%EZlstBoxExt.m % Calls either EZdestComp.m % or if 'Chrono' EZmDayPlotUCmp.m and EZmDayTrend.m and if ghandles.CompositeTog_,EZmDayPlotUcomposite % or by EZviewGui.m (listboxGnOrf_Callback) % Replace get(ghandles.CompositeTog1,'value')==1 ... with get(handles.... if expN==1 && get(handles.CompositeTog1,'value')==1 ... || expN==2 && get(handles.CompositeTog2,'value')==1 ... || expN==3 && get(handles.CompositeTog3,'value')==1 CompositeTrendFlg=1; else CompositeTrendFlg=0; end Exp(expN).cLmdEven=0; % Added for Cummulative Median and Mean 'C' plot RFDMflg=0; if zoneSel==1,DexpN=(get(handles.DN1,'value')); end if zoneSel==2,DexpN=(get(handles.DN2,'value')); end if zoneSel==3,DexpN=(get(handles.DN3,'value')); end try p1=cell2mat(strfind(selGnOrf,'(')); p2=cell2mat(strfind(selGnOrf,')')); dmNum=str2num(selGnOrf{1}(p1+1:p2-1)); inDM=selGnOrf{1}(p1+1:p2-1); if (~isempty(dmNum)&& isnumeric(dmNum)),RFDMflg=1; end catch end if RFDMflg, Exp(zoneSel).Dexp(DexpN).pertSel=dmNum; end % pertSel=pert; inMP=selGnOrf{1}((LBdlims{:,:}(1))+1:(LBdlims{:,:}(2))-1) % LBmp=str2double(inMP); sgdInfoOnly=get(handles.InfoToggle,'value'); if sgdInfoOnly==1 EZviewInfoBox else % Runs to end of EZlstBoxExt.m within this else condition if zoneSel==1 Max=floor(get(handles.MPsldr1,'max')); Min=floor(get(handles.MPsldr1,'min')); if str2num(inMP) >=Min && str2num(inMP) <=Max set(handles.MPed1,'string',inMP) set(handles.MPsldr1,'value',str2double(inMP)); else if str2num(inMP) >=Max, set(handles.MPsldr1,'value',Max); set(handles.MPed1,'string',num2str(Max));end if str2num(inMP) <=Min, set(handles.MPsldr1,'value',Min),set(handles.MPed1,'string',num2str(Min));end end end if zoneSel==2 Max=floor(get(handles.MPsldr2,'max')); Min=floor(get(handles.MPsldr2,'min')); if str2num(inMP) >=Min && str2num(inMP) <=Max set(handles.MPed2,'string',inMP) set(handles.MPsldr2,'value',str2double(inMP)); else if str2num(inMP) >=Max, set(handles.MPsldr2,'value',Max); set(handles.MPed2,'string',num2str(Max));end if str2num(inMP) <=Min, set(handles.MPsldr2,'value',Min),set(handles.MPed2,'string',num2str(Min));end end end if zoneSel==3 Max=floor(get(handles.MPsldr3,'max')); Min=floor(get(handles.MPsldr3,'min')); if str2num(inMP) >=Min && str2num(inMP) <=Max set(handles.MPed3,'string',inMP) set(handles.MPsldr3,'value',str2double(inMP)); else if str2num(inMP) >=Max, set(handles.MPsldr3,'value',Max); set(handles.MPed3,'string',num2str(Max));end if str2num(inMP) <=Min, set(handles.MPsldr3,'value',Min),set(handles.MPed3,'string',num2str(Min));end end end if RFDMflg if zoneSel==1 Max=floor(get(handles.DMsldr1,'max')); Min=floor(get(handles.DMsldr1,'min')); if str2num(inDM) >=Min && str2num(inDM) <=Max set(handles.DMed1,'string',inDM) set(handles.DMsldr1,'value',str2double(inDM)); else if str2num(inDM) >=Max, set(handles.DMsldr1,'value',Max); set(handles.DMed1,'string',num2str(Max));end if str2num(inDM) <=Min, set(handles.DMsldr1,'value',Min),set(handles.DMed1,'string',num2str(Min));end end end if zoneSel==2 Max=floor(get(handles.DMsldr2,'max')); Min=floor(get(handles.DMsldr2,'min')); if str2num(inDM) >=Min && str2num(inDM) <=Max set(handles.DMed2,'string',inDM) set(handles.DMsldr2,'value',str2double(inDM)); else if str2num(inDM) >=Max, set(handles.DMsldr2,'value',Max); set(handles.DMed2,'string',num2str(Max));end if str2num(inDM) <=Min, set(handles.DMsldr2,'value',Min),set(handles.DMed2,'string',num2str(Min));end end end if zoneSel==3 Max=floor(get(handles.DMsldr3,'max')); Min=floor(get(handles.DMsldr3,'min')); if str2num(inDM) >=Min && str2num(inDM) <=Max set(handles.DMed3,'string',inDM) set(handles.DMsldr3,'value',str2double(inDM)); else if str2num(inDM) >=Max, set(handles.DMsldr3,'value',Max); set(handles.DMed3,'string',num2str(Max));end if str2num(inDM) <=Min, set(handles.DMsldr3,'value',Min),set(handles.DMed3,'string',num2str(Min));end end end end EZVimDisplay % 17_111 If CompositeTog_ set, Routine called to find all gene replicates and produce composite values if CompositeTrendFlg==1 if strcmp(Exp(expN).DexpType, 'chrono') EZmDayPlotUcmp else EZplotUcmp end % Plot Selected Gene/Orf Spot % if non-Composite ("normal") then - ... elseif CompositeTrendFlg~=1 destPerMP=Exp(zoneSel).Dexp(DexpN).destPerMP; %length(gS.DM1.drug); pertSel=Exp(zoneSel).Dexp(DexpN).pertSel; %floor(get(handles.DMsldr1,'value')); plateNum=(LBmp-1)*destPerMP + pertSel; indx=((LBr-1)*24) +LBc MP=Exp(zoneSel).Dexp(DexpN).MP; try K=scan(1,plateNum).plate(1).CFout(indx,3); Ks=num2str(K); r=scan(1,plateNum).plate(1).CFout(indx,4); rs=num2str(r); l=scan(1,plateNum).plate(1).CFout(indx,5); Ls=num2str(l); try Kstr=Ks(1:5); catch, Kstr=Ks(1:length(Ks)); end try rstr=rs(1:5); catch, rstr=rs(1:length(rs)); end try Lstr=Ls(1:5); catch, Lstr=Ls(1:length(Ls)); end Kl=scan(1,plateNum).plate(1).CFout(indx,7); Ku=scan(1,plateNum).plate(1).CFout(indx,8); rl=scan(1,plateNum).plate(1).CFout(indx,9); ru=scan(1,plateNum).plate(1).CFout(indx,10); lfast=scan(1,plateNum).plate(1).CFout(indx,11); lslow=scan(1,plateNum).plate(1).CFout(indx,12); t=1:200; clear g; try g=K ./ (1 + exp(-r.* (t - l ))); gSlow=Kl ./ (1 + exp(-rl.* (t - lslow ))); gFast=Ku ./ (1 + exp(-ru.* (t - lfast ))); if K==0||r==0||l==0, g(1:200)=1;gSlow(1:200)=1;gFast(1:200)=1;end tser=(scan(1,plateNum).plate(1).t0Series(:)); rawData=scan(1,plateNum).plate(1).intens(indx,:)/scan(1,plateNum).plate(1).Ag(indx); if zoneSel==1, plotAxes=ghandles.Paxes1; OLaxes=ghandles.OLaxes1; Dlaxes=ghandles.Dlaxes1;Dkaxes=ghandles.Dkaxes1;Draxes=ghandles.Draxes1; Exp(1).traceN=Exp(1).traceN+1; traceN=Exp(1).traceN; end if zoneSel==2, plotAxes=ghandles.Paxes2; OLaxes=ghandles.OLaxes2;Daxes=ghandles.Daxes2; Dlaxes=ghandles.Dlaxes2;Dkaxes=ghandles.Dkaxes2;Draxes=ghandles.Draxes2; Exp(2).traceN=Exp(2).traceN+1; traceN=Exp(2).traceN; end if zoneSel==3, plotAxes=ghandles.Paxes3; OLaxes=ghandles.OLaxes3;Daxes=ghandles.Daxes3; Dlaxes=ghandles.Dlaxes3;Dkaxes=ghandles.Dkaxes3;Draxes=ghandles.Draxes3; Exp(3).traceN=Exp(3).traceN+1; traceN=Exp(3).traceN; end plot(plotAxes,t,g);hold (plotAxes,'on');plot(plotAxes,tser,rawData,'g*'); plot(plotAxes,t,gSlow,'y');plot(plotAxes,t,gFast,'r');hold (plotAxes,'off'); % Store L R and K valves for Composite [C] plots Exp(expN).ll(traceN)=l; Exp(expN).rr(traceN)=r; Exp(expN).kk(traceN)=K; Exp(expN).lslow(traceN)=lslow; Exp(expN).lfast(traceN)=lfast; try for i=1:length(Exp(zoneSel).hOL(:)),set(Exp(zoneSel).hOL(i),'color',[0 0 1]); end, catch; end Exp(zoneSel).hOL(traceN)=plot(OLaxes,t,g);hold on; set(Exp(zoneSel).hOL(traceN),'color',[1 0 0]) Exp(zoneSel).hOLb(traceN)=plot(OLaxes,tser,rawData,'g*'); catch catchissue='Ln100 EZlstBoxExt' end % Get the DM agar description if expN==1,DMstr=char(get(handles.DM1,'string'));end if expN==2,DMstr=char(get(handles.DM2,'string'));end if expN==3,DMstr=char(get(handles.DM3,'string'));end if isequal(selGnOrf{1}(1:3),'RF1') gene={selGnOrf{1}(1:p2)}; geneOrfstr=strcat(gene,'_','m',num2str(LBmp),'r',num2str(LBr),'c',num2str(LBc),'_',tPtStr); grfgenestr=strcat(gene,'_','m',num2str(LBmp),'r',num2str(LBr),'c',num2str(LBc)); else gene=MP(1,LBmp).genename{1,1}(indx); orf=MP(1,LBmp).orf{1,1}(indx); geneOrfstr=strcat(gene,'_',orf,'_','m',num2str(LBmp),'r',num2str(LBr),'c',num2str(LBc),'_',tPtStr); grfgenestr=strcat(gene,'_',orf,'_','m',num2str(LBmp),'r',num2str(LBr),'c',num2str(LBc)); end spec=MP(1,LBmp).specifics{1,1}(indx); %orfrep=MP(1,plateNum).orfRep{1,1}(indx); tPtStr=strcat('T=', num2str(scan(1,plateNum).plate(1).t0Series(tPtSel))); graphStr=strcat(grfgenestr,'_','L=',Lstr,'_','r=',rstr,'_','K=',Kstr); spotDescrip=strcat(graphStr,'->',DMstr); xp=char(Exp(zoneSel).Dexp(DexpN).resDir); if ispc, slashPos=strfind(char(Exp(zoneSel).Dexp(DexpN).resDir),'\'); else slashPos=strfind(char(Exp(zoneSel).Dexp(DexpN).resDir),'/'); end startPos=slashPos(length(slashPos)-1) +1; endPos=slashPos(length(slashPos)) -1 expStr={xp(startPos:endPos)} Exp(zoneSel).hOLname(traceN)=spotDescrip; % graphStr; %ghandles.Exp(zoneSel).hOLname(traceN)=graphStr; Exp(zoneSel).hOLexpNm(traceN)=expStr; % ghandles.Exp(expN).hOLexpNm(traceN)=expStr; Exp(zoneSel).hOLresDir(traceN)={Exp(zoneSel).Dexp(DexpN).resDir}; Exp(zoneSel).hOLplateNum(traceN)=plateNum; % traceData=vertcat(get(Exp(OLay).hOL(traceN),'XData'),get(ghandles.Exp(OLay).hOL(traceN),'YData')); if zoneSel==1,set(ghandles.GeneOrfLoc1,'string',geneOrfstr);end % Displays the value. if zoneSel==2,set(ghandles.GeneOrfLoc2,'string',geneOrfstr);end if zoneSel==3,set(ghandles.GeneOrfLoc3,'string',geneOrfstr);end if zoneSel==1,set(ghandles.graphStrLoc1,'string',spotDescrip);end %graphStr);end % Displays the value. if zoneSel==2,set(ghandles.graphStrLoc2,'string',spotDescrip);end %graphStr);end if zoneSel==3,set(ghandles.graphStrLoc3,'string',spotDescrip);end %graphStr);end catch catchissue='Ln141 EZlstBoxExt' msg='Error' end end % Write Spot and Exp Info to OLay title areas if zoneSel==1 %&& get(ghandles.rotPB1,'value')~=1 if length(char(Exp(zoneSel).hOLname(traceN)))>40,set(ghandles.OLay1,'FontSize',8);end set(ghandles.OLay1,'string', Exp(zoneSel).hOLname(traceN)); if length(char(Exp(zoneSel).hOLexpNm(traceN)))>40,set(ghandles.OLexp1,'FontSize',8);end set(ghandles.OLexp1,'string',Exp(zoneSel).hOLexpNm(traceN)); end if zoneSel==2 %&& get(ghandles.rotPB2,'value') ~=1 if length(char(Exp(zoneSel).hOLname(traceN)))>40,set(ghandles.OLay2,'FontSize',8);end set(ghandles.OLay2,'string', Exp(zoneSel).hOLname(traceN)); if length(char(Exp(zoneSel).hOLexpNm(traceN)))>40,set(ghandles.OLexp2,'FontSize',8);end set(ghandles.OLexp2,'string',Exp(zoneSel).hOLexpNm(traceN)); end if zoneSel==3 %&& get(ghandles.rotPB3,'value') ~=1 if length(char(Exp(zoneSel).hOLname(traceN)))>40,set(ghandles.OLay3,'FontSize',8);end set(ghandles.OLay3,'string', Exp(OLay).hOLname(traceN)); if length(char(Exp(zoneSel).hOLexpNm(traceN)))>40,set(ghandles.OLexp3,'FontSize',8);end set(ghandles.OLexp3,'string',Exp(OLay).hOLexpNm(traceN)); end % Highlight gene/orf select Spot on Image tPtSel=Exp(zoneSel).Dexp(DexpN).tPtSel; Rim=16-(LBr-1); Cim =LBc; spotCoor=Exp(zoneSel).Dexp(DexpN).FexpScanSpots{1,plateNum}{Cim,Rim,tPtSel}; if zoneSel==1, expAxes=ghandles.Iaxes1;end if zoneSel==2, expAxes=ghandles.Iaxes2;end if zoneSel==3, expAxes=ghandles.Iaxes3;end %axes(ghandles.Iaxes1) plot(expAxes,(spotCoor(2)+24),(spotCoor(1)+24),'.y') % GraphicDestinationPerturbationComparison**15_0821 EZdatatip=0; %** if strcmp((Exp(expN).DexpType),'single') ... || strcmp((Exp(expN).DexpType),'multi') EZdestComp elseif strcmp((Exp(expN).DexpType),'chrono') EZmDayTrend end % HeatMap Text Field try ll=num2str(l); if length(ll)>5,htl=ll(1:5);else htl=ll;end catch htl=' '; end try n1=num2str(Exp(expN).Dexp(DexpN).HtMpIntN1(indx)); if length(n1)>5,N1=n1(1:5);else N1=n1; end htN1=strcat('_N1=',N1); catch, htN1=' '; end try htmapStr=strcat('L=',htl, htN1) if expN==1,set(ghandles.HtMapVals1,'string',htmapStr);end if expN==2,set(ghandles.HtMapVals2,'string',htmapStr);end if expN==3,set(ghandles.HtMapVals3,'string',htmapStr);end catch end end