout,Then use spot384 as default to keep going end try Exp(expN).Dexp(1).RFmdPpltN=dMPs(mm); catch catchissue='Ln' Exp(expN).Dexp(1).RFmdPpltN=1; %This might keep from breaking BUT ??? end end end end % Calc. Global Mean, Std, And Median For Distributed References uu=horzcat(uu,vv); for d=1:DMnum medianIndxD=[]; NZrfIndxG=[]; minG=min(nonzeros(vv(:,d))); maxG=max(nonzeros(vv(:,d))); RFmeanG=mean(nonzeros(vv(:,d))); RFstdG=std(nonzeros(vv(:,d))); NZrfIndxG=find(vv(:,d)); % find(nonzeros(vv(:,d))); WWRONG FOR A WHILE! find(nonzeros(vv(:,d))); if isempty(RFmeanG) nonZeroCntD=0; else nonZeroCntD=length(nonzeros(vv(:,d))); end % Calc. of median requires an odd number of indx items if ~isempty(NZrfIndxG) && mod(nonZeroCntD,2)==0, medianIndxD=NZrfIndxG(1:(size(NZrfIndxG,1)-1)); end if ~isempty(NZrfIndxG) && mod(nonZeroCntD,2)~=0, medianIndxD=NZrfIndxG(:); end if ~isempty(NZrfIndxG) && length(medianIndxD) > (.15*size(drf,1)) % To calc. a median, more than 15% of spots must be nonZero mvalsDG=median(vv(medianIndxD,d)); % (nonzeros(vv(:,d))); nzPosIndxDG=find(mvalsDG==vv(:,d)); RFmdDGloc(d,1)=uu(nzPosIndxDG,1); % MP of distributedGlobal Median value RFmdDGloc(d,2)=uu(nzPosIndxDG,2); % calc r value RFmdDGloc(d,3)=uu(nzPosIndxDG,3); % calc c value lstindx=lstindx+1; lstindxOrf=lstindxOrf+1; aa(lstindx,1)={strcat('RF1mdG(',num2str(d),')')}; aa(lstindx,2)={strcat(':',num2str(RFmdDGloc(d,1)),':',num2str(RFmdDGloc(d,2)),':',num2str(RFmdDGloc(d,3)))}; %,'std_',num2str(std2))}; bb(lstindxOrf,1)={strcat('RF1mdG(',num2str(d),')')}; bb(lstindxOrf,2)={strcat(':',num2str(RFmdDGloc(d,1)),':',num2str(RFmdDGloc(d,2)),':',num2str(RFmdDGloc(d,3)))}; %,'std_',num2str(std2))}; % Composite Reference Amalgum Plot lstindx=lstindx+1; lstindxOrf=lstindxOrf+1; aa(lstindx,1)={strcat('RFcmpG(',num2str(d),')')}; aa(lstindx,2)={''}; bb(lstindxOrf,1)={strcat('RFcmpG(',num2str(d),')')}; bb(lstindxOrf,2)={''}; Exp(1).Dexp(1).RFmdGLB(d)=strcat(aa(lstindx,1),aa(lstindx,2)); Exp(2).Dexp(1).RFmdGLB(d)=strcat(aa(lstindx,1),aa(lstindx,2)); Exp(3).Dexp(1).RFmdGLB(d)=strcat(aa(lstindx,1),aa(lstindx,2)); Exp(1).Dexp(1).RFmdGindx(d)=nzPosIndxDG; Exp(2).Dexp(1).RFmdGindx(d)=nzPosIndxDG; Exp(3).Dexp(1).RFmdGindx(d)=nzPosIndxDG; Exp(1).Dexp(1).RFmdGpltN(d)=RFmdDGloc(d,1); Exp(2).Dexp(1).RFmdGpltN(d)=RFmdDGloc(d,1); Exp(3).Dexp(1).RFmdGpltN(d)=RFmdDGloc(d,1); Exp(1).Dexp(1).RFmdGscanN(d)=uu(nzPosIndxDG,4); Exp(2).Dexp(1).RFmdGscanN(d)=uu(nzPosIndxDG,4); Exp(3).Dexp(1).RFmdGscanN(d)=uu(nzPosIndxDG,4); Exp(1).Dexp(1).RFmedianG(d)=mvalsDG; Exp(2).Dexp(1).RFmedianG(d)=mvalsDG; Exp(3).Dexp(1).RFmedianG(d)=mvalsDG; Exp(1).Dexp(1).RFmeanG(d)=RFmeanG; Exp(2).Dexp(1).RFmeanG(d)=RFmeanG; Exp(3).Dexp(1).RFmeanG(d)=RFmeanG; Exp(1).Dexp(1).RFstdG(d)=RFstdG; Exp(2).Dexp(1).RFstdG(d)=RFstdG; Exp(3).Dexp(1).RFstdG(d)=RFstdG; Exp(1).Dexp(1).RFminG(d)=minG; Exp(2).Dexp(1).RFminG(d)=minG; Exp(3).Dexp(1).RFminG(d)=minG; Exp(1).Dexp(1).RFmaxG(d)=maxG; Exp(2).Dexp(1).RFmaxG(d)=maxG; Exp(3).Dexp(1).RFmaxG(d)=maxG; for ic=1:3 Exp(ic).Dexp(1).RFcmpGL.dm(d).Lvals=vv(medianIndxD,d); %17_1201 TrendOL Exp(ic).Dexp(1).RFcmpGL.dm(d).med=median(vv(medianIndxD,d)); %For Ref Composite 17_1009 Exp(ic).Dexp(1).RFcmpGL.dm(d).mean=mean(vv(medianIndxD,d)); %For Ref Composite 17_1009 Exp(ic).Dexp(1).RFcmpGL.dm(d).std=std(vv(medianIndxD,d)); %For Ref Composite 17_1009 Exp(ic).Dexp(1).RFcmpGL.dm(d).min=min(vv(medianIndxD,d)); %For Ref Composite 17_1009 Exp(ic).Dexp(1).RFcmpGL.dm(d).max=max(vv(medianIndxD,d)); %For Ref Composite 17_1009 Exp(ic).Dexp(1).RFcmpGK.dm(d).Kvals=vvK(medianIndxD,d); %17_1201 TrendOL Exp(ic).Dexp(1).RFcmpGK.dm(d).med=median(vvK(medianIndxD,d)); %For Ref Composite 17_1009 Exp(ic).Dexp(1).RFcmpGK.dm(d).mean=mean(vvK(medianIndxD,d)); %For Ref Composite 17_1009 Exp(ic).Dexp(1).RFcmpGK.dm(d).std=std(vvK(medianIndxD,d)); %For Ref Composite 17_1009 Exp(ic).Dexp(1).RFcmpGK.dm(d).min=min(vvK(medianIndxD,d)); %For Ref Composite 17_1009 Exp(ic).Dexp(1).RFcmpGK.dm(d).max=max(vvK(medianIndxD,d)); %For Ref Composite 17_1009 Exp(ic).Dexp(1).RFcmpGr.dm(d).rvals=vvr(medianIndxD,d); %17_1201 TrendOL Exp(ic).Dexp(1).RFcmpGr.dm(d).med=median(vvr(medianIndxD,d)); %For Ref Composite 17_1009 Exp(ic).Dexp(1).RFcmpGr.dm(d).mean=mean(vvr(medianIndxD,d)); %For Ref Composite 17_1009 Exp(ic).Dexp(1).RFcmpGr.dm(d).std=std(vvr(medianIndxD,d)); %For Ref Composite 17_1009 Exp(ic).Dexp(1).RFcmpGr.dm(d).min=min(vvr(medianIndxD,d)); %For Ref Composite 17_1009 Exp(ic).Dexp(1).RFcmpGr.dm(d).max=max(vvr(medianIndxD,d)); %For Ref Composite 17_1009 end end end end % End Of Disperse Reference Plataes Calculations And List Additions % Sort And Build Genelist Exp(1).Dexp(1).srtGnLst={('CheckMP/MPDMfile')}; gnLstRaw=strcat(aa(:,1),aa(:,2)); Exp(1).Dexp(1).srtGnLst=sort(gnLstRaw); Exp(2).Dexp(1).srtGnLst=Exp(1).Dexp(1).srtGnLst; Exp(3).Dexp(1).srtGnLst=Exp(1).Dexp(1).srtGnLst; orfLstRaw=strcat(bb(:,1),bb(:,2)); Exp(1).Dexp(1).srtOrfLst=sort(orfLstRaw); Exp(2).Dexp(1).srtOrfLst=Exp(1).Dexp(1).srtOrfLst; Exp(3).Dexp(1).srtOrfLst=Exp(1).Dexp(1).srtOrfLst; set(handles.listboxGnOrf,'string',Exp(1).Dexp(1).srtGnLst) catch ME %Outer loop try around entire code subroutine cd(w) set(handles.listboxGnOrf,'string',cellstr([{'Failed To Load'}; {'Check MPDMmat file'};{'Check .xls files'};{'Check \Fotos .mat files'};{'Check \matResults file'}])) %Exp(expN).Dexp(1).srtGnLst={('Failed To Load')}; Exp(1).Dexp(1).srtGnLst=cellstr([{'Failed To Load'}; {' '}]); Exp(2).Dexp(1).srtGnLst=cellstr([{'Failed To Load'}; {' '}]); Exp(3).Dexp(1).srtGnLst=cellstr([{'Failed To Load'}; {' '}]); %Exp(expN).srtOrfLst={('Failed To Load')}; Exp(1).Dexp(1).srtOrfLst=cellstr([{'Failed To Load'}; {' '}]); Exp(2).Dexp(1).srtOrfLst=cellstr([{'Failed To Load'}; {' '}]); Exp(3).Dexp(1).srtOrfLst=cellstr([{'Failed To Load'}; {' '}]); if ~contains(matFile,'.mat') ExpOutImFile=fullfile(matFile); EZvImagesOnly end end expN=1; % Fix 170424 DexpN=1; EZdiagRFsSheet Exp(1).Dexp(1).RFrnames=rnames; Exp(2).Dexp(1).RFrnames=rnames; Exp(3).Dexp(1).RFrnames=rnames; Exp(1).Dexp(1).RFdata=data; Exp(2).Dexp(1).RFdata=data; Exp(3).Dexp(1).RFdata=data; RFconfig=0; if sum(ismember(fieldnames(Exp(expN).Dexp(1)),'RFmean')) ... && ~isempty(Exp(expN).Dexp(1).RFmean) ... && sum(ismember(fieldnames(Exp(expN).Dexp(1)),'RFmeanG')) ... && isempty(Exp(expN).Dexp(1).RFmeanG) RFconfig=1; end if sum(ismember(fieldnames(Exp(expN).Dexp(1)),'RFmean'))==0 ... || isempty(Exp(expN).Dexp(1).RFmean) ... && sum(ismember(fieldnames(Exp(expN).Dexp(1)),'RFmeanG')) ... && ~isempty(Exp(expN).Dexp(1).RFmeanG) RFconfig=2; end if sum(ismember(fieldnames(Exp(expN).Dexp(1)),'RFmean')) ... && ~isempty(Exp(expN).Dexp(1).RFmean) ... && sum(ismember(fieldnames(Exp(expN).Dexp(1)),'RFmeanG')) ... && ~isempty(Exp(expN).Dexp(1).RFmeanG) RFconfig=3; end Exp(1).RFconfig=RFconfig; Exp(2).RFconfig=RFconfig; Exp(3).RFconfig=RFconfig; % ZoneRelated try msgBadGeneName catch end try Exp(1).Dexp(1).resetHtmpTg=3; Exp(2).Dexp(1).resetHtmpTg=3; Exp(3).Dexp(1).resetHtmpTg=3; % ZoneRelated if RFconfig==0 RFconfigMsg='No RF1 references in this Experiment Job' Exp(1).resetHtmpTg=3; Exp(2).resetHtmpTg=3; Exp(3).resetHtmpTg=3; % ZoneRelated end catch end prntHt=0; % HtMapTog 'string', 'Current user HL/HN' selection' try Exp(1).htmapPBsel=0; set(handles.HtMapTog1,'string','L') Exp(2).htmapPBsel=0; %ZoneRelated set(handles.HtMapTog2,'string','L') Exp(3).htmapPBsel=0; set(handles.HtMapTog3,'string','L') catch % expN=1; end for ic=1:3 Expaa{ic,1}=aa; Expbb{ic,1}=bb; end htMapTogPBfg=0; Exp(1).htmapRFanswer={'G','L'}; % ZoneRelated Exp(2).htmapRFanswer={'G','L'}; Exp(3).htmapRFanswer={'G','L'}; zonePB=expN; % added 230828 to fix migration ghandles=handles; ghandles.guiFig=gcf; Exp(4).guiFig=gcf;