S000000002	YAL002W	VPS8	Vacuolar Protein Sorting	143707	147531	1	Membrane-binding component of the CORVET complex	Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif
S000000003	YAL003W	EFB1	Elongation Factor Beta	142174	143160	1	Translation elongation factor 1 beta	Translation elongation factor 1 beta; stimulates nucleotide exchange to regenerate EF-1 alpha-GTP for the next elongation cycle; part of the EF-1 complex, which facilitates binding of aminoacyl-tRNA to the ribosomal A site; human homolog EEF1B2 can complement yeast efb1 mutants
S000000004	YAL005C	SSA1	Stress-Seventy subfamily A	139503	141431	-1	ATPase involved in protein folding and NLS-directed nuclear transport	ATPase involved in protein folding and NLS-directed nuclear transport; member of HSP70 family; required for ubiquitin-dependent degradation of short-lived proteins; forms chaperone complex with Ydj1p; localized to nucleus, cytoplasm, cell wall; 98% identical to paralog Ssa2p with different functional specificity in propagation of yeast [URE3] prions, vacuolar-mediated degradations of gluconeogenesis enzymes; general targeting factor of Hsp104p to prion fibrils
S000000005	YAL007C	ERP2	Emp24p/Erv25p Related Protein	137698	138345	-1	Member of the p24 family involved in ER to Golgi transport	Member of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; role in misfolded protein quality control; forms a heterotrimeric complex with Erp1p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP2 has a paralog, ERP4, that arose from the whole genome duplication
S000000006	YAL008W	FUN14	Function Unknown Now	136914	137510	1	Integral mitochondrial outer membrane (MOM) protein	Integral mitochondrial outer membrane (MOM) protein; dosage suppressor of an MDM10 null that reduces ERMES-related phenotypes, such as alterations in mitochondrial morphology, protein complex assembly, and lipid profile; dosage suppressor of MDM12, MDM34, and MMM1 null mutant growth defects; novel mechanism of MOM import involving Tom70p, the TOM complex, and the TIM23 complex, requiring mitochondrial membrane potential and processing by the IMP complex for correct biogenesis
S000000007	YAL009W	SPO7	SPOrulation	135854	136633	1	Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme	Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear/ER membrane association and activation of Pah1p, a phosphatidate phosphatase involved in the production of diacylglycerol DAG for lipid droplet biogenesis; phosphatase activity of the Nem1p-Spo7p complex is inhibited by Ice2p; regulates nuclear growth by controlling phospholipid biosynthesis; required for normal nuclear envelope morphology, premeiotic replication, and sporulation
S000000008	YAL010C	MDM10	Mitochondrial Distribution and Morphology	134184	135665	-1	Subunit of both the ERMES and the SAM complex	Subunit of both the ERMES and the SAM complex; component of ERMES complex which acts as a molecular tether between the mitochondria and the ER, necessary for efficient phospholipid exchange between organelles and for mitophagy; SAM/TOB complex component that functions in the assembly of outer membrane beta-barrel proteins; involved in mitochondrial inheritance and morphology; ERMES complex is often co-localized with peroxisomes and concentrated areas of pyruvate dehydrogenase
S000000009	YAL011W	SWC3	SWr Complex	132199	134076	1	Protein of unknown function	Protein of unknown function; component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for formation of nuclear-associated array of smooth endoplasmic reticulum known as karmellae
S000000010	YAL012W	CYS3	CYStathionine gamma-lyase	130799	131983	1	Cystathionine gamma-lyase	Cystathionine gamma-lyase; catalyzes one of the two reactions involved in the transsulfuration pathway that yields cysteine from homocysteine with the intermediary formation of cystathionine; protein abundance increases in response to DNA replication stress
S000000011	YAL013W	DEP1	Disability in regulation of Expression of genes involved in Phospholipid biosynthesis	129270	130487	1	Component of the Rpd3L histone deacetylase complex	Component of the Rpd3L histone deacetylase complex; required for diauxic shift-induced histone H2B deposition onto rDNA genes; transcriptional modulator involved in regulation of structural phospholipid biosynthesis genes and metabolically unrelated genes, as well as maintenance of telomeres, mating efficiency, and sporulation; localizes to both the nucleus and mitochondria where it may play a role in mitophagy
S000000012	YAL014C	SYN8	SYNtaxin	128252	129019	-1	Endosomal SNARE related to mammalian syntaxin 8	Endosomal SNARE related to mammalian syntaxin 8
S000000013	YAL015C	NTG1	eNdonuclease Three-like Glycosylase	126903	128102	-1	DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase	DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; required for maintaining mitochondrial genome integrity; NTG1 has a paralog, NTG2, that arose from the whole genome duplication
S000000014	YAL016W	TPD3	tRNA Processing Deficient	124879	126786	1	Regulatory subunit A of the heterotrimeric PP2A complex	Regulatory subunit A of the heterotrimeric PP2A complex; the heterotrimeric protein phosphatase 2A (PP2A) complex also contains regulatory subunit Cdc55p and either catalytic subunit Pph21p or Pph22p; required for cell morphogenesis and transcription by RNA polymerase III
S000000015	YAL017W	PSK1	Pas domain-containing Serine/threonine protein Kinase	120225	124295	1	PAS domain-containing serine/threonine protein kinase	PAS domain-containing serine/threonine protein kinase; coordinately regulates protein synthesis and carbohydrate metabolism and storage in response to a unknown metabolite that reflects nutritional status; PSK1 has a paralog, PSK2, that arose from the whole genome duplication
S000000016	YAL018C	LDS1	Lipid Droplets in Sporulation	118564	119541	-1	Protein Involved in spore wall assembly	Protein Involved in spore wall assembly; localizes to lipid droplets found on or outside of the prospore membrane; shares similarity with Lds2p and Rrt8p, and a strain mutant for all 3 genes exhibits reduced dityrosine fluorescence relative to the single mutants
S000000017	YAL019W	FUN30	Function Unknown Now	114919	118314	1	Snf2p family member with ATP-dependent chromatin remodeling activity	Snf2p family member with ATP-dependent chromatin remodeling activity; has a role in silencing at the mating type locus, telomeres and centromeres; enriched at centromeres and is required for correct chromatin structure around centromeres, as well as at the boundary element of the silent HMR; recruited to DNA double-strand breaks (DSBs) where it promotes 5' strand resection of DSBs; potential Cdc28p substrate
S000000018	YAL020C	ATS1	Alpha Tubulin Suppressor	113614	114615	-1	Protein required for modification of wobble nucleosides in tRNA	Protein required for modification of wobble nucleosides in tRNA; acts with Elongator complex, Kti11p, and Kti12p; has a potential role in regulatory interactions between microtubules and the cell cycle; forms a stable heterodimer with Kti11p
S000000019	YAL021C	CCR4	Carbon Catabolite Repression	110846	113359	-1	Component of the CCR4-NOT transcriptional complex	Component of the CCR4-NOT transcriptional complex; CCR4-NOT is involved in regulation of gene expression; component of the major cytoplasmic deadenylase, which is involved in mRNA poly(A) tail shortening
S000000020	YAL022C	FUN26	Function Unknown Now	108877	110430	-1	High affinity, broad selectivity, nucleoside/nucleobase transporter	High affinity, broad selectivity, nucleoside/nucleobase transporter; vacuolar membrane localized transporter which may regulate the balance of nicotinamide riboside (NmR) levels between the cytosol and vacuole, contributing to salvage of NmR for use in cytosolic NAD+ synthesis; equilibrative nucleoside transporter (ENT) family member
S000000021	YAL023C	PMT2	Protein O-MannosylTransferase	106272	108551	-1	Protein O-mannosyltransferase of the ER membrane	Protein O-mannosyltransferase of the ER membrane; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; involved in ER quality control; functions as a heterodimer with Pmt2p but can also pair with Pmt5p; antifungal drug target; PMT2 has a paralog, PMT3, that arose from the whole genome duplication
S000000022	YAL024C	LTE1	Low Temperature Essential	101565	105872	-1	Protein similar to GDP/GTP exchange factors	Protein similar to GDP/GTP exchange factors; without detectable GEF activity; required for asymmetric localization of Bfa1p at daughter-directed spindle pole bodies and for mitotic exit at low temperatures
S000000023	YAL025C	MAK16	MAintenance of Killer	100225	101145	-1	Essential nuclear protein	Essential nuclear protein; constituent of 66S pre-ribosomal particles; required for maturation of 25S and 5.8S rRNAs; required for maintenance of M1 satellite double-stranded RNA of the L-A virus
S000000024	YAL026C	DRS2	Deficiency of Ribosomal Subunits	95630	99697	-1	Trans-golgi network aminophospholipid translocase (flippase)	Trans-golgi network aminophospholipid translocase (flippase); P4-type ATPase; maintains membrane lipid asymmetry in post-Golgi secretory vesicles; contributes to clathrin-coated vesicle formation, endocytosis, protein trafficking between the Golgi and endosomal system and the cellular response to mating pheromone; autoinhibited by its C-terminal tail; localizes to the trans-Golgi network
S000000025	YAL027W	SAW1	Single-strand Annealing Weakened	94687	95472	1	5'- and 3'-flap DNA binding protein	5'- and 3'-flap DNA binding protein; required for recruitment of Rad1p-Rad10p to single-strand annealing intermediates with 3' non-homologous tails for removal during double-strand break repair; forms a complex with Rad1p-Rad10p and stimulates its endonuclease activity; green fluorescent protein (GFP)-fusion protein localizes to the nucleus
S000000026	YAL028W	FRT2	Functionally Related to TCP1	92900	94486	1	Tail-anchored ER membrane protein of unknown function	Tail-anchored ER membrane protein of unknown function; interacts with homolog Frt1p; promotes growth in conditions of high Na+, alkaline pH, or cell wall stress, possibly via a role in posttranslational translocation; potential Cdc28p substrate; FRT2 has a paralog, FRT1, that arose from the whole genome duplication
S000000027	YAL029C	MYO4	MYOsin	87855	92270	-1	Type V myosin motor involved in actin-based transport of cargos	Type V myosin motor involved in actin-based transport of cargos; required for mRNA transport, including ASH1 mRNA, and facilitating the growth and movement of ER tubules into the growing bud along with She3p; MYO4 has a paralog, MYO2, that arose from the whole genome duplication
S000000028	YAL030W	SNC1	Suppressor of the Null allele of CAP	87286	87752	1	Vesicle membrane receptor protein (v-SNARE)	Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; proposed to be involved in endocytosis; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC1 has a paralog, SNC2, that arose from the whole genome duplication
S000000029	YAL031C	GIP4	Glc7 Interacting Protein	84749	87031	-1	Cytoplasmic protein that regulates protein phosphatase 1 Glc7p	Cytoplasmic protein that regulates protein phosphatase 1 Glc7p; protein overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; potential Cdc28p substrate
S000000030	YAL032C	PRP45	Pre-mRNA Processing	83335	84474	-1	Protein required for pre-mRNA splicing	Protein required for pre-mRNA splicing; associates with the spliceosome and interacts with splicing factors Prp22p and Prp46p; orthologous to human transcriptional coactivator SKIP and can activate transcription of a reporter gene
S000000031	YAL033W	POP5	Processing Of Precursor RNAs	82706	83227	1	Subunit of both RNase MRP and nuclear RNase P	Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs
S000000032	YAL034W-A	MTW1	Mis TWelve-like	79718	80587	1	Essential component of the MIND kinetochore complex	Essential component of the MIND kinetochore complex; joins kinetochore subunits contacting DNA to those contacting microtubules; critical to kinetochore assembly; complex consists of Mtw1p Including Nnf1p-Nsl1p-Dsn1p (MIND)
S000000033	YAL035W	FUN12	Function Unknown Now	76427	79435	1	Translation initiation factor eIF5B	Translation initiation factor eIF5B; GTPase that promotes Met-tRNAiMet binding to ribosomes and ribosomal subunit joining; promotes GTP-dependent maturation of 18S rRNA by Nob1p; protein abundance increases in response to DNA replication stress; homolog of bacterial IF2
S000000034	YAL036C	RBG1	RiBosome interacting Gtpase	75043	76152	-1	Member of the DRG family of GTP-binding proteins	Member of the DRG family of GTP-binding proteins; associates with translating ribosomes; interacts with Tma46p, Ygr250cp, Gir2p and Yap1p via two-hybrid; localizes to cytosol and peroxisomes
S000000035	YAL037W	""	""	74020	74823	1	Putative protein of unknown function	Putative protein of unknown function; YAL037W has a paralog, YOR342C, that arose from the whole genome duplication
S000000036	YAL038W	CDC19	Cell Division Cycle	71786	73288	1	Pyruvate kinase	Pyruvate kinase; functions as a homotetramer in glycolysis to convert phosphoenolpyruvate to pyruvate, the input for aerobic (TCA cycle) or anaerobic (glucose fermentation) respiration; regulated via allosteric activation by fructose bisphosphate; CDC19 has a paralog, PYK2, that arose from the whole genome duplication
S000000037	YAL039C	CYC3	CYtochrome C	68716	69525	-1	Cytochrome c heme lyase (holocytochrome c synthase)	Cytochrome c heme lyase (holocytochrome c synthase); attaches heme to apo-cytochrome c (Cyc1p or Cyc7p) in mitochondrial intermembrane space; human homolog HCCS implicated in microphthalmia with linear skin defects (MLS), and can complement yeast null mutant
S000000038	YAL040C	CLN3	CycLiN	65778	67520	-1	G1 cyclin involved in cell cycle progression	G1 cyclin involved in cell cycle progression; activates Cdc28p kinase to promote G1 to S phase transition; plays a role in regulating transcription of other G1 cyclins, CLN1 and CLN2; regulated by phosphorylation and proteolysis; acetyl-CoA induces CLN3 transcription in response to nutrient repletion to promote cell-cycle entry; cell cycle arrest phenotype of the cln1 cln2 cln3 triple null mutant is complemented by any of human cyclins CCNA2, CCNB1, CCNC, CCND1, or CCNE1
S000000039	YAL041W	CDC24	Cell Division Cycle	62840	65404	1	Guanine nucleotide exchange factor (GEF) for Cdc42p	Guanine nucleotide exchange factor (GEF) for Cdc42p; required for polarity establishment and maintenance, and mutants have morphological defects in bud formation and shmooing; relocalizes from nucleus to cytoplasm upon DNA replication stress; thermosensitivity of the cdc24-4 mutant in the presence of sorbitol is functionally complemented by human CDC42
S000000040	YAL042W	ERV46	ER Vesicle	61316	62563	1	Protein localized to COPII-coated vesicles	Protein localized to COPII-coated vesicles; forms a complex with Erv41p; involved in the membrane fusion stage of transport
S000000041	YAL043C	PTA1	Pre-Trna Accumulation	58695	61052	-1	Subunit of holo-CPF	Subunit of holo-CPF; holo-CPF is a multiprotein complex and functional homolog of mammalian CPSF, required for the cleavage and polyadenylation of mRNA and snoRNA 3' ends; involved in pre-tRNA processing; binds to the phosphorylated CTD of RNAPII
S000000042	YAL044C	GCV3	GlyCine cleaVage	57950	58462	-1	H subunit of the mitochondrial glycine decarboxylase complex	H subunit of the mitochondrial glycine decarboxylase complex; glycine decarboxylase is required for the catabolism of glycine to 5,10-methylene-THF; also required for all protein lipoylation; expression is regulated by levels of 5,10-methylene-THF
S000000043	YAL045C	""	""	57488	57796	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps YAL044W-A
S000000044	YAL046C	BOL3	BolA-like protein	57029	57385	-1	Protein involved in Fe-S cluster transfer to mitochondrial clients	Protein involved in Fe-S cluster transfer to mitochondrial clients; protects [4Fe-4S] clusters from damage due to oxidative stress by acting along with Nfu1p at a late step in the transfer of [4Fe-4S] clusters from the ISA complex to mitochondrial client proteins like lipoate synthase and succinate dehydrogenase; sequence similarity to human BOLA family member, BOLA3, mutations of which are associated with Multiple Mitochondria Dysfunctions Syndrome (MMDS2)
S000000045	YAL047C	SPC72	Spindle Pole Component	54989	56857	-1	Gamma-tubulin small complex (gamma-TuSC) receptor	Gamma-tubulin small complex (gamma-TuSC) receptor; recruits the gamma-TuSC complex to the cytoplasmic side of the SPB, connecting nuclear microtubules to the SPB; involved in astral microtubule formation, stabilization, and with Stu2p, anchoring astral MTs at the cytoplasmic face of the SPB, and regulating plus-end MT dynamics; regulated by Cdc5 kinase
S000000046	YAL048C	GEM1	GTPase EF-hand protein of Mitochondria	52801	54789	-1	Outer mitochondrial membrane GTPase, subunit of the ERMES complex	Outer mitochondrial membrane GTPase, subunit of the ERMES complex; potential regulatory subunit of the ERMES complex that links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth; cells lacking Gem1p contain collapsed, globular, or grape-like mitochondria; ortholog of metazoan Miro GTPases
S000000047	YAL049C	AIM2	Altered Inheritance rate of Mitochondria	51855	52595	-1	Cytoplasmic protein involved in mitochondrial function or organization	Cytoplasmic protein involved in mitochondrial function or organization; null mutant displays reduced frequency of mitochondrial genome loss; potential Hsp82p interactor
S000000048	YAL051W	OAF1	Oleate-Activated transcription Factor	48564	51707	1	Oleate-activated transcription factor	Oleate-activated transcription factor; subunit of a heterodimeric complex with Pip2p, which binds to oleate-response elements (ORE) in the promoter of genes involved in beta-oxidation of fatty acids, peroxisome organization and biogenesis, activating transcription in the presence of oleate; regulates chromatin silencing at telomeres; involved in diauxic shift; OAF1 has a paralog, PIP2, that arose from the whole genome duplication
S000000049	YAL053W	FLC2	FLavin Carrier	45899	48250	1	Putative calcium channel involved in calcium release under hypotonic s	Putative calcium channel involved in calcium release under hypotonic stress; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; targeted to vacuole via AP-3 pathway; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication
S000000050	YAL054C	ACS1	Acetyl CoA Synthetase	42881	45022	-1	Acetyl-coA synthetase isoform	Acetyl-coA synthetase isoform; along with Acs2p, acetyl-coA synthetase isoform is the nuclear source of acetyl-coA for histone acetylation; expressed during growth on nonfermentable carbon sources and under aerobic conditions
S000000051	YAL055W	PEX22	PEroXisome related	42177	42719	1	Putative peroxisomal membrane protein	Putative peroxisomal membrane protein; required for import of peroxisomal proteins; functionally complements a Pichia pastoris pex22 mutation
S000000052	YAL056W	GPB2	""	39259	41901	1	Multistep regulator of cAMP-PKA signaling	Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; inhibits Ras activity through direct interactions with Ira1p/2p; regulated by G-alpha protein Gpa2p; GPB2 has a paralog, GPB1, that arose from the whole genome duplication
S000000054	YAL058W	CNE1	CalNExin and calreticulin homolog	37464	38972	1	Calnexin	Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast
S000000055	YAL059W	ECM1	ExtraCellular Mutant	36509	37147	1	Pre-ribosomal factor involved in 60S ribosomal protein subunit export	Pre-ribosomal factor involved in 60S ribosomal protein subunit export; associates with the pre-60S particle; shuttles between the nucleus and cytoplasm
S000000056	YAL060W	BDH1	Butanediol DeHydrogenase	35155	36303	1	NAD-dependent (R,R)-butanediol dehydrogenase	NAD-dependent (R,R)-butanediol dehydrogenase; catalyzes oxidation of (R,R)-2,3-butanediol to (3R)-acetoin, oxidation of meso-butanediol to (3S)-acetoin, and reduction of acetoin; enhances use of 2,3-butanediol as an aerobic carbon source
S000000057	YAL061W	BDH2	""	33448	34701	1	Putative medium-chain alcohol dehydrogenase with similarity to BDH1	Putative medium-chain alcohol dehydrogenase with similarity to BDH1; transcription induced by constitutively active PDR1 and PDR3
S000000058	YAL062W	GDH3	Glutamate DeHydrogenase	31567	32940	1	NADP(+)-dependent glutamate dehydrogenase	NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh1p; expression regulated by nitrogen and carbon sources; GDH3 has a paralog, GDH1, that arose from the whole genome duplication
S000000061	YAL066W	""	""	10091	10399	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000000062	YAL067C	SEO1	Suppressor of sulfoxyde EthiOnine resistance	7235	9016	-1	Putative permease	Putative permease; member of the allantoate transporter subfamily of the major facilitator superfamily; mutation confers resistance to ethionine sulfoxide
S000000063	YAR002W	NUP60	NUclear Pore	152257	153876	1	FG-nucleoporin component of central core of the nuclear pore complex	FG-nucleoporin component of central core of the nuclear pore complex; contributes directly to nucleocytoplasmic transport and maintenance of the nuclear pore complex (NPC) permeability barrier; involved in gene tethering at the nuclear periphery; relocalizes to the cytosol in response to hypoxia; Cdc5p-mediated phosphorylation leads to detachment and reattachment of nuclear basket during meiosis; both NUP1 and NUP60 are homologous to human NUP153
S000000064	YAR003W	SWD1	Set1c, WD40 repeat protein	155005	156285	1	Subunit of the COMPASS (Set1C) complex	Subunit of the COMPASS (Set1C) complex; COMPASS methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member with similarity to mammalian Rbbp7
S000000065	YAR007C	RFA1	Replication Factor A	156754	158619	-1	Subunit of heterotrimeric Replication Protein A (RPA)	Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved ssDNA binding protein involved in DNA replication, repair, and recombination; RPA protects against inappropriate telomere recombination, and upon telomere uncapping, prevents cell proliferation by a checkpoint-independent pathway; promotes recruitment, helicase and nuclease activities of Dna2p; role in DNA catenation/decatenation pathway of chromosome disentangling; relocalizes to the cytosol in response to hypoxia
S000000066	YAR008W	SEN34	Splicing ENdonuclease	158966	159793	1	Subunit of the tRNA splicing endonuclease	Subunit of the tRNA splicing endonuclease; tRNA splicing endonuclease (Sen complex) is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen complex also cleaves the CBP1 mRNA at the mitochondrial surface; Sen34p contains the active site for tRNA 3' splice site cleavage and has similarity to Sen2p and to Archaeal tRNA splicing endonuclease
S000000069	YAR014C	BUD14	BUD site selection	166742	168871	-1	Protein involved in bud-site selection	Protein involved in bud-site selection; Bud14p-Glc7p complex is a cortical regulator of dynein; forms a complex with Kel1p and Kel2p that regulates Bnr1p (formin) to affect actin cable assembly, cytokinesis, and polarized growth; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; relative distribution to the nucleus increases upon DNA replication stress
S000000070	YAR015W	ADE1	ADEnine requiring	169375	170295	1	N-succinyl-5-aminoimidazole-4-carboxamide ribotide synthetase	N-succinyl-5-aminoimidazole-4-carboxamide ribotide synthetase; required for 'de novo' purine nucleotide biosynthesis; red pigment accumulates in mutant cells deprived of adenine; protein abundance increases in response to DNA replication stress
S000000071	YAR018C	KIN3	protein KINase	170396	171703	-1	Nonessential serine/threonine protein kinase	Nonessential serine/threonine protein kinase; possible role in DNA damage response; influences tolerance to high levels of ethanol
S000000072	YAR019C	CDC15	Cell Division Cycle	172211	175135	-1	Hippo-like kinase of the Mitotic Exit Network	Hippo-like kinase of the Mitotic Exit Network; promotes exit by activating the Dbf2p kinase; component of a non-canonical Hippo pathway with Sps1p required for prospore membrane closure, spindle disassembly and sustained release of Cdc14p during meiotic anaphase II; complexes with Sps1p and contributes to its phosphorylation; phosphorylates the RNAPII CTD during mitosis; localizes to the bud neck and SPB during anaphase and telophase; relocalizes to the cytoplasm upon DNA replication stress
S000000073	YAR020C	PAU7	seriPAUperin family	176856	177023	-1	Member of the seripauperin multigene family	Member of the seripauperin multigene family; active during alcoholic fermentation, regulated by anaerobiosis, inhibited by oxygen, repressed by heme
S000000074	YAR023C	DFP1	DUP240 Family Protein	179281	179820	-1	Putative integral membrane protein	Putative integral membrane protein; member of DUP240 gene family
S000000075	YAR027W	UIP3	Ulp1 Interacting Protein	183770	184477	1	Putative integral membrane protein of unknown function	Putative integral membrane protein of unknown function; interacts with Ulp1p at the nuclear periphery; member of DUP240 gene family
S000000076	YAR028W	KTD1	Killer Toxin Defens	184892	185596	1	Killer toxin defense factor	Killer toxin defense factor; key polymorphic factor in the defense against K28 toxin; localizes mainly to vacuolar membrane; most likely acts against K28 during its trafficking after endocytosis; member of DUP240 gene family
S000000077	YAR029W	DFP2	DUP240 Family Protein	186321	186545	1	Member of DUP240 gene family but contains no transmembrane domains	Member of DUP240 gene family but contains no transmembrane domains; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
S000000078	YAR031W	PRM9	Pheromone-Regulated Membrane protein	186836	187732	1	Pheromone-regulated protein	Pheromone-regulated protein; contains 3 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; member of DUP240 gene family; PRM9 has a paralog, PRM8, that arose from a segmental duplication
S000000080	YAR035W	YAT1	""	190193	192256	1	Outer mitochondrial carnitine acetyltransferase	Outer mitochondrial carnitine acetyltransferase; minor ethanol-inducible enzyme involved in transport of activated acyl groups from the cytoplasm into the mitochondrial matrix; phosphorylated
S000000081	YAR042W	SWH1	""	192619	196185	1	Protein similar to mammalian oxysterol-binding protein	Protein similar to mammalian oxysterol-binding protein; contains ankyrin repeats and FFAT motif; interacts with ER anchor Scs2p at the nucleus-vacuole junction; regulated by sterol binding; SWH1 has a paralog, OSH2, that arose from the whole genome duplication
S000000082	YAR044W	""	""	193600	196179	1	Merged open reading frame	Merged open reading frame; does not encode a discrete protein; YAR044W was originally annotated as an independent ORF, but as a result of a sequence change, it was merged with an adjacent ORF into a single reading frame, designated YAR042W
S000000083	YAR047C	""	""	201467	201787	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000000084	YAR050W	FLO1	FLOcculation	203403	208016	1	Lectin-like protein involved in flocculation	Lectin-like protein involved in flocculation; cell wall protein that binds mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin sensitive and heat resistant; important for co-flocculation with other yeasts, mediating interaction with specific species; FLO1 has a paralog, FLO5, that arose from a segmental duplication
S000000097	YBL001C	ECM15	ExtraCellular Mutant	237153	237467	-1	Non-essential protein of unknown function	Non-essential protein of unknown function; likely exists as tetramer, may be regulated by the binding of small-molecule ligands (possibly sulfate ions), may have a role in yeast cell-wall biogenesis
S000000098	YBL002W	HTB2	Histone h Two B	236492	236887	1	Histone H2B	Histone H2B; core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB1; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates reassembly after DNA replication, transcriptional activation, meiotic DSB formation and H3 methylation
S000000099	YBL003C	HTA2	Histone h Two A	235394	235792	-1	Histone H2A	Histone H2A; core histone protein required for chromatin assembly and chromosome function; one of two nearly identical (see also HTA1) subtypes; DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p
S000000100	YBL004W	UTP20	U Three Protein	227636	235117	1	Component of the small-subunit (SSU) processome	Component of the small-subunit (SSU) processome; SSU processome is involved in the biogenesis of the 18S rRNA
S000000101	YBL005W	PDR3	Pleiotropic Drug Resistance	217470	220400	1	Transcriptional activator of the pleiotropic drug resistance network	Transcriptional activator of the pleiotropic drug resistance network; regulates expression of ATP-binding cassette (ABC) transporters through binding to cis-acting PDRE sites (PDR responsive elements); has a role in response to drugs and organic solvents; post-translationally up-regulated in cells lacking functional mitochondrial genome; involved in diauxic shift; relative distribution to nucleus increases upon DNA replication stress; APCC(Cdh1) substrate
S000000102	YBL006C	LDB7	Low Dye Binding	216587	217129	-1	Component of the RSC chromatin remodeling complex	Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions
S000000103	YBL007C	SLA1	Synthetic Lethal with ABP1	212632	216366	-1	Cytoskeletal protein binding protein	Cytoskeletal protein binding protein; required for assembly of the cortical actin cytoskeleton; interacts with proteins regulating actin dynamics and proteins required for endocytosis; found in the nucleus and cell cortex; has 3 SH3 domains
S000000104	YBL008W	HIR1	HIstone Regulation	209653	212175	1	Subunit of the HIR complex	Subunit of the HIR complex; HIR is a nucleosome assembly complex involved in regulation of histone gene transcription; contributes to nucleosome formation, heterochromatic gene silencing, and formation of functional kinetochores
S000000105	YBL009W	ALK2	""	207194	209224	1	Atypical protein kinase	Atypical protein kinase; along with its paralog, ALK1, required for proper spindle positioning and nuclear segregation following mitotic arrest, proper organization of formins and polarisome components in mitosis, and survival after spindle assembly checkpoint delay; negatively regulates the morphogenesis checkpoint in response to polarity insults in opposition to ALK1; phosphorylated in response to DNA damage; similar to mammalian haspins
S000000106	YBL010C	LAA2	Large Adaptin Accessory	206107	206949	-1	Clathrin adaptor (adaptin) accessory protein	Clathrin adaptor (adaptin) accessory protein; physically interacts with and is involved in localization of clathrin adaptor protein complex-1 (AP-1); green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles
S000000107	YBL011W	SCT1	Suppressor of Choline-Transport mutants	203538	205817	1	Glycerol 3-phosphate/dihydroxyacetone phosphate sn-1 acyltransferase	Glycerol 3-phosphate/dihydroxyacetone phosphate sn-1 acyltransferase; dual substrate-specific acyltransferase of the glycerolipid biosynthesis pathway; prefers 16-carbon fatty acids; similar to Gpt2p; gene is constitutively transcribed
S000000108	YBL012C	""	""	203406	203807	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000000109	YBL013W	FMT1	Formyl-Methionyl-tRNA Transformylase	202056	203261	1	Methionyl-tRNA formyltransferase	Methionyl-tRNA formyltransferase; catalyzes formylation of mitochondria initiator Met-tRNA and residual cytosolic Met-tRNA due to inefficient translocation into the mitochondrial matrix; starvation or stationary phase entry increases Gcn2p phosphorylation-mediated cytosolic retention of Fmt1p, enhancing N-terminal protein formylation and ultimately N-end rule pathway mediated destruction that involves the Psh1p ubiquitin ligase; potential Cdc28p substrate
S000000110	YBL014C	RRN6	Regulation of RNA polymerase I	199064	201748	-1	Component of the core factor (CF) rDNA transcription factor complex	Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p
S000000111	YBL015W	ACH1	Acetyl CoA Hydrolase	194122	195702	1	Protein with CoA transferase activity	Protein with CoA transferase activity; particularly for CoASH transfer from succinyl-CoA to acetate; has minor acetyl-CoA-hydrolase activity; phosphorylated; required for acetate utilization and for diploid pseudohyphal growth
S000000112	YBL016W	FUS3	cell FUSion	192451	193512	1	Mitogen-activated serine/threonine protein kinase involved in mating	Mitogen-activated serine/threonine protein kinase involved in mating; phosphoactivated by Ste7p; substrates include Ste12p, Far1p, Bni1p, Sst2p; inhibits invasive growth during mating by phosphorylating Tec1p, promoting its; inhibits recruitment of Ste5p, Cdc42p-mediated asymmetry and mating morphogenesis
S000000113	YBL017C	PEP1	carboxyPEPtidase Y-deficient	186844	191583	-1	Type I transmembrane sorting receptor for multiple vacuolar hydrolases	Type I transmembrane sorting receptor for multiple vacuolar hydrolases; plays role in post-ER quality control in the Golgi, involved in protein targeting from the endoplasmic reticulum to the vacuole for degradation; cycles between late-Golgi and prevacuolar endosome-like compartments; targeted to vacuole via AP-3 pathway
S000000114	YBL018C	POP8	Processing Of Precursor RNAs	185998	186474	-1	Subunit of both RNase MRP and nuclear RNase P	Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; relocalizes to the cytosol in response to hypoxia
S000000115	YBL019W	APN2	APurinic/apyrimidinic eNdonuclease	184353	185915	1	Class II abasic (AP) endonuclease involved in repair of DNA damage	Class II abasic (AP) endonuclease involved in repair of DNA damage; also possesses 3'-diesterase and PCNA-stiimulated 3' to 5' exonuclease activities; resolves blocked 3' ends that result from removal of misincorporated nucleotides; frays and cleaves 3' DNA termini facilitating 3' DNA-end healing via endonucleolytic cleavage;  homolog of human APEX2 and E. coli exoIII 
S000000116	YBL020W	RFT1	Requiring Fifty-Three	182401	184125	1	Membrane protein required for translocation of Man5GlcNac2-PP-Dol	Membrane protein required for translocation of Man5GlcNac2-PP-Dol; required for translocation of Man5GlcNac2-PP-Dol from the cytoplasmic side to the lumenal side of the ER membrane but is not the flippase; mutation is suppressed by expression of human p53 protein; essential gene
S000000117	YBL021C	HAP3	Heme Activator Protein	181660	182094	-1	Subunit of the Hap2p/3p/4p/5p CCAAT-binding complex	Subunit of the Hap2p/3p/4p/5p CCAAT-binding complex; complex is heme-activated and glucose-repressed; complex is a transcriptional activator and global regulator of respiratory gene expression; contains sequences contributing to both complex assembly and DNA binding
S000000118	YBL022C	PIM1	Proteolysis In Mitochondria	177874	181275	-1	ATP-dependent Lon protease	ATP-dependent Lon protease; involved in degradation of misfolded mitochondrial proteins; required for mitochondrial maintenance and biogenesis; regulates mitochondrial DNA copy number with Mrx6p; subunit of a complex containing Mrx6p, Pet20p, and Mam33p that may regulate mtDNA replication; protease-independent, chaperone-like function in mitochondrial membrane complex assembly; localizes to the mitochondrial matrix
S000000119	YBL023C	MCM2	MiniChromosome Maintenance	174920	177526	-1	Protein involved in DNA replication	Protein involved in DNA replication; component of the Mcm2-7 hexameric helicase complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress
S000000120	YBL024W	NCL1	NuCLear protein	172534	174588	1	S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase	S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase; methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120
S000000121	YBL025W	RRN10	Regulation of RNA polymerase I	171481	171918	1	Protein involved in promoting high level transcription of rDNA	Protein involved in promoting high level transcription of rDNA; subunit of UAF (upstream activation factor) for RNA polymerase I
S000000123	YBL027W	RPL19B	Ribosomal Protein of the Large subunit	168423	169376	1	Ribosomal 60S subunit protein L19B	Ribosomal 60S subunit protein L19B; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19B has a paralog, RPL19A, that arose from the whole genome duplication
S000000124	YBL028C	""	""	167518	167838	-1	Protein of unknown function that may interact with ribosomes	Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis
S000000125	YBL029W	""	""	166134	167264	1	Non-essential protein of unknown function	Non-essential protein of unknown function
S000000126	YBL030C	PET9	PETite	163041	163997	-1	Major ADP/ATP carrier of the mitochondrial inner membrane	Major ADP/ATP carrier of the mitochondrial inner membrane; exchanges cytosolic ADP for mitochondrially synthesized ATP; also imports heme and ATP; also functions in peroxisomes; required for viability in many lab strains that carry a sal1 mutation; PET9 has a paralog, AAC3, that arose from the whole genome duplication; human homolog SLC25A4 implicated in progressive external ophthalmoplegia can complement yeast null mutant
S000000127	YBL031W	SHE1	Sensitivity to High Expression	161699	162715	1	Mitotic spindle protein	Mitotic spindle protein; interacts with components of the Dam1 (DASH) complex, its effector Sli15p, and microtubule-associated protein Bim1p; also localizes to nuclear microtubules and to the bud neck in a ring-shaped structure; inhibits dynein function
S000000128	YBL032W	HEK2	HEterogeneous nuclear rnp K-like gene	160184	161329	1	RNA binding protein involved in asymmetric localization of ASH1 mRNA	RNA binding protein involved in asymmetric localization of ASH1 mRNA; represses translation of ASH1 mRNA, an effect reversed by Yck1p-dependent phosphoryation; regulates telomere position effect and length; similarity to hnRNP-K; increases mRNA stability by binding (CNN)n repeat elements
S000000129	YBL033C	RIB1	RIBoflavin biosynthesis	158656	159693	-1	GTP cyclohydrolase II	GTP cyclohydrolase II; catalyzes the first step of the riboflavin biosynthesis pathway
S000000131	YBL035C	POL12	POLymerase	151493	153610	-1	B subunit of DNA polymerase alpha-primase complex	B subunit of DNA polymerase alpha-primase complex; required for initiation of DNA replication during mitotic and premeiotic DNA synthesis; also functions in telomere capping and length regulation
S000000132	YBL036C	""	""	150447	151220	-1	Putative non-specific single-domain racemase	Putative non-specific single-domain racemase; based on structural similarity; binds pyridoxal 5'-phosphate; expression of GFP-fusion protein induced in response to the DNA-damaging agent MMS
S000000133	YBL037W	APL3	clathrin Adaptor Protein complex Large chain	147209	150286	1	Alpha-adaptin	Alpha-adaptin; large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport
S000000134	YBL038W	MRPL16	Mitochondrial Ribosomal Protein, Large subunit	146187	146885	1	Mitochondrial ribosomal protein of the large subunit	Mitochondrial ribosomal protein of the large subunit; homologous to bacterial L16 ribosomal protein; synthetic lethality with hac1 mutation suggests a possible role in synthesis of precursors for protein glycosylation
S000000135	YBL039C	URA7	URAcil requiring	143989	145728	-1	Major CTP synthase isozyme (see also URA8)	Major CTP synthase isozyme (see also URA8); catalyzes ATP-dependent transfer of amide nitrogen from glutamine to UTP, forming CTP, final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; targeted to vacuole via AP-3 pathway; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA7 has paralog URA8 that arose from the whole genome duplication
S000000137	YBL041W	PRE7	PRoteinase yscE	141247	141972	1	Beta 6 subunit of the 20S proteasome	Beta 6 subunit of the 20S proteasome
S000000138	YBL042C	FUI1	5-FlUorourIdine resistance	138341	140260	-1	High affinity uridine permease, localizes to the plasma membrane	High affinity uridine permease, localizes to the plasma membrane; also mediates low but significant transport of the cytotoxic nucleoside analog 5-fluorouridine; not involved in uracil transport; relative distribution to the vacuole increases upon DNA replication stress
S000000139	YBL043W	ECM13	ExtraCellular Mutant	136688	137461	1	Non-essential protein of unknown function	Non-essential protein of unknown function; induced by treatment with 8-methoxypsoralen and UVA irradiation; ECM13 has a paralog, YJR115W, that arose from the whole genome duplication
S000000140	YBL044W	""	""	135998	136366	1	Putative protein of unknown function	Putative protein of unknown function; YBL044W is not an essential protein
S000000141	YBL045C	COR1	CORe protein of QH2 cytochrome c reductase	134143	135516	-1	Core subunit of the ubiquinol-cytochrome c reductase complex	Core subunit of the ubiquinol-cytochrome c reductase complex; the ubiquinol-cytochrome c reductase complex (bc1 complex) is a component of the mitochondrial inner membrane electron transport chain
S000000142	YBL046W	PSY4	Platinum SensitivitY	132424	133749	1	Regulatory subunit of protein phosphatase PP4	Regulatory subunit of protein phosphatase PP4; presence of Psy4p in the PP4 complex (along with catalytic subunit Pph3p and Psy2p) is required for dephosphorylation of the histone variant H2AX, but not for dephosphorylation of Rad53p, during recovery from the DNA damage checkpoint; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; required for cisplatin resistance; homolog of mammalian R2
S000000143	YBL047C	EDE1	EH Domains and Endocytosis	127895	132040	-1	Endocytic adaptor	Endocytic adaptor; K63-specific Ub-chain binding protein involved in receptor endocytosis; scaffold protein that localizes to nascent endocytic sites after homo-oligomerization and organizes the endocytic machinery; role in autophagy of aberrant clathrin-mediated endocytosis protein condensates; binds membranes in a Ub-dependent manner; interacts with and functions upstream of Cmk2p to suppress stress-induced non-apoptotic cell death; putative cytokinesis regulator;  homolog of mammalian Eps15
S000000144	YBL048W	RRT1	Regulator of rDNA Transcription	127299	127610	1	Putative protein of unknown function	Putative protein of unknown function; conserved across S. cerevisiae strains; identified in a screen for mutants with increased levels of rDNA transcription
S000000145	YBL049W	MOH1	""	126828	127244	1	Protein of unknown function, essential for stationary phase survival	Protein of unknown function, essential for stationary phase survival; not required for growth on nonfermentable carbon sources; possibly linked with vacuolar transport
S000000146	YBL050W	SEC17	SECretory	125125	126119	1	Alpha-SNAP cochaperone	Alpha-SNAP cochaperone; SNARE-complex adaptor for Sec18 (NSF) during the disassembly of postfusion cis-SNARE complexes; stimulates the ATPase activity of Sec18p; peripheral membrane protein required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, and autophagy; similar to mammalian alpha-SNAP
S000000147	YBL051C	PIN4	Psi+ INducibility	122753	124759	-1	Protein involved in G2/M phase progression and response to DNA damage	Protein involved in G2/M phase progression and response to DNA damage; interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage
S000000148	YBL052C	SAS3	Something About Silencing	119379	121874	-1	Histone acetyltransferase catalytic subunit of NuA3 complex	Histone acetyltransferase catalytic subunit of NuA3 complex; acetylates histone H3, involved in transcriptional silencing; homolog of the mammalian MOZ proto-oncogene; mutant has aneuploidy tolerance; sas3gcn5 double mutation is lethal
S000000149	YBL053W	""	""	119335	119709	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000000150	YBL054W	TOD6	Twin Of Dot6p	117589	119166	1	PAC motif binding protein involved in rRNA and ribosome biogenesis	PAC motif binding protein involved in rRNA and ribosome biogenesis; subunit of the RPD3L histone deacetylase complex; Myb-like HTH transcription factor; hypophosphorylated by rapamycin treatment in a Sch9p-dependent manner; activated in stochastic pulses of nuclear localization
S000000151	YBL055C	""	""	115573	116829	-1	3'-->5' exonuclease and endonuclease with a possible role in apoptosis	3'-->5' exonuclease and endonuclease with a possible role in apoptosis; has similarity to mammalian and C. elegans apoptotic nucleases
S000000152	YBL056W	PTC3	Phosphatase Two C	113762	115168	1	Type 2C protein phosphatase (PP2C)	Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p (see also Ptc2p) to limit maximal kinase activity induced by osmotic stress; dephosphorylates T169 phosphorylated Cdc28p (see also Ptc2p); role in DNA damage checkpoint inactivation; PTC3 has a paralog, PTC2, that arose from the whole genome duplication
S000000153	YBL057C	PTH2	Peptidyl-Trna Hydrolase	112801	113427	-1	One of two mitochondrially-localized peptidyl-tRNA hydrolases	One of two mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth; see also PTH1
S000000154	YBL058W	SHP1	Suppressor of High-copy PP1	111437	112708	1	Substrate adaptor for Cdc48p	Substrate adaptor for Cdc48p; ubiquitin regulatory X (UBX) and Ub-associated (UBA) domain-containing protein that acts as a substrate recruiting cofactor; K48-specific Ub chain binding protein involved in ER-associated degradation (ERAD); positively regulates Glc7p protein phosphatase activity to promote growth and mitotic progression in complex with Cdc48p; regulated by nuclear Ub-dependent degradation (INMAD pathway) independent of the Asi and Doa10 complexes; homolog of human p47 (NSFL1C)
S000000155	YBL059W	IAI11	Interactor of AIm11	110594	111244	1	Putative mitochondrial protein of unknown function	Putative mitochondrial protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; IAI11 has a paralog, YER093C-A, that arose from the whole genome duplication
S000000156	YBL060W	YEL1	Yeast EFA6-Like	107932	109995	1	Guanine nucleotide exchange factor specific for Arf3p	Guanine nucleotide exchange factor specific for Arf3p; localized to the bud neck and tip; required for localization of Arf3p to the bud neck and tip
S000000157	YBL061C	SKT5	""	105316	107406	-1	Activator of Chs3p (chitin synthase III) during vegetative growth	Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication
S000000158	YBL062W	""	""	105308	105688	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000000159	YBL063W	KIP1	KInesin related Protein	101886	105221	1	Kinesin-related motor protein	Kinesin-related motor protein; tracks plus-ends and stabilizes microtubules; required for mitotic spindle assembly, chromosome segregation, and 2 micron plasmid partitioning; functionally redundant with Cin8p for chromosomal but not plasmid functions
S000000160	YBL064C	PRX1	PeroxiRedoXin	100371	101156	-1	Mitochondrial peroxiredoxin with thioredoxin peroxidase activity	Mitochondrial peroxiredoxin with thioredoxin peroxidase activity; has a role in reduction of hydroperoxides; reactivation requires Trr2p and glutathione; induced during respiratory growth and oxidative stress; phosphorylated; protein abundance increases in response to DNA replication stress
S000000161	YBL065W	""	""	99963	100307	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified ORF SEF1/YBL066C; YBL065W is a non-essential gene
S000000162	YBL066C	SEF1	Suppressor of Essential Function	96669	100115	-1	Putative transcription factor	Putative transcription factor; has homolog in Kluyveromyces lactis
S000000163	YBL067C	UBP13	UBiquitin-specific Protease	93639	95882	-1	Ubiquitin-specific protease that cleaves Ub-protein fusions	Ubiquitin-specific protease that cleaves Ub-protein fusions; UBP13 has a paralog, UBP9, that arose from the whole genome duplication
S000000164	YBL068W	PRS4	PhosphoRibosylpyrophosphate Synthetase	92414	93394	1	5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP	5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP; which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS4 has a paralog, PRS2, that arose from the whole genome duplication; a missense mutation in the conserved residue R196 of its human homolog PRPS1 is pathogenic
S000000165	YBL069W	AST1	ATPase STabilizing	90741	92030	1	Lipid raft associated protein	Lipid raft associated protein; interacts with the plasma membrane ATPase Pma1p and has a role in its targeting to the plasma membrane by influencing its incorporation into lipid rafts; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST1 has a paralog, AST2, that arose from the whole genome duplication
S000000166	YBL070C	""	""	90605	90925	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000000167	YBL071C	""	""	90223	90531	-1	Putative protein of unknown function	Putative protein of unknown function; conserved among S. cerevisiae strains; YBL071C is not an essential gene
S000000168	YBL072C	RPS8A	Ribosomal Protein of the Small subunit	88523	89125	-1	Protein component of the small (40S) ribosomal subunit	Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S8, no bacterial homolog; RPS8A has a paralog, RPS8B, that arose from the whole genome duplication
S000000169	YBL073W	""	""	87646	87957	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the essential gene AAR2/YBL074C
S000000170	YBL074C	AAR2	A1-Alpha2 Repression	86722	87789	-1	Component of the U5 snRNP complex	Component of the U5 snRNP complex; required for splicing of U3 precursors; originally described as a splicing factor specifically required for splicing pre-mRNA of the MATa1 cistron
S000000171	YBL075C	SSA3	Stress-Seventy subfamily A	84499	86448	-1	ATPase involved in protein folding and the response to stress	ATPase involved in protein folding and the response to stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the heat shock protein 70 (HSP70) family; localized to the cytoplasm; SSA3 has a paralog, SSA4, that arose from the whole genome duplication
S000000172	YBL076C	ILS1	IsoLeucine-tRNA Synthetase	81043	84261	-1	Cytoplasmic isoleucine-tRNA synthetase	Cytoplasmic isoleucine-tRNA synthetase; target of the G1-specific inhibitor reveromycin A
S000000174	YBL078C	ATG8	AuTophaGy related	80378	80731	-1	Ubiquitin-like protein conjugated to phosphatidylethanolamine (PE)	Ubiquitin-like protein conjugated to phosphatidylethanolamine (PE); role in membrane fusion and phagophore expansion during autophagosome formation; deconjugation is also required for biogenesis; targets Atg1p to autophagosomes; binds to the Atg1p-Atg13p complex, triggers its vacuolar degradation; role in vacuolar membrane protein turnover during early stationary phase; component of autophagosomes and Cvt vesicles; targeted to vacuole via AP-3 pathway
S000000175	YBL079W	NUP170	NUclear Pore	75259	79767	1	Subunit of inner ring of nuclear pore complex (NPC)	Subunit of inner ring of nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; interacts with genomic regions that contain ribosomal protein and subtelomeric genes, where it functions in nucleosome positioning and as a repressor of transcription; both Nup170p and Nup157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication
S000000176	YBL080C	PET112	PETite colonies	73070	74695	-1	Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex	Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; mutation is functionally complemented by the bacterial GatB ortholog
S000000177	YBL081W	""	""	71866	72972	1	Non-essential protein of unknown function	Non-essential protein of unknown function; null mutation results in a decrease in plasma membrane electron transport
S000000178	YBL082C	ALG3	Asparagine Linked Glycosylation	69751	71127	-1	Dolichol-P-Man dependent alpha(1-3) mannosyltransferase	Dolichol-P-Man dependent alpha(1-3) mannosyltransferase; involved in synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins; G353A missense mutation in human ortholog ALG3 implicated in carbohydrate deficient glycoprotein syndrome type IV, which is characterized by microcephaly, severe epilepsy, minimal psychomotor development, partial deficiency of sialic acids in serum glycoproteins; wild-type human ALG3 can complement yeast alg3 mutant
S000000179	YBL083C	""	""	69713	70138	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3
S000000180	YBL084C	CDC27	Cell Division Cycle	67169	69445	-1	Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C)	Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
S000000181	YBL085W	BOI1	Bem1 (One) Interacting protein	63876	66818	1	Protein involved in polar growth, functionally redundant with Boi2p	Protein involved in polar growth, functionally redundant with Boi2p; required for fusion of secretory vesicles with plasma membrane at sites of polarized growth; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication
S000000182	YBL086C	""	""	61202	62602	-1	Protein of unknown function	Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
S000000183	YBL087C	RPL23A	Ribosomal Protein of the Large subunit	59822	60739	-1	Ribosomal 60S subunit protein L23A	Ribosomal 60S subunit protein L23A; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23A has a paralog, RPL23B, that arose from the whole genome duplication
S000000184	YBL088C	TEL1	TELomere maintenance	51019	59382	-1	Protein kinase primarily involved in telomere length regulation	Protein kinase primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; binds to phosphatidylinositol-4-phosphate; acts with Red1p and Mec1p to promote interhomolog recombination by phosphorylation of Hop1; functionally redundant with Mec1p; regulates P-body formation induced by replication stress; homolog of human ataxia-telangiectasia mutated (ATM) gene
S000000185	YBL089W	AVT5	Amino acid Vacuolar Transport	49574	50953	1	Putative transporter	Putative transporter; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters; AVT5 has a paralog, AVT6, that arose from the whole genome duplication
S000000186	YBL090W	MRP21	Mitochondrial Ribosomal Protein	48825	49358	1	Mitochondrial ribosomal protein of the small subunit	Mitochondrial ribosomal protein of the small subunit; MRP21 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences
S000000187	YBL091C	MAP2	Methionine AminoPeptidase	47363	48628	-1	Methionine aminopeptidase	Methionine aminopeptidase; catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map1p
S000000188	YBL092W	RPL32	Ribosomal Protein of the Large subunit	45978	46370	1	Ribosomal 60S subunit protein L32	Ribosomal 60S subunit protein L32; overexpression disrupts telomeric silencing; homologous to mammalian ribosomal protein L32, no bacterial homolog
S000000189	YBL093C	ROX3	Regulation by OXygen	44256	44918	-1	Subunit of the RNA polymerase II mediator complex	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme
S000000190	YBL094C	""	""	43765	44097	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YBL095W
S000000191	YBL095W	MRX3	Mitochondrial oRganization of gene eXpression (MIOREX)	43277	44089	1	Protein that associates with mitochondrial ribosome	Protein that associates with mitochondrial ribosome; likely functions in cristae junction formation; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
S000000192	YBL096C	""	""	43174	43482	-1	Non-essential protein of unknown function	Non-essential protein of unknown function
S000000193	YBL097W	BRN1	homolog of BaRreN	40831	43095	1	Subunit of the condensin complex	Subunit of the condensin complex; required for chromosome condensation and for clustering of tRNA genes at the nucleolus; may influence multiple aspects of chromosome transmission
S000000194	YBL098W	BNA4	Biosynthesis of Nicotinic Acid	39145	40527	1	Kynurenine 3-monooxygenase	Kynurenine 3-monooxygenase; required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p; localizes to mitochondria and peroxisomes; putative therapeutic target for Huntington disease
S000000195	YBL099W	ATP1	ATP synthase	37053	38690	1	Alpha subunit of the F1 sector of mitochondrial F1F0 ATP synthase	Alpha subunit of the F1 sector of mitochondrial F1F0 ATP synthase; which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; F1 translationally regulates ATP6 and ATP8 expression to achieve a balanced output of ATP synthase genes encoded in nucleus and mitochondria; phosphorylated; N-terminally propionylated in vivo
S000000196	YBL100C	""	""	36989	37303	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps the 5' end of ATP1
S000000197	YBL101C	ECM21	ExtraCellular Mutant	24946	28299	-1	Alpha-arrestin, ubiquitin ligase adaptor for Rsp5p	Alpha-arrestin, ubiquitin ligase adaptor for Rsp5p; regulates starvation- and substrate-induced Ub-dependent endocytosis of select plasma membrane localized amino acid transporters; substrate of Rsp5p-mediated Lys63-polyubiquitination and Ubp2-mediated deubiquitination; promoter contains several Gcn4p binding sites required for induction in response to starvation via Gcn4p and the general amino acid control pathway; ECM21 has a paralog, CSR2, that arose from the whole genome duplication
S000000198	YBL102W	SFT2	Suppressor of sed Five Ts	24098	24745	1	Tetra-spanning membrane protein found mostly in the late Golgi	Tetra-spanning membrane protein found mostly in the late Golgi; non-essential; can suppress some sed5 alleles; may be part of the transport machinery, but precise function is unknown; similar to mammalian syntaxin 5
S000000199	YBL103C	RTG3	ReTroGrade regulation	22076	23536	-1	bHLH/Zip transcription factor for retrograde (RTG) and TOR pathways	bHLH/Zip transcription factor for retrograde (RTG) and TOR pathways; forms a complex with another bHLH/Zip protein, Rtg1p, to activate the pathways; target of Hog1p
S000000200	YBL104C	SEA4	SEh1-Associated	18177	21293	-1	Subunit of SEACAT, a subcomplex of the SEA complex	Subunit of SEACAT, a subcomplex of the SEA complex; Sea4p, along with Rtc1p and Mtc5p, redundantly inhibit the TORC1 inhibitory role of the Iml1p/SEACIT (Iml1p-Npr2p-Npr3p) subcomplex, a GAP for GTPase Gtr1p (EGOC subunit) in response to amino acid limitation, thereby resulting in activation of TORC1 signaling; SEA is a coatomer-related complex that associates dynamically with the vacuole; contains an N-terminal beta-propeller fold and a C-terminal RING motif
S000000201	YBL105C	PKC1	Protein Kinase C	14241	17696	-1	Protein serine/threonine kinase	Protein serine/threonine kinase; essential for cell wall remodeling during growth; localized to sites of polarized growth and the mother-daughter bud neck; homolog of the alpha, beta, and gamma isoforms of mammalian protein kinase C (PKC)
S000000202	YBL106C	SRO77	Suppressor of rho3	10847	13879	-1	Protein with roles in exocytosis and cation homeostasis	Protein with roles in exocytosis and cation homeostasis; functions in docking and fusion of post-Golgi vesicles with plasma membrane; regulates cell proliferation and colony development via the Rho1-Tor1 pathway; interacts with SNARE protein Sec9p; homolog of Drosophila lethal giant larvae tumor suppressor; SRO77 has a paralog, SRO7, that arose from the whole genome duplication
S000000203	YBL107C	MIX23	Mitochondrial Intermembrane space CX(n)C motif protein	9961	10551	-1	Mitochondrial intermembrane space protein of unknown function	Mitochondrial intermembrane space protein of unknown function; imported via the MIA import machinery; contains an unusual twin cysteine motif (CX13C CX14C)
S000000205	YBR001C	NTH2	Neutral TreHalase	238941	241283	-1	Putative neutral trehalase, required for thermotolerance	Putative neutral trehalase, required for thermotolerance; may mediate resistance to other cellular stresses; NTH2 has a paralog, NTH1, that arose from the whole genome duplication
S000000207	YBR003W	COQ1	COenzyme Q	242809	244230	1	Hexaprenyl pyrophosphate synthetase	Hexaprenyl pyrophosphate synthetase; catalyzes the first step in ubiquinone (coenzyme Q) biosynthesis
S000000208	YBR004C	GPI18	GlycosylPhosphatidylInositol	244366	245667	-1	Functional ortholog of human PIG-V	Functional ortholog of human PIG-V; PIG-V is a mannosyltransferase that transfers the second mannose in glycosylphosphatidylinositol biosynthesis; the authentic, non-tagged protein was localized to mitochondria
S000000209	YBR005W	RCR1	Resistance to Congo Red	245906	246547	1	Plasma membrane (PM) ubiquitin ligase-substrate adaptor	Plasma membrane (PM) ubiquitin ligase-substrate adaptor; involved in cell wall chitin deposition; ubiquitinates and down-regulates chitin synthase Chs3p abundance at PM via calcineurin/Crz1 signaling pathway which regulates RCR1 expression; delivered to PM by exomer complex using novel PM sorting motif; RCR1 has a paralog, RCR2, that arose from the whole genome duplication
S000000210	YBR006W	UGA2	Utilization of GAba	247010	248503	1	Succinate semialdehyde dehydrogenase	Succinate semialdehyde dehydrogenase; involved in the utilization of gamma-aminobutyrate (GABA) as a nitrogen source; part of the 4-aminobutyrate and glutamate degradation pathways; localized to the cytoplasm
S000000211	YBR007C	DSF2	Deletion Suppressor of mptFive/pufFive mutation	248806	251016	-1	Deletion suppressor of mpt5 mutation	Deletion suppressor of mpt5 mutation; relocalizes from bud neck to cytoplasm upon DNA replication stress
S000000212	YBR008C	FLR1	FLuconazole Resistance	252563	254209	-1	Plasma membrane transporter of the major facilitator superfamily	Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; involved in efflux of fluconazole, diazaborine, benomyl, methotrexate, and other drugs; expression induced in cells treated with the mycotoxin patulin; relocalizes from nucleus to plasma membrane upon DNA replication stress
S000000213	YBR009C	HHF1	Histone H Four	255373	255684	-1	Histone H4	Histone H4; core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF2); contributes to telomeric silencing; involved in mRNA multiplexing; N-terminal domain involved in maintaining genomic integrity
S000000214	YBR010W	HHT1	Histone H Three	256331	256741	1	Histone H3	Histone H3; core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT2); regulated by acetylation, methylation, and phosphorylation; H3K14 acetylation plays an important role in the unfolding of strongly positioned nucleosomes during repair of UV damage
S000000215	YBR011C	IPP1	Inorganic PyroPhosphatase	257112	257975	-1	Cytoplasmic inorganic pyrophosphatase (PPase)	Cytoplasmic inorganic pyrophosphatase (PPase); homodimer that catalyzes the rapid exchange of oxygens from Pi with water, highly expressed and essential for viability, active-site residues show identity to those from E. coli PPase
S000000216	YBR012C	""	""	259147	259566	-1	Dubious open reading frame	Dubious open reading frame; appears conserved and shows evidence of preservation by purifying selection within the Saccharomycotina subphylum; expression induced by iron-regulated transcriptional activator Aft2p
S000000217	YBR013C	""	""	265492	265881	-1	Putative protein of unknown function	Putative protein of unknown function; haploid deletion mutant exhibits synthetic phenotype with alpha-synuclein; SWAT-GFP fusion protein localizes to the endoplasmic reticulum while mCherry fusion protein localizes to both the endoplasmic reticulum and vacuole
S000000218	YBR014C	GRX7	GlutaRedoXin	266725	267336	-1	Cis-golgi localized monothiol glutaredoxin	Cis-golgi localized monothiol glutaredoxin; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; does not bind metal ions; GRX7 has a paralog, GRX6, that arose from the whole genome duplication
S000000219	YBR015C	MNN2	MaNNosyltransferase	267710	269503	-1	Alpha-1,2-mannosyltransferase	Alpha-1,2-mannosyltransferase; responsible for addition of the first alpha-1,2-linked mannose to form the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment
S000000220	YBR016W	""	""	270247	270633	1	Tail-anchored plasma membrane protein with a conserved CYSTM module	Tail-anchored plasma membrane protein with a conserved CYSTM module; predicted to be palmitoylated; has similarity to hydrophilins, which are involved in the adaptive response to hyperosmotic conditions; YBR016W has a paralog, YDL012C, that arose from the whole genome duplication
S000000222	YBR018C	GAL7	GALactose metabolism	274427	275527	-1	Galactose-1-phosphate uridyl transferase	Galactose-1-phosphate uridyl transferase; synthesizes glucose-1-phosphate and UDP-galactose from UDP-D-glucose and alpha-D-galactose-1-phosphate in the second step of galactose catabolism; human homolog UGP2 can complement yeast null mutant
S000000223	YBR019C	GAL10	GALactose metabolism	276253	278352	-1	UDP-glucose-4-epimerase	UDP-glucose-4-epimerase; catalyzes interconversion of UDP-galactose and UDP-D-glucose in galactose metabolism; also catalyzes conversion of alpha-D-glucose or alpha-D-galactose to their beta-anomers; human homolog GALE implicated in galactosemia, can complement yeast null mutant
S000000224	YBR020W	GAL1	GALactose metabolism	279021	280607	1	Galactokinase	Galactokinase; phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p; human homolog GALK2 complements yeast null mutant; GAL1 has a paralog, GAL3, that arose from the whole genome duplication
S000000225	YBR021W	FUR4	5-FlUoRouridine sensitivity	281443	283344	1	Plasma membrane localized uracil permease	Plasma membrane localized uracil permease; expression is tightly regulated by uracil levels and environmental cues; conformational alterations induced by unfolding or substrate binding result in Rsp5p-mediated ubiquitination and degradation
S000000226	YBR022W	POA1	Phosphatase Of ADP-ribose 1"-phosphate	283738	284271	1	Phosphatase that is highly specific for ADP-ribose 1''-phosphate	Phosphatase that is highly specific for ADP-ribose 1''-phosphate; a tRNA splicing metabolite; may have a role in regulation of tRNA splicing
S000000227	YBR023C	CHS3	CHitin Synthase-related	284428	287925	-1	Chitin synthase III	Chitin synthase III; catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan; contains overlapping di-leucine and di-acidic signals that mediate, respectively, intracellular trafficking by AP-1 and trafficking to plasma membrane by exomer complex; requires AP-3 complex for its intracellular retention
S000000228	YBR024W	SCO2	Suppressor of Cytochrome Oxidase deficiency	289445	290350	1	Protein anchored to mitochondrial inner membrane	Protein anchored to mitochondrial inner membrane; may have a redundant function with Sco1p in delivery of copper to cytochrome c oxidase; interacts with Cox2p; SCO2 has a paralog, SCO1, that arose from the whole genome duplication
S000000229	YBR025C	OLA1	Obg-Like ATPase	290681	291865	-1	P-loop ATPase with similarity to human OLA1 and bacterial YchF	P-loop ATPase with similarity to human OLA1 and bacterial YchF; identified as specifically interacting with the proteasome; positively regulates heat shock response; null mutant displays increased translation rate and increased readthrough of premature stop codons; protein abundance increases in response to hydrogen peroxide and to DNA replication stress
S000000230	YBR026C	ETR1	2-Enoyl Thioester Reductase	292877	294019	-1	2-enoyl thioester reductase	2-enoyl thioester reductase; member of the medium chain dehydrogenase/reductase family; localized to mitochondria, where it has a probable role in fatty acid synthesis; human MECR functionally complements the respiratory growth defect of the null mutant
S000000231	YBR027C	""	""	294024	294356	-1	Putative protein of unknown function	Putative protein of unknown function; conserved among S. cerevisiae strains; YBR027C is not an essential gene
S000000232	YBR028C	YPK3	""	294425	296002	-1	AGC kinase	AGC kinase; phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner; directly phosphorylated by TORC1; phosphorylates ribosomal protein Rps6a/b (S6), in a TORC-dependent manner; undergoes autophosphorylation
S000000233	YBR029C	CDS1	CDP-Diacylglycerol Synthase	296369	297742	-1	Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase)	Phosphatidate cytidylyltransferase (CDP-diglyceride synthetase); an enzyme that catalyzes that conversion of CTP + phosphate into diphosphate + CDP-diaclglyerol, a critical step in the synthesis of all major yeast phospholipids; human homolog CDS1 can complement yeast cds1 null mutant
S000000234	YBR030W	RKM3	Ribosomal lysine (K) Methyltransferase	298292	299950	1	Ribosomal lysine methyltransferase	Ribosomal lysine methyltransferase; specific for monomethylation of Rpl42ap and Rpl42bp (lysine 40); nuclear SET domain containing protein; relocalizes to the cytosol in response to hypoxia
S000000235	YBR031W	RPL4A	Ribosomal Protein of the Large subunit	300166	301254	1	Ribosomal 60S subunit protein L4A	Ribosomal 60S subunit protein L4A; N-terminally acetylated; homologous to mammalian ribosomal protein L4 and bacterial L4; RPL4A has a paralog, RPL4B, that arose from the whole genome duplication
S000000236	YBR032W	""	""	301519	301821	1	Putative protein of unknown function	Putative protein of unknown function; conserved among S. cerevisiae strains; YBR032W is not an essential gene
S000000237	YBR033W	EDS1	Expression Dependent on Slt2	301944	304703	1	Putative zinc cluster protein, predicted to be a transcription factor	Putative zinc cluster protein, predicted to be a transcription factor; not an essential gene; EDS1 has a paralog, RGT1, that arose from the whole genome duplication
S000000238	YBR034C	HMT1	HnRNP MethylTransferase	304930	305976	-1	Nuclear SAM-dependent mono- and asymmetric methyltransferase	Nuclear SAM-dependent mono- and asymmetric methyltransferase; modifies hnRNPs, including Npl3p and Hrp1p, affecting their activity and nuclear export; methylates U1 snRNP protein Snp1p, ribosomal protein Rps2p, and histones H3 and H4; interacts genetically with genes encoding components of Rpd3(L) and this interaction is important for Rpd3 recruitment to the subtelomeric region
S000000239	YBR035C	PDX3	PyriDoXine auxotrophy	306269	306955	-1	Pyridoxine (pyridoxamine) phosphate oxidase	Pyridoxine (pyridoxamine) phosphate oxidase; has homologs in E. coli and Myxococcus xanthus; transcription is under the general control of nitrogen metabolism
S000000240	YBR036C	CSG2	Calcium Sensitive Growth	309081	310313	-1	Calcium release channel involved in ER calcium homeostasis	Calcium release channel involved in ER calcium homeostasis; integrates ER calcium release with the regulation of sphingolipid metabolism and autophagy; required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; endoplasmic reticulum membrane protein; contains 10 transmembrane domains and a potential Ca2+ EF-hand motif; protein abundance increases in response to DNA replication stress
S000000241	YBR037C	SCO1	Suppressor of Cytochrome Oxidase deficiency	310565	311452	-1	Copper-binding protein of mitochondrial inner membrane	Copper-binding protein of mitochondrial inner membrane; required for cytochrome c oxidase activity and respiration; may function to deliver copper to cytochrome c oxidase; similar to thioredoxins; SCO1 has a paralog, SCO2, that arose from the whole genome duplication
S000000242	YBR038W	CHS2	CHitin Synthase	311898	314789	1	Chitin synthase II	Chitin synthase II; catalyzes transfer of N-acetylglucosamine (GlcNAc) to chitin upon activation of zymogenic form; required for chitin synthesis in the primary septum during cytokinesis; localization regulated by Cdk1p during mitosis; phosphorylation by Dbf2p kinase regulates its dynamics and chitin synthesis during cytokinesis
S000000244	YBR040W	FIG1	Factor-Induced Gene	316968	317864	1	Integral membrane protein required for efficient mating	Integral membrane protein required for efficient mating; may participate in or regulate the low affinity Ca2+ influx system, which affects intracellular signaling and cell-cell fusion during mating; targeted to vacuole via AP-3 pathway
S000000245	YBR041W	FAT1	FATty acid transporter	318266	320275	1	Very long chain fatty acyl-CoA synthetase and fatty acid transporter	Very long chain fatty acyl-CoA synthetase and fatty acid transporter; activates imported fatty acids with a preference for very long lengths (C20-C26); has a separate function in the transport of long chain fatty acids
S000000246	YBR042C	CST26	Chromosome STability	320416	321609	-1	Acyltransferase	Acyltransferase; enzyme mainly responsible for the introduction of saturated very long chain fatty acids into neo-synthesized molecules of phosphatidylinositol; required for incorporation of stearic acid into phosphatidylinositol; affects chromosome stability when overexpressed; CST26 has a paralog, YDR018C, that arose from the whole genome duplication
S000000247	YBR043C	QDR3	QuiniDine Resistance	321876	323945	-1	Multidrug transporter of the major facilitator superfamily	Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; has a role in polyamine homeostasis; involved in spore wall asembly; sequence similarity to DTR1 and QDR1, and the triple mutant dtr1 qdr1 qdr3 exhibits reduced dityrosine fluorescence relative to the single mutants; expression is upregulated under polyamine stress; required for resistance to quinidine, barban, cisplatin, and bleomycin
S000000248	YBR044C	TCM62	TriCarboxylic acid cycle Mutant	324341	326059	-1	Protein involved in assembly of the succinate dehydrogenase complex	Protein involved in assembly of the succinate dehydrogenase complex; mitochondrial; putative chaperone
S000000249	YBR045C	GIP1	Glc7-Interacting Protein	328173	330092	-1	Meiosis-specific regulatory subunit of the Glc7p protein phosphatase	Meiosis-specific regulatory subunit of the Glc7p protein phosphatase; regulates spore wall formation and septin organization, required for expression of some late meiotic genes and for normal localization of Glc7p
S000000250	YBR046C	ZTA1	ZeTA-crystallin	330507	331511	-1	NADPH-dependent quinone reductase	NADPH-dependent quinone reductase; GFP-tagged protein localizes to the cytoplasm and nucleus; has similarity to E. coli quinone oxidoreductase and to human zeta-crystallin
S000000251	YBR047W	FMP23	Found in Mitochondrial Proteome	331833	332360	1	Putative protein of unknown function	Putative protein of unknown function; proposed to be involved in iron or copper homeostasis; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
S000000252	YBR048W	RPS11B	Ribosomal Protein of the Small subunit	332831	333812	1	Protein component of the small (40S) ribosomal subunit	Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; RPS11B has a paralog, RPS11A, that arose from the whole genome duplication
S000000254	YBR050C	REG2	REsistance to Glucose repression	337183	338199	-1	Regulatory subunit of the Glc7p type-1 protein phosphatase	Regulatory subunit of the Glc7p type-1 protein phosphatase; involved with Reg1p, Glc7p, and Snf1p in regulation of glucose-repressible genes, also involved in glucose-induced proteolysis of maltose permease; REG2 has a paralog, REG1, that arose from the whole genome duplication
S000000255	YBR051W	""	""	337988	338338	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the REG2/YBR050C regulatory subunit of the Glc7p type-1 protein phosphatase
S000000256	YBR052C	RFS1	Rad55 (Fifty-five) Suppressor	338720	339352	-1	Protein of unknown function	Protein of unknown function; member of a flavodoxin-like fold protein family that includes Pst2p and Ycp4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; RFS1 has a paralog, PST2, that arose from the whole genome duplication
S000000257	YBR053C	""	""	339675	340751	-1	Putative protein of unknown function	Putative protein of unknown function; induced by cell wall perturbation
S000000258	YBR054W	YRO2	""	343101	344135	1	Protein with a putative role in response to acid stress	Protein with a putative role in response to acid stress; null mutant is sensitive to acetic acid; transcription is regulated by Haa1p and induced in the presence of acetic acid; protein observed in plasma membrane foci in the presence of acetic acid; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies
S000000259	YBR055C	PRP6	Pre-mRNA Processing	344602	347301	-1	Splicing factor	Splicing factor; component of the U4/U6-U5 snRNP complex
S000000260	YBR056W	MRX18	Mitochondrial oRganization of gene eXpression (MIOREX)	347879	349384	1	Putative glycoside hydrolase of the mitochondrial intermembrane space	Putative glycoside hydrolase of the mitochondrial intermembrane space
S000000261	YBR057C	MUM2	MUddled Meiosis	352193	353293	-1	Protein essential for meiotic DNA replication and sporulation	Protein essential for meiotic DNA replication and sporulation; cytoplasmic protein; subunit of the MIS complex which controls mRNA methylation during during the induction of sporulation; also interacts with Orc2p, which is a component of the origin recognition complex
S000000262	YBR058C	UBP14	UBiquitin-specific Protease	353672	356017	-1	Ubiquitin-specific protease	Ubiquitin-specific protease; specifically disassembles unanchored ubiquitin chains; involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; similar to human isopeptidase T
S000000263	YBR059C	AKL1	Ark family Kinase-Like protein	356861	360187	-1	Ser/Thr protein kinase	Ser/Thr protein kinase; phosphorylates Pan1p, Sla1p and Ent1p to negatively regulate endocytosis in response to membrane stress; regulates actin cytoskeleton organization and clathrin-dependent endocytosis; phosphorylated and inhibited by upstream kinase, Fpk1p; member, along with Ark1p and Prk1p, of the Ark kinase family 
S000000264	YBR060C	ORC2	Origin Recognition Complex	360652	362514	-1	Subunit of the origin recognition complex (ORC)	Subunit of the origin recognition complex (ORC); ORC directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; interacts with Spp1p and with trimethylated histone H3; phosphorylated by Cdc28p
S000000265	YBR061C	TRM7	Transfer RNA Methyltransferase	364787	365719	-1	2'-O-ribose methyltransferase	2'-O-ribose methyltransferase; methylates the 2'-O-ribose of tRNA-Phe, tRNA-Trp, and tRNA-Leu at positions C32 and N34 of tRNA anticodon loop; crucial biological role likely modification of tRNA-Phe; interacts with Trm732p and Rtt10p in 2'-O-methylation of C32 and N34 substrate tRNAs, respectively; yeast null mutant can be functionally complemented by human FTSJ1, mutations in which have been implicated in nonsyndromic X-linked intellectual disability (NSXLID)
S000000266	YBR062C	""	""	365976	366600	-1	Protein of unknown function that interacts with Msb2p	Protein of unknown function that interacts with Msb2p; may play a role in activation of the filamentous growth pathway
S000000267	YBR063C	CNM1	Contact Nucleus Mitochondria	366970	368184	-1	Molecular tether on nuclear membrane	Molecular tether on nuclear membrane; mediates contact by interacting with Tom70p on mitochondria; protein abundance is regulated by phosphatidylcholine, enabling coupling of phospholipid homeostasis with contact extent
S000000268	YBR064W	""	""	367763	368191	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR063C
S000000269	YBR065C	ECM2	ExtraCellular Mutant	368584	369678	-1	Pre-mRNA splicing factor	Pre-mRNA splicing factor; facilitates the cooperative formation of U2/U6 helix II in association with stem II in the spliceosome, function may be regulated by Slu7p
S000000270	YBR066C	NRG2	Negative Regulator of Glucose-controlled genes	370037	370699	-1	Transcriptional repressor	Transcriptional repressor; mediates glucose repression and negatively regulates filamentous growth; activated in stochastic pulses of nuclear localization in response to low glucose
S000000271	YBR067C	TIP1	Temperature shock-Inducible Protein	372103	372735	-1	Major cell wall mannoprotein with possible lipase activity	Major cell wall mannoprotein with possible lipase activity; transcription is induced by heat- and cold-shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins
S000000272	YBR068C	BAP2	Branched-chain Amino acid Permease	373861	375690	-1	High-affinity leucine permease	High-affinity leucine permease; functions as a branched-chain amino acid permease involved in uptake of leucine, isoleucine and valine; contains 12 predicted transmembrane domains; BAP2 has a paralog, BAP3, that arose from the whole genome duplication
S000000273	YBR069C	TAT1	Tyrosine and tryptophan Amino acid Transporter	376574	378433	-1	Amino acid transporter for valine, leucine, isoleucine, and tyrosine	Amino acid transporter for valine, leucine, isoleucine, and tyrosine; low-affinity tryptophan and histidine transporter; overexpression confers FK506 and FTY720 resistance; protein abundance increases in response to DNA replication stress
S000000274	YBR070C	ALG14	Asparagine Linked Glycosylation	379221	379934	-1	Component of UDP-GlcNAc transferase	Component of UDP-GlcNAc transferase; required for second step of dolichyl-linked oligosaccharide synthesis; anchors catalytic subunit Alg13p to ER membrane; localizes to the peroxisome; similar to bacterial and human glycosyltransferases; both human homologs ALG13 and ALG14 are required to complement yeast alg14 mutant; mutations of human ALG14 cause congenital myasthenic syndrome
S000000275	YBR071W	""	""	380411	381046	1	Protein of unknown function found in the cytoplasm and bud neck	Protein of unknown function found in the cytoplasm and bud neck; mRNA expression may be regulated by the cell cycle and/or cell wall stress; overexpression of YBR071W affects endocytic protein trafficking
S000000276	YBR072W	HSP26	Heat Shock Protein	382030	382674	1	Small heat shock protein (sHSP) with chaperone activity	Small heat shock protein (sHSP) with chaperone activity; forms hollow, sphere-shaped oligomers that suppress unfolded proteins aggregation; long-lived protein that is preferentially retained in mother cells and forms cytoplasmic foci; oligomer activation requires heat-induced conformational change; also has mRNA binding activity
S000000277	YBR073W	RDH54	RaD54 Homolog	383112	385988	1	DNA-dependent ATPase	DNA-dependent ATPase; DNA recombination/repair translocase, supercoils DNA, promotes DNA strand opening; stimulates strand exchange by modifying dsDNA topology; involved in recombinational repair of DNA double-strand breaks (DSBs) during mitosis, meiosis; implicated in cell divisions in telomerase-negative strains through adaptation to DNA damage, which allows bypass of cell cycle arrest and contributes senescence-specific genome instability; phosphorylated in response to DNA damage
S000000278	YBR074W	PFF1	Protease in FXNA-related Family	386286	389216	1	Multi-spanning vacuolar membrane protease	Multi-spanning vacuolar membrane protease; glycosylated transmembrane protein bearing homology to M28 family of metalloproteases; has lumenal-facing protease domain; proposed role in vacuole physiology; targeted to vacuole via AP-3 pathway
S000000279	YBR075W	""	""	387793	389175	1	Merged open reading frame	Merged open reading frame; does not encode a discrete protein; YBR075W was originally annotated as an independent ORF, but as a result of a sequence change, it was merged with an adjacent ORF into a single reading frame, designated YBR074W
S000000280	YBR076W	ECM8	ExtraCellular Mutant	390227	391291	1	Non-essential protein of unknown function	Non-essential protein of unknown function
S000000281	YBR077C	SLM4	Synthetic Lethal with Mss4	391805	392293	-1	Subunit of EGO/GSE complex	Subunit of EGO/GSE complex; vacuolar/endosomal membrane-associated EGO/GSE complex regulates exit from rapamycin-induced growth arrest, stimulating microautophagy and sorting of Gap1p from endosome to plasma membrane; essential for integrity and function of EGO; targeted to vacuole via AP-3 pathway; gene exhibits synthetic genetic interaction with MSS4
S000000282	YBR078W	ECM33	ExtraCellular Mutant	393123	394742	1	GPI-anchored protein involved in efficient glucose uptake	GPI-anchored protein involved in efficient glucose uptake; possible role in apical bud growth; GPI-anchoring on the plasma membrane crucial to function; phosphorylated in mitochondria; similar to Sps2p; ECM33 has a paralog, PST1, that arose from the whole genome duplication
S000000283	YBR079C	RPG1	""	395383	398277	-1	eIF3a subunit of the eukaryotic translation initiation factor 3 (eIF3)	eIF3a subunit of the eukaryotic translation initiation factor 3 (eIF3); subunit of the core complex of eIF3; part of a Prt1p-Rpg1p-Nip1p subcomplex that stimulates binding of mRNA and tRNA(i)Met to ribosomes; essential for loading mRNA onto ribosome at entry and exit channels of preinitiation complex; involved in translation reinitiation; eIF3 is also involved in programmed stop codon readthrough
S000000284	YBR080C	SEC18	SECretory	398614	400890	-1	AAA ATPase and SNARE disassembly chaperone	AAA ATPase and SNARE disassembly chaperone; required for vesicular transport between ER and Golgi, the 'priming' step in homotypic vacuole fusion, autophagy, and protein secretion; releases Sec17p from SNAP complexes; has similarity to mammalian N-ethylmaleimide-sensitive factor (NSF)
S000000285	YBR081C	SPT7	SuPpressor of Ty's	401253	405251	-1	Subunit of the SAGA transcriptional regulatory complex	Subunit of the SAGA transcriptional regulatory complex; involved in proper assembly of the complex; also present as a C-terminally truncated form in the SLIK/SALSA transcriptional regulatory complex
S000000286	YBR082C	UBC4	UBiquitin-Conjugating	406628	407169	-1	Ubiquitin-conjugating enzyme (E2)	Ubiquitin-conjugating enzyme (E2); key E2 partner with Ubc1p for the anaphase-promoting complex (APC); mediates degradation of abnormal or excess proteins, including calmodulin and histone H3; regulates levels of DNA Polymerase-{alpha} to promote efficient and accurate DNA replication; interacts with many SCF ubiquitin protein ligases; component of the cellular stress response; UBC4 has a paralog, UBC5, that arose from the whole genome duplication
S000000287	YBR083W	TEC1	Transposon Enhancement Control	409169	410629	1	Transcription factor targeting filamentation genes and Ty1 expression	Transcription factor targeting filamentation genes and Ty1 expression; Ste12p activation of most filamentation gene promoters depends on Tec1p and Tec1p transcriptional activity is dependent on its association with Ste12p; binds to TCS elements upstream of filamentation genes, which are regulated by Tec1p/Ste12p/Dig1p complex; competes with Dig2p for binding to Ste12p/Dig1p; positive regulator of chronological life span; TEA/ATTS DNA-binding domain family member
S000000288	YBR084W	MIS1	MItochondrial C1-tetrahydrofolate Synthase	411054	413981	1	Mitochondrial C1-tetrahydrofolate synthase	Mitochondrial C1-tetrahydrofolate synthase; involved in interconversion between different oxidation states of tetrahydrofolate (THF); provides activities of formyl-THF synthetase, methenyl-THF cyclohydrolase, and methylene-THF dehydrogenase
S000000289	YBR085W	AAC3	ADP/ATP Carrier	415983	416906	1	Mitochondrial inner membrane ADP/ATP translocator	Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; expressed under anaerobic conditions; similar to Aac1p; has roles in maintenance of viability and in respiration; AAC3 has a paralog, PET9, that arose from the whole genome duplication
S000000291	YBR087W	RFC5	Replication Factor C	423765	424829	1	Subunit of heteropentameric Replication factor C (RF-C)	Subunit of heteropentameric Replication factor C (RF-C); RF-C is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
S000000292	YBR088C	POL30	POLymerase	424990	425766	-1	Proliferating cell nuclear antigen (PCNA)	Proliferating cell nuclear antigen (PCNA); functions as the sliding replication clamp for DNA polymerase δ; may function as docking site for other proteins required for mitotic and meiotic chromosomal DNA replication and for DNA repair; PCNA ubiquitination at K164 plays crucial role during Okazaki fragment processing; Leu164 is essential for stable complex formation with replicative DNA polymerase δ
S000000293	YBR089W	""	""	425183	425782	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene POL30
S000000294	YBR090C	""	""	426333	427058	-1	Putative protein of unknown function	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
S000000295	YBR091C	TIM12	Translocase of the Inner Membrane	427155	427484	-1	Essential protein of the inner mitochondrial membrane	Essential protein of the inner mitochondrial membrane; peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins
S000000296	YBR092C	PHO3	PHOsphate metabolism	427698	429101	-1	Constitutively expressed acid phosphatase similar to Pho5p	Constitutively expressed acid phosphatase similar to Pho5p; brought to the cell surface by transport vesicles; hydrolyzes thiamin phosphates in the periplasmic space, increasing cellular thiamin uptake; expression is repressed by thiamin
S000000297	YBR093C	PHO5	PHOsphate metabolism	429548	430951	-1	Repressible acid phosphatase	Repressible acid phosphatase; 1 of 3 repressible acid phosphatases that also mediates extracellular nucleotide-derived phosphate hydrolysis; secretory pathway derived cell surface glycoprotein; induced by phosphate starvation and coordinately regulated by PHO4 and PHO2
S000000298	YBR094W	PBY1	P-BodY associated protein	432036	434297	1	Putative tubulin tyrosine ligase associated with P-bodies	Putative tubulin tyrosine ligase associated with P-bodies; may have a role in mRNA metabolism; yeast knockout collection strain identified as a pby1 null mutant is actually wild-type for PBY1 and deleted for mms4
S000000299	YBR095C	RXT2	""	434406	435698	-1	Component of the histone deacetylase Rpd3L complex	Component of the histone deacetylase Rpd3L complex; possibly involved in cell fusion and invasive growth; relocalizes to the cytosol in response to hypoxia
S000000301	YBR097W	VPS15	Vacuolar Protein Sorting	436951	441315	1	Serine/threonine protein kinase involved in vacuolar protein sorting	Serine/threonine protein kinase involved in vacuolar protein sorting; functions as a membrane-associated complex with Vps34p; active form recruits Vps34p to the Golgi membrane; interacts with the GDP-bound form of Gpa1p; myristoylated; a fraction is localized, with Vps34p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery
S000000302	YBR098W	MMS4	Methyl MethaneSulfonate sensitivity	441515	443590	1	Subunit of structure-specific Mms4p-Mus81p endonuclease	Subunit of structure-specific Mms4p-Mus81p endonuclease; cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdc5p during mitotic cell cycle activates the function of Mms4p-Mus81p
S000000303	YBR099C	""	""	442924	443307	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4
S000000304	YBR100W	""	""	443211	443549	1	Merged open reading frame	Merged open reading frame; does not encode a discrete protein; YBR100W was originally annotated as an independent ORF, but as a result of a sequence change, it was merged with an adjacent ORF into a single reading frame, designated YBR098W
S000000305	YBR101C	FES1	Factor Exchange for Ssa1p	443821	444693	-1	Hsp70 (Ssa1p) nucleotide exchange factor	Hsp70 (Ssa1p) nucleotide exchange factor; required for the release of misfolded proteins from the Hsp70 system to the Ub-proteasome machinery for degradation; cytosolic homolog of Sil1p, which is the nucleotide exchange factor for BiP (Kar2p) in the endoplasmic reticulum; protein abundance increases in response to DNA replication stress
S000000306	YBR102C	EXO84	EXOcyst	445062	447323	-1	Exocyst subunit with dual roles in exocytosis and spliceosome assembly	Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting of the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate
S000000307	YBR103W	SIF2	Sir4p-Interacting Factor	447709	449316	1	WD40 repeat-containing subunit of Set3C histone deacetylase complex	WD40 repeat-containing subunit of Set3C histone deacetylase complex; complex represses early/middle sporulation genes; antagonizes telomeric silencing; binds specifically to the Sir4p N-terminus
S000000308	YBR104W	YMC2	Yeast Mitochondrial Carrier	449667	450656	1	Putative mitochondrial inner membrane transporter	Putative mitochondrial inner membrane transporter; proposed role in oleate metabolism and glutamate biosynthesis; member of the mitochondrial carrier (MCF) family; YMC2 has a paralog, YMC1, that arose from the whole genome duplication
S000000309	YBR105C	VID24	Vacuolar Import and Degradation	450881	451969	-1	GID Complex regulatory subunit	GID Complex regulatory subunit; binds GID Complex in response to glucose through interactions with complex member Vid28p; regulates fructose-1,6-bisphosphatase (FBPase) targeting to the vacuole; promotes proteasome-dependent catabolite degradation of FBPase; peripheral membrane protein located at Vid (vacuole import and degradation) vesicles
S000000310	YBR106W	SND3	SRP-iNDependent targeting	452658	453224	1	Involved in SRP-independent protein targeting to the ER	Involved in SRP-independent protein targeting to the ER; component of an alternative ER-targeting pathway partially redundant with the GET pathway that can compensate for SRP loss; preference for substrates with downstream transmembrane domains; interacts with Snd1p, Env10p/Snd2p, and Sec61p-translocon subunits; role in establishing nucleus-vacuole junctions (NVJs) in high glucose; targeted to the NVJ during glucose starvation to facilitate expansion; role in phosphate transport with Pho86p
S000000311	YBR107C	IML3	Increased Minichromosome Loss	453793	454530	-1	Outer kinetochore protein and component of the Ctf19 complex	Outer kinetochore protein and component of the Ctf19 complex; involved in the establishment of pericentromeric cohesion during mitosis; prevents non-disjunction of sister chromatids during meiosis II; forms a stable complex with Chl4p; required for localization of Sgo1p to pericentric sites during meiosis I; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-L and fission yeast fta1
S000000312	YBR108W	AIM3	Altered Inheritance rate of Mitochondria	454822	457665	1	Protein that inhibits barbed-end actin filament elongation	Protein that inhibits barbed-end actin filament elongation; interacts with Rvs167p; null mutant is viable and displays elevated frequency of mitochondrial genome loss
S000000313	YBR109C	CMD1	CalMoDulin	457919	458362	-1	Calmodulin	Calmodulin; Ca2+ binding protein that regulates Ca2+ independent processes (mitosis, bud growth, actin organization, endocytosis, etc.) and Ca2+ dependent processes (stress-activated pathways), targets include Nuf1p, Myo2p and calcineurin; binds to the Hog1p MAPK in response to hyperosmotic stress; potentiates membrane tubulation and constriction mediated by the Rvs161p-Rvs167p complex; human CALM1 or CALM2 functionally complement repression induced inviability
S000000314	YBR110W	ALG1	Asparagine-Linked Glycosylation	458872	460221	1	Mannosyltransferase	Mannosyltransferase; involved in asparagine-linked glycosylation in the endoplasmic reticulum (ER); essential for viability; human homolog ALG1 complements yeast null mutant
S000000315	YBR111C	YSA1	""	461178	461873	-1	Nudix hydrolase family member with ADP-ribose pyrophosphatase activity	Nudix hydrolase family member with ADP-ribose pyrophosphatase activity; shown to metabolize O-acetyl-ADP-ribose to AMP and acetylated ribose 5'-phosphate
S000000316	YBR112C	CYC8	CYtochrome C	462870	465770	-1	General transcriptional co-repressor	General transcriptional co-repressor; acts together with Tup1p; also acts as part of a transcriptional co-activator complex that recruits the SWI/SNF and SAGA complexes to promoters; can form the prion [OCT+]
S000000317	YBR113W	""	""	465566	466048	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CYC8
S000000318	YBR114W	RAD16	RADiation sensitive	467248	469620	1	Nucleotide excision repair (NER) protein	Nucleotide excision repair (NER) protein; binds damaged DNA during NER; binds DNA in an ATP-dependent manner (with Rad7p) during NER; required for NER of non-transcribed chromatin; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex
S000000319	YBR115C	LYS2	LYSine requiring	469748	473926	-1	Alpha aminoadipate reductase	Alpha aminoadipate reductase; catalyzes the reduction of alpha-aminoadipate to alpha-aminoadipate 6-semialdehyde, which is the fifth step in biosynthesis of lysine; activation requires posttranslational phosphopantetheinylation by Lys5p
S000000320	YBR116C	""	""	474199	474726	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene TKL2
S000000323	YBR119W	MUD1	Mutant U1 Die	479338	480323	1	U1 snRNP A protein	U1 snRNP A protein; homolog of human U1-A; involved in nuclear mRNA splicing
S000000324	YBR120C	CBP6	Cytochrome B Protein synthesis	480435	480923	-1	Mitochondrial protein required for translation of the COB mRNA	Mitochondrial protein required for translation of the COB mRNA; forms a complex with Cbp3p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex; Cbp3p-Cbp6p complex is sequestered if assembly of Complex III is blocked, downregulating COB mRNA translation
S000000325	YBR121C	GRS1	Glycyl-tRNA Synthase	481364	483367	-1	Cytoplasmic and mitochondrial glycyl-tRNA synthase	Cytoplasmic and mitochondrial glycyl-tRNA synthase; ligates glycine to the cognate anticodon-bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation; GRS1 has a paralog, GRS2, that arose from the whole genome duplication; human homolog GARS implicated in Charcot-Marie-Tooth disease, can complement yeast null mutant
S000000326	YBR122C	MRPL36	Mitochondrial Ribosomal Protein, Large subunit	483970	484503	-1	Mitochondrial ribosomal protein of the large subunit	Mitochondrial ribosomal protein of the large subunit; overproduction suppresses mutations in the COX2 leader peptide-encoding region
S000000328	YBR124W	""	""	486507	486866	1	Putative protein of unknown function	Putative protein of unknown function
S000000329	YBR125C	PTC4	Phosphatase Two C	487199	488380	-1	Cytoplasmic type 2C protein phosphatase (PP2C)	Cytoplasmic type 2C protein phosphatase (PP2C); identified as a high-copy number suppressor of cnb1 mpk1 synthetic lethality; overexpression decreases high-osmolarity induced Hog1p phosphorylation and kinase activity
S000000330	YBR126C	TPS1	Trehalose-6-Phosphate Synthase	488905	490392	-1	Synthase subunit of trehalose-6-P synthase/phosphatase complex	Synthase subunit of trehalose-6-P synthase/phosphatase complex; synthesizes the storage carbohydrate trehalose, which is critically important for survival of long-term desiccation; also found in a monomeric form; expression is induced by the stress response and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress and in response to prolonged exposure to boric acid; required for pseudohyphal growth
S000000331	YBR127C	VMA2	Vacuolar Membrane Atpase	491269	492822	-1	Subunit B of V1 peripheral membrane domain of vacuolar H+-ATPase	Subunit B of V1 peripheral membrane domain of vacuolar H+-ATPase; electrogenic proton pump found throughout the endomembrane system; contains nucleotide binding sites; also detected in the cytoplasm; protein abundance increases in response to DNA replication stress; human homolog ATP6V1B1, implicated in autosomal-recessive distal renal tubular acidosis (RTA) with sensorineural deafness, complements yeast null mutant
S000000332	YBR128C	ATG14	AuTophaGy related	493081	494115	-1	Autophagy-specific subunit of phosphatidylinositol 3-kinase complex I	Autophagy-specific subunit of phosphatidylinositol 3-kinase complex I; Atg14p targets complex I to the phagophore assembly site (PAS); required for localizing additional ATG proteins to the PAS; required for overflow degradation of misfolded proteins when ERAD is saturated; homolog of human Barkor; other members are Vps34, Vps15, and Vps30p
S000000333	YBR129C	OPY1	Overproduction-induced Pheromone-resistant Yeast	494353	495339	-1	Protein of unknown function	Protein of unknown function; overproduction blocks cell cycle arrest in the presence of mating pheromone; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
S000000334	YBR130C	SHE3	Swi5p-dependent HO Expression	495592	496869	-1	Protein adaptor between Myo4p and the She2p-mRNA complex	Protein adaptor between Myo4p and the She2p-mRNA complex; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud; also required for cortical ER inheritance
S000000335	YBR131W	CCZ1	Calcium Caffeine Zinc sensitivity	497163	499277	1	Subunit of a heterodimeric guanine nucleotide exchange factor (GEF)	Subunit of a heterodimeric guanine nucleotide exchange factor (GEF); subunit of the Mon1-Ccz1 GEF complex, which stimulates nucleotide exchange and activation of Ypt7p, a Rab family GTPase involved in membrane tethering and fusion events at the late endosome and vacuole; GEF activity is stimulated by membrane association and anionic phospholipids; involved in localizing Ypt7p to the vacuolar membrane; required for macroautophagy, the CVT pathway and mitophagy
S000000336	YBR132C	AGP2	high-Affinity Glutamine Permease	499652	501442	-1	Plasma membrane regulator of high affinity polyamine and carnitine tra	Plasma membrane regulator of high affinity polyamine and carnitine transport; has similarity to transporters but lacks transport activity; may act as a sensor that transduces environmental signals; has a positive or negative regulatory effect on transcription of many transporter genes; null mutant is cycloheximide (CHX) resistant; ubiquitinated Agp2p is rapidly degraded in a Brp1p-dependent manner in the presence of CHX; localizes to the plasma membrane, the vacuolar membrane and the ER membrane
S000000337	YBR133C	HSL7	Histone Synthetic Lethal	501804	504287	-1	Protein arginine N-methyltransferase	Protein arginine N-methyltransferase; exhibits septin and Hsl1p-dependent localization to the bud neck in budded cells and periodic Hsl1p-dependent phosphorylation; required with Hsl1p, and Elm1p for the mother-bud neck recruitment, phosphorylation, and degradation of Swe1p; responsible for symmetric dimethylation of histone H4 on Arg3; relocalizes away from bud neck upon DNA replication stress; human homolog PRMT5 can complement yeast hsl7 mutant
S000000338	YBR134W	""	""	504243	504644	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000000339	YBR135W	CKS1	Cdc28 Kinase Subunit	504854	505306	1	Cyclin-dependent protein kinase regulatory subunit and adaptor	Cyclin-dependent protein kinase regulatory subunit and adaptor; interacts with Cdc28p (aka Cdk1p); required for G1/S and G2/M phase transitions and budding; mediates phosphorylation and degradation of Sic1p; modulates proteolysis of M-phase targets through interactions with the proteasome; role in transcriptional regulation, recruiting proteasomal subunits to target gene promoters; human homologs CKS1B and CKS2 can each complement yeast cks1 null mutant
S000000341	YBR137W	""	""	513044	513583	1	Protein with a role in ER delivery of tail-anchored membrane proteins	Protein with a role in ER delivery of tail-anchored membrane proteins; interacts with Sgt2p; binds to the TRC complex, which inserts proteins into the ER membrane; interacts with Msn5p karyopherin; YBR137W is not an essential gene
S000000342	YBR138C	""	""	513762	515336	-1	Cytoplasmic protein of unknown function	Cytoplasmic protein of unknown function; APCC(Cdh1) substrate; potentially phosphorylated by Cdc28p; YBR138C is not an essential gene
S000000343	YBR139W	ATG42	AuTophaGy	515664	517190	1	Vacuolar serine-type carboxypeptidase	Vacuolar serine-type carboxypeptidase; involved, along with functional homolog Prc1p, in vacuolar zymogen activation, breakdown of the autophagic body, and autophagosome-dependent protein synthesis; role in phytochelatin synthesis; localizes to the vacuole lumen; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner; N-glycosylated
S000000344	YBR140C	IRA1	Inhibitory Regulator of the RAS-cAMP pathway	517350	526628	-1	GTPase-activating protein	GTPase-activating protein; negatively regulates RAS by converting it from GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions, mediates membrane association of adenylate cyclase; mutations cause catalase T deficiency, defective glycogen synthesis and defective trehalose accumulation; IRA1 has a paralog, IRA2, that arose from the whole genome duplication; defects in human homolog NF1 are associated with neurofibromatosis
S000000345	YBR141C	BMT2	Base MethylTransferase of 25S RNA	527025	528038	-1	Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase	Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of large subunit (LSU) rRNA at position 2142; belongs to Rossmann fold superfamily; null mutant exhibits abnormal lipid metabolic phenotype; not an essential gene
S000000346	YBR142W	MAK5	MAintenance of Killer	528317	530638	1	Essential nucleolar protein	Essential nucleolar protein; putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits
S000000347	YBR143C	SUP45	SUPpressor	530869	532182	-1	Polypeptide release factor (eRF1) in translation termination	Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p
S000000348	YBR144C	""	""	533235	533549	-1	Putative protein of unknown function	Putative protein of unknown function; conserved across S. cerevisiae strains; YBR144C is not an essential gene
S000000349	YBR145W	ADH5	Alcohol DeHydrogenase	533762	534817	1	Alcohol dehydrogenase isoenzyme V	Alcohol dehydrogenase isoenzyme V; involved in ethanol production; ADH5 has a paralog, ADH1, that arose from the whole genome duplication
S000000350	YBR146W	MRPS9	Mitochondrial Ribosomal Protein, Small subunit	535260	536096	1	Mitochondrial ribosomal protein of the small subunit	Mitochondrial ribosomal protein of the small subunit
S000000351	YBR147W	RTC2	Restriction of Telomere Capping	536575	537465	1	Putative vacuolar membrane transporter for cationic amino acids	Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; positive regulation by Lys14p suggests that lysine may be the primary substrate; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter; RTC2 has a paralog, YPQ1, that arose from the whole genome duplication
S000000352	YBR148W	YSW1	""	537876	539705	1	Protein required for normal prospore membrane formation	Protein required for normal prospore membrane formation; interacts with Gip1p, which is the meiosis-specific regulatory subunit of the Glc7p protein phosphatase; expressed specifically in spores and localizes to the prospore membrane; YSW1 has a paralog, SPO21, that arose from the whole genome duplication
S000000353	YBR149W	ARA1	D-ARAbinose dehydrogenase	539987	541021	1	NADP+ dependent arabinose dehydrogenase	NADP+ dependent arabinose dehydrogenase; involved in carbohydrate metabolism; purified as homodimer; naturally occurs with a N-terminus degradation product
S000000354	YBR150C	TBS1	ThiaBendazole Sensitive	541209	544493	-1	Protein of unknown function	Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; associates with rDNA chromatin in vivo; TBS1 has a paralog, HAL9, that arose from the whole genome duplication
S000000355	YBR151W	APD1	Actin Patches Distal	545028	545978	1	2Fe-2S cytosolic protein of unknown function	2Fe-2S cytosolic protein of unknown function; bishistidinyl coordinated, non-Rieske [2Fe-2S] cluster containing thiredoxin-like ferredoxin; required for normal localization of actin patches and for normal tolerance of sodium ions and hydrogen peroxide; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm; homologous to AIM32
S000000356	YBR152W	SPP381	Suppressor of PrP38-1	546376	547251	1	mRNA splicing factor, component of U4/U6.U5 tri-snRNP	mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p; relocalizes to the cytosol in response to hypoxia; temperature-sensitive phenotype of prp38-1 mutant can be suppressed by human homolog of SPP381, MFAP1
S000000357	YBR153W	RIB7	RIBoflavin biosynthesis	547460	548194	1	Diaminohydroxyphoshoribosylaminopyrimidine deaminase	Diaminohydroxyphoshoribosylaminopyrimidine deaminase; catalyzes the second step of the riboflavin biosynthesis pathway
S000000358	YBR154C	RPB5	RNA Polymerase B	548362	549009	-1	RNA polymerase subunit ABC27	RNA polymerase subunit ABC27; common to RNA polymerases I, II, and III; contacts DNA and affects transactivation
S000000359	YBR155W	CNS1	CyclophiliN Seven suppressor	549771	550928	1	TPR-containing co-chaperone	TPR-containing co-chaperone; binds both Hsp82p (Hsp90) and Ssa1p (Hsp70); stimulates ATPase activity of Ssa1p; ts mutants reduce Hsp82p function, overexpression suppresses phenotypes of HSP82 ts allele and cpr7 deletion; human homolog TTC4 complements yeast cns1 mutant
S000000360	YBR156C	SLI15	Synthetically Lethal with Ipl1	551104	553200	-1	Subunit of the conserved chromosomal passenger complex (CPC)	Subunit of the conserved chromosomal passenger complex (CPC); complex regulates kinetochore-microtubule attachments, activation of the spindle tension checkpoint, and mitotic spindle disassembly; other complex members are Ipl1p, Bir1p, and Nbl1p
S000000361	YBR157C	ICS2	Increased Copper Sensitivity	553543	554310	-1	Protein of unknown function	Protein of unknown function; null mutation does not confer any obvious defects in growth, spore germination, viability, or carbohydrate utilization
S000000362	YBR158W	AMN1	Antagonist of Mitotic exit Network	556549	558198	1	Modulator of cell separation and mitotic exit	Modulator of cell separation and mitotic exit; inhibits separation through Ub-dependent Ace2p proteolysis; part of a daughter-specific switch induced by the mitotic exit network that inhibits exit and resets the cell cycle after the execution of  MEN function, blocking Tem1p and Cdc15 association; required for chromosome stability and multiple mitotic checkpoints; regulated by SCF; haploid transcription regulated by Ste12p; contains 12 degenerate leucine-rich repeat motifs and an atypical F-box
S000000363	YBR159W	IFA38	""	558685	559728	1	Microsomal beta-keto-reductase	Microsomal beta-keto-reductase; contains oleate response element (ORE) sequence in the promoter region; mutants exhibit reduced VLCFA synthesis, accumulate high levels of dihydrosphingosine, phytosphingosine and medium-chain ceramides
S000000364	YBR160W	CDC28	Cell Division Cycle	560078	560974	1	Cyclin-dependent kinase (CDK) catalytic subunit	Cyclin-dependent kinase (CDK) catalytic subunit; master regulator of mitotic and meiotic cell cycles; alternately associates with G1, S, G2/M phase cyclins, which provide substrate specificity; regulates metabolism, basal transcription, chromosome dynamics, growth and morphogenesis; transcript induction in osmostress involves antisense RNA; human homologs CDK1, CDK2, CDK3 can complement yeast conditional cdc28 mutants; human CDK1, CDK2 can complement yeast cdc28 null mutant
S000000365	YBR161W	CSH1	CSG1/SUR1 Homolog	561634	562764	1	Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit	Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p; CSH1 has a paralog, SUR1, that arose from the whole genome duplication
S000000366	YBR162C	TOS1	Target Of Sbf	563203	564570	-1	Covalently-bound cell wall protein of unknown function	Covalently-bound cell wall protein of unknown function; identified as a cell cycle regulated SBF target gene; deletion mutants are highly resistant to treatment with beta-1,3-glucanase; has sequence similarity to YJL171C
S000000367	YBR163W	EXO5	EXOnuclease V	565723	567480	1	Mitochondrial 5'-3' exonuclease and sliding exonuclease	Mitochondrial 5'-3' exonuclease and sliding exonuclease; required for mitochondrial genome maintenance; distantly related to the RecB nuclease domain of bacterial RecBCD recombinases; may be regulated by the transcription factor Ace2
S000000368	YBR164C	ARL1	ADP-Ribosylation factor-Like	567875	568426	-1	Soluble GTPase with a role in regulation of membrane traffic	Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; role in membrane organization at trans-Golgi network; required for delivery of Atg9p to the phagophore assembly site during autophagy under high temperature stress; required with Ypt6p for starvation-induced autophagy; required for the CVT pathway under non-starvation conditions; G protein of the Ras superfamily, similar to ADP-ribosylation factor
S000000369	YBR165W	UBS1	UBiquitin-conjugating enzyme Suppressor	568852	569685	1	Ubiquitin-conjugating enzyme suppressor that regulates Cdc34p	Ubiquitin-conjugating enzyme suppressor that regulates Cdc34p; functions as a general positive regulator of Cdc34p activity; nuclear protein that may represent a link between nucleocytoplasmic transport and ubiquitin ligase activity
S000000370	YBR166C	TYR1	TYRosine	569842	571200	-1	Prephenate dehydrogenase involved in tyrosine biosynthesis	Prephenate dehydrogenase involved in tyrosine biosynthesis; expression is dependent on phenylalanine levels
S000000371	YBR167C	POP7	Processing Of Precursor RNAs	571468	571890	-1	Subunit of RNase MRP, nuclear RNase P and telomerase	Subunit of RNase MRP, nuclear RNase P and telomerase; forms a soluble heterodimer with Pop6p that binds P3 domain of RNase MRP and RNase P RNAs; RNase MRP cleaves pre-rRNA, nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs, while telomerase replenishes telomeric DNA
S000000372	YBR168W	PEX32	PEroXisome related	572371	573612	1	Peroxisomal integral membrane protein	Peroxisomal integral membrane protein; involved in negative regulation of peroxisome size; partially functionally redundant with Pex31p; genetic interactions suggest action at a step downstream of steps mediated by Pex28p and Pex29p
S000000373	YBR169C	SSE2	Stress Seventy subfamily E	573915	575996	-1	Member of Hsp110 subclass of the heat shock protein 70 (HSP70) family	Member of Hsp110 subclass of the heat shock protein 70 (HSP70) family; serves as nucleotide exchange factor to load ATP onto the SSA class of cytosolic Hsp70s; may be involved in protein folding; localized to the cytoplasm; SSE2 has a paralog, SSE1, that arose from the whole genome duplication
S000000374	YBR170C	NPL4	Nuclear Protein Localization	576344	578086	-1	Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase	Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; K48-specific Ub chain binding receptor that assists Cdc48p in the dislocation of misfolded, polyubiquitinated ERAD substrates destined for proteasomal degradation; role in regulated destruction of ER membrane proteins, like HMG-CoA reductase (Hmg1/2p), cytosolic proteins (Fbp1p) and mislocalized proteins (Cse4p); mobilizes membrane-bound transcription factors by regulated Ub/proteasome-dependent processing; contains a UBX domain
S000000375	YBR171W	SEC66	SECretory	578364	578984	1	Non-essential subunit of Sec63 complex	Non-essential subunit of Sec63 complex; with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and translocation into the ER; other members are Sec63p, Sec62p, and Sec72p
S000000376	YBR172C	SMY2	Suppressor of MYo2-66	579150	581372	-1	GYF domain protein	GYF domain protein; involved in COPII vesicle formation; regulates Cdc48p function in transcription stress response; interacts with the Sec23p/Sec24p subcomplex; overexpression suppresses the temperature sensitivity of a myo2 mutant; homologous to human GIGYF1 and GIGYF2; similar to S. pombe Mpd2; SMY2 has a paralog, SYH1, that arose from the whole genome duplication
S000000377	YBR173C	UMP1	Ubiquitin-Mediated Proteolysis	581726	582172	-1	Chaperone required for correct maturation of the 20S proteasome	Chaperone required for correct maturation of the 20S proteasome; short-lived chaperone; may inhibit premature dimerization of proteasome half-mers; degraded by proteasome upon completion of its assembly
S000000378	YBR174C	""	""	582338	582652	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YBR175W; null mutant is viable and sporulation defective
S000000379	YBR175W	SWD3	Set1c, WD40 repeat protein	582408	583355	1	Essential subunit of the COMPASS (Set1C) complex	Essential subunit of the COMPASS (Set1C) complex; COMPASS methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres; WD40 beta propeller superfamily member and ortholog of mammalian WDR5
S000000380	YBR176W	ECM31	ExtraCellular Mutant	583720	584658	1	Ketopantoate hydroxymethyltransferase	Ketopantoate hydroxymethyltransferase; required for pantothenic acid biosynthesis, converts 2-oxoisovalerate into 2-dehydropantoate
S000000381	YBR177C	EHT1	Ethanol Hexanoyl Transferase	584807	586162	-1	Octanoyl-CoA:ethanol acyltransferase	Octanoyl-CoA:ethanol acyltransferase; also functions as thioesterase; plays a minor role in medium-chain fatty acid ethyl ester biosynthesis; localizes to lipid particles and the mitochondrial outer membrane; EHT1 has a paralog, EEB1, that arose from the whole genome duplication
S000000382	YBR178W	""	""	586071	586445	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR177C
S000000383	YBR179C	FZO1	FuZzy Onions homolog	586547	589114	-1	Mitofusin, protein involved in mitochondrial outer membrane fusion	Mitofusin, protein involved in mitochondrial outer membrane fusion; role in mitochondrial genome maintenance; efficient tethering and degradation of Fzo1p requires an intact N-terminal GTPase domain; targeted for destruction by the ubiquitin ligase SCF-Mdm30p and the cytosolic ubiquitin-proteasome system; activity regulated by ubiquitylation at conserved lysine residues and by  deubiquitylases Ubp2p and Ubp12p
S000000384	YBR180W	DTR1	DiTyRosine	589741	591459	1	Putative dityrosine transporter of the major facilitator superfamily	Putative dityrosine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; required for spore wall synthesis; sequence similarity to QDR1 and QDR3, and the triple mutant dtr1 qdr1 qdr3 exhibits reduced dityrosine fluorescence relative to the single mutants; expressed during sporulation
S000000385	YBR181C	RPS6B	Ribosomal Protein of the Small subunit	591712	592774	-1	Protein component of the small (40S) ribosomal subunit	Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; phosphorylated on S233 by Ypk3p in a TORC1-dependent manner, and on S232 in a TORC1/2-dependent manner by Ypk1/2/3p; RPS6B has a paralog, RPS6A, that arose from the whole genome duplication
S000000386	YBR182C	SMP1	Second MEF2-like Protein	593506	594864	-1	MADS-box transcription factor involved in osmotic stress response	MADS-box transcription factor involved in osmotic stress response; SMP1 has a paralog, RLM1, that arose from the whole genome duplication
S000000387	YBR183W	YPC1	Yeast Phyto-Ceramidase	596115	597065	1	Alkaline ceramidase	Alkaline ceramidase; also has reverse (CoA-independent) ceramide synthase activity; catalyzes both breakdown and synthesis of phytoceramide; overexpression confers fumonisin B1 resistance; YPC1 has a paralog, YDC1, that arose from the whole genome duplication
S000000388	YBR184W	""	""	597363	598934	1	Putative protein of unknown function	Putative protein of unknown function; YBR184W is not an essential gene
S000000389	YBR185C	MBA1	Multi-copy Bypass of AFG3	599123	599959	-1	Membrane-associated mitochondrial ribosome receptor	Membrane-associated mitoribosome receptor; involved along with Dpi29p in the cotranslational insertion of nascent polypeptide chains into the inner mitochondrial membrane; associates with nascent polypeptide chains; interacts with Mdm38p and may recruit mRNA-specific translational activators to the mitoribosome; null mutant displays decreased tolerance to mistranslation-induced proteotoxic stress; localizes to the inner mitochondrial membrane
S000000390	YBR186W	PCH2	Pachytene CHeckpoint	600553	602360	1	Hexameric ring ATPase that remodels chromosome axis protein Hop1p	Hexameric ring ATPase that remodels chromosome axis protein Hop1p; nucleolar component of the pachytene checkpoint, which prevents chromosome segregation when recombination and chromosome synapsis are defective; also represses meiotic interhomolog recombination in rDNA; required for meiotic double-stranded break formation
S000000391	YBR187W	GDT1	Gcr1 Dependent Translation factor	602634	603476	1	Calcium and manganese transporter with higher affinity for Ca2+	Calcium and manganese transporter with higher affinity for Ca2+; involved in Ca2+ and Mn2+ homeostasis; localizes to the cis- and medial-Golgi apparatus; GFP-fusion localizes to the vacuole; required for the Mn2+-dependent function of glycosylation enzymes; TMEM165, a human ortholog linked to Congenital Disorders of Glycosylation, functionally complements the null allele; expression pattern and physical interactions suggest a possible role in ribosome biogenesis; expression regulated by Gcr1p 
S000000392	YBR188C	NTC20	Prp19p (NineTeen)-associated Complex	603685	604107	-1	Member of the NineTeen Complex (NTC)	Member of the NineTeen Complex (NTC); this complex contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs
S000000393	YBR189W	RPS9B	Ribosomal Protein of the Small subunit	604508	605508	1	Protein component of the small (40S) ribosomal subunit	Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9B has a paralog, RPS9A, that arose from the whole genome duplication
S000000394	YBR190W	""	""	605966	606277	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ribosomal protein gene RPL21A/YBR191W
S000000395	YBR191W	RPL21A	Ribosomal Protein of the Large subunit	606270	607140	1	Ribosomal 60S subunit protein L21A	Ribosomal 60S subunit protein L21A; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21A has a paralog, RPL21B, that arose from the whole genome duplication
S000000396	YBR192W	RIM2	Replication In Mitochondria	607652	608785	1	Mitochondrial pyrimidine nucleotide transporter	Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family
S000000397	YBR193C	MED8	MEDiator complex	609082	609753	-1	Subunit of the RNA polymerase II mediator complex	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
S000000398	YBR194W	AIM4	Altered Inheritance rate of Mitochondria	610038	610409	1	Protein proposed to be associated with the nuclear pore complex	Protein proposed to be associated with the nuclear pore complex; null mutant is viable, displays elevated frequency of mitochondrial genome loss and is sensitive to freeze-thaw stress
S000000399	YBR195C	MSI1	Multicopy Suppressor of IRA1	610614	611882	-1	Subunit of chromatin assembly factor I (CAF-1)	Subunit of chromatin assembly factor I (CAF-1); chromatin assembly by CAF-1 affects multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of DNA damage checkpoint after DNA repair; chromatin dynamics during transcription; and repression of divergent noncoding transcription; Msi1p localizes to nucleus and cytoplasm and independently regulates the RAS/cAMP pathway via sequestration of Npr1p kinase
S000000400	YBR196C	PGI1	PhosphoGlucoIsomerase	612236	613900	-1	Glycolytic enzyme phosphoglucose isomerase	Glycolytic enzyme phosphoglucose isomerase; catalyzes the interconversion of glucose-6-phosphate and fructose-6-phosphate; required for cell cycle progression and completion of the gluconeogenic events of sporulation
S000000401	YBR197C	""	""	615203	615856	-1	Protein of unknown function	Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YBR197C is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress; YBR197C has a paralog, YPL077C, that arose from the whole genome duplication
S000000403	YBR199W	KTR4	Kre Two Related	618909	620303	1	Glycosyltransferase involved in protein glycosylation	Glycosyltransferase involved in protein glycosylation; transfers GDP-mannose to methyl-alpha-mannoside in vitro; member of the KRE2/MNT1 mannosyltransferase family of type II membrane proteins with a short cytoplasmic N-terminus, a membrane-spanning region and a highly conserved catalytic lumenal domain
S000000404	YBR200W	BEM1	Bud EMergence	620872	622527	1	Protein containing SH3-domains	Protein containing SH3-domains; involved in establishing cell polarity and morphogenesis; functions as a scaffold protein for complexes that include Cdc24p, Ste5p, Ste20p, and Rsr1p
S000000405	YBR201W	DER1	Degradation in the Endoplasmic Reticulum	623576	624211	1	ER membrane protein that promotes export of misfolded polypeptides	ER membrane protein that promotes export of misfolded polypeptides; required for ER-associated protein degradation of misfolded or unassembled proteins; initiates export of aberrant polypeptides from ER lumen by threading them into ER membrane and routing them to Hrd1p for ubiquitination; function normally requires N-terminal acetylation by NatB; N- and C- termini protrude into cytoplasm; similar to Dfm1p; homolog of mammalian derlin-1
S000000406	YBR202W	MCM7	MiniChromosome Maintenance	625772	628309	1	Component of the Mcm2-7 hexameric helicase complex	Component of the Mcm2-7 hexameric helicase complex; MCM2-7 primes origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation in S-phase; forms an Mcm4p-6p-7p subcomplex
S000000407	YBR203W	COS111	Ciclopirox Olamine Sensitive	629168	631942	1	Protein required for antifungal drug ciclopirox olamine resistance	Protein required for antifungal drug ciclopirox olamine resistance; not related to the subtelomerically-encoded COS family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
S000000408	YBR204C	LDH1	Lipid Droplet Hydrolase	632254	633381	-1	Serine hydrolase	Serine hydrolase; exhibits active esterase plus weak triacylglycerol lipase activities; proposed role in lipid homeostasis, regulating phospholipid and non-polar lipid levels and required for mobilization of LD-stored lipids; localizes to the lipid droplet (LD) surface; contains a classical serine containing catalytic triad (GxSxG motif)
S000000409	YBR205W	KTR3	Kre Two Related	633622	634836	1	Putative alpha-1,2-mannosyltransferase	Putative alpha-1,2-mannosyltransferase; involved in O- and N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; Svp26p mediates uptake of Ktr3p into COPII vesicles; relocalizes from nucleus to vacuole upon DNA replication stress
S000000410	YBR206W	""	""	634601	634924	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KTR3
S000000411	YBR207W	FTH1	FTR1 Homolog	635146	636543	1	Putative high affinity iron transporter	Putative high affinity iron transporter; involved in transport of intravacuolar stores of iron; forms complex with Fet5p; expression is regulated by iron; proposed to play indirect role in endocytosis; protein abundance increases in response to DNA replication stress
S000000412	YBR208C	DUR12	Degradation of URea	636703	642210	-1	Urea amidolyase	Urea amidolyase; contains both urea carboxylase and allophanate hydrolase activities, degrades urea to CO2 and NH3; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation; protein abundance increases in response to DNA replication stress
S000000413	YBR209W	""	""	642583	642900	1	Putative protein of unknown function	Putative protein of unknown function; conserved across S. cerevisiae strains; YBR209W is not an essential gene
S000000414	YBR210W	ERV15	ER Vesicle Protein	645550	645978	1	Protein involved in export of proteins from the endoplasmic reticulum	Protein involved in export of proteins from the endoplasmic reticulum; ERV15 has a paralog, ERV14, that arose from the whole genome duplication
S000000416	YBR212W	NGR1	Negative Growth Regulatory protein	647886	649904	1	RNA binding protein that negatively regulates growth rate	RNA binding protein that negatively regulates growth rate; interacts with the 3' UTR of the mitochondrial porin (POR1) mRNA and enhances its degradation; overexpression impairs mitochondrial function; interacts with Dhh1p to mediate POR1 mRNA decay; expressed in stationary phase
S000000417	YBR213W	MET8	METhionine requiring	650368	651192	1	Bifunctional dehydrogenase and ferrochelatase	Bifunctional dehydrogenase and ferrochelatase; involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis
S000000418	YBR214W	SDS24	homolog of S. pombe SDS23	651415	652998	1	Protein involved in cell separation during budding	Protein involved in cell separation during budding; one of two S. cerevisiae homologs (Sds23p and Sds24p) of the S. pombe Sds23 protein, which is implicated in APC/cyclosome regulation; may play an indirect role in fluid-phase endocytosis; protein abundance increases in response to DNA replication stress; SDS24 has a paralog, SDS23, that arose from the whole genome duplication
S000000419	YBR215W	HPC2	Histone Periodic Control	653356	655317	1	Subunit of the HIR complex	Subunit of the HIR complex; HIR is a nucleosome assembly complex involved in regulation of histone gene transcription; mutants display synthetic defects with subunits of FACT, a complex that allows passage of RNA Pol II through nucleosomes
S000000420	YBR216C	YBP1	Yap1-Binding Protein	655576	657600	-1	Protein involved in cellular response to oxidative stress	Protein involved in cellular response to oxidative stress; required for oxidation of specific cysteine residues of transcription factor Yap1p, resulting in nuclear localization of Yap1p in response to stress; YBP1 has a paralog, YBP2, that arose from the whole genome duplication
S000000421	YBR217W	ATG12	AuTophaGy related	657832	658392	1	Ubiquitin-like modifier involved in autophagy and the Cvt pathway	Ubiquitin-like modifier involved in autophagy and the Cvt pathway; conserved; conjugated to Atg5p to form a complex involved in Atg8p lipidation; Atg5p-Atg12p conjugate enhances E2 activity of Atg3p by rearranging its catalytic site, also forms a complex with Atg16p; the Atg5-Atg12/Atg16 complex binds to membranes and is essential for autophagosome formation; homolog of mammalian ULK1
S000000422	YBR218C	PYC2	PYruvate Carboxylase	658707	662249	-1	Pyruvate carboxylase isoform	Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc1p; mutations in the human homolog are associated with lactic acidosis; PYC2 has a paralog, PYC1, that arose from the whole genome duplication
S000000423	YBR219C	""	""	662499	663303	-1	Putative protein of unknown function	Putative protein of unknown function; YBR219C is not an essential gene
S000000424	YBR220C	""	""	662995	664677	-1	Putative protein of unknown function	Putative protein of unknown function; YBR220C is not an essential gene
S000000425	YBR221C	PDB1	Pyruvate Dehydrogenase Beta subunit	665153	666253	-1	E1 beta subunit of the pyruvate dehydrogenase (PDH) complex	E1 beta subunit of the pyruvate dehydrogenase (PDH) complex; PDH is an evolutionarily conserved multi-protein complex found in mitochondria
S000000426	YBR222C	PCS60	Peroxisomal CoA-dependent Synthetase	666720	668351	-1	Oxalyl-CoA synthetase	Oxalyl-CoA synthetase; capable of catalyzing conversion of oxalate to oxalyl-CoA; catalyzes first step in pathway of oxalate degradation that functions to protect yeast from inhibitory effects of oxalate; peroxisomal protein that binds mRNA; localizes to both peroxisomal peripheral membrane and matrix, expression is highly inducible by oleic acid; similar to E. coli long chain acyl-CoA synthetase
S000000427	YBR223C	TDP1	Tyrosyl-DNA Phosphodiesterase	668663	670297	-1	Tyrosyl-DNA phosphodiesterase I	Tyrosyl-DNA phosphodiesterase I; hydrolyzes 3' and 5'-phosphotyrosyl bonds; involved in the repair of DNA lesions created by topo I and topo II, including DNA-protein cross-links; mutations in the human homolog, TDP1, result in the neurodegenerative disease, spinocerebellar ataxia with axonal neuropathy (SCAN1); yeast cells and human rhabdomyosarcoma lines that overexpress TDP1 both exhibit elevated dosage chromosomal instability (dCIN)
S000000428	YBR224W	""	""	670125	670640	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene TDP1
S000000429	YBR225W	""	""	670627	673329	1	Putative protein of unknown function	Putative protein of unknown function; non-essential gene identified in a screen for mutants affected in mannosylphophorylation of cell wall components
S000000430	YBR226C	""	""	673155	673565	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YBR225W
S000000431	YBR227C	MCX1	Mitochondrial ClpX	673572	675134	-1	Non-proteolytic ATPase of the AAA family	Non-proteolytic ATPase of the AAA family; stimulates incorporation of the pyridoxal phosphate cofactor into Hem1p (5-aminolevulinic acid synthase); localized to the mitochondrial matrix; ortholog of vertebrate CLPX, which promotes erythropoiesis
S000000432	YBR228W	SLX1	Synthetic Lethal of unknown (X) function	675313	676227	1	Endonuclease involved in DNA recombination and repair	Endonuclease involved in DNA recombination and repair; subunit of a complex, with Slx4p, that hydrolyzes 5' branches from duplex DNA in response to stalled or converging replication forks; function overlaps with that of Sgs1p-Top3p
S000000433	YBR229C	ROT2	Reversal Of Tor2 lethality	676357	679221	-1	Glucosidase II catalytic subunit	Glucosidase II catalytic subunit; required to trim the final glucose in N-linked glycans; required for normal cell wall synthesis; mutations in rot2 suppress tor2 mutations, and are synthetically lethal with rot1 mutations
S000000434	YBR230C	OM14	Outer Membrane Protein of 14 kDa	679549	680050	-1	Mitochondrial outer membrane receptor for cytosolic ribosomes	Mitochondrial outer membrane receptor for cytosolic ribosomes; integral protein of the outer membrane that interacts with the nascent chain-associated complex (NAC) bound to ribosomes, contributing to co-translational mitochondrial import; interacts with porin (Por1p) and Om45p; abundance is decreased in cells grown in glucose relative to other carbon sources
S000000435	YBR231C	SWC5	SWr Complex	682179	683090	-1	Component of the SWR1 complex	Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress; relocalizes to the cytosol in response to hypoxia
S000000436	YBR232C	""	""	683373	683732	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000000437	YBR233W	PBP2	Pbp1p Binding Protein	683428	684669	1	RNA binding protein	RNA binding protein; has similarity to mammalian heterogeneous nuclear RNP K protein, involved in the regulation of telomere position effect and telomere length; relative distribution to the nucleus increases upon DNA replication stress
S000000438	YBR234C	ARC40	ARp2/3 Complex subunit	685438	686592	-1	Subunit of the ARP2/3 complex	Subunit of the ARP2/3 complex; ARP2/3 is required for the motility and integrity of cortical actin patches
S000000439	YBR235W	VHC1	Vacuolar protein Homologous to CCC family	686901	690263	1	Vacuolar membrane cation-chloride cotransporter (CCC)	Vacuolar membrane cation-chloride cotransporter (CCC); involved in potassium homeostasis and salt tolerance; localizes to sites of contact between vacuole and mitochondria (vCLAMPs); targeted to vacuole via AP-3 pathway; similar to mammalian electroneutral Na(+)-(K+)-C1- cotransporter family
S000000440	YBR236C	ABD1	""	690383	691693	-1	Methyltransferase	Methyltransferase; catalyzes the transfer of a methyl group from S-adenosylmethionine to the GpppN terminus of capped mRNA; nuclear protein that relocalizes to the cytosol in response to hypoxia
S000000441	YBR237W	PRP5	Pre-mRNA Processing	691969	694518	1	RNA helicase in the DEAD-box family	RNA helicase in the DEAD-box family; necessary for prespliceosome formation, bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA
S000000442	YBR238C	""	""	695107	697302	-1	Mitochondrial membrane protein	Mitochondrial membrane protein; not required for respiratory growth but causes a synthetic respiratory defect in combination with rmd9 mutations; transcriptionally up-regulated by TOR; deletion increases life span; YBR238C has a paralog, RMD9, that arose from the whole genome duplication
S000000443	YBR239C	ERT1	Ethanol Regulated Transcription factor	698354	699943	-1	Transcriptional regulator	Transcriptional regulator; involved in regulation of gluconeogenesis and fermentable carbon utilization; GFP-fusion protein localizes to cytoplasm, nucleus; null mutation affects periodicity of transcriptional and metabolic oscillation; plays role in restricting Ty1 transposition; member of the zinc cluster family of proteins, similar to Rds2p
S000000444	YBR240C	THI2	THIamine metabolism	700490	701842	-1	Transcriptional activator of thiamine biosynthetic genes	Transcriptional activator of thiamine biosynthetic genes; interacts with regulatory factor Thi3p to control expression of thiamine biosynthetic genes with respect to thiamine availability; acts together with Pdc2p to respond to thiaminediphosphate demand, possibly as related to carbon source availability; zinc finger protein of the Zn(II)2Cys6 type
S000000445	YBR241C	VVS1	Vacuolar protein Vital for Stress response	702589	704055	-1	Putative transporter, member of the sugar porter family	Putative transporter, member of the sugar porter family; localizes to the vacuolar membrane; non-essential gene; upregulated by and required for tolerance to 4-Methylcyclohexane methanol; YBR241C has a paralog, VPS73, that arose from the whole genome duplication 
S000000446	YBR242W	""	""	704670	705386	1	5'-deoxynucleotidase involved in deoxyribonucleoside monophosphate deg	5'-deoxynucleotidase involved in deoxyribonucleoside monophosphate degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; non-essential gene; YBR242W has a paralog, YGL101W, that arose from the whole genome duplication
S000000447	YBR243C	ALG7	Asparagine-Linked Glycosylation	705447	706793	-1	UDP-N-acetyl-glucosamine-1-P transferase	UDP-N-acetyl-glucosamine-1-P transferase; transfers Glc-Nac-P from UDP-GlcNac to Dol-P in the ER in the first step of the dolichol pathway of protein asparagine-linked glycosylation; inhibited by tunicamycin; human homolog DPAGT1 can complement yeast ALG7 mutant
S000000448	YBR244W	GPX2	Glutathione PeroXidase	707528	708016	1	Phospholipid hydroperoxide glutathione peroxidase	Phospholipid hydroperoxide glutathione peroxidase; late-acting antioxidant system that decreases mitochondrial ROS production and preserves electron transport chain (ETC) function; protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress; induced by glucose starvation; protein abundance increases in response to DNA replication stress
S000000449	YBR245C	ISW1	Imitation SWitch subfamily	708150	711539	-1	ATPase subunit of imitation-switch (ISWI) class chromatin remodelers	ATPase subunit of imitation-switch (ISWI) class chromatin remodelers; with Ioc3p forms Isw1a complex involved in repression of transcription initiation; with Ioc2p and Ioc4p forms Isw1b complex involved in regulation of transcription elongation; Isw1b recruited to ORFs by H3K36 methylation and acts with Chd1p to prevent trans-histone exchange over coding regions; binds to 3'UTR of HAC1 mRNA and limits its nuclear export attenuating the UPR
S000000450	YBR246W	RRT2	Regulator of rDNA Transcription	711591	712754	1	Methylesterase performing penultimate step of diphthamide biosynthesis	Methylesterase performing penultimate step of diphthamide biosynthesis; hydrolyzes methylated diphthine to produce diphthine and allows Dph6-catalyzed amidation reaction to occur; deletion leads to resistance to sordarin and accumulation of methylatediphthine; WD40 domain-containing protein; involved in endosomal recycling; forms complex with Rtt10p that functions in retromer-mediated pathway for recycling internalized cell-surface proteins
S000000451	YBR247C	ENP1	Essential Nuclear Protein	713004	714455	-1	Protein associated with U3 and U14 snoRNAs	Protein associated with U3 and U14 snoRNAs; required for pre-rRNA processing and 40S ribosomal subunit synthesis; localized in the nucleus and concentrated in the nucleolus; human BYSL functionally complements the heat sensitivity of an enp1 ts mutant
S000000452	YBR248C	HIS7	HIStidine requiring	714807	716465	-1	Imidazole glycerol phosphate synthase	Imidazole glycerol phosphate synthase; glutamine amidotransferase:cyclase that catalyzes the fifth step of histidine biosynthesis and also produces 5-aminoimidazole-4-carboxamide ribotide (AICAR), a purine precursor
S000000453	YBR249C	ARO4	AROmatic amino acid requiring	716882	717994	-1	3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase	3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase; catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by tyrosine or high concentrations of phenylalanine or tryptophan; relative distribution to the nucleus increases upon DNA replication stress
S000000454	YBR250W	SPO23	SPOrulation	719033	720604	1	Protein of unknown function	Protein of unknown function; associates with meiosis-specific protein Spo1p
S000000455	YBR251W	MRPS5	Mitochondrial Ribosomal Protein, Small subunit	721390	722313	1	Mitochondrial ribosomal protein of the small subunit	Mitochondrial ribosomal protein of the small subunit
S000000456	YBR252W	DUT1	DUTp pyrophosphatase	722611	723054	1	Deoxyuridine triphosphate diphosphatase (dUTPase)	Deoxyuridine triphosphate diphosphatase (dUTPase); catalyzes hydrolysis of dUTP to dUMP and PPi, thereby preventing incorporation of uracil into DNA during replication; critical for the maintenance of genetic stability; also has diphosphatase activity on deoxyinosine triphosphate; human homolog DUT allows growth of yeast haploid dut1 null mutant after sporulation of heterozygous diploid
S000000458	YBR254C	TRS20	TRapp Subunit	723736	724263	-1	Core component of transport protein particle (TRAPP) complexes I-III	Core component of transport protein particle (TRAPP) complexes I-III; TRAPPs are multimeric guanine nucleotide-exchange factors for GTPase Ypt1p, regulating ER-Golgi traffic (TRAPPI), intra-Golgi traffic (TRAPPII), endosome-Golgi traffic (TRAPPII and III) and autophagy (TRAPPIII); mutation leads to defects in endocytic recycling, block in sporulation/meiosis; mutations in human homolog TRAPPC2 cause spondyloepiphyseal dysplasia tarda, TRAPPC2 can complement yeast null mutant
S000000459	YBR255W	MTC4	Maintenance of Telomere Capping	724456	726540	1	Protein of unknown function	Protein of unknown function; required for normal growth rate at 15 degrees C; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; mtc4 is synthetically sick with cdc13-1
S000000460	YBR256C	RIB5	RIBoflavin biosynthesis	727386	728102	-1	Riboflavin synthase	Riboflavin synthase; catalyzes the last step of the riboflavin biosynthesis pathway
S000000461	YBR257W	POP4	Processing Of Precursor RNAs	728885	729724	1	Subunit of both RNase MRP and nuclear RNase P	Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
S000000462	YBR258C	SHG1	Set1c, Hypothetical G	729734	730162	-1	Subunit of the COMPASS (Set1C) complex	Subunit of the COMPASS (Set1C) complex; COMPASS methylates histone H3 on lysine 4 and is required in transcriptional silencing near telomeres
S000000463	YBR259W	""	""	730387	732453	1	Protein of unknown function	Protein of unknown function; YBR259W is not an essential gene; forms cytoplasmic foci upon DNA replication stress
S000000464	YBR260C	RGD1	Related GAP Domain	732639	734639	-1	GTPase-activating protein (RhoGAP) for Rho3p and Rho4p	GTPase-activating protein (RhoGAP) for Rho3p and Rho4p; involved in repolarization of actin cytoskeleton during adaptation to heat shock stress; phosphorylated by Pkc1p
S000000465	YBR261C	TAE1	Translation Associated Element	734832	735530	-1	AdoMet-dependent proline methyltransferase	AdoMet-dependent proline methyltransferase; catalyzes the dimethylation of ribosomal proteins Rpl12 and Rps25 at N-terminal proline residues; has a role in protein synthesis; fusion protein localizes to the cytoplasm
S000000466	YBR262C	MIC12	MItochondrial contact site and Cristae organizing system	735720	736040	-1	Component of the MICOS complex	Component of the MICOS complex; MICOS (formerly MINOS or MitOS) is a mitochondrial inner membrane complex that extends into the intermembrane space and has a role in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane; forms a subcomplex with Mic10p and Mic27p whose assembly and stability requires cardiolipin
S000000467	YBR263W	SHM1	Serine HydroxyMethyltransferase	736264	737736	1	Mitochondrial serine hydroxymethyltransferase	Mitochondrial serine hydroxymethyltransferase; converts serine to glycine plus 5,10 methylenetetrahydrofolate; involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis; reverse reaction generates serine
S000000468	YBR264C	YPT10	Yeast Protein Two	737770	738369	-1	Rab family GTP-binding protein	Rab family GTP-binding protein; contains the PEST signal sequence specific for proteolytic enzymes; may be involved in vesicular transport; overexpression leads to accumulation of Golgi-like cisternae with budding vesicles
S000000469	YBR265W	TSC10	Temperature-sensitive Suppressors of Csg2 mutants	738582	739544	1	3-ketosphinganine reductase	3-ketosphinganine reductase; catalyzes the second step in phytosphingosine synthesis; essential for growth in the absence of exogenous dihydrosphingosine or phytosphingosine; localized to lipid droplets; member of short chain dehydrogenase/reductase protein family; mutations in human homolog KDSR cause recessive progressive symmetric erythrokeratoderma
S000000470	YBR266C	SLM6	Synthetic Lethal with Mss4	739936	740388	-1	Protein with potential role in actin cytoskeleton organization	Protein with potential role in actin cytoskeleton organization; null mutant is slow-growing, sensitive to Congo Red, impaired in endocytosis, has increased lifespan; gene exhibits synthetic genetic interaction with MSS4 encoding phosphatidylinositol 4-phosphate kinase
S000000471	YBR267W	REI1	REquired for Isotropic bud growth	739841	741022	1	Cytoplasmic pre-60S factor	Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network
S000000472	YBR268W	MRPL37	Mitochondrial Ribosomal Protein, Large subunit	741299	741616	1	Mitochondrial ribosomal protein of the large subunit	Mitochondrial ribosomal protein of the large subunit
S000000473	YBR269C	SDH8	Succinate DeHydrogenase	742160	742576	-1	Protein required for assembly of succinate dehydrogenase	Protein required for assembly of succinate dehydrogenase; interacts with flavinylated Sdh1p and may function as a chaperone for free Sdh1p, protecting its FAD cofactor from redox reactions before assembly of the complex; soluble protein of the mitochondrial matrix; respiratory defect of null mutant is functionally complemented by Drosophila and human orthologs
S000000474	YBR270C	BIT2	Binding partner of Tor2p	742761	744398	-1	Subunit of TORC2, a regulator of plasma membrane (PM) homeostasis	Subunit of TORC2, a regulator of plasma membrane (PM) homeostasis; TORC2 also regulates actin cytoskeletal dynamics during polarized growth and cell wall integrity; interacts with Slm1p and Slm2p, homologous PH domain-containing TORC2 substrates; BIT2 has a paralog, BIT61, that arose from the whole genome duplication
S000000475	YBR271W	EFM2	Elongation Factor Methyltransferase	744852	746111	1	S-adenosylmethionine-dependent methyltransferase	S-adenosylmethionine-dependent methyltransferase; seven-beta-strand lysine methyltransferase which dimethylates translation elongation factor EF2 (Eft1p and Eft2p) at lysine 613 and methylates EF3 (Yef3p) at lysine 187; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; involved in regulation of translational termination; predicted involvement in ribosome biogenesis
S000000476	YBR272C	HSM3	enHanced Spontaneous Mutability	746361	747803	-1	Evolutionarily conserved 19S regulatory particle assembly-chaperone	Evolutionarily conserved 19S regulatory particle assembly-chaperone; involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); involved in DNA mismatch repair during slow growth; weak similarity to Msh1p; structural study suggests Hsm3p is a scaffold protein for Rpt1p-Rpt2p complex formation; ortholog of human 19S subunit S5b
S000000477	YBR273C	UBX7	UBiquitin regulatory X	748061	749371	-1	UBX (ubiquitin regulatory X) domain-containing protein	UBX (ubiquitin regulatory X) domain-containing protein; interacts with Cdc48p; UBX7 has a paralog, UBX6, that arose from the whole genome duplication
S000000478	YBR274W	CHK1	CHeckpoint Kinase	749594	751177	1	Serine/threonine kinase and DNA damage checkpoint effector	Serine/threonine kinase and DNA damage checkpoint effector; mediates cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase
S000000479	YBR275C	RIF1	RAP1-Interacting Factor	751356	757106	-1	Protein involved in control of DNA replication	Protein involved in control of DNA replication; binds Rap1p C-terminus, telomeres, centromeres, replication origins; contributes to resection of DNA double strand breaks (DSBs); acts synergistically with Rif2p to help control telomere length and establish telomeric silencing; in addition to controlling DNA replication initiation, plays ongoing role after initiation and controls events at blocked replication forks
S000000480	YBR276C	PPS1	Protein Phosphatase S phase	757620	760043	-1	Protein phosphatase	Protein phosphatase; has specificity for serine, threonine, and tyrosine residues; has a role in the DNA synthesis phase of the cell cycle
S000000481	YBR277C	""	""	760215	760616	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR278W
S000000482	YBR278W	DPB3	DNA Polymerase B (II) subunit	760294	760899	1	Third-largest subunit of DNA polymerase II (DNA polymerase epsilon)	Third-largest subunit of DNA polymerase II (DNA polymerase epsilon); required to maintain fidelity of chromosomal replication and also for inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; mRNA abundance peaks at the G1/S boundary of the cell cycle; DPB3 has a paralog, DLS1, that arose from the whole genome duplication
S000000483	YBR279W	PAF1	RNA Polymerase II Associated Factor	761257	762594	1	Component of the Paf1p complex involved in transcription elongation	Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cell cycle-regulated genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; homolog of human PD2/hPAF1
S000000484	YBR280C	SAF1	SCF Associated Factor	762784	764697	-1	F-Box protein involved in proteasome-dependent degradation of Aah1p	F-Box protein involved in proteasome-dependent degradation of Aah1p; involved in proteasome-dependent degradation of Aah1p during entry of cells into quiescence; interacts with Skp1
S000000485	YBR281C	DUG2	Deficient in Utilization of Glutathione	764970	767606	-1	Component of glutamine amidotransferase (GATase II)	Component of glutamine amidotransferase (GATase II); forms a complex with Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p)
S000000486	YBR282W	MRPL27	Mitochondrial Ribosomal Protein, Large subunit	768240	768680	1	Mitochondrial ribosomal protein of the large subunit	Mitochondrial ribosomal protein of the large subunit; homolog of human Bcl-2 interacting protein BMRP
S000000487	YBR283C	SSH1	Sec Sixty-one Homolog	768943	770415	-1	Subunit of the Ssh1 translocon complex	Subunit of the Ssh1 translocon complex; Sec61p homolog involved in co-translational pathway of protein translocation; not essential
S000000488	YBR284W	""	""	771239	773632	1	Putative metallo-dependent hydrolase superfamily protein	Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; null mutant exhibits longer telomeres, altered Ty mobility, decreased resistance to rapamycin and wortmannin; induced in response to hydrostatic pressure; not an essential gene; YBR284W has a paralog, YJL070C, that arose from the whole genome duplication
S000000489	YBR285W	""	""	773922	774356	1	Putative protein of unknown function	Putative protein of unknown function; YBR285W is not an essential gene
S000000490	YBR286W	APE3	AminoPEptidase	774700	776313	1	Vacuolar aminopeptidase Y	Vacuolar aminopeptidase Y; processed to mature form by Prb1p; targeted to vacuole via Vps10p-dependent endosomal vacuolar protein sorting pathway
S000000491	YBR287W	""	""	776571	777854	1	Protein of unknown function	Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the ER; YBR287W is not an essential gene
S000000492	YBR288C	APM3	clathrin Adaptor Protein complex Medium chain	778012	779463	-1	Mu3-like subunit of the clathrin associated protein complex (AP-3)	Mu3-like subunit of the clathrin associated protein complex (AP-3); functions in transport of alkaline phosphatase to the vacuole via the alternate pathway
S000000493	YBR289W	SNF5	Sucrose NonFermenting	779667	782384	1	Subunit of the SWI/SNF chromatin remodeling complex	Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf6p; relocates to the cytosol under hypoxic conditions
S000000494	YBR290W	BSD2	Bypass Sod1p Defects	782591	783556	1	Heavy metal ion homeostasis protein	Heavy metal ion homeostasis protein; facilitates trafficking of Smf1p and Smf2p metal transporters to vacuole where they are degraded; acts as an adaptor protein with Rsp5p in the regulated endocytosis of Smf1p and is itself ubiquitylated by Rsp5p; controls metal ion transport, prevents metal hyperaccumulation, functions in copper detoxification
S000000495	YBR291C	CTP1	Citrate Transport Protein	783673	784572	-1	Mitochondrial inner membrane citrate transporter	Mitochondrial inner membrane citrate transporter; member of the mitochondrial carrier family
S000000496	YBR292C	""	""	784702	785073	-1	Putative protein of unknown function	Putative protein of unknown function; conserved across S. cerevisiae strains; YBR292C is not an essential gene
S000000497	YBR293W	VBA2	Vacuolar Basic Amino acid transporter	787006	788430	1	Permease of basic amino acids in the vacuolar membrane	Permease of basic amino acids in the vacuolar membrane
S000000498	YBR294W	SUL1	SULfate	789235	791814	1	High affinity sulfate permease of the SulP anion transporter family	High affinity sulfate permease of the SulP anion transporter family; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates
S000000499	YBR295W	PCA1	P-type Cation-transporting ATPase	792849	796499	1	Cadmium transporting P-type ATPase	Cadmium transporting P-type ATPase; may also have a role in copper and iron homeostasis; stabilized by Cd binding, which prevents ubiquitination; S288C and other lab strains contain a G970R mutation which eliminates Cd transport function
S000000500	YBR296C	PHO89	PHOsphate metabolism	796798	798522	-1	Plasma membrane Na+/Pi cotransporter	Plasma membrane Na+/Pi cotransporter; active in early growth phase; similar to phosphate transporters of Neurospora crassa; transcription regulated by inorganic phosphate concentrations and Pho4p; mutations in related human transporter genes hPit1 and hPit2 are associated with hyperphosphatemia-induced calcification of vascular tissue and familial idiopathic basal ganglia calcification
S000000501	YBR297W	MAL33	MALtose	800523	801929	1	MAL-activator protein	MAL-activator protein; part of complex locus MAL3; nonfunctional in genomic reference strain S288C
S000000502	YBR298C	MAL31	MALtose fermentation	802631	804475	-1	Maltose permease	Maltose permease; high-affinity maltose transporter (alpha-glucoside transporter); encoded in the MAL3 complex locus; member of the 12 transmembrane domain superfamily of sugar transporters; functional in genomic reference strain S288C
S000000503	YBR299W	MAL32	MALtose	805351	807105	1	Maltase (alpha-D-glucosidase)	Maltase (alpha-D-glucosidase); inducible protein involved in maltose catabolism; encoded in the MAL3 complex locus; functional in genomic reference strain S288C; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose
S000000504	YBR300C	""	""	808600	809097	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YBR301W; YBR300C is not an essential gene
S000000505	YBR301W	PAU24	seriPAUperin family	809057	809419	1	Cell wall mannoprotein	Cell wall mannoprotein; has similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth
S000000507	YCL001W	RER1	Retention in the Endoplasmic Reticulum	111916	112482	1	Protein involved in retention of membrane proteins	Protein involved in retention of membrane proteins; including Sec12p, in the ER; localized to Golgi; functions as a retrieval receptor in returning membrane proteins to the ER
S000000508	YCL002C	""	""	110808	111675	-1	Putative protein of unknown function	Putative protein of unknown function; YCL002C is not an essential gene
S000000510	YCL004W	PGS1	PhosphatidylGlycerolphosphate Synthase	109105	110670	1	Phosphatidylglycerolphosphate synthase	Phosphatidylglycerolphosphate synthase; catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis
S000000511	YCL005W	LDB16	Low Dye Binding	108021	108791	1	Protein involved in lipid droplet (LD) assembly	Protein involved in lipid droplet (LD) assembly; forms a complex with Sei1p at ER-LD contact sites, stabilizing contact sites; ensures that LDs bud from the ER towards the cytosolic side of the membrane; null mutants have decreased net negative cell surface charge and localized accumulation of phosphatidic acid (PA) marker proteins; GFP-fusion protein expression is induced in response to MMS; null mutant can be complemented by the human seipin, BSCL2
S000000512	YCL006C	""	""	107240	107569	-1	Deleted ORF	Deleted ORF; does not encode a protein; this ORF was removed when a sequence update created a stop codon after residue 23
S000000513	YCL007C	""	""	106974	107366	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps verified ORF YCL005W-A; mutations in YCL007C were thought to confer sensitivity to calcofluor white, but this phenotype was later shown to be due to the defect in YCL005W-A
S000000514	YCL008C	STP22	STerile Pseudoreversion	105696	106853	-1	Component of the ESCRT-I complex	Component of the ESCRT-I complex; ESCRT-I is involved in ubiquitin-dependent sorting of proteins into the endosome; prevents polyubiquitination of the arrestin-related protein Rim8p, thereby directing its monoubiquitination by Rsp5p; homologous to the mouse and human Tsg101 tumor susceptibility gene; mutants exhibit a Class E Vps phenotype;
S000000515	YCL009C	ILV6	IsoLeucine Valine	104619	105548	-1	Regulatory subunit of acetolactate synthase	Regulatory subunit of acetolactate synthase; acetolactate synthase catalyzes the first step of branched-chain amino acid biosynthesis; enhances activity of the Ilv2p catalytic subunit, localizes to mitochondria
S000000516	YCL010C	SGF29	SaGa associated Factor	103571	104350	-1	Component of the HAT/Core module of the SAGA, SLIK, and ADA complexes	Component of the HAT/Core module of the SAGA, SLIK, and ADA complexes; HAT/Core module also contains Gcn5p, Ngg1p, and Ada2p; binds methylated histone H3K4; involved in transcriptional regulation through SAGA and TBP recruitment to target promoters and H3 acetylation
S000000517	YCL011C	GBP2	G-strand Binding Protein	102075	103358	-1	Poly(A+) RNA-binding protein involved in translation repression	Poly(A+) RNA-binding protein involved in translation repression; key surveillance factor for the selective export of spliced mRNAs from the nucleus to the cytoplasm; preference for intron-containing genes; similar to Npl3p; also binds single-stranded telomeric repeat sequence in vitro; relocalizes to the cytosol in response to hypoxia; GBP2 has a paralog, HRB1, that arose from the whole genome duplication
S000000518	YCL012W	""	""	100113	100808	1	Merged open reading frame	Merged open reading frame; does not encode a discrete protein; YCL012W was originally annotated as an independent ORF, but as a result of a sequence change, it was merged with an adjacent ORF into a single reading frame, designated YCL014W
S000000519	YCL013W	""	""	99600	99998	1	Deleted ORF	Deleted ORF; originally included in genome annotation but deleted due to sequence correction
S000000520	YCL014W	BUD3	BUD site selection	96281	101191	1	Guanine nucleotide exchange factor (GEF) for Cdc42p	Guanine nucleotide exchange factor (GEF) for Cdc42p; activates Cdc42p in early G1, accounting for the first stage of biphasic activation, with Cdc24p accounting for the second stage in late G1; involved in the Cdc42p-mediated assembly of the axial landmark that dictates the site for the next round of budding, resulting in the axial budding pattern in haploids; localizes with septins to the bud neck contractile ring in mitosis
S000000521	YCL016C	DCC1	Defective in sister Chromatid Cohesion	94621	95763	-1	Subunit of a complex with Ctf8p and Ctf18p	Subunit of a complex with Ctf8p and Ctf18p; shares some components with Replication Factor C; required for sister chromatid cohesion and telomere length maintenance
S000000522	YCL017C	NFS1	NiFS-like	92777	94270	-1	Cysteine desulfurase involved in iron-sulfur cluster biogenesis	Cysteine desulfurase involved in iron-sulfur cluster biogenesis; required for thio-modification of mitochondrial and cytoplasmic tRNAs; involved in DNA damage response by providing iron-sulfur cluster cofactors for DNA repair enzymes; essential protein located predominantly in mitochondria
S000000527	YCL022C	""	""	81570	82085	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps verified gene KCC4/YCL024W
S000000528	YCL023C	""	""	78949	79296	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified ORF KCC4
S000000529	YCL024W	KCC4	""	79162	82275	1	Protein kinase of the bud neck involved in the septin checkpoint	Protein kinase of the bud neck involved in the septin checkpoint; associates with septin proteins, negatively regulates Swe1p by phosphorylation, shows structural homology to bud neck kinases Gin4p and Hsl1p; KCC4 has a paralog, GIN4, that arose from the whole genome duplication
S000000530	YCL025C	AGP1	high-Affinity Glutamine Permease	76018	77919	-1	Broad-specificity, low-affinity amino acid permease	Broad-specificity, low-affinity amino acid permease; minor serine permease with major contributions from paralog Gnp1p; involved in uptake of asparagine, glutamine, and other amino acids; expression induced by amino acids and regulated by SPS plasma membrane amino acid sensor system (Ssy1p-Ptr3p-Ssy5p)
S000000531	YCL026C	""	""	74686	75045	-1	Deleted ORF	Deleted ORF; does not encode a protein; included in the original annotation of Chromosome III but later deleted due to sequence corrections
S000000532	YCL027W	FUS1	cell FUSion	71803	73341	1	Membrane protein localized to the shmoo tip	Membrane protein localized to the shmoo tip; required for cell fusion; expression regulated by mating pheromone; proposed to coordinate signaling, fusion, and polarization events required for fusion; potential Cdc28p substrate
S000000533	YCL028W	RNQ1	Rich in asparagine (N) and glutamine (Q)	70150	71367	1	[PIN(+)] prion, named for [PSI+] INducibility	[PIN(+)] prion, named for [PSI+] INducibility; an infectious protein conformation that is generally an ordered protein aggregate
S000000534	YCL029C	BIK1	BIlateral Karyogamy defect	68599	69921	-1	Microtubule-associated protein	Microtubule-associated protein; component of the interface between microtubules and the kinetochore; involved in sister chromatid separation and mitotic spindle elongation; involved in karyogamy during mating; stabilizes Kip2p at microtubule plus ends and recruits Kip2p to mitotic SPBs; essential in polyploid cells but not in haploid or diploid cells; promotes plus-end directed movement of Dyn1p; ortholog of mammalian CLIP-170
S000000535	YCL030C	HIS4	HIStidine requiring	65934	68333	-1	Multifunctional enzyme containing phosphoribosyl-ATP pyrophosphatase	Multifunctional enzyme containing phosphoribosyl-ATP pyrophosphatase; phosphoribosyl-AMP cyclohydrolase, and histidinol dehydrogenase activities; catalyzes the second, third, ninth and tenth steps in histidine biosynthesis
S000000536	YCL031C	RRP7	Ribosomal RNA Processing	64675	65568	-1	Essential protein involved in rRNA processing and ribosome biogenesis	Essential protein involved in rRNA processing and ribosome biogenesis; forms a complex with Utp22p; protein abundance increases in response to DNA replication stress
S000000537	YCL032W	STE50	STErile	63441	64481	1	Adaptor protein for various signaling pathways	Adaptor protein for various signaling pathways; involved in the mating response, invasive/filamentous growth and osmotolerance; acts as an adaptor that links the G protein-associated Cdc42p-Ste20p complex to the effector Ste11p to modulate signal transduction; localizes to cortical patches at the site of shmoo initiation, facilitating polarity establishment, and remaining at the shmoo tip during mating
S000000538	YCL033C	MXR2	peptide Methionine sulfoXide Reductase	62776	63282	-1	Methionine-R-sulfoxide reductase	Methionine-R-sulfoxide reductase; involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR1; involved in the regulation of lifespan
S000000539	YCL034W	LSB5	Las Seventeen Binding protein	61658	62722	1	Protein involved in membrane-trafficking events at plasma membrane	Protein involved in membrane-trafficking events at plasma membrane; interacts with actin regulators Sla1p and Las17p, ubiquitin, Arf3p to couple actin dynamics to membrane trafficking processes; similar structure to GGA family of proteins with N-terminal VHS domain and GAT domain; binds Las17p, which is homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly, actin polymerization; may mediate disassembly of Pan1 complex from endocytic coat
S000000540	YCL035C	GRX1	GlutaRedoXin	60841	61173	-1	Glutathione-dependent disulfide oxidoreductase	Glutathione-dependent disulfide oxidoreductase; hydroperoxide and superoxide-radical responsive, heat-stable, with active site cysteine pair; protects cells from oxidative damage; GRX1 has a paralog, GRX2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
S000000541	YCL036W	GFD2	Great for Full DEAD box protein activity	59026	60726	1	Protein of unknown function	Protein of unknown function; identified as a high-copy suppressor of a dbp5 mutation; GFD2 has a paralog, YDR514C, that arose from the whole genome duplication
S000000542	YCL037C	SRO9	Suppressor of rho3	57374	58678	-1	Cytoplasmic RNA-binding protein	Cytoplasmic RNA-binding protein; shuttles between nucleus and cytoplasm and is exported from the nucleus in an mRNA export-dependent manner; associates with translating ribosomes; involved in heme regulation of Hap1p as a component of the HMC complex, also involved in the organization of actin filaments; contains a La motif; role in preventing L-A mycovirus pathogenesis; SRO9 has a paralog, SLF1, that arose from the whole genome duplication
S000000543	YCL038C	ATG22	AuTophaGy related	54941	56527	-1	Vacuolar membrane protein	Vacuolar membrane protein; required for efflux of amino acids during autophagic body breakdown in vacuole; targeted to vacuole via AP-3 pathway; null mutation causes gradual loss of viability during starvation
S000000544	YCL039W	GID7	Glucose Induced Degradation deficient	52645	54882	1	Subunit of GID Complex that binds directly to central component Vid30p	Subunit of GID Complex that binds directly to central component Vid30p; GID complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; Gid7p contains six WD40 repeats; localizes to peroxisomes in oleate-growing cells; computational analysis suggests that Gid7p and Moh1p have similar functions
S000000545	YCL040W	GLK1	GLucoKinase	50838	52340	1	Glucokinase	Glucokinase; catalyzes the phosphorylation of glucose at C6 in the first irreversible step of glucose metabolism; one of three glucose phosphorylating enzymes; expression regulated by non-fermentable carbon sources; GLK1 has a paralog, EMI2, that arose from the whole genome duplication
S000000546	YCL041C	""	""	50133	50627	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps both the verified gene PDI1/YCL043C and the uncharacterized gene YCL042W
S000000547	YCL042W	""	""	50584	50943	1	Putative protein of unknown function	Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm
S000000548	YCL043C	PDI1	Protein Disulfide Isomerase	48653	50221	-1	Protein disulfide isomerase	Protein disulfide isomerase; multifunctional oxidoreductase of the ER lumen, essential for disulfide bond formation in secretory and cell-surface proteins, processing of non-native disulfide bonds; Ero1p activator; complexes with exomannosidase, Mnl1p to facilitate the recognition of misfolded glycoproteins and the trimming of glycan Man8GlcNAc2 to Man7GlcNAc2 on substrates, thereby accelerating ERAD; PDI1 has a paralog, EUG1, that arose from the whole genome duplication
S000000549	YCL044C	MGR1	Mitochondrial Genome Required	47111	48364	-1	Subunit of the mitochondrial (mt) i-AAA protease supercomplex	Subunit of the mitochondrial (mt) i-AAA protease supercomplex; i-AAA degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr3p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA
S000000550	YCL045C	EMC1	ER Membrane protein Complex	44623	46905	-1	Member of conserved endoplasmic reticulum membrane complex	Member of conserved endoplasmic reticulum membrane complex; involved in efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; interacts with Gal80p; homologous to worm H17B01.4/EMC-1, fly CG2943, and human KIAA0090
S000000551	YCL046W	""	""	46640	46963	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YCL045C
S000000552	YCL047C	POF1	Promoter Of Filamentation	43661	44437	-1	Nicotinamide mononucleotide-specific adenylyltransferase (NMNAT)	Nicotinamide mononucleotide-specific adenylyltransferase (NMNAT); catalyzes the conversion of nicotinamide mononucleotide (NMN) to nicotinamide adenine dinucleotide (NAD+); role in the nicotinamide riboside (NR) salvage pathway of NAD+ biosynthesis; involved in NR and NAD+ homeostasis; ATPase involved in protein quality control and filamentation pathways; interacts physically with Kss1p and suppresses the filamentation defect of a kss1 deletion
S000000553	YCL048W	SPS22	SPorulation Specific 2 too (2)	42165	43556	1	Protein of unknown function	Protein of unknown function; SPS22 has a paralog, SPS2, that arose from the whole genome duplication; redundant with Sps2p for the organization of the beta-glucan layer of the spore wall
S000000554	YCL049C	""	""	39786	40724	-1	Protein of unknown function	Protein of unknown function; localizes to membrane fraction; YCL049C is not an essential gene
S000000555	YCL050C	APA1	AP4A phosphorylase	37836	38801	-1	AP4A phosphorylase	AP4A phosphorylase; bifunctional diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase and ADP sulfurylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; catalyzes phosphorolysis of dinucleoside oligophosphates, cleaving substrates' alpha/beta-anhydride bond and introducing Pi into the beta-position of the corresponding NDP formed; protein abundance increases under DNA replication stress; APA1 has a paralog, APA2, that arose from the whole genome duplication
S000000556	YCL051W	LRE1	Laminarase REsistance	35865	37616	1	Protein involved in control of cell wall structure and stress response	Protein involved in control of cell wall structure and stress response; direct inhibitor of the nuclear Dbf2 related (NDR) kinase Cbk1p-Mob2p; overproduction confers resistance to cell-wall degrading enzymes; exhibits genetic interactions with genes involved in the cell wall integrity pathway; LRE1 has a paralog, HLR1, that arose from the whole genome duplication
S000000557	YCL052C	PBN1	Protease B Non-derepressible	34143	35393	-1	Component of glycosylphosphatidylinositol-mannosyltransferase I	Component of glycosylphosphatidylinositol-mannosyltransferase I; essential component; required for the autocatalytic post-translational processing of the protease B precursor Prb1p; localizes to ER in lumenal orientation; homolog of mammalian PIG-X
S000000559	YCL054W	SPB1	Suppressor of PaB1 mutant	31449	33974	1	AdoMet-dependent methyltransferase	AdoMet-dependent methyltransferase; involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants
S000000560	YCL055W	KAR4	KARyogamy	27929	28936	1	Transcription factor required for the mating response	Transcription factor required for the mating response; subunit of the mRNA m6A methyltransferase (MIS) complex, regulating meiosis at multiple steps, early prior to pre-meiotic S phase and late during the completion of meiosis and sporulation; exists in two forms, a slower-migrating form abundant during vegetative growth and a faster-migrating pheromone-induced form; null mutant exhibits abnormal lipid metabolic phenotype; homologous to human METTL14, a subunit of a m6A methyltransferase complex
S000000561	YCL056C	PEX34	PEroXin	26925	27359	-1	Protein that regulates peroxisome populations	Protein that regulates peroxisome populations; peroxisomal integral membrane protein; interacts with Pex11p, Pex25p, and Pex27p to control both constitutive peroxisome division and peroxisome morphology and abundance during peroxisome proliferation
S000000562	YCL057W	PRD1	PRoteinase yscD	24768	26906	1	Zinc metalloendopeptidase	Zinc metalloendopeptidase; found in the cytoplasm and intermembrane space of mitochondria; with Cym1p, involved in degradation of mitochondrial proteins and of presequence peptides cleaved from imported proteins; protein abundance increases in response to DNA replication stress
S000000563	YCL058C	FYV5	Function required for Yeast Viability	23523	23981	-1	De novo-originated gene from non-coding sequence	De novo-originated gene from non-coding sequence; protein can suppress mating and promote vegetative growth; shortens lag phase by physically interacting with Snf1p; binds with Matalpha2p to promoters of haploid-specific genes; functions in mating and fermentation, mediates crosstalk between reproduction and vegetative growth; required for survival upon exposure to K1 killer toxin; involved in ion homeostasis
S000000564	YCL059C	KRR1	contains KRR-R motif	22429	23379	-1	Nucleolar protein required for rRNA synthesis and ribosomal assembly	Nucleolar protein required for rRNA synthesis and ribosomal assembly; required for the synthesis of 18S rRNA and for the assembly of 40S ribosomal subunit; essential gene
S000000565	YCL060C	""	""	20409	21362	-1	Merged open reading frame	Merged open reading frame; does not encode a discrete protein; YCL060C was originally annotated as an independent ORF, but as a result of a sequence change, it was merged with an adjacent ORF into a single reading frame, designated YCL061C
S000000566	YCL061C	MRC1	Mediator of the Replication Checkpoint	18816	22106	-1	S-phase checkpoint protein required for DNA replication	S-phase checkpoint protein required for DNA replication; couples DNA helicase and polymerase; stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; defines a novel S-phase checkpoint with Hog1p that coordinates DNA replication and transcription upon osmostress; protects uncapped telomeres; Dia2p-dependent degradation mediates checkpoint recovery; exposure to ethanol affects localization; mammalian claspin homolog
S000000567	YCL062W	""	""	17955	18542	1	Merged open reading frame	Merged open reading frame; does not encode a discrete protein; YCL062W as originally annotated as an independent ORF, but as a result of sequence changes, it was merged with an adjacent ORF into a single reading frame, designated YCL063W
S000000568	YCL063W	VAC17	VACuole related	17290	18561	1	Phosphoprotein involved in vacuole inheritance	Phosphoprotein involved in vacuole inheritance; degraded in late M phase of the cell cycle; acts as a vacuole-specific receptor for myosin Myo2p; involved in regulation of asymmetric inheritance of aggregated/misfolded proteins and age reset
S000000569	YCL064C	CHA1	Catabolism of Hydroxy Amino acids	15798	16880	-1	Catabolic L-serine (L-threonine) deaminase	Catabolic L-serine (L-threonine) deaminase; catalyzes the degradation of both L-serine and L-threonine; required to use serine or threonine as the sole nitrogen source, transcriptionally induced by serine and threonine
S000000574	YCL069W	VBA3	Vacuolar Basic Amino acid transporter	9706	11082	1	Permease of basic amino acids in the vacuolar membrane	Permease of basic amino acids in the vacuolar membrane; VBA3 has a paralog, VBA5, that arose from a segmental duplication
S000000579	YCL074W	""	""	2824	3750	1	Pseudogene: encodes fragment of Ty Pol protein	Pseudogene: encodes fragment of Ty Pol protein
S000000580	YCL075W	""	""	2126	2566	1	Pseudogene: encodes fragment of Ty Pol protein	Pseudogene: encodes fragment of Ty Pol protein
S000000581	YCL076W	""	""	1392	2135	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000000589	YCL026C-A	FRM2	Fatty acid Repression Mutant	74704	75285	-1	Type II nitroreductase, using NADH as reductant	Type II nitroreductase, using NADH as reductant; mutants are defective in fatty acid mediated repression of genes involved in fatty acid biosynthesis indicative of a role in lipid signaling; involved in the oxidative stress response; transcription induction by cadmium and selenite indicates a possible role in the metal stress response; expression induced in cells treated with the mycotoxin patulin
S000000594	YCR001W	""	""	115685	115999	1	Putative protein of unknown function	Putative protein of unknown function; conserved among S. cerevisiae strains; YCR001W is not an essential gene
S000000595	YCR002C	CDC10	Cell Division Cycle	117380	118348	-1	Component of the septin ring, required for cytokinesis	Component of the septin ring, required for cytokinesis; septins are GTP-binding proteins that assemble into rod-like hetero-oligomers that can associate to form filaments; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells; N-terminus interacts with phosphatidylinositol-4,5-bisphosphate; protein abundance increases under DNA damage stress
S000000596	YCR003W	MRPL32	Mitochondrial Ribosomal Protein, Large subunit	118620	119171	1	Mitochondrial ribosomal protein of the large subunit	Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress
S000000597	YCR004C	YCP4	""	119575	120318	-1	Protein of unknown function	Protein of unknown function; has sequence and structural similarity to flavodoxins; predicted to be palmitoylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
S000000598	YCR005C	CIT2	CITrate synthase	120946	122328	-1	Citrate synthase, peroxisomal isozyme involved in glyoxylate cycle	Citrate synthase, peroxisomal isozyme involved in glyoxylate cycle; catalyzes condensation of acetyl coenzyme A and oxaloacetate to form citrate; expression is controlled by Rtg1p and Rtg2p transcription factors; SCF-Ucc1 regulates level of Cit2p to maintain citrate homeostasis; oxaloacetate-dependent positive feedback loop inhibits Cit2p ubiquitination; CIT2 has a paralog, CIT1, that arose from the whole genome duplication
S000000599	YCR006C	""	""	122530	123003	-1	Putative protein of unknown function	Putative protein of unknown function; conserved among S. cerevisiae strains; YCR006C is not an essential gene
S000000600	YCR007C	DFP3	DUP240 Family Protein	126011	126730	-1	Putative integral membrane protein	Putative integral membrane protein; member of DUP240 gene family; SWAT-GFP and mCherry fusion proteins localize to the cell periphery and vacuole; YCR007C is not an essential gene
S000000601	YCR008W	SAT4	""	128470	130281	1	Ser/Thr protein kinase involved in salt tolerance, nutrient response	Ser/Thr protein kinase involved in salt tolerance, nutrient response; functions in regulation of Trk1p-Trk2p potassium transporter; overexpression affects the Fe-S and lipoamide containing proteins in the mitochondrion; required for lipoylation of Lat1p, Kgd2p and Gcv3p; partially redundant with Hal5p; has similarity to Npr1p; localizes to the cytoplasm and mitochondrion
S000000602	YCR009C	RVS161	Reduced Viability on Starvation	130745	131542	-1	Amphiphysin-like lipid raft protein	Amphiphysin-like lipid raft protein; N-BAR domain protein that interacts with Rvs167p and regulates polarization of the actin cytoskeleton, endocytosis, cell polarity, cell fusion and viability following starvation or osmotic stress
S000000603	YCR010C	ADY2	Accumulation of DYads	132273	133124	-1	Acetate transporter required for normal sporulation	Acetate transporter required for normal sporulation; phosphorylated in mitochondria; ADY2 has a paralog, ATO2, that arose from the whole genome duplication
S000000604	YCR011C	ADP1	ATP-Dependent Permease	133724	136873	-1	Putative ATP-dependent permease of the ABC transporter family	Putative ATP-dependent permease of the ABC transporter family
S000000605	YCR012W	PGK1	3-PhosphoGlycerate Kinase	137746	138996	1	3-phosphoglycerate kinase	3-phosphoglycerate kinase; catalyzes transfer of high-energy phosphoryl groups from the acyl phosphate of 1,3-bisphosphoglycerate to ADP to produce ATP; key enzyme in glycolysis and gluconeogenesis
S000000607	YCR014C	POL4	POLymerase	139185	140933	-1	DNA polymerase IV	DNA polymerase IV; undergoes pair-wise interactions with Dnl4p-Lif1p and Rad27p to mediate repair of DNA double-strand breaks by non-homologous end joining (NHEJ); homologous to mammalian DNA polymerase beta
S000000608	YCR015C	CTO1	Cold TOlerance	141217	142170	-1	Protein required for cold tolerance	Protein required for cold tolerance; involved in phosphate uptake; YCR015C is not an essential gene
S000000609	YCR016W	RBP95	rRNA-binding protein helix H95	143634	144506	1	RNA-binding ribosome assembly factor	RNA-binding ribosome assembly factor; constituent of early pre-60S particles; genetically and physically linked to most NOP8 60s ribosome pre-assembly complex members and to ribosomal protein Rpl3p; contains two independent RNA-interacting domains; associates with helix H95 in 3' region of 25S rRNA, in close proximity to binding sites of Npa1p and Rpl3p; interacts with several snoRNAs
S000000610	YCR017C	CWH43	Calcofluor White Hypersensitive	144773	147634	-1	GPI lipid remodelase	GPI lipid remodelase; responsible for introducing ceramides into GPI anchors having a C26:0 fatty acid in sn-2 of the glycerol moiety; can also use lyso-GPI protein anchors and various base resistant lipids as substrates; contains 14-16 transmembrane segments and several putative glycosylation and phosphorylation sites; null mutation is synthetically lethal with pkc1 deletion
S000000612	YCR019W	MAK32	MAintenance of Killer	152837	153928	1	Protein necessary for stability of L-A dsRNA-containing particles	Protein necessary for stability of L-A dsRNA-containing particles
S000000613	YCR020C	PET18	PETite colonies	154012	154659	-1	Protein of unknown function	Protein of unknown function; has weak similarity to proteins involved in thiamin metabolism; expression is induced in the absence of thiamin
S000000614	YCR020C-A	MAK31	MAintenance of Killer	154830	155096	-1	Non-catalytic subunit of N-terminal acetyltransferase of the NatC type	Non-catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus; member of the Sm protein family
S000000615	YCR021C	HSP30	Heat Shock Protein	156109	157107	-1	Negative regulator of the H(+)-ATPase Pma1p	Negative regulator of the H(+)-ATPase Pma1p; stress-responsive protein; hydrophobic plasma membrane localized; induced by heat shock, ethanol treatment, weak organic acid, glucose limitation, and entry into stationary phase
S000000616	YCR022C	""	""	157421	157765	-1	Putative protein of unknown function	Putative protein of unknown function; conserved among S. cerevisiae strains; YCR022C is not an essential gene
S000000617	YCR023C	""	""	158538	160373	-1	Vacuolar membrane protein of unknown function	Vacuolar membrane protein of unknown function; targeted to vacuole via AP-3 pathway; member of multidrug resistance family; not an essential gene
S000000618	YCR024C	SLM5	Synthetic Lethal with Mss4	160744	162222	-1	Mitochondrial asparaginyl-tRNA synthetase	Mitochondrial asparaginyl-tRNA synthetase
S000000619	YCR024C-A	PMP1	Plasma Membrane Proteolipid	162945	163067	-1	Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p	Regulatory subunit for the plasma membrane H(+)-ATPase Pma1p; small single-membrane span proteolipid; forms unique helix and positively charged cytoplasmic domain that is able to specifically segregate phosphatidylserines; PMP1 has a paralog, PMP2, that arose from the whole genome duplication
S000000620	YCR025C	""	""	163446	163856	-1	Putative protein of unknown function	Putative protein of unknown function; conserved across S. cerevisiae strains; YCR025C is not an essential gene
S000000621	YCR026C	NPP1	ecto-Nucleotide Pyrophosphatase/Phosphodiesterase	164111	166339	-1	Nucleotide pyrophosphatase/phosphodiesterase	Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp2p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; involved in spore wall assembly; NPP1 has a paralog, NPP2, that arose from the whole genome duplication, and an npp1 npp2 double mutant exhibits reduced dityrosine fluorescence relative to the single mutants
S000000622	YCR027C	RHB1	RHeB homolog	167370	167999	-1	Putative Rheb-related GTPase	Putative Rheb-related GTPase; involved in regulating canavanine resistance and arginine uptake; member of the Ras superfamily of G-proteins
S000000623	YCR028C	FEN2	FENpropimorph resistance	170886	172424	-1	Plasma membrane H+-pantothenate symporter	Plasma membrane H+-pantothenate symporter; confers sensitivity to the antifungal agent fenpropimorph; relocalizes from vacuole to cytoplasm upon DNA replication stress
S000000626	YCR030C	SYP1	Suppressor of Yeast Profilin deletion	173826	176438	-1	Inhibitor of Las17p-mediated Arp2/3 complex activation	Inhibitor of Las17p-mediated Arp2/3 complex activation; F-BAR protein that directly inhibits Las17p stimulation of Arp2/3 complex-mediated actin assembly in vitro; involved in formation of the endocytic site; intrinsically disordered domain induces bundling of Cdc11p-capped septin filaments to promote septin collar assembly, similar to mammalian ortholog FCHo2; localizes to sites of polarized growth, the septin ring, and endocytic patches; involved in actin cytoskeletal organization
S000000627	YCR031C	RPS14A	Ribosomal Protein of the Small subunit	177500	178220	-1	Protein component of the small (40S) ribosomal subunit	Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14A has a paralog, RPS14B, that arose from the whole genome duplication
S000000628	YCR032W	BPH1	Beige Protein Homolog	179520	186023	1	Endosomal protein involved in endocytosis and autophagy	Endosomal protein involved in endocytosis and autophagy; homologous to Chediak-Higashi syndrome and Beige proteins, both of which are implicated in disease syndromes in human and mouse, respectively, due to defective lysosomal trafficking; mutant phenotype and genetic interactions suggest a role in protein sorting
S000000629	YCR033W	SNT1	SaNT domains	186489	190169	1	Set3C histone deacetylase scaffold	Set3C histone deacetylase scaffold; can act as prion [ESI+] (Expressed Subtelomeric Information) that drives emergence and transgenerational inheritance of activated chromatin state; prion conformer is triggered by transient Snt1p phosphorylation upon cell cycle arrest; [ESI+] reshapes the activity Set3C, recruiting RNAPII, interfering with Rap1p binding to activate genes in otherwise repressed subtelomeric domains; relocalizes to cytosol in response to hypoxia
S000000630	YCR034W	ELO2	fatty acid ELOngation	190592	191635	1	Fatty acid elongase, involved in sphingolipid biosynthesis	Fatty acid elongase, involved in sphingolipid biosynthesis; acts on fatty acids of up to 24 carbons in length; mutations have regulatory effects on 1,3-beta-glucan synthase, vacuolar ATPase, and the secretory pathway; ELO2 has a paralog, ELO1, that arose from the whole genome duplication; lethality of the elo2 elo3 double null mutation is functionally complemented by human ELOVL1 and weakly complemented by human ELOVL3 or ELOV7
S000000631	YCR035C	RRP43	Ribosomal RNA Processing	191834	193018	-1	Exosome non-catalytic core component	Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp43p (OIP2, EXOSC8); protein abundance increases in response to DNA replication stress
S000000632	YCR036W	RBK1	RiBoKinase	193297	194298	1	Ribokinase, phosphorylates ribose to ribose-5-phosphate	Ribokinase, phosphorylates ribose to ribose-5-phosphate; required for recycling ribose during nucleotide metabolism
S000000633	YCR037C	PHO87	PHOsphate metabolism	194414	197185	-1	Low-affinity inorganic phosphate (Pi) transporter	Low-affinity inorganic phosphate (Pi) transporter; acts upstream of Pho81p in regulation of PHO pathway; targeted to vacuole via AP-3 pathway; expression is independent of Pi concentration and Pho4p activity; contains 12 membrane-spanning segments; PHO87 has a paralog, PHO90, that arose from the whole genome duplication
S000000639	YCR043C	""	""	206261	206644	-1	Putative protein of unknown function	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi apparatus; YCR043C is not an essential gene
S000000640	YCR044C	PER1	protein Processing in the ER	206877	207950	-1	Protein of the endoplasmic reticulum	Protein of the endoplasmic reticulum; required for GPI-phospholipase A2 activity that remodels the GPI anchor as a prerequisite for association of GPI-anchored proteins with lipid rafts; functionally complemented by human ortholog PERLD1
S000000641	YCR045C	RRT12	Regulator of rDNA Transcription	208135	209610	-1	Probable subtilisin-family protease	Probable subtilisin-family protease; role in formation of the dityrosine layer of spore walls; localizes to the spore wall and also the nuclear envelope and ER region in mature spores
S000000642	YCR046C	IMG1	Integrity of Mitochondrial Genome	209914	210423	-1	Mitochondrial ribosomal protein of the large subunit	Mitochondrial ribosomal protein of the large subunit; required for respiration and for maintenance of the mitochondrial genome
S000000643	YCR047C	BUD23	BUD site selection	210718	211545	-1	Ribosome biogenesis factor	Ribosome biogenesis factor; methyltransferase that methylates residue G1575 of 18S rRNA; required for rRNA processing and nuclear export of 40S ribosomal subunits independently of methylation activity; required for recruitment of Rps2p; functions with DEAH-box RNA helicase Ecm16p; diploid mutant displays random budding pattern; functional homolog of human WBSCR22
S000000644	YCR048W	ARE1	Acyl-coenzyme A: cholesterol acyl transferase-Related Enzyme	211929	213761	1	Acyl-CoA:sterol acyltransferase	Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the absence of oxygen; ARE1 has a paralog, ARE2, that arose from the whole genome duplication
S000000645	YCR049C	""	""	211871	212317	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000000646	YCR050C	""	""	213464	213772	-1	Non-essential protein of unknown function	Non-essential protein of unknown function; deletion mutant is synthetically sick or lethal with alpha-synuclein
S000000647	YCR051W	""	""	214071	214739	1	Putative protein of unknown function	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; contains ankyrin (Ank) repeats; YCR051W is not an essential gene
S000000648	YCR052W	RSC6	Remodel the Structure of Chromatin	214994	216445	1	Component of the RSC chromatin remodeling complex	Component of the RSC chromatin remodeling complex; essential for mitotic growth; RSC6 has a paralog, SNF12, that arose from the whole genome duplication
S000000649	YCR053W	THR4	THReonine requiring	216697	218241	1	Threonine synthase	Threonine synthase; conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway
S000000650	YCR054C	CTR86	Copper TRansport protein	218376	220067	-1	Essential protein of unknown function	Essential protein of unknown function; with orthologs in Ashbya gossypii and Candida albicans; similar to human ATXN10, mutations in which cause spinocerebellar ataxia type 10; codon usage corresponds to that observed for yeast genes expressed at low levels; relative distribution to the nucleus increases upon DNA replication stress
S000000653	YCR057C	PWP2	Periodic tryptophan (W) Protein	220457	223228	-1	Conserved 90S pre-ribosomal component	Conserved 90S pre-ribosomal component; essential for proper endonucleolytic cleavage of the 35 S rRNA precursor at A0, A1, and A2 sites; contains eight WD-repeats; PWP2 deletion leads to defects in cell cycle and bud morphogenesis
S000000655	YCR059C	YIH1	Yeast Impact Homolog	223454	224230	-1	Negative regulator of eIF2 kinase Gcn2p	Negative regulator of eIF2 kinase Gcn2p; competes with Gcn2p for binding to Gcn1p; may contribute to regulation of translation in response to starvation via regulation of Gcn2p; binds to monomeric actin and to ribosomes and polyribosomes; ortholog of mammalian IMPACT
S000000656	YCR060W	TAH1	Tpr-containing protein Associated with Hsp90	224399	224734	1	Component of conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1)	Component of conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly of large protein complexes such as box C/D snoRNPs and RNA polymerase II; contains a single TPR domain with at least two TPR motifs; plays a role in determining prion variants
S000000657	YCR061W	TVS1	Transmembrane protein Vital for Stress response	225563	227458	1	Protein of unknown function	Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; non-essential gene; upregulated by and required for tolerance to 4-Methylcyclohexane methanol; induced by treatment with 8-methoxypsoralen and UVA irradiation; contains 10-11 predicted transmembrane domains 
S000000658	YCR062W	""	""	225819	226181	1	Merged open reading frame	Merged open reading frame; does not encode a discrete protein; YCR062W was originally annotated as an independent ORF, but as a result of a sequence change, it was merged with an adjacent ORF into a single reading frame, designated YCR061W
S000000659	YCR063W	BUD31	BUD site selection	228318	228791	1	Component of the SF3b subcomplex of the U2 snRNP	Component of the SF3b subcomplex of the U2 snRNP; increases efficiency of first and second step pre-mRNA splicing; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern; facilitates passage through G1/S Start, but is not required for G2/M transition or exit from mitosis
S000000661	YCR065W	HCM1	High-Copy suppressor of Calmodulin	229310	231004	1	Forkhead transcription factor	Forkhead transcription factor; drives S-phase activation of genes involved in chromosome segregation, spindle dynamics, budding; also activates genes involved in respiration, use of alternative energy sources, NAD synthesis, oxidative stress resistance; regulated by cell wall integrity checkpoint; key factor in early adaptation to nutrient deficiency and diauxic shift; suppressor of calmodulin mutants with specific SPB assembly defects; ortholog of C. elegans lifespan regulator PHA-4
S000000662	YCR066W	RAD18	RADiation sensitive	231500	232963	1	E3 ubiquitin ligase	E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA
S000000663	YCR067C	SED4	Suppressor of Erd2 Deletion	233125	236322	-1	Integral ER membrane protein that stimulates Sar1p GTPase activity	Integral ER membrane protein that stimulates Sar1p GTPase activity; involved in COPII vesicle budding through disassociation of coat proteins from membranes onto liposomes; binds Sec16p; SED4 has a paralog, SEC12, that arose from the whole genome duplication
S000000664	YCR068W	ATG15	AuTophaGy related	237214	238776	1	Phospholipase	Phospholipase; hydrolyses phosphatidylserine, with minor activity against cardiolipin and PtdEtn; required for lysis of autophagic and CVT bodies; targeted to the vacuole via the Pep1p-dependent endosomal VPS pathway; targeted to intravacuolar vesicles during autophagy by the MVB pathway; maintains lipid droplet quantity after the diauxic shift; regulates lipolysis; single membrane spanning protein with cytosolic N-term and the rest in the ER lumen; expression regulated by Yap1p during autophagy
S000000665	YCR069W	CPR4	Cyclosporin-sensitive Proline Rotamase	239055	240011	1	Peptidyl-prolyl cis-trans isomerase (cyclophilin)	Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; has a potential role in the secretory pathway; CPR4 has a paralog, CPR8, that arose from the whole genome duplication
S000000667	YCR071C	IMG2	Integrity of Mitochondrial Genome	240103	240543	-1	Mitochondrial ribosomal protein of the large subunit	Mitochondrial ribosomal protein of the large subunit; conserved in metazoa, with similarity to human mitochondrial ribosomal protein MRPL49
S000000668	YCR072C	RSA4	RiboSome Assembly	240805	242352	-1	Ribosomal large subunit (60S) assembly factor	Ribosomal large subunit (60S) assembly factor; association of the N-terminal ubiquitin-like (UBL) domain of this preribosomal factor with the MIDAS domain in the Rea1p tail, promoted by the GTPase Nog2p, is required for the ATP-dependent dissociation of preribosomal factors including the Rix1p subcomplex, Rea1p, Nog2p and Rsa1p itself prior to nuclear export of pre-60S particles; contains WD-repeats and a UBL domain; localizes to the nucleolus 
S000000669	YCR073C	SSK22	Suppressor of Sensor Kinase	242588	246583	-1	MAP kinase kinase kinase of HOG1 mitogen-activated signaling pathway	MAP kinase kinase kinase of HOG1 mitogen-activated signaling pathway; functionally redundant with Ssk2p; interacts with and is activated by Ssk1p; phosphorylates Pbs2p; SSK22 has a paralog, SSK2, that arose from the whole genome duplication
S000000671	YCR075C	ERS1	ERd Suppressor	248033	248815	-1	Protein involved in cystine transport	Protein involved in cystine transport; localizes to the vacuole, plasma membrane and endosome; similarity to human cystinosin, a H(+)-driven transporter involved in L-cystine export from lysosomes and implicated in the disease cystinosis; contains seven transmembrane domains; mutation is functionally complemented by human CTNS
S000000672	YCR076C	FUB1	FUnction of Boundary	249293	250045	-1	Proteasome-binding protein	Proteasome-binding protein; interacts physically with multiple subunits of the 20S proteasome and genetically with genes encoding 20S core particle and 19S regulatory particle subunits; specifically blocks all three proteasome active sites; exhibits boundary activity which inhibits the propagation of heterochromatic silencing; contains a PI31 proteasome regulator domain and sequence similarity with human PSMF1, a proteasome inhibitor; not an essential gene
S000000673	YCR077C	PAT1	Protein Associated with Topoisomerase II	250238	252628	-1	Deadenylation-dependent mRNA-decapping factor	Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; binds to mRNAs under glucose starvation, most often in the 3' UTR; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress; phosphorylation by PKA inhibits P body foci formation
S000000677	YCR081W	SRB8	Suppressor of RNA polymerase B	254371	258654	1	Subunit of the RNA polymerase II mediator complex	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression
S000000678	YCR082W	AHC2	Ada Histone acetyltransferase complex Component	258883	259269	1	Component of the ADA histone acetyltransferase complex	Component of the ADA histone acetyltransferase complex; Ahc2p and Ahc1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; may tether Ahc1p to the complex
S000000679	YCR083W	TRX3	ThioRedoXin	259578	259961	1	Mitochondrial thioredoxin	Mitochondrial thioredoxin; highly conserved oxidoreductase required to maintain the redox homeostasis of the cell, forms the mitochondrial thioredoxin system with Trr2p, redox state is maintained by both Trr2p and Glr1p
S000000680	YCR084C	TUP1	dTMP-UPtake	260311	262452	-1	General repressor of transcription in complex with Cyc8p	General repressor of transcription in complex with Cyc8p; involved in the establishment of repressive chromatin structure through interactions with histones H3 and H4 and stabilization of nucleosomes over promoters; appears to enhance expression of mating-related genes; functions as a coactivator in certain situations
S000000681	YCR085W	""	""	262916	263269	1	Putative protein of unknown function	Putative protein of unknown function; conserved among S. cerevisiae strains; YCR085W is not an essential gene
S000000682	YCR086W	CSM1	Chromosome Segregation in Meiosis	263392	263964	1	Nucleolar protein that mediates homolog segregation during meiosis I	Nucleolar protein that mediates homolog segregation during meiosis I; forms a complex with Lrs4p and then Mam1p at kinetochores; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation
S000000683	YCR087W	""	""	263976	264491	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized gene YCR087C-A; YCR087W is not an essential gene
S000000684	YCR088W	ABP1	Actin Binding Protein	265068	266846	1	Actin-binding protein of the cortical actin cytoskeleton	Actin-binding protein of the cortical actin cytoskeleton; important for activation of actin nucleation mediated by the Arp2/Arp3 complex; inhibits actin filament elongation at the barbed-end; phosphorylation within its proline-rich region, mediated by Cdc28p and Pho85p, protects Abp1p from PEST sequence-mediated proteolysis; mammalian homolog of HIP-55 (hematopoietic progenitor kinase 1 [HPK1]-interacting protein of 55 kDa)
S000000685	YCR089W	FIG2	Factor-Induced Gene	267434	272263	1	Cell wall adhesin, expressed specifically during mating	Cell wall adhesin, expressed specifically during mating; may be involved in maintenance of cell wall integrity during mating; FIG2 has a paralog, AGA1, that arose from the whole genome duplication
S000000686	YCR090C	""	""	272315	272863	-1	Putative protein of unknown function	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YCR090C is not an essential gene
S000000687	YCR091W	KIN82	protein KINase	274404	276566	1	Putative serine/threonine protein kinase	Putative serine/threonine protein kinase; implicated in the regulation of phospholipid asymmetry through the activation of phospholipid translocases (flippases); involved in the phosphorylation of upstream inhibitory kinase Ypk1p along with Fpk1p; has a redundant role in the cellular response to mating pheromone; KIN82 has a paralog, FPK1, that arose from the whole genome duplication
S000000688	YCR092C	MSH3	MutS Homolog	276764	279820	-1	Mismatch repair protein	Mismatch repair protein; forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability
S000000689	YCR093W	CDC39	Cell Division Cycle	280117	286443	1	Subunit of the CCR4-NOT1 core complex	Subunit of the CCR4-NOT1 core complex; this complex has multiple roles in the regulation of mRNA levels including regulation of transcription and destabilization of mRNA by deadenylation; basal transcription factor that increases initiation and elongation; activates the ATPase activity of Dhh1p, resulting in processing body disassembly
S000000690	YCR094W	CDC50	Cell Division Cycle	286762	287937	1	Endosomal protein that interacts with phospholipid flippase Drs2p	Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication
S000000691	YCR095C	OCA4	Oxidant-induced Cell-cycle Arrest	288170	289258	-1	Cytoplasmic protein required for replication of Brome mosaic virus	Cytoplasmic protein required for replication of Brome mosaic virus; S. cerevisiae is a model system for studying replication of positive-strand RNA viruses in their natural hosts
S000000695	YCR098C	GIT1	GlycerophosphoInosiTol	297049	298605	-1	Plasma membrane permease	Plasma membrane permease; mediates uptake of glycerophosphoinositol and glycerophosphocholine as sources of the nutrients inositol and phosphate; expression and transport rate are regulated by phosphate and inositol availability
S000000696	YCR099C	""	""	300832	301299	-1	Putative protein of unknown function	Putative protein of unknown function
S000000697	YCR100C	EMA35	Efficient Mitochondria targeting-Associated protein	301271	302221	-1	Protein involved in protein sorting	Protein involved in protein sorting; involved in targeting mitochondrial membrane proteins to mitochondrial translocation system; mutation causes respiration defects
S000000698	YCR101C	""	""	302482	303030	-1	Putative protein of unknown function	Putative protein of unknown function; localizes to the membrane fraction; YCR101C is not an essential gene
S000000699	YCR102C	""	""	304361	305467	-1	Putative quinone oxidoreductase	Putative quinone oxidoreductase; overexpression improves ethanol production and acid stress resistance; involved in copper metabolism; similar to Cochliobolus carbonum toxD gene
S000000702	YCR105W	ADH7	Alcohol DeHydrogenase	309070	310155	1	NADPH-dependent medium chain alcohol dehydrogenase	NADPH-dependent medium chain alcohol dehydrogenase; has broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance
S000000703	YCR106W	RDS1	Regulator of Drug Sensitivity	310958	313456	1	Putative zinc cluster transcription factor	Putative zinc cluster transcription factor; involved in conferring resistance to cycloheximide
S000000704	YCR107W	AAD3	Aryl-Alcohol Dehydrogenase	313890	314981	1	Putative aryl-alcohol dehydrogenase	Putative aryl-alcohol dehydrogenase; similar to P. chrysosporium aryl-alcohol dehydrogenase; mutational analysis has not yet revealed a physiological role; AAD3 has a paralog, AAD15, that arose from a segmental duplication; members of the AAD gene family comprise three pairs (AAD3 + AAD15, AAD6/AAD16 + AAD4, AAD10 + AAD14) whose two genes are more related to one another than to other members of the family
S000000718	YCR073W-A	SOL2	Suppressor Of Los1-1	246963	247910	1	Protein with a possible role in tRNA export	Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL2 has a paralog, SOL1, that arose from the whole genome duplication
S000000727	YEL001C	IRC22	Increased Recombination Centers	150301	150978	-1	Protein of unknown function	Protein of unknown function; green fluorescent protein (GFP)-fusion localizes to the ER; YEL001C is non-essential; null mutant displays increased levels of spontaneous Rad52p foci
S000000728	YEL002C	WBP1	Wheat germ agglutinin-Binding Protein	148722	150014	-1	Non-catalytic beta subunit of the oligosaccharyltransferase (OST) comp	Non-catalytic beta subunit of the oligosaccharyltransferase (OST) complex; the OST complex catalyzes N-linked glycosylation of newly synthesized proteins in the ER; forms a pocket with Ost2p involved in binding terminal glucose units of the donor glycan; human homolog DDOST can complement yeast growth defect during down-regulation of the yeast gene
S000000729	YEL003W	GIM4	Gene Involved in Microtubule biogenesis	148176	148599	1	Subunit of the heterohexameric cochaperone prefoldin complex	Subunit of the heterohexameric cochaperone prefoldin complex; complex binds specifically to cytosolic chaperonin and transfers target proteins to it
S000000730	YEL004W	YEA4	""	146951	147979	1	Uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) transporter	Uridine diphosphate-N-acetylglucosamine (UDP-GlcNAc) transporter; required for cell wall chitin synthesis; localized to the ER
S000000731	YEL005C	VAB2	VAc8p Binding	145907	146755	-1	Subunit of the BLOC-1 complex involved in endosomal maturation	Subunit of the BLOC-1 complex involved in endosomal maturation; interacts with Vps21p-GFP; has potential role in vacuolar function, as suggested by its ability to bind Vac8p; likely member of; Vab2p-GFP-fusion localizes to cytoplasm in punctate pattern
S000000732	YEL006W	YEA6	""	144327	145334	1	Mitochondrial NAD+ transporter	Mitochondrial NAD+ transporter; member of the mitochondrial carrier subfamily (see also YIA6); has putative human ortholog; YEA6 has a paralog, YIA6, that arose from the whole genome duplication; human NAD+ transporter MCART1/SLC25A51 functionally complements the yia6 yea6 double null mutant, and yeast YEA6 reciprocally complements defects in MCART1/SLC25A51 null cells 
S000000733	YEL007W	MIT1	Muc1 expressed Independent of TEC1	141892	143892	1	Transcriptional regulator of pseudohyphal growth	Transcriptional regulator of pseudohyphal growth; protein with sequence similarity to S. pombe gti1+ (gluconate transport inducer 1) and C. albicans Wor1
S000000734	YEL008W	""	""	140512	140892	1	Putative protein of unknown function	Putative protein of unknown function; conserved among S. cerevisiae strains; YEL008W is not an essential gene; predicted to be involved in metabolism
S000000735	YEL009C	GCN4	General Control Nonderepressible	138918	139763	-1	bZIP transcriptional activator of amino acid biosynthetic genes	bZIP transcriptional activator of amino acid biosynthetic genes;  activator responds to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels; contains four upstream open reading frames (uORFs) in 5' untranslated region which regulate translation
S000000736	YEL010W	""	""	136279	136629	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000000737	YEL011W	GLC3	GLyCogen	133120	135234	1	Glycogen branching enzyme, involved in glycogen accumulation	Glycogen branching enzyme, involved in glycogen accumulation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress; glycogen accumulation defect of the null mutant is functionally complemented by human GBE1, which is associated with glycogen storage disease
S000000738	YEL012W	UBC8	UBiquitin-Conjugating	131772	132551	1	Ubiquitin-conjugating enzyme that regulates gluconeogenesis	Ubiquitin-conjugating enzyme that regulates gluconeogenesis; negatively regulates gluconeogenesis by mediating the glucose-induced ubiquitination of fructose-1,6-bisphosphatase (FBPase); cytoplasmic enzyme that catalyzes the ubiquitination of histones in vitro
S000000739	YEL013W	VAC8	VACuole related	128825	130561	1	Vacuole-specific Myo2p receptor	Vacuole-specific Myo2p receptor; Myo2p-Vac17p-Vac8p transport complex subunit required for vacuolar inheritance; mediates anchoring of phosphatidylinositol 3-kinase complex I (PIK3C3-C1) to autophagosome; required with Atg13p for the vesicle closure step of the cytoplasm-to-vacuole (CVT) pathway, for homotypic vacuole-vacuole fusion and for nucleus-vacuole junction formation with Nvj1p; contains 11 armadillo (ARM) repeats; myristoylated, palmitoylated, and phosphorylated
S000000740	YEL014C	""	""	128303	128608	-1	Putative protein of unknown function	Putative protein of unknown function; conserved among S. cerevisiae strains
S000000741	YEL015W	EDC3	Enhancer of mRNA DeCapping	126629	128284	1	Non-essential conserved protein with a role in mRNA decapping	Non-essential conserved protein with a role in mRNA decapping; specifically affects the function of the decapping enzyme Dcp1p; mediates decay of the RPS28B mRNA via binding to both Rps28Bp (or Rps28Ap) and the RPS28B mRNA; mediates decay of the YRA1 mRNA by a different, translation-independent mechanism; localizes to cytoplasmic mRNA processing bodies; forms cytoplasmic foci upon DNA replication stress
S000000742	YEL016C	NPP2	ecto-Nucleotide Pyrophosphatase/Phosphodiesterase	124737	126218	-1	Nucleotide pyrophosphatase/phosphodiesterase	Nucleotide pyrophosphatase/phosphodiesterase; mediates extracellular nucleotide phosphate hydrolysis along with Npp1p and Pho5p; activity and expression enhanced during conditions of phosphate starvation; involved in spore wall assembly; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum; NPP2 has a paralog, NPP1, that arose from the whole genome duplication; npp1 npp2 double mutant exhibits reduced dityrosine fluorescence relative to single mutants
S000000743	YEL017W	GTT3	GlutaThione Transferase	123657	124670	1	Protein of unknown function may be involved in glutathione metabolism	Protein of unknown function may be involved in glutathione metabolism; function suggested by computational analysis of large-scale protein-protein interaction data; N- and C-terminal fusion proteins localize to the cell periphery
S000000746	YEL020C	PXP1	PeroXisomal Protein	118617	120299	-1	Peroxisomal matrix protein	Peroxisomal matrix protein; well-conserved in fungi; contains tripartite homology domain of thiamine pyrophosphate (TPP) enzymes; targeted to peroxisomes by Pex5p; contains low sequence identity with Pdc1p; mRNA identified as translated by ribosome profiling data
S000000748	YEL022W	GEA2	Guanine nucleotide Exchange on ARF	111421	115800	1	Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs)	Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication
S000000749	YEL023C	""	""	108504	110552	-1	Putative protein of unknown function	Putative protein of unknown function; expression is increased greatly during sporulation; YEL023C is not an essential gene
S000000750	YEL024W	RIP1	Rieske Iron-sulfur Protein	107260	107907	1	Ubiquinol-cytochrome-c reductase	Ubiquinol-cytochrome-c reductase; a Rieske iron-sulfur protein of the mitochondrial cytochrome bc1 complex; transfers electrons from ubiquinol to cytochrome c1 during respiration; during import, Rip1p is first imported into the mitochondrial matrix where it is processed, acquires its Fe-S cluster, and is folded, then is translocated into the inner membrane by the action of a homo-oligomer of Bcs1p, and finally is delivered by Bcs1p to Complex III for assembly
S000000751	YEL025C	""	""	102581	106147	-1	Putative protein of unknown function	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
S000000753	YEL027W	VMA3	Vacuolar Membrane Atpase	100769	101251	1	Proteolipid subunit c of the V0 domain of vacuolar H(+)-ATPase	Proteolipid subunit c of the V0 domain of vacuolar H(+)-ATPase; dicyclohexylcarbodiimide binding subunit; required for vacuolar acidification and important for copper and iron metal ion homeostasis
S000000754	YEL028W	""	""	98668	99129	1	Putative protein of unknown function	Putative protein of unknown function; conserved among S. cerevisiae strains; YEL028W is not an essential gene
S000000755	YEL029C	BUD16	BUD site selection	96858	97796	-1	Putative pyridoxal kinase	Putative pyridoxal kinase; a key enzyme involved in pyridoxal 5'-phosphate synthesis, the active form of vitamin B6; required for genome integrity; involved in bud-site selection; localizes to cytosol and peroxisomes; similarity to yeast BUD17 and human pyridoxal kinase (PDXK)
S000000756	YEL030W	ECM10	ExtraCellular Mutant	94644	96578	1	Heat shock protein of the Hsp70 family	Heat shock protein of the Hsp70 family; localized in mitochondrial nucleoids, plays a role in protein translocation, interacts with Mge1p in an ATP-dependent manner; overexpression induces extensive mitochondrial DNA aggregations; ECM10 has a paralog, SSC1, that arose from the whole genome duplication
S000000757	YEL031W	SPF1	Sensitivity to Pichia Farinosa killer toxin	90258	93905	1	P-type ATPase, ion transporter of the ER membrane	P-type ATPase, ion transporter of the ER membrane; required to maintain normal lipid and sterol composition of intracellular compartments and proper targeting of mitochondrial outer membrane tail-anchored proteins; involved in ER function and Ca2+ homeostasis; required for regulating Hmg2p degradation; confers sensitivity to a killer toxin (SMKT) produced by Pichia farinosa KK1; null mutation is complemented by human Parkinson disease-related ATP13A1 
S000000758	YEL032W	MCM3	MiniChromosome Maintenance	86937	89852	1	Protein involved in DNA replication	Protein involved in DNA replication; component of the Mcm2-7 hexameric helicase complex that binds chromatin as a part of the pre-replicative complex
S000000759	YEL033W	MTC7	Maintenance of Telomere Capping	86179	86598	1	Protein of unknown function	Protein of unknown function; predicted metabolic role based on network analysis derived from ChIP experiments, a large-scale deletion study and localization of transcription factor binding sites; null mutant is sensitive to temperature oscillation in a cdc13-1 mutant
S000000761	YEL035C	UTR5	Unidentified TRanscript	85045	85545	-1	Protein of unknown function	Protein of unknown function; originally considered essential but deletion overlapped the TATA box of neighboring essential gene HYP2, while deletion that avoids the HYP2 TATA box is viable; transcription may be regulated by Gcr1p
S000000762	YEL036C	ANP1	ANP and osmotic sensitive	83050	84552	-1	Subunit of the alpha-1,6 mannosyltransferase complex	Subunit of the alpha-1,6 mannosyltransferase complex; type II membrane protein; has a role in retention of glycosyltransferases in the Golgi; involved in osmotic sensitivity and resistance to aminonitrophenyl propanediol
S000000763	YEL037C	RAD23	RADiation sensitive	81407	82603	-1	Proteasome-associated ubiquitin receptor	Proteasome-associated ubiquitin receptor; K48-specific Ub chain binding protein that recruits substrates to the proteasome; subunit of nuclear excision repair factor 2 (NER2) with Rad4p that binds damaged DNA during NER, linking repair to proteolysis; NER2 binds DDR gene promoters to repress transcription in the absence of damage; stimulates Png1p-induced protein deglycosylation; contains a Ub-like (UBL) and two Ub-associated (UBA) domains; UBA2 protects Rad23p from proteasomal degradation
S000000764	YEL038W	UTR4	Unidentified TRanscript	80462	81145	1	Protein with sequence similarity to acireductone synthases	Protein with sequence similarity to acireductone synthases; involved in methionine salvage; found in both the cytoplasm and nucleus
S000000765	YEL039C	CYC7	CYtochrome C	79636	79977	-1	Cytochrome c isoform 2, expressed under hypoxic conditions	Cytochrome c isoform 2, expressed under hypoxic conditions; also known as iso-2-cytochrome c; electron carrier of the mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; protein abundance increases in response to DNA replication stress; CYC7 has a paralog, CYC1, that arose from the whole genome duplication
S000000766	YEL040W	UTR2	Unidentified TRanscript	78053	79456	1	Chitin transglycosylase	Chitin transglycosylase; functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar to and functionally redundant with Crh1; glycosylphosphatidylinositol (GPI)-anchored protein localized to bud neck
S000000767	YEL041W	YEF1	""	75944	77431	1	ATP-NADH kinase	ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; similar to Pos5p; overexpression complements certain pos5 phenotypes; YEF1 has a paralog, UTR1, that arose from the whole genome duplication
S000000768	YEL042W	GDA1	Guanosine DiphosphatAse	73771	75327	1	Guanosine diphosphatase located in the Golgi	Guanosine diphosphatase located in the Golgi; involved in the transport of GDP-mannose into the Golgi lumen, converting GDP to GMP after mannose is transferred to substrates; null mutants are defective in sporulation and pre-meiotic S phase entry; orthologous to human ENTPD6, a meiosis-associated non-obstructive azoospermia (NOA) related gene identified in GWAS studies
S000000769	YEL043W	GTA1	Golgi vesicle Trafficking Associated	70478	73348	1	Protein of unknown function	Protein of unknown function; proposed role in Golgi vesicle trafficking based on phenotypic profile, presence of a coiled-coil domain often associated with vesicle tethering proteins, and interaction profile process enrichment; may interact with ribosomes, based on co-purification studies; localizes to the ER and cell periphery in HTP studies; contains fibronectin type III domain fold 
S000000770	YEL044W	IES6	Ino Eighty Subunit	69757	70257	1	Component of the INO80 chromatin remodeling complex	Component of the INO80 chromatin remodeling complex; critical for INO80 function; involved in regulation of chromosome segregation and maintenance of normal centromeric chromatin structure; human ortholog INO80C is a member of the human INO80 complex; implicated in DNA repair based on genetic interactions with RAD52 epistasis genes
S000000771	YEL045C	""	""	68840	69265	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress
S000000772	YEL046C	GLY1	GLYcine requiring	67629	68792	-1	Threonine aldolase	Threonine aldolase; catalyzes the cleavage of L-allo-threonine and L-threonine to glycine; involved in glycine biosynthesis
S000000773	YEL047C	FRD1	Fumarate ReDuctase	65385	66797	-1	Soluble fumarate reductase	Soluble fumarate reductase; required with isoenzyme Osm1p for anaerobic growth; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; similar to Arxula adeninovorans fumarate reductase; protein abundance increases in response to DNA replication stress; FRD1 has a paralog, OSM1, that arose from the whole genome duplication
S000000774	YEL048C	TCA17	TRAPP Complex Associated protein	64709	65167	-1	Component of transport protein particle (TRAPP) complex II	Component of transport protein particle (TRAPP) complex II; TRAPPII is a multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating intra-Golgi and endosome-Golgi traffic; promotes association of TRAPPII-specific subunits with the TRAPP core complex; sedlin related; human Sedlin mutations cause SEDT, a skeletal disorder
S000000775	YEL049W	PAU2	seriPAUperin family	63728	64090	1	Member of the seripauperin multigene family	Member of the seripauperin multigene family; encoded mainly in subtelomeric region; active during alcoholic fermentation; regulated by anaerobiosis; negatively regulated by oxygen; repressed by heme 
S000000776	YEL050C	RML2	Ribosomal Mitochondrial Large	59670	60851	-1	Mitochondrial ribosomal protein of the large subunit (L2)	Mitochondrial ribosomal protein of the large subunit (L2); has similarity to E. coli L2 ribosomal protein; mutant allele (fat21) causes inability to utilize oleate, and induce oleic acid oxidation; may interfere with activity of the Adr1p transcription factor
S000000777	YEL051W	VMA8	Vacuolar Membrane Atpase	58378	59148	1	Subunit D of the V1 peripheral membrane domain of V-ATPase	Subunit D of the V1 peripheral membrane domain of V-ATPase; part of the electrogenic proton pump found throughout the endomembrane system; plays a role in the coupling of proton transport and ATP hydrolysis; the V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase) has eight subunits
S000000778	YEL052W	AFG1	ATPase Family Gene	56571	58100	1	Protein that may act as a chaperone for cytochrome c oxidase subunits	Protein that may act as a chaperone for cytochrome c oxidase subunits; conserved protein; may act as a chaperone in the degradation of misfolded or unassembled cytochrome c oxidase subunits; localized to matrix face of the mitochondrial inner membrane; member of the AAA family but lacks a protease domain
S000000779	YEL053C	MAK10	MAintenance of Killer	53901	56102	-1	Non-catalytic subunit of the NatC N-terminal acetyltransferase	Non-catalytic subunit of the NatC N-terminal acetyltransferase; required for replication of dsRNA virus; expression is glucose-repressible; human NatC ortholog, Naa35, requires co-expression of the human catalytic subunit, Naa30, to functionally complement the null allele
S000000780	YEL054C	RPL12A	Ribosomal Protein of the Large subunit	52721	53218	-1	Ribosomal 60S subunit protein L12A	Ribosomal 60S subunit protein L12A; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12A has a paralog, RPL12B, that arose from the whole genome duplication
S000000781	YEL055C	POL5	POLymerase	48471	51539	-1	Protein involved in 60S assembly and pre-40S AFs recycling	Protein involved in 60S assembly and pre-40S AFs recycling; has sequence similarity to human MybBP1A and weak sequence similarity to B-type DNA polymerases; not required for chromosomal DNA replication
S000000782	YEL056W	HAT2	Histone AcetylTransferase	47168	48373	1	Subunit of the Hat1p-Hat2p histone acetyltransferase complex	Subunit of the Hat1p-Hat2p histone acetyltransferase complex; required for high affinity binding of the complex to free histone H4, thereby enhancing Hat1p activity; similar to human RbAp46 and 48; has a role in telomeric silencing
S000000783	YEL057C	SDD1	Suppressor of Degenerative Death	45020	45721	-1	Protein of unknown function	Protein of unknown function; overproduction suppresses lethality due to expression of the dominant PET9 allele AAC2-A128P; may have a role in telomere maintenance; target of UME6 regulation
S000000784	YEL058W	PCM1	PhosphoaCetylglucosamine Mutase	43252	44925	1	Essential N-acetylglucosamine-phosphate mutase	Essential N-acetylglucosamine-phosphate mutase; converts GlcNAc-6-P to GlcNAc-1-P, which is a precursor for the biosynthesis of chitin and for the formation of N-glycosylated mannoproteins and glycosylphosphatidylinositol anchors
S000000785	YEL059W	HHY1	Hypersensitivity to HYgromycin B	42652	42960	1	Putative protein of unknown function	Putative protein of unknown function; null mutant is sensitive to heat, pH disturbances, ionic stress, and various chemicals; hypersensitivity to hygromycin B indicative of defects in vacuolar trafficking
S000000786	YEL060C	PRB1	PRoteinase B	40046	41953	-1	Vacuolar proteinase B yscB with H3 N-terminal endopeptidase activity	Vacuolar proteinase B yscB with H3 N-terminal endopeptidase activity; serine protease of subtilisin family; involved in protein degradation in vacuole; required for full protein degradation during sporulation; activity inhibited by Pbi2p; protein abundance increases in response to DNA replication stress; targeted to vacuole via Vps10p-dependent endosomal vacuolar protein sorting pathway and via AP-3 pathway; PRB1 has a paralog, YSP3, that arose from the whole genome duplication
S000000787	YEL061C	CIN8	Chromosome INstability	36535	39537	-1	Bipolar kinesin motor protein	Bipolar kinesin motor protein; switches from minus- to plus-end-directed motility in vitro depending on conditions; involved in mitotic spindle assembly and chromosome segregation
S000000788	YEL062W	NPR2	Nitrogen Permease Regulator	34407	36254	1	Subunit of the Iml1p/SEACIT complex	Subunit of the Iml1p/SEACIT complex; SEACIT (Iml1p-Npr2p-Npr3p) is a subcomplex of the SEA complex, a coatomer-related complex that associates dynamically with the vacuole; Npr2p may have a structural or regulatory role, supporting Iml1p function as a GAP for the Rag family GTPase Gtr1p, and resulting in inhibition of TORC1 signaling in response to amino acid deprivation; SEACIT is required for non-nitrogen-starvation-induced autophagy; homolog of human tumor suppressor NPRL2
S000000789	YEL063C	CAN1	CANavanine resistance	31694	33466	-1	Plasma membrane arginine permease	Plasma membrane arginine permease; requires phosphatidyl ethanolamine (PE) for localization, exclusively associated with lipid rafts; mutation confers canavanine resistance; CAN1 has a paralog, ALP1, that arose from the whole genome duplication
S000000790	YEL064C	AVT2	Amino acid Vacuolar Transport	29797	31239	-1	Putative transporter	Putative transporter; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
S000000791	YEL065W	SIT1	Siderophore Iron Transport	27657	29543	1	Ferrioxamine B transporter	Ferrioxamine B transporter; member of the ARN family of transporters that specifically recognize siderophore-iron chelates; transcription is induced during iron deprivation and diauxic shift; potentially phosphorylated by Cdc28p
S000000792	YEL066W	HPA3	Histone and other Protein Acetyltransferase	26721	27206	1	D-Amino acid N-acetyltransferase that detoxifies D-amino acids	D-Amino acid N-acetyltransferase that detoxifies D-amino acids; catalyzes N-acetylation of D-amino acids through ordered bi-bi mechanism in which acetyl-CoA is first substrate bound and CoA is last product liberated; acetylates histones and polyamines, also autoacetylates
S000000793	YEL067C	""	""	26189	26776	-1	Putative protein of unknown function	Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
S000000794	YEL068C	""	""	25646	25978	-1	Protein of unknown function	Protein of unknown function; expressed at both mRNA and protein levels; SWAT-GFP and seamless-GFP fusion proteins localize to the endoplasmic reticulum and mCherry fusion protein localizes to the vacuole
S000000797	YEL071W	DLD3	D-Lactate Dehydrogenase	16355	17845	1	2-hydroxyglutarate transhydrogenase, and minor D-lactate dehydrogenase	2-hydroxyglutarate transhydrogenase, and minor D-lactate dehydrogenase; converts D-2-hydroxyglutarate (D-2HG), an oncometabolite, to alpha-ketoglutarate in the presence of FAD, with concomitant reduction of pyruvate to D-lactate; minor lactate dehydrogenase activity; component of the retrograde regulon that consists of genes whose expression are stimulated by damage to mitochondria and reduced in cells grown with glutamate as the sole nitrogen source; located in the cytoplasm
S000000798	YEL072W	RMD6	Required for Meiotic nuclear Division	13720	14415	1	Protein required for sporulation	Protein required for sporulation; localizes to the peroxisome
S000000803	YER001W	MNN1	MaNNosyltransferase	153520	155808	1	Alpha-1,3-mannosyltransferase	Alpha-1,3-mannosyltransferase; integral membrane glycoprotein of Golgi complex, required for addition of alpha1,3-mannose linkages to N-linked and O-linked oligosaccharides; one of five S. cerevisiae proteins of MNN1 family; targeted to vacuole via AP-3 pathway
S000000804	YER002W	NOP16	NucleOlar Protein	156803	157498	1	Constituent of 66S pre-ribosomal particles	Constituent of 66S pre-ribosomal particles; involved in 60S ribosomal subunit biogenesis
S000000805	YER003C	PMI40	PhosphoMannose Isomerase	157736	159118	-1	Mannose-6-phosphate isomerase	Mannose-6-phosphate isomerase; catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation
S000000806	YER004W	FMP52	Found in Mitochondrial Proteome	159580	160275	1	Protein of unknown function	Protein of unknown function; localized to the mitochondrial outer membrane; induced by treatment with 8-methoxypsoralen and UVA irradiation
S000000807	YER005W	YND1	Yeast Nucleoside Diphosphatase	160550	162442	1	Apyrase with wide substrate specificity	Apyrase with wide substrate specificity; helps prevent inhibition of glycosylation by hydrolyzing nucleoside tri- and diphosphates that inhibit glycotransferases; partially redundant with Gda1p; mediates adenovirus E4orf4-induced toxicity
S000000808	YER006W	NUG1	NUclear GTPase	162723	164285	1	GTPase that associates with nuclear 60S pre-ribosomes	GTPase that associates with nuclear 60S pre-ribosomes; required for export of 60S ribosomal subunits from the nucleus
S000000809	YER007W	PAC2	Perish in the Absence of Cin8p	164527	166083	1	Microtubule effector required for tubulin heterodimer formation	Microtubule effector required for tubulin heterodimer formation; binds alpha-tubulin, required for normal microtubule function, null mutant exhibits cold-sensitive microtubules and sensitivity to benomyl
S000000810	YER008C	SEC3	SECretory	167808	171818	-1	Subunit of the exocyst complex	Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in a Rho1p-dependent, actin-independent manner, targeting and anchoring the exocyst to the plasma membrane with Exo70p; direct GTP Rho1p effector; required for ER inheritance; relocalizes away from bud neck upon DNA replication stress
S000000812	YER010C	""	""	172634	173338	-1	Bifunctional HMG aldolase/oxaloacetate decarboxylase	Bifunctional HMG aldolase/oxaloacetate decarboxylase; requires divalent metal ions for activity; competitively inhibited by oxalate; forms a ring-shaped homotrimer; similar to members of the prokaryotic RraA family of class II (divalent metal ion dependent) pyruvate aldolases from the meta cleavage pathways of protocatechuate and gallate
S000000813	YER011W	TIR1	TIp1-Related	175248	176012	1	Cell wall mannoprotein	Cell wall mannoprotein; expression is downregulated at acidic pH and induced by cold shock and anaerobiosis; abundance is increased in cells cultured without shaking; member of the Srp1p/Tip1p family of serine-alanine-rich proteins
S000000814	YER012W	PRE1	PRoteinase yscE	177835	178431	1	Beta 4 subunit of the 20S proteasome	Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle
S000000815	YER013W	PRP22	Pre-mRNA Processing	178841	182278	1	DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase	DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase; associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes; required for proofreading the exon ligation reaction
S000000816	YER014W	HEM14	HEMe biosynthesis	182600	184219	1	Protoporphyrinogen oxidase	Protoporphyrinogen oxidase; a mitochondrial enzyme that catalyzes the seventh step in the heme biosynthetic pathway, converting protoporphyrinogen IX to protoporphyrin IX; inhibited by diphenyl ether-type herbicides
S000000817	YER015W	FAA2	Fatty Acid Activation	184541	186775	1	Medium chain fatty acyl-CoA synthetase	Medium chain fatty acyl-CoA synthetase; activates imported fatty acids; accepts a wide range of fatty acid chain lengths with a preference for medium chains, C9:0-C13:0; localized to the peroxisome; comparative analysis suggests that a mitochondrially targeted form may result from translation starting at a non-canonical codon upstream of the annotated start codon
S000000818	YER016W	BIM1	BInding to Microtubules	188277	189311	1	Microtubule plus end-tracking protein	Microtubule plus end-tracking protein; forms a complex with Kar9p that makes up the cortical microtubule capture site and delays the exit from mitosis when the spindle is oriented abnormally; homolog of human end binding protein 1 (EB1)
S000000819	YER017C	AFG3	ATPase Family Gene	189503	191788	-1	Mitochondrial inner membrane m-AAA protease component	Mitochondrial inner membrane m-AAA protease component; mediates degradation of misfolded or unassembled proteins; also required for correct assembly of mitochondrial enzyme complexes; involved in cytoplasmic mRNA translation and aging; expression of human homolog AFG3L2 can complement yeast yta12 afg3 double mutant
S000000821	YER019W	ISC1	Inositol phosphoSphingolipid phospholipase C	192797	194230	1	Inositol phosphosphingolipid phospholipase C	Inositol phosphosphingolipid phospholipase C; hydrolyzes complex sphingolipids to produce ceramide; regulates the spindle assembly checkpoint upstream of PP2A-Cdc55; activates genes required for non-fermentable carbon source metabolism during the diauxic shift; mediates Na+ and Li+ halotolerance; activated by phosphatidylserine, cardiolipin and phosphatidylglycerol; mitochondrial outer membrane protein; ortholog of mammalian neutral sphingomyelinase type 2
S000000822	YER020W	GPA2	G Protein Alpha subunit	195168	196517	1	Nucleotide binding alpha subunit of the heterotrimeric G protein	Nucleotide binding alpha subunit of the heterotrimeric G protein; interacts with the receptor Gpr1p, has signaling role in response to nutrients; required for the recruitment of Ras-GTP at the plasma membrane and in the nucleus
S000000823	YER021W	RPN3	Regulatory Particle Non-ATPase	196948	198519	1	Essential non-ATPase regulatory subunit of the 26S proteasome lid	Essential non-ATPase regulatory subunit of the 26S proteasome lid; similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control
S000000824	YER022W	SRB4	Suppressor of RNA polymerase B	198812	200875	1	Subunit of the RNA polymerase II mediator complex	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for basal RNA polymerase II transcription; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination
S000000825	YER023W	PRO3	PROline requiring	201076	201936	1	Delta 1-pyrroline-5-carboxylate reductase	Delta 1-pyrroline-5-carboxylate reductase; catalyzes the last step in proline biosynthesis
S000000826	YER024W	YAT2	""	202192	204963	1	Carnitine acetyltransferase	Carnitine acetyltransferase; has similarity to Yat1p, which is a carnitine acetyltransferase associated with the mitochondrial outer membrane
S000000827	YER025W	GCD11	General Control Derepressed	205251	206834	1	Gamma subunit of the translation initiation factor eIF2	Gamma subunit of the translation initiation factor eIF2; involved in the identification of the start codon; binds GTP when forming the ternary complex with GTP and tRNAi-Met; mutations in human ortholog cause X-linked intellectual disability (XLID) syndrome
S000000828	YER026C	CHO1	CHOline requiring	207644	208474	-1	Phosphatidylserine synthase	Phosphatidylserine synthase; functions in phospholipid biosynthesis; transcriptionally repressed by myo-inositol and choline
S000000829	YER027C	GAL83	GALactose metabolism	208979	210232	-1	One of three alternate beta-subunits of the Snf1 kinase complex	One of three alternate beta-subunits of the Snf1 kinase complex; allows nuclear localization of the Snf1 kinase complex in the presence of a nonfermentable carbon source; necessary and sufficient for phosphorylation of the Mig2p transcription factor in response to alkaline stress; functionally redundant with SIP1 and SIP2 for the phosphorylation of Mig1p in response to glucose deprivation; contains a glycogen-binding domain
S000000830	YER028C	MIG3	Multicopy Inhibitor of Growth	210692	211876	-1	Transcriptional regulator	Transcriptional regulator; partially nonfunctional in S288C strains but has a major role in catabolite repression and ethanol response in some other strains; involved in response to toxic agents; phosphorylation by Snf1p or the Mec1p pathway inactivates Mig3p, allowing induction of damage response genes
S000000832	YER030W	CHZ1	Chaperone for Htz1/H2A-H2B dimer	213437	213898	1	Histone chaperone for Htz1p/H2A-H2B dimer	Histone chaperone for Htz1p/H2A-H2B dimer; required for the stabilization of the Chz1p-Htz1-H2B complex; has overlapping function with Nap1p; null mutant displays weak sensitivity to MMS and benomyl; contains a highly conserved CHZ motif; protein abundance increases in response to DNA replication stress
S000000833	YER031C	YPT31	Yeast Protein Two	214076	214747	-1	Rab family GTPase	Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication; localizes to the transitional and late Golgi
S000000834	YER032W	FIR1	Factor Interacting with REF2	215063	217693	1	Protein involved in 3' mRNA processing	Protein involved in 3' mRNA processing; interacts with Ref2p; APCC(Cdh1) substrate; potential Cdc28p substrate
S000000835	YER033C	ZRG8	Zinc Regulated Gene	218057	221287	-1	Protein of unknown function	Protein of unknown function; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; GFP-fusion protein is localized to the cytoplasm; transcription induced under conditions of zinc deficiency
S000000836	YER034W	""	""	221846	222403	1	Protein of unknown function	Protein of unknown function; non-essential gene; expression induced upon calcium shortage; protein abundance increases in response to DNA replication stress
S000000837	YER035W	EDC2	Enhancer of mRNA DeCapping	222639	223076	1	RNA-binding protein that directly activates mRNA decapping	RNA-binding protein that directly activates mRNA decapping; binds mRNA substrate and enhances activity of decapping proteins Dcp1p and Dcp2p; has a role in translation during heat stress; protein increases in abundance and relocalizes to nucleolus and to nuclear foci upon DNA replication stress; EDC2 has a paralog, EDC1, that arose from the whole genome duplication
S000000838	YER036C	ARB1	ATP-binding cassette protein involved in Ribosome Biogenesis	223367	225199	-1	ATPase of the ATP-binding cassette (ABC) family	ATPase of the ATP-binding cassette (ABC) family; involved in 40S and 60S ribosome biogenesis, has similarity to Gcn20p; important for disaggregation of ordered and disordered aggregates; shuttles from nucleus to cytoplasm, physically interacts with Tif6p, Lsg1p; human homolog ABCF2 can complement yeast ARB1 mutant
S000000839	YER037W	PHM8	PHosphate Metabolism	225889	226854	1	Lysophosphatidic acid (LPA) phosphatase, nucleotidase	Lysophosphatidic acid (LPA) phosphatase, nucleotidase; principle and physiological nucleotidase working on GMP, UMP and CMP; involved in LPA hydrolysis in response to phosphate starvation and ribose salvage pathway; phosphatase activity is soluble and Mg2+ dependent; expression is induced by low phosphate levels and by inactivation of Pho85p; repressed by Gcn4p under normal conditions; PHM8 has a paralog, SDT1, that arose from the whole genome duplication
S000000840	YER038C	KRE29	Killer toxin REsistant	226858	228252	-1	Subunit of the SMC5-SMC6 complex	Subunit of the SMC5-SMC6 complex; this complex is involved in removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance
S000000841	YER039C	HVG1	Homologous to VRG4	228456	229205	-1	Protein of unknown function	Protein of unknown function; HVG1 has a paralog, VRG4, that arose from the whole genome duplication
S000000842	YER040W	GLN3	GLutamiNe metabolism	229795	231987	1	Transcriptional activator in nitrogen catabolite repression system	Transcriptional activator in nitrogen catabolite repression system; localization and activity regulated by quality of nitrogen source and Ure2p; full-length protein forms prion-like aggregates when overproduced
S000000843	YER041W	YEN1	""	232461	234740	1	Holliday junction resolvase	Holliday junction resolvase; promotes template switching during break-induced replication (BIR), causing non-reciprocal translocations (NRTs); localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1; similar to S. cerevisiae endonuclease Rth1p
S000000844	YER042W	MXR1	peptide Methionine sulfoXide Reductase	234937	235491	1	Methionine-S-sulfoxide reductase	Methionine-S-sulfoxide reductase; involved in the response to oxidative stress; protects iron-sulfur clusters from oxidative inactivation along with MXR2; involved in the regulation of lifespan; reduced activity of human homolog implicated in Alzheimer disease
S000000845	YER043C	SAH1	S-Adenosyl-l-Homocysteine hydrolase	235770	237119	-1	S-adenosyl-L-homocysteine hydrolase	S-adenosyl-L-homocysteine hydrolase; catabolizes S-adenosyl-L-homocysteine which is formed after donation of the activated methyl group of S-adenosyl-L-methionine (AdoMet) to an acceptor; regulates cellular lipid homoeostasis by regulating phosphatidylcholine(PC)synthesis and triacylglycerol (TG) levels
S000000846	YER044C	ERG28	ERGosterol biosynthesis	237570	238016	-1	Endoplasmic reticulum membrane protein	Endoplasmic reticulum membrane protein; may facilitate protein-protein interactions between the Erg26p dehydrogenase and the Erg27p 3-ketoreductase and/or tether these enzymes to the ER, also interacts with Erg6p
S000000847	YER045C	ACA1	ATF/CREB Activator	240032	241501	-1	ATF/CREB family basic leucine zipper (bZIP) transcription factor	ATF/CREB family basic leucine zipper (bZIP) transcription factor; binds as a homodimer to the ATF/CREB consensus sequence TGACGTCA; important for carbon source utilization; target genes include GRE2 and COS8; ACA1 has a paralog, CST6, that arose from the whole genome duplication
S000000848	YER046W	SPO73	SPOrulation	243180	243611	1	Meiosis-specific protein required for prospore membrane morphogenesis	Meiosis-specific protein required for prospore membrane morphogenesis; required for the proper shape of the prospore membrane (PSM) and for spore wall formation; functions cooperatively with SPO71 in PSM elongation; physically interacts with Spo71p; genetically antagonistic to SPO1, similar to SPO71; localizes to the PSM; required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis; dysferlin domain-only protein
S000000849	YER047C	SAP1	Sin1 Associated Protein	243810	246503	-1	Putative ATPase of the AAA family	Putative ATPase of the AAA family; interacts with the Sin1p transcriptional repressor in the two-hybrid system
S000000850	YER048C	CAJ1	""	246982	248157	-1	Nuclear type II J heat shock protein of the E. coli dnaJ family	Nuclear type II J heat shock protein of the E. coli dnaJ family; overexpression stabilizes amino acid permeases; contains a leucine zipper-like motif, binds to non-native substrates for presentation to Ssa3p, may function during protein translocation, assembly and disassembly; localizes to the cytosol and plasma membrane, while a GFP fusion protein localizes to the nucleus
S000000851	YER049W	TPA1	Termination and PolyAdenylation	251728	253662	1	Prolyl hydroxylase	Prolyl hydroxylase; catalyzes oxygen-dependent dihydroxylation of Rps23a/b, a 40S ribosomal decoding center subunit; influences translational termination and regulates translational accuracy; interacts with Sup45p (eRF1), Sup35p (eRF3) and Pab1p; disputed role as a oxidative dealkylase repairing DNA methyl-base lesions; poly(rA)-binding protein affecting poly(A) tail length and mRNA stability; Fe(II)/2-oxoglutarate-dependent dioxygenase family member similar to human prolyl 4-hydroxylase OGFOD1
S000000852	YER050C	RSM18	Ribosomal Small subunit of Mitochondria	253971	254387	-1	Mitochondrial ribosomal protein of the small subunit	Mitochondrial ribosomal protein of the small subunit; has similarity to E. coli S18 ribosomal protein
S000000853	YER051W	JHD1	JmjC domain-containing Histone Demethylase	254656	256134	1	JmjC domain family histone demethylase specific for H3-K36	JmjC domain family histone demethylase specific for H3-K36; similar to proteins found in human, mouse, drosophila, X. laevis, C. elegans, and S. pombe
S000000854	YER052C	HOM3	HOMoserine requiring	256375	257958	-1	Aspartate kinase (L-aspartate 4-P-transferase)	Aspartate kinase (L-aspartate 4-P-transferase); cytoplasmic enzyme that catalyzes the first step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis
S000000855	YER053C	PIC2	PI Carrier	258737	259639	-1	Mitochondrial copper and phosphate carrier	Mitochondrial copper and phosphate carrier; imports copper and inorganic phosphate into mitochondria; functionally redundant with Mir1p but less abundant than Mir1p under normal conditions; expression is induced at high temperature
S000000856	YER054C	GIP2	Glc7-Interacting Protein	262052	263698	-1	Putative regulatory subunit of protein phosphatase Glc7p	Putative regulatory subunit of protein phosphatase Glc7p; involved in glycogen metabolism; contains a conserved motif (GVNK motif) that is also found in Gac1p, Pig1p, and Pig2p; GIP2 has a paralog, PIG2, that arose from the whole genome duplication
S000000857	YER055C	HIS1	HIStidine	264892	265785	-1	ATP phosphoribosyltransferase	ATP phosphoribosyltransferase; a hexameric enzyme, catalyzes the first step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control
S000000858	YER056C	FCY2	FluoroCYtosine resistance	266512	268113	-1	Purine-cytosine permease	Purine-cytosine permease; mediates purine (adenine, guanine, and hypoxanthine) and cytosine accumulation; relative distribution to the vacuole increases upon DNA replication stress
S000000859	YER057C	HMF1	Homologous Mmf1p Factor	270737	271126	-1	Member of the p14.5 protein family	Member of the p14.5 protein family; functionally complements Mmf1p function when targeted to mitochondria; heat shock inducible; high-dosage growth inhibitor; forms a homotrimer in vitro; HMF1 has a paralog, MMF1, that arose from the whole genome duplication
S000000860	YER058W	PET117	PETite colonies	271768	272091	1	Assembly factor that couples heme a synthesis to complex IV assembly	Assembly factor that couples heme a synthesis to complex IV assembly; regulates the oligomerization state of the Cox15p heme a synthase, and couples this to the assembly of the cytochrome C oxidase complex (complex IV) 
S000000861	YER059W	PCL6	Pho85 CycLin	272624	273886	1	Pho85p cyclin of the Pho80p subfamily	Pho85p cyclin of the Pho80p subfamily; forms the major Glc8p kinase together with Pcl7p and Pho85p; involved in the control of glycogen storage by Pho85p; stabilized by Elongin C binding; PCL6 has a paralog, PCL7, that arose from the whole genome duplication
S000000862	YER060W	FCY21	FluoroCYtosine resistance	274567	276153	1	Putative purine-cytosine permease	Putative purine-cytosine permease; very similar to Fcy2p but cannot substitute for its function
S000000863	YER061C	CEM1	Condensing Enzyme with Mitochondrial function	278298	279626	-1	Mitochondrial beta-keto-acyl synthase	Mitochondrial beta-keto-acyl synthase; possible role in fatty acid synthesis; required for mitochondrial respiration; human homolog OXSM can complement yeast cem1 null mutant
S000000864	YER062C	GPP2	Glycerol-3-Phosphate Phosphatase	279930	280682	-1	DL-glycerol-3-phosphate phosphatase involved in glycerol biosynthesis	DL-glycerol-3-phosphate phosphatase involved in glycerol biosynthesis; also known as glycerol-1-phosphatase; induced in response to hyperosmotic or oxidative stress, and during diauxic shift; GPP2 has a paralog, GPP1, that arose from the whole genome duplication
S000000865	YER063W	THO1	suppressor of the Transcriptional defect of Hpr1 by Overexpression	281710	282366	1	Conserved nuclear RNA-binding protein	Conserved nuclear RNA-binding protein; specifically binds to transcribed chromatin in a THO- and RNA-dependent manner, genetically interacts with shuttling hnRNP NAB2; overproduction suppresses transcriptional defect caused by hpr1 mutation
S000000866	YER064C	VHR2	VHt1 Regulator	282705	284222	-1	Non-essential nuclear protein	Non-essential nuclear protein; null mutation has global effects on transcription; VHR2 has a paralog, VHR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
S000000867	YER065C	ICL1	IsoCitrate Lyase	285241	286914	-1	Isocitrate lyase	Isocitrate lyase; catalyzes the formation of succinate and glyoxylate from isocitrate, a key reaction of the glyoxylate cycle; expression of ICL1 is induced by growth on ethanol and repressed by growth on glucose
S000000868	YER066W	RRT13	Regulator of rDNA Transcription	290242	290799	1	Putative protein of unknown function	Putative protein of unknown function; non-essential gene identified in a screen for mutants with decreased levels of rDNA transcription
S000000869	YER067W	RGI1	Respiratory Growth Induced	292066	292551	1	Protein involved in energy metabolism under respiratory conditions	Protein involved in energy metabolism under respiratory conditions; abundance is increased upon intracellular iron depletion or in response to DNA replication stress; suppresses pro-apoptotic effect of expressing human BAX in yeast cells; RGI1 has a paralog, RGI2, that arose from the whole genome duplication
S000000870	YER068W	MOT2	Modulator Of Transcription	293050	294813	1	Ubiquitin-protein ligase subunit of the CCR4-NOT complex	Ubiquitin-protein ligase subunit of the CCR4-NOT complex; with Ubc4p, ubiquitinates nascent polypeptide-associated complex subunits and histone demethyase Jhd2p; CCR4-NOT has roles in transcription regulation, mRNA degradation, and post-transcriptional modifications; regulates levels of DNA Polymerase-{alpha} to promote efficient and accurate DNA replication
S000000871	YER069W	ARG56	ARGinine requiring	295410	298001	1	Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase	Acetylglutamate kinase and N-acetyl-gamma-glutamyl-phosphate reductase; N-acetyl-L-glutamate kinase (NAGK) catalyzes the 2nd and N-acetyl-gamma-glutamyl-phosphate reductase (NAGSA), the 3rd step in arginine biosynthesis; synthesized as a precursor which is processed in the mitochondrion to yield mature NAGK and NAGSA; enzymes form a metabolon complex with Arg2p; NAGK C-terminal domain stabilizes the enzymes, slows catalysis and is involved in feed-back inhibition by arginine
S000000872	YER070W	RNR1	RiboNucleotide Reductase	298950	301616	1	Major isoform of large subunit of ribonucleotide-diphosphate reductase	Major isoform of large subunit of ribonucleotide-diphosphate reductase; the RNR complex catalyzes rate-limiting step in dNTP synthesis, regulated by DNA replication and DNA damage checkpoint pathways via localization of small subunits; relative distribution to the nucleus increases upon DNA replication stress; RNR1 has a paralog, RNR3, that arose from the whole genome duplication
S000000873	YER071C	TDA2	Topoisomerase I Damage Affected	301947	302327	-1	Subunit of a complex that associates with actin filaments	Subunit of a complex that associates with actin filaments; forms a complex with Aim21p that inhibits barbed end F-actin assembly; elevates actin monomer pools to increase endocytotic efficiency and to regulate the distribution of actin between cables and patches; Aim21p/Tda2p forms a larger complex with actin capping proteins Cap1p and Cap2p; TcTex1-type dynein light chain family member; null mutant is sensitive to expression of the top1-T722A allele
S000000874	YER072W	VTC1	Vacuolar Transporter Chaperone	302806	303195	1	Regulatory subunit of vacuolar transporter chaperone (VTC) complex	Regulatory subunit of vacuolar transporter chaperone (VTC) complex; VTC complex is involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion; important regulator of substrate invagination from the vacuolar membrane; also binds mRNA; targeted to vacuole via AP-3 pathway; protein abundance increases in response to DNA replication stress
S000000875	YER073W	ALD5	ALdehyde Dehydrogenase	304030	305592	1	Mitochondrial aldehyde dehydrogenase	Mitochondrial aldehyde dehydrogenase; involved in regulation or biosynthesis of electron transport chain components and acetate formation; activated by K+; utilizes NADP+ as the preferred coenzyme; constitutively expressed
S000000876	YER074W	RPS24A	Ribosomal Protein of the Small subunit	306323	307196	1	Protein component of the small (40S) ribosomal subunit	Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24A has a paralog, RPS24B, that arose from the whole genome duplication
S000000877	YER075C	PTP3	Protein Tyrosine Phosphatase	308413	311199	-1	Phosphotyrosine-specific protein phosphatase	Phosphotyrosine-specific protein phosphatase; major role in the pheromone adaptive response through dephosphorylation of the Fus3p MAPK with a minor role by Ptp2p and Msg5p; minor role in the inactivation of Hog1p MAPK during osmolarity sensing with major role by Ptp2p; dephosphorylates and regulates the localization of Hog1p; minor role in the cell wall integrity pathway through tyrosine dephosphorylation of the MAPK Slt2p while Ptp2p plays a major role; localizes to the cytoplasm
S000000878	YER076C	""	""	312590	313498	-1	Putative protein of unknown function	Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; analysis of HA-tagged protein suggests a membrane localization
S000000879	YER077C	MRX1	Mitochondrial oRganization of gene eXpression (MIOREX)	314534	316600	-1	Protein that associates with mitochondrial ribosome	Protein that associates with mitochondrial ribosome; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport
S000000880	YER078C	ICP55	Intermediate Cleaving Peptidase	316807	318342	-1	Mitochondrial aminopeptidase	Mitochondrial aminopeptidase; cleaves N termini of at least 38 imported proteins after cleavage by the mitochondrial processing peptidase (MPP), thereby increasing their stability; member of aminopeptidase P family; exists in rapid equilibrium between monomer and dimer, with dimer being active species; ortholog of human gene XPNPEP3, which is mutated in rare hereditary kidney disease similar to nephronophthisis
S000000881	YER079W	""	""	318920	319552	1	Putative protein of unknown function	Putative protein of unknown function
S000000882	YER080W	AIM9	Altered Inheritance rate of Mitochondria	319963	321846	1	Protein of unknown function	Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays elevated frequency of mitochondrial genome loss
S000000883	YER081W	SER3	SERine requiring	322686	324095	1	3-phosphoglycerate dehydrogenase and alpha-ketoglutarate reductase	3-phosphoglycerate dehydrogenase and alpha-ketoglutarate reductase; 3PG dehydrogenase that catalyzes the first step in serine and glycine biosynthesis; also functions as an alpha-ketoglutarate reductase, converting alpha-ketoglutarate to D-2-hydroxyglutarate (D-2HG); localizes to the cytoplasm; SER3 has a paralog, SER33, that arose from the whole genome duplication
S000000885	YER083C	GET2	Guided Entry of Tail-anchored proteins	326174	327031	-1	Subunit of the GET complex	Subunit of the GET complex; involved in insertion of  tail-anchored proteins proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division; Get1p and Get2p form an aqueous channel for protein translocation and insertion into the membrane
S000000886	YER084W	""	""	327065	327451	1	Protein of unknown function	Protein of unknown function; expressed at both mRNA and protein levels
S000000887	YER085C	""	""	327619	328140	-1	Putative protein of unknown function	Putative protein of unknown function
S000000888	YER086W	ILV1	IsoLeucine-plus-Valine requiring	328477	330207	1	Threonine deaminase, catalyzes first step in isoleucine biosynthesis	Threonine deaminase, catalyzes first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation
S000000889	YER087W	AIM10	Altered Inheritance rate of Mitochondria	330576	332306	1	Protein with similarity to tRNA synthetases	Protein with similarity to tRNA synthetases; non-tagged protein is detected in purified mitochondria; null mutant is viable and displays elevated frequency of mitochondrial genome loss
S000000890	YER088C	DOT6	Disruptor Of Telomeric silencing	333176	335188	-1	Protein involved in rRNA and ribosome biogenesis	Protein involved in rRNA and ribosome biogenesis; activated in stochastic pulses of nuclear localization; binds polymerase A and C motif; subunit of the RPD3L histone deacetylase complex; has chromatin specific SANT domain; involved in telomeric gene silencing and filamentation; relative distribution to the nucleus increases upon DNA replication stress
S000000891	YER089C	PTC2	Phosphatase Two C	335946	337340	-1	Type 2C protein phosphatase (PP2C)	Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p to limit maximal osmostress induced kinase activity; dephosphorylates Ire1p to downregulate the unfolded protein response; dephosphorylates Cdc28p; inactivates the DNA damage checkpoint; PTC2 has a paralog, PTC3, that arose from the whole genome duplication
S000000892	YER090W	TRP2	TRyPtophan	337949	339472	1	Anthranilate synthase	Anthranilate synthase; catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
S000000893	YER091C	MET6	METhionine requiring	339864	342167	-1	Cobalamin-independent methionine synthase	Cobalamin-independent methionine synthase; involved in methionine biosynthesis and regeneration; requires a minimum of two glutamates on the methyltetrahydrofolate substrate, similar to bacterial metE homologs
S000000894	YER092W	IES5	Ino Eighty Subunit	342855	343232	1	Non-essential INO80 chromatin remodeling complex subunit	Non-essential INO80 chromatin remodeling complex subunit; deletion affects telomere maintenance via recombination
S000000897	YER095W	RAD51	RADiation sensitive	349980	351182	1	Strand exchange protein	Strand exchange protein; forms helical filament with DNA that searches for homology; involved in recombinational repair of DNA DSBs during vegetative growth and meiosis; phosphorylation by Cdc28p in G2/M phase promotes DNA binding, strand invasion, and primer extension; L1 and L2 motifs of DNA binding site I have critical roles in ensuring DNA fidelity during meiotic recombination; RAD51 and RAD4 pathways confer resistance to benzo[a]pyrene dihydrodiol; homolog of Dmc1p and bacterial RecA
S000000898	YER096W	SHC1	Sporulation-specific Homolog of CSD4	351698	353236	1	Sporulation-specific activator of Chs3p (chitin synthase III)	Sporulation-specific activator of Chs3p (chitin synthase III); required for the synthesis of the chitosan layer of ascospores; transcriptionally induced at alkaline pH; SHC1 has a paralog, SKT5, that arose from the whole genome duplication
S000000899	YER097W	""	""	355140	355469	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000000900	YER098W	UBP9	UBiquitin-specific Protease	355466	357730	1	Putative ubiquitin-specific protease that cleaves ubiquitin-protein fu	Putative ubiquitin-specific protease that cleaves ubiquitin-protein fusions; UBP9 has a paralog, UBP13, that arose from the whole genome duplication
S000000901	YER099C	PRS2	PhosphoRibosylpyrophosphate Synthetase	358105	359061	-1	5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase	5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase; synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; PRS2 has a paralog, PRS4, that arose from the whole genome duplication
S000000903	YER101C	AST2	ATPase STabilizing	360502	361794	-1	Lipid raft associated protein	Lipid raft associated protein; overexpression restores Pma1p localization to lipid rafts which is required for targeting of Pma1p to the plasma membrane; sometimes classified in the medium-chain dehydrogenase/reductases (MDRs) superfamily; AST2 has a paralog, AST1, that arose from the whole genome duplication
S000000905	YER103W	SSA4	Stress-Seventy subfamily A	364589	366517	1	Heat shock protein that is highly induced upon stress	Heat shock protein that is highly induced upon stress; plays a role in SRP-dependent cotranslational protein-membrane targeting and translocation; member of the HSP70 family; cytoplasmic protein that concentrates in nuclei upon starvation; SSA4 has a paralog, SSA3, that arose from the whole genome duplication
S000000907	YER105C	NUP157	NUclear Pore	367838	372013	-1	Subunit of the inner ring of the nuclear pore complex (NPC)	Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and tethering of DNA to the nuclear periphery; both Nup170p and NUP157p are similar to human Nup155p; NUP157 has a paralog, NUP170, that arose from the whole genome duplication
S000000908	YER106W	MAM1	Monopolar microtubule Attachment during Meiosis I	372326	373234	1	Monopolin	Monopolin; meiosis-specific kinetochore-associated protein involved in monopolar attachment of sister kinetochores to the meiotic spindle; subunit of monopolin, a complex that prevents biorientation of sister kinetochores to ensure homolog biorientation during meiosis I; regulates the conformation, enzyme kinetics and substrate specificity of the Dsn1p kinase, Hrr1p; expressed only during the first meiotic division
S000000911	YER109C	FLO8	FLOcculation	375215	377614	-1	Transcription factor	Transcription factor; required for flocculation, diploid filamentous growth, and haploid invasive growth; forms a heterodimer with Mss11p that interacts with the Swi/Snf complex during transcriptional activation of FLO1, FLO11, and STA1; S. cerevisiae genome reference strain S288C contains an internal in-frame stop at codon 142, which in other strains encodes tryptophan
S000000912	YER110C	KAP123	KAryoPherin	378762	382103	-1	Karyopherin beta	Karyopherin beta; mediates nuclear import of ribosomal proteins prior to assembly into ribosomes and import of histones H3 and H4; localizes to the nuclear pore, nucleus, and cytoplasm; exhibits genetic interactions with RAI1
S000000913	YER111C	SWI4	SWItching deficient	382595	385876	-1	DNA binding component of the SBF complex (Swi4p-Swi6p)	DNA binding component of the SBF complex (Swi4p-Swi6p); a transcriptional activator that in concert with MBF (Mbp1-Swi6p) regulates late G1-specific transcription of targets including cyclins and genes required for DNA synthesis and repair; Slt2p-independent regulator of cold growth; acetylation at two sites, K1016 and K1066, regulates interaction with Swi6p
S000000914	YER112W	LSM4	Like SM	387232	387795	1	Lsm (Like Sm) protein	Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; forms cytoplasmic foci upon DNA replication stress
S000000915	YER113C	TMN3	TransMembrane Nine	387932	390052	-1	Protein with a role in cellular adhesion and filamentous growth	Protein with a role in cellular adhesion and filamentous growth; similar to Emp70p and Tmn2p; member of Transmembrane Nine family with 9 transmembrane segments; localizes to Golgi; induced by 8-methoxypsoralen plus UVA irradiation
S000000916	YER114C	BOI2	Bem1 (One) Interacting protein	390590	393712	-1	Protein involved in polar growth	Protein involved in polar growth; functionally redundant with Boi1p; required for fusion of secretory vesicles with plasma membrane at sites of polarized growth and for inhibition of membrane abscission until the end of cytokinesis; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI2 has a paralog, BOI1, that arose from the whole genome duplication
S000000917	YER115C	SPR6	SPorulation Regulated	394292	394867	-1	Protein of unknown function	Protein of unknown function; expressed during sporulation; not required for sporulation, but gene exhibits genetic interactions with other genes required for sporulation
S000000918	YER116C	SLX8	Synthetic Lethal of unknown (X) function	395348	396172	-1	Subunit of Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex	Subunit of Slx5-Slx8 SUMO-targeted ubiquitin ligase (STUbL) complex; role in proteolysis of spindle positioning protein Kar9, DNA repair proteins Rad52p and Rad57p; stimulated by SUMO-modified substrates; contains a C-terminal RING domain; forms nuclear foci upon DNA replication stress; required for maintenance of genome integrity like human ortholog RNF4
S000000919	YER117W	RPL23B	Ribosomal Protein of the Large subunit	396769	397653	1	Ribosomal 60S subunit protein L23B	Ribosomal 60S subunit protein L23B; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23B has a paralog, RPL23A, that arose from the whole genome duplication
S000000920	YER118C	SHO1	Synthetic, High Osmolarity-sensitive	397952	399055	-1	Transmembrane osmosensor for filamentous growth and HOG pathways	Transmembrane osmosensor for filamentous growth and HOG pathways; involved in activation of the Cdc42p- and MAP kinase-dependent filamentous growth pathway and the high-osmolarity glycerol (HOG) response pathway; phosphorylated by Hog1p; interacts with Pbs2p, Msb2p, Hkr1p, and Ste11p
S000000921	YER119C	AVT6	Amino acid Vacuolar Transport	399496	400842	-1	Vacuolar aspartate and glutamate exporter	Vacuolar aspartate and glutamate exporter; member of a family of seven genes (AVT1-7) related to vesicular GABA-glycine transporters; involved in compartmentalizing acidic amino acids in response to nitrogen starvation; AVT6 has a paralog, AVT5, that arose from the whole genome duplication
S000000922	YER120W	SCS2	Suppressor of Choline Sensitivity	401135	401869	1	Integral ER membrane protein, regulates phospholipid metabolism	Integral ER membrane protein, regulates phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to plasma membrane (PM) and regulate PI4P levels by controlling access of Sac1p phosphatase to substrate PI4P in the PM; interacts with FFAT motifs in Opi1p, Swh1p, Osh2p, and Osh3p; involved in telomeric silencing; VAP homolog; SCS2 has a paralog, SCS22, that arose from the whole genome duplication
S000000923	YER121W	""	""	402375	402719	1	Putative protein of unknown function	Putative protein of unknown function; may be involved in phosphatase regulation and/or generation of precursor metabolites and energy
S000000924	YER122C	GLO3	GLyOxalase	402871	404352	-1	ADP-ribosylation factor GTPase activating protein (ARF GAP)	ADP-ribosylation factor GTPase activating protein (ARF GAP); involved in ER-Golgi transport; shares functional similarity with Gcs1p
S000000925	YER123W	YCK3	Yeast Casein Kinase	404813	406387	1	Palmitoylated vacuolar membrane-localized casein kinase I isoform	Palmitoylated vacuolar membrane-localized casein kinase I isoform; negatively regulates vacuole fusion during hypertonic stress via phosphorylation of Vps41p; shares essential functions with Hrr25p; targeted to vacuole via AP-3 pathway; regulates vesicle fusion in AP-3 pathway
S000000926	YER124C	DSE1	Daughter Specific Expression	407342	409063	-1	Daughter cell-specific protein	Daughter cell-specific protein; may regulate cross-talk between the mating and filamentation pathways; deletion affects cell separation after division and sensitivity to alpha-factor and drugs affecting the cell wall; relocalizes from bud neck to cytoplasm upon DNA replication stress
S000000927	YER125W	RSP5	Reverses Spt- Phenotype	410189	412618	1	NEDD4 family E3 ubiquitin ligase	NEDD4 family E3 ubiquitin ligase; regulates processes including: MVB sorting, the heat shock response, transcription, endocytosis and ribosome stability; ubiquitinates Sec23p, Sna3p, Ste4p, Nfi1p, Rpo21p, Sem1p, Dip5p and Put4p; autoubiquitinates; deubiquitinated by Ubp2p; regulated by SUMO ligase Siz1p, in turn regulates Siz1p SUMO ligase activity; required for efficient Golgi-to-ER trafficking in COPI mutants; mutant tolerates aneuploidy; human homolog implicated in Liddle syndrome
S000000928	YER126C	NSA2	Nop Seven Associated	413394	414179	-1	Protein constituent of 66S pre-ribosomal particles	Protein constituent of 66S pre-ribosomal particles; contributes to processing of the 27S pre-rRNA; recruited by ribosomal proteins L17, L35, and L37 to assembling ribosomes after 27SB pre-rRNA is generated, immediately preceding removal of ITS2
S000000930	YER128W	VFA1	Vps Four-Associated	415859	416470	1	Protein that interacts with Vps4p and has a role in vacuolar sorting	Protein that interacts with Vps4p and has a role in vacuolar sorting; stimulates the ATPase activity of Vps4; localizes to endosomes in a Vps4-dependent manner; overexpression causes canavanine sensitivity and confers a partial class D vacuole morphology
S000000931	YER129W	SAK1	Snf1 Activating Kinase	417281	420709	1	Upstream serine/threonine kinase for the SNF1 complex	Upstream serine/threonine kinase for the SNF1 complex; plays a role in pseudohyphal growth; partially redundant with Elm1p and Tos3p; members of this family have functional orthology with LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome; SAK1 has a paralog, TOS3, that arose from the whole genome duplication
S000000932	YER130C	COM2	Cousin of Msn2	421115	422446	-1	Transcription factor that binds IME1 Upstream Activation Signal (UAS)	Transcription factor that binds IME1 Upstream Activation Signal (UAS)ru; COM2 transcription is regulated by Haa1p, Sok2p and Zap1p transcriptional activators; may bind the IME1 promoter under all growth conditions to negatively regulate its transcription in the absence of a positive regulator that binds more effectively; repressor activity may depend on phosphorylation by PKA; C. albicans homolog (MNL1) plays a role in adaptation to stress
S000000933	YER131W	RPS26B	Ribosomal Protein of the Small subunit	423952	424311	1	Protein component of the small (40S) ribosomal subunit	Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S26, no bacterial homolog; RPS26B has a paralog, RPS26A, that arose from the whole genome duplication; human homolog can partially complement an RPS26A, RPS26B double null mutant; mutations in the human gene are associated with Diamond-Blackfan anemia
S000000934	YER132C	PMD1	Paralog of MDS3	425188	430449	-1	Protein with an N-terminal kelch-like domain	Protein with an N-terminal kelch-like domain; putative negative regulator of early meiotic gene expression; required, with Mds3p, for growth under alkaline conditions; PMD1 has a paralog, MDS3, that arose from the whole genome duplication
S000000935	YER133W	GLC7	GLyCogen	432495	433958	1	Type 1 S/T protein phosphatase (PP1) catalytic subunit	Type 1 S/T protein phosphatase (PP1) catalytic subunit; involved in glycogen metabolism, sporulation and mitotic progression; interacts with multiple regulatory subunits; regulates actomyosin ring formation; subunit of CPF; recruited to mating projections by Afr1p interaction; regulates nucleocytoplasmic shuttling of Hxk2p; import into the nucleus is inhibited during spindle assembly checkpoint arrest; involved in dephosphorylating Rps6a/b and Bnr1p
S000000936	YER134C	""	""	437267	437803	-1	Magnesium-dependent acid phosphatase	Magnesium-dependent acid phosphatase; member of the haloacid dehalogenase superfamily; non-essential gene
S000000937	YER135C	""	""	437952	438344	-1	Putative protein of unknown function	Putative protein of unknown function; conserved among S. cerevisiae strains; YER135C is not an essential gene
S000000939	YER137C	""	""	441373	441819	-1	Protein of unknown function	Protein of unknown function
S000000941	YER139C	RTR1	Regulator of TRanscription	450563	451243	-1	Dual specificity protein phosphatase	Dual specificity protein phosphatase; dephosphorylates T1 and S5 in  C-terminal domain (CTD) of RNA polymerase II largest subunit, Rpo21p; autoregulates turnover of its mRNA; has a cysteine-rich motif required for function and conserved in eukaryotes; shuttles between the nucleus and cytoplasm; RTR1 has a paralog, RTR2, that arose from the whole genome duplication
S000000942	YER140W	EMP65	ER Membrane Protein of 65 kDa	451565	453235	1	Integral membrane protein of the ER	Integral membrane protein of the ER; forms an ER-membrane associated protein complex with Slp1p; identified along with SLP1 in a screen for mutants defective in the unfolded protein response (UPR); proposed to function in the folding of integral membrane proteins; interacts genetically with MPS3; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
S000000943	YER141W	COX15	Cytochrome c OXidase	453459	454919	1	Heme a synthase	Heme a synthase; required for the hydroxylation of heme O to form heme A, an essential prosthetic group for cytochrome c oxidase; oligomerization is required for function
S000000944	YER142C	MAG1	3-MethylAdenine DNA Glycosylase	455146	456036	-1	3-methyl-adenine DNA glycosylase	3-methyl-adenine DNA glycosylase; initiates base excision repair (BER) by removing damaged bases to create abasic sites that are subsequently repaired; expansion of BER repair removes alkylated bases resulting from alkylating agent-induced DNA damage, compensating for the lack of DNA repair by oxidative dealkylation; protein abundance increases in response to DNA replication stress
S000000945	YER143W	DDI1	DNA Damage Inducible	456319	457605	1	Aspartic-type endopeptidase	Aspartic-type endopeptidase; involved in the cleavage of long-chain polyubiquitinated substrates; role in proteasome-independent, ubiquitin (Ub)-dependent proteolysis and DNA-protein crosslink repair; Ub-shuttle receptor for HO and Ufo1p; DNA damage-inducible v-SNARE binding protein and negative regulator of constitutive exocytosis; possible role in the S-phase checkpoint; UBL-UBA family member containing Ub-like (UBL), helical, Ub-associated (UBA) and retroviral-like proteinase (RVP) domains
S000000946	YER144C	UBP5	UBiquitin-specific Protease	457806	460223	-1	Putative ubiquitin-specific protease	Putative ubiquitin-specific protease; concentrates at the bud neck; UBP5 has a paralog, DOA4, that arose from the whole genome duplication
S000000947	YER145C	FTR1	Fe TRansporter	460526	461740	-1	High affinity iron permease	High affinity iron permease; involved in the transport of iron across the plasma membrane; forms complex with Fet3p; expression is regulated by iron; protein abundance increases in response to DNA replication stress
S000000949	YER147C	SCC4	Sister Chromatid Cohesion	462968	464842	-1	Subunit of cohesin loading factor (Scc2p-Scc4p)	Subunit of cohesin loading factor (Scc2p-Scc4p); complex is required for the loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during double-strand break repair via phosphorylated histone H2AX
S000000951	YER149C	PEA2	PEAnut shmoo mutant	466208	467470	-1	Coiled-coil 12S polarisome subunit	Coiled-coil 12S polarisome subunit; required for polarity establishment, apical bud growth, shmoo formation, filamentous differentiation; involved in Bni1p localization at sites of polarized growth, controlling polarized assembly of actin cables; role in apical growth affects diploid-specific bipolar bud site selection; retains Slt2p at bud tip to regulate ER inheritance; role in Ca2+ influx, cell fusion; S288C allele encoding Leu409 rather than Met linked with non-invasion
S000000952	YER150W	SPI1	Stationary Phase Induced	468370	468816	1	GPI-anchored cell wall protein involved in weak acid resistance	GPI-anchored cell wall protein involved in weak acid resistance; basal expression requires Msn2p/Msn4p; expression is induced under conditions of stress and during the diauxic shift; SPI1 has a paralog, SED1, that arose from the whole genome duplication
S000000953	YER151C	UBP3	UBiquitin-specific Protease	469686	472424	-1	Ubiquitin-specific protease that cleaves Ub-protein fusions	Ubiquitin-specific protease that cleaves Ub-protein fusions; interacts with cofactor Bre5p to regulate transport between ER and Golgi, deubiquitinating COPII and COPI proteins, Sec23p and Sec27p; mediates steady-state retention of Golgi membrane proteins with Bre5p; phosphorylation (S695) by Hog1p regulates transcriptional activation by osmostress; inhibits Ras/PKA signaling through Ira2p; inhibits silencing; role in ribophagy; protein abundance increases in response to DNA replication stress
S000000954	YER152C	""	""	472657	473988	-1	Protein with 2-aminoadipate transaminase activity	Protein with 2-aminoadipate transaminase activity; shares amino acid similarity with the aminotransferases Aro8p and Aro9p; YER152C is not an essential gene
S000000955	YER153C	PET122	PETite colonies	474041	474805	-1	Mitochondrial translational activator specific for the COX3 mRNA	Mitochondrial translational activator specific for the COX3 mRNA; acts together with Pet54p and Pet494p; located in the mitochondrial inner membrane
S000000956	YER154W	OXA1	cytochrome OXidase Activity	475020	476228	1	Mitochondrial inner membrane insertase	Mitochondrial inner membrane insertase; mediates the insertion of both mitochondrial- and nuclear-encoded proteins from the matrix into the inner membrane; also has a role in insertion of carrier proteins into the inner membrane; acts as a voltage-gated ion channel, activated by substrate peptides; interacts with mitochondrial ribosomes; conserved from bacteria to animals
S000000957	YER155C	BEM2	Bud EMergence	476345	482848	-1	Rho GTPase activating protein (RhoGAP)	Rho GTPase activating protein (RhoGAP); contains RhoGAP activity specific for both Rho1p and Cdc42p, and potentially Rho4p; acts with the RhoGAP Bem3p to negatively regulate Cdc42p-dependent signal transduction; involved in the control of cytoskeleton organization and cellular morphogenesis; required for bud emergence and for the morphogenesis checkpoint in response to actin depolymerization; localizes to sites of polarized growth and the plasma membrane
S000000958	YER156C	MYG1	""	483325	484341	-1	Protein involved in mitochondrial function	Protein involved in mitochondrial function; interacts with Hsp82p and copurifies with Ipl1p; expression is copper responsive and downregulated in strains deleted for MAC1, a copper-responsive transcription factor; role in preventing L-A mycovirus pathogenesis; human homolog MYG1 is linked to developmental processes and dermatological diseases, complements yeast null mutant
S000000960	YER158C	""	""	488857	490578	-1	Protein of unknown function	Protein of unknown function; potentially phosphorylated by Cdc28p; YER158C has a paralog, AFR1, that arose from the whole genome duplication
S000000961	YER159C	BUR6	Bypass UAS Requirement	491530	491958	-1	Subunit of a heterodimeric NC2 transcription regulator complex	Subunit of a heterodimeric NC2 transcription regulator complex; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2alpha; complex also includes Ncb2p; bur6 ncb2 double mutation is functionally complemented by coexpression of human DRAP1 and DR1, although the single bur6 mutation is not complemented by its ortholog DRAP1
S000000963	YER161C	SPT2	SuPpressor of Ty's	499347	500348	-1	Protein involved in negative regulation of transcription	Protein involved in negative regulation of transcription; required for RNA polyadenylation; exhibits regulated interactions with both histones and SWI-SNF components; relocalizes to the cytosol in response to hypoxia; similar to mammalian HMG1 proteins
S000000964	YER162C	RAD4	RADiation sensitive	500630	502894	-1	Damaged-DNA binding subunit of Nuclear Excision Repair Factor 2	Damaged-DNA binding subunit of Nuclear Excision Repair Factor 2; recognizes and binds damaged DNA with Rad23p during nucleotide excision repair (NER); also involved with Rad23p in turnover of ubiquitylated proteins; Rad4p-Rad23p heterodimer binds to promoters of DNA damage response genes to repress their transcription in the absence of DNA damage; RAD4 and RAD51 pathways confer resistance to benzo[a]pyrene dihydrodiol
S000000965	YER163C	GCG1	Gamma-glutamyl Cyclotransferase acting on Glutathione	503084	503782	-1	Gamma-glutamyl cyclotransferase	Gamma-glutamyl cyclotransferase; cleaves the gamma-glutamyl bond of glutathione to yield 5-oxoproline and a Cys-Gly dipeptide; similar to mammalian pro-apoptotic protein ChaC1; expression of mouse ChaC1 in yeast increases apoptosis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; periodically expressed during the metabolic cycle
S000000966	YER164W	CHD1	Chromatin organization modifier, Helicase, and DNA-binding domains	505392	509798	1	Chromatin remodeler that regulates various aspects of transcription	Chromatin remodeler that regulates various aspects of transcription; acts in conjunction with Isw1b to regulate chromatin structure, maintain chromatin integrity during transcription elongation by RNAP II by preventing trans-histone exchange over coding regions; contains chromo domain, helicase domain and DNA-binding domain; component of both SAGA and SLIK complexes; in human, deletion of CHD1 is a frequent event in prostate cancer
S000000968	YER166W	DNF1	Drs2 Neo1 Family	512744	517459	1	Aminophospholipid translocase (flippase)	Aminophospholipid translocase (flippase); type 4 P-type ATPase; involved in phospholipid translocation, contributing to endocytosis, protein transport, and cellular polarization; localizes primarily to the plasma membrane; localizes to the shmoo tip where it has a redundant role in the cellular response to mating pheromone; DNF1 has a paralog, DNF2, that arose from the whole genome duplication
S000000969	YER167W	BCK2	Bypass of C Kinase	518216	520771	1	Serine/threonine-rich protein involved in PKC1 signaling pathway	Serine/threonine-rich protein involved in PKC1 signaling pathway; protein kinase C (PKC1) signaling pathway controls cell integrity; overproduction suppresses pkc1 mutations
S000000970	YER168C	CCA1	tRNA CCA-pyrophosphorylase	521029	522669	-1	ATP (CTP):tRNA-specific tRNA nucleotidyltransferase	ATP (CTP):tRNA-specific tRNA nucleotidyltransferase; different forms targeted to the nucleus, cytosol, and mitochondrion are generated via the use of multiple transcriptional and translational start sites; human homolog TRNT1 complements yeast null mutant
S000000971	YER169W	RPH1	Regulator of PHR1	523369	525759	1	JmjC domain-containing histone demethylase	JmjC domain-containing histone demethylase; targets tri- and dimethylated H3K36; associates with actively transcribed regions and promotes elongation; repressor of autophagy-related genes in nutrient-replete conditions; damage-responsive repressor of PHR1; phosphorylated by the Rad53p-dependent DNA damage checkpoint pathway and by a Rim1p-mediated event during starvation; target of stress-induced hormesis; RPH1 has a paralog, GIS1, that arose from the whole genome duplication
S000000972	YER170W	ADK2	ADenylate Kinase	525974	526651	1	Mitochondrial adenylate kinase	Mitochondrial adenylate kinase; catalyzes the reversible synthesis of GTP and AMP from GDP and ADP; may serve as a back-up for synthesizing GTP or ADP depending on metabolic conditions; 3' sequence of ADK2 varies with strain background
S000000975	YER173W	RAD24	RADiation sensitive	536300	538279	1	Checkpoint protein	Checkpoint protein; involved in the activation of the DNA damage and meiotic pachytene checkpoints; subunit of a clamp loader that loads Rad17p-Mec3p-Ddc1p onto DNA; homolog of human and S. pombe Rad17 protein
S000000976	YER174C	GRX4	GlutaRedoXin	538434	539168	-1	Glutathione-dependent oxidoreductase and glutathione S-transferase	Glutathione-dependent oxidoreductase and glutathione S-transferase; hydroperoxide/superoxide-radical responsive monothiol glutaredoxin subfamily member with Grx3p and Grx5p; protects cells from oxidative damage with GRX3 and GRX5; with Grx3p, promotes dissociation of Aft1p from iron regulon gene promoters and subsequent nuclear export in iron-replete conditions, regulating iron homeostasis; involved with Grx3p in Sir2p deglutathionylation, restoring deacetylase activity after disulfide stress
S000000977	YER175C	TMT1	Trans-aconitate MethylTransferase	539464	540363	-1	Trans-aconitate methyltransferase	Trans-aconitate methyltransferase; cytosolic enzyme that catalyzes the methyl esterification of 3-isopropylmalate, an intermediate of the leucine biosynthetic pathway, and trans-aconitate, which inhibits the citric acid cycle
S000000978	YER176W	ECM32	ExtraCellular Mutant	541690	545055	1	DNA dependent ATPase/DNA helicase	DNA dependent ATPase/DNA helicase; helicase belonging to the Dna2p- and Nam7p-like family of helicases that is involved in modulating translation termination; interacts with the translation termination factors, localized to polysomes
S000000979	YER177W	BMH1	Brain Modulosignalin Homolog	545611	546414	1	14-3-3 protein, major isoform	14-3-3 protein, major isoform; involved in post-transcriptional control of the proteome; binds to both proteins and to DNA, including replication origins; regulates exocytosis, vesicle transport, Ras/MAPK and rapamycin-sensitive signaling, aggresome formation, the spindle position checkpoint and meiotic commitment; protein abundance and relative distribution to the nucleus increase upon DNA replication stress; antiapoptotic gene; similar to several human 14-3-3 proteins
S000000980	YER178W	PDA1	Pyruvate Dehydrogenase Alpha	546817	548079	1	E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex	E1 alpha subunit of the pyruvate dehydrogenase (PDH) complex; catalyzes the direct oxidative decarboxylation of pyruvate to acetyl-CoA; phosphorylated; regulated by glucose; PDH complex is concentrated in spots within the mitochondrial matrix, often near the ERMES complex and near peroxisomes
S000000981	YER179W	DMC1	Disrupted Meiotic cDNA	548421	549517	1	Meiosis-specific recombinase required for double-strand break repair	Meiosis-specific recombinase required for double-strand break repair; also required for pairing between homologous chromosomes and for the normal morphogenesis of synaptonemal complex; binds ssDNA and dsDNA, forms helical filaments; potent inhibitor of the ATPase activity of  Srs2p helicase, blocking its ssDNA translocating motor activity and inhibiting its antirecombinase activity; stimulated by Rdh54p; homolog of Rad51p and the bacterial RecA protein 
S000000982	YER180C	ISC10	message Induced in Sporogenesis in S. Cerevisiae	549724	550527	-1	Meiosis-specific MAPK inhibitor	Meiosis-specific MAPK inhibitor; binds Smk1p during meiosis I; links APC/C-Ama1 to Smk1p activation; required for sporulation; transcript is induced 7.5 hr after induction of meiosis; Isc10p is ubiquitylated and degraded in an Ama1p-dependent manner upon completion of meiosis II
S000000983	YER181C	""	""	551473	551796	-1	Mitochondrial protein of unknown function	Mitochondrial protein of unknown function; conserved among S. cerevisiae strains; extensively overlaps a Ty1 LTR; YER181C is not an essential gene
S000000984	YER182W	FMP10	Found in Mitochondrial Proteome	552525	553259	1	Putative protein of unknown function	Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
S000000985	YER183C	FAU1	Folinic Acid Utilization	553334	553969	-1	5,10-methenyltetrahydrofolate synthetase	5,10-methenyltetrahydrofolate synthetase; involved in folic acid biosynthesis
S000000986	YER184C	TOG1	Transcriptional regulator of Oleate utilization Genes	556296	558680	-1	Transcriptional activator of oleate genes	Transcriptional activator of oleate genes; regulates genes involved in fatty acid utilization; zinc cluster protein; deletion confers sensitivity to Calcufluor white, and prevents growth on glycerol or lactate as sole carbon source
S000000987	YER185W	PUG1	Protoporphyrin Uptake Gene	559454	560365	1	Plasma membrane protein involved in protoprophyrin and heme transport	Plasma membrane protein involved in protoprophyrin and heme transport; roles in the uptake of protoprophyrin IX and the efflux of heme; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of proteins
S000000988	YER186C	""	""	561705	562625	-1	Putative protein of unknown function	Putative protein of unknown function
S000000989	YER187W	""	""	566230	566655	1	Putative protein of unknown function	Putative protein of unknown function; induced in respiratory-deficient cells
S000000990	YER188W	""	""	568040	568759	1	Putative protein of unknown function	Putative protein of unknown function; conserved across S. cerevisiae strains; large-scale analyses show mRNA expression increases under anaerobic conditions and two-hybrid interactions with Sst2p
S000000993	YHL001W	RPL14B	Ribosomal Protein of the Large subunit	104277	105091	1	Ribosomal 60S subunit protein L14B	Ribosomal 60S subunit protein L14B; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14B has a paralog, RPL14A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
S000000994	YHL002W	HSE1	Has Symptoms of class E mutants; resembles Hbp, Stam and East	102612	103970	1	Subunit of the endosomal Vps27p-Hse1p complex	Subunit of the endosomal Vps27p-Hse1p complex; complex is required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes
S000000995	YHL003C	LAG1	Longevity Assurance Gene	100648	101883	-1	Ceramide synthase component	Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lac1p; forms ER foci upon DNA replication stress; homolog of human CERS2, a tumor metastasis suppressor gene whose silencing enhances invasion/metastasis of prostate cancer cells; LAG1 has a paralog, LAC1, that arose from the whole genome duplication
S000000996	YHL004W	MRP4	Mitochondrial Ribosomal Protein	99219	100403	1	Mitochondrial ribosomal protein of the small subunit	Mitochondrial ribosomal protein of the small subunit
S000000997	YHL005C	""	""	98828	99220	-1	Putative protein of unknown function	Putative protein of unknown function; conserved among S. cerevisiae strains; YHL005C is not an essential gene
S000000998	YHL006C	SHU1	Suppressor of HU sensitivity	98343	98795	-1	Component of Shu complex (aka PCSS complex)	Component of Shu complex (aka PCSS complex); Shu complex also includes Psy3, Csm2, Shu2, and promotes error-free DNA repair, mediates inhibition of Srs2p function; essential for promoting the establishment of homolog bias during meiotic homologous recombination; promotes both crossover (CO) and non-crossover (NCO) pathways of meiotic recombination and formation of Rad51p filaments
S000000999	YHL007C	STE20	STErile	95118	97937	-1	Cdc42p-activated signal transducing kinase	Cdc42p-activated signal transducing kinase; involved in pheromone response, pseudohyphal/invasive growth, vacuole inheritance, down-regulation of sterol uptake; GBB motif binds Ste4p; member of the PAK (p21-activated kinase) family
S000001000	YHL008C	""	""	92627	94510	-1	Putative protein of unknown function	Putative protein of unknown function; may be involved in the uptake of chloride ions; does not appear to be involved in monocarboxylic acid transport; green fluorescent protein (GFP)-fusion protein localizes to the vacuole
S000001001	YHL009C	YAP3	Yeast AP-1	84068	85060	-1	Basic leucine zipper (bZIP) transcription factor	Basic leucine zipper (bZIP) transcription factor
S000001002	YHL010C	ETP1	Ethanol Tolerance Protein	81964	83721	-1	Protein of unknown function required for growth on ethanol	Protein of unknown function required for growth on ethanol; contains a zinc finger region and has homology to human BRAP2, which is a cytoplasmic protein that binds nuclear localization sequences
S000001003	YHL011C	PRS3	PhosphoRibosylpyrophosphate Synthetase	80654	81616	-1	5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase	5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase; synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes
S000001004	YHL012W	""	""	78936	80417	1	Putative UTP glucose-1-phosphate uridylyltransferase	Putative UTP glucose-1-phosphate uridylyltransferase; YHL012W has a paralog, UGP1, that arose from the whole genome duplication
S000001005	YHL013C	OTU2	member of the Ovarian TUmor family	77430	78353	-1	Protein of unknown function	Protein of unknown function; may interact with ribosomes, based on co-purification experiments; member of the ovarian tumor-like (OTU) superfamily of predicted cysteine proteases; shows cytoplasmic localization; protein abundance increases in response to DNA replication stress
S000001006	YHL014C	YLF2	""	76097	77314	-1	Protein of unknown function	Protein of unknown function; has weak similarity to E. coli GTP-binding protein gtp1; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
S000001007	YHL015W	RPS20	Ribosomal Protein of the Small subunit	75412	75777	1	Protein component of the small (40S) ribosomal subunit	Protein component of the small (40S) ribosomal subunit; overproduction suppresses mutations affecting RNA polymerase III-dependent transcription; homologous to mammalian ribosomal protein S20 and bacterial S10
S000001008	YHL016C	DUR3	Degradation of URea	72037	74244	-1	Plasma membrane transporter for both urea and polyamines	Plasma membrane transporter for both urea and polyamines; expression is highly sensitive to nitrogen catabolite repression and induced by allophanate, the last intermediate of the allantoin degradative pathway
S000001009	YHL017W	""	""	70276	71874	1	Putative protein of unknown function	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein co-localizes with clathrin-coated vesicles; YHL017W has a paralog, PTM1, that arose from the whole genome duplication
S000001011	YHL019C	APM2	clathrin Adaptor Protein complex Medium chain	67731	69548	-1	Protein of unknown function	Protein of unknown function; homologous to the medium chain of mammalian clathrin-associated protein complex; involved in vesicular transport
S000001012	YHL020C	OPI1	OverProducer of Inositol	66242	67456	-1	Transcriptional regulator of a variety of genes	Transcriptional regulator of a variety of genes; critical sensor of genotoxic stress; phosphorylation by protein kinase A stimulates Opi1p function in negative regulation of phospholipid biosynthetic genes; involved in telomere maintenance; null exhibits disrupted mitochondrial metabolism and low cardiolipin content, strongly correlated with overproduction of inositol; binds to phosphatidic acid
S000001013	YHL021C	AIM17	Altered Inheritance rate of Mitochondria	64462	65859	-1	Protein of unknown function	Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss
S000001014	YHL022C	SPO11	SPOrulation	62961	64157	-1	Meiosis-specific protein required for meiotic recombination	Meiosis-specific protein required for meiotic recombination; makes DNA double-strand breaks (DSB) during meiotic recombination, homolog pairing, and synaptonemal complex formation; role in meiotic DSB formation involves a topo-II-like transesterase mechanism; forms monotetrameric complex; covalently linked to DSBs through a 5'-phosphodiester bond between a catalytic tyrosine (Y135) and the DSB 5'- end; sequence similarity to a topo VI subunit from archaea, fission yeast rec12 and human SPO11
S000001015	YHL023C	NPR3	Nitrogen Permease Regulator	59123	62563	-1	Subunit of the Iml1p/SEACIT complex	Subunit of the Iml1p/SEACIT complex; SEACIT (Iml1p-Npr2p-Npr3p) is a subcomplex of SEAC, a coatomer-related complex that associates dynamically with the vacuole; Npr3p may have a structural or regulatory role, supporting Iml1p function as a GAP for the Rag family GTPase Gtr1p, and leading to inhibition of TORC1 signaling in response to amino acid deprivation; SEACIT is required for non-nitrogen-starvation-induced autophagy; null mutant has meiotic defects; human NPRL3 homolog
S000001016	YHL024W	RIM4	Regulator of IME2	56649	58790	1	Putative RNA-binding protein	Putative RNA-binding protein; involved in regulation of early and middle sporulation genes; forms amyloid-like aggregates under starvation that are active in translational repression
S000001017	YHL025W	SNF6	Sucrose NonFermenting	54851	55849	1	Subunit of the SWI/SNF chromatin remodeling complex	Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; functions interdependently in transcriptional activation with Snf2p and Snf5p; relocates to the cytosol under hypoxic conditions
S000001018	YHL026C	""	""	53219	54166	-1	Putative protein of unknown function	Putative protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; SWAT-GFP fusion protein localizes to the cell periphery, while mCherry fusion localizes to both the cell periphery and vacuole; YHL026C is not an essential gene; in 2005 the start site was moved 141 nt upstream (see Locus History)
S000001019	YHL027W	RIM101	Regulator of IME2	51111	52988	1	Cys2His2 zinc-finger transcriptional repressor	Cys2His2 zinc-finger transcriptional repressor; involved in alkaline responsive gene repression; involved in cell wall assembly; plays role in lager yeast flocculation under brewing conditions; required for alkaline pH-stimulated haploid invasive growth and sporulation; activated by alkaline-dependent proteolytic processing which results in removal of the C-terminal tail; similar to A. nidulans PacC
S000001020	YHL028W	WSC4	cell Wall integrity and Stress response Component	48763	50580	1	Endoplasmic reticulum (ER) membrane protein	Endoplasmic reticulum (ER) membrane protein; involved in the translocation of soluble secretory proteins and insertion of membrane proteins into the ER membrane; may also have a role in the stress response but has only partial functional overlap with WSC1-3
S000001021	YHL029C	OCA5	Oxidant-induced Cell-cycle Arrest	45929	47968	-1	Cytoplasmic protein required for replication of Brome mosaic virus	Cytoplasmic protein required for replication of Brome mosaic virus; S. cerevisiae is a model system for studying replication of positive-strand RNA viruses in their natural hosts
S000001022	YHL030W	ECM29	ExtraCellular Mutant	40084	45690	1	Scaffold protein	Scaffold protein; assists in association of proteasome core particle with regulatory particle; inhibitor of proteasome in vivo and in vitro; inhibits proteasomal ATPase activity; degraded by the mature proteasome after assembly; contains HEAT-like repeats; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress
S000001023	YHL031C	GOS1	GOlgi Snare	38815	39486	-1	v-SNARE protein involved in Golgi transport	v-SNARE protein involved in Golgi transport; homolog of the mammalian protein GOS-28/GS28
S000001024	YHL032C	GUT1	Glycerol UTilization	36379	38508	-1	Glycerol kinase	Glycerol kinase; converts glycerol to glycerol-3-phosphate; glucose repression of expression is mediated by Adr1p and Ino2p-Ino4p; derepression of expression on non-fermentable carbon sources is mediated by Opi1p and Rsf1p
S000001025	YHL033C	RPL8A	Ribosomal Protein of the Large subunit	35255	36025	-1	Ribosomal 60S subunit protein L8A	Ribosomal 60S subunit protein L8A; required for processing of 27SA3 pre-rRNA to 27SB during assembly of large subunit; binds Domain I of 25S, 5.8 S rRNAs; mutation results in decreased amounts of free 60S subunits; RPL8A has paralog RPL8B from whole genome duplication; RPL8A or RPL8B ribosomes not functionally interchangeable; proportions of Rpl8ap, Rpl8bp in 80S ribosome differ in glucose and glycerol; homologous to mammalian ribosomal protein L7A, no bacterial homolog
S000001026	YHL034C	SBP1	""	33193	34077	-1	Protein with roles in translation repression and P-body disassembly	Protein with roles in translation repression and P-body disassembly; binds eIF4G; has an RGG motif; found in cytoplasmic P bodies; binds to mRNAs under glucose starvation stress, most often in the 5' UTR; found associated with small nucleolar RNAs snR10 and snR11; SBP1 has a paralog, RNP1, that arose from the whole genome duplication
S000001027	YHL035C	VMR1	Vacuolar Multidrug Resistance	27978	32756	-1	Vacuolar membrane protein	Vacuolar membrane protein; involved in multiple drug resistance and metal sensitivity; ATP-binding cassette (ABC) family member involved in drug transport; potential Cdc28p substrate; induced under respiratory conditions; VMR1 has a paralog, YBT1, that arose from the whole genome duplication
S000001028	YHL036W	MUP3	Methionine UPtake	26241	27881	1	Low affinity methionine permease	Low affinity methionine permease; similar to Mup1p
S000001029	YHL037C	""	""	25778	26179	-1	Putative protein of unknown function	Putative protein of unknown function; conserved among S. cerevisiae strains
S000001030	YHL038C	CBP2	Cytochrome B mRNA Processing	23617	25509	-1	Required for splicing of the group I intron bI5 of the COB pre-mRNA	Required for splicing of the group I intron bI5 of the COB pre-mRNA; nuclear-encoded mitochondrial protein that binds to the RNA to promote splicing; also involved in but not essential for splicing of the COB bI2 intron and the intron in the 21S rRNA gene
S000001031	YHL039W	EFM1	Elongation Factor Methyltransferase	21783	23540	1	Lysine methyltransferase	Lysine methyltransferase; involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
S000001032	YHL040C	ARN1	AFT1 ReguloN	19088	20971	-1	ARN family transporter for siderophore-iron chelates	ARN family transporter for siderophore-iron chelates; responsible for uptake of iron bound to ferrirubin, ferrirhodin, and related siderophores; protein increases in abundance and relocalizes to the vacuole upon DNA replication stress
S000001033	YHL041W	""	""	17392	17841	1	Putative protein of unknown function	Putative protein of unknown function; conserved across S. cerevisiae strains
S000001034	YHL042W	""	""	15667	16119	1	Endoplasmic reticulum protein	Endoplasmic reticulum protein; member of DUP380 subfamily of conserved, often subtelomerically-encoded proteins; may contribute to maintenance of ER and nuclear morphology; null mutants have distorted nuclear envelope shape, and discontinuous, misshapen perinuclear ER
S000001035	YHL043W	ECM34	ExtraCellular Mutant	14901	15413	1	Protein of unknown function	Protein of unknown function; member of the DUP380 subfamily of conserved, often subtelomerically-encoded proteins; SWAT-GFP, seamless-GFP and mCherry C-terminal fusion proteins localize to the cytosol
S000001036	YHL044W	DFP4	DUP240 Family Protein	13565	14272	1	Putative integral membrane protein	Putative integral membrane protein; member of DUP240 gene family; green fluorescent protein (GFP)-fusion protein localizes to the plasma membrane in a punctate pattern
S000001037	YHL045W	PXP3	PeroXisomal Protein	12502	12849	1	Peroxisomal protein of unknown function	Peroxisomal protein of unknown function; last 10 C-terminal amino acids contains a novel PTS1 motif, both necessary and sufficient for Pex5p-dependent peroxisomal targeting; also localizes to the nucleus; PXP3 is not an essential gene
S000001038	YHL046C	PAU13	seriPAUperin	11923	12285	-1	Protein of unknown function	Protein of unknown function; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expression is induced after ethanol shock; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum and vacuole respectively
S000001039	YHL047C	ARN2	AFT1 ReguloN	8351	10213	-1	Transporter	Transporter; member of the ARN family of transporters that specifically recognize siderophore-iron chelates; responsible for uptake of iron bound to the siderophore triacetylfusarinine C
S000001043	YHR001W	OSH7	OxySterol binding protein Homolog	106055	107368	1	Oxysterol-binding phosphatidylserine transferase	Oxysterol-binding phosphatidylserine transferase; part of family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability; OSH7 has a paralog, OSH6, that arose from the whole genome duplication
S000001045	YHR003C	TCD1	tRNA ThreonylCarbamoyladenosine Dehydratase	110028	111317	-1	tRNA threonylcarbamoyladenosine dehydratase	tRNA threonylcarbamoyladenosine dehydratase; required for the ct6A tRNA base modification, where an adenosine at position 37 is modified to form a cyclized active ester with an oxazolone ring; localized to the mitochondrial outer membrane; TCD1 has a paralog, TCD2, that arose from the whole genome duplication
S000001046	YHR004C	NEM1	Nuclear Envelope Morphology	111754	113094	-1	Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme	Probable catalytic subunit of Nem1p-Spo7p phosphatase holoenzyme; regulates nuclear/ER membrane association and activation of Pah1p, a phosphatidate phosphatase involved in the production of diacylglycerol DAG for lipid droplet biogenesis; regulates nuclear growth by controlling phospholipid biosynthesis; required for normal nuclear envelope morphology and sporulation; phosphatase activity of the Nem1p-Spo7p complex is inhibited by Ice2p; homolog of the human protein Dullard
S000001047	YHR005C	GPA1	G Protein Alpha subunit	113499	114917	-1	Subunit of G protein involved in pheromone response	Subunit of G protein involved in pheromone response; GTP-binding alpha subunit of heterotrimeric G protein; negatively regulates mating pathway by sequestering G(beta)gamma and triggering an adaptive response; activates Vps34p at endosome; protein abundance increases in response to DNA replication stress; mutations in human homolog GNAS associated with McCune-Albright syndrome
S000001048	YHR006W	STP2	protein with similarity to Stp1p	117814	119439	1	Transcription factor	Transcription factor; activated by proteolytic processing in response to signals from the SPS sensor system for external amino acids; activates transcription of amino acid permease genes; STP2 has a paralog, STP1, that arose from the whole genome duplication
S000001049	YHR007C	ERG11	ERGosterol biosynthesis	120091	121683	-1	Lanosterol 14-alpha-demethylase	Lanosterol 14-alpha-demethylase; catalyzes C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in ergosterol biosynthesis pathway; transcriptionally down-regulated when ergosterol is in excess; member of cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p; human CYP51A1 functionally complements the lethality of the erg11 null mutation
S000001050	YHR008C	SOD2	SuperOxide Dismutase	122889	123590	-1	Mitochondrial manganese superoxide dismutase	Mitochondrial manganese superoxide dismutase; protects cells against oxygen toxicity and oxidative stress; human mitochondrial SOD2 can complement a yeast null mutant and human cytoplasmic SOD1 can also complement when targeted to the mitochondrial matrix
S000001051	YHR009C	TDA3	Topoisomerase I Damage Affected	124109	125680	-1	Putative oxidoreductase involved in late endosome to Golgi transport	Putative oxidoreductase involved in late endosome to Golgi transport; physical and genetical interactions with Btn2p; null mutant is viable, has extended S phase, and sensitive to expression of top1-T722A allele; similar to human FOXRED1
S000001052	YHR010W	RPL27A	Ribosomal Protein of the Large subunit	126521	127492	1	Ribosomal 60S subunit protein L27A	Ribosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication
S000001053	YHR011W	DIA4	Digs Into Agar	127780	129120	1	Probable mitochondrial seryl-tRNA synthetase	Probable mitochondrial seryl-tRNA synthetase; mutant displays increased invasive and pseudohyphal growth
S000001054	YHR012W	VPS29	Vacuolar Protein Sorting	129481	130448	1	Subunit of the membrane-associated retromer complex	Subunit of the membrane-associated retromer complex; endosomal protein; essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval
S000001055	YHR013C	ARD1	ARrest Defective	130730	131446	-1	Subunit of protein N-terminal acetyltransferase NatA	Subunit of protein N-terminal acetyltransferase NatA; NatA comprises Nat1p, Ard1p, Nat5p; acetylates many proteins to influence telomeric silencing, cell cycle, heat-shock resistance, mating, sporulation, early stages of mitophagy; protein abundance increases under DNA replication stress; mutations in human homolog X-linked NAA10 lead to Ogden syndrome (S37P) and intellectual disability (R116W); expression of human NAA10 and NAA15 can complement ard1 nat1 double mutant
S000001056	YHR014W	SPO13	SPOrulation	132047	132922	1	Meiotic regulator	Meiotic regulator; involved in maintaining sister chromatid cohesion during meiosis I as well as promoting proper attachment of kinetochores to the spindle during meiosis I and meiosis II; anaphase-promoting complex (APC) substrate that is degraded during anaphase I; expressed only in meiotic cells
S000001057	YHR015W	MIP6	Mex67-Interacting Protein	134554	136533	1	mRNA-binding protein	mRNA-binding protein; interacts with the UBA domain of mRNA export factor Mex67p; participates in the export of Msn2/4 stress-dependent mRNAs; partially redundant function with paralog PES4 in the steady-state expression, translational timing and localization of a subset of Ndt80-dependent mRNAs that are protected until translated near the end of meiosis II; redundant role with PES4 in sporulation; shuttles between the nucleus and cytoplasm, localizes to stress granules and the prospore membrane
S000001058	YHR016C	YSC84	""	136881	138455	-1	Actin-binding protein	Actin-binding protein; involved in bundling of actin filaments and endocytosis of actin cortical patches; activity stimulated by Las17p; contains SH3 domain similar to Rvs167p; YSC84 has a paralog, LSB3, that arose from the whole genome duplication
S000001059	YHR017W	YSC83	""	138694	139851	1	Non-essential mitochondrial protein of unknown function	Non-essential mitochondrial protein of unknown function; mRNA induced during meiosis, peaking between mid to late prophase of meiosis I; similar to S. douglasii YSD83
S000001060	YHR018C	ARG4	ARGinine requiring	140011	141402	-1	Argininosuccinate lyase	Argininosuccinate lyase; catalyzes the final step in the arginine biosynthesis pathway
S000001063	YHR021C	RPS27B	Ribosomal Protein of the Small subunit	147871	148669	-1	Protein component of the small (40S) ribosomal subunit	Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27B has a paralog, RPS27A, that arose from the whole genome duplication
S000001064	YHR022C	""	""	149575	150345	-1	Putative protein of unknown function	Putative protein of unknown function; YHR022C is not an essential gene
S000001067	YHR025W	THR1	THReonine requiring	159438	160511	1	Homoserine kinase	Homoserine kinase; conserved protein required for threonine biosynthesis; long-lived protein that is preferentially retained in mother cells and forms cytoplasmic filaments; expression is regulated by the GCN4-mediated general amino acid control pathway
S000001068	YHR026W	VMA16	Vacuolar Membrane Atpase	160844	161485	1	Subunit c'' of the vacuolar ATPase	Subunit c'' of the vacuolar ATPase; v-ATPase functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain
S000001070	YHR028C	DAP2	Dipeptidyl AminoPeptidase	164978	167434	-1	Dipeptidyl aminopeptidase	Dipeptidyl aminopeptidase; synthesized as a glycosylated precursor; localizes to the vacuolar membrane; similar to Ste13p
S000001071	YHR029C	YHI9	""	167677	168561	-1	Protein of unknown function	Protein of unknown function; null mutant is defective in unfolded protein response; possibly involved in a membrane regulation metabolic pathway; member of the PhzF superfamily, though most likely not involved in phenazine production
S000001072	YHR030C	SLT2	Suppressor of the LyTic phenotype	168890	170344	-1	Serine/threonine MAP kinase	Serine/threonine MAP kinase; coordinates expression of all 19S regulatory particle assembly-chaperones (RACs) to control proteasome abundance; involved in regulating maintenance of cell wall integrity, cell cycle progression, nuclear mRNA retention in heat shock, septum assembly; required for mitophagy, pexophagy; affects recruitment of mitochondria to phagophore assembly site; plays role in adaptive response of cells to cold; regulated by the PKC1-mediated signaling pathway
S000001073	YHR031C	RRM3	rDNA Recombination Mutation	170799	172970	-1	DNA helicase involved in rDNA replication and Ty1 transposition	DNA helicase involved in rDNA replication and Ty1 transposition; binds to and suppresses DNA damage at G4 motifs in vivo; relieves replication fork pauses at telomeric regions; deletion or inactivation of helicase function suppresses chromosome instability (CIN) associated with centromere or kinetochore defects; structurally and functionally related to Pif1p
S000001074	YHR032W	ERC1	Ethionine Resistance Conferring	173344	175089	1	Member of the multi-drug and toxin extrusion (MATE) family	Member of the multi-drug and toxin extrusion (MATE) family; the MATE family is part of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily; overproduction confers ethionine resistance and accumulation of S-adenosylmethionine
S000001075	YHR033W	""	""	175548	176819	1	Putative protein of unknown function	Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm; YHR033W has a paralog, PRO1, that arose from the whole genome duplication
S000001076	YHR034C	PIH1	Protein Interacting with Hsp90	176965	177999	-1	Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1)	Component of the conserved R2TP complex (Rvb1-Rvb2-Tah1-Pih1); R2TP complex interacts with Hsp90 (Hsp82p and Hsc82p) to mediate assembly large protein complexes such as box C/D snoRNPs and RNA polymerase II
S000001077	YHR035W	NEL1	Non-ERES Localized Sec23 homolog	178219	180111	1	Activator of Sar1p GTPase activity	Activator of Sar1p GTPase activity; paralog of Sec23 but does not associate with the COPII components; not an essential gene
S000001078	YHR036W	BRL1	BRr6 Like protein	180345	181760	1	Essential nuclear envelope/ER integral membrane protein	Essential nuclear envelope/ER integral membrane protein; interacts and functions with Apq12p and Brr6p in lipid homeostasis; mutants are defective in nuclear pore complex biogenesis, mRNA nuclear export and are sensitive to sterol biosynthesis inhibitors and membrane fluidizing agents; identified as a dosage suppressor of a temperature sensitive mutation in the major karyopherin, CRM1; homologous to Brr6p
S000001079	YHR037W	PUT2	Proline UTilization	181977	183704	1	Delta-1-pyrroline-5-carboxylate dehydrogenase	Delta-1-pyrroline-5-carboxylate dehydrogenase; nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; deficiency of human homolog ALDH4A1 causes type II hyperprolinemia (HPII), an autosomal recessive inborn error of metabolism; human homolog ALDH4A1 can complement yeast null mutant
S000001080	YHR038W	RRF1	Ribosome Recycling Factor	184066	184758	1	Mitochondrial ribosome recycling factor	Mitochondrial ribosome recycling factor; essential for mitochondrial protein synthesis and for the maintenance of the respiratory function of mitochondria
S000001081	YHR039C	MSC7	Meiotic Sister-Chromatid recombination	184875	186809	-1	Protein of unknown function	Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; msc7 mutants are defective in directing meiotic recombination events to homologous chromatids
S000001082	YHR040W	BCD1	Box C/D snoRNA accumulation	187924	189024	1	Essential protein required for the accumulation of box C/D snoRNA	Essential protein required for the accumulation of box C/D snoRNA
S000001083	YHR041C	SRB2	Suppressor of RNA polymerase B	189131	189864	-1	Subunit of the RNA polymerase II mediator complex	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; general transcription factor involved in telomere maintenance
S000001084	YHR042W	NCP1	NADP-Cytochrome P450 reductase	190543	192618	1	NADP-cytochrome P450 reductase	NADP-cytochrome P450 reductase; involved in ergosterol biosynthesis; associated and coordinately regulated with Erg11p
S000001085	YHR043C	DOG2	DeOxyGlucose	192805	193545	-1	2-deoxyglucose-6-phosphate phosphatase	2-deoxyglucose-6-phosphate phosphatase; member of a family of low molecular weight phosphatases, induced by oxidative and osmotic stress, confers 2-deoxyglucose resistance when overexpressed; DOG2 has a paralog, DOG1, that arose from a single-locus duplication; the last half of DOG1 and DOG2 are subject to gene conversions among S. cerevisiae, S. paradoxus, and S. mikatae
S000001086	YHR044C	DOG1	DeOxyGlucose	194068	194808	-1	2-deoxyglucose-6-phosphate phosphatase	2-deoxyglucose-6-phosphate phosphatase; member of a family of low molecular weight phosphatases; confers 2-deoxyglucose resistance when overexpressed; DOG1 has a paralog, DOG2, that arose from a single-locus duplication; the last half of DOG1 and DOG2 are subject to gene conversions among S. cerevisiae, S. paradoxus, and S. mikatae
S000001087	YHR045W	DDE1	Dap1-related DNA damage response and Ergosterol biosynthesis protein	195551	197233	1	Protein involved in DNA damage response and ergosterol biosynthesis	Protein involved in DNA damage response and ergosterol biosynthesis; acts in cooperation with Dap1p in regulating DNA damage response and ergosterol biosynthesis
S000001088	YHR046C	INM1	INositol Monophosphatase	197398	198285	-1	Inositol monophosphatase	Inositol monophosphatase; involved in biosynthesis of inositol and in phosphoinositide second messenger signaling; INM1 expression increases in the presence of inositol and decreases upon exposure to antibipolar drugs lithium and valproate
S000001089	YHR047C	AAP1	Arginine/alanine AminoPeptidase	198740	201310	-1	Arginine/alanine amino peptidase	Arginine/alanine amino peptidase; overproduction stimulates glycogen accumulation; AAP1 has a paralog, APE2, that arose from the whole genome duplication
S000001090	YHR048W	YHK8	""	204607	206151	1	Presumed antiporter of the major facilitator superfamily	Presumed antiporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; expression of gene is up-regulated in cells exhibiting reduced susceptibility to azoles
S000001091	YHR049W	FSH1	Family of Serine Hydrolases	206462	207193	1	Lysophospholipase	Lysophospholipase; hydrolyzes lysophosphatidylserine to release free fatty acid; involved in regulated cell death; localizes to both the nucleus and cytoplasm; contains a catalytic triad of Ser-His-Asp that is part of an alpha/beta hydrolase fold and a lipase motif (GXSXG); sequence similarity to Fsh2p and Fsh3p and the human candidate tumor suppressor and serine hydrolase, OVCA2 
S000001092	YHR050W	SMF2	Suppressor of Mitochondria import Function	207654	209303	1	Divalent metal ion transporter involved in manganese homeostasis	Divalent metal ion transporter involved in manganese homeostasis; has broad specificity for di-valent and tri-valent metals; post-translationally regulated by levels of metal ions; member of the Nramp family of metal transport proteins
S000001093	YHR051W	COX6	Cytochrome c OXidase	209705	210151	1	Subunit VI of cytochrome c oxidase (Complex IV)	Subunit VI of cytochrome c oxidase (Complex IV); Complex IV is the terminal member of the mitochondrial inner membrane electron transport chain; expression is regulated by oxygen levels
S000001099	YHR057C	CPR2	Cyclosporin A-sensitive Proline Rotamase	218226	218843	-1	Peptidyl-prolyl cis-trans isomerase (cyclophilin)	Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; potential role in the secretory pathway; seamless-GFP and mCherry fusion proteins localize to the vacuole, while SWAT-GFP fusion localizes to both the endoplasmic reticulum and vacuole; suppresses toxicity of slow-folding human Z-type alpha1-antitrypsin variant associated with liver cirrhosis and emphysema
S000001100	YHR058C	MED6	MEDiator complex	218997	219884	-1	Subunit of the RNA polymerase II mediator complex	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress
S000001101	YHR059W	FYV4	Function required for Yeast Viability	220108	220500	1	Protein of unknown function	Protein of unknown function; required for survival upon exposure to K1 killer toxin
S000001102	YHR060W	VMA22	Vacuolar Membrane Atpase	220725	221270	1	Protein that is required for vacuolar H+-ATPase (V-ATPase) function	Protein that is required for vacuolar H+-ATPase (V-ATPase) function; peripheral membrane protein; not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER)
S000001103	YHR061C	GIC1	GTPase Interactive Component	221534	222478	-1	Rho-family GTPase interacting protein and Cdc42p effector	Rho-family GTPase interacting protein and Cdc42p effector; required with Gic2p for polarity establishment and initiation of budding; involved in recruitment of septins to the presumptive bud site; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain; gic1 gic2 double mutants are defective for polarizing Cdc42p at elevated temperature, so may function both up- and downstream of Cdc42p; regulates mitotic exit; relocalizes from bud neck to nucleus upon DNA replication stress
S000001104	YHR062C	RPP1	Ribonuclease P Protein	222877	223758	-1	Subunit of both RNase MRP and nuclear RNase P	Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; relocalizes to the cytosol in response to hypoxia
S000001105	YHR063C	PAN5	PANtothenate biosynthesis	224030	225169	-1	2-dehydropantoate 2-reductase	2-dehydropantoate 2-reductase; part of the pantothenic acid pathway, structurally homologous to E. coli panE
S000001106	YHR064C	SSZ1	""	225525	227141	-1	Hsp70 protein that interacts with Zuo1p (a DnaJ homolog)	Hsp70 protein that interacts with Zuo1p (a DnaJ homolog); interacts with Zuo1p to form a ribosome-associated complex that binds the ribosome via the Zuo1p subunit; also involved in pleiotropic drug resistance via sequential activation of PDR1 and PDR5; binds ATP
S000001107	YHR065C	RRP3	Ribosomal RNA Processing	227532	229037	-1	Protein involved in rRNA processing	Protein involved in rRNA processing; required for maturation of the 35S primary transcript of pre-rRNA and for cleavage leading to mature 18S rRNA; homologous to eIF-4a, which is a DEAD box RNA-dependent ATPase with helicase activity
S000001108	YHR066W	SSF1	Suppressor of ste4 (Four)	229335	230696	1	Constituent of 66S pre-ribosomal particles	Constituent of 66S pre-ribosomal particles; required for ribosomal large subunit maturation; functionally redundant with Ssf2p; member of the Brix family; SSF1 has a paralog, SSF2, that arose from the whole genome duplication
S000001109	YHR067W	HTD2	Hydroxyacyl-Thioester Dehydratase	230970	231812	1	Mitochondrial 3-hydroxyacyl-thioester dehydratase	Mitochondrial 3-hydroxyacyl-thioester dehydratase; involved in fatty acid biosynthesis, required for respiratory growth and for normal mitochondrial morphology
S000001112	YHR070W	TRM5	tRNA Methyltransferase	234881	236380	1	tRNA(m(1)G37)methyltransferase	tRNA(m(1)G37)methyltransferase; methylates a tRNA base adjacent to the anticodon that has a role in prevention of frameshifting; localized to both cytoplasm and mitochondria, and modifies both cytoplasmic and mitochondrial tRNAs; mutations in human ortholog TRMT5 are associated with skeletal muscle respiratory chain deficiencies, and trm5 mutations analogous to disease mutations decrease respiration
S000001113	YHR071W	PCL5	Pho85 CycLin	237004	237693	1	Cyclin	Cyclin; interacts with and phosphorylated by Pho85p cyclin-dependent kinase (Cdk), induced by Gcn4p at level of transcription, specifically required for Gcn4p degradation, may be sensor of cellular protein biosynthetic capacity
S000001115	YHR073W	OSH3	OxySterol binding protein Homolog	242582	245572	1	Member of an oxysterol-binding protein family	Member of an oxysterol-binding protein family; this family has seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability; contains FFAT motif; interacts with ER anchor Scs2p at patches at the plasma membrane; regulated by sterol binding
S000001117	YHR075C	PPE1	Phosphoprotein Phosphatase methylEsterase	248439	249641	-1	Protein with carboxyl methyl esterase activity	Protein with carboxyl methyl esterase activity; may have a role in demethylation of the phosphoprotein phosphatase catalytic subunit; also identified as a small subunit mitochondrial ribosomal protein
S000001118	YHR076W	PTC7	Phosphatase type Two C	251101	252225	1	Type 2C serine/threonine protein phosphatase (PP2C)	Type 2C serine/threonine protein phosphatase (PP2C); alternatively spliced to create two mRNA isoforms; protein from spliced form localizes to the mitochondria while the one from the unspliced form is localized to the nuclear envelope; activates coenzyme Q6 biosynthesis by dephosphorylation of demethoxy-Q6 hydroxylase Coq7p
S000001119	YHR077C	NMD2	Nonsense-mediated MRNA Decay	252374	255756	-1	Protein involved in the nonsense-mediated mRNA decay (NMD) pathway	Protein involved in the nonsense-mediated mRNA decay (NMD) pathway; interacts with Nam7p and Upf3p; involved in telomere maintenance
S000001120	YHR078W	""	""	256360	258018	1	High osmolarity-regulated gene of unknown function	High osmolarity-regulated gene of unknown function
S000001121	YHR079C	IRE1	Inositol REquiring	258244	261591	-1	Serine-threonine kinase and endoribonuclease	Serine-threonine kinase and endoribonuclease; transmembrane protein that mediates the unfolded protein response (UPR) by regulating Hac1p synthesis through HAC1 mRNA splicing; role in homeostatic adaptation to ER stress; Kar2p binds inactive Ire1p and releases from it upon ER stress
S000001122	YHR080C	LAM4	Lipid transfer protein Anchored at Membrane contact sites	262801	266838	-1	Sterol-binding protein that localizes to puncta in the cortical ER	Sterol-binding protein that localizes to puncta in the cortical ER; sterol binding occurs via two StART-like domains; one of six StART-like domain-containing proteins in yeast that may be involved in intracellular sterol transfer between membranes; conserved across eukaryotes; has both GRAM and StART-like (VASt) domains; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
S000001123	YHR081W	LRP1	Like RrP6	267538	268092	1	Nuclear exosome-associated nucleic acid binding protein	Nuclear exosome-associated nucleic acid binding protein; involved in RNA processing, surveillance, degradation, tethering, and export; forms a stable heterodimer with Rrp6p and regulates its exonucleolytic activity; rapidly degraded by the proteasome in the absence of Rrp6p; homolog of mammalian nuclear matrix protein C1D involved in regulation of DNA repair and recombination
S000001124	YHR082C	KSP1	Kinase Suppressing Prp20-10	268459	271548	-1	Serine/threonine protein kinase	Serine/threonine protein kinase; associates with TORC1 and likely involved in TOR signaling cascades; negative regulator of autophagy; nuclear translocation required for haploid filamentous growth; regulates filamentous growth induced nuclear translocation of Bcy1p, Fus3p, and Sks1p; overproduction causes allele-specific suppression of prp20-10; protein abundance increases in response to DNA replication stress
S000001125	YHR083W	SAM35	Sorting and Assembly Machinery	272627	273616	1	Component of the sorting and assembly machinery (SAM) complex	Component of the sorting and assembly machinery (SAM) complex; the SAM (or TOB) complex is located in the mitochondrial outer membrane; the complex binds precursors of beta-barrel proteins and facilitates their insertion into the outer membrane
S000001127	YHR085W	IPI1	Involved in Processing ITS2	276764	277768	1	Component of the Rix1 complex and possibly pre-replicative complexes	Component of the Rix1 complex and possibly pre-replicative complexes; required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-replicative complex (pre-RC) formation and maintenance during DNA replication licensing; relocalizes to the cytosol in response to hypoxia; essential gene
S000001128	YHR086W	NAM8	Nuclear Accommodation of Mitochondria	278153	279724	1	RNA binding protein, component of the U1 snRNP protein	RNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis-specific splicing of REC107 pre-mRNA; Nam8p regulon embraces the meiotic pre-mRNAs of REC107, HFM1, AMA1 SPO22 and PCH2; the putative RNA binding domains RRM2 and RRM3 are required for Nam8p meiotic function
S000001129	YHR087W	RTC3	Restriction of Telomere Capping	280820	281155	1	Protein of unknown function involved in RNA metabolism	Protein of unknown function involved in RNA metabolism; has structural similarity to SBDS, the human protein mutated in Shwachman-Diamond Syndrome (the yeast SBDS ortholog is SDO1); null mutation suppresses cdc13-1 temperature sensitivity; protein abundance increases in response to DNA replication stress
S000001132	YHR090C	YNG2	Yeast iNG1 homolog	283777	284625	-1	Subunit of NuA4, an essential histone acetyltransferase complex	Subunit of NuA4, an essential histone acetyltransferase complex; positions Piccolo NuA4 for efficient acetylation of histone H4 or histone H2A; relocalizes to the cytosol in response to hypoxia; similar to human tumor suppressor ING1 and its isoforms ING4 and ING5
S000001133	YHR091C	MSR1	Mitochondrial tRNA Synthetase aRginine	284839	286770	-1	Mitochondrial arginyl-tRNA synthetase	Mitochondrial arginyl-tRNA synthetase; mutations in human ortholog are associated with pontocerebellar hypoplasia type 6; MSR1 has a paralog, YDR341C, that arose from the whole genome duplication
S000001134	YHR092C	HXT4	HeXose Transporter	287081	288811	-1	High-affinity glucose transporter	High-affinity glucose transporter; member of the major facilitator superfamily, expression is induced by low levels of glucose and repressed by high levels of glucose; HXT4 has a paralog, HXT7, that arose from the whole genome duplication
S000001135	YHR093W	AHT1	""	289142	289690	1	Putative protein of unknown function	Putative protein of unknown function; conserved across S. cerevisiae strains; not conserved in closely related Saccharomyces species; multicopy suppressor of glucose transport defects, likely due to the presence of an HXT4 regulatory element in the region
S000001136	YHR094C	HXT1	HeXose Transporter	290913	292625	-1	Low-affinity glucose transporter of the major facilitator superfamily	Low-affinity glucose transporter of the major facilitator superfamily; expression is induced by Hxk2p in the presence of glucose and repressed by Rgt1p when glucose is limiting; HXT1 has a paralog, HXT6, what arose from the whole genome duplication
S000001137	YHR095W	""	""	292943	293437	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000001138	YHR096C	HXT5	HeXose Transporter	294669	296447	-1	Hexose transporter with moderate affinity for glucose	Hexose transporter with moderate affinity for glucose; induced in the presence of non-fermentable carbon sources, induced by a decrease in growth rate, contains an extended N-terminal domain relative to other HXTs; HXT5 has a paralog, HXT3, that arose from the whole genome duplication
S000001139	YHR097C	PAL2	Pears And Lemons	297385	298609	-1	Protein involved in clathrin-mediated endocytosis	Protein involved in clathrin-mediated endocytosis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; YHR097C has a paralog, PAL1, that arose from the whole genome duplication
S000001140	YHR098C	SFB3	Sed Five Binding	299145	301934	-1	Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat	Component of the Sec23p-Sfb3p heterodimer of the COPII vesicle coat; COPII coat is required for cargo selection during vesicle formation in ER to Golgi transport; scaffolding function of Lst1p required to generate vesicles that can accommodate difficult cargo proteins that include large oligomeric assemblies and asymmetrically distributed membrane proteins; with Atg40p acts in autophagy of the ER; homologous to Sec24p and Sfb2p
S000001142	YHR100C	GEP4	GEnetic interactors of Prohibitins	314116	314673	-1	Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase)	Mitochondrial phosphatidylglycerophosphatase (PGP phosphatase); dephosphorylates phosphatidylglycerolphosphate to generate phosphatidylglycerol, an essential step during cardiolipin biosynthesis; null mutant is sensitive to tunicamycin, DTT
S000001143	YHR101C	BIG1	Bad In Glucose	314874	315968	-1	Integral membrane protein of the endoplasmic reticulum	Integral membrane protein of the endoplasmic reticulum; required for normal content of cell wall beta-1,6-glucan
S000001145	YHR103W	SBE22	similar to SBE2	320414	322972	1	Protein involved in bud growth	Protein involved in bud growth; involved in the transport of cell wall components from the Golgi to the cell surface; similar in structure and functionally redundant with Sbe2p; SBE22 has a paralog, SBE2, that arose from the whole genome duplication
S000001146	YHR104W	GRE3	Genes de Respuesta a Estres (stress responsive genes)	323409	324392	1	Aldose reductase	Aldose reductase; involved in methylglyoxal, d-xylose, arabinose, and galactose metabolism; stress induced (osmotic, ionic, oxidative, heat shock, starvation and heavy metals); regulated by HOG pathway; overexpression allows xylose fermentation in strains expressing heterologous xylitol dehydrogenase and xylulokinase; protein abundance increases in response to DNA replication stress
S000001147	YHR105W	YPT35	""	324766	325410	1	Endosomal protein of unknown function	Endosomal protein of unknown function; contains a phox (PX) homology domain; binds to both phosphatidylinositol-3-phosphate (PtdIns(3)P) and proteins involved in ER-Golgi or vesicular transport
S000001148	YHR106W	TRR2	ThioRedoxin Reductase	325598	326626	1	Mitochondrial thioredoxin reductase	Mitochondrial thioredoxin reductase; involved in protection against oxidative stress, required with Glr1p to maintain the redox state of Trx3p; contains active-site motif (CAVC) present in prokaryotic orthologs; binds NADPH and FAD; TRR2 has a paralog, TRR1, that arose from the whole genome duplication
S000001150	YHR108W	GGA2	Golgi-localized, Gamma-adaptin ear homology, Arf-binding protein	328303	330060	1	Protein that regulates Arf1p and Arf2p to facilitate Golgi trafficking	Protein that regulates Arf1p and Arf2p to facilitate Golgi trafficking; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; K63-specific Ub chain binding receptor with moderate selectivity; homologous to gamma-adaptin; GGA2 has a paralog, GGA1, that arose from the whole genome duplication
S000001151	YHR109W	CTM1	CyTochrome c Methyltransferase	330310	332067	1	Cytochrome c lysine methyltransferase	Cytochrome c lysine methyltransferase; trimethylates residue 72 of apo-cytochrome c (Cyc1p) in the cytosol; not required for normal respiratory growth
S000001152	YHR110W	ERP5	Emp24p/Erv25p Related Protein	332282	332920	1	Protein with similarity to Emp24p and Erv25p	Protein with similarity to Emp24p and Erv25p; member of the p24 family involved in ER to Golgi transport
S000001153	YHR111W	UBA4	UBiquitin-Activating	333072	334394	1	E1-like protein that activates Urm1p before urmylation	E1-like protein that activates Urm1p before urmylation; also acts in thiolation of the wobble base of cytoplasmic tRNAs by adenylating and then thiolating Urm1p; receives sulfur from Tum1p
S000001154	YHR112C	""	""	334527	335663	-1	Protein of unknown function	Protein of unknown function; localizes to the cytoplasm and nucleus; overexpression affects protein trafficking through the endocytic pathway
S000001155	YHR113W	APE4	""	336337	337809	1	Cytoplasmic aspartyl aminopeptidase with possible vacuole function	Cytoplasmic aspartyl aminopeptidase with possible vacuole function; targeted to vacuole via Vps10p-dependent endosomal vacuolar protein sorting pathway and via CVT pathway; cleaves unblocked N-terminal acidic amino acids from peptide substrates; forms 12-subunit homo-oligomer; M18 metalloprotease family
S000001156	YHR114W	BZZ1	""	338083	339984	1	SH3 domain protein implicated in regulating actin polymerization	SH3 domain protein implicated in regulating actin polymerization; able to recruit actin polymerization machinery through its SH3 domains; colocalizes with cortical actin patches and Las17p; interacts with type I myosins
S000001157	YHR115C	DMA1	Defective in Mitotic Arrest	340109	341359	-1	Ubiquitin-protein ligase (E3)	Ubiquitin-protein ligase (E3); controls septin dynamics, spindle position checkpoint (SPOC) with ligase Dma2p by regulating recruitment of Elm1p to bud neck; regulates levels of eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ubiquitinates cyclin Pcl1p; ortholog of human RNF8, similar to human Chfr; contains FHA, RING fingers; DMA1 has a paralog, DMA2, that arose from the whole genome duplication
S000001158	YHR116W	COX23	Cytochrome OXidase	341665	342120	1	Protein that functions in mitochondrial copper homeostasis	Protein that functions in mitochondrial copper homeostasis; mitochondrial intermembrane space protein; essential for functional cytochrome oxidase expression; homologous to Cox17p; contains twin cysteine-x9-cysteine motifs
S000001159	YHR117W	TOM71	Translocase of the Outer Mitochondrial membrane	342349	344268	1	Mitochondrial outer membrane protein	Mitochondrial outer membrane protein; probable minor component of the TOM (translocase of outer membrane) complex responsible for recognition and import of mitochondrially directed proteins; TOM71 has a paralog, TOM70, that arose from the whole genome duplication
S000001160	YHR118C	ORC6	Origin Recognition Complex	344321	345628	-1	Subunit of the origin recognition complex (ORC)	Subunit of the origin recognition complex (ORC); ORC directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; phosphorylated by Cdc28p; mutation in the human Orc6p is linked to Meier-Gorlin syndrome
S000001162	YHR120W	MSH1	MutS Homolog	349574	352453	1	DNA-binding protein of the mitochondria	DNA-binding protein of the mitochondria; involved in repair of mitochondrial DNA; has ATPase activity and binds to DNA mismatches; has homology to E. coli MutS; transcription is induced during meiosis
S000001163	YHR121W	LSM12	Like SM	352756	353319	1	Protein of unknown function that may function in RNA processing	Protein of unknown function that may function in RNA processing; interacts with Pbp1p and Pbp4p and associates with ribosomes; contains an RNA-binding LSM domain and an AD domain; GFP-fusion protein is induced by the DNA-damaging agent MMS; relative distribution to the nucleus increases upon DNA replication stress
S000001165	YHR123W	EPT1	EthanolaminePhosphoTransferase	354815	356081	1	sn-1,2-diacylglycerol ethanolamine- and cholinephosphotranferase	sn-1,2-diacylglycerol ethanolamine- and cholinephosphotranferase; not essential for viability; EPT1 has a paralog, CPT1, that arose from the whole genome duplication
S000001166	YHR124W	NDT80	Non-DiTyrosine	356561	358444	1	Meiosis-specific transcription factor	Meiosis-specific transcription factor; required for exit from pachytene and for full meiotic recombination; activates middle sporulation genes; competes with Sum1p for binding to promoters containing middle sporulation elements (MSE) 
S000001167	YHR125W	""	""	358858	359163	1	Putative protein of unknown function	Putative protein of unknown function; conserved across S. cerevisiae strains
S000001168	YHR126C	ANS1	""	359702	360181	-1	Putative GPI protein	Putative GPI protein; SWAT-GFP and mCherry fusion proteins localize to the vacuole; transcription dependent upon Azf1p
S000001169	YHR127W	""	""	360913	361644	1	Protein of unknown function	Protein of unknown function; localizes to the nucleus; required for asymmetric localization of Kar9p during mitosis
S000001171	YHR129C	ARP1	Actin-Related Protein	362999	364153	-1	Actin-related protein of the dynactin complex	Actin-related protein of the dynactin complex; required for spindle orientation and nuclear migration; forms actin-like short filament composed of 9 or 10 Arp1p monomers; putative ortholog of mammalian centractin
S000001172	YHR130C	""	""	364965	365300	-1	Putative protein of unknown function	Putative protein of unknown function; conserved among S. cerevisiae strains; YHR130C is not an essential gene
S000001173	YHR131C	""	""	365340	367892	-1	Putative protein of unknown function	Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; overexpression causes cell cycle delay or arrest; contains a PH domain and binds phosphatidylinositols and other lipids in a large-scale study; YHR131C has a paralog, YNL144C, that arose from the whole genome duplication
S000001174	YHR132C	ECM14	ExtraCellular Mutant	368502	369794	-1	Conserved fungal protein with similarity to zinc carboxypeptidases	Conserved fungal protein with similarity to zinc carboxypeptidases; in some strains required for normal cell wall assembly
S000001175	YHR133C	NSG1	""	370722	371597	-1	Protein involved in regulation of sterol biosynthesis	Protein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyze the rate-limiting step in sterol biosynthesis; forms foci at the nuclear periphery upon DNA replication stress; relocalizes to the cytosol in response to hypoxia; homolog of mammalian INSIG proteins; NSG1 has a paralog, NSG2, that arose from the whole genome duplication
S000001176	YHR134W	WSS1	Weak Suppressor of Smt3	371749	372558	1	SUMO-ligase and SUMO-targeted metalloprotease	SUMO-ligase and SUMO-targeted metalloprotease; involved in DNA repair; removes DNA-protein crosslinks at stalled replication forks during replication of damaged DNA; clears chromatin-bound sumoylated proteins; localizes to single spot on nuclear periphery of mother cells but not daughters; exhibits vacuolar localization upon genotoxic stress; activated by DNA binding; member of minigluzincins protease family with mammalian DVC1/Spartan
S000001177	YHR135C	YCK1	Yeast Casein Kinase I homolog	372694	374310	-1	Palmitoylated plasma membrane-bound casein kinase I (CK1) isoform	Palmitoylated plasma membrane-bound casein kinase I (CK1) isoform; shares redundant functions with Yck2p in morphogenesis, proper septin assembly, endocytic trafficking, and glucose sensing; stabilized by Sod1p binding in the presence of glucose and oxygen, causing glucose repression of respiratory metabolism; involved in the phosphorylation and regulation of glucose sensor Rgt2p; YCK1 has a paralog, YCK2, that arose from the whole genome duplication
S000001178	YHR136C	SPL2	Suppressor of PLc1 deletion	374654	375100	-1	Protein with similarity to cyclin-dependent kinase inhibitors	Protein with similarity to cyclin-dependent kinase inhibitors; downregulates low-affinity phosphate transport during phosphate limitation by targeting Pho87p to the vacuole; upstream region harbors putative hypoxia response element (HRE) cluster; overproduction suppresses a plc1 null mutation; promoter shows an increase in Snf2p occupancy after heat shock; GFP-fusion protein localizes to the cytoplasm
S000001179	YHR137W	ARO9	AROmatic amino acid requiring	375709	377250	1	Aromatic aminotransferase II	Aromatic aminotransferase II; involved in tyrosine, phenylalanine, and methionine metabolism; involved with Aro8p in production of kynurenic acid to detoxify excess tryptophan
S000001180	YHR138C	""	""	377355	377699	-1	Protein of unknown function	Protein of unknown function; similar to Pbi2p; double null mutant lacking Pbi2p and Yhr138cp exhibits highly fragmented vacuoles; protein abundance increases in response to DNA replication stress
S000001181	YHR139C	SPS100	SPorulation Specific	378219	379199	-1	Protein required for spore wall maturation	Protein required for spore wall maturation; expressed during sporulation; may be a component of the spore wall; expression also induced in cells treated with the mycotoxin patulin; SPS100 has a paralog, YGP1, that arose from the whole genome duplication
S000001182	YHR140W	""	""	380572	381291	1	Endoplasmic reticulum protein	Endoplasmic reticulum protein; may contribute to maintenance of ER and nuclear morphology; null mutant shows discontinuity in nuclear envelope, clustering of nuclear pores, and lacks cortical ER
S000001183	YHR141C	RPL42B	Ribosomal Protein of the Large subunit	381990	382751	-1	Ribosomal 60S subunit protein L42B	Ribosomal 60S subunit protein L42B; required for propagation of the killer toxin-encoding M1 double-stranded RNA satellite of the L-A double-stranded RNA virus; homologous to mammalian ribosomal protein L36A, no bacterial homolog; RPL42B has a paralog, RPL42A, that arose from the whole genome duplication
S000001184	YHR142W	CHS7	CHitin Synthase-related	383538	384488	1	Protein of unknown function	Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress
S000001186	YHR143W	DSE2	Daughter Specific Expression	385510	386487	1	Daughter cell-specific secreted protein with similarity to glucanases	Daughter cell-specific secreted protein with similarity to glucanases; degrades cell wall from the daughter side causing daughter to separate from mother; localizes in birth scars; expression is repressed by cAMP
S000001189	YHR146W	CRP1	Cruciform DNA-Recognizing Protein	390300	391697	1	Protein that binds to cruciform DNA structures	Protein that binds to cruciform DNA structures; CRP1 has a paralog, MDG1, that arose from the whole genome duplication
S000001190	YHR147C	MRPL6	Mitochondrial Ribosomal Protein, Large subunit	392639	393283	-1	Mitochondrial ribosomal protein of the large subunit	Mitochondrial ribosomal protein of the large subunit
S000001192	YHR149C	SKG6	Suppressor of lethality of Kex2 Gas1 double null mutant	394455	396659	-1	Integral membrane protein	Integral membrane protein; localizes primarily to growing sites such as the bud tip or the cell periphery; potential Cdc28p substrate; Skg6p interacts with Zds1p and Zds2p; SKG6 has a paralog, TOS2, that arose from the whole genome duplication
S000001193	YHR150W	PEX28	PEroXisome related	397251	398990	1	Peroxisomal integral membrane peroxin	Peroxisomal integral membrane peroxin; involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p
S000001194	YHR151C	MTC6	Maintenance of Telomere Capping	399268	400848	-1	Protein of unknown function	Protein of unknown function; mtc6 is synthetically sick with cdc13-1; SWAT-GFP and mCherry fusion proteins localize to the vacuole while SWAT-GFP fusion also localizes to the endoplasmic reticulum
S000001195	YHR152W	SPO12	SPOrulation	401434	401955	1	Nucleolar protein of unknown function	Nucleolar protein of unknown function; positive regulator of mitotic exit; involved in regulating release of Cdc14p from the nucleolus in early anaphase, may play similar role in meiosis; SPO12 has a paralog, BNS1, that arose from the whole genome duplication
S000001196	YHR153C	SPO16	SPOrulation	402086	402682	-1	Meiosis-specific protein involved in synaptonemal complex assembly	Meiosis-specific protein involved in synaptonemal complex assembly; implicated in regulation of crossover formation; required for sporulation
S000001197	YHR154W	RTT107	Regulator of Ty1 Transposition	402966	406178	1	BRCA1 C-terminal domain protein needed for recovery from DNA damage	BRCA1 C-terminal domain protein needed for recovery from DNA damage; implicated in Mms22-dependent DNA repair during S phase; involved in recruiting SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress
S000001198	YHR155W	LAM1	Lipid transfer protein Anchored at Membrane contact sites	407103	410789	1	Putative sterol transfer protein	Putative sterol transfer protein; localizes to puncta in the cortical ER; probable role in retrograde transport of sterols from the plasma membrane to the ER; one of six StART-like domain-containing proteins in yeast that may be involved in sterol transfer between intracellular membranes; conserved across eukaryotes; contains GRAM, StART-like (VASt) and two PH-like domains
S000001199	YHR156C	LIN1	LINk	411384	412406	-1	Non-essential component of U5 snRNP	Non-essential component of U5 snRNP; nuclear protein; physically interacts with Irr1p of cohesin complex; may link together proteins involved in chromosome segregation, mRNA splicing and DNA replication
S000001200	YHR157W	REC104	RECombination	412907	413455	1	Protein involved in early stages of meiotic recombination	Protein involved in early stages of meiotic recombination; required for meiotic crossing over; forms a complex with Rec102p and Spo11p necessary during the initiation of recombination
S000001201	YHR158C	KEL1	KELch repeat	413685	417179	-1	Protein required for proper cell fusion and cell morphology	Protein required for proper cell fusion and cell morphology; acts as phosphorylation-regulated noise suppressor of pheromone signaling pathway; forms a complex with Bud14p and Kel2p that regulates Bnr1p (formin) to affect actin cable assembly, cytokinesis, and polarized growth; functions in a complex with Kel2p to negatively regulate mitotic exit, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate
S000001202	YHR159W	TDA11	Topoisomerase I Damage Affected	417549	419063	1	Putative protein of unknown function	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; potential Cdc28p substrate; null mutant is sensitive to expression of the top1-T722A allele
S000001203	YHR160C	PEX18	PEroXin	419221	420072	-1	Peroxin	Peroxin; required for targeting of peroxisomal matrix proteins containing PTS2; interacts with Pex7p; partially redundant with Pex21p; primarily responsible for peroxisomal import during growth on oleate, and expression is induced during oleate growth
S000001204	YHR161C	YAP1801	Yeast Assembly Polypeptide	420373	422286	-1	Protein of the AP180 family, involved in clathrin cage assembly	Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1801 has a paralog, YAP1802, that arose from the whole genome duplication
S000001205	YHR162W	MPC2	Mitochondrial Pyruvate Carrier	423072	423461	1	Highly conserved subunit of the mitochondrial pyruvate carrier (MPC)	Highly conserved subunit of the mitochondrial pyruvate carrier (MPC); expressed during growth on fermentable carbon sources, and heterodimerizes with Mpc1p to form the fermentative isoform of MPC; MPC localizes to the mitochondrial inner membrane and mediates pyruvate uptake; MPC2 paralog, MPC3, heterodimerizes with Mpc1p to form the respiratory MPC isoform
S000001206	YHR163W	SOL3	Suppressor Of Los1-1	423724	424473	1	6-phosphogluconolactonase	6-phosphogluconolactonase; catalyzes the second step of the pentose phosphate pathway; weak multicopy suppressor of los1-1 mutation; homologous to Sol2p and Sol1p; SOL3 has a paralog, SOL4, that arose from the whole genome duplication
S000001207	YHR164C	DNA2	DNA synthesis defective	424610	429178	-1	ATP-dependent 5'-flap endonuclease and DNA helicase	ATP-dependent 5'-flap endonuclease and DNA helicase; tracking protein for flap cleavage during Okazaki fragment maturation; involved in DNA repair/processing of meiotic DNA double strand breaks; cell cycle-dependent localization to telomeric chromatin and replicating chromosomes; required for telomerase-dependent telomere synthesis; stability, localization and nuclease activity regulated by sumoylation; forms nuclear foci upon replication stress; human homolog DNA2 complements yeast dna2 mutant
S000001209	YHR166C	CDC23	Cell Division Cycle	437170	439050	-1	Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C)	Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
S000001210	YHR167W	THP2	THO2 - HPR1 Phenotype	439342	440127	1	Subunit of the THO and TREX complexes	Subunit of the THO and TREX complexes; THO connects transcription elongation and mitotic recombination, and TREX is recruited to activated genes and couples transcription to mRNA export; involved in telomere maintenance
S000001211	YHR168W	MTG2	MiTochondrial Gtpase	440377	441933	1	Putative GTPase	Putative GTPase; member of the Obg family; peripheral protein of the mitochondrial inner membrane that associates with the large ribosomal subunit; required for mitochondrial translation, possibly via a role in ribosome assembly
S000001213	YHR170W	NMD3	Nonsense-Mediated mRNA Decay	443828	445384	1	Protein involved in nuclear export of the large ribosomal subunit	Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
S000001214	YHR171W	ATG7	AuTophaGy related	445713	447605	1	Autophagy-related protein and dual specificity member of the E1 family	Autophagy-related protein and dual specificity member of the E1 family; mediates the attachment of Atg12p to Atg5p and Atg8p to phosphatidylethanolamine which are required steps in autophagosome formation; E1 enzymes are also known as ubiquitin-activating enzymes; involved in methionine restriction extension of chronological lifespan in an autophagy-dependent manner
S000001219	YHR176W	FMO1	Flavin containing MonoOxygenase	454229	455527	1	Flavin-containing monooxygenase	Flavin-containing monooxygenase; localized to the cytoplasmic face of the ER membrane; catalyzes oxidation of biological thiols to maintain the ER redox buffer ratio for correct folding of disulfide-bonded proteins
S000001220	YHR177W	ROF1	Regulator Of Fluffy	456591	457952	1	Putative transcription factor containing a WOPR domain	Putative transcription factor containing a WOPR domain; binds DNA in vitro; similar to C. albicans Wor1p transcription factor that regulates white-opaque switching; overexpression causes a cell cycle delay or arrest
S000001221	YHR178W	STB5	Sin Three Binding protein	459299	461530	1	Transcription factor	Transcription factor; regulates multidrug resistance and the oxidative stress response; forms a heterodimer with Pdr1p; contains a Zn(II)2Cys6 zinc finger domain that interacts with a pleiotropic drug resistance element in vitro; negative regulator of autophagy, modulating the transcription of genes involved in NADPH production and the pentose phosphate pathway; Stb5p expression is reduced during nitrogen starvation and autophagy induction, while STB5 mRNA levels are significantly increased
S000001222	YHR179W	OYE2	Old Yellow Enzyme	462502	463704	1	Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN)	Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN); responsible for geraniol reduction into citronellol during fermentation; homologous to Oye3p with different ligand binding and catalytic properties; may be involved in sterol metabolism, oxidative stress response, and programmed cell death; protein abundance increases in response to DNA replication stress
S000001223	YHR180W	""	""	465178	465669	1	Putative protein of unknown function	Putative protein of unknown function; conserved among S. cerevisiae strains
S000001224	YHR181W	SVP26	Sed5 Vesicle Protein	467228	467914	1	ER exit adaptor for Golgi mannosyltransferases	ER exit adaptor for Golgi mannosyltransferases; facilitates the exit of Kre2p, Ktr1p and Mnn4p from the ER; involved in COPII-coated vesicle cargo loading and transport; may also promote the retention of proteins in the early Golgi compartment; integral membrane protein of early Golgi and ER
S000001225	YHR182W	RGD3	""	468219	470576	1	GTPase activating protein (GAP) for Rho3p, involved in cell polarity g	GTPase activating protein (GAP) for Rho3p, involved in cell polarity green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and cytoplasm; relocalizes from bud neck to cytoplasm upon DNA replication stress
S000001226	YHR183W	GND1	6-phosphoGlucoNateDehydrogenase	470960	472429	1	6-phosphogluconate dehydrogenase (decarboxylating)	6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone and adaptation to oxidative stress; GND1 has a paralog, GND2, that arose from the whole genome duplication
S000001227	YHR184W	SSP1	""	472744	474459	1	Protein involved in the control of meiotic nuclear division	Protein involved in the control of meiotic nuclear division; involved in the coordination of meiosis with spore formation; subunit of the leading edge protein (LEP) complex (Ssp1-Ady3-Don1-Irc10) that forms a ring-like structure at the leading edge of the prospore membrane during meiosis II; required for assembly of the leading edge coat and both prospore membrane shaping and organization; transcription is induced midway through meiosis
S000001228	YHR185C	PFS1	Prospore Formation at Spindles	474627	475340	-1	Sporulation protein required for prospore membrane formation	Sporulation protein required for prospore membrane formation; required for prospore membrane formation at selected spindle poles; ensures functionality of all four spindle pole bodies during meiosis II; not required for meiotic recombination or meiotic chromosome segregation
S000001229	YHR186C	KOG1	Kontroller Of Growth	475999	480672	-1	Ubiquitin-binding protein	Ubiquitin-binding protein; centrally regulates metabolic homeostasis and carbon utilization during nutrient limitation by managing Snf1p activity; subunit of TORC1 rapamycin-sensitive complex involved in growth control; central scaffold that holds the different components of TORC1 together; contains four HEAT repeats and seven WD-40 repeats
S000001230	YHR187W	IKI1	Insensitive to KIller toxin	480990	481919	1	Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex	Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; iki1 mutations confer resistance to the K. lactis toxin zymocin; localizes to cytosol, nucleus and peroxisomes
S000001232	YHR189W	PTH1	Peptidyl-Trna Hydrolase	484028	484600	1	One of two mitochondrially-localized peptidyl-tRNA hydrolases	One of two mitochondrially-localized peptidyl-tRNA hydrolases; dispensable for respiratory growth on rich medium, but required for respiratory growth on minimal medium; see also PTH2
S000001234	YHR191C	CTF8	Chromosome Transmission Fidelity	486230	486631	-1	Subunit of a complex with Ctf18p	Subunit of a complex with Ctf18p; shares some subunits with Replication Factor C; required for sister chromatid cohesion
S000001235	YHR192W	LNP1	LuNaPark family member	486826	487662	1	Lunapark family member involved in ER network formation	Lunapark family member involved in ER network formation; regulates the ER asymmetry-induced inheritance block during ER stress; localizes to ER junctions and this localization is regulated by the yeast atlastin ortholog Sey1p; required for reticulophagy where it is proposed to stabilize the actin-dependent remodeling of the ER; interacts with the reticulon protein Rtn1p; induced in response to the DNA-damaging agent MMS
S000001236	YHR193C	EGD2	Enhancer of Gal4 DNA binding	487712	488236	-1	Alpha subunit of the nascent polypeptide-associated complex (NAC)	Alpha subunit of the nascent polypeptide-associated complex (NAC); involved in protein sorting and translocation; associated with cytoplasmic ribosomes
S000001237	YHR194W	MDM31	Mitochondrial Distribution and Morphology	488657	490396	1	Mitochondrial protein that may have a role in phospholipid metabolism	Mitochondrial protein that may have a role in phospholipid metabolism; inner membrane protein with similarity to Mdm32p; required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MMM2, MDM10, MDM12, and MDM34
S000001238	YHR195W	NVJ1	Nucleus-Vacuole Junction	490747	491712	1	Nuclear envelope protein	Nuclear envelope protein; anchored to nuclear inner membrane, interacts with vacuolar membrane protein Vac8p to promote formation of nucleus-vacuole junctions during piecemeal microautophagy of the nucleus (PMN); targeted to vacuole via AP-3 pathway
S000001239	YHR196W	UTP9	U Three Protein	491931	493658	1	Nucleolar protein	Nucleolar protein; component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
S000001240	YHR197W	RIX1	RIbosome eXport	493896	496187	1	Component of the Rix1 complex and possibly pre-replicative complexes	Component of the Rix1 complex and possibly pre-replicative complexes; required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-replicative complex (pre-RC) formation and maintenance during DNA replication licensing; relocalizes to the cytosol in response to hypoxia; essential gene
S000001241	YHR198C	AIM18	Altered Inheritance rate of Mitochondria	496315	497280	-1	Hemoprotein	Hemoprotein; protein of unknown function that localizes to the mitochondrial inner mnembrane; null mutant displays elevated frequency of mitochondrial genome loss; contains a chalcone isomerase (CHI) domain, a predicted N-terminal transmembrane domain and an N-terminal mitochondrial targeting sequence; lacks CHI catalytic activity
S000001242	YHR199C	AIM46	Altered Inheritance rate of Mitochondria	497490	498422	-1	Hemoprotein	Hemoprotein; protein of unknown function that localizes to the mitochondrial inner mnembrane; null mutant displays elevated frequency of mitochondrial genome loss; contains a chalcone isomerase (CHI) domain, a predicted N-terminal transmembrane domain and an N-terminal mitochondrial targeting sequence; lacks CHI catalytic activity
S000001243	YHR200W	RPN10	Regulatory Particle Non-ATPase	499079	499885	1	Proteasome polyubiquitin receptor	Proteasome polyubiquitin receptor; non-ATPase subunit of the 19S regulatory particle (RP) of the 26S proteasome that links the RP base to the lid; N-terminus plays a role in the assembly of the regulatory particle (RP); ubiquitin-interacting motif selectively binds to polyubiquitin chains; homolog of the mammalian S5a protein
S000001245	YHR202W	SMN1	Scavenger MonoNucleotidase	502388	504196	1	Putative AMP hydrolase	Putative AMP hydrolase; secreted protein proposed to function as an adenosine monophosphatase in NAD+ degradation, via nucleoside salvage pathway that converts NAD+ to AMP and adenosine; overexpression results in adenosine accumulation and reduced levels of NAD+, deletion results in increased NAD+ and NADH; targeted to vacuole via Vps10p-dependent endosomal vacuolar protein sorting pathway; similar to human NT5E/CD73 gene involved in cancer progression
S000001246	YHR203C	RPS4B	Ribosomal Protein of the Small subunit	504476	505530	-1	Protein component of the small (40S) ribosomal subunit	Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4B has a paralog, RPS4A, that arose from the whole genome duplication
S000001247	YHR204W	MNL1	MaNnosidase-Like protein	506319	508709	1	Alpha-1,2-specific exomannosidase of the endoplasmic reticulum	Alpha-1,2-specific exomannosidase of the endoplasmic reticulum; involved in glycan trimming of both folded and misfolded glycoproteins; complexes with Pdi1p, and trims a mannose from Man8GlcNac2 glycans to generate Man7GlcNac2, an oligosaccharide signal on glycoproteins destined for ER-associated protein degradation; requires Pdi1p for stability and substrate recognition; human homolog EDEM1 can complement yeast null mutant
S000001248	YHR205W	SCH9	""	509363	511837	1	AGC family protein kinase	AGC family protein kinase; functional ortholog of mammalian S6 kinase; phosphorylated by Tor1p and required for TORC1-mediated regulation of ribosome biogenesis, translation initiation, and entry into G0 phase; involved in transactivation of osmostress-responsive genes; regulates G1 progression, cAPK activity and nitrogen activation of the FGM pathway; integrates nutrient signals and stress signals from sphingolipids to regulate lifespan
S000001249	YHR206W	SKN7	Suppressor of Kre Null	512732	514600	1	Nuclear response regulator and transcription factor	Nuclear response regulator and transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; part of a branched two-component signaling system; required for optimal induction of heat-shock genes in response to oxidative stress; involved in osmoregulation; relocalizes to the cytosol in response to hypoxia; SKN7 has a paralog, HMS2, that arose from the whole genome duplication
S000001250	YHR207C	SET5	SET domain-containing	514905	516485	-1	Methyltransferase involved in methylation of histone H4 Lys5, -8, -12	Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus
S000001252	YHR209W	CRG1	Cantharidin Resistance Gene	519437	520312	1	S-AdoMet-dependent methyltransferase involved in lipid homeostasis	S-AdoMet-dependent methyltransferase involved in lipid homeostasis; mediates resistance to a drug cantharidin
S000001253	YHR210C	""	""	520712	521737	-1	Putative aldose 1-epimerase superfamily protein	Putative aldose 1-epimerase superfamily protein; non-essential gene; highly expressed under anaeorbic conditions
S000001263	YIL001W	""	""	353940	355481	1	Putative protein of unknown function	Putative protein of unknown function; contains a BTB/POZ domain which generally function in protein interactions; deletion slightly improved competitive fitness in rich media; GFP-tagged protein is localized to the cytoplasm
S000001264	YIL002C	INP51	INositol polyphosphate 5-Phosphatase	350591	353431	-1	Phosphatidylinositol 4,5-bisphosphate 5-phosphatase	Phosphatidylinositol 4,5-bisphosphate 5-phosphatase; synaptojanin-like protein with an N-terminal Sac1 domain, plays a role in phosphatidylinositol 4,5-bisphosphate homeostasis and in endocytosis; null mutation confers cold-tolerant growth
S000001265	YIL003W	CFD1	Cytosolic Fe-S cluster Deficient	349122	350003	1	Highly conserved iron-sulfur cluster binding protein	Highly conserved iron-sulfur cluster binding protein; localized in the cytoplasm; forms a complex with Nbp35p that is involved in iron-sulfur protein assembly in the cytosol
S000001267	YIL005W	EPS1	ER-retained Pma1 Suppressing	345692	347797	1	ER protein with chaperone and co-chaperone activity	ER protein with chaperone and co-chaperone activity; involved in retention of resident ER proteins; has a role in recognizing proteins targeted for ER-associated degradation (ERAD), member of the protein disulfide isomerase family
S000001268	YIL006W	YIA6	""	344062	345183	1	Mitochondrial NAD+ transporter	Mitochondrial NAD+ transporter; involved in the transport of NAD+ into the mitochondria (see also YEA6); member of the mitochondrial carrier subfamily; disputed role as a pyruvate transporter; has putative mouse and human orthologs; YIA6 has a paralog, YEA6, that arose from the whole genome duplication; human NAD+ transporter MCART1/SLC25A51 functionally complements the yia6 yea6 double null mutant, and yeast YIA6 reciprocally complements defects in MCART1/SLC25A51 null cells
S000001269	YIL007C	NAS2	Non-ATPase Subunit	342994	343656	-1	Evolutionarily conserved 19S regulatory particle assembly-chaperone	Evolutionarily conserved 19S regulatory particle assembly-chaperone; involved in assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); non-essential gene; interacts with Rpn4p; protein abundance increases in response to DNA replication stress; ortholog of human p27
S000001270	YIL008W	URM1	Ubiquitin Related Modifier	342536	342835	1	Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs	Ubiquitin-like protein involved in thiolation of cytoplasmic tRNAs; receives sulfur from the E1-like enzyme Uba4p and transfers it to tRNA; also functions as a protein tag with roles in nutrient sensing and oxidative stress response
S000001271	YIL009W	FAA3	Fatty Acid Activation	339344	341428	1	Long chain fatty acyl-CoA synthetase	Long chain fatty acyl-CoA synthetase; activates imported fatty acids with a preference for C16:0-C18:0 chain lengths; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
S000001272	YIL010W	DOT5	Disruptor Of Telomeric silencing	334882	335529	1	Nuclear thiol peroxidase	Nuclear thiol peroxidase; functions as an alkyl-hydroperoxide reductase during post-diauxic growth
S000001273	YIL011W	TIR3	TIp1-Related	333727	334536	1	Cell wall mannoprotein	Cell wall mannoprotein; member of Srp1p/Tip1p family of serine-alanine-rich proteins; expressed under anaerobic conditions and required for anaerobic growth; TIR3 has a paralog, TIR2, that arose from the whole genome duplication
S000001274	YIL012W	""	""	333011	333352	1	Putative protein of unknown function	Putative protein of unknown function; conserved across S. cerevisiae strains
S000001275	YIL013C	PDR11	Pleiotropic Drug Resistance	328207	332442	-1	ATP-binding cassette (ABC) transporter	ATP-binding cassette (ABC) transporter; multidrug transporter involved in multiple drug resistance; mediates sterol uptake when sterol biosynthesis is compromised; regulated by Pdr1p; required for anaerobic growth; PDR11 has a paralog, AUS1, that arose from the whole genome duplication
S000001276	YIL014W	MNT3	MaNnosylTransferase	326103	327995	1	Alpha-1,3-mannosyltransferase	Alpha-1,3-mannosyltransferase; adds the fourth and fifth alpha-1,3-linked mannose residues to O-linked glycans during protein O-glycosylation
S000001277	YIL015W	BAR1	BARrier to the alpha factor response	322342	324105	1	Aspartyl protease secreted to periplasmic space of mating type a cell	Aspartyl protease secreted to periplasmic space of mating type a cell; helps cells monitor availability of mating partners; cleaves and inactivates alpha factor allowing cells to recover from alpha-factor-induced cell cycle arrest
S000001278	YIL016W	SNL1	Suppressor of Nup116-C Lethal	321454	321933	1	Ribosome-associated protein	Ribosome-associated protein; proposed to act in protein synthesis, nuclear pore complex biogenesis and maintenance as well as protein folding and prion maintenance; ; has similarity to the mammalian BAG-1 protein
S000001279	YIL017C	VID28	Vacuolar Import and Degradation	318200	320965	-1	GID Complex subunit, serves as adaptor for regulatory subunit Vid24p	GID Complex subunit, serves as adaptor for regulatory subunit Vid24p; protein involved in proteasome-dependent catabolite degradation of fructose-1,6-bisphosphatase (FBPase); localized to the nucleus and the cytoplasm
S000001280	YIL018W	RPL2B	Ribosomal Protein of the Large subunit	316768	317932	1	Ribosomal 60S subunit protein L2B	Ribosomal 60S subunit protein L2B; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2B has a paralog, RPL2A, that arose from the whole genome duplication; expression is upregulated at low temperatures
S000001281	YIL019W	FAF1	Forty (40) S Assembly Factor 	315093	316133	1	Protein required for pre-rRNA processing	Protein required for pre-rRNA processing; also required for 40S ribosomal subunit assembly
S000001282	YIL020C	HIS6	HIStidine requiring	314035	314820	-1	Enzyme that catalyzes the fourth step in the histidine pathway	Enzyme that catalyzes the fourth step in the histidine pathway; Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts
S000001283	YIL021W	RPB3	RNA Polymerase B	312905	313861	1	RNA polymerase II third largest subunit B44	RNA polymerase II third largest subunit B44; part of central core; similar to prokaryotic alpha subunit
S000001284	YIL022W	TIM44	Translocase of the Inner Mitochondrial membrane	311165	312460	1	Essential component of the TIM23 complex	Essential component of the TIM23 complex; tethers the import motor and regulatory factors (PAM complex) to the translocation channel (Tim23p-Tim17p core complex); TIM23 complex is short for the translocase of the inner mitochondrial membrane
S000001285	YIL023C	YKE4	Yeast ortholog of mouse KE4	309386	310426	-1	Zinc transporter	Zinc transporter; localizes to the ER; null mutant is sensitive to calcofluor white, leads to zinc accumulation in cytosol; ortholog of the mouse KE4 and member of the ZIP (ZRT, IRT-like Protein) family
S000001286	YIL024C	""	""	308602	309171	-1	Putative protein of unknown function	Putative protein of unknown function; non-essential gene; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p
S000001287	YIL025C	""	""	308201	308575	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000001288	YIL026C	IRR1	IRRegular cell behavior	304477	307929	-1	Subunit of the cohesin complex	Subunit of the cohesin complex; which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms; relocalizes to the cytosol in response to hypoxia; essential for viability
S000001289	YIL027C	EMC5	ER Membrane protein Complex	303679	304104	-1	Member of conserved ER transmembrane complex	Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response, and also shows K1 killer toxin resistance; homologous to worm B0334.15/EMC-5, fly CG15168, human MMGT
S000001290	YIL028W	""	""	302100	302498	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000001291	YIL029C	EMA17	Efficient Mitochondria targeting-Associated protein	300829	301257	-1	Protein involved in targeting mitochondrial membrane protein precursor	Protein involved in targeting mitochondrial membrane protein precursors to mitochondrial translocation system; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YIL029C has a paralog, YPR071W, that arose from a single-locus duplication
S000001292	YIL030C	SSM4	Suppressor of mrna Stability Mutant	296050	300009	-1	Membrane-embedded ubiquitin-protein ligase and retrotranslocase	Membrane-embedded ubiquitin-protein ligase and retrotranslocase; ER and inner nuclear membrane localized RING-CH domain E3 ligase involved in ER-associated protein degradation (ERAD); aids Cdc48p in the extraction of faulty membrane proteins; targets misfolded cytosolic/nucleoplasmic domains of soluble and membrane embedded proteins (ERAD-C) and Sbh2p, a transmembrane domain-containing substrate (ERAD-M); C-terminal element, conserved in human ortholog MARCH6, determines substrate selectivity
S000001293	YIL031W	ULP2	UbL-specific Protease	292633	295737	1	Peptidase that deconjugates Smt3/SUMO-1 peptides from proteins	Peptidase that deconjugates Smt3/SUMO-1 peptides from proteins; plays a role in chromosome cohesion at centromeric regions and recovery from checkpoint arrest induced by DNA damage or DNA replication defects; potential Cdc28p substrate; human homolog PML implicated in promyelocytic leukemia can partially complement yeast null mutant
S000001294	YIL032C	""	""	291961	292317	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000001296	YIL034C	CAP2	CAPping	289226	290089	-1	Beta subunit of the capping protein heterodimer (Cap1p and Cap2p)	Beta subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches and the cytokinetic contractile ring; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress
S000001297	YIL035C	CKA1	Casein Kinase Alpha subunit	287790	288908	-1	Alpha catalytic subunit of casein kinase 2 (CK2)	Alpha catalytic subunit of casein kinase 2 (CK2); a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; regulates Fkh1p-mediated donor preference during mating-type switching
S000001298	YIL036W	CST6	Chromosome STability	285666	287429	1	Basic leucine zipper (bZIP) transcription factor from ATF/CREB family	Basic leucine zipper (bZIP) transcription factor from ATF/CREB family involved in stress-responsive regulatory network; mediates transcriptional activation of NCE103 in response to low CO2 levels; proposed to be a regulator of oleate responsive genes; involved in utilization of non-optimal carbon sources and chromosome stability; relocalizes to the cytosol in response to hypoxia; CST6 has a paralog, ACA1, that arose from the whole genome duplication
S000001299	YIL037C	PRM2	Pheromone-Regulated Membrane protein	283029	284999	-1	Pheromone-regulated protein	Pheromone-regulated protein; predicted to have 4 transmembrane segments and a coiled coil domain; regulated by Ste12p; required for efficient nuclear fusion
S000001300	YIL038C	NOT3	Negative On TATA	280142	282652	-1	Component of the CCR4-NOT core complex, involved in mRNA decapping	Component of the CCR4-NOT core complex, involved in mRNA decapping; involved in transcription initiation and elongation and in mRNA degradation; conserved lysine in human homolog of Not3p and Not5p is mutated in cancers
S000001301	YIL039W	TED1	Trafficking of Emp24p/Erv25p-dependent cargo Disrupted	278426	279847	1	GPI-glycan remodelase	GPI-glycan remodelase; conserved phosphoesterase domain-containing protein; acts together with Emp24p/Erv25p in cargo exit from the ER; functional ortholog of mammalian GPI-glycan remodelase PGAP5; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)
S000001302	YIL040W	APQ12	APical growth revealed by Quantitative morphological analysis	277723	278139	1	Nuclear envelope/ER integral membrane protein	Nuclear envelope/ER integral membrane protein; interacts and functions with Brr6p and Brl1p in lipid homeostasis; mutants are defective in nuclear pore complex biogenesis, nuclear envelope morphology, mRNA export from the nucleus and are sensitive to sterol biosynthesis inhibitors and membrane fluidizing agents; exhibits synthetic lethal genetic interactions with genes involved in lipid metabolism
S000001303	YIL041W	GVP36	Golgi Vesicle Protein	276525	277505	1	BAR domain protein that localizes to early and late Golgi vesicles	BAR domain protein that localizes to early and late Golgi vesicles; required for adaptation to varying nutrient concentrations, fluid-phase endocytosis, polarization of the actin cytoskeleton, and vacuole biogenesis
S000001304	YIL042C	PKP1	Protein Kinase of PDH	275108	276292	-1	Mitochondrial protein kinase	Mitochondrial protein kinase; involved in negative regulation of pyruvate dehydrogenase complex activity by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp2p and phosphatases Ptc5p and Ptc6p
S000001305	YIL043C	CBR1	Cytochrome b Reductase	274072	274926	-1	Cytochrome b reductase and NADH-dependent reductase for Dph3p	Cytochrome b reductase and NADH-dependent reductase for Dph3p; required for diphthamide synthesis and tRNA wobble uridine modification; also detected in mitochondria; mutation in conserved NADH binding domain of the human ortholog results in type I methemoglobinemia
S000001306	YIL044C	AGE2	ArfGAP Effector	272950	273846	-1	ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector	ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector; involved in Trans-Golgi-Network (TGN) transport; contains C2C2H2 cysteine/histidine motif
S000001307	YIL045W	PIG2	Protein Interacting with Gsy2p	271161	272777	1	Putative type-1 protein phosphatase targeting subunit	Putative type-1 protein phosphatase targeting subunit; tethers Glc7p type-1 protein phosphatase to Gsy2p glycogen synthase; PIG2 has a paralog, GIP2, that arose from the whole genome duplication
S000001308	YIL046W	MET30	METhionine requiring	268651	270573	1	F-box protein containing five copies of the WD40 motif	F-box protein containing five copies of the WD40 motif; controls cell cycle function, sulfur metabolism, and methionine biosynthesis as part of the ubiquitin ligase complex; interacts with and regulates Met4p, localizes within the nucleus; dissociation of Met30p from SCF complex in response to cadmium stress is regulated by Cdc48p
S000001309	YIL047C	SYG1	Suppressor of Yeast Gpa1	265115	267823	-1	Plasma membrane protein of unknown function	Plasma membrane protein of unknown function; targeted to vacuole via AP-3 pathway; truncation and overexpression suppresses lethality of G-alpha protein deficiency
S000001311	YIL049W	DFG10	Defective for Filamentous Growth	260158	260919	1	Probable polyprenol reductase	Probable polyprenol reductase; catalyzes conversion of polyprenol to dolichol, the precursor for N-glycosylation; involved in filamentous growth; mutations in human homolog SRD5A3 confer CDG (Congenital Disorders of Glycosylation); human SRD5A3 can complement yeast null mutant
S000001312	YIL050W	PCL7	Pho85 CycLin	258913	259770	1	Pho85p cyclin of the Pho80p subfamily	Pho85p cyclin of the Pho80p subfamily; forms a functional kinase complex with Pho85p which phosphorylates Mmr1p and is regulated by Pho81p; involved in glycogen metabolism, expression is cell-cycle regulated; PCL7 has a paralog, PCL6, that arose from the whole genome duplication
S000001313	YIL051C	MMF1	Mitochondrial Matrix Factor	257843	258280	-1	Mitochondrial protein required for transamination of isoleucine	Mitochondrial protein required for transamination of isoleucine; but not of valine or leucine; may regulate specificity of branched-chain transaminases Bat1p and Bat2p; induction of expression in response to stress is mediated by a Hog1p-regulated antisense RNA and gene looping; interacts genetically with mitochondrial ribosomal protein genes; MMF1 has a paralog, HMF1, that arose from the whole genome duplication
S000001314	YIL052C	RPL34B	Ribosomal Protein of the Large subunit	256226	257063	-1	Ribosomal 60S subunit protein L34B	Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication
S000001315	YIL053W	GPP1	Glycerol-3-Phosphate Phosphatase	255115	255867	1	Constitutively expressed DL-glycerol-3-phosphate phosphatase	Constitutively expressed DL-glycerol-3-phosphate phosphatase; also known as glycerol-1-phosphatase; involved in glycerol biosynthesis, induced in response to both anaerobic and osmotic stress; GPP1 has a paralog, GPP2, that arose from the whole genome duplication
S000001316	YIL054W	""	""	254543	254860	1	Protein of unknown function	Protein of unknown function; expressed at both mRNA and protein levels
S000001317	YIL055C	""	""	252042	253925	-1	Putative protein of unknown function	Putative protein of unknown function
S000001318	YIL056W	VHR1	VHt1 Regulator	249991	251913	1	Transcriptional activator	Transcriptional activator; required for the vitamin H-responsive element (VHRE) mediated induction of VHT1 (Vitamin H transporter) and BIO5 (biotin biosynthesis intermediate transporter) in response to low biotin concentrations; VHR1 has a paralog, VHR2, that arose from the whole genome duplication
S000001319	YIL057C	RGI2	Respiratory growth induced	247902	248396	-1	Protein of unknown function	Protein of unknown function; involved in energy metabolism under respiratory conditions; expression induced under carbon limitation and repressed under high glucose; RGI2 has a paralog, RGI1, that arose from the whole genome duplication
S000001320	YIL058W	""	""	246914	247198	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000001321	YIL059C	""	""	246550	246915	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YIL060W
S000001322	YIL060W	""	""	246392	246826	1	Mitochondrial protein of unknown function	Mitochondrial protein of unknown function; required for respiratory growth; mutant accumulates less glycogen than does wild type; null mutation results in a decrease in plasma membrane electron transport; YIL060W is not an essential gene
S000001323	YIL061C	SNP1	""	244657	245559	-1	Component of U1 snRNP required for mRNA splicing via spliceosome	Component of U1 snRNP required for mRNA splicing via spliceosome; substrate of arginine methyltransferase Hmt1p; may interact with poly(A) polymerase to regulate polyadenylation; homolog of human U1-70K, which has been linked to several types of autoimmune and neurodegenerative diseases
S000001324	YIL062C	ARC15	ARp2/3 Complex subunit	243998	244462	-1	Subunit of the ARP2/3 complex	Subunit of the ARP2/3 complex; ARP2/3 is required for the motility and integrity of cortical actin patches; has mRNA binding activity
S000001325	YIL063C	YRB2	Yeast Ran Binder	242761	243744	-1	Protein of unknown function	Protein of unknown function; involved in nuclear processes of the Ran-GTPase cycle; involved in nuclear protein export; contains Ran Binding Domain and FxFG repeats; interacts with Srm1p, GTP-Gsp1p, Rna1p and Crm1p; relocalizes to the cytosol in response to hypoxia; not essential for viability
S000001326	YIL064W	EFM4	Elongation Factor Methyltransferase	241943	242716	1	Lysine methyltransferase	Lysine methyltransferase; involved in the dimethylation of eEF1A (Tef1p/Tef2p) at lysine 316; sequence similarity to S-adenosylmethionine-dependent methyltransferases of the seven beta-strand family; role in vesicular transport
S000001327	YIL065C	FIS1	mitochondrial FISsion	241308	241775	-1	Protein involved in mitochondrial fission and peroxisome abundance	Protein involved in mitochondrial fission and peroxisome abundance; may have a distinct role in tethering protein aggregates to mitochondria in order to retain them in the mother cell; required for localization of Dnm1p and Mdv1p during mitochondrial division; mediates ethanol-induced apoptosis and ethanol-induced mitochondrial fragmentation
S000001328	YIL066C	RNR3	RiboNucleotide Reductase	238099	240708	-1	Minor isoform of large subunit of ribonucleotide-diphosphate reductase	Minor isoform of large subunit of ribonucleotide-diphosphate reductase; the RNR complex catalyzes rate-limiting step in dNTP synthesis, regulated by DNA replication and DNA damage checkpoint pathways via localization of small subunits; RNR3 has a paralog, RNR1, that arose from the whole genome duplication
S000001329	YIL067C	""	""	235724	237760	-1	Uncharacterized protein of unknown function	Uncharacterized protein of unknown function
S000001330	YIL068C	SEC6	SECretory	233057	235474	-1	Essential 88kDa subunit of the exocyst complex	Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; binds to SNARE complexes binteracting with Sec9p
S000001331	YIL069C	RPS24B	Ribosomal Protein of the Small subunit	231553	232369	-1	Protein component of the small (40S) ribosomal subunit	Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S24, no bacterial homolog; RPS24B has a paralog, RPS24A, that arose from the whole genome duplication
S000001332	YIL070C	MAM33	Mitochondrial Acidic Matrix protein	230272	231072	-1	Acidic protein of the mitochondrial matrix	Acidic protein of the mitochondrial matrix; binds unassembled large subunit proteins during ribosome assembly; subunit of a complex containing Mrx6p, Pim1p, and Pet20p that may regulate mtDNA replication; related to the human complement receptor gC1q-R 
S000001333	YIL071C	PCI8	Proteasome-COP9 signalosome (CSN)-eIF3	228660	229994	-1	Possible shared subunit of Cop9 signalosome (CSN) and eIF3	Possible shared subunit of Cop9 signalosome (CSN) and eIF3; binds eIF3b subunit Prt1p, has possible dual functions in transcriptional and translational control, contains a PCI (Proteasome-COP9 signalosome (CSN)-eIF3) domain
S000001334	YIL072W	HOP1	HOmolog Pairing	226602	228419	1	Meiosis-specific protein required for chromosome synapsis	Meiosis-specific protein required for chromosome synapsis; displays Red1p dependent localization to the unsynapsed axial-lateral elements of the synaptonemal complex; required for chiasma formation; in vitro, displays the ability to promote intra- and intermolecular synapsis between double-stranded DNA molecules and to fold DNA into rigid protein-DNA filaments
S000001335	YIL073C	SPO22	SPOrulation	222937	225954	-1	Meiosis-specific protein essential for chromosome synapsis	Meiosis-specific protein essential for chromosome synapsis; involved in completion of nuclear divisions during meiosis; induced early in meiosis
S000001336	YIL074C	SER33	SERine requiring	221081	222490	-1	3-phosphoglycerate dehydrogenase and alpha-ketoglutarate reductase	3-phosphoglycerate dehydrogenase and alpha-ketoglutarate reductase; 3PG dehydrogenase that catalyzes the first step in serine and glycine biosynthesis; also functions as an alpha-ketoglutarate reductase, converting alpha-ketoglutarate to D-2-hydroxyglutarate (D-2HG); localizes to the cytoplasm; SER33 has a paralog, SER3, that arose from the whole genome duplication
S000001337	YIL075C	RPN2	Regulatory Particle Non-ATPase	217863	220700	-1	Subunit of the 26S proteasome	Subunit of the 26S proteasome; substrate of the N-acetyltransferase Nat1p; protein abundance increases in response to DNA replication stress
S000001338	YIL076W	SEC28	SECretory	216658	217548	1	Epsilon-COP subunit of the coatomer	Epsilon-COP subunit of the coatomer; regulates retrograde Golgi-to-ER protein traffic; stabilizes Cop1p, the alpha-COP and the coatomer complex; non-essential for cell growth; protein abundance increases in response to DNA replication stress
S000001339	YIL077C	RCI37	Respiratory Chain Interacting protein of ~37 kDa	214991	215953	-1	Protein that associates with the large mitoribosomal subunit	Protein that associates with the large mitoribosomal subunit; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)
S000001340	YIL078W	THS1	THreonyl tRNA Synthetase	212499	214703	1	Threonyl-tRNA synthetase involved in tRNA aminoacylation	Threonyl-tRNA synthetase involved in tRNA aminoacylation; mRNA binding protein that preferentially binds to the mRNA of RNAPI subunits and DNA binding proteins; binds to an anticodon stem loop-like structure in RPC10 and impacts its translation; cytoplasmic protein; human homolog TARS can complement a yeast null mutant
S000001341	YIL079C	AIR1	Arginine methyltransferase-Interacting RING finger protein	210923	212005	-1	Zinc knuckle protein	Zinc knuckle protein; involved in nuclear RNA processing and degradation as a component of the TRAMP complex; stimulates the poly(A) polymerase activity of Pap2p in vitro; AIR1 has a paralog, AIR2, that arose from the whole genome duplication; although Air1p and Air2p are homologous TRAMP subunits, they have nonredundant roles in regulation of substrate specificity of the exosome
S000001345	YIL083C	CAB2	Coenzyme A Biosynthesis	203556	204653	-1	Phosphopantothenoylcysteine synthetase (PPCS)	Phosphopantothenoylcysteine synthetase (PPCS); catalyzes the second step of coenzyme A biosynthesis from pantothenate; subunit of the CoA-Synthesizing Protein Complex (CoA-SPC) that contains: Cab2p, Cab3p, Cab4p, Cab5p, Sis2p and Vhs3p subunits; null mutant lethality is complemented by human homolog PPCS and by E. coli coaBC, a bifunctional enzyme with PPCS activity
S000001346	YIL084C	SDS3	Suppressor of Defective Silencing	202276	203259	-1	Component of the Rpd3L histone deacetylase complex	Component of the Rpd3L histone deacetylase complex; required for its structural integrity and catalytic activity, involved in transcriptional silencing and required for sporulation; relocalizes to the cytosol in response to hypoxia; cells defective in SDS3 display pleiotropic phenotypes
S000001347	YIL085C	KTR7	Kre Two Related	200490	202043	-1	Putative mannosyltransferase involved in protein glycosylation	Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR7 has a paralog, KTR5, that arose from the whole genome duplication
S000001348	YIL086C	""	""	200153	200461	-1	Putative protein of unknown function	Putative protein of unknown function; conserved across S. cerevisiae strains
S000001349	YIL087C	AIM19	Altered Inheritance rate of Mitochondria	199646	200119	-1	Protein of unknown function	Protein of unknown function; mitochondrial protein that physically interacts with Tim23p; null mutant displays reduced respiratory growth
S000001350	YIL088C	AVT7	Amino acid Vacuolar Transport	197931	199403	-1	Vacuolar amino acid transporter	Vacuolar amino acid transporter; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
S000001351	YIL089W	""	""	195599	196216	1	Protein of unknown function found in the ER and vacuole lumen	Protein of unknown function found in the ER and vacuole lumen; overexpression of YIL089W affects endocytic protein trafficking
S000001352	YIL090W	ICE2	Inheritance of Cortical ER	193595	195070	1	Integral ER membrane protein with type-III transmembrane domains	Integral ER membrane protein with type-III transmembrane domains; promotes ER membrane expansion by inhibiting the Nem1-Spo7 phosphatase complex, resulting in reduced dephosphorylation and activation of Pah1p; maintains homeostasis during ER stress; required for post-translational SRP-independent protein translocation into the ER; necessary for efficient targeting of the Trm1p to the inner nuclear membrane; mutations cause defects in cortical ER morphology; member of the SERINC superfamily
S000001354	YIL092W	""	""	189066	190967	1	Putative protein of unknown function	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus
S000001355	YIL093C	RSM25	Ribosomal Small subunit of Mitochondria	187990	188784	-1	Mitochondrial ribosomal protein of the small subunit	Mitochondrial ribosomal protein of the small subunit
S000001356	YIL094C	LYS12	LYSine requiring	186517	187632	-1	Homo-isocitrate dehydrogenase	Homo-isocitrate dehydrogenase; an NAD-linked mitochondrial enzyme required for the fourth step in the biosynthesis of lysine, in which homo-isocitrate is oxidatively decarboxylated to alpha-ketoadipate
S000001357	YIL095W	PRK1	p53 Regulatory Kinase	183937	186369	1	Ser/Thr protein kinase	Ser/Thr protein kinase; regulates the organization and function of the actin cytoskeleton; inhibits clathrin-mediated endocytosis; phosphorylates the Pan1p and Sla1p subunits of the Pan1p-Sla1p-End3p complex, resulting in inhibition of complex formation; inhibits Scd5p through phosphorylation; phosphorylates Pan1p-interacting proteins, Ent1/2 and Yap1801/2; negatively regulated through autophosphorylation; functional overlap with ARK1
S000001358	YIL096C	BMT5	Base Methyltransferase of Twenty five S rRNA	182117	183127	-1	Methyltransferase required for m3U2634 methylation of the 25S rRNA	Methyltransferase required for m3U2634 methylation of the 25S rRNA; S-adenosylmethionine-dependent; associates with precursors of the 60S ribosomal subunit; predicted to be involved in ribosome biogenesis
S000001359	YIL097W	FYV10	Function required for Yeast Viability	180427	181977	1	Subunit of GID complex	Subunit of GID complex; involved in proteasome-dependent catabolite inactivation of gluconeogenic enzymes FBPase, PEPCK, and c-MDH; forms dimer with Rmd5p that is then recruited to GID Complex by Gid8p; contains a degenerate RING finger motif needed for GID complex ubiquitin ligase activity in vivo, as well as CTLH and CRA domains; plays role in anti-apoptosis; required for survival upon exposure to K1 killer toxin
S000001360	YIL098C	FMC1	Formation of Mitochondrial Complexes	179772	180239	-1	Mitochondrial matrix protein	Mitochondrial matrix protein; required for assembly or stability at high temperature of the F1 sector of mitochondrial F1F0 ATP synthase; null mutant temperature sensitive growth on glycerol is suppressed by multicopy expression of Odc1p
S000001361	YIL099W	SGA1	Sporulation-specific GlycoAmylase	178004	179653	1	Intracellular sporulation-specific glucoamylase	Intracellular sporulation-specific glucoamylase; involved in glycogen degradation; induced during starvation of a/a diploids late in sporulation, but dispensable for sporulation
S000001362	YIL100W	""	""	177373	177726	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the dubious ORF YIL100C-A
S000001363	YIL101C	XBP1	XhoI site-Binding Protein	175307	177250	-1	Transcriptional repressor	Transcriptional repressor; binds promoter sequences of cyclin genes, CYS3, and SMF2; not expressed during log phase of growth, but induced by stress or starvation during mitosis, and late in meiosis; represses 15% of all yeast genes as cells transition to quiescence; important for maintaining G1 arrest and for longevity of quiescent cells; member of Swi4p/Mbp1p family; phosphorylated by Cdc28p; relative distribution to nucleus increases upon DNA replication stress
S000001364	YIL102C	""	""	174582	174887	-1	Putative protein of unknown function	Putative protein of unknown function
S000001365	YIL103W	DPH1	DiPHthamide biosynthesis	171751	173028	1	Protein required for synthesis of diphthamide	Protein required for synthesis of diphthamide; required along with Dph2p, Kti11p, Jjj3p, and Dph5p; diphthamide is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); forms a complex with Dph2p that catalyzes the first step of diphthamide biosynthesis
S000001366	YIL104C	SHQ1	Small nucleolar RNAs of the box H/ACA family Quantitative accumulation	169982	171505	-1	Chaperone protein	Chaperone protein; required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing; functions as an RNA mimic; forms a complex with Naf1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; homology with known Hsp90p cochaperones; relocalizes to the cytosol in response to hypoxia
S000001367	YIL105C	SLM1	Synthetic Lethal with Mss4	167581	169641	-1	Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p	Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; TORC2 complex substrate and effector; protein abundance increases in response to DNA replication stress; SLM1 has a paralog, SLM2, that arose from the whole genome duplication
S000001369	YIL107C	PFK26	6-PhosphoFructo-2-Kinase	163278	165761	-1	6-phosphofructo-2-kinase	6-phosphofructo-2-kinase; inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate; has negligible fructose-2,6-bisphosphatase activity; transcriptional regulation involves protein kinase A
S000001370	YIL108W	""	""	160887	162977	1	Putative metalloendopeptidase	Putative metalloendopeptidase; forms cytoplasmic foci upon DNA replication stress
S000001372	YIL110W	HPM1	Histidine Protein Methyltransferase	156045	157178	1	AdoMet-dependent methyltransferase	AdoMet-dependent methyltransferase; involved in a novel 3-methylhistidine modification of multiple proteins, including ribosomal protein Rpl3p; seven beta-strand MTase family member; null mutant exhibits a weak vacuolar protein sorting defect and caspofungin resistance
S000001373	YIL111W	COX5B	Cytochrome c OXidase	155222	155765	1	Subunit Vb of cytochrome c oxidase	Subunit Vb of cytochrome c oxidase; cytochrome c oxidase is the terminal member of the mitochondrial inner membrane electron transport chain; Cox5Bp is predominantly expressed during anaerobic growth while its isoform Va (Cox5Ap) is expressed during aerobic growth; COX5B has a paralog, COX5A, that arose from the whole genome duplication
S000001374	YIL112W	HOS4	Hda One Similar	151595	154846	1	Subunit of the Set3 complex	Subunit of the Set3 complex; complex is a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; potential Cdc28p substrate
S000001375	YIL113W	SDP1	Stress-inducible Dual specificity Phosphatase	150559	151188	1	Stress-inducible dual-specificity MAP kinase phosphatase	Stress-inducible dual-specificity MAP kinase phosphatase; negatively regulates Slt2p MAP kinase by direct dephosphorylation, diffuse localization under normal conditions shifts to punctate localization after heat shock; SDP1 has a paralog, MSG5, that arose from the whole genome duplication
S000001376	YIL114C	POR2	PORin	149143	149988	-1	Putative mitochondrial porin (voltage-dependent anion channel)	Putative mitochondrial porin (voltage-dependent anion channel); not required for mitochondrial membrane permeability or mitochondrial osmotic stability; POR2 has a paralog, POR1, that arose from the whole genome duplication
S000001377	YIL115C	NUP159	NUclear Pore	144327	148709	-1	FG-nucleoporin component of central core of the nuclear pore complex	FG-nucleoporin component of central core of the nuclear pore complex; also part of the nuclear pore complex (NPC) cytoplasmic filaments; contributes directly to nucleocytoplasmic transport; regulates ADP release from the ATP-dependent RNA helicase Dbp5p; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup116p)
S000001378	YIL116W	HIS5	HIStidine requiring	142928	144085	1	Histidinol-phosphate aminotransferase	Histidinol-phosphate aminotransferase; catalyzes the seventh step in histidine biosynthesis; responsive to general control of amino acid biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts
S000001379	YIL117C	PRM5	Pheromone-Regulated Membrane protein	140613	141569	-1	Pheromone-regulated protein, predicted to have 1 transmembrane segment	Pheromone-regulated protein, predicted to have 1 transmembrane segment; induced during cell integrity signaling; PRM5 has a paralog, YNL058C, that arose from the whole genome duplication
S000001380	YIL118W	RHO3	Ras HOmolog	139752	140447	1	Non-essential small GTPase of the Rho/Rac family of Ras-like proteins	Non-essential small GTPase of the Rho/Rac family of Ras-like proteins; involved in the establishment of cell polarity; GTPase activity positively regulated by the GTPase activating protein (GAP) Rgd1p
S000001381	YIL119C	RPI1	Ras-cAMP Pathway Inhibitor	136654	137877	-1	Transcription factor, allelic differences between S288C and Sigma1278b	Transcription factor, allelic differences between S288C and Sigma1278b; mediates fermentation stress tolerance by modulating cell wall integrity; overexpression suppresses heat shock sensitivity of wild-type RAS2 overexpression and also suppresses cell lysis defect of mpk1 mutation; allele from S288c can confer fMAPK pathway independent transcription of FLO11; S288C and Sigma1278b alleles differ in number of tandem repeats within ORF
S000001382	YIL120W	QDR1	QuiniDine Resistance	134417	136108	1	Multidrug transporter of the major facilitator superfamily	Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; involved in spore wall assembly; sequence similarity to DTR1 and QDR3, and the triple mutant dtr1 qdr1 qdr3 exhibits reduced dityrosine fluorescence relative to the single mutants; required for resistance to quinidine, ketoconazole, fluconazole, and barban; QDR1 has a paralog, AQR1, that arose from the whole genome duplication
S000001383	YIL121W	QDR2	QuiniDine Resistance	132244	133872	1	Plasma membrane transporter of the major facilitator superfamily	Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; exports copper; has broad substrate specificity and can transport many mono- and divalent cations; transports a variety of drugs and is required for resistance to quinidine, barban, cisplatin, and bleomycin; contributes to potassium homeostasis; expression is regulated by copper
S000001384	YIL122W	POG1	Promoter Of Growth	130610	131665	1	DNA-binding transcriptional activator	DNA-binding transcriptional activator; involved in cell cycle regulation; overexpression promotes recovery from pheromone induced arrest via CLN1/2 transcription, induction of of IME1 during sporulation, and suppression of stress sensitivity resulting from mutation of the E3 ubiquitin ligase Rsp5p; binds upstream of BAR1 and cell cycle-related genes; phosphorylated form may be ubiquitinated by Dma2p; potential Cdc28p substrate; regulated by Swi4/6 cell-cycle box binding factor (SBF)
S000001385	YIL123W	SIM1	Start Independent of Mitosis	128151	129581	1	Protein of the SUN family (Sim1p, Uth1p, Nca3p, Sun4p)	Protein of the SUN family (Sim1p, Uth1p, Nca3p, Sun4p); may participate in DNA replication; promoter contains SCB regulation box at -300 bp indicating that expression may be cell cycle-regulated; SIM1 has a paralog, SUN4, that arose from the whole genome duplication
S000001386	YIL124W	AYR1	1-AcyldihYdroxyacetone-phosphate Reductase	126204	127097	1	Bifunctional triacylglycerol lipase and 1-acyl DHAP reductase	Bifunctional triacylglycerol lipase and 1-acyl DHAP reductase; NADPH-dependent 1-acyl dihydroxyacetone phosphate reductase involved in phosphatidic acid biosynthesis; lipid droplet triacylglycerol lipase involved in mobilization of non-polar lipids; found in lipid particles, endoplasmic reticulum and mitochondrial outer membrane; forms NADPH-regulated channel in mitochondrial outer membrane; required for spore germination; role in cell wall biosynthesis; capable of metabolizing steroid hormones
S000001387	YIL125W	KGD1	alpha-KetoGlutarate Dehydrogenase	122689	125733	1	Subunit of the mitochondrial alpha-ketoglutarate dehydrogenase complex	Subunit of the mitochondrial alpha-ketoglutarate dehydrogenase complex; catalyzes a key step in the tricarboxylic acid (TCA) cycle, the oxidative decarboxylation of alpha-ketoglutarate to form succinyl-CoA
S000001388	YIL126W	STH1	SNF Two Homolog	117992	122071	1	ATPase component of the RSC chromatin remodeling complex	ATPase component of the RSC chromatin remodeling complex; required for expression of early meiotic genes; promotes base excision repair in chromatin; essential helicase-related protein homologous to Snf2p
S000001389	YIL127C	RRT14	Regulator of rDNA Transcription	117024	117644	-1	Putative protein of unknown function	Putative protein of unknown function; identified in a screen for mutants with decreased levels of rDNA transcription; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; predicted to be involved in ribosome biogenesis
S000001390	YIL128W	MET18	METhionine requiring	113806	116904	1	Component of cytosolic iron-sulfur protein assembly (CIA) machinery	Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at a late step of Fe-S cluster assembly; forms the CIA targeting complex with Cia1p and Cia2p that directs Fe-S cluster incorporation and maturation of a subset of cytosolic and nuclear proteins involved in methionine biosynthesis, DNA replication and repair, transcription, and telomere maintenance; ortholog of human MMS19
S000001391	YIL129C	TAO3	Transcriptional Activator of OCH1	106107	113237	-1	Component of the RAM signaling network	Component of the RAM signaling network; is involved in regulation of Ace2p activity and cellular morphogenesis, interacts with protein kinase Cbk1p and also with Kic1p
S000001392	YIL130W	ASG1	Activator of Stress Genes	102782	105676	1	Zinc cluster protein proposed to be a transcriptional regulator	Zinc cluster protein proposed to be a transcriptional regulator; regulator involved in the stress response; regulates utilization of fatty acids and accumulation of lipids
S000001393	YIL131C	FKH1	ForK head Homolog	100781	102235	-1	Forkhead family transcription factor	Forkhead family transcription factor; rate-limiting replication origin activator; evolutionarily conserved lifespan regulator; binds multiple chromosomal elements with distinct specificities, cell cycle dynamics; regulates transcription elongation, chromatin silencing at mating loci, expression of G2/M phase genes; facilitates clustering, activation of early-firing replication origins; binds HML recombination enhancer, regulates donor preference during mating-type switching
S000001394	YIL132C	CSM2	Chromosome Segregation in Meiosis	99860	100501	-1	Subunit of the Shu complex (aka PCSS complex)	Subunit of the Shu complex (aka PCSS complex); Shu complex also includes Psy3, Shu1, Shu2, and promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p anti-recombinase function; promotes formation of Rad51p filaments; Psy3p and Csm2p contain similar DNA-binding regions which work together to form a single DNA binding site; required for accurate chromosome segregation during meiosis
S000001395	YIL133C	RPL16A	Ribosomal Protein of the Large subunit	98527	99416	-1	Ribosomal 60S subunit protein L16A	Ribosomal 60S subunit protein L16A; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16A has a paralog, RPL16B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
S000001396	YIL134W	FLX1	FLavin eXchange	97395	98330	1	Mitochondrial flavin adenine dinucleotide transporter	Mitochondrial flavin adenine dinucleotide transporter; FAD is a synthesis product of riboflavin; human homolog SLC25A32 is implicated in multiple acyl-CoA dehydrogenase deficiency (MADD) or glutaric aciduria type II (GAII), and can complement yeast null mutant
S000001397	YIL135C	VHS2	Viable in a Hal3 Sit4 background	95065	96375	-1	Regulator of septin dynamics	Regulator of septin dynamics; involved in the regulation of septin dynamics at bud neck after mitotic entry, likely by stabilizing septin structure; regulated at post-translational level by cell cycle dependent phosphorylation; likely phosphorylated by Cdc28p and dephosphorylated by Cdc14p before cytokinesis; high-copy suppressor of synthetic lethality of sis2 sit4 double mutant; VHS2 has a paralog, MLF3, that arose from the whole genome duplication
S000001398	YIL136W	OM45	Outer Membrane	93619	94800	1	Mitochondrial outer membrane protein of unknown function	Mitochondrial outer membrane protein of unknown function; major constituent of the outer membrane, extending into the intermembrane space; interacts with porin (Por1p) and with Om14p; imported via the presequence pathway involving the TOM and TIM23 complexes, then assembled in the outer membrane by Mim1p; protein abundance increases in response to DNA replication stress
S000001399	YIL137C	TMA108	Translation Machinery Associated	89948	92788	-1	Ribosome-associated, nascent chain binding factor	Ribosome-associated, nascent chain binding factor; binds N-terminal region of nascent peptides during translation; recognizes target proteins via its putative metallopeptidase peptide-binding pocket
S000001400	YIL138C	TPM2	TroPoMyosin	89230	89715	-1	Minor isoform of tropomyosin	Minor isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; appears to have distinct and also overlapping functions with Tpm1p; TPM2 has a paralog, TPM1, that arose from the whole genome duplication
S000001401	YIL139C	REV7	REVersionless	87979	88716	-1	Accessory subunit of DNA polymerase zeta (pol zeta)	Accessory subunit of DNA polymerase zeta (pol zeta); involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair; may be involved in meiosis; forms a complex with Rev3p, Pol31p and Pol32p
S000001402	YIL140W	AXL2	AXiaL 2 bud site selection	85366	87837	1	Integral plasma membrane protein	Integral plasma membrane protein; required for axial budding in haploid cells; localizes to the incipient bud site and bud neck; glycosylated by Pmt4p; potential Cdc28p substrate
S000001403	YIL141W	""	""	85053	85442	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000001404	YIL142W	CCT2	Chaperonin Containing TCP-1	83302	84885	1	Subunit beta of the cytosolic chaperonin Cct ring complex	Subunit beta of the cytosolic chaperonin Cct ring complex; related to Tcp1p, required for the assembly of actin and tubulins in vivo
S000001406	YIL144W	NDC80	Nuclear Division Cycle	78074	80149	1	Component of the kinetochore-associated Ndc80 complex	Component of the kinetochore-associated Ndc80 complex; conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, and kinetochore assembly and clustering; evolutionarily conserved; complex members include Ndc80p, Nuf2p, Scp24p, and Spc25p; modified by sumoylation
S000001407	YIL145C	PAN6	PANtothenate biosynthesis	76354	77283	-1	Pantothenate synthase	Pantothenate synthase; also known as pantoate-beta-alanine ligase, required for pantothenic acid biosynthesis, deletion causes pantothenic acid auxotrophy, homologous to E. coli panC
S000001408	YIL146C	ATG32	AuTophaGy related	74184	75773	-1	Mitochondrial outer membrane protein required to initiate mitophagy	Mitochondrial outer membrane protein required to initiate mitophagy; recruits the autophagy adaptor protein Atg11p and the ubiquitin-like protein Atg8p to the mitochondrial surface to initiate mitophagy, the selective vacuolar degradation of mitochondria in response to starvation; can promote pexophagy when placed ectopically in the peroxisomal membrane; regulates mitophagy and ethanol production during alcoholic fermentation
S000001409	YIL147C	SLN1	Synthetic Lethal of N-end rule	69791	73453	-1	Transmembrane histidine phosphotransfer kinase and osmosensor	Transmembrane histidine phosphotransfer kinase and osmosensor; regulates MAP kinase cascade; transmembrane protein with an intracellular kinase domain that signals to Ypd1p and Ssk1p, thereby forming a phosphorelay system similar to bacterial two-component regulators
S000001410	YIL148W	RPL40A	Ribosomal Protein of the Large subunit	68708	69528	1	Ubiquitin-ribosomal 60S subunit protein L40A fusion protein	Ubiquitin-ribosomal 60S subunit protein L40A fusion protein; cleaved to yield ubiquitin and ribosomal protein L40A; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40A has a paralog, RPL40B, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
S000001411	YIL149C	MLP2	Myosin-Like Protein	63028	68067	-1	Myosin-like protein associated with the nuclear envelope	Myosin-like protein associated with the nuclear envelope; nuclear basket protein that connects the nuclear pore complex with the nuclear interior; involved in the Tel1p pathway that controls telomere length; MLP2 has a paralog, MLP1, that arose from the whole genome duplication
S000001412	YIL150C	MCM10	MiniChromosome Maintenance	61013	62728	-1	Essential chromatin-associated protein	Essential chromatin-associated protein; involved in initiation of DNA replication; required for association of MCM2-7 complex with replication origins; required to stabilize catalytic subunit of DNA polymerase-alpha; coordinates function of replication fork helicase; self-associates through its N-terminal domain
S000001414	YIL152W	VPR1	Viable Products in spp1∆ meiosis Reduced	56545	57252	1	Putative protein of unknown function	Putative protein of unknown function
S000001415	YIL153W	RRD1	Resistant to Rapamycin Deletion	55198	56379	1	Peptidyl-prolyl cis/trans-isomerase	Peptidyl-prolyl cis/trans-isomerase; activator of the phosphotyrosyl phosphatase activity of PP2A; involved in G1 phase progression, microtubule dynamics, bud morphogenesis and DNA repair; required for rapid reduction of Sgs1p levels in response to rapamycin; subunit of the Tap42p-Sit4p-Rrd1p complex; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress
S000001416	YIL154C	IMP21	Independent of Mitochondrial Particle	53981	55021	-1	Transcriptional activator involved in maintenance of ion homeostasis	Transcriptional activator involved in maintenance of ion homeostasis; also involved in protection against DNA damage caused by bleomycin and other oxidants; contains a C-terminal leucine-rich repeat
S000001417	YIL155C	GUT2	Glycerol UTilization	51759	53708	-1	Mitochondrial glycerol-3-phosphate dehydrogenase	Mitochondrial glycerol-3-phosphate dehydrogenase; expression is repressed by both glucose and cAMP and derepressed by non-fermentable carbon sources in a Snf1p, Rsf1p, Hap2/3/4/5 complex dependent manner
S000001418	YIL156W	UBP7	UBiquitin-specific Protease	48091	51306	1	Ubiquitin-specific protease that cleaves ubiquitin-protein fusions	Ubiquitin-specific protease that cleaves ubiquitin-protein fusions; involved in cell cycle progression through S phase; UBP7 has a paralog, UBP11, that arose from the whole genome duplication
S000001419	YIL157C	COA1	Cytochrome Oxidase Assembly	46949	47542	-1	Mitochondrial inner membrane protein	Mitochondrial inner membrane protein; required for assembly of the cytochrome c oxidase complex (complex IV); interacts with complex IV assembly factor Shy1p during the early stages of assembly
S000001420	YIL158W	AIM20	Altered Inheritance rate of Mitochondria	46201	46815	1	Protein of unknown function	Protein of unknown function; overexpression causes cell cycle delay or arrest; green fluorescent protein (GFP)-fusion protein localizes to vacuole; null mutant displays elevated frequency of mitochondrial genome loss; relocalizes from nucleus to cytoplasm upon DNA replication stress; AIM20 has a paralog, SKG1, that arose from the whole genome duplication
S000001421	YIL159W	BNR1	BNi1 Related	41825	45952	1	Formin	Formin; nucleates the formation of linear actin filaments; involved in processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables; activity is regulated by Hof1p and by the Bud14p-Kel1p-Kel2p complex; dephosphorylated and delocalized from the division site in a Glc7p/Ref2p-dependent manner; functionally redundant with BNI1
S000001422	YIL160C	POT1	Peroxisomal Oxoacyl Thiolase	40191	41444	-1	3-ketoacyl-CoA thiolase with broad chain length specificity	3-ketoacyl-CoA thiolase with broad chain length specificity; cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids
S000001423	YIL161W	SMU2	Stalled Monosome Ubiquitination	39433	40140	1	Protein involved in removal of stalled ribosomes	Protein involved in removal of stalled ribosomes; acts as cofactor for Fap1p and Fpr1p in ubiquitination of stalled monosomes and subsequent degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; mRNA is enriched in Scp160p-associated mRNPs; YIL161W is a non-essential gene
S000001424	YIL162W	SUC2	SUCrose	37385	38983	1	Invertase	Invertase; sucrose hydrolyzing enzyme; a secreted, glycosylated form is regulated by glucose repression, and an intracellular, nonglycosylated enzyme is produced constitutively
S000001425	YIL163C	""	""	36899	37252	-1	Protein of unknown function	Protein of unknown function; mRNA identified as translated by ribosome profiling data
S000001426	YIL164C	NIT1	NITrilase superfamily	34087	34686	-1	Nitrilase	Nitrilase; member of the nitrilase branch of the nitrilase superfamily; in closely related species and other S. cerevisiae strain backgrounds YIL164C and adjacent ORF, YIL165C, likely constitute a single ORF encoding a nitrilase gene
S000001427	YIL165C	""	""	33718	34077	-1	Putative protein of unknown function	Putative protein of unknown function; mutant exhibits mitophagy defects; in closely related species and other S. cerevisiae strain backgrounds YIL165C and adjacent ORF, YIL164C, likely constitute a single ORF encoding a nitrilase gene
S000001428	YIL166C	SOA1	SulfOnAte transport	30938	32566	-1	Sulfonate and inorganic sulfur transporter	Sulfonate and inorganic sulfur transporter; low affinity sulfate, high affinity sulfite, thiosulfite and major sulfonate transporter; functions as a an H+ symporter; similar to the allantoate permease (Dal5p) subfamily of the major facilitator superfamily; mRNA expression is elevated by sulfur limitation; non-essential gene
S000001429	YIL167W	SDL1	""	29032	30048	1	Blocked reading frame otherwise encoding L-serine dehydratase	Blocked reading frame otherwise encoding L-serine dehydratase
S000001430	YIL168W	""	""	29032	29415	1	Open reading frame unlikely to produce a functional protein in S288C	Open reading frame unlikely to produce a functional protein in S288C; in closely related species and other S. cerevisiae strain backgrounds YIL168W and adjacent ORF, YIL167W, constitute a single ORF encoding L-serine dehydratase
S000001432	YIL170W	HXT12	HeXose Transporter	19847	21220	1	Possible pseudogene in strain S288C	Possible pseudogene in strain S288C; YIL170W/HXT12 and the adjacent ORF, YIL171W, together encode a non-functional member of the hexose transporter family
S000001433	YIL171W	""	""	19515	19844	1	Possible pseudogene in strain S288C	Possible pseudogene in strain S288C; YIL171W and the adjacent ORF, YIL170W/HXT12, together encode a non-functional member of the hexose transporter family
S000001435	YIL173W	VTH1	Vps Ten Homolog	11492	16141	1	Putative membrane glycoprotein	Putative membrane glycoprotein; has strong similarity to Vth2p and Pep1p/Vps10p; may be involved in vacuolar protein sorting
S000001440	YIR001C	SGN1	Slower Growth on Non-fermentable carbon sources	356143	356895	-1	Cytoplasmic RNA-binding protein	Cytoplasmic RNA-binding protein; contains an RNA recognition motif (RRM); may have a role in mRNA translation, as suggested by genetic interactions with genes encoding proteins involved in translational initiation
S000001441	YIR002C	MPH1	Mutator PHenotype	357415	360396	-1	3'-5' DNA helicase involved in error-free bypass of DNA lesions	3'-5' DNA helicase involved in error-free bypass of DNA lesions; binds flap DNA, stimulates activity of Rad27p and Dna2p; prevents crossovers between ectopic sequences by removing substrates for Mus81-Mms4 or Rad1-Rad10 cleavage; homolog of human FANCM Fanconi anemia protein that is involved in stabilizing and remodeling blocked replication forks; member of SF2 DExD/H superfamily of helicases; nonsense or missense mutations in FANCM can make people more likely to get cancer
S000001442	YIR003W	AIM21	Altered Inheritance rate of Mitochondria	360885	362924	1	Subunit of a complex that associates with actin filaments	Subunit of a complex that associates with actin filaments; forms a complex with Tda2p that inhibits barbed end F-actin assembly; elevates actin monomer pools to increase endocytotic efficiency and to regulate the distribution of actin between cables and patches; Aim21p/Tda2p forms a larger complex with actin capping proteins Cap1p and Cap2p; involved in mitochondrial migration along actin filaments; recruited to cortical actin patches by SH3 domain-containing proteins Bbc1p and Abp1p
S000001443	YIR004W	DJP1	DnaJ Protein	363221	364519	1	ER-associated chaperone involved in protein targeting	ER-associated chaperone involved in protein targeting; redirects mitochondrial membrane protein precursors to mitochondrial translocation system; required for peroxisomal protein import and involved in peroxisome assembly; facilitates import of Mim1p and Mim2p into the mitochondrial outer membrane; homologous to E. coli DnaJ
S000001444	YIR005W	IST3	Increased Sodium Tolerance	364889	365335	1	Component of the U2 snRNP	Component of the U2 snRNP; required for the first catalytic step of splicing and for spliceosomal assembly; interacts with Rds3p and is required for Mer1p-activated splicing; diploid mutants have a specific defect in MATa1 pre-mRNA splicing which leads to haploid gene expression in diploids
S000001445	YIR006C	PAN1	Poly(A)-binding protein-dependent poly(A) riboNuclease	365466	369908	-1	Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p	Part of actin cytoskeleton-regulatory complex Pan1p-Sla1p-End3p; associates with actin patches on cell cortex; promotes protein-protein interactions essential for endocytosis; regulates late stages of endocytosis; binds to and activates Arp2/3 complex in vitro; phosphorylation of Thr-1225 is regulated by MAPK Hog1p in response to osmotic stress
S000001446	YIR007W	EGH1	Cryptococcus neoformans EGCrP2 Homolog	370704	372998	1	Steryl-beta-glucosidase with broad specificity for aglycones	Steryl-beta-glucosidase with broad specificity for aglycones; has a role in ergosteryl-beta-glucoside catabolism; required for normal vacuolar morphology; has similarity to the C. neoformans ergosteryl-beta-glucosidase EGCrP2; localizes to the cytosol
S000001447	YIR008C	PRI1	DNA PRImase	373077	374306	-1	Subunit of DNA primase	Subunit of DNA primase; DNA primase is required for DNA synthesis and double-strand break repair
S000001448	YIR009W	MSL1	MUD Synthetic Lethal	374525	374860	1	U2B component of U2 snRNP	U2B component of U2 snRNP; involved in splicing, binds the U2 snRNA stem-loop IV in vitro but requires association of Lea1p for in vivo binding; does not contain the conserved C-terminal RNA binding domain found in other family members
S000001449	YIR010W	DSN1	Dosage Suppressor of NNF1	375431	377161	1	Essential component of the outer kinetochore MIND complex	Essential component of the outer kinetochore MIND complex; joins kinetochore subunits contacting DNA to those contacting microtubules; phosphorylation promotes interaction between outer and inner kinetochore proteins; kinetochore receptor for monopolin, via interaction with Csm1p; essential for both meiotic and mitotic chromosome segregation; MIND complex consists of Mtw1p, Nnf1p, Nsl1p and Dsn1p; phosphorylated by monopolin subunit, Hrr25p and Aurora kinase, Ipl1p; modified by sumoylation
S000001451	YIR012W	SQT1	Suppressor of QSR1 Truncations	378486	379781	1	Specific assembly chaperone for ribosomal protein Rpl10p	Specific assembly chaperone for ribosomal protein Rpl10p; co-translationally associates with nascent Rpl10p, preventing aggregation; involved in biogenesis of the ribosomal large subunit; contains multiple WD repeats; interacts genetically and physically with Qsr1p; protein abundance increases in response to DNA replication stress
S000001452	YIR013C	GAT4	""	380019	380384	-1	Protein containing GATA family zinc finger motifs	Protein containing GATA family zinc finger motifs; involved in spore wall assembly; sequence similarity to GAT3, and the double mutant gat3 gat4 exhibits reduced dityrosine fluorescence relative to the single mutants
S000001453	YIR014W	VLD1	Vacuole Localized Dsc protein	381086	381814	1	Component of Dsc E3 ligase complex in vacuolar membranes	Component of Dsc E3 ligase complex in vacuolar membranes; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p; YIR014W is a non-essential gene
S000001454	YIR015W	RPR2	RNase P Ribonucleoprotein	381948	382382	1	Subunit of nuclear RNase P	Subunit of nuclear RNase P; nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; not shared between RNase MRP and RNase P, in contrast to all other RNase P protein subunits; increases the activity and thermal stability of the complex, along with Pop2p; protein abundance increases in response to DNA replication stress
S000001455	YIR016W	""	""	382628	383425	1	Putative protein of unknown function	Putative protein of unknown function; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p; overexpression causes a cell cycle delay or arrest; non-essential gene; YIR016W has a paralog, YOL036W, that arose from the whole genome duplication
S000001456	YIR017C	MET28	METhionine	383556	384119	-1	bZIP transcriptional activator in the Cbf1p-Met4p-Met28p complex	bZIP transcriptional activator in the Cbf1p-Met4p-Met28p complex; participates in the regulation of sulfur metabolism
S000001457	YIR018W	YAP5	Yeast AP-1	384609	385346	1	Basic leucine zipper (bZIP) iron-sensing transcription factor	Basic leucine zipper (bZIP) iron-sensing transcription factor; senses high-iron conditions via two Fe/S clusters bound to its activator domain; involved in diauxic shift; YAP5 has a paralog, YAP7, that arose from the whole genome duplication
S000001458	YIR019C	FLO11	FLOcculation	389572	393675	-1	GPI-anchored cell surface glycoprotein (flocculin)	GPI-anchored cell surface glycoprotein (flocculin); required for pseudohyphal and invasive growth, flocculation, and biofilm formation; major determinant of colony morphology; QTL that controls chronological life span; carries intragenic tandem repeats that are expanded in different strains; required for formation of fibrous interconnections between cells; role in co-flocculation with other yeast species; cleaved and shed from cells, contributing to their surface properties
S000001459	YIR020C	""	""	394255	394557	-1	Protein of unknown function	Protein of unknown function; mRNA identified as translated by ribosome profiling data; SWAT-GFP fusion protein localizes to the endoplasmic reticulum
S000001460	YIR021W	MRS1	Mitochondrial RNA Splicing	397294	398385	1	Splicing protein	Splicing protein; required for splicing of two mitochondrial group I introns (BI3 in COB and AI5beta in COX1); forms a splicing complex, containing four subunits of Mrs1p and two subunits of the BI3-encoded maturase, that binds to the BI3 RNA; MRS1 has a paralog, CCE1, that arose from the whole genome duplication
S000001461	YIR022W	SEC11	SECretory	398733	399236	1	18kDa catalytic subunit of the Signal Peptidase Complex (SPC)	18kDa catalytic subunit of the Signal Peptidase Complex (SPC); the Signal Peptidase Complex cleaves the signal sequence of proteins targeted to the endoplasmic reticulum; other members are Spc1p, Spc2p, Spc3p, and Sec11p
S000001462	YIR023W	DAL81	Degradation of Allantoin	399777	402689	1	Positive regulator of genes in multiple nitrogen degradation pathways	Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
S000001463	YIR024C	INA22	INner membrane Assembly 22 kDa	402841	403491	-1	F1F0 ATP synthase peripheral stalk assembly factor	F1F0 ATP synthase peripheral stalk assembly factor; subunit of the matrix-exposed inner mitochondrial membrane localized INA complex (Ina22p-Ina17p) involved in assembly of the F1F0 peripheral stalk; co-purifies with Aim43p, ATP synthase subunits, and cytochrome bc1 complex assembly factors; interacts with Arh1p, a mitochondrial oxidoreductase; deletion mutant has a respiratory growth defect
S000001464	YIR025W	MND2	Meiotic Nuclear Divisions	403659	404765	1	Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C)	Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); necessary for maintaining sister chromatid cohesion in prophase I of meiosis by inhibiting premature ubiquitination and subsequent degradation of substrates by the APC(Ama1) ubiquitin ligase
S000001465	YIR026C	YVH1	Yeast vaccinia virus VH1 Homolog	404873	405967	-1	Dual specificity protein phosphatase	Dual specificity protein phosphatase; regulates growth, sporulation, and glycogen accumulation in a cAMP-dependent protein kinase cascade dependent manner; mutants are defective in 60S ribosome assembly; positively regulates pre-autophagosomal structure (PAS) formation upon nitrogen starvation or rapamycin treatment
S000001466	YIR027C	DAL1	Degradation of Allantoin	406260	407642	-1	Allantoinase	Allantoinase; converts allantoin to allantoate in the first step of allantoin degradation; expression sensitive to nitrogen catabolite repression
S000001467	YIR028W	DAL4	Degradation of Allantoin	408468	410375	1	Allantoin permease	Allantoin permease; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation
S000001468	YIR029W	DAL2	Degradation of Allantoin	410807	411838	1	Allantoicase	Allantoicase; converts allantoate to urea and ureidoglycolate in the second step of allantoin degradation; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation
S000001469	YIR030C	DCG1	Dal80p-Controlled Gene	412036	412770	-1	Protein of unknown function	Protein of unknown function; expression is sensitive to nitrogen catabolite repression and regulated by Dal80p; contains transmembrane domain
S000001470	YIR031C	DAL7	Degradation of Allantoin	413015	414679	-1	Malate synthase	Malate synthase; can accept butyryl-CoA as acyl-CoA donor in addition to traditional substrate acetyl-CoA; recycles glyoxylate generated during allantoin degradation; SWAT-GFP and mCherry fusion proteins localize to the cytosol; expression sensitive to nitrogen catabolite repression and induced by allophanate, an intermediate in allantoin degradation
S000001471	YIR032C	DAL3	Degradation of Allantoin	415030	415617	-1	Ureidoglycolate lyase	Ureidoglycolate lyase; converts ureidoglycolate to glyoxylate and urea in the third step of allantoin degradation; expression is sensitive to nitrogen catabolite repression; this enzyme is sometimes referred to 'ureidoglycolate hydrolase' but should not be confused with the Arabidopsis thaliana ureidoglycolate hydrolase enzyme which converts ureidoglycolate to glyoxylate, ammonia and carbon dioxide
S000001472	YIR033W	MGA2	Multicopy suppressor of GAm1 (snf2)	416124	419465	1	ER membrane protein involved in regulation of OLE1 transcription	ER membrane protein involved in regulation of OLE1 transcription; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting; MGA2 has a paralog, SPT23, that arose from the whole genome duplication
S000001473	YIR034C	LYS1	LYSine requiring	419615	420736	-1	Saccharopine dehydrogenase (NAD+, L-lysine-forming)	Saccharopine dehydrogenase (NAD+, L-lysine-forming); catalyzes the conversion of saccharopine to L-lysine, which is the final step in the lysine biosynthesis pathway; contains mRNA binding activity; required for targeting of Pls1p to the peroxisomal matrix in the absence of lysine
S000001474	YIR035C	NRE1	Novel REductase	421026	421790	-1	Benzil oxidoreductase with preference for NADPH	Benzil oxidoreductase with preference for NADPH; localizes to the cytoplasm; sequence similarity with short-chain dehydrogenase/reductases; putative paralog of IRC24
S000001475	YIR036C	IRC24	Increased Recombination Centers	422074	422865	-1	Benzil oxidoreductase with preference for NADPH	Benzil oxidoreductase with preference for NADPH; localizes to the cytoplasm; induced by the DNA-damaging agent MMS; null mutant has elevated levels of spontaneous Rad52p foci; sequence similarity with short-chain dehydrogenase/reductases; putative paralog of IRC24
S000001476	YIR037W	HYR1	HYdroperoxide Resistance	423128	423619	1	Glutathione peroxidase	Glutathione peroxidase; functions as hydroperoxide receptor to sense intracellular hydroperoxide levels and transduce redox signal to Yap1p transcription factor; glutathione peroxidase activity is neuroprotective in models of Huntington's disease; HYR1 has a paralog, GPX1, that arose from the whole genome duplication
S000001477	YIR038C	GTT1	GlutaThione Transferase	423809	424513	-1	ER associated glutathione S-transferase	ER associated glutathione S-transferase; capable of homodimerization; glutathione transferase for Yvc1p vacuolar cation channel; expression induced during the diauxic shift and throughout stationary phase; functional overlap with Gtt2p, Grx1p, and Grx2p
S000001478	YIR039C	YPS6	YaPSin	430498	432111	-1	Putative GPI-anchored aspartic protease	Putative GPI-anchored aspartic protease; member of the yapsin family of proteases involved in cell wall growth and maintenance
S000001481	YIR042C	""	""	435273	435983	-1	Putative protein of unknown function	Putative protein of unknown function; YIR042C is a non-essential gene
S000001482	YIR043C	""	""	437043	438179	-1	Blocked reading frame	Blocked reading frame; may encode a non-functional member of the conserved, often subtelomerically-encoded Cos protein family
S000001483	YIR044C	""	""	437994	438179	-1	Possible pseudogene in strain S288C	Possible pseudogene in strain S288C; YIR044C and the adjacent ORF, YIR043C, together may encode a non-functional member of the conserved, often subtelomerically-encoded Cos protein family
S000001484	YKL001C	MET14	METhionine requiring	438777	439385	-1	Adenylylsulfate kinase	Adenylylsulfate kinase; required for sulfate assimilation and involved in methionine metabolism; human homolog PAPSS2 complements yeast null mutant
S000001485	YKL002W	DID4	Doa4-Independent Degradation	437778	438544	1	Class E Vps protein of the ESCRT-III complex	Class E Vps protein of the ESCRT-III complex; required for sorting of integral membrane proteins into lumenal vesicles of multivesicular bodies, and for delivery of newly synthesized vacuolar enzymes to the vacuole, involved in endocytosis
S000001486	YKL003C	MRP17	Mitochondrial Ribosomal Protein	437097	437492	-1	Mitochondrial ribosomal protein of the small subunit	Mitochondrial ribosomal protein of the small subunit; MRP17 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator
S000001488	YKL005C	BYE1	BYpass of ESS1	433093	434877	-1	Negative regulator of transcription elongation	Negative regulator of transcription elongation; contains a TFIIS-like domain that associates with chromatin and a PHD domain that interacts with H3K4me3; multicopy suppressor of temperature-sensitive ess1 mutations, binds RNA polymerase II large subunit
S000001489	YKL006W	RPL14A	Ribosomal Protein of the Large subunit	431906	432720	1	Ribosomal 60S subunit protein L14A	Ribosomal 60S subunit protein L14A; N-terminally acetylated; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14A has a paralog, RPL14B, that arose from the whole genome duplication
S000001490	YKL007W	CAP1	CAPping	429302	430108	1	Alpha subunit of the capping protein heterodimer (Cap1p and Cap2p)	Alpha subunit of the capping protein heterodimer (Cap1p and Cap2p); capping protein (CP) binds to the barbed ends of actin filaments preventing further polymerization; localized predominantly to cortical actin patches; protein increases in abundance and relocalizes from bud neck to plasma membrane upon DNA replication stress
S000001491	YKL008C	LAC1	Longevity-Assurance gene Cognate (LAG1 Cognate)	427295	428551	-1	Ceramide synthase component	Ceramide synthase component; involved in synthesis of ceramide from C26(acyl)-coenzyme A and dihydrosphingosine or phytosphingosine, functionally equivalent to Lag1p; LAC1 has a paralog, LAG1, that arose from the whole genome duplication
S000001492	YKL009W	MRT4	mRNA Turnover 4	426242	426952	1	Protein involved in mRNA turnover and ribosome assembly	Protein involved in mRNA turnover and ribosome assembly; required at post-transcriptional step for efficient retrotransposition; localizes to the nucleolus
S000001493	YKL010C	UFD4	Ubiquitin Fusion Degradation protein	421424	425875	-1	Ubiquitin-protein ligase (E3)	Ubiquitin-protein ligase (E3); interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome; involved in K29-linked ubiquitin-dependent protein catabolism, including ubiquitin fusion proteins; relative distribution to the nucleus increases upon DNA replication stress
S000001494	YKL011C	CCE1	Cruciform Cutting Endonuclease	420155	421216	-1	Mitochondrial cruciform cutting endonuclease	Mitochondrial cruciform cutting endonuclease; cleaves Holliday junctions formed during recombination of mitochondrial DNA; CCE1 has a paralog, MRS1, that arose from the whole genome duplication
S000001495	YKL012W	PRP40	Pre-mRNA Processing	418310	420061	1	U1 snRNP protein involved in splicing	U1 snRNP protein involved in splicing; interacts with the branchpoint-binding protein during the formation of the second commitment complex
S000001497	YKL014C	URB1	Unhealthy Ribosome Biogenesis	411619	416913	-1	Protein required for the normal accumulation of 25S and 5.8S rRNAs	Protein required for the normal accumulation of 25S and 5.8S rRNAs; nucleolar protein; associated with the 27SA2 pre-ribosomal particle; proposed to be involved in the biogenesis of the 60S ribosomal subunit
S000001498	YKL015W	PUT3	Proline UTilization	408544	411483	1	Transcriptional activator	Transcriptional activator; binds specific gene recruitment sequences and is required for DNA zip code-mediated targeting of genes to nuclear periphery; regulates proline utilization genes, constitutively binds PUT1 and PUT2 promoters as a dimer, undergoes conformational change to form active state; binds other promoters only under activating conditions; differentially phosphorylated in presence of different nitrogen sources; has a Zn(2)-Cys(6) binuclear cluster domain
S000001499	YKL016C	ATP7	ATP synthase	407465	407989	-1	Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase	Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis
S000001500	YKL017C	HCS1	dna HeliCaSe	405190	407241	-1	Hexameric DNA polymerase alpha-associated DNA helicase A	Hexameric DNA polymerase alpha-associated DNA helicase A; involved in lagging strand DNA synthesis; contains single-stranded DNA stimulated ATPase and dATPase activities; replication protein A stimulates helicase and ATPase activities
S000001502	YKL019W	RAM2	RAS protein and A-factor Maturation	402568	403518	1	Alpha subunit of farnesyltransferase and geranylgeranyltransferase-I	Alpha subunit of farnesyltransferase and geranylgeranyltransferase-I; farnesyltransferase (Ram2p-Ram1p heterodimer) catalyzes the addition of 15-carbon isoprenoid farnesyl to substrate proteins containing a CAAX consensus motif; type I geranylgeranyltransferase (Ram2p-Cdc43p heterodimer) catalyzes the addition of the 20-carbon isoprenoid geranylgeranyl to substrate proteins containing a CaaL consensus motif; required for membrane localization of Ras proteins and a-factor
S000001503	YKL020C	SPT23	SuPpressor of Ty	398832	402080	-1	ER membrane protein involved in regulation of OLE1 transcription	ER membrane protein involved in regulation of OLE1 transcription; inactive ER form dimerizes and one subunit is then activated by ubiquitin/proteasome-dependent processing followed by nuclear targeting; SPT23 has a paralog, MGA2, that arose from the whole genome duplication
S000001504	YKL021C	MAK11	MAintenance of Killer	397344	398750	-1	Protein involved in an early step of 60S ribosomal subunit biogenesis	Protein involved in an early step of 60S ribosomal subunit biogenesis; essential for cell growth and replication of killer M1 dsRNA virus; contains four beta-transducin repeats
S000001505	YKL022C	CDC16	Cell Division Cycle	394726	397248	-1	Subunit of the anaphase-promoting complex/cyclosome (APC/C)	Subunit of the anaphase-promoting complex/cyclosome (APC/C); which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; required for sporulation; relocalizes to the cytosol in response to hypoxia
S000001506	YKL023W	SKA1	SKi Associated factor	393721	394554	1	SKI complex-associated protein	SKI complex-associated protein; involved in 3'-5' degradation of long 3'UTR-containing mRNA, poorly translated mRNA, and other RNA regions devoid of ribosomes; proposed to assist the cytoplasmic exosome in a Ska1-SKI complex when a direct interaction between the ribosome and the SKI complex is absent
S000001508	YKL025C	PAN3	Poly(A) Nuclease	390240	392279	-1	Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex	Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex; poly (A) mRNA binding subunit which recruits mRNA to the complex; the Pan2p-Pan3p complex controls poly(A) tail length and regulates the stoichiometry and activity of postreplication repair complexes
S000001509	YKL026C	GPX1	Glutathione PeroXidase	389380	389883	-1	Phospholipid hydroperoxide glutathione peroxidase	Phospholipid hydroperoxide glutathione peroxidase; induced by glucose starvation that protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress; glutathione peroxidase activity is neuroprotective in models of Huntington's disease; GPX1 has a paralog, HYR1, that arose from the whole genome duplication
S000001510	YKL027W	TCD2	tRNA ThreonylCarbamoyladenosine Dehydratase	387562	388905	1	tRNA threonylcarbamoyladenosine dehydratase	tRNA threonylcarbamoyladenosine dehydratase; required for the ct6A tRNA base modification, where an adenosine at position 37 is modified to form a cyclized active ester with an oxazolone ring; localized to the mitochondrial outer membrane; TCD2 has a paralog, TCD1, that arose from the whole genome duplication
S000001511	YKL028W	TFA1	Transcription Factor A	385758	387206	1	TFIIE large subunit	TFIIE large subunit; involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening
S000001512	YKL029C	MAE1	MAlic Enzyme	382716	384725	-1	Mitochondrial malic enzyme	Mitochondrial malic enzyme; catalyzes the oxidative decarboxylation of malate to pyruvate, which is a key intermediate in sugar metabolism and a precursor for synthesis of several amino acids
S000001513	YKL030W	""	""	382498	383103	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; partially overlaps the verified gene MAE1
S000001514	YKL031W	""	""	382072	382485	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species
S000001515	YKL032C	IXR1	Intrastrand cross (X)-link Recognition	380065	381858	-1	Transcriptional repressor that regulates hypoxic genes during normoxia	Transcriptional repressor that regulates hypoxic genes during normoxia; involved in the aerobic repression of genes such as COX5b, TIR1, and HEM13; binds DNA intrastrand cross-links formed by cisplatin; HMG (high mobility group box) domain containing protein which binds and bends cisplatin-modified DNA, blocking excision repair; IXR1 has a paralog, ABF2, that arose from the whole genome duplication
S000001516	YKL033W	TTI1	Two Tel2-Interacting protein	375456	378572	1	Subunit of the ASTRA complex, involved in chromatin remodeling	Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1; similar to S. pombe Tti1p; detected in highly purified mitochondria in high-throughput studies
S000001517	YKL034W	TUL1	Transmembrane Ubiquitin Ligase	371829	374105	1	Subunit of the DSC ubiquitin ligase complex	Subunit of the DSC ubiquitin ligase complex; golgi-localized RING-finger ubiquitin ligase (E3) involved in sorting polar transmembrane domain containing membrane proteins to multivesicular bodies for delivery to the vacuole; proposed involvement in the quality control of misfolded TMD containing proteins; ortholog of fission yeast dsc1
S000001518	YKL035W	UGP1	UDP-glucose pyrophosphorylase	369891	371390	1	UDP-glucose pyrophosphorylase (UGPase)	UDP-glucose pyrophosphorylase (UGPase); catalyses the reversible formation of UDP-Glc from glucose 1-phosphate and UTP, involved in a wide variety of metabolic pathways, expression modulated by Pho85p through Pho4p; involved in PKA-mediated oxidative stress resistance and long-term survival in stationary phase; UGP1 has a paralog, YHL012W, that arose from the whole genome duplication
S000001520	YKL037W	AIM26	Altered Inheritance rate of Mitochondria	369365	369721	1	Protein of unknown function	Protein of unknown function; null mutant is viable and displays elevated frequency of mitochondrial genome loss; null mutation confers sensitivity to tunicamycin and DTT
S000001521	YKL038W	RGT1	Restores Glucose Transport	365605	369117	1	Glucose-responsive transcription factor	Glucose-responsive transcription factor; regulates expression of several glucose transporter (HXT) genes in response to glucose; binds to promoters and acts both as a transcriptional activator and repressor; recruits Tup1p/Cyc8p to target gene promoters; RGT1 has a paralog, EDS1, that arose from the whole genome duplication
S000001522	YKL039W	PTM1	""	362622	364193	1	Protein of unknown function	Protein of unknown function; copurifies with late Golgi vesicles containing the v-SNARE Tlg2p; PTM1 has a paralog, YHL017W, that arose from the whole genome duplication
S000001523	YKL040C	NFU1	NifU-like protein	361058	361828	-1	Protein involved in Fe-S cluster transfer to mitochondrial clients	Protein involved in Fe-S cluster transfer to mitochondrial clients; protects [4Fe-4S] clusters from damage due to oxidative stress; acts along with Bol3 at a late step in the transfer of [4Fe-4S] clusters from the ISA complex to client proteins; Fe-S loaded homodimer at steady state; similar to NifU, a bacterial protein required for Fe/S cluster maturation; ortholog of the human NFU1, mutations of which are associated with Multiple Mitochondria Dysfunctions Syndrome (MMDS1)
S000001524	YKL041W	VPS24	Vacuolar Protein Sorting	360143	360817	1	One of four subunits of the ESCRT-III complex	One of four subunits of the ESCRT-III complex; forms an endosomal sorting complex required for transport III (ESCRT-III) subcomplex with Did4p; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway
S000001525	YKL042W	SPC42	Spindle Pole Component	358475	359566	1	Central plaque component of spindle pole body (SPB)	Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane
S000001526	YKL043W	PHD1	PseudoHyphal Determinant	356748	357848	1	Transcriptional activator that enhances pseudohyphal growth	Transcriptional activator that enhances pseudohyphal growth; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; regulates expression of FLO11, an adhesin required for pseudohyphal filament formation; similar to StuA, an A. nidulans developmental regulator; potential Cdc28p substrate; PHD1 has a paralog, SOK2, that arose from the whole genome duplication
S000001527	YKL044W	MMO1	Mini Mitochondria ORF	356322	356642	1	Protein of unknown function	Protein of unknown function; SWAT-GFP, seamless-GFP and mCherry fusion proteins localize to the mitochondria; mRNA identified as translated by ribosome profiling data; MMO1 is a non-essential gene
S000001528	YKL045W	PRI2	DNA PRImase	353493	355079	1	Subunit of DNA primase	Subunit of DNA primase; DNA primase is required for DNA synthesis and double-strand break repair
S000001529	YKL046C	DCW1	Defective Cell Wall	351275	352624	-1	Putative mannosidase	Putative mannosidase; GPI-anchored membrane protein required for cell wall biosynthesis in bud formation;homologous to Dfg5p
S000001530	YKL047W	ANR2	Avl Nine Related family	349463	351013	1	Protein of unknown function	Protein of unknown function; may have a role in lipid metabolism, based on localization to lipid droplets; predicted to be palmitoylated
S000001531	YKL048C	ELM1	ELongated Morphology	347214	349136	-1	Serine/threonine protein kinase	Serine/threonine protein kinase; regulates the orientation checkpoint, the morphogenesis checkpoint and the metabolic switch from fermentative to oxidative metabolism by phosphorylating the activation loop of Kin4p, Hsl1p and Snf1p respectively; cooperates with Hsl7p in recruiting Hsl1p to the septin ring, a prerequisite for subsequent recruitment, phosphorylation, and degradation of Swe1p; forms part of the bud neck ring; regulates cytokinesis
S000001532	YKL049C	CSE4	Chromosome SEgregation	346075	346764	-1	Centromeric histone H3-like protein	Centromeric histone H3-like protein; associates with promoters, accessible chromatin, and RNAPII-bound regions; Cse4p associates with centromeres, promoting kinetochore assembly and Sgo1p association with centromeric chromatin; Ipl1p-dependent phosphorylation destabilizes defective kinetochores promoting bi-orientation; phosphorylation by Cdc7p maintains Cse4p at kinetochores and promotes faithful chromosome segregation; regulated by multiple E3 ligases; complements human homolog CENPA mutations
S000001533	YKL050C	LPX2	Lipase of PeroXisomes	342852	345620	-1	Peroxisomal matrix-localized lipase	Peroxisomal matrix-localized lipase; target of SCFCdc4 ubiquitin ligase complex; peroxisomal, nuclear and cytosolic localization is dependent on the presence of oleate; transcription is regulated by Azf1p; LPX2 has a paralog, EIS1, that arose from the whole genome duplication
S000001534	YKL051W	SFK1	Suppressor of Four Kinase	341312	342373	1	Protein that regulates phospholipid asymmetry at plasma membrane	Protein that regulates phospholipid asymmetry at plasma membrane; may act to generate normal levels of PI4P; may act together with or upstream of Stt4p; at least partially mediates proper localization of Stt4p to the plasma membrane
S000001535	YKL052C	ASK1	Associated with Spindles and Kinetochores	339668	340546	-1	Essential subunit of the Dam1 complex (aka DASH complex)	Essential subunit of the Dam1 complex (aka DASH complex); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kinases; sumoylated in an Mms21p-dependent manner; protein abundance increases in response to DNA replication stress
S000001536	YKL053W	""	""	339572	339946	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ASK1
S000001537	YKL054C	DEF1	RNAPII DEgradation Factor	336537	338753	-1	RNAPII degradation factor	RNAPII degradation factor; forms a complex with Rad26p in chromatin, enables ubiquitination and proteolysis of RNAPII present in an elongation complex; interacts with general transcription factor TFIIH; mutant is deficient in Zip1p loading onto chromosomes during meiosis; null mutant is prone to self-diploidization; null mutation can be functionally complemented by human UBAP2 and UBAP2
S000001538	YKL055C	OAR1	3-Oxoacyl-[Acyl-carrier-protein] Reductase	335321	336157	-1	Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase	Mitochondrial 3-oxoacyl-[acyl-carrier-protein] reductase; may comprise a type II mitochondrial fatty acid synthase along with Mct1p; human homolog CBR4 complements yeast null mutant
S000001539	YKL056C	TMA19	Translation Machinery Associated	334412	334915	-1	Protein that associates with ribosomes	Protein that associates with ribosomes; homolog of translationally controlled tumor protein; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and relocates to the mitochondrial outer surface upon oxidative stress; homolog of human TPT1
S000001540	YKL057C	NUP120	NUclear Pore	330856	333969	-1	Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC)	Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis and is involved in establishment of a normal nucleocytoplasmic concentration gradient of the GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP160
S000001542	YKL059C	MPE1	Mutant PCF11 Extragenic suppressor	328118	329443	-1	Subunit of CPF cleavage and polyadenylation factor and E3 Ub-ligase	Subunit of CPF cleavage and polyadenylation factor and E3 Ub-ligase; detects polyadenylation signal in nascent pre-mRNA and triggers 3' end formation of mRNA via cleavage and polyadenylation; binds pre-mRNA via zinc knuckle and RING finger; E3 ubiquitin ligase involved in polyubiquitination of endonuclease Ysh1p; contains a ubiquitin-like (UBL) domain, a RNA-binding zinc knuckle motif and a RING finger domain; relocalizes to cytosol in response to hypoxia; homolog of human RBBP6
S000001543	YKL060C	FBA1	Fructose-1,6-Bisphosphate Aldolase	326408	327487	-1	Fructose 1,6-bisphosphate aldolase	Fructose 1,6-bisphosphate aldolase; required for glycolysis and gluconeogenesis; catalyzes conversion of fructose 1,6 bisphosphate to glyceraldehyde-3-P and dihydroxyacetone-P; locates to mitochondrial outer surface upon oxidative stress; N-terminally propionylated in vivo
S000001544	YKL061W	BLI1	BLoc-1 Interactor	325771	326112	1	Subunit of the BLOC-1 complex involved in endosomal maturation	Subunit of the BLOC-1 complex involved in endosomal maturation; interacts with Msb3p; green fluorescent protein (GFP)-fusion protein localizes to the endosome
S000001545	YKL062W	MSN4	Multicopy suppressor of SNF1 mutation	323228	325120	1	Stress-responsive transcriptional activator	Stress-responsive transcriptional activator; activated in stochastic pulses of nuclear localization in response to various stress conditions; binds DNA at stress response elements of responsive genes, inducing gene expression; involved in diauxic shift
S000001546	YKL063C	""	""	321015	321518	-1	Putative protein of unknown function	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi
S000001547	YKL064W	MNR2	MaNganese Resistance	317764	320673	1	Vacuolar membrane protein required for magnesium homeostasis	Vacuolar membrane protein required for magnesium homeostasis; putative magnesium transporter; has similarity to Alr1p and Alr2p, which mediate influx of Mg2+ and other divalent cations; localizes to sites of contact between the vacuole and mitochondria (vCLAMPs)
S000001548	YKL065C	YET1	Yeast Endoplasmic reticulum Transmembrane protein	316437	317057	-1	Endoplasmic reticulum transmembrane protein	Endoplasmic reticulum transmembrane protein; may interact with ribosomes, based on co-purification experiments; homolog of human BAP31 protein; YET1 has a paralog, YET2, that arose from the whole genome duplication
S000001549	YKL066W	""	""	315281	315724	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; partially overlaps the verified gene YNK1
S000001550	YKL067W	YNK1	Yeast Nucleoside diphosphate Kinase	314812	315273	1	Nucleoside diphosphate kinase	Nucleoside diphosphate kinase; catalyzes the transfer of gamma phosphates from nucleoside triphosphates, usually ATP, to nucleoside diphosphates by a mechanism that involves formation of an autophosphorylated enzyme intermediate; protein abundance increases in response to DNA replication stress
S000001551	YKL068W	NUP100	NUclear Pore	310199	313078	1	FG-nucleoporin component of central core of the nuclear pore complex	FG-nucleoporin component of central core of the nuclear pore complex; contributes directly to nucleocytoplasmic transport and maintenance of the nuclear pore complex (NPC) permeability barrier and is involved in gene tethering at the nuclear periphery; NUP100 has a paralog, NUP116, that arose from the whole genome duplication
S000001552	YKL069W	""	""	307285	307827	1	Methionine-R-sulfoxide reductase	Methionine-R-sulfoxide reductase; reduces the R enantiomer of free Met-SO, in contrast to Ycl033Cp which reduces Met-R-SO in a peptide linkage; has a role in protection against oxidative stress; relative distribution to the nucleus increases upon DNA replication stress
S000001553	YKL070W	""	""	306211	306720	1	Putative protein of unknown function	Putative protein of unknown function; expression induced in cells treated with mycotoxins patulin or citrinin; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
S000001554	YKL071W	OSI1	Oxidative Stress Induced	305114	305884	1	NADH-dependent short-chain aldehyde reductase	NADH-dependent short-chain aldehyde reductase; involved in detoxification of furfural; expression is upregulated in cells treated with the aldehydes furfural and glycolaldehyde, the mycotoxin patulin, and also the quinone methide triterpene celastrol; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
S000001555	YKL072W	STB6	Sin Three Binding protein	299582	301882	1	Protein that binds Sin3p in a two-hybrid assay	Protein that binds Sin3p in a two-hybrid assay; STB6 has a paralog, STB2, that arose from the whole genome duplication
S000001556	YKL073W	LHS1	Lumenal Hsp Seventy	296430	299075	1	Molecular chaperone of the endoplasmic reticulum lumen	Molecular chaperone of the endoplasmic reticulum lumen; involved in polypeptide translocation and folding; nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; regulated by the unfolded protein response pathway
S000001557	YKL074C	MUD2	Mutant U1 Die	294610	296193	-1	Protein involved in early pre-mRNA splicing	Protein involved in early pre-mRNA splicing; component of the pre-mRNA-U1 snRNP complex, the commitment complex; interacts with Msl5p/BBP splicing factor and Sub2p; similar to metazoan splicing factor U2AF65
S000001558	YKL075C	AAN1	Actin, Aging and Nutrient modulator	292956	294308	-1	Protein of unknown function	Protein of unknown function; regulates actin cable stability and branched-chain amino acid (BCAA) metabolism; involved in mitochondrial quality control and longevity; proposed to be involved in resistance to streptozotocin and camptothecin; localizes to punctate cytosolic structures 
S000001559	YKL076C	PSY1	Platinum SensitivitY	292838	293221	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C
S000001560	YKL077W	PSG1	Pma1 Stabilization in the Golgi	291453	292631	1	Protein that promotes the transport and maturation of Pma1 with Exp1	Protein that promotes the transport and maturation of Pma1 with Exp1; cleaved specifically by Kex2p; localizes to the Golgi apparatus and to the endoplasmic reticulum in HTP studies
S000001561	YKL078W	DHR2	DEAH-box RNA helicase	288845	291052	1	Predominantly nucleolar DEAH-box ATP-dependent RNA helicase	Predominantly nucleolar DEAH-box ATP-dependent RNA helicase; required for 18S rRNA synthesis
S000001562	YKL079W	SMY1	Suppressor of MYo2-66	286603	288573	1	Kinesin-like myosin passenger-protein	Kinesin-like myosin passenger-protein; interacts with Myo2p and enhances its interaction with Sec4p during transport of secretory vesicles; controls actin cable structure and dynamics
S000001563	YKL080W	VMA5	Vacuolar Membrane Atpase	285030	286208	1	Subunit C of the V1 peripheral membrane domain of V-ATPase	Subunit C of the V1 peripheral membrane domain of V-ATPase; part of the electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane; the V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase) has eight subunits
S000001564	YKL081W	TEF4	Translation Elongation Factor	282891	284455	1	Gamma subunit of translational elongation factor eEF1B	Gamma subunit of translational elongation factor eEF1B; stimulates the binding of aminoacyl-tRNA (AA-tRNA) to ribosomes by releasing eEF1A (Tef1p/Tef2p) from the ribosomal complex
S000001565	YKL082C	RRP14	Ribosomal RNA Processing	281025	282329	-1	Essential protein, constituent of 66S pre-ribosomal particles	Essential protein, constituent of 66S pre-ribosomal particles; interacts with proteins involved in ribosomal biogenesis and cell polarity; member of the SURF-6 family
S000001566	YKL083W	""	""	280921	281535	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; partially overlaps the verified essential gene RRP14
S000001567	YKL084W	HOT13	Helper Of Tim	280509	280859	1	Zinc-binding mitochondrial intermembrane space (IMS) protein	Zinc-binding mitochondrial intermembrane space (IMS) protein; involved in a disulfide relay system for IMS import of cysteine-containing proteins; binds Mia40p and stimulates its Erv1p-dependent oxidation, probably by sequestering zinc
S000001568	YKL085W	MDH1	Malate DeHydrogenase	279123	280127	1	Mitochondrial malate dehydrogenase	Mitochondrial malate dehydrogenase; catalyzes interconversion of malate and oxaloacetate; involved in the tricarboxylic acid (TCA) cycle; phosphorylated; mutation in human homolog MDH2 causes early-onset severe encephalopathy
S000001569	YKL086W	SRX1	SulfiRedoXin	278281	278664	1	Sulfiredoxin	Sulfiredoxin; contributes to oxidative stress resistance by reducing cysteine-sulfinic acid groups in the peroxiredoxin Tsa1p, which is formed upon exposure to oxidants; conserved in higher eukaryotes; protein abundance increases in response to DNA replication stress
S000001570	YKL087C	CYT2	CYTochrome-c1-heme-lyase	277189	277863	-1	Cytochrome c1 heme lyase	Cytochrome c1 heme lyase; involved in maturation of cytochrome c1, which is a subunit of the mitochondrial ubiquinol-cytochrome-c reductase; links heme covalently to apocytochrome c1; human homolog HCCS can complement yeast cyt2 null mutant
S000001571	YKL088W	CAB3	Coenzyme A Biosynthesis	275283	276998	1	Subunit of PPCDC and CoA-SPC complexes involved in CoA biosynthesis	Subunit of PPCDC and CoA-SPC complexes involved in CoA biosynthesis; subunits of the phosphopantothenoylcysteine decarboxylase (PPCDC) complex are: Cab3p, Sis2p, Vhs3p, while the subunits of the CoA-synthesizing protein complex (CoA-SPC) are: Cab2p, Cab3p, Cab4p, and Cab5p as well as Sis2p and Vhs3p; null mutant lethality is complemented by E. coli coaBC
S000001573	YKL090W	CUE2	Coupling of Ubiquitin conjugation to ER degradation	271878	273209	1	Ubiquitin-binding endonuclease involved in mRNA decay	Ubiquitin-binding endonuclease involved in mRNA decay; recruited to stalled ribosomes to promote breakdown of transcript body of nuclear-transcribed mRNA with stalls in translation elongation; cleaves mRNA within A site of colliding ribosome; has two CUE domains that bind ubiquitin, which may facilitate intramolecular monoubiquitination
S000001574	YKL091C	""	""	269718	270650	-1	Putative phosphatidylinositol/phosphatidylcholine transfer protein	Putative phosphatidylinositol/phosphatidylcholine transfer protein; possibly involved in lipid metabolism; localizes to the nucleus; contains a CRAL/TRIO domain and binds several lipids in a large-scale study; YKL091C has a paralog, SEC14, that arose from the whole genome duplication
S000001575	YKL092C	BUD2	BUD site selection	266145	269459	-1	GTPase activating factor for Rsr1p/Bud1p	GTPase activating factor for Rsr1p/Bud1p; plays a role in spindle position checkpoint distinct from its role in bud site selection; required for both axial and bipolar budding patterns; mutants exhibit random budding in all cell types; contains two PH-like domains
S000001576	YKL093W	MBR1	Mitochondrial Biogenesis Regulation	264789	265808	1	Protein involved in mitochondrial functions and stress response	Protein involved in mitochondrial functions and stress response; overexpression suppresses growth defects of hap2, hap3, and hap4 mutants; MBR1 has a paralog, ISF1, that arose from the whole genome duplication
S000001577	YKL094W	YJU3	""	263349	264290	1	Monoglyceride lipase (MGL) that hydrolyzes fatty acid ethyl esters	Monoglyceride lipase (MGL) that hydrolyzes fatty acid ethyl esters; required for removal of toxic metabolites in ethanol producing yeast cells; localizes to lipid particles and membranes; member of the eukaryotic serine hydrolase family; functional ortholog of mammalian MGL
S000001578	YKL095W	YJU2	""	262277	263113	1	Essential protein required for pre-mRNA splicing	Essential protein required for pre-mRNA splicing; associates transiently with the spliceosomal NTC ('nineteen complex') and acts after Prp2p to promote the first catalytic reaction of splicing
S000001579	YKL096W	CWP1	Cell Wall Protein	261132	261851	1	Cell wall mannoprotein that localizes to birth scars of daughter cells	Cell wall mannoprotein that localizes to birth scars of daughter cells; linked to beta-1,3- and beta-1,6-glucan heteropolymer through phosphodiester bond; required for propionic acid resistance; targeted to vacuole via AP-3 pathway
S000001580	YKL097C	""	""	258503	258913	-1	Putative protein of unknown function	Putative protein of unknown function; conserved across S. cerevisiae strains; not conserved in closely related Saccharomyces species
S000001581	YKL098W	MTC2	Maintenance of Telomere Capping	256770	257843	1	Protein of unknown function	Protein of unknown function; mtc2 is synthetically sick with cdc13-1
S000001583	YKL100C	YPF1	Yeast Presenilin-like Family	253697	255460	-1	Intramembrane aspartyl protease of the perinuclear ER membrane	Intramembrane aspartyl protease of the perinuclear ER membrane; acts in a branch of ER-associated degradation (ERAD) that degrades functional proteins rather than misfolded proteins; regulates abundance of high-affinity plasma membrane transporters, such as Ctr1p and Zrt1p, during the starvation response; has a presenilin fold; member of the GxGD family of intramembrane proteases; closest human homolog is signal peptide peptidase (SPP)
S000001584	YKL101W	HSL1	Histone Synthetic Lethal	248920	253476	1	Nim1p-related protein kinase	Nim1p-related protein kinase; septin-binding kinase that localizes to the bud neck septin ring and regulates the morphogenesis checkpoint; phosphorylates Hsl7p and cooperates with Elm1p to recruit Hsl7p to the mother-bud neck, as a prerequisite for the subsequent recruitment, phosphorylation, and degradation of Swe1p; autophosphorylation enhances interactions with Hsl7p
S000001585	YKL102C	""	""	248062	248367	-1	Putative protein of unknown function	Putative protein of unknown function; conserved across S. cerevisiae strains; deletion confers sensitivity to citric acid; predicted protein would include a thiol-disulfide oxidoreductase active site
S000001586	YKL103C	APE1	AminoPeptidase	246138	247682	-1	Vacuolar aminopeptidase yscI	Vacuolar aminopeptidase yscI; zinc metalloproteinase that belongs to peptidase family M18; often used as marker in studies of autophagy and cytosol to vacuole targeting (CVT) pathway; protein increases in abundance and relative distribution to cytoplasmic foci increases upon DNA replication stress; targeted to vacuole via Vps10p-dependent endosomal vacuolar protein sorting pathway and via CVT pathway
S000001588	YKL105C	SEG2	Stability of Eisosomes Guaranteed	239185	242583	-1	Eisosome component	Eisosome component; likely plays only a minor role in eisosome assembly; shown to interact with Seg1p by affinity purification and mass spec; SWAT-GFP and mCherry fusion proteins localize to the cell periphery; similar to <i>A. gossypii</i> SEG gene which is important for stabilizing eisosomes; SEG2 has a paralog, SEG1, that arose from the whole genome duplication
S000001589	YKL106W	AAT1	Aspartate AminoTransferase	237536	238891	1	Mitochondrial aspartate aminotransferase	Mitochondrial aspartate aminotransferase; catalyzes the conversion of oxaloacetate to aspartate in aspartate and asparagine biosynthesis
S000001590	YKL107W	""	""	236141	237070	1	NADH-dependent aldehyde reductase	NADH-dependent aldehyde reductase; involved in the detoxification of acetaldehyde, glycolaldehyde, furfural, formaldehyde and propionaldehyde; localizes to the endoplasmic reticulum; member of the classical short-chain dehydrogenase/reductase (SDR) family
S000001591	YKL108W	SLD2	Synthetically Lethal with Dpb11-1	234426	235787	1	Single-stranded DNA origin-binding and annealing protein	Single-stranded DNA origin-binding and annealing protein; required for initiation of DNA replication; phosphorylated in S phase by cyclin-dependent kinases (Cdks), promoting origin binding, DNA replication and Dpb11p complex formation; component of the preloading complex; binds the Mcm2-7p complex to prevent inappropriate Mcm2-7p interaction with the GINS complex in G1; required for S phase checkpoint; relative distribution to the nucleus increases upon DNA replication stress
S000001592	YKL109W	HAP4	Heme Activator Protein	232227	233891	1	Transcription factor	Transcription factor; subunit of the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex, a transcriptional activator and global regulator of respiratory gene expression; provides the principal activation function of the complex; involved in diauxic shift
S000001593	YKL110C	KTI12	Kluveromyces lactis Toxin Insensitive	228939	229880	-1	Protein that plays a role in modification of tRNA wobble nucleosides	Protein that plays a role in modification of tRNA wobble nucleosides; protein plays role in tRNA wobble nucleoside modification with Elongator complex; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p
S000001595	YKL112W	ABF1	ARS-Binding Factor	226570	228765	1	DNA binding protein with possible chromatin-reorganizing activity	DNA binding protein with possible chromatin-reorganizing activity; involved in transcriptional activation, gene silencing, and DNA replication and repair
S000001596	YKL113C	RAD27	RADiation sensitive	224727	225875	-1	5' to 3' exonuclease, 5' flap endonuclease	5' to 3' exonuclease, 5' flap endonuclease; required for Okazaki fragment processing and maturation, for long-patch base-excision repair and large loop repair (LLR), ribonucleotide excision repair; cleaves off RNA flap in a RNA:DNA hybrid intermediate during processing of DNA lagging strand; relocalizes to the cytosol in response to hypoxia; member of the S. pombe RAD2/FEN1 family
S000001597	YKL114C	APN1	APurinic/apyrimidinic eNdonuclease	223352	224455	-1	Major apurinic/apyrimidinic endonuclease	Major apurinic/apyrimidinic endonuclease; 3'-repair diesterase; involved in repair of DNA damage by oxidation and alkylating agents; also functions as a 3'-5' exonuclease to repair 7,8-dihydro-8-oxodeoxyguanosine; genetically interacts with NTG1 to maintain mitochondrial genome integrity
S000001598	YKL115C	""	""	222893	223285	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PRR1
S000001599	YKL116C	PRR1	Pheromone Response Regulator	221344	222900	-1	Serine/threonine protein kinase	Serine/threonine protein kinase; inhibits pheromone induced signaling downstream of MAPK, possibly at the level of the Ste12p transcription factor
S000001600	YKL117W	SBA1	increased Sensitivity to Benzoquinone Ansamycins	220324	220974	1	Co-chaperone that binds and regulates Hsp90 family chaperones	Co-chaperone that binds and regulates Hsp90 family chaperones; plays a role in determining prion variants; important for pp60v-src activity in yeast; homologous to the mammalian p23 proteins, and like p23 can regulate telomerase activity; protein abundance increases in response to DNA replication stress
S000001601	YKL118W	""	""	219126	219437	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPH2
S000001602	YKL119C	VPH2	Vacuolar pH	218570	219217	-1	Integral membrane protein required for V-ATPase function	Integral membrane protein required for V-ATPase function; not an actual component of the vacuolar H+-ATPase (V-ATPase) complex; functions in the assembly of the V-ATPase; localized to the endoplasmic reticulum (ER); involved in methionine restriction extension of chronological lifespan in an autophagy-dependent manner
S000001603	YKL120W	OAC1	OxaloAcetate Carrier	217344	218318	1	Mitochondrial inner membrane transporter	Mitochondrial inner membrane transporter; transports oxaloacetate, sulfate, thiosulfate, and isopropylmalate; member of the mitochondrial carrier family
S000001604	YKL121W	DGR2	2-Deoxy-Glucose Resistant	214142	216700	1	Protein of unknown function	Protein of unknown function; null mutant is resistant to 2-deoxy-D-glucose and displays abnormally elongated buds; DGR2 has a paralog, YMR102C, that arose from the whole genome duplication
S000001605	YKL122C	SRP21	Signal Recognition Particle	212495	212998	-1	Subunit of the signal recognition particle (SRP)	Subunit of the signal recognition particle (SRP); SRP functions in protein targeting to the endoplasmic reticulum membrane; not found in mammalian SRP; forms a pre-SRP structure in the nucleolus that is translocated to the cytoplasm
S000001606	YKL123W	""	""	212043	212423	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SSH4
S000001607	YKL124W	SSH4	Suppressor of SHr3 deletion	210593	212332	1	Specificity factor required for Rsp5p-dependent ubiquitination	Specificity factor required for Rsp5p-dependent ubiquitination; also required for sorting of cargo proteins at the multivesicular body; identified as a high-copy suppressor of a SHR3 deletion, increasing steady-state levels of amino acid permeases
S000001608	YKL125W	RRN3	Regulation of RNA polymerase I	208247	210130	1	Protein required for transcription of rDNA by RNA polymerase I	Protein required for transcription of rDNA by RNA polymerase I; transcription factor independent of DNA template; involved in recruitment of RNA polymerase I to rDNA; structure reveals unique HEAT repeat fold and a surface serine patch; phosphorylation of serine patch impairs cell growth and reduces RNA polymerase I binding in vitro and RNA polymerase I recruitment to the rDNA gene in vivo
S000001609	YKL126W	YPK1	Yeast Protein Kinase	205707	207749	1	S/T protein kinase	S/T protein kinase; required for PM lipid and protein homeostasis; involved in sphingolipid-mediated and cell integrity signaling pathways and endocytosis; phosphorylates and downregulates Fpk1p; phosphorylates and inactivates Orm1p/2p when sphingolipid synthesis is compromised; role in TORC1/2-dependent Rps6a/b phosphorylation; phosphorylated and activated by TORC2 and by Pkh1/2p in the presence of phytosphingosine; human homolog SGK1 complements the null and inviability of a ypk1 ypk2 mutant
S000001610	YKL127W	PGM1	PhosphoGlucoMutase	203541	205253	1	Phosphoglucomutase, minor isoform	Phosphoglucomutase, minor isoform; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism; PGM1 has a paralog, PGM2, that arose from the whole genome duplication
S000001611	YKL128C	PMU1	PhosphoMUtase	200884	201771	-1	Putative phosphomutase	Putative phosphomutase; contains a region homologous to the active site of phosphomutases; overexpression suppresses the histidine auxotrophy of an ade3 ade16 ade17 triple mutant and the temperature sensitivity of a tps2 mutant; localizes to cytosol an peroxisomes
S000001612	YKL129C	MYO3	MYOsin	196349	200167	-1	One of two type I myosins	One of two type I myosins; localizes to actin cortical patches; deletion of MYO3 has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization; MYO3 has a paralog, MYO5, that arose from the whole genome duplication
S000001613	YKL130C	SHE2	Swi5p-dependent HO Expression	195288	196028	-1	RNA-binding protein that binds specific mRNAs and interacts with She3p	RNA-binding protein that binds specific mRNAs and interacts with She3p; part of the mRNA localization machinery that restricts accumulation of certain proteins to the bud; binds to ER-derived membranes and targets mRNAs to cortical ER
S000001614	YKL131W	""	""	194478	194999	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000001615	YKL132C	RMA1	Reduced Mating A	193574	194866	-1	Putative dihydrofolate synthetase	Putative dihydrofolate synthetase; similar to E. coli folylpolyglutamate synthetase/dihydrofolate synthetase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; RMA1 has a paralog, FOL3, that arose from the whole genome duplication
S000001616	YKL133C	RCI50	Respiratory Chain Interacting protein of ~50 kDa	191679	193070	-1	Mitochondrial protein of unknown function	Mitochondrial protein of unknown function; not required for growth of cells lacking the mitochondrial genome; SWAT-GFP and mCherry fusion proteins localize to the mitochondria; YKL133C has a paralog, MGR3, that arose from the whole genome duplication
S000001617	YKL134C	OCT1	OCTapeptidyl aminopeptidase	189124	191442	-1	Mitochondrial intermediate peptidase	Mitochondrial intermediate peptidase; cleaves destabilizing N-terminal residues of a subset of proteins upon import, after their cleavage by mitochondrial processing peptidase (Mas1p-Mas2p); may contribute to mitochondrial iron homeostasis
S000001618	YKL135C	APL2	clathrin Adaptor Protein complex Large chain	186460	188640	-1	Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex	Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress
S000001619	YKL136W	""	""	186416	186814	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF APL2/YKL135C
S000001620	YKL137W	CMC1	Cx9C Mitochondrial protein necessary for full assembly of Cytochrome c oxidase	185957	186292	1	Copper-binding protein of the mitochondrial intermembrane space	Copper-binding protein of the mitochondrial intermembrane space; evolutionarily conserved; may be involved in delivering copper from the matrix to the cytochrome c oxidase complex; contains a twin CX9C motif
S000001621	YKL138C	MRPL31	Mitochondrial Ribosomal Protein, Large subunit	185286	185681	-1	Mitochondrial ribosomal protein of the large subunit	Mitochondrial ribosomal protein of the large subunit
S000001622	YKL139W	CTK1	Carboxy-Terminal domain Kinase	182958	184544	1	Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I)	Catalytic (alpha) subunit of C-terminal domain kinase I (CTDK-I); phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; required for H3K36 trimethylation but not dimethylation by Set2p; suggested stimulatory role in 80S formation during translation initiation; similar to the Drosophila dCDK12 and human CDK12 and probably CDK13
S000001623	YKL140W	TGL1	TriGlyceride Lipase	180779	182425	1	Steryl ester hydrolase	Steryl ester hydrolase; one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes
S000001624	YKL141W	SDH3	Succinate DeHydrogenase	179667	180263	1	Subunit of succinate dehydrogenase and of TIM22 translocase	Subunit of succinate dehydrogenase and of TIM22 translocase; functions as cytochrome b subunit of succinate dehydrogenase, which couples oxidation of succinate to transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; also required for mitochondrial inner membrane protein import as part of the TIM22 complex; SDH3 has a paralog, SHH3, that arose from the whole genome duplication
S000001625	YKL142W	MRP8	Mitochondrial Ribosomal Protein	178515	179174	1	Protein of unknown function	Protein of unknown function; undergoes sumoylation; transcription induced under cell wall stress; protein levels are reduced under anaerobic conditions; protein abundance increases in response to DNA replication stress; originally thought to be a mitochondrial ribosomal protein based on sequence analysis
S000001626	YKL143W	LTV1	Low Temperature Viability	176781	178172	1	Subunit of the EGO/GSE complex	Subunit of the EGO/GSE complex; the vacuolar/endosomal membrane associated EGO/GSE complex regulates exit from rapamycin-induced growth arrest, stimulating microautophagy and sorting of Gap1p from the endosome to the plasma membrane; required for ribosomal small subunit export from nucleus; required for growth at low temperature
S000001627	YKL144C	RPC25	RNA Polymerase C	175843	176481	-1	RNA polymerase III subunit C25	RNA polymerase III subunit C25; required for transcription initiation; forms a heterodimer with Rpc17p; paralog of Rpb7p
S000001628	YKL145W	RPT1	Regulatory Particle Triple-A protein, or Regulatory Particle Triphosphatase	174213	175616	1	ATPase of the 19S regulatory particle of the 26S proteasome	ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance
S000001629	YKL146W	AVT3	Amino acid Vacuolar Transport	171783	173861	1	Vacuolar transporter	Vacuolar transporter; exports large neutral amino acids from vacuole; targeted to vacuole via AP-3 pathway; member of family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
S000001630	YKL147C	""	""	171551	172168	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; partially overlaps the verified gene AVT3
S000001631	YKL148C	SDH1	Succinate DeHydrogenase	169207	171129	-1	Flavoprotein subunit of succinate dehydrogenase	Flavoprotein subunit of succinate dehydrogenase; couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; FAD binding to Sdh1p is required for the assembly of the succinate dehydrogenase subunits; mutations in human ortholog SDHA are associated with Leigh syndrome and predisposition to several cancers
S000001632	YKL149C	DBR1	DeBRanching	167612	168829	-1	RNA lariat debranching enzyme	RNA lariat debranching enzyme; catalyzes debranching of lariat introns formed during pre-mRNA splicing; required for efficient Ty1 transposition; knockdown of human homolog Dbr1 rescues toxicity of RNA-binding proteins TDP-43 and FUS which are implicated in amyotrophic lateral sclerosis (ALS), suggests potential therapeutic target for ALS and related TDP-43 proteinopathies; human homolog DBR1 can complement yeast dbr1 null mutant
S000001633	YKL150W	MCR1	Mitochondrial NADH-Cytochrome b5 Reductase	166544	167452	1	Mitochondrial NADH-cytochrome b5 reductase	Mitochondrial NADH-cytochrome b5 reductase; involved in ergosterol biosynthesis
S000001634	YKL151C	NNR2	Nicotinamide Nucleotide Repair	164917	165930	-1	Widely-conserved NADHX dehydratase	Widely-conserved NADHX dehydratase; converts (S)-NADHX to NADH in ATP-dependent manner; YKL151C promoter contains STREs (stress response elements) and expression is induced by heat shock or methyl methanesulfonate; downstream intergenic region drives antisense expression and mediates coordinated regulation of YKL151C and GPM1 phosphoglycerate mutase; protein abundance increases in response to DNA replication stress; homolog of Carkd in mammals and C-terminus of YjeF in E.coli
S000001637	YKL154W	SRP102	Signal Recognition Particle	161601	162335	1	Signal recognition particle (SRP) receptor beta subunit	Signal recognition particle (SRP) receptor beta subunit; involved in SRP-dependent protein targeting; anchors the alpha subunit, Srp101p to the ER membrane
S000001638	YKL155C	RSM22	Ribosomal Small subunit of Mitochondria	159456	161342	-1	Mitochondrial ribosomal protein of the small subunit	Mitochondrial ribosomal protein of the small subunit; also predicted to be an S-adenosylmethionine-dependent RNA methyltransferase
S000001639	YKL156W	RPS27A	Ribosomal Protein of the Small subunit	158613	159212	1	Protein component of the small (40S) ribosomal subunit	Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27A has a paralog, RPS27B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
S000001640	YKL157W	APE2	AminoPEptidase	154991	158232	1	Aminopeptidase yscII	Aminopeptidase yscII; may have role in obtaining leucine from dipeptide substrates; targeted to vacuole via Vps10p-dependent endosomal vacuolar protein sorting pathway; APE2 has a paralog, AAP1, that arose from the whole genome duplication
S000001642	YKL159C	RCN1	Regulator of CalciNeurin	153816	154451	-1	Protein involved in calcineurin regulation during calcium signaling	Protein involved in calcineurin regulation during calcium signaling; has similarity to H. sapiens DSCR1 which is found in the Down Syndrome candidate region
S000001643	YKL160W	ELF1	ELongation Factor	153269	153706	1	Transcription elongation factor with a conserved zinc finger domain	Transcription elongation factor with a conserved zinc finger domain; implicated in the maintenance of proper chromatin structure in actively transcribed regions; deletion inhibits Brome mosaic virus (BMV) gene expression
S000001644	YKL161C	KDX1	Kinase Dead X-talker	149386	150687	-1	Protein kinase	Protein kinase; implicated in Slt2p mitogen-activated (MAP) kinase signaling pathway; interacts with numerous components in the mating pheromone and CWI MAPK pathways; associates with Rlm1p; KDX1 has a paralog, SLT2, that arose from the whole genome duplication
S000001645	YKL162C	""	""	147630	148838	-1	Putative protein of unknown function	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion
S000001646	YKL163W	PIR3	Protein containing Internal Repeats	144401	145378	1	O-glycosylated covalently-bound cell wall protein	O-glycosylated covalently-bound cell wall protein; required for cell wall stability; expression is cell cycle regulated, peaking in M/G1 and also subject to regulation by the cell integrity pathway; coding sequence contains length polymorphisms in different strains; PIR3 has a paralog, HSP150, that arose from the whole genome duplication
S000001647	YKL164C	PIR1	Protein containing Internal Repeats	141794	142819	-1	O-glycosylated protein required for cell wall stability	O-glycosylated protein required for cell wall stability; attached to the cell wall via beta-1,3-glucan; mediates mitochondrial translocation of Apn1p; expression regulated by the cell integrity pathway and by Swi5p during the cell cycle; PIR1 has a paralog, YJL160C, that arose from the whole genome duplication
S000001648	YKL165C	MCD4	Morphogenesis Checkpoint Dependent	137932	140691	-1	Protein involved in GPI anchor synthesis	Protein involved in GPI anchor synthesis; multimembrane-spanning protein that localizes to the endoplasmic reticulum; highly conserved among eukaryotes; GPI stands for glycosylphosphatidylinositol
S000001649	YKL166C	TPK3	Takashi's Protein Kinase	134509	135705	-1	cAMP-dependent protein kinase catalytic subunit	cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; partially redundant with Tpk1p and Tpk2p; localizes to P-bodies during stationary phase; TPK3 has a paralog, TPK1, that arose from the whole genome duplication
S000001650	YKL167C	MRP49	Mitochondrial Ribosomal Protein	133721	134134	-1	Mitochondrial ribosomal protein of the large subunit	Mitochondrial ribosomal protein of the large subunit; not essential for mitochondrial translation
S000001651	YKL168C	KKQ8	""	131288	133462	-1	Putative serine/threonine protein kinase with unknown cellular role	Putative serine/threonine protein kinase with unknown cellular role; KKQ8 has a paralog, HAL5, that arose from the whole genome duplication
S000001652	YKL169C	""	""	130685	131068	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MRPL38
S000001653	YKL170W	MRPL38	Mitochondrial Ribosomal Protein, Large subunit	130634	131050	1	Mitochondrial ribosomal protein of the large subunit	Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL34 and YmL38) on two-dimensional SDS gels; protein abundance increases in response to DNA replication stress
S000001654	YKL171W	NNK1	Nitrogen Network Kinase	127475	130261	1	Protein kinase	Protein kinase; implicated in proteasome function; interacts with TORC1, Ure2 and Gdh2; overexpression leads to hypersensitivity to rapamycin and nuclear accumulation of Gln3; epitope-tagged protein localizes to the cytoplasm
S000001656	YKL173W	SNU114	Small NUclear ribonucleoprotein associated	122517	125543	1	GTPase component of U5 snRNP involved in mRNA splicing via spliceosome	GTPase component of U5 snRNP involved in mRNA splicing via spliceosome; binds directly to U5 snRNA; proposed to be involved in conformational changes of the spliceosome; similarity to ribosomal translocation factor EF-2
S000001657	YKL174C	TPO5	Transporter of POlyamines	120380	122236	-1	Protein involved in excretion of putrescine and spermidine	Protein involved in excretion of putrescine and spermidine; putative polyamine transporter in the Golgi or post-Golgi vesicles
S000001658	YKL175W	ZRT3	Zinc-Regulated Transporter	118793	120304	1	Vacuolar membrane zinc transporter	Vacuolar membrane zinc transporter; transports zinc from storage in vacuole to cytoplasm when needed; transcription induced under conditions of zinc deficiency; targeted to vacuole via AP-3 pathway
S000001659	YKL176C	LST4	Lethal with Sec Thirteen	115500	117986	-1	Subunit of the Lst4p-Lst7p GTPase activating protein complex for Gtr2p	Subunit of the Lst4p-Lst7p GTPase activating protein complex for Gtr2p; stimulates the GTPase activity of Rag family GTPase Gtr2p, within the context of the Gtr1p-Gtr2p heterodimer, after amino acid stimulation; required for activation of TORC1 in response to amino acid stimulation; recruited to the vacuolar membrane during amino acid starvation and released from the membrane by TORC1; required for the transport of amino acid permease Gap1p from the Golgi to the cell surface
S000001660	YKL177W	""	""	114356	114694	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene STE3
S000001661	YKL178C	STE3	STErile	113215	114627	-1	Receptor for a factor pheromone	Receptor for a factor pheromone; couples to MAP kinase cascade to mediate pheromone response; transcribed in alpha cells and required for mating by alpha cells, ligand bound receptors endocytosed and recycled to the plasma membrane; GPCR
S000001662	YKL179C	COY1	CASP Of Yeast	110464	112503	-1	Golgi membrane protein with similarity to mammalian CASP	Golgi membrane protein with similarity to mammalian CASP; packaged into COPII vesicles for trafficking between ER and Golgi; genetic interactions with GOS1 (encoding a Golgi snare protein) suggest a role in Golgi function
S000001665	YKL182W	FAS1	Fatty Acid Synthetase	100671	106826	1	Beta subunit of fatty acid synthetase	Beta subunit of fatty acid synthetase; complex catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities; specific target of the broad-spectrum antifungal NPD6433
S000001666	YKL183W	LOT5	LOw Temperature-responsive	98721	99641	1	Protein of unknown function	Protein of unknown function; gene expression increases in cultures shifted to a lower temperature; protein abundance increases in response to DNA replication stress
S000001667	YKL184W	SPE1	SPErmidine auxotroph	96757	98157	1	Ornithine decarboxylase	Ornithine decarboxylase; catalyzes the first step in polyamine biosynthesis; degraded in a proteasome-dependent manner in the presence of excess polyamines; deletion decreases lifespan, and increases necrotic cell death and ROS generation
S000001668	YKL185W	ASH1	Asymmetric Synthesis of HO	94499	96265	1	Component of the Rpd3L histone deacetylase complex	Component of the Rpd3L histone deacetylase complex; zinc-finger inhibitor of HO transcription; mRNA is localized and translated in the distal tip of anaphase cells, resulting in accumulation of Ash1p in daughter cell nuclei and inhibition of HO expression; potential Cdc28p substrate
S000001669	YKL186C	MTR2	Mrna TRansport	92744	93298	-1	mRNA transport regulator	mRNA transport regulator; essential nuclear protein; Mex67p and Mtr2p form a mRNA export complex which binds to RNA
S000001670	YKL187C	FAT3	FATty acid transporter	89284	91536	-1	Protein required for fatty acid uptake	Protein required for fatty acid uptake; protein abundance increases in cortical patches in response to oleate exposure; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; FAT3 has a paralog, YLR413W, that arose from the whole genome duplication
S000001671	YKL188C	PXA2	PeroXisomal ABC-transporter	86225	88786	-1	Subunit of heterodimeric peroxisomal ABC transport complex, with Pxa1p	Subunit of heterodimeric peroxisomal ABC transport complex, with Pxa1p; required for import of long-chain fatty acids into peroxisomes; similar to human adrenoleukodystrophy transportesr ABCD1 and ABCD2, and ALD-related proteins; mutations in ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), a peroxisomal disorder; human ABCD1 and ABCD2 can each partially complement yeast pxa1 pxa2 double null mutant
S000001672	YKL189W	HYM1	""	84704	85903	1	Component of the RAM signaling network	Component of the RAM signaling network; is involved in regulation of Ace2p activity and cellular morphogenesis; interacts with Sog2p; allosterically activates Kic1p; localizes to sites of polarized growth during budding and during the mating response
S000001673	YKL190W	CNB1	CalciNeurin subunit B	82947	83550	1	Calcineurin B	Calcineurin B; regulatory subunit of calcineurin, a Ca++/calmodulin-regulated type 2B protein phosphatase which regulates Crz1p (stress-response transcription factor); other calcineurin subunit encoded by CNA1 and/or CMP1; regulates function of Aly1p alpha-arrestin; myristoylation by Nmt1p reduces calcineurin activity in response to submaximal Ca signals, is needed to prevent constitutive phosphatase activity; protein abundance increases in response to DNA replication stress
S000001674	YKL191W	DPH2	DiPHthamide	81035	82639	1	Protein required for synthesis of diphthamide	Protein required for synthesis of diphthamide; required along with Dph1p, Kti11p, Jjj3p, and Dph5p; diphthamide is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); forms a complex with Dph1p that catalyzes the first step of diphthamide biosynthesis
S000001675	YKL192C	ACP1	Acyl Carrier Protein	80160	80537	-1	Mitochondrial matrix acyl carrier protein	Mitochondrial matrix acyl carrier protein; involved in biosynthesis of octanoate, which is a precursor to lipoic acid; coordinates mitochondrial fatty acid and iron sulfur cluster metabolism; activated by phosphopantetheinylation catalyzed by Ppt2p
S000001676	YKL193C	SDS22	homolog of S. pombe SDS22	78866	79882	-1	Regulatory subunit of the type 1 protein phosphatase (PP1) Glc7p	Regulatory subunit of the type 1 protein phosphatase (PP1) Glc7p; whether it functions as a positive or negative regulator of Glc7p is controversial; involved in the regulation of Glc7p nuclear localization and function
S000001677	YKL194C	MST1	Mitochondrial aminoacyl-tRNA Synthetase, Threonine	77258	78646	-1	Mitochondrial threonyl-tRNA synthetase	Mitochondrial threonyl-tRNA synthetase; aminoacylates both the canonical threonine tRNA tT(UGU)Q1 and the unusual threonine tRNA tT(UAG)Q2 in vitro; lacks a typical editing domain, but has pre-transfer editing activity stimulated by the unusual tRNA-Thr
S000001678	YKL195W	MIA40	Mitochondrial intermembrane space Import and Assembly	75821	77032	1	Import and assembly protein in mitochondrial intermembrane space	Import and assembly protein in mitochondrial intermembrane space; component of MIA pathway which mediates import and oxidative folding of substrates including small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p via a chaperone-like activity; forms a dimer that binds iron-sulfur cluster in vitro
S000001680	YKL197C	PEX1	PEroXin	70734	73865	-1	AAA-peroxin	AAA-peroxin; heterodimerizes with AAA-peroxin Pex6p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol; induced by oleic acid and upregulated during anaerobiosis; mutations in human PEX1 can lead to severe peroxisomal disorders and early death
S000001681	YKL198C	PTK1	Putative serine/Threonine protein Kinase	68232	70220	-1	Putative serine/threonine protein kinase	Putative serine/threonine protein kinase; regulates spermine uptake; involved in polyamine transport; possible mitochondrial protein; PTK1 has a paralog, PTK2, that arose from the whole genome duplication
S000001682	YKL199C	""	""	68270	69109	-1	Merged open reading frame	Merged open reading frame; does not encode a discrete protein; YKL199C was originally annotated as an independent ORF, but as a result of a sequence change, it was merged with an adjacent ORF into a single reading frame, designated YKL198C
S000001683	YKL200C	""	""	65913	67109	-1	Merged open reading frame	Merged open reading frame; does not encode a discrete protein; YKL200C was originally annotated as an independent ORF, but as a result of a sequence change, it was merged with an adjacent ORF into a single reading frame, designated YKL201C
S000001684	YKL201C	MNN4	MaNNosyltransferase	63930	67466	-1	Putative positive regulator of mannosylphosphate transferase Mnn6p	Putative positive regulator of mannosylphosphate transferase Mnn6p; involved in mannosylphosphorylation of N-linked oligosaccharides; may also function as a mannosylphosphate transferase; expression increases in late-logarithmic and stationary growth phases; coding sequence contains length polymorphisms in different strains; MNN4 has a paralog, YJR061W, that arose from the whole genome duplication
S000001685	YKL202W	""	""	63821	64402	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000001686	YKL203C	TOR2	Target Of Rapamycin	55935	63359	-1	PIK-related protein kinase and rapamycin target	PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates plasma membrane (PM) homeostasis and cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; PM retention is dependent on Avo3p; malonyl-CoA is a direct ATP-competitive inhibitor of TORC1; TOR2 has a paralog, TOR1, that arose from the whole genome duplication
S000001687	YKL204W	EAP1	EIF4E-Associated Protein	53704	55602	1	eIF4E-associated protein, competes with eIF4G for binding to eIF4E	eIF4E-associated protein, competes with eIF4G for binding to eIF4E; accelerates mRNA degradation by promoting decapping, facilitated by interaction with eIF4E; essential for Puf5p mediated repression; associates with Puf5p and Dhh1p; inhibits cap-dependent translation; functions independently of eIF4E to maintain genetic stability; plays a role in cell growth, implicated in the TOR signaling cascade
S000001688	YKL205W	LOS1	Loss Of Suppression	50051	53353	1	Nuclear pore protein	Nuclear pore protein; involved in nuclear export of pre-tRNA and in re-export of mature tRNAs after their retrograde import from the cytoplasm; deletion mutation extends replicative lifespan, as does exclusion of Los1p from the nucleus in response to caloric restriction
S000001689	YKL206C	ADD66	Alpha1-proteinase inhibitor-Degradation Deficient	49006	49809	-1	Protein involved in 20S proteasome assembly	Protein involved in 20S proteasome assembly; forms a heterodimer with Pba1p that binds to proteasome precursors; interaction with Pba1p-Add66p may affect function of the mature proteasome and its role in maintaining respiratory metabolism; similar to human PAC2 constituent of the PAC1-PAC2 complex involved in proteasome assembly
S000001690	YKL207W	EMC3	ER Membrane protein Complex	48194	48955	1	Member of conserved ER transmembrane complex	Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; required for respiratory growth; null mutant displays induction of the unfolded protein response; homologous to worm Y62E10A.10/EMC-3, fly CG6750, human TMEM111
S000001691	YKL208W	CBT1	Cytochrome B Termination	47157	47972	1	Protein involved in 5' RNA end processing	Protein involved in 5' RNA end processing; substrates include mitochondrial COB, 15S_rRNA, and RPM1 transcripts; may also have a role in 3' end processing of the COB pre-mRNA; displays genetic interaction with cell cycle-regulated kinase Dbf2p
S000001693	YKL210W	UBA1	UBiquitin Activating	39163	42237	1	Ubiquitin activating enzyme (E1)	Ubiquitin activating enzyme (E1); involved in ubiquitin-mediated protein degradation and essential for viability; protein abundance increases in response to DNA replication stress
S000001694	YKL211C	TRP3	TRyPtophan	36699	38153	-1	Indole-3-glycerol-phosphate synthase	Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
S000001695	YKL212W	SAC1	Suppressor of ACtin	34543	36414	1	Phosphatidylinositol phosphate phosphatase	Phosphatidylinositol phosphate phosphatase; role in early and medial Golgi PtdIns4P hydrolysis; regulated by Vps74p interaction; ER localized transmembrane protein which cycles through the Golgi; role in protein trafficking, processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis by modulating PtdIns(4)P metabolism; PtdIns4P PPase activity is crucial for autophagosome-vacuole fusion during autophagy; human homolog SACM1L complements autophagy defects of the null
S000001696	YKL213C	DOA1	Degradation Of Alpha	31960	34107	-1	WD-repeat protein involved in Ub-mediated proteolysis	WD-repeat protein involved in Ub-mediated proteolysis; K48-, K29-specific Ub chain binding protein involved in Ub homeostasis; substrate-recruiting adaptor for Cdc48p in mitochondria-associated degradation; inhibits degradation of Ufd2p-dependent substrates; facilitates proteolysis of Cse4p, a centromeric H3-like protein; required for ribophagy; promotes NHEJ in postdiauxic/stationary phase; protein increases in abundance and relocalizes from nucleus to nuclear periphery upon replication stress
S000001697	YKL214C	YRA2	Yeast RNA Annealing protein	31082	31693	-1	Member of the REF (RNA and export factor binding proteins) family	Member of the REF (RNA and export factor binding proteins) family; when overexpressed, can substitute for the function of Yra1p in export of poly(A)+ mRNA from the nucleus
S000001698	YKL215C	OXP1	OXoProlinase	26827	30687	-1	5-oxoprolinase	5-oxoprolinase; enzyme is ATP-dependent and functions as a dimer; similar to mouse Oplah gene; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress
S000001699	YKL216W	URA1	URAcil requiring	25215	26159	1	Dihydroorotate dehydrogenase	Dihydroorotate dehydrogenase; catalyzes the fourth enzymatic step in the de novo biosynthesis of pyrimidines, converting dihydroorotic acid into orotic acid
S000001700	YKL217W	JEN1	""	22234	24084	1	Monocarboxylate/proton symporter of the plasma membrane	Monocarboxylate/proton symporter of the plasma membrane; transport activity is dependent on the pH gradient across the membrane; mediates high-affinity uptake of carbon sources lactate, pyuvate, and acetate, and also of the micronutrient selenite, whose structure mimics that of monocarboxylates; expression and localization are tightly regulated, with transcription repression, mRNA degradation, and protein endocytosis and degradation all occurring in the presence of glucose
S000001701	YKL218C	SRY1	Serine Racemase of Yeast	17359	18339	-1	3-hydroxyaspartate dehydratase	3-hydroxyaspartate dehydratase; deaminates L-threo-3-hydroxyaspartate to form oxaloacetate and ammonia; required in the presence of hydroxyaspartate; highly similar to mouse serine racemase (Srr) but has no serine racemase activity
S000001703	YKL220C	FRE2	Ferric REductase	9091	11226	-1	Ferric reductase and cupric reductase	Ferric reductase and cupric reductase; reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low iron levels but not by low copper levels
S000001704	YKL221W	MCH2	MonoCarboxylate permease Homologue	6107	7528	1	Protein with similarity to mammalian monocarboxylate permeases	Protein with similarity to mammalian monocarboxylate permeases; monocarboxylate permeases are involved in transport of monocarboxylic acids across the plasma membrane but mutant is not deficient in monocarboxylate transport
S000001705	YKL222C	""	""	3503	5620	-1	Protein of unknown function	Protein of unknown function; may interact with ribosomes, based on co-purification experiments; similar to transcriptional regulators from the zinc cluster (binuclear cluster) family; null mutant is sensitive to caffeine
S000001709	YKR001C	VPS1	Vacuolar Protein Sorting	440608	442722	-1	Dynamin-like GTPase required for vacuolar sorting	Dynamin-like GTPase required for vacuolar sorting; promotes fission of retrograde transport carriers from endosome; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis
S000001710	YKR002W	PAP1	Poly(A) Polymerase	443232	444938	1	Poly(A) polymerase	Poly(A) polymerase; one of three factors required for mRNA 3'-end polyadenylation, forms multiprotein complex with polyadenylation factor I (PF I), also required for mRNA nuclear export; may also polyadenylate rRNAs; required for gene looping
S000001711	YKR003W	OSH6	OxySterol binding protein Homolog	445381	446727	1	Oxysterol-binding  phosphatidylserine transferase	Oxysterol-binding  phosphatidylserine transferase; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability; GFP-fusion protein localizes to the cell periphery; overexpression extends lifespan by promoting vacuolar fusion; OSH6 has a paralog, OSH7, that arose from the whole genome duplication
S000001712	YKR004C	ECM9	ExtraCellular Mutant	446799	448036	-1	Non-essential protein of unknown function	Non-essential protein of unknown function
S000001713	YKR005C	""	""	448521	450167	-1	Putative protein of unknown function	Putative protein of unknown function
S000001714	YKR006C	MRPL13	Mitochondrial Ribosomal Protein, Large subunit	450423	451217	-1	Mitochondrial ribosomal protein of the large subunit	Mitochondrial ribosomal protein of the large subunit; not essential for mitochondrial translation
S000001715	YKR007W	MEH1	Multicopy suppressor of Ers1 Hygromycin B sensitivity	451434	451988	1	Subunit of EGO/GSE complex	Subunit of EGO/GSE complex; vacuolar/endosomal membrane-associated EGO/GSE complex regulates exit from rapamycin-induced growth arrest, stimulating microautophagy and sorting of Gap1p from endosome to plasma membrane; targeted to vacuole via AP-3 pathway; loss results in defect in vacuolar acidification
S000001716	YKR008W	RSC4	Remodel the Structure of Chromatin	452201	454078	1	Component of the RSC chromatin remodeling complex	Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p
S000001717	YKR009C	FOX2	Fatty acid OXidation	454352	457054	-1	3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase	3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase; multifunctional enzyme of the peroxisomal fatty acid beta-oxidation pathway; mutation is functionally complemented by human HSD17B4
S000001718	YKR010C	TOF2	TOpoisomerase I-interacting Factor	458924	461239	-1	Protein required for rDNA silencing and mitotic rDNA condensation	Protein required for rDNA silencing and mitotic rDNA condensation; stimulates Cdc14p phosphatase activity and biphasic release to promote rDNA repeat segregation; required for condensin recruitment to the replication fork barrier site; TOF2 has a paralog, NET1, that arose from the whole genome duplication
S000001719	YKR011C	""	""	461635	462696	-1	Protein of unknown function	Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; protein abundance increases in response to DNA replication stress
S000001720	YKR012C	""	""	463739	464116	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PRY2
S000001721	YKR013W	PRY2	Pathogen Related in Yeast	463959	464948	1	Sterol binding protein involved in the export of acetylated sterols	Sterol binding protein involved in the export of acetylated sterols; role in sterol export is redundant with that of PRY1; redundant role with PRY1 and PRY3 in the export of free fatty acids; may be involved in detoxification of hydrophobic compounds; secreted glycoprotein and member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins)
S000001722	YKR014C	YPT52	Yeast Protein Two	465367	466071	-1	Endosomal Rab family GTPase	Endosomal Rab family GTPase; required for vacuolar protein sorting, endocytosis and multivesicular body (MVB) biogenesis and sorting; required for localization of the CORVET complex to endosomes; involved in autophagy and ionic stress tolerance; similar to Vps21p and Ypt53p; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress
S000001723	YKR015C	""	""	466961	468667	-1	Putative protein of unknown function	Putative protein of unknown function
S000001724	YKR016W	MIC60	MItochondrial contact site and Cristae organizing system	469717	471339	1	Component of the MICOS complex	Component of the MICOS complex; MICOS (formerly MINOS or MitOS) is a mitochondrial inner membrane complex that extends into the intermembrane space and has a role in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane; Mic60p is also involved in import of intermembrane space (IMS) proteins, probably by positioning Mia40p relative to the TOM complex to receive incoming proteins; ortholog of mammalian mitofilin
S000001725	YKR017C	HEL1	Histone E3 Ligase	471694	473349	-1	RING finger ubiquitin ligase (E3)	RING finger ubiquitin ligase (E3); involved in ubiquitination and degradation of excess histones; interacts with Ubc4p and Rad53p; null mutant sensitive to hydroxyurea (HU)
S000001726	YKR018C	""	""	473723	475900	-1	Protein of unknown function	Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress; YKR018C has a paralog, IML2, that arose from the whole genome duplication
S000001727	YKR019C	IRS4	Increased rDNA Silencing	476216	478063	-1	EH domain-containing protein	EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
S000001728	YKR020W	VPS51	Vacuolar Protein Sorting	478338	478832	1	Component of the GARP (Golgi-associated retrograde protein) complex	Component of the GARP (Golgi-associated retrograde protein) complex; GARP is required for the recycling of proteins from endosomes to the late Golgi, and for mitosis after DNA damage induced checkpoint arrest; links the (VFT/GARP) complex to the SNARE Tlg1p; members of the GARP complex are Vps51p-Vps52p-Vps53p-Vps54p
S000001729	YKR021W	ALY1	Arrestin-Like Yeast protein	479234	481981	1	Alpha arrestin, substrate of calcineurin	Alpha arrestin, substrate of calcineurin; controls nutrient-mediated intracellular sorting of permease Gap1p; interacts with AP-1 subunit Apl4p; dephosphorylation of Aly1p required for the endocytosis of Dip5p; may regulate endocytosis of plasma membrane proteins by recruiting ubiquitin ligase Rsp5p to plasma membrane targets; ALY1 has a paralog, ALY2, that arose from the whole genome duplication
S000001730	YKR022C	NTR2	NineTeen complex Related protein	482171	483139	-1	Essential protein that forms a dimer with Ntr1p	Essential protein that forms a dimer with Ntr1p; also forms a trimer, with Ntr2p and the DExD/H-box RNA helicase Prp43p, that is involved in spliceosome disassembly
S000001731	YKR023W	RQT4	Ribosome-Quality control Trigger factor	483419	485011	1	Subunit of ribosome-associated quality control trigger complex (RQT)	Subunit of ribosome-associated quality control trigger complex (RQT); the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
S000001732	YKR024C	DBP7	Dead Box Protein	485144	487372	-1	Putative ATP-dependent RNA helicase of the DEAD-box family	Putative ATP-dependent RNA helicase of the DEAD-box family; involved in ribosomal biogenesis; required at post-transcriptional step for efficient retrotransposition; essential for growth under anaerobic conditions
S000001733	YKR025W	RPC37	RNA Polymerase C	487770	488618	1	RNA polymerase III subunit C37	RNA polymerase III subunit C37
S000001734	YKR026C	GCN3	General Control Nonderepressible	488738	489655	-1	Alpha subunit of translation initiation factor eIF2B (eIF2Bα)	Alpha subunit of translation initiation factor eIF2B (eIF2Bα); guanine-nucleotide exchange factor for eIF2; mutations in eIF2Bα alter localization of eIF2B; activity regulated by phosphorylated eIF2; positive regulator of GCN4 expression; mutations in human ortholog EIF2B2 cause fatal brain disease childhood ataxia with central nervous system hypomyelination (CACH); human ortholog EIF2B1 can complement yeast null mutant
S000001735	YKR027W	BCH2	Bud7 and Chs6 Homolog	491364	493661	1	Member of the ChAPs (Chs5p-Arf1p-binding proteins) family	Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BCH2 has a paralog, CHS6, that arose from the whole genome duplication
S000001736	YKR028W	SAP190	Sit4 Associated Protein	494257	497358	1	Protein that forms a complex with the Sit4p protein phosphatase	Protein that forms a complex with the Sit4p protein phosphatase; required for Sit4p function; member of a family of similar proteins including Sap4p, Sap155p, and Sap185p; SAP190 has a paralog, SAP185, that arose from the whole genome duplication
S000001737	YKR029C	SET3	SET domain-containing	497578	499833	-1	Defining member of the SET3 histone deacetylase complex	Defining member of the SET3 histone deacetylase complex; which is a meiosis-specific repressor of sporulation genes; necessary for efficient transcription by RNAPII; one of two yeast proteins that contains both SET and PHD domains; SET3 has a paralog, SET4, that arose from the whole genome duplication
S000001738	YKR030W	GMH1	Gea1-6 Membrane-associated High-copy suppressor	500282	501103	1	Golgi membrane protein of unknown function	Golgi membrane protein of unknown function; interacts with Gea1p and Gea2p; required for localization of Gea2p; computational analysis suggests a possible role in either cell wall synthesis or protein-vacuolar targeting; conserved from yeast to human
S000001739	YKR031C	SPO14	SPOrulation	501344	506395	-1	Phospholipase D	Phospholipase D; catalyzes the hydrolysis of phosphatidylcholine, producing choline and phosphatidic acid; involved in Sec14p-independent secretion; required for meiosis and spore formation; differently regulated in secretion and meiosis; participates in transcription initiation and/or early elongation of specific genes; interacts with 'foot domain' of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions
S000001740	YKR032W	""	""	506517	506831	1	Putative protein of unknown function	Putative protein of unknown function; conserved among S. cerevisiae strains
S000001741	YKR033C	""	""	506879	507304	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene DAL80
S000001742	YKR034W	DAL80	Degradation of Allantoin	506898	507707	1	Negative regulator of genes in multiple nitrogen degradation pathways	Negative regulator of genes in multiple nitrogen degradation pathways; expression is regulated by nitrogen levels and by Gln3p; member of the GATA-binding family, forms homodimers and heterodimers with Gzf3p; DAL80 has a paralog, GZF3, that arose from the whole genome duplication
S000001743	YKR035C	OPI8	OverProducer of Inositol	507920	508561	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified gene DID2/YKR035W-A
S000001744	YKR036C	CAF4	CCR4 Associated Factor	508702	510633	-1	WD40 repeat-containing protein associated with the CCR4-NOT complex	WD40 repeat-containing protein associated with the CCR4-NOT complex; interacts in a Ccr4p-dependent manner with Ssn2p; also interacts with Fis1p, Mdv1p and Dnm1p and plays a role in mitochondrial fission; CAF4 has a paralog, MDV1, that arose from the whole genome duplication
S000001745	YKR037C	SPC34	Spindle Pole Component	510910	511797	-1	Essential subunit of the Dam1 complex (aka DASH complex)	Essential subunit of the Dam1 complex (aka DASH complex); complex couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body
S000001746	YKR038C	KAE1	Kinase-Associated Endopeptidase	511999	513159	-1	ADP/GDP nucleotidase, essential functional component of KEOPS complex,	ADP/GDP nucleotidase, essential functional component of KEOPS complex, with Bud32p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and telomeric TG1-3 recombination; may have role in transcription; homolog of human OSGEP
S000001747	YKR039W	GAP1	General Amino acid Permease	515063	516871	1	General amino acid permease	General amino acid permease; involved in uptake of all L-amino acids, some D-amino acids, related compounds, toxic analogs and polyamines; regulated by nitrogen source; targeted to vacuole via Vps10p-dependent endosomal vacuolar protein sorting pathway; essential for invasive growth
S000001748	YKR040C	""	""	518063	518566	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YKR041W
S000001749	YKR041W	""	""	518198	518950	1	Protein of unknown function	Protein of unknown function; localizes to the mitotic spindle; overexpression of YKR041W affects endocytic protein trafficking
S000001750	YKR042W	UTH1	yoUTH	519527	520624	1	Mitochondrial inner membrane protein	Mitochondrial inner membrane protein; role in mitophagy is disputed; implicated in cell wall biogenesis, the oxidative stress response, life span during starvation, and cell death; SUN family member; UTH1 has a paralog, NCA3, that arose from the whole genome duplication
S000001751	YKR043C	SHB17	SedoHeptulose 1,7-Bisphosphatase	520897	521712	-1	Sedoheptulose bisphosphatase involved in riboneogenesis	Sedoheptulose bisphosphatase involved in riboneogenesis; dephosphorylates sedoheptulose 1,7-bisphosphate, which is converted via the nonoxidative pentose phosphate pathway to ribose-5-phosphate; facilitates the conversion of glycolytic intermediates to pentose phosphate units; also has fructose 1,6-bisphosphatase activity but this is probably not biologically relevant, since deletion does not affect FBP levels; GFP-fusion protein localizes to the cytoplasm and nucleus
S000001752	YKR044W	UIP5	Ulp1 Interacting Protein	522015	523346	1	Protein of unknown function that interacts with Ulp1p	Protein of unknown function that interacts with Ulp1p; a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates
S000001753	YKR045C	""	""	523418	523969	-1	Putative protein of unknown function	Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm
S000001754	YKR046C	PLN1	PeriLipiN	524223	525074	-1	Perilipin, involved in formation and stability of lipid droplets	Perilipin, involved in formation and stability of lipid droplets; expression pattern suggests a role in respiratory growth; computational analysis of large-scale protein-protein interaction data suggests a role in ATP/ADP exchange
S000001755	YKR047W	""	""	525257	525562	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NAP1
S000001756	YKR048C	NAP1	Nucleosome Assembly Protein	525387	526640	-1	Histone chaperone and assembly chaperone for Rps6a/b	Histone chaperone and assembly chaperone for Rps6a/b; involved in histone exchange, replacing histone H2A-H2B dimers or histone variant dimers from assembled nucleosomes; promotes solubility of Rps6a, and 40S ribosomal subunit biogenesis; role in transport of H2A and H2B histones to the nucleus; regulates microtubule dynamics during mitosis; interacts with mitotic cyclin Clb2p; controls bud morphogenesis; phosphorylated by CK2; protein abundance increases in response to DNA replication stress
S000001757	YKR049C	FMP46	Found in Mitochondrial Proteome	526830	527231	-1	Putative redox protein containing a thioredoxin fold	Putative redox protein containing a thioredoxin fold; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
S000001758	YKR050W	TRK2	TRansport of potassium (K)	527815	530484	1	Component of the Trk1p-Trk2p potassium transport system	Component of the Trk1p-Trk2p potassium transport system; contributes to K(+) supply and maintenance of plasma-membrane potential; TRK2 has a paralog, TRK1, that arose from the whole genome duplication
S000001759	YKR051W	HFL1	Has Fused Lysosomes	530946	532202	1	Vacuole membrane protein that interacts with Atg8p	Vacuole membrane protein that interacts with Atg8p; functions in the vacuolar membrane recruitment of Atg8p to facilitate the degradation of vacuolar integral membrane proteins during early-stationary vacuole turnover (EVT), a general degradation process for vacuolar integral membrane proteins when cells enter stationary phase; null mutant is defective in stationary phase-induced vacuolar microdomain formation; homolog of S. pombe hfl1; homolog of human TMEM184
S000001760	YKR052C	MRS4	Mitochondrial RNA Splicing	532550	533464	-1	Iron transporter of the mitochondrial carrier family	Iron transporter of the mitochondrial carrier family; mediates Fe2+ transport across the inner mitochondrial membrane; active under low-iron conditions; may transport other cations; protein abundance increases in response to DNA replication stress; MRS4 has a paralog, MRS3, that arose from the whole genome duplication
S000001761	YKR053C	YSR3	Yeast Sphingolipid Resistance	534067	535281	-1	Dihydrosphingosine 1-phosphate phosphatase	Dihydrosphingosine 1-phosphate phosphatase; membrane protein involved in sphingolipid metabolism; YSR3 has a paralog, LCB3, that arose from the whole genome duplication
S000001762	YKR054C	DYN1	DYNein	535647	547925	-1	Cytoplasmic heavy chain dynein	Cytoplasmic heavy chain dynein; microtubule motor protein; member of the AAA+ protein family, required for anaphase spindle elongation; involved in spindle assembly, chromosome movement, and spindle orientation during cell division, targeted to microtubule tips by Pac1p; motility along microtubules inhibited by She1p
S000001763	YKR055W	RHO4	Ras HOmolog	548216	549091	1	Non-essential small GTPase	Non-essential small GTPase; member of the Rho/Rac subfamily of Ras-like proteins; likely to be involved in the establishment of cell polarity; has long N-terminal extension that plays an important role in Rho4p function and is shared with Rho4 homologs in other yeasts and filamentous fungi
S000001764	YKR056W	TRM2	tRNA Methyltransferase	549448	551367	1	tRNA methyltransferase	tRNA methyltransferase; 5-methylates the uridine residue at position 54 of tRNAs and may also have a role in tRNA stabilization or maturation; involved in the methylation of guanosine and uridine ribonucleosides in mRNA; endo-exonuclease with a role in DNA repair
S000001765	YKR057W	RPS21A	Ribosomal Protein of the Small subunit	551657	552242	1	Protein component of the small (40S) ribosomal subunit	Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21A has a paralog, RPS21B, that arose from the whole genome duplication
S000001766	YKR058W	GLG1	Glycogenin-Like Gene	552770	554620	1	Glycogenin glucosyltransferase	Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; SWAT-GFP and mCherry fusion proteins localize to the cell periphery and vacuole respectively; similar to mammalian glycogenin; GLG1 has a paralog, GLG2, that arose from the whole genome duplication
S000001767	YKR059W	TIF1	Translation Initiation Factor	554987	556174	1	Translation initiation factor eIF4A	Translation initiation factor eIF4A; DEAD-box RNA helicase that couples ATPase activity to mRNA binding and unwinding; ATP hydrolysis accelerates the recruitment of mRNA during 48S preinitiation complex (PIC) assembly; ATPase activity stimulated by either eIF4G1 or by the PIC in an eIF3g and eIFGi subunit-dependent manner, independent of eIF4E and eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) with eIF4E and eIF4G; protein abundance increases in response to DNA replication stress
S000001768	YKR060W	UTP30	U Three Protein	556518	557342	1	Subunit of U3-containing 90S preribosome complex	Subunit of U3-containing 90S preribosome complex; complex is involved in production of 18S rRNA and assembly of small ribosomal subunit; Utp30p is peripherally associated with 90S preribosome and appears partially redundant with its paralog Cic1p
S000001769	YKR061W	KTR2	Kre Two Related	557677	558954	1	Mannosyltransferase involved in N-linked protein glycosylation	Mannosyltransferase involved in N-linked protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR2 has a paralog, YUR1, that arose from the whole genome duplication
S000001770	YKR062W	TFA2	Transcription Factor A	559666	560652	1	TFIIE small subunit	TFIIE small subunit; involved in RNA polymerase II transcription initiation
S000001771	YKR063C	LAS1	Lethal in the Absence of SSD1-v	560789	562297	-1	Endonuclease involved in pre-rRNA processing at both ends of ITS2	Endonuclease involved in pre-rRNA processing at both ends of ITS2; functions with Grc3p in a conserved mechanism to cleave pre-rRNA at a specific site during rRNA processing and ribosome biogenesis; may coordinate the action of the Rat1p-Rai1p exoRNAse; required for the G1/S cell cycle transition; human ortholog is Las1L; mutants require the SSD1-v allele for viability
S000001772	YKR064W	OAF3	Oleate Activated transcription Factor	562547	565138	1	Putative transcriptional repressor with Zn(2)-Cys(6) finger	Putative transcriptional repressor with Zn(2)-Cys(6) finger; negatively regulates transcription in response to oleate levels, based on mutant phenotype and localization to oleate-responsive promoters; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress
S000001773	YKR065C	PAM17	Presequence translocase-Associated Motor	565299	565892	-1	Constituent of the TIM23 complex	Constituent of the TIM23 complex; proposed alternatively to be a component of the import motor (PAM complex) or to interact with and modulate the core TIM23 (Translocase of the Inner mitochondrial Membrane) complex; protein abundance increases in response to DNA replication stress
S000001774	YKR066C	CCP1	Cytochrome c Peroxidase	566113	567198	-1	Mitochondrial cytochrome-c peroxidase	Mitochondrial cytochrome-c peroxidase; degrades reactive oxygen species in mitochondria; acts as hydrogen peroxide sensor in mitochondria, involved in the response to oxidative stress
S000001775	YKR067W	GPT2	Glycerol-3-Phosphate acylTransferase	567918	570149	1	Glycerol-3-phosphate/dihydroxyacetone phosphate sn-1 acyltransferase	Glycerol-3-phosphate/dihydroxyacetone phosphate sn-1 acyltransferase; located in lipid particles and the ER; involved in the stepwise acylation of glycerol-3-phosphate and dihydroxyacetone in lipid biosynthesis; the most conserved motifs and functionally relevant residues are oriented towards the ER lumen
S000001776	YKR068C	BET3	Blocked Early in Transport	570328	570909	-1	Core component of transport protein particle (TRAPP) complexes I-III	Core component of transport protein particle (TRAPP) complexes I-III; TRAPP complexes are related multimeric guanine nucleotide-exchange factors for the GTPase Ypt1, regulating ER-Golgi traffic (TRAPPI), intra-Golgi traffic (TRAPPII), endosome-Golgi traffic (TRAPPII and III) and autophagy (TRAPPIII); hydrophilic homodimeric protein that acts in conjunction with SNARE proteins in targeting and fusion of ER to Golgi transport vesicles
S000001777	YKR069W	MET1	METhionine requiring	571612	573393	1	S-adenosyl-L-methionine uroporphyrinogen III transmethylase	S-adenosyl-L-methionine uroporphyrinogen III transmethylase; involved in the biosynthesis of siroheme, a prosthetic group used by sulfite reductase; required for sulfate assimilation and methionine biosynthesis
S000001778	YKR070W	""	""	573574	574632	1	Putative protein of unknown function	Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
S000001779	YKR071C	DRE2	Derepressed for Ribosomal protein S14 Expression	574934	575980	-1	Component of the cytosolic Fe-S protein assembly (CIA) machinery	Component of the cytosolic Fe-S protein assembly (CIA) machinery; contains an Fe-S cluster that receives electrons from NADPH via the action of Tah18p in an early step in the CIA pathway; ortholog of human Ciapin1; protein abundance increases in response to DNA replication stress; inviability of the null mutant is functionally complemented by human CIAPIN1
S000001780	YKR072C	SIS2	SIt4 Suppressor	576435	578123	-1	Negative regulatory subunit of protein phosphatase 1 (Ppz1p)	Negative regulatory subunit of protein phosphatase 1 (Ppz1p); involved in coenzyme A biosynthesis; subunit of phosphopantothenoylcysteine decarboxylase (PPCDC: Cab3p, Sis2p, Vhs3p) complex and the CoA-Synthesizing Protein Complex (CoA-SPC: Cab2p, Cab3p, Cab4p, Cab5p, Sis2p and Vhs3p); SIS2 has a paralog, VHS3, that arose from the whole genome duplication
S000001781	YKR073C	""	""	578187	578507	-1	Putative protein of unknown function	Putative protein of unknown function; conserved across S. cerevisiae strains
S000001782	YKR074W	AIM29	Altered Inheritance rate of Mitochondria	579209	579676	1	Zpr1p co-chaperone	Zpr1p co-chaperone; recognizes eEF1A in GTP-bound, pre-hydrolysis conformation, dampens folding complex GTPase activity to drive client release from Zpr1p
S000001783	YKR075C	""	""	579827	580750	-1	Protein of unknown function	Protein of unknown function; similar to Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; YKR075C has a paralog, YOR062C, that arose from the whole genome duplication
S000001784	YKR076W	ECM4	ExtraCellular Mutant	582283	583395	1	S-glutathionyl-(chloro)hydroquinone reductase (GS-HQR)	S-glutathionyl-(chloro)hydroquinone reductase (GS-HQR); glutathione transferase involved in cell-surface biosynthesis and architecture; catalyzes glutathione (GSH)-dependent reduction of GS-trichloro-p-hydroquinone to trichloro-p-hydroquinone; expression upregulated upon exposure to genotoxic agents, such as methyl methanesulfonate, cisplatin and bleomycin; not an essential gene; similar to YGR154C; green fluorescent protein (GFP)-fusion protein localizes to cytoplasm
S000001785	YKR077W	MSA2	Mbf and Sbf Associated	583653	584744	1	Putative transcriptional activator	Putative transcriptional activator; interacts with G1-specific transcription factor MBF and G1-specific promoters; MSA2 has a paralog, MSA1, that arose from the whole genome duplication
S000001786	YKR078W	VPS501	VPS5-related	584952	586709	1	Vacuolar SNX-BAR protein, regulates TORC1 signaling during autophagy i	Vacuolar SNX-BAR protein, regulates TORC1 signaling during autophagy induction; binds phosphatidylinositol-3-phosphate; vacuolar localization of Vps501p is dependent upon direct interaction with Iml1p and a unique lipid binding specificity that is also required for its function; VPS501 has paralog VPS5 that arose from the whole genome duplication
S000001787	YKR079C	TRZ1	tRNase Z	586789	589305	-1	tRNA 3'-end processing endonuclease tRNase Z	tRNA 3'-end processing endonuclease tRNase Z; also localized to mitochondria and interacts genetically with Rex2 exonuclease; homolog of the human candidate prostate cancer susceptibility gene ELAC2
S000001788	YKR080W	MTD1	Methylene Tetrahydrafolate Dehydrogenase	590395	591357	1	NAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase	NAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase; plays a catalytic role in oxidation of cytoplasmic one-carbon units; expression is regulated by Bas1p and Bas2p, repressed by adenine, and may be induced by inositol and choline
S000001789	YKR081C	RPF2	Ribosome Production Factor	591506	592540	-1	Essential protein involved in rRNA maturation and ribosomal assembly	Essential protein involved in rRNA maturation and ribosomal assembly; involved in the processing of pre-rRNA and the assembly of the 60S ribosomal subunit; interacts with ribosomal protein L11; localizes predominantly to the nucleolus; constituent of 66S pre-ribosomal particles
S000001790	YKR082W	NUP133	NUclear Pore	592825	596298	1	Subunit of Nup84p subcomplex of nuclear pore complex (NPC)	Subunit of Nup84p subcomplex of nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis; is involved in establishment of a normal nucleocytoplasmic concentration gradient of GTPase Gsp1p; also plays roles in several processes that may require localization of genes or chromosomes at nuclear periphery, including double-strand break repair, transcription and chromatin silencing; relocalizes to cytosol in response to hypoxia; homolog of human NUP133
S000001791	YKR083C	DAD2	Duo1 And Dam1 interacting	596421	596822	-1	Essential subunit of the Dam1 complex (aka DASH complex)	Essential subunit of the Dam1 complex (aka DASH complex); complex couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
S000001792	YKR084C	HBS1	Hsp70 subfamily B Suppressor	597055	598890	-1	GTPase with similarity to translation release factors	GTPase with similarity to translation release factors; together with binding partner Dom34p, facilitates ribosomal subunit dissociation and peptidyl-tRNA release when translation is stalled, particularly in 3' UTRs; genetically implicated in mRNA no-go decay; HBS1 has a paralog, SKI7, that arose from the whole genome duplication
S000001793	YKR085C	MRPL20	Mitochondrial Ribosomal Protein, Large subunit	599094	599681	-1	Mitochondrial ribosomal protein of the large subunit	Mitochondrial ribosomal protein of the large subunit
S000001795	YKR087C	OMA1	Overlapping activity with M-AAA protease	603195	604232	-1	Metalloendopeptidase of the mitochondrial inner membrane	Metalloendopeptidase of the mitochondrial inner membrane; important for adaptive responses to various homeostatic insults, preservation of normal mitochondrial function under damage-eliciting conditions, and stability of respiratory supercomplexes; involved in turnover of membrane-embedded proteins; mediates degradation of Cox1p in coa2 mutant cells; zebrafish ortholog has a role in organ development and mouse ortholog is also required for respiratory supercomplex stability
S000001796	YKR088C	TVP38	Tlg2-Vesicle Protein	604405	605418	-1	Integral membrane protein	Integral membrane protein; localized to late Golgi vesicles along with the v-SNARE Tlg2p; required for asymmetric localization of Kar9p during mitosis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern
S000001797	YKR089C	TGL4	TriacylGlycerol Lipase	605633	608365	-1	Multifunctional lipase/hydrolase/phospholipase	Multifunctional lipase/hydrolase/phospholipase; triacylglycerol lipase, steryl ester hydrolase, and Ca2+-independent phospholipase A2; catalyzes acyl-CoA dependent acylation of LPA to PA; required with Tgl3p for timely bud formation; phosphorylated and activated by Cdc28p; TGL4 has a paralog, TGL5, that arose from the whole genome duplication
S000001798	YKR090W	PXL1	PaXillin-Like protein	608946	611066	1	Protein that localizes to sites of polarized growth	Protein that localizes to sites of polarized growth; required for selection and/or maintenance of polarized growth sites, may modulate signaling by the GTPases Cdc42p and Rho1p; contains LIM domains and has similarity to metazoan paxillin; relocalizes from bud neck to cytoplasm upon DNA replication stress
S000001799	YKR091W	SRL3	Suppressor of rad53 Lethality	611525	612265	1	Repressor of transcription at Start	Repressor of transcription at Start; binds to SBF (Swi4p-Swi6p) at SCB promoter elements in G1 to repress transcription; inhibitor of Start that retains Cdc28-Cln3 at the ER; acts with Ydj1p to promote release of the complex in a growth-mediated positive feedback loop to trigger Start; Srl3p is unstable and degraded by SCF-Grr1 after Cdc28p-dependent phosphorylation; contains a GTB (G1/S transcription factor binding) motif; binds SBF-regulated promoters in HU-treated cells; Cdc28p substrate
S000001800	YKR092C	SRP40	Serine Rich Protein	612665	613885	-1	Nucleolar serine-rich protein	Nucleolar serine-rich protein; role in preribosome assembly or transport; may function as a chaperone of small nucleolar ribonucleoprotein particles (snoRNPs); immunologically and structurally to rat Nopp140
S000001801	YKR093W	PTR2	Peptide TRansport	615730	617535	1	Integral membrane peptide transporter	Integral membrane peptide transporter; mediates transport of di- and tri-peptides; conserved protein that contains 12 transmembrane domains; PTR2 expression is regulated by N-end rule pathway via repression by Cup9p; targeted to vacuole via AP-3 pathway
S000001802	YKR094C	RPL40B	Ribosomal Protein of the Large subunit	617996	618750	-1	Ubiquitin-ribosomal 60S subunit protein L40B fusion protein	Ubiquitin-ribosomal 60S subunit protein L40B fusion protein; cleaved to yield ubiquitin and ribosomal protein L40B; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40B has a paralog, RPL40A, that arose from the whole genome duplication
S000001803	YKR095W	MLP1	Myosin-Like Protein	619805	625432	1	Myosin-like protein associated with the nuclear envelope	Myosin-like protein associated with the nuclear envelope; nuclear basket protein that connects the nuclear pore complex with the nuclear interior; involved with Tel1p in telomere length control; involved with Pml1p and Pml39p in nuclear retention of unspliced mRNAs; MLP1 has a paralog, MLP2, that arose from the whole genome duplication
S000001804	YKR096W	ESL2	EST/SMG-like	626793	630380	1	hEST1A/B (SMG5/6)-like protein	hEST1A/B (SMG5/6)-like protein; contributes to environment-sensing adaptive gene expression responses; Esl2p and Esl1p contain a 14-3-3-like domain and a putative PilT N-terminus ribonuclease domain; interacts with Pex14p; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; ESL2 has a paralog, ESL1, that arose from the whole genome duplication
S000001805	YKR097W	PCK1	Phosphoenolpyruvate CarboxyKinase	631152	632801	1	Phosphoenolpyruvate carboxykinase	Phosphoenolpyruvate carboxykinase; key enzyme in gluconeogenesis, catalyzes early reaction in carbohydrate biosynthesis, glucose represses transcription and accelerates mRNA degradation, regulated by Mcm1p and Cat8p, located in the cytosol
S000001806	YKR098C	UBP11	UBiquitin-specific Protease	633026	635179	-1	Ubiquitin-specific protease	Ubiquitin-specific protease; cleaves ubiquitin from ubiquitinated proteins; UBP11 has a paralog, UBP7, that arose from the whole genome duplication
S000001807	YKR099W	BAS1	BASal	635851	638286	1	Myb-related transcription factor	Myb-related transcription factor; involved in regulating basal and induced expression of genes of the purine and histidine biosynthesis pathways; controls cellular ATP levels; also involved in regulation of meiotic recombination at specific genes
S000001808	YKR100C	SKG1	Suppressor of lethality of Kex2 Gas1 double null mutant	638901	639968	-1	Transmembrane protein with a role in cell wall polymer composition	Transmembrane protein with a role in cell wall polymer composition; localizes on inner surface of plasma membrane at bud and in daughter cell; SKG1 has a paralog, AIM20, that arose from the whole genome duplication
S000001809	YKR101W	SIR1	Silent Information Regulator	640540	642504	1	Protein involved in silencing at mating-type loci HML and HMR	Protein involved in silencing at mating-type loci HML and HMR; recruitment to silent chromatin requires interactions with Orc1p and with Sir4p, through a common Sir1p domain; binds to centromeric chromatin
S000001810	YKR102W	FLO10	FLOcculation	646356	649865	1	Member of the FLO family of cell wall flocculation proteins	Member of the FLO family of cell wall flocculation proteins; not expressed in most lab strains; overproduction induces flocculation that can be inhibited by mannose, sucrose, or glucose; overproduction also promotes haploid invasive growth and diploid filamentous growth
S000001811	YKR103W	NFT1	New Full-length MRP-type Transporter	653080	656736	1	Putative transporter of the MRP subfamily	Putative transporter of the MRP subfamily; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds; MRP stands for multidrug resistance-associated protein
S000001812	YKR104W	""	""	656836	657756	1	Putative transporter of the MRP subfamily	Putative transporter of the MRP subfamily; contains a stop codon in S288C; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds; MRP stands for multidrug resistance-associated protein
S000001813	YKR105C	VBA5	Vacuolar Basic Amino acid transporter	658716	660464	-1	Plasma membrane protein of the Major Facilitator Superfamily (MFS)	Plasma membrane protein of the Major Facilitator Superfamily (MFS); involved in amino acid uptake and drug sensitivity; VBA5 has a paralog, VBA3, that arose from a segmental duplication
S000001814	YKR106W	GEX2	Glutathione EXchanger	661442	663289	1	Proton:glutathione antiporter	Proton:glutathione antiporter; localized to the vacuolar and plasma membranes; expressed at a very low level; potential role in resistance to oxidative stress and modulation of the PKA pathway; GEX2 has a paralog, GEX1, that arose from a segmental duplication
S000001817	YAL065C	""	""	11565	11951	-1	Putative protein of unknown function	Putative protein of unknown function; shows sequence similarity to FLO1 and other flocculins
S000001821	YAR030C	""	""	186512	186853	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YAR029W and the verified gene PRM9
S000001838	YFL056C	AAD6	Aryl-Alcohol Dehydrogenase	14305	15431	-1	Putative aryl-alcohol dehydrogenase	Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; ORFs AAD6/YFL056C and AAD16/YFL057C are displaced from one another by -1 frameshift; members of the AAD gene family comprise three pairs (AAD3 + AAD15, AAD6/AAD16 + AAD4, AAD10 + AAD14) whose two genes are more related to one another than to other members of the family; expression induced in cells treated with the mycotoxin patulin
S000001839	YFL055W	AGP3	high-Affinity Glutamine Permease	17004	18680	1	Low-affinity amino acid permease	Low-affinity amino acid permease; may act to supply the cell with amino acids as nitrogen source in nitrogen-poor conditions; transcription is induced under conditions of sulfur limitation; plays a role in regulating Ty1 transposition
S000001840	YFL054C	AQY3	AQuaporin from Yeast	20847	22787	-1	Putative channel-like protein	Putative channel-like protein; similar to Fps1p; mediates passive diffusion of glycerol in the presence of ethanol
S000001841	YFL053W	DAK2	DihydroxyAcetone Kinase	23423	25198	1	Dihydroxyacetone kinase	Dihydroxyacetone kinase; required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation
S000001842	YFL052W	ZNF1	ZiNc Finger protein	28232	29629	1	Zinc cluster transcription factor that regulates respiratory growth	Zinc cluster transcription factor that regulates respiratory growth; binds to promoters of genes involved in respiration, gluconeogenesis, and the glyoxylate shunt; required for normal carbon source utilization and adaptation to pH, osmotic and ethanol stress especially during non-fermentative metabolism
S000001843	YFL051C	""	""	30058	30540	-1	Putative protein of unknown function	Putative protein of unknown function; SWAT-GFP fusion protein localizes to the cell periphery while mCherry fusion protein localizes to both the cell periphery and vacuole; YFL051C is not an essential gene
S000001844	YFL050C	ALR2	ALuminum Resistance	33272	35848	-1	Probable Mg(2+) transporter	Probable Mg(2+) transporter; overexpression confers increased tolerance to Al(3+) and Ga(3+) ions; plays a role in regulating Ty1 transposition
S000001845	YFL049W	SWP82	SWi/snf-associated Protein	36803	38674	1	Member of the SWI/SNF chromatin remodeling complex	Member of the SWI/SNF chromatin remodeling complex; has an as yet unidentified role in the complex; has identifiable counterparts in closely related yeast species; abundantly expressed in many growth conditions; paralog of Npl6p; relocates to the cytosol under hypoxic conditions
S000001846	YFL048C	EMP47	""	38843	40180	-1	Integral membrane component of ER-derived COPII-coated vesicles	Integral membrane component of ER-derived COPII-coated vesicles; functions in ER to Golgi transport; forms a complex with Ssp120p that may function in trafficking plasma membrane glycoproteins through early secretory pathway; EMP47 has a paralog, EMP46, that arose from the whole genome duplication
S000001847	YFL047W	RGD2	RhoGAP domain	40421	42565	1	GTPase-activating protein (RhoGAP) for Cdc42p and Rho5p	GTPase-activating protein (RhoGAP) for Cdc42p and Rho5p; relocalizes from bud neck to cytoplasm upon DNA replication stress
S000001848	YFL046W	FMP32	Found in Mitochondrial Proteome	42815	43438	1	Regulator of mitochondrial proline metabolism	Regulator of mitochondrial proline metabolism; tethered with Put7p to inner mitochondrial membrane in large hetero-oligomeric complex, abundance of which is regulated by proline; involved in mitochondrial proline homeostasis and cellular redox balance; null exhibits pronounced defect in proline utilization, and can be functionally complemented by expression of human homolog MCUR1
S000001850	YFL044C	OTU1	Ovarian TUmor	44655	45560	-1	Deubiquitylation enzyme that binds to the chaperone-ATPase Cdc48p	Deubiquitylation enzyme that binds to the chaperone-ATPase Cdc48p; may contribute to regulation of protein degradation by deubiquitylating substrates that have been ubiquitylated by Ufd2p; member of the Ovarian Tumor (OTU) family; protein abundance increases in response to DNA replication stress
S000001851	YFL043C	""	""	45720	46157	-1	Merged open reading frame	Merged open reading frame; does not encode a discrete protein; YFL043C was originally annotated as an independent ORF, but as a result of a sequence change, it was merged with an adjacent ORF into a single reading frame, designated YFL042C
S000001852	YFL042C	LAM5	Lipid transfer protein Anchored at Membrane contact site	45720	47744	-1	Putative sterol transfer protein	Putative sterol transfer protein; one of six StART-like domain-containing proteins in yeast that may be involved in sterol transfer between intracellular membranes; conserved across eukaryotes; has both GRAM and StART-like (VASt) domains; localizes to membrane contact sites throughout the cell, including nucleus-vacuole junctions and ER-mitochondrial contact sites
S000001853	YFL041W	FET5	FErrous Transport	49139	51007	1	Multicopper oxidase	Multicopper oxidase; integral membrane protein with similarity to Fet3p; targeted to vacuole via AP-3 pathway; may have role in iron transport
S000001854	YFL040W	""	""	51350	52972	1	Putative transporter	Putative transporter; member of the sugar porter family; YFL040W is not an essential gene; may have a role in intracellular sterol transport
S000001856	YFL038C	YPT1	Yeast Protein Two	55366	55986	-1	Rab family GTPase	Rab family GTPase; involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by type II protein geranylgeranyltransferase (Bet2p-Bet4p); binds to unspliced HAC1 mRNA; regulates the unfolded protein response (UPR) by promoting the decay of HAC1 RNA; localizes to the early Golgi, the transitional Golgi and ER membranes, pre-autophagosomal structures, and cytoplasmic vesicles
S000001857	YFL037W	TUB2	TUBulin	56336	57709	1	Beta-tubulin	Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria
S000001858	YFL036W	RPO41	RNA POlymerase	58782	62837	1	Mitochondrial RNA polymerase	Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation; Rpo41p also synthesizes RNA primers for mitochondrial DNA replication
S000001860	YFL034W	MIL1	Medium adaptin-Interacting Ligand	65477	68698	1	Predicted lipase	Predicted lipase; binds variant medium clathrin chain Apm2p and contributes to its membrane recruitment; putative integral membrane protein that interacts with Rpp0p component of ribosomal stalk
S000001861	YFL033C	RIM15	Regulator of IME2	69115	74427	-1	Protein kinase involved in cell proliferation in response to nutrients	Protein kinase involved in cell proliferation in response to nutrients; glucose-repressible; involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; phosphorylates Igo1p and Igo2p; substrate of Pho80p-Pho85p kinase
S000001862	YFL032W	""	""	74872	75192	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene
S000001863	YFL031W	HAC1	Homologous to Atf/Creb1	75179	76147	1	Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog)	Basic leucine zipper (bZIP) transcription factor (ATF/CREB1 homolog); regulates unfolded protein response via UPRE binding and membrane biogenesis; translation initiation repressed under non-stress conditions by un-spliced intron that interacts with 5'-UTR; under ER stress Ire1p nuclease cleaves out inhibitory intron and triggers efficient Hac1p translation; two functional forms of Hac1p are produced; protein abundance increases in response to DNA replication stress
S000001864	YFL030W	AGX1	Alanine:Glyoxylate aminotrans(X)ferase	76831	77988	1	Alanine:glyoxylate aminotransferase (AGT)	Alanine:glyoxylate aminotransferase (AGT); catalyzes the synthesis of glycine from glyoxylate, which is one of three pathways for glycine biosynthesis in yeast; similar to mammalian and plant alanine:glyoxylate aminotransferases; human homolog AGXT complements yeast null mutant
S000001866	YFL028C	CAF16	CCR4 Associated Factor	79344	80213	-1	Part of evolutionarily-conserved CCR4-NOT regulatory complex	Part of evolutionarily-conserved CCR4-NOT regulatory complex; contains single ABC-type ATPase domain but no transmembrane domain; interacts with several subunits of Mediator
S000001867	YFL027C	GYP8	Gtpase-activating protein for Ypt Proteins	80419	81912	-1	GTPase-activating protein for yeast Rab family members	GTPase-activating protein for yeast Rab family members; Ypt1p is the preferred in vitro substrate but also acts on Sec4p, Ypt31p and Ypt32p; involved in the regulation of ER to Golgi vesicle transport; localizes to the ER and peroxisomes
S000001868	YFL026W	STE2	STErile	82580	83875	1	Receptor for alpha-factor pheromone	Receptor for alpha-factor pheromone; seven transmembrane-domain GPCR that interacts with both pheromone and a heterotrimeric G protein to initiate the signaling response that leads to mating between haploid a and alpha cells
S000001869	YFL025C	BST1	Bypass of Sec Thirteen	84145	87234	-1	GPI inositol deacylase of the endoplasmic reticulum (ER)	GPI inositol deacylase of the endoplasmic reticulum (ER); negatively regulates COPII vesicle formation; prevents production of vesicles with defective subunits; required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules; functional ortholog of human PGAP1, mutation of which is associated with intellectual disability and encephalopathy
S000001870	YFL024C	EPL1	Enhancer of Polycomb Like	87847	90345	-1	Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex	Subunit of NuA4, an essential histone H4/H2A acetyltransferase complex; conserved region at N-terminus is essential for interaction with the NPC (nucleosome core particle); required for autophagy; homologous to Drosophila Enhancer of Polycomb; coding sequence contains length polymorphisms in different strains
S000001871	YFL023W	BUD27	BUD site selection	90986	93376	1	Unconventional prefoldin protein involved in translation initiation	Unconventional prefoldin protein involved in translation initiation; required for correct assembly of RNAP I, II, and III in an Rpb5p-dependent manner; shuttles between nucleus and cytoplasm; mutants have inappropriate expression of nutrient sensitive genes due to translational derepression of Gcn4p transcription factor; diploid mutants show random budding; mutations affect TOR signaling; ortholog of human URI/RMP
S000001872	YFL022C	FRS2	phenylalanyl (F)-tRNA Synthetase	93499	95010	-1	Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase	Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase; forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar
S000001873	YFL021W	GAT1	""	95966	97498	1	Transcriptional activator of nitrogen catabolite repression genes	Transcriptional activator of nitrogen catabolite repression genes; contains a GATA-1-type zinc finger DNA-binding motif; activity and localization regulated by nitrogen limitation and Ure2p; different translational starts produce two major and two minor isoforms that are differentially regulated and localized
S000001874	YFL020C	PAU5	seriPAUperin family	99231	99599	-1	Member of the seripauperin multigene family	Member of the seripauperin multigene family; encoded mainly in subtelomeric regions; induced during alcoholic fermentation; induced by low temperature and also by anaerobic conditions; negatively regulated by oxygen and repressed by heme
S000001875	YFL019C	""	""	100252	100605	-1	Putative protein of unknown function	Putative protein of unknown function; conserved across S. cerevisiae strains; YFL019C is not an essential gene
S000001876	YFL018C	LPD1	LiPoamide Dehydrogenase	101628	103127	-1	Dihydrolipoamide dehydrogenase	Dihydrolipoamide dehydrogenase; the lipoamide dehydrogenase component (E3) of the pyruvate dehydrogenase and 2-oxoglutarate dehydrogenase multi-enzyme complexes; PDH complex is concentrated in spots within the mitochondrial matrix, often near the ERMES complex and near peroxisomes; LPD1 has a paralog, IRC15, that arose from the whole genome duplication
S000001877	YFL017C	GNA1	GlucosamiNe-6-phosphate Acetyltransferase	103983	104462	-1	Glucosamine-6-phosphate acetyltransferase	Glucosamine-6-phosphate acetyltransferase; evolutionarily conserved; required for multiple cell cycle events including passage through START, DNA synthesis, and mitosis; involved in UDP-N-acetylglucosamine synthesis, forms GlcNAc6P from AcCoA
S000001878	YFL016C	MDJ1	Mitochondrial DnaJ	104701	106236	-1	Co-chaperone that stimulates HSP70 protein Ssc1p ATPase activity	Co-chaperone that stimulates HSP70 protein Ssc1p ATPase activity; involved in protein folding/refolding in the mitochodrial matrix; required for proteolysis of misfolded proteins; member of the HSP40 (DnaJ) family of chaperones
S000001879	YFL015C	""	""	106469	106963	-1	Putative protein of unknown function	Putative protein of unknown function; conserved across S. cerevisiae strains; partially overlaps dubious ORF YFL015W-A; YFL015C is not an essential gene
S000001880	YFL014W	HSP12	Heat Shock Protein	107256	107585	1	Plasma membrane protein involved in maintaining membrane organization	Plasma membrane protein involved in maintaining membrane organization; involved in maintaining organization during stress conditions; induced by heat shock, oxidative stress, osmostress, stationary phase, glucose depletion, oleate and alcohol; protein abundance increased in response to DNA replication stress and dietary restriction; regulated by the HOG and Ras-Pka pathways; required for dietary restriction-induced lifespan extension
S000001881	YFL013C	IES1	Ino Eighty Subunit	107852	109930	-1	Subunit of the INO80 chromatin remodeling complex	Subunit of the INO80 chromatin remodeling complex; relocalizes to the cytosol in response to hypoxia
S000001882	YFL012W	""	""	110647	111093	1	Putative protein of unknown function	Putative protein of unknown function; transcribed during sporulation; null mutant exhibits increased resistance to rapamycin
S000001883	YFL011W	HXT10	HeXose Transporter	112345	113985	1	Hexose transporter	Hexose transporter; expressed at low levels and expression is repressed by glucose
S000001884	YFL010C	WWM1	WW domain containing protein interacting with Metacaspase	115108	115743	-1	WW domain containing protein of unknown function	WW domain containing protein of unknown function; binds to Mca1p, a caspase-related protease that regulates H2O2-induced apoptosis; overexpression causes G1 phase growth arrest and clonal death that is suppressed by overexpression of MCA1
S000001885	YFL009W	CDC4	Cell Division Cycle	116145	118484	1	F-box protein required for both the G1/S and G2/M phase transitions	F-box protein required for both the G1/S and G2/M phase transitions; modular substrate specificity factor which associates with core SCF (Cdc53p, Skp1p and Hrt1p/Rbx1p) to form the SCFCdc4 complex; SCFCdc4 acts as a ubiquitin-protein ligase directing ubiquitination of cyclin-dependent kinase (CDK) phosphorylated substrates, such as: Sic1p, Far1p, Cdc6p, Clb6p, and Cln3p
S000001886	YFL008W	SMC1	Stability of MiniChromosomes	119429	123106	1	Subunit of the multiprotein cohesin complex	Subunit of the multiprotein cohesin complex; essential protein involved in chromosome segregation and in double-strand DNA break repair; SMC chromosomal ATPase family member, binds DNA with a preference for DNA with secondary structure
S000001887	YFL007W	BLM10	BLeoMycin resistance	123479	129910	1	Proteasome activator	Proteasome activator; binds the core proteasome (CP) and stimulates proteasome-mediated protein degradation by inducing gate opening; required for sequestering CP into proteasome storage granule (PSG) during quiescent phase and for nuclear import of CP in proliferating cells; required for resistance to bleomycin, may be involved in protecting against oxidative damage; similar to mammalian PA200
S000001888	YFL006W	""	""	129140	129904	1	Merged open reading frame	Merged open reading frame; does not encode a discrete protein; YFL006W was originally annotated as an independent ORF, but as a result of a sequence change, it was merged with an adjacent ORF into a single reading frame, designated YFL007W
S000001889	YFL005W	SEC4	SECretory	130334	130981	1	Rab family GTPase	Rab family GTPase; essential for vesicle-mediated exocytic secretion and autophagy; associates with the exocyst component Sec15p and may regulate polarized delivery of transport vesicles to the exocyst at the plasma membrane; exocyst association is negatively regulated via phosphorylation by Cdc5p during cytokinesis
S000001890	YFL004W	VTC2	Vacuolar Transporter Chaperone	131810	134296	1	Regulatory subunit of vacuolar transporter chaperone (VTC) complex	Regulatory subunit of vacuolar transporter chaperone (VTC) complex; involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion; important regulator of substrate invagination from the vacuolar membrane; targeted to vacuole via AP-3 pathway; VTC2 has a paralog, VTC3, that arose from the whole genome duplication
S000001891	YFL003C	MSH4	MutS Homolog	134521	137157	-1	Protein involved in meiotic recombination	Protein involved in meiotic recombination; required for normal levels of crossing over, colocalizes with Zip2p to discrete foci on meiotic chromosomes, has homology to bacterial MutS protein
S000001894	YFL002C	SPB4	Suppressor of PAB1	145114	146934	-1	Putative ATP-dependent RNA helicase	Putative ATP-dependent RNA helicase; nucleolar protein required for synthesis of 60S ribosomal subunits at a late step in the pathway; sediments with 66S pre-ribosomes in sucrose gradients
S000001895	YFL001W	DEG1	DEpressed Growth rate	147131	148459	1	tRNA:pseudouridine synthase	tRNA:pseudouridine synthase; introduces pseudouridines at position 38 or 39 in tRNA; also responsible for pseudouracil modification of some mRNAs; important for maintenance of translation efficiency and normal cell growth, localizes to both the nucleus and cytoplasm; non-essential for viability
S000001897	YFR001W	LOC1	LOCalization of mRNA	149110	149724	1	Nucleolar protein involved in the asymmetric localization of ASH1 mRNA	Nucleolar protein involved in the asymmetric localization of ASH1 mRNA; binds cooperatively with She2p to the E3 zip-code element in the 3'-UTR of the mRNA; constituent of 66S pre-ribosomal particles involved in pre-rRNA processing and nuclear export of the 60S subunit; may facilitate Rpl43p loading; required post-transcriptionally for efficient retrotransposition; absence results in decreased Ty1 Gag:GFP protein levels; relocalizes from nucleus to cytoplasm upon DNA replication stress
S000001898	YFR002W	NIC96	Nucleoporin-Interacting Component of 96 kDa	150016	152535	1	Linker nucleoporin component of the nuclear pore complex (NPC)	Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup57p, and Nup49p)
S000001899	YFR003C	YPI1	Yeast Phosphatase Inhibitor	152657	153124	-1	Regulatory subunit of the type I protein phosphatase (PP1) Glc7p	Regulatory subunit of the type I protein phosphatase (PP1) Glc7p; Glc7p participates in the regulation of a variety of metabolic processes including mitosis and glycogen metabolism; in vitro evidence suggests Ypi1p is an inhibitor of Glc7p while in vivo evidence suggests it is an activator; overproduction causes decreased cellular content of glycogen; partial depletion causes lithium sensitivity, while overproduction confers lithium-tolerance
S000001900	YFR004W	RPN11	Regulatory Particle Non-ATPase	153393	154313	1	Metalloprotease subunit of 19S regulatory particle	Metalloprotease subunit of 19S regulatory particle; part of 26S proteasome lid; couples the deubiquitination and degradation of proteasome substrates; involved, independent of catalytic activity, in fission of mitochondria and peroxisomes; protein abundance increases in response to DNA replication stress
S000001901	YFR005C	SAD1	SnRNP Assembly Defective	154527	155873	-1	Conserved zinc-finger domain protein involved in pre-mRNA splicing	Conserved zinc-finger domain protein involved in pre-mRNA splicing; critical for splicing of nearly all intron-containing genes; required for assembly of U4 snRNA into the U4/U6 particle
S000001902	YFR006W	""	""	156145	157752	1	Putative X-Pro aminopeptidase	Putative X-Pro aminopeptidase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YFR006W is not an essential gene
S000001903	YFR007W	YFH7	""	159299	160360	1	Putative kinase with similarity to the PRK/URK/PANK kinase subfamily	Putative kinase with similarity to the PRK/URK/PANK kinase subfamily; the PRK/URK/PANK subfamily of P-loop kinases is also known as phosphoribulokinase/uridine kinase/bacterial pantothenate kinase
S000001904	YFR008W	FAR7	Factor ARrest	160535	161200	1	Protein involved in recovery from pheromone-induced cell cycle arrest	Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress
S000001905	YFR009W	GCN20	General Control Nonderepressible	162488	164746	1	Positive regulator of the Gcn2p kinase activity	Positive regulator of the Gcn2p kinase activity; important for disaggregation of ordered and disordered protein aggregates; forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA; suppresses frameshifting at stalled ribosomes
S000001906	YFR010W	UBP6	UBiquitin-specific Protease	165067	166566	1	Ubiquitin-specific protease	Ubiquitin-specific protease; situated in the base subcomplex of the 26S proteasome, releases free ubiquitin from branched polyubiquitin chains en bloc, rather than from the distal tip of the chain; negatively regulates degradation of ubiquitinated proteins by the proteasome; works in opposition to Hul5p polyubiquitin elongation activity; mutant has aneuploidy tolerance; human homolog UBP14 complements yeast null mutant
S000001907	YFR011C	MIC19	MItochondrial contact site and Cristae organizing system	166746	167258	-1	Component of the MICOS complex	Component of the MICOS complex; MICOS (formerly MINOS or MitOS) is a mitochondrial inner membrane complex that extends into the intermembrane space and has a role in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane; Mic19p is peripheral to the inner membrane and may connect Mic60p with the Mic10p-Mic12p-Mic27p subcomplex; both yeast and human Mic19p become oxidized, and oxidation may regulate MICOS
S000001908	YFR012W	DCV1	Demands Cdc28 kinase activity for Viability	167888	168496	1	Protein of unknown function	Protein of unknown function; deletion mutant shows strong genetic interaction with cdc28-as1 mutant in the presence of 1-NM-PP1; DCV1 has a paralog, YOL019W, that arose from the whole genome duplication
S000001909	YFR013W	IOC3	Iswi One Complex	169922	172285	1	Subunit of the Isw1a complex	Subunit of the Isw1a complex; Isw1a has nucleosome-stimulated ATPase activity and represses transcription initiation by specific positioning of a promoter proximal dinucleosome; promotes nucleosome shifts in the 5 prime direction; IOC3 has a paralog, ESC8, that arose from the whole genome duplication
S000001910	YFR014C	CMK1	CalModulin dependent protein Kinase	172537	173877	-1	Calmodulin-dependent protein kinase	Calmodulin-dependent protein kinase; may play a role in stress response, many Ca++/calmodulin dependent phosphorylation substrates demonstrated in vitro, amino acid sequence similar to mammalian Cam Kinase II; CMK1 has a paralog, CMK2, that arose from the whole genome duplication
S000001911	YFR015C	GSY1	Glycogen SYnthase	174265	176391	-1	Glycogen synthase	Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, environmental stress, and entry into stationary phase; GSY1 has a paralog, GSY2, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
S000001912	YFR016C	AIP5	Actin Interacting Protein	177042	180743	-1	Protein that positively regulates actin assembly	Protein that positively regulates actin assembly; forms a homodimer and interacts with G-actin; localization to the bud neck and bud tip requires Spa2p; YFR016C is not an essential gene
S000001913	YFR017C	IGD1	Inhibitor of Glycogen Debranching	182274	182861	-1	Cytoplasmic protein that inhibits Gdb1p glycogen debranching activity	Cytoplasmic protein that inhibits Gdb1p glycogen debranching activity; required for normal intracellular accumulation of glycogen; phosphorylated in vivo; expression increases during wine fermentation; protein abundance increases in response to DNA replication stress; IGD1 has a paralog, YOL024W, that arose from the whole genome duplication
S000001914	YFR018C	""	""	183131	184222	-1	Putative protein of unknown function	Putative protein of unknown function; SWAT-GFP and seamless GFP fusion proteins localize to the endoplasmic reticulum and mCherry fusion protein localizes to the vacuole
S000001915	YFR019W	FAB1	Forms Aploid and Binucleate cells	184502	191338	1	1-phosphatidylinositol-3-phosphate 5-kinase	1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membrane kinase that generates phosphatidylinositol (3,5)P2, which is involved in vacuolar sorting and homeostasis
S000001916	YFR020W	CSS2	Condition Specific Secretion	192737	193435	1	Protein of unknown function, secreted when constitutively expressed	Protein of unknown function, secreted when constitutively expressed; SWAT-GFP fusion protein localizes to the endoplasmic reticulum (ER) and extracellular region, while mCherry fusion protein localizes to the ER and vacuole; mRNA identified as translated by ribosome profiling data; CSS2 is a non-essential gene
S000001917	YFR021W	ATG18	AuTophaGy related	194812	196314	1	Phosphoinositide binding protein	Phosphoinositide binding protein; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (CVT) pathway; binds PtdIns(3,5)P2, PI3P and PI4P; WD-40 repeat containing protein and PROPPIN family member; relocalizes from the vacuole to cytoplasm upon DNA replication stress; mammalian homologs include: WIPI1, WIPI2, WIPI3 and WIPI4/WDR45; mutations in human WDR45 cause static encephalopathy of childhood with neurodegeneration in adulthood (SENDA)
S000001918	YFR022W	ROG3	Revertant Of Glycogen synthase kinase mutation	196833	199034	1	Alpha-arrestin involved in ubiquitin-dependent endocytosis	Alpha-arrestin involved in ubiquitin-dependent endocytosis; contributes to desensitization of agonist-occupied alpha-factor receptor Ste2p by Rsp5p-independent internalization; PPXY motif-mediated binding of the ubiquitin ligase Rsp5p is not required for adaptation; mutation suppresses the temperature sensitivity of an mck1 rim11 double mutant; SWAT-GFP and mCherry fusion proteins localize to the cytosol; ROG3 has a paralog, ROD1, that arose from the whole genome duplication
S000001919	YFR023W	PES4	Polymerase Epsilon Suppressor	199874	201709	1	Putative RNA binding protein	Putative RNA binding protein; partially redundant function with paralog MIP6 in the steady-state expression, translational timing and localization of a subset of Ndt80-dependent mRNAs that are protected until translated near the end of meiosis II; regulator of meiotic commitment; redundant function with MIP6 in sporulation; localizes to puncta near the openings of the prospore membrane; suppressor of a DNA polymerase epsilon mutation; induced by NDT80
S000001920	YFR024C	""	""	201947	203068	-1	Deleted ORF	Deleted ORF; does not encode a discrete protein; YFR024C was originally annotated as a separate ORF, but was later removed because it was demonstrated to be theC-terminus of LSB3/YFR024C-A
S000001921	YFR025C	HIS2	HIStidine requiring	203743	204750	-1	Histidinolphosphatase	Histidinolphosphatase; catalyzes the eighth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control
S000001922	YFR026C	ULI1	Upr-L-Inducible gene	205748	206257	-1	Protein of unknown function	Protein of unknown function; involved in and induced by the endoplasmic reticulum unfolded protein response (UPR); SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum
S000001923	YFR027W	ECO1	Establishment of COhesion	207452	208297	1	Acetyltransferase required for sister chromatid cohesion	Acetyltransferase required for sister chromatid cohesion; elicits cohesin dimerization during S phase; acetylates Mps3p to regulate nuclear organization; modifies Smc3p at replication forks and Mcd1p in response to dsDNA breaks; phosphorylated by Cdc28p, Cdc7p, Mck1p to generate pair of phosphates spaced precisely for recognition by ubiquitin ligase SCF-Cdc4; relative distribution to nucleus increases upon DNA replication stress; mutations in human homolog ESCO2 cause Roberts syndrome
S000001924	YFR028C	CDC14	Cell Division Cycle	208413	210068	-1	Protein phosphatase required for mitotic exit	Protein phosphatase required for mitotic exit; required for rDNA segregation, cytokinesis, meiosis I spindle disassembly, environmental stress response, autophagy during meiosis; downregulates microautophagy induction after TORC1 inactivation; held in nucleolus by Cdc55p in early meiosis, liberated by FEAR and Mitotic Exit Network in anaphase; sequestered in metaphase II, released upon entry into anaphase II; human homolog CDC14A can complement thermosensitivity of cdc14-1 mutant
S000001925	YFR029W	PTR3	Peptide TRansport	210937	212973	1	Component of the SPS plasma membrane amino acid sensor system	Component of the SPS plasma membrane amino acid sensor system; senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes; other members are Ssy1p, Ptr3p, and Ssy5p
S000001926	YFR030W	MET10	METhionine requiring	213312	216419	1	Subunit alpha of assimilatory sulfite reductase	Subunit alpha of assimilatory sulfite reductase; complex converts sulfite into sulfide
S000001927	YFR031C	SMC2	Structural Maintenance of Chromosomes	216594	220106	-1	Subunit of the condensin complex	Subunit of the condensin complex; condensin reorganizes chromosomes during both mitosis and meiosis; essential SMC chromosomal ATPase family member that forms a subcomplex with Smc2p that has ATP-hydrolyzing and DNA-binding activity, but other condensin subunits are required for chromatin binding; required for clustering of tRNA genes at the nucleolus
S000001928	YFR032C	RRT5	Regulator of rDNA Transcription	222090	222959	-1	Putative protein of unknown function	Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; expressed at high levels during sporulation
S000001929	YFR033C	QCR6	ubiQuinol-cytochrome C oxidoReductase	224326	224769	-1	Subunit 6 of the ubiquinol cytochrome-c reductase complex	Subunit 6 of the ubiquinol cytochrome-c reductase complex; the complex, also known as the cytochrome bc(1) complex or Complex III, is a component of the mitochondrial inner membrane electron transport chain; highly acidic protein; required for maturation of cytochrome c1; may be loosely associated with the complex since it is easily released into the intermembrane space
S000001930	YFR034C	PHO4	PHOsphate metabolism	225020	225958	-1	Basic helix-loop-helix (bHLH) transcription factor of the myc-family	Basic helix-loop-helix (bHLH) transcription factor of the myc-family; activates transcription cooperatively with Pho2p in response to phosphate limitation; binding to 'CACGTG' motif is regulated by chromatin restriction, competitive binding of Cbf1p to the same DNA binding motif and cooperation with Pho2p; function is regulated by phosphorylation at multiple sites and by phosphate availability
S000001931	YFR035C	""	""	226121	226465	-1	Putative protein of unknown function	Putative protein of unknown function; deletion mutant exhibits synthetic phenotype with alpha-synuclein
S000001932	YFR036W	CDC26	Cell Division Cycle	226963	227337	1	Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C)	Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; relocalizes to the cytosol in response to hypoxia
S000001933	YFR037C	RSC8	Remodel the Structure of Chromatin	227513	229186	-1	Component of the RSC chromatin remodeling complex	Component of the RSC chromatin remodeling complex; essential for viability and mitotic growth; homolog of SWI/SNF subunit Swi3p, but unlike Swi3p, does not activate transcription of reporters
S000001934	YFR038W	IRC5	Increased Recombination Centers	229380	231941	1	Putative ATPase containing the DEAD/H helicase-related sequence motif	Putative ATPase containing the DEAD/H helicase-related sequence motif; null mutant displays increased levels of spontaneous Rad52p foci; SWAT-GFP and mCherry fusion proteins localize to the nucleus
S000001935	YFR039C	OSW7	Outer Spore Wall	232012	233544	-1	Protein involved in outer spore wall assembly	Protein involved in outer spore wall assembly; likely involved directly in dityrosine layer assembly; may be involved in response to high salt and changes in carbon source; SWAT-GFP, seamless-GFP and mCherry fusion proteins localize to the endoplasmic reticulum; deletion mutant has decreased spore survival in Drosophila feces; OSW7 has a paralog, SHE10, that arose from the whole genome duplication; paralogs are redundant for spore wall dityrosine assembly
S000001936	YFR040W	SAP155	Sit4 Associated Protein	234242	237250	1	Protein required for function of the Sit4p protein phosphatase	Protein required for function of the Sit4p protein phosphatase; forms a complex with Sit4p; member of a family of similar proteins including Sap4p, Sap185p, and Sap190p; protein abundance increases in response to DNA replication stress; SAP155 has a paralog, SAP4, that arose from the whole genome duplication
S000001937	YFR041C	ERJ5	Endoplasmic Reticulum located J-protein	237368	238255	-1	Type I membrane protein with a J domain	Type I membrane protein with a J domain; required to preserve the folding capacity of the endoplasmic reticulum; loss of the non-essential ERJ5 gene leads to a constitutively induced unfolded protein response
S000001939	YFR043C	IRC6	Increased Recombination Centers	239114	239827	-1	Clathrin coat accessory factor	Clathrin coat accessory factor; involved in clathrin-mediated vesicle trafficking; may function to link the AP-1 clathrin adaptor complex with the Rab GTPase Ypt31p; has structural similarity to G-proteins; mouse homolog Aagab (p34) functionally complements irc6 null mutation; null mutant displays increased levels of spontaneous Rad52p foci
S000001940	YFR044C	DUG1	Deficient in Utilization of Glutathione	239992	241437	-1	Cys-Gly metallo-di-peptidase	Cys-Gly metallo-di-peptidase; forms a complex with Dug2p and Dug3p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p); human homolog CNDP2 can complement yeast dug1 mutant
S000001941	YFR045W	MRX20	Mitochondrial oRganization of gene eXpression (MIOREX)	241998	242999	1	Putative mitochondrial transport protein	Putative mitochondrial transport protein; null mutant is viable, exhibits decreased levels of chitin and normal resistance to calcofluor white
S000001942	YFR046C	CNN1	Co-purified with NNf1p	243074	244159	-1	Kinetochore protein	Kinetochore protein; associated with the essential kinetochore proteins Nnf1p and Spc24p; phosphorylated by Clb5-Cdk1, Mps1p, Ipl1p and to a lesser extent by Clb2-Cdk1; localizes to the lower region of the Ndc80 complex during anaphase and regulates KMN activity by inhibiting the Mtw1 and Spc105 complexes from binding to the Ndc80 complex; similar to metazoan CENP-T
S000001943	YFR047C	BNA6	Biosynthesis of Nicotinic Acid	244279	245166	-1	Quinolinate phosphoribosyl transferase	Quinolinate phosphoribosyl transferase; required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p
S000001944	YFR048W	RMD8	Required for Meiotic nuclear Division	246146	248134	1	Cytosolic protein required for sporulation	Cytosolic protein required for sporulation
S000001945	YFR049W	YMR31	Yeast Mitochondrial Ribosomal protein	248523	248894	1	Subunit of the mitochondrial alpha-ketoglutarate dehydrogenase	Subunit of the mitochondrial alpha-ketoglutarate dehydrogenase; recruits E3 subunit (Lpd1p) to the E1-E2 (Kgd1p, Kgd2p) core; exists in two forms derived from a single mRNA translated from two alternative start sites; has similarity to human mitochondrial ribosomal protein MRP-S36
S000001946	YFR050C	PRE4	PRoteinase yscE	249066	249866	-1	Beta 7 subunit of the 20S proteasome	Beta 7 subunit of the 20S proteasome
S000001947	YFR051C	RET2	RETrieval from ER	250163	251803	-1	Delta subunit of the coatomer complex (COPI)	Delta subunit of the coatomer complex (COPI); COPI coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER
S000001948	YFR052W	RPN12	Regulatory Particle Non-ATPase	252505	253329	1	Subunit of the 19S regulatory particle of the 26S proteasome lid	Subunit of the 19S regulatory particle of the 26S proteasome lid; synthetically lethal with RPT1, which is an ATPase component of the 19S regulatory particle; physically interacts with Nob1p and Rpn3p; protein abundance increases in response to DNA replication stress
S000001949	YFR053C	HXK1	HeXoKinase	253592	255049	-1	Hexokinase isoenzyme 1	Hexokinase isoenzyme 1; a cytosolic protein that catalyzes phosphorylation of glucose during glucose metabolism; expression is highest during growth on non-glucose carbon sources; glucose-induced repression involves hexokinase Hxk2p; HXK1 has a paralog, HXK2, that arose from the whole genome duplication
S000001950	YFR054C	""	""	258855	259433	-1	Putative protein of unknown function	Putative protein of unknown function; conserved among S. cerevisiae strains
S000001951	YFR056C	""	""	263957	264325	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YFR055W
S000001952	YFR055W	IRC7	Increased Recombination Centers	264204	265226	1	Cysteine desulphydrase, enables growth on cysteine as nitrogen source	Cysteine desulphydrase, enables growth on cysteine as nitrogen source; involved in the production of thiols; null mutant displays increased levels of spontaneous Rad52p foci; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner and by copper levels in a Mac1-dependent manner
S000001953	YFR057W	""	""	269061	269516	1	Telomere-linked gene, silencing of which is strictly Sir2-dependent	Telomere-linked gene, silencing of which is strictly Sir2-dependent
S000001954	YER044C-A	MEI4	MEIosis-specific	238460	239774	-1	Meiosis-specific protein involved in forming DSBs	Meiosis-specific protein involved in forming DSBs; involved in double-strand break (DSBs) formation during meiotic recombination; required for chromosome synapsis and production of viable spores
S000001955	YFL010W-A	AUA1	Amino acid Uptake Activation	114990	115274	1	Protein required for the negative regulation by ammonia of Gap1p	Protein required for the negative regulation by ammonia of Gap1p; Gap1p is a general amino acid permease
S000001956	YKL096W-A	CWP2	Cell Wall Protein	259253	259531	1	Covalently linked cell wall mannoprotein	Covalently linked cell wall mannoprotein; major constituent of the cell wall; plays a role in stabilizing the cell wall; involved in low pH resistance; precursor is GPI-anchored
S000001957	YHR079C-A	SAE3	Sporulation in the Absence of spo Eleven	262192	262553	-1	Meiosis-specific protein involved in meiotic recombination	Meiosis-specific protein involved in meiotic recombination; involved in DMC1-dependent meiotic recombination; forms heterodimer with Mei5p; proposed to be an assembly factor for Dmc1p
S000002100	YHR039C-A	VMA10	""	187173	187679	-1	Subunit G of the V1 peripheral membrane domain of V-ATPase	Subunit G of the V1 peripheral membrane domain of V-ATPase; part of the electrogenic proton pump found throughout the endomembrane system; involved in vacuolar acidification; the V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase) has eight subunits
S000002103	YEL017C-A	PMP2	Plasma Membrane Proteolipid	122798	122929	-1	Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p)	Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; protein abundance increases in response to DNA replication stress; PMP2 has a paralog, PMP1, that arose from the whole genome duplication
S000002104	YFR031C-A	RPL2A	Ribosomal Protein of the Large subunit	220507	221418	-1	Ribosomal 60S subunit protein L2A	Ribosomal 60S subunit protein L2A; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2A has a paralog, RPL2B, that arose from the whole genome duplication
S000002127	YER019C-A	SBH2	Sec61 beta homolog	194273	194539	-1	Ssh1p-Sss1p-Sbh2p complex component	Ssh1p-Sss1p-Sbh2p complex component; involved in protein translocation into the endoplasmic reticulum; SBH2 has a paralog, SBH1, that arose from the whole genome duplication
S000002128	YER087C-B	SBH1	Sec61 beta homolog	332582	332830	-1	Beta subunit of Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p)	Beta subunit of Sec61p ER translocation complex (Sec61p-Sss1p-Sbh1p); involved in protein translocation into the endoplasmic reticulum; interacts with the exocyst complex and also with Rtn1p; cotranslationally N-acetylated by NatA; SBH1 has a paralog, SBH2, that arose from the whole genome duplication
S000002129	YAR002C-A	ERP1	Emp24p/Erv25p Related Protein	154065	154724	-1	Member of the p24 family involved in ER to Golgi transport	Member of the p24 family involved in ER to Golgi transport; role in misfolded protein quality control; forms heterotrimeric complex with Erp2p, Emp24p, and Erv25p; localized to COPII-coated vesicles; ERP1 has a paralog, ERP6, that arose from the whole genome duplication
S000002133	YCR079W	PTC6	Phosphatase Two C	252845	254173	1	Mitochondrial type 2C protein phosphatase (PP2C)	Mitochondrial type 2C protein phosphatase (PP2C); has similarity to mammalian PP1Ks; involved in mitophagy; null mutant is sensitive to rapamycin and has decreased phosphorylation of the Pda1 subunit of pyruvate dehydrogenase
S000002134	YAL034C	FUN19	Function Unknown Now	80710	81951	-1	Non-essential protein of unknown function	Non-essential protein of unknown function; expression induced in response to heat stress; FUN19 has a paralog, YOR338W, that arose from the whole genome duplication
S000002135	YER056C-A	RPL34A	Ribosomal Protein of the Large subunit	269423	270185	-1	Ribosomal 60S subunit protein L34A	Ribosomal 60S subunit protein L34A; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34A has a paralog, RPL34B, that arose from the whole genome duplication
S000002136	YAL004W	""	""	140760	141407	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps verified gene SSA1/YAL005C
S000002138	YAL042C-A	""	""	61231	61608	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified ORF ERV46/YAL042W; YAL042C-A is a non-essential gene
S000002139	YAL056C-A	""	""	38696	39046	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000002140	YAL064C-A	TDA8	Topoisomerase I Damage Affected	13363	13743	-1	Putative protein of unknown function	Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene
S000002142	YAL068C	PAU8	seriPAUperin	1807	2169	-1	Protein of unknown function	Protein of unknown function; member of the seripauperin multigene family encoded mainly in subtelomeric regions
S000002156	YBR084C-A	RPL19A	Ribosomal Protein of the Large subunit	414186	415261	-1	Ribosomal 60S subunit protein L19A	Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication
S000002157	YBR089C-A	NHP6B	Non-Histone Protein	426190	426489	-1	High-mobility group (HMG) protein	High-mobility group (HMG) protein; binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to the chromosomes; functionally redundant with Nhp6Ap; required for transcriptional initiation fidelity of some tRNA genes; homologous to mammalian HMGB1 and HMGB2; NHP6B has a paralog, NHP6A, that arose from the whole genome duplication
S000002158	YBR162W-A	YSY6	""	565231	565428	1	Protein of unknown function	Protein of unknown function; expression suppresses a secretory pathway mutation in E. coli; has similarity to the mammalian RAMP4 protein involved in secretion
S000002159	YDL001W	RMD1	Required for Meiotic nuclear Division	447984	449276	1	Cytoplasmic protein required for sporulation	Cytoplasmic protein required for sporulation; targeted to vacuole via AP-3 pathway
S000002160	YDL002C	NHP10	Non-Histone Protein	446967	447578	-1	Non-essential INO80 chromatin remodeling complex subunit	Non-essential INO80 chromatin remodeling complex subunit; preferentially binds DNA ends, protecting them from exonucleatic cleavage; deletion affects telomere maintenance via recombination; related to mammalian high mobility group proteins
S000002161	YDL003W	MCD1	Mitotic Chromosome Determinant	444683	446383	1	Essential alpha-kleisin subunit of the cohesin complex	Essential alpha-kleisin subunit of the cohesin complex; required for sister chromatid cohesion in mitosis; subject to proteolytic cleavage by separase Esp1p, resulting in dissociation of cohesin from chromatin and the separation of sister chromatids at the mitotic metaphase-to-anaphase transition; apoptosis induces cleavage and translocation of a C-terminal fragment to mitochondria; expression peaks in S phase
S000002162	YDL004W	ATP16	ATP synthase	443029	443511	1	Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase	Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; F1 translationally regulates ATP6 and ATP8 expression to achieve a balanced output of ATP synthase genes encoded in nucleus and mitochondria; phosphorylated
S000002164	YDL006W	PTC1	Phosphatase type Two C	439909	440754	1	Type 2C protein phosphatase (PP2C)	Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p, inactivating the osmosensing MAPK cascade; involved in Fus3p activation during pheromone response; deletion affects precursor tRNA splicing, mitochondrial inheritance, and sporulation; involved in myosin-V dependent inheritance of mitochondria, peroxisomes, vacuoles and secretory vesicles; localizes to the nucleus, cytoplasm and peroxisomes
S000002165	YDL007W	RPT2	Regulatory Particle Triple-A protein, or Regulatory Particle Triphosphatase	438047	439360	1	ATPase of the 19S regulatory particle of the 26S proteasome	ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle; N-myristoylation of Rpt2p at Gly2 is involved in regulating the proper intracellular distribution of proteasome activity by controlling the nuclear localization of the 26S proteasome
S000002166	YDL008W	APC11	Anaphase Promoting Complex	433497	433994	1	Catalytic core subunit, Anaphase-Promoting Complex/Cyclosome (APC/C)	Catalytic core subunit, Anaphase-Promoting Complex/Cyclosome (APC/C); which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; contains a RING-H2 domain that is required for activity
S000002167	YDL009C	""	""	432925	433248	-1	Protein of unknown function	Protein of unknown function; mRNA identified as translated by ribosome profiling data; SWAT-GFP and mCherry fusion proteins localize to the cytosol; partially overlaps the verified ORF YDL010W; YDL009C is not an essential gene
S000002168	YDL010W	GRX6	GlutaRedoXin	432330	433025	1	Cis-Golgi localized monothiol glutaredoxin, binds Fe-S cluster	Cis-Golgi localized monothiol glutaredoxin, binds Fe-S cluster; more similar in activity to dithiol than other monothiol glutaredoxins; involved in the oxidative stress response; GRX6 has a paralog, GRX7, that arose from the whole genome duplication
S000002169	YDL011C	""	""	432308	432631	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the uncharacterized ORF YDL010W
S000002170	YDL012C	""	""	431108	431517	-1	Tail-anchored plasma membrane protein with a conserved CYSTM module	Tail-anchored plasma membrane protein with a conserved CYSTM module; possibly involved in response to stress; may contribute to non-homologous end-joining (NHEJ) based on ydl012c htz1 double null phenotype; YDL012C has a paralog, YBR016W, that arose from the whole genome duplication
S000002171	YDL013W	SLX5	Synthetic Lethal of unknown [X] function	429067	430926	1	Subunit of the Slx5-Slx8 SUMO-targeted Ub ligase (STUbL) complex	Subunit of the Slx5-Slx8 SUMO-targeted Ub ligase (STUbL) complex; role in Ub-mediated degradation of histone variant Cse4p preventing mislocalization to euchromatin; role in proteolysis of spindle positioning protein Kar9p, and DNA repair proteins Rad52p and Rad57p; forms SUMO-dependent nuclear foci, including DNA repair centers; contains a RING domain and two SIM motifs; associates with the centromere; required for maintenance of genome integrity like human ortholog RNF4
S000002172	YDL014W	NOP1	NucleOlar Protein	427364	428347	1	Histone glutamine methyltransferase, modifies H2A at Q105 in nucleolus	Histone glutamine methyltransferase, modifies H2A at Q105 in nucleolus; component of the small subunit processome complex, which is required for processing of pre-18S rRNA; ortholog of mammalian fibrillarin; inviability of the null mutant is functionally complemented by human FBL
S000002173	YDL015C	TSC13	Temperature-sensitive Suppressors of Csg2 mutants	426002	426934	-1	Enoyl reductase	Enoyl reductase; catalyzes last step in each cycle of very long chain fatty acid elongation; localizes to ER, highly enriched in a structure marking nuclear-vacuolar junctions; coimmunoprecipitates with elongases Elo2p and Elo3p; protein increases in abundance and relative distribution to ER foci increases upon DNA replication stress; human homolog TECR implicated in nonsyndromic mental retardation, can complement yeast mutant
S000002175	YDL017W	CDC7	Cell Division Cycle	424209	425732	1	S/T kinase and catalytic subunit of DDK (Dbf4-dependent kinase)	S/T kinase and catalytic subunit of DDK (Dbf4-dependent kinase); required for origin firing and replication initiation; phosphorylates MCM subunits and Cdc45 to drive assembly of the pre-IC and the CMG helicase; maintains Cse4p at kinetochores through phosphorylation to regulate chromosome segregation, regulates pre-meiotic replication, meiotic DSB formation, monopolar attachment of homologs during MI through monopolin recruitment and middle meiotic gene expression; HsCDC7 and DBF4 complement
S000002176	YDL018C	ERP3	Emp24p/Erv25p Related Protein	422833	423510	-1	Protein with similarity to Emp24p and Erv25p	Protein with similarity to Emp24p and Erv25p; member of the p24 family involved in ER to Golgi transport
S000002177	YDL019C	OSH2	OxySterol binding protein Homolog	417663	421514	-1	Member of an oxysterol-binding protein family with seven members	Member of an oxysterol-binding protein family with seven members; in S. cerevisiae, family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability; contains FFAT motif; interacts with ER anchor Scs2p at patches at the plasma membrane and at the nuclear envelope; regulated by sterol binding; OSH2 has a paralog, SWH1, that arose from the whole genome duplication
S000002178	YDL020C	RPN4	Regulatory Particle Non-ATPase	415113	416708	-1	Transcription factor that stimulates expression of proteasome genes	Transcription factor that stimulates expression of proteasome genes; Rpn4p levels are in turn regulated by the 26S proteasome in a negative feedback control mechanism; RPN4 is transcriptionally regulated by various stress responses; relative distribution to the nucleus increases upon DNA replication stress
S000002179	YDL021W	GPM2	Glycerate PhosphoMutase	413953	414888	1	Nonfunctional homolog of Gpm1p phosphoglycerate mutase	Nonfunctional homolog of Gpm1p phosphoglycerate mutase; if functional, would convert 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; GPM2 has a paralog, GPM3, that arose from the whole genome duplication
S000002180	YDL022W	GPD1	Glycerol-3-Phosphate Dehydrogenase	411825	413000	1	NAD-dependent glycerol-3-phosphate dehydrogenase	NAD-dependent glycerol-3-phosphate dehydrogenase; key enzyme of glycerol synthesis, essential for growth under osmotic stress; expression regulated by high-osmolarity glycerol response pathway; protein abundance increases in response to DNA replication stress; constitutively inactivated via phosphorylation by the protein kinases Ypk1p and Ypk2p, dephosphorylation increases catalytic activity; forms a heterodimer with Pnc1p to facilitate its peroxisomal import
S000002181	YDL023C	""	""	411761	412081	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in other Saccharomyces species; overlaps the verified gene GPD1; deletion confers sensitivity to GSAO; deletion in cyr1 mutant results in loss of stress resistance
S000002182	YDL024C	DIA3	Digs Into Agar	408451	409857	-1	Protein of unknown function	Protein of unknown function; involved in invasive and pseudohyphal growth
S000002183	YDL025C	RTK1	Ribosome biogenesis and TRNA synthetase-associated Kinase	405344	407206	-1	Putative protein kinase, potentially phosphorylated by Cdc28p	Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress
S000002184	YDL026W	""	""	404749	405060	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000002185	YDL027C	MRX9	Mitochondrial oRganization of gene eXpression (MIOREX)	403695	404957	-1	Mitochondrial inner membrane protein	Mitochondrial inner membrane protein; high copy suppressor of ER stress-mediated cell death; overexpression increases O2 consumption, impairs processing of COB and COX1 RNAs encoding mitochondrial oxidative phosphorylation factors, and impacts the transition from fermentative to respiratory metabolism
S000002186	YDL028C	MPS1	MonoPolar Spindle	400997	403291	-1	Dual-specificity kinase	Dual-specificity kinase; required for SPB duplication and spindle checkpoint function in mitosis and meiosis; contributes to bi-orientation, promoting formation of force-generating kinetochore-microtubule attachments in meiosis I; autophosphorylation required for function; substrates include SPB proteins (Spc42p, Spc110p, Spc98p), mitotic exit network protein Mob1p, kinetochore protein Cnn1p, and checkpoint protein Mad1p; substrate of APCC(Cdh1); detected in peroxisomes; similar to human Mps1p
S000002187	YDL029W	ARP2	Actin-Related Protein	399340	400638	1	Essential component of the Arp2/3 complex	Essential component of the Arp2/3 complex; Arp2/3 is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity; required for efficient Golgi-to-ER trafficking in COPI mutants
S000002188	YDL030W	PRP9	Pre-mRNA Processing	397537	399129	1	Subunit of the SF3a splicing factor complex	Subunit of the SF3a splicing factor complex; required for spliceosome assembly; acts after the formation of the U1 snRNP-pre-mRNA complex
S000002189	YDL031W	DBP10	Dead Box Protein	394217	397204	1	Putative ATP-dependent RNA helicase of the DEAD-box protein family	Putative ATP-dependent RNA helicase of the DEAD-box protein family; constituent of 66S pre-ribosomal particles; essential protein involved in ribosome biogenesis
S000002190	YDL032W	""	""	393690	394001	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified gene SLM3/YDL033C; YDL032W is not an essential gene
S000002191	YDL033C	SLM3	Synthetic Lethal with Mss4	392659	393912	-1	tRNA-specific 2-thiouridylase	tRNA-specific 2-thiouridylase; responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human homolog TRMU is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF), and can complement yeast null mutant
S000002192	YDL034W	""	""	391783	392127	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps with verified gene GPR1/YDL035C; YDL034W is not an essential gene
S000002193	YDL035C	GPR1	G-Protein coupled Receptor	389172	392057	-1	Plasma membrane G protein coupled receptor (GPCR)	Plasma membrane G protein coupled receptor (GPCR); interacts with the heterotrimeric G protein alpha subunit, Gpa2p, and with Plc1p; sensor that integrates nutritional signals with the modulation of cell fate via PKA and cAMP synthesis
S000002194	YDL036C	PUS9	PseudoUridine Synthase	387513	388901	-1	Mitochondrial tRNA:pseudouridine synthase	Mitochondrial tRNA:pseudouridine synthase; catalyzes the formation of pseudouridine at position 32 in mitochondrial tRNAs; contains an N-terminal mitochondrial targeting sequence; PUS9 has a paralog, RIB2, that arose from the whole genome duplication
S000002195	YDL037C	BSC1	Bypass of Stop Codon	384601	385587	-1	Protein of unconfirmed function	Protein of unconfirmed function; similar to cell surface flocculin Flo11p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; in W303 strain one continuous open reading frame comprising of YDL037C, the intergenic region and YDL039C encodes the gene IMI1
S000002196	YDL038C	""	""	382327	384078	-1	Merged open reading frame	Merged open reading frame; does not encode a discrete protein; YDL038C was originally annotated as an independent ORF, but as a result of a sequence correction, was merged with adjacent ORF PRM7/YDL039C into a single open reading frame
S000002197	YDL039C	PRM7	Pheromone-Regulated Membrane protein	381985	384081	-1	Pheromone-regulated protein	Pheromone-regulated protein; predicted to have one transmembrane segment; promoter contains Gcn4p binding elements; in W303 strain one continuous open reading frame comprising of YDL037C, the intergenic region and YDL039C encodes the IMI1
S000002198	YDL040C	NAT1	N-terminal AcetylTransferase	378874	381438	-1	Subunit of protein N-terminal acetyltransferase NatA	Subunit of protein N-terminal acetyltransferase NatA; NatA comprised of Nat1p, Ard1p, and Nat5p; N-terminally acetylates many proteins to influence multiple processes such as cell cycle progression, heat-shock resistance, mating, sporulation, telomeric silencing and early stages of mitophagy; orthologous to human NAA15; expression of both human NAA10 and NAA15 functionally complements ard1 nat1 double mutant although single mutations are not complemented by their orthologs
S000002199	YDL041W	""	""	378102	378455	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene SIR2/YDL042C
S000002200	YDL042C	SIR2	Silent Information Regulator	376757	378445	-1	Conserved NAD+ dependent histone deacetylase of the Sirtuin family	Conserved NAD+ dependent histone deacetylase of the Sirtuin family; deacetylation targets are primarily nuclear proteins; required for telomere hypercluster formation in quiescent yeast cells; involved in regulation of lifespan; plays roles in silencing at HML, HMR, telomeres, and rDNA; negatively regulates initiation of DNA replication, suppressing early firing of multicopy rDNA origins; functions as regulator of autophagy like mammalian homolog SIRT1, and also of mitophagy
S000002201	YDL043C	PRP11	Pre-mRNA Processing	375680	376480	-1	Subunit of the SF3a splicing factor complex	Subunit of the SF3a splicing factor complex; required for spliceosome assembly
S000002202	YDL044C	MTF2	Mitochondrial Transcription Factor	373967	375289	-1	Mitochondrial protein that interacts with mitochondrial RNA polymerase	Mitochondrial protein that interacts with mitochondrial RNA polymerase; interacts with an N-terminal region of mitochondrial RNA polymerase (Rpo41p) and couples RNA processing and translation to transcription
S000002203	YDL045C	FAD1	FAD synthetase	372688	373608	-1	Flavin adenine dinucleotide (FAD) synthetase	Flavin adenine dinucleotide (FAD) synthetase; performs the second step in synthesis of FAD from riboflavin; mutation is functionally complemented by human FLAD1
S000002204	YDL046W	NPC2	Niemann Pick type C homolog	371240	371761	1	Sterol transport protein, functional homolog of human NPC2 Niemann-Pic	Sterol transport protein, functional homolog of human NPC2 Niemann-Pick disease type C2 protein; together with Ncr1p mediates integration of ergosterol into vacuolar membrane; functions as general “lipid solubilizer”, binds variety of amphiphilic lipid ligands; targeted to vacuole via Vps10p-dependent endosomal vacuolar protein sorting pathway; yeast NPC2 can complement mutations in human NPC2
S000002205	YDL047W	SIT4	Suppressor of Initiation of Transcription	369771	370706	1	Ceramide-activated, type 2A-related serine-threonine phosphatase	Ceramide-activated, type 2A-related serine-threonine phosphatase; functions in G1/S transition of mitotic cycle; controls lifespan, mitochondrial function, cell cycle progression by regulating HXK2 phosphorylation; regulator of COPII coat dephosphorylation; required for ER to Golgi traffic; interacts with Hrr25p kinase; cytoplasmic and nuclear protein that modulates functions mediated by Pkc1p including cell wall and actin cytoskeleton organization; similar to human PP6
S000002206	YDL048C	STP4	protein with similarity to Stp1p	366739	368211	-1	Protein containing a Kruppel-type zinc-finger domain	Protein containing a Kruppel-type zinc-finger domain; similar to Stp1p, Stp2p; predicted transcription factor; relative distribution to the nucleus increases upon DNA replication stress; STP4 has a paralog, STP3, that arose from the whole genome duplication
S000002207	YDL049C	KNH1	Kre9(Nine) Homolog	365068	365874	-1	Protein with similarity to Kre9p	Protein with similarity to Kre9p; Kre9p is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance
S000002208	YDL050C	""	""	364446	364817	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000002209	YDL051W	LHP1	La-Homologous Protein	363952	364779	1	RNA binding protein required for maturation of tRNA and U6 snRNA	RNA binding protein required for maturation of tRNA and U6 snRNA; acts as a molecular chaperone for RNAs transcribed by polymerase III; homologous to human La (SS-B) autoantigen
S000002210	YDL052C	SLC1	SphingoLipid Compensation	362672	363583	-1	1-acyl-sn-glycerol-3-phosphate acyltransferase	1-acyl-sn-glycerol-3-phosphate acyltransferase; catalyzes the acylation of lysophosphatidic acid to form phosphatidic acid, a key intermediate in lipid metabolism; enzymatic activity detected in lipid particles and microsomes
S000002211	YDL053C	PBP4	Pbp1p Binding Protein	361699	362256	-1	Pbp1p binding protein	Pbp1p binding protein; interacts strongly with Pab1p-binding protein 1 (Pbp1p) in the yeast two-hybrid system; also interacts with Lsm12p in a copurification assay; relative distribution to the nucleus increases upon DNA replication stress; also detected in peroxisomes
S000002212	YDL054C	MCH1	MonoCarboxylate transporter Homolog	359825	361285	-1	Protein with similarity to mammalian monocarboxylate permeases	Protein with similarity to mammalian monocarboxylate permeases; monocarboxylate permeases are involved in transport of monocarboxylic acids across the plasma membrane but mutant is not deficient in monocarboxylate transport
S000002213	YDL055C	PSA1	""	355674	356759	-1	GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase)	GDP-mannose pyrophosphorylase (mannose-1-phosphate guanyltransferase); synthesizes GDP-mannose from GTP and mannose-1-phosphate in cell wall biosynthesis; required for normal cell wall structure
S000002214	YDL056W	MBP1	MluI-box Binding Protein	352877	355378	1	Transcription factor	Transcription factor; involved in regulation of cell cycle progression from G1 to S phase, forms a complex with Swi6p that binds to MluI cell cycle box regulatory element in promoters of DNA synthesis genes
S000002215	YDL057W	""	""	351434	352420	1	Putative protein of unknown function	Putative protein of unknown function; YDL057W is not an essential gene
S000002216	YDL058W	USO1	yUSOu - transport in Japanese	345665	351037	1	Essential protein involved in vesicle-mediated ER to Golgi transport	Essential protein involved in vesicle-mediated ER to Golgi transport; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex
S000002217	YDL059C	RAD59	RADiation sensitive	344237	344953	-1	Protein involved DNA double-strand break repair	Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; regulates replication fork progression in DNA ligase I-deficient cells; paralog of Rad52p
S000002218	YDL060W	TSR1	Twenty S rRNA accumulation	341619	343985	1	Protein required for processing of 20S pre-rRNA in the cytoplasm	Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress
S000002219	YDL061C	RPS29B	Ribosomal Protein of the Small subunit	340628	340798	-1	Protein component of the small (40S) ribosomal subunit	Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29B has a paralog, RPS29A, that arose from the whole genome duplication
S000002220	YDL062W	""	""	339857	340282	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YDL063C; YDL062W is not essential
S000002221	YDL063C	SYO1	SYnchronized impOrt or SYmpOrtin	338272	340134	-1	Transport adaptor or symportin	Transport adaptor or symportin; assembly chaperone that co-translationally associates with nascent Rpl5p, preventing aggregation; facilitates synchronized nuclear coimport of two 5S-rRNA binding proteins, Rpl5p and Rpl11p, mediated by import receptor Kap104p; required for biogenesis of the large ribosomal subunit; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
S000002223	YDL065C	PEX19	PEroXisome related	336249	337277	-1	Chaperone and import receptor for newly-synthesized class I PMPs	Chaperone and import receptor for newly-synthesized class I PMPs; binds peroxisomal membrane proteins (PMPs) in the cytoplasm and delivers them to the peroxisome for subsequent insertion into the peroxisomal membrane; interacts with Myo2p and contributes to peroxisome partitioning
S000002224	YDL066W	IDP1	Isocitrate Dehydrogenase, NADP-specific	334835	336121	1	Mitochondrial NADP-specific isocitrate dehydrogenase	Mitochondrial NADP-specific isocitrate dehydrogenase; catalyzes the oxidation of isocitrate to alpha-ketoglutarate; not required for mitochondrial respiration and may function to divert alpha-ketoglutarate to biosynthetic processes
S000002225	YDL067C	COX9	Cytochrome c OXidase	334217	334396	-1	Subunit VIIa of cytochrome c oxidase (Complex IV)	Subunit VIIa of cytochrome c oxidase (Complex IV); Complex IV is the terminal member of the mitochondrial inner membrane electron transport chain
S000002226	YDL068W	""	""	333500	333829	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000002227	YDL069C	CBS1	Cytochrome B Synthesis	333121	333810	-1	Mitochondrial translational activator of the COB mRNA	Mitochondrial translational activator of the COB mRNA; membrane protein that interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader
S000002228	YDL070W	BDF2	BromoDomain Factor	331024	332940	1	Protein involved in transcription initiation	Protein involved in transcription initiation; acts at TATA-containing promoters; associates with the basal transcription factor TFIID; contains two bromodomains; corresponds to the C-terminal region of mammalian TAF1; redundant with Bdf1p; protein abundance increases in response to DNA replication stress; BDF2 has a paralog, BDF1, that arose from the whole genome duplication
S000002229	YDL071C	""	""	330666	331040	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF BDF2/YDL070W
S000002230	YDL072C	YET3	Yeast Endoplasmic reticulum Transmembrane protein	329836	330447	-1	Protein of unknown function	Protein of unknown function; YET3 null mutant decreases the level of secreted invertase; homolog of human BAP31 protein; protein abundance increases in response to DNA replication stress
S000002231	YDL073W	AHK1	Associated with HKr1	326613	329567	1	Scaffold protein in the HKR1 sub-branch of the Hog1p-signaling pathway	Scaffold protein in the HKR1 sub-branch of the Hog1p-signaling pathway; physically interacts with the cytoplasmic domain of Hkr1p, and with Sho1p, Pbs2p, and Ste11p; prevents cross-talk signaling from Hkr1p of the osmotic stress MAPK cascade to the Kss1p MAPK cascade; non-essential gene
S000002232	YDL074C	BRE1	BREfeldin A sensitivity	324047	326149	-1	E3 ubiquitin ligase	E3 ubiquitin ligase; forms heterodimer with Rad6p to regulate K63 polyubiquitination in response to oxidative stress and to monoubiquinate histone H2B-K123, which is required for the subsequent methylation of histone H3-K4 and H3-K79; required for DSBR, transcription, silencing, and checkpoint control; interacts with RNA-binding protein Npl3p, linking histone ubiquitination to mRNA processing; Bre1p-dependent histone ubiquitination promotes pre-mRNA splicing
S000002233	YDL075W	RPL31A	Ribosomal Protein of the Large subunit	322226	322988	1	Ribosomal 60S subunit protein L31A	Ribosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication
S000002234	YDL076C	RXT3	""	320668	321552	-1	Component of the Rpd3L histone deacetylase complex	Component of the Rpd3L histone deacetylase complex; involved in histone deacetylation; protein abundance increases in response to DNA replication stress
S000002235	YDL077C	VAM6	VAcuolar Morphogenesis	316971	320120	-1	Guanine nucleotide exchange factor for the GTPase Gtr1p	Guanine nucleotide exchange factor for the GTPase Gtr1p; subunit of the HOPS endocytic tethering complex; vacuole membrane protein; functions as a Rab GTPase effector, interacting with both GTP- and GDP-bound conformations of Ypt7p; facilitates tethering and promotes membrane fusion events at the late endosome and vacuole; required for both membrane and protein trafficking; component of vacuole-mitochondrion contacts (vCLAMPs) important for lipid transfer between organelles
S000002236	YDL078C	MDH3	Malate DeHydrogenase	315357	316388	-1	Peroxisomal malate dehydrogenase	Peroxisomal malate dehydrogenase; catalyzes interconversion of malate and oxaloacetate; involved in the glyoxylate cycle; mutation in human homolog MDH2 causes early-onset severe encephalopathy
S000002237	YDL079C	MRK1	Mds1p Related Kinase	312951	314748	-1	Glycogen synthase kinase 3 (GSK-3) homolog	Glycogen synthase kinase 3 (GSK-3) homolog; one of four GSK-3 homologs in S. cerevisiae that function to activate Msn2p-dependent transcription of stress responsive genes and that function in protein degradation; MRK1 has a paralog, RIM11, that arose from the whole genome duplication
S000002238	YDL080C	THI3	THIamine metabolism	310642	312471	-1	Regulatory protein that binds Pdc2p and Thi2p transcription factors	Regulatory protein that binds Pdc2p and Thi2p transcription factors; activates thiamine biosynthesis transcription factors Pdc2p and Thi2p by binding to them, but releases and de-activates them upon binding to thiamine pyrophosphate (TPP), the end product of the pathway; has similarity to decarboxylases but enzymatic activity is not detected
S000002239	YDL081C	RPP1A	Ribosomal Protein P1 Alpha	309802	310122	-1	Ribosomal stalk protein P1 alpha	Ribosomal stalk protein P1 alpha; involved in the interaction between translational elongation factors and the ribosome; free (non-ribosomal) P1 stimulates the phosphorylation of the eIF2 alpha subunit (Sui2p) by Gcn2p; accumulation of P1 in the cytoplasm is regulated by phosphorylation and interaction with the P2 stalk component
S000002240	YDL082W	RPL13A	Ribosomal Protein of the Large subunit	308424	309388	1	Ribosomal 60S subunit protein L13A	Ribosomal 60S subunit protein L13A; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13A has a paralog, RPL13B, that arose from the whole genome duplication
S000002241	YDL083C	RPS16B	Ribosomal Protein of the Small subunit	306926	307789	-1	Protein component of the small (40S) ribosomal subunit	Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16B has a paralog, RPS16A, that arose from the whole genome duplication
S000002243	YDL085W	NDE2	NADH Dehydrogenase, External	303211	304848	1	Mitochondrial external NADH dehydrogenase	Mitochondrial external NADH dehydrogenase; catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p are involved in providing the cytosolic NADH to the mitochondrial respiratory chain; NDE2 has a paralog, NDE1, that arose from the whole genome duplication
S000002244	YDL086W	""	""	301413	302234	1	Putative carboxymethylenebutenolidase	Putative carboxymethylenebutenolidase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL086W is not an essential gene
S000002245	YDL087C	LUC7	Lethal Unless Cap-binding complex is produced	300214	300999	-1	Essential protein associated with the U1 snRNP complex	Essential protein associated with the U1 snRNP complex; splicing factor involved in recognition of 5' splice site; contains two zinc finger motifs; N-terminal zinc finger binds pre-mRNA; relocalizes to the cytosol in response to hypoxia
S000002246	YDL088C	ASM4	Anti-Suppressor in Multicopy	298417	300003	-1	FG-nucleoporin component of central core of nuclear pore complex (NPC)	FG-nucleoporin component of central core of nuclear pore complex (NPC); contributes directly to nucleocytoplasmic transport; induces membrane tubulation, which may contribute to nuclear pore assembly; ASM4 has a paralog, NUP53, that arose from the whole genome duplication
S000002247	YDL089W	NUR1	NUclear Rim1	296820	298274	1	Chromosome linkage inner nuclear membrane protein	Chromosome linkage inner nuclear membrane protein; CLIP, involved in perinuclear chromosome tethering and rDNA repeat stability; involved in regulating mitotic exit; dephosphorylated by Cdc14p in anaphase, which promotes timely rDNA segregation and allows mitotic progression; interacts with Csm1p and Lrs4p; null mutant has elevated levels of unequal sister-chromatid exchange; GFP-fusion protein localizes to the nuclear periphery; Cdc28p substrate
S000002248	YDL090C	RAM1	RAS protein and A-factor Maturation	295034	296329	-1	Beta subunit of the CAAX farnesyltransferase (FTase)	Beta subunit of the CAAX farnesyltransferase (FTase); this complex prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit
S000002249	YDL091C	UBX3	UBiquitin regulatory X	293392	294759	-1	Clathrin-coated vesicle component, regulator of endocytosis	Clathrin-coated vesicle component, regulator of endocytosis; copurifies with the DSC ubiquitin ligase complex; UBX (ubiquitin regulatory X) domain-containing protein that interacts with Cdc48p; required for efficient clathrin-mediated endocytosis; ortholog of fission yeast Ucp10
S000002251	YDL093W	PMT5	Protein O-MannosylTransferase	289909	292140	1	Protein O-mannosyltransferase	Protein O-mannosyltransferase; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt3p, can instead interact with Pmt2p in some conditions; target for new antifungals
S000002252	YDL094C	""	""	289572	290081	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verfied gene PMT5/YDL093W; YDL094C is not essential
S000002253	YDL095W	PMT1	Protein O-MannosylTransferase	287059	289512	1	Protein O-mannosyltransferase of the ER membrane	Protein O-mannosyltransferase of the ER membrane; transfers mannose from dolichyl phosphate-D-mannose to protein serine and threonine residues; 1 of 7 related proteins involved in O-glycosylation which is essential for cell wall rigidity; functions as a heterodimer with Pmt2p but can also pair with Pmt3p; involved in ER quality control; amino terminus faces cytoplasm, carboxyl terminus faces ER lumen
S000002254	YDL096C	OPI6	OverProducer of Inositol	286998	287324	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential
S000002255	YDL097C	RPN6	Regulatory Particle Non-ATPase	285391	286695	-1	Essential, non-ATPase regulatory subunit of the 26S proteasome lid	Essential, non-ATPase regulatory subunit of the 26S proteasome lid; required for the assembly and activity of the 26S proteasome; the human homolog (S9 protein) partially rescues Rpn6p depletion; protein abundance increases in response to DNA replication stress
S000002256	YDL098C	SNU23	Small NUclear ribonucleoprotein associated	284581	285165	-1	Component of the U4/U6.U5 snRNP complex	Component of the U4/U6.U5 snRNP complex; involved in mRNA splicing via spliceosome
S000002257	YDL099W	BUG1	Binder of USO1 and GRH1	283419	284444	1	Cis-golgi localized protein involved in ER to Golgi transport	Cis-golgi localized protein involved in ER to Golgi transport; forms a complex with the mammalian GRASP65 homolog, Grh1p; mutants are compromised for the fusion of ER-derived vesicles with Golgi membranes
S000002258	YDL100C	GET3	Guided Entry of Tail-anchored proteins	282112	283176	-1	Guanine nucleotide exchange factor for Gpa1p	Guanine nucleotide exchange factor for Gpa1p; amplifies G-protein signaling; subunit of the GET complex involved in ATP-dependent Golgi-to-ER trafficking; binds as a dimer to the transmembrane domain of tail-anchored proteins in the cytosol, shielding them from aqueous solvent until the Get1p/Get2p heterodimer releases the substrate and inserts it into the ER membrane; acts as a chaperone under ATP-depleted oxidative stress conditions; protein abundance increases under DNA replication stress
S000002259	YDL101C	DUN1	DNA-damage UNinducible	280307	281848	-1	Cell-cycle checkpoint S/T protein kinase	Cell-cycle checkpoint S/T protein kinase; required for transient G2/M arrest after DNA damage, damage-induced transcription, and nuclear-to-cytoplasmic redistribution of Rnr2p-Rnr4p after genotoxic stress and iron deprivation; phosphorylates repair protein Rad55p, transcriptional repressor Sml1p, superoxide dismutase, and ribonucleotide reductase inhibitors Crt1p and Dif1p; functions in the Mec1p pathway to regulate dNTP pools and telomere length; postreplicative repair role
S000002260	YDL102W	POL3	POLymerase	276872	280165	1	Catalytic subunit of DNA polymerase delta	Catalytic subunit of DNA polymerase delta; responsible for most of lagging strand DNA synthesis; required for chromosomal DNA replication during mitosis and meiosis, intragenic recombination, repair of double strand DNA breaks, and DNA replication during nucleotide excision repair (NER)
S000002262	YDL104C	QRI7	""	273653	274876	-1	Protein involved in threonylcarbamoyl adenosine biosynthesis	Protein involved in threonylcarbamoyl adenosine biosynthesis; Sua5p and Qri7p are necessary and sufficient for RNA t6A modification in vitro; highly conserved mitochondrial protein; essential for t6A modification of mitochondrial tRNAs that decode ANN codons; similar to Kae1p and E. coli YgjD, both of which are also required for tRNA t6A modification; when directed to the cytoplasm, complements the essential function of Kae1p in the KEOPS complex
S000002263	YDL105W	NSE4	Non-SMC Element	272389	273597	1	Component of the SMC5-SMC6 complex	Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair
S000002264	YDL106C	PHO2	PHOsphate metabolism	270222	271901	-1	Homeobox transcription factor	Homeobox transcription factor; regulatory targets include genes involved in phosphate metabolism; binds cooperatively with Pho4p to the PHO5 promoter; phosphorylation of Pho2p facilitates interaction with Pho4p; relocalizes to the cytosol in response to hypoxia
S000002265	YDL107W	MSS2	Mitochondrial Splicing	268921	269976	1	Peripherally bound inner membrane protein of the mitochondrial matrix	Peripherally bound inner membrane protein of the mitochondrial matrix; involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p
S000002266	YDL108W	KIN28	protein KINase	267698	268699	1	Ser/Thr protein kinase and subunit of TFIIK, a TFIIH subassembly	Ser/Thr protein kinase and subunit of TFIIK, a TFIIH subassembly; phosphorylates the C-terminal domain (CTD) of RNAPII (Ser5 and Ser7); facilitates recruitment of mRNA 5'-capping and polyadenylation factors to the RNAPII holoenzyme complex; minor role in RNAPII transcription; role in RNAPI transcription; regulates Atg1p kinase complex assembly during nitrogen starvation induced autophagy; associates with cyclin Ccl1p; relocalizes to the cytosol in response to hypoxia; ortholog of human CDK7
S000002267	YDL109C	""	""	265258	267201	-1	Putative lipase	Putative lipase; involved in lipid metabolism; not an essential gene; YDL109C has a paralog, ROG1, that arose from the whole genome duplication
S000002268	YDL110C	TMA17	Translation Machinery Associated	264512	264964	-1	Regulatory gamma subunit of fatty acid synthase (FAS)	Regulatory gamma subunit of fatty acid synthase (FAS); ATPase dedicated chaperone that adapts proteasome assembly to stress; induced upon stress; interacts with Rpt6p to assist its pairing to Rpt3p and early steps in proteasome biogenesis; associates with ribosomes; heterozygous deletion demonstrated increases in chromosome instability in a rad9 deletion background; protein abundance is decreased upon intracellular iron depletion
S000002269	YDL111C	RRP42	Ribosomal RNA Processing	263313	264110	-1	Exosome non-catalytic core component	Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7)
S000002270	YDL112W	TRM3	Transfer RNA Methyltransferase	258915	263225	1	2'-O-ribose methyltransferase	2'-O-ribose methyltransferase; catalyzes the ribose methylation of the guanosine nucleotide at position 18 of tRNAs
S000002271	YDL113C	ATG20	AuTophaGy related	256633	258555	-1	Sorting nexin family member	Sorting nexin family member; required for the cytoplasm-to-vacuole targeting (Cvt) pathway, endosomal sorting and selective autophagy; has a Phox homology domain that binds phosphatidylinositol-3-phosphate; interacts with Snx4p; potential Cdc28p substrate
S000002272	YDL114W	""	""	255604	256530	1	Putative short-chain dehydrogenase/reductase	Putative short-chain dehydrogenase/reductase; YDL114W is not an essential gene
S000002273	YDL115C	IWR1	Interacts With RNA polymerase II	253995	255126	-1	RNA polymerase II transport factor, conserved from yeast to humans	RNA polymerase II transport factor, conserved from yeast to humans; also has a role in transporting RNA polymerase III into the nucleus; interacts with most of the RNAP II subunits; nucleo-cytoplasmic shuttling protein; deletion causes hypersensitivity to K1 killer toxin; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress
S000002274	YDL116W	NUP84	NUclear Pore	251566	253746	1	Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC)	Subunit of the Nup84p subcomplex of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport and NPC biogenesis; also plays roles in several processes that may require localization of genes or chromosomes at the nuclear periphery, including double-strand break repair, transcription and chromatin silencing; homologous to human NUP107
S000002275	YDL117W	CYK3	CYtoKinesis	248581	251238	1	SH3-domain protein located in the bud neck and cytokinetic actin ring	SH3-domain protein located in the bud neck and cytokinetic actin ring; relocalizes from bud neck to nucleus upon DNA replication stress; activates the chitin synthase activity of Chs2p during cytokinesis; suppressor of growth and cytokinesis defects of chs2 phospho-mutants
S000002276	YDL118W	""	""	247302	247682	1	Dubious open reading frame, unlikely to encode a protein	Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein
S000002277	YDL119C	HEM25	HEMe synthesis by SLC25 family member	246689	247612	-1	Mitochondrial glycine transporter	Mitochondrial glycine transporter; required for the transport of glycine into mitochondria for initiation of heme biosynthesis, with YMC1 acting as a secondary transporter; homolog of human SLC25A38, a mitochondrial glycine transporter associated with nonsyndromic autosomal recessive congenital sideroblastic anemia; human SLC25A38 can complement the heme deficiency associated with the null mutant; GFP-fusion protein is induced in response to the DNA-damaging agent MMS
S000002278	YDL120W	YFH1	Yeast Frataxin Homolog	245923	246447	1	Mitochondrial matrix iron chaperone	Mitochondrial matrix iron chaperone; oxidizes and stores iron; interacts with Isu1p to promote Fe-S cluster assembly; mutation results in multiple Fe/S-dependent enzyme deficiencies; human frataxin homolog FXN is mutated in Friedrich's ataxia; human FTL gene can complement yeast yfh1 null mutant
S000002279	YDL121C	EXP1	ER eXport of Pma1	245133	245582	-1	A cargo receptor protein for Pma1p	A cargo receptor protein for Pma1p; works with Psg1p to promote the transport and maturation of Pma1p; localizes to the ER and COPII vesicles; a non-essential protein
S000002280	YDL122W	UBP1	UBiquitin-specific Protease	242552	244981	1	Ubiquitin-specific protease	Ubiquitin-specific protease; removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains
S000002281	YDL123W	SNA4	Sensitivity to NA+	241418	241840	1	Protein of unknown function	Protein of unknown function; localized to the vacuolar outer membrane; predicted to be palmitoylated
S000002282	YDL124W	""	""	240259	241197	1	NADPH-dependent alpha-keto amide reductase	NADPH-dependent alpha-keto amide reductase; reduces aromatic alpha-keto amides, aliphatic alpha-keto esters, and aromatic alpha-keto esters; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress
S000002283	YDL125C	HNT1	Histidine triad NucleoTide-binding	239019	239606	-1	Adenosine 5'-monophosphoramidase	Adenosine 5'-monophosphoramidase; interacts physically and genetically with CDK and TFIIK subunit Kin28p, and genetically with CAK1; member of histidine triad (HIT) superfamily of nucleotide-binding proteins; protein abundance increases in response to DNA replication stress; human homolog HINT1 can complement yeast hnt1 mutant
S000002284	YDL126C	CDC48	Cell Division Cycle	236157	238664	-1	AAA ATPase with protein-unfoldase activity	AAA ATPase with protein-unfoldase activity; subunit of polyUb-selective segregase complex involved in ERAD, INM-associated degradation (INMAD), mitotic spindle disassembly, macroautophagy, PMN, ribosome-associated degradation, ribophagy, homotypic ER membrane fusion, SCF complex disassembly, cell wall integrity, and telomerase regulation; mobilizes membrane-anchored transcription factors by regulated Ub/proteasome-dependent processing (RUP); human ortholog VCP complements a cdc48 mutant
S000002285	YDL127W	PCL2	Pho85 CycLin	234927	235853	1	Cyclin, interacts with cyclin-dependent kinase Pho85p	Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; localizes to sites of polarized cell growth; PCL2 has a paralog, PCL9, that arose from the whole genome duplication
S000002286	YDL128W	VCX1	VaCuolar H+/Ca2+ eXchanger	232652	233887	1	Vacuolar membrane antiporter with Ca2+/H+ and K+/H+ exchange activity	Vacuolar membrane antiporter with Ca2+/H+ and K+/H+ exchange activity; involved in control of cytosolic Ca2+ and K+ concentrations; has similarity to sodium/calcium exchangers, including the bovine Na+/Ca2+,K+ antiporter
S000002287	YDL129W	""	""	231024	231899	1	Protein of unknown function	Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; YDL129W is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress
S000002288	YDL130W	RPP1B	Ribosomal Protein P1 Beta	229906	230527	1	Ribosomal protein P1 beta	Ribosomal protein P1 beta; component of the ribosomal stalk, which is involved in interaction of translational elongation factors with ribosome; free (non-ribosomal) P1 stimulates the phosphorylation of the eIF2 alpha subunit (Sui2p) by Gcn2p; accumulation is regulated by phosphorylation and interaction with the P2 stalk component
S000002289	YDL131W	LYS21	LYSine	227393	228715	1	Homocitrate synthase isozyme	Homocitrate synthase isozyme; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS21 has a paralog, LYS20, that arose from the whole genome duplication
S000002290	YDL132W	CDC53	Cell Division Cycle	224304	226751	1	Cullin	Cullin; structural protein of SCF complexes (which also contain Skp1p, Cdc34p, Hrt1p and an F-box protein) involved in ubiquitination; SCF promotes the G1-S transition by targeting G1 cyclins and the Cln-CDK inhibitor Sic1p for degradation; human homolog CUL1 can complement yeast cdc53 null mutant
S000002291	YDL133W	SRF1	Spo14 Regulatory Factor	222427	223740	1	Regulator of phospholipase D (Spo14p)	Regulator of phospholipase D (Spo14p); interacts with Spo14p and regulates its catalytic activity; capable of buffering the toxicity of C16:0 platelet activating factor, a lipid that accumulates intraneuronally in Alzheimer's patients
S000002292	YDL134C	PPH21	Protein PHosphatase	219662	220771	-1	Catalytic subunit of protein phosphatase 2A, PP2A	Catalytic subunit of protein phosphatase 2A, PP2A; functionally redundant with Pph22p; C-terminally methylated; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; forms nuclear foci upon DNA replication stress; PPH21 has a paralog, PPH22, that arose from the whole genome duplication 
S000002293	YDL133C-A	RPL41B	Ribosomal Protein of the Large subunit	221724	221801	-1	Ribosomal 60S subunit protein L41B	Ribosomal 60S subunit protein L41B; comprises only 25 amino acids; rpl41a rpl41b double null mutant is viable; homologous to mammalian ribosomal protein L41, no bacterial homolog; RPL41B has a paralog, RPL41A, that arose from the whole genome duplication
S000002294	YDL135C	RDI1	Rho GDP Dissociation Inhibitor	218680	219288	-1	Rho GDP dissociation inhibitor	Rho GDP dissociation inhibitor; involved in the localization and regulation of Cdc42p and Rho1p; protein abundance increases in response to DNA replication stress
S000002295	YDL136W	RPL35B	Ribosomal Protein of the Large subunit	217600	218367	1	Ribosomal 60S subunit protein L35B	Ribosomal 60S subunit protein L35B; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35B has a paralog, RPL35A, that arose from the whole genome duplication
S000002296	YDL137W	ARF2	ADP-Ribosylation Factor	216529	217074	1	ADP-ribosylation factor	ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated formation vesicles in intracellular trafficking within the Golgi; ARF2 has a paralog, ARF1, that arose from the whole genome duplication; arf1 arf2 double null mutation is complemented by human ARF1, ARF4, ARF5, or ARF6
S000002297	YDL138W	RGT2	Restores Glucose Transport	213351	215642	1	Plasma membrane high glucose sensor that regulates glucose transport	Plasma membrane high glucose sensor that regulates glucose transport; low affinity sesnor that contains 12 predicted transmembrane segments and a long C-terminal tail required for hexose transporter induction; phosphorylation of the tail by Yck1p/Yck2p facilitates binding to the HXT co-repressors, Mth1p and Std1p; RGT2 has a paralog, SNF3, that arose from the whole genome duplication
S000002298	YDL139C	SCM3	Suppressor of Chromosome Missegregation	211375	212046	-1	Nonhistone component of centromeric chromatin	Nonhistone component of centromeric chromatin; binds to histone H3 variant, Cse4p, and recruits it to centromeres; involved in the assembly and maintenance of Cse4-H4 at centromeres; required for kinetochore assembly and G2/M progression; may protect Cse4p from ubiquitination; homolog of mammalian HJURP
S000002299	YDL140C	RPO21	RNA POlymerase	205360	210561	-1	RNA polymerase II largest subunit B220	RNA polymerase II largest subunit B220; part of central core; phosphorylation of C-terminal heptapeptide repeat domain regulates association with transcription and splicing factors; similar to bacterial beta-prime
S000002301	YDL142C	CRD1	CaRDiolipin synthase	201719	202570	-1	Cardiolipin synthase	Cardiolipin synthase; produces cardiolipin, which is a phospholipid of the mitochondrial inner membrane that is required for normal mitochondrial membrane potential and function and for correct integration of membrane-multispanning proteins into the mitochondrial outer membrane; required to maintain tubular mitochondrial morphology and functions in mitochondrial fusion; also required for normal vacuolar ion homeostasis
S000002302	YDL143W	CCT4	Chaperonin Containing TCP-1	199996	201582	1	Subunit of the cytosolic chaperonin Cct ring complex	Subunit of the cytosolic chaperonin Cct ring complex; related to Tcp1p, required for the assembly of actin and tubulins in vivo
S000002303	YDL144C	""	""	198662	199732	-1	Protein of unknown function	Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YDL144C is not an essential gene; protein abundance increases in response to DNA replication stress
S000002305	YDL146W	LDB17	Low Dye Binding	192750	194225	1	Protein involved in the regulation of endocytosis	Protein involved in the regulation of endocytosis; transiently recruited to actin cortical patches in a SLA1-dependent manner after late coat component assembly; GFP-fusion protein localizes to the periphery, cytoplasm, bud, and bud neck
S000002306	YDL147W	RPN5	Regulatory Particle Non-ATPase	190924	192261	1	Subunit of the CSN and 26S proteasome lid complexes	Subunit of the CSN and 26S proteasome lid complexes; similar to mammalian p55 subunit and to another S. cerevisiae regulatory subunit, Rpn7p; Rpn5p is an essential protein; the COP9 signalosome is also known as the CSN
S000002307	YDL148C	NOP14	NucleOlar Protein	188154	190586	-1	Nucleolar protein	Nucleolar protein; forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA
S000002308	YDL149W	ATG9	AuTophaGy related	184925	187918	1	Lipid scramblase that mediates autophagosomal membrane expansion	Lipid scramblase that mediates autophagosomal membrane expansion; translocates phospholipids between outer and inner leaflets of liposomes; transmembrane protein involved in forming Cvt and autophagic vesicles; cycles between phagophore assembly site (PAS) and other cytosolic punctate structures
S000002309	YDL150W	RPC53	RNA Polymerase C	183343	184611	1	RNA polymerase III subunit C53	RNA polymerase III subunit C53
S000002310	YDL151C	BUD30	BUD site selection	183318	183899	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 96% of ORF overlaps the verified gene RPC53; diploid mutant displays a weak budding pattern phenotype in a systematic assay
S000002311	YDL152W	""	""	182822	183187	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome
S000002312	YDL153C	SAS10	Something About Silencing	181186	183018	-1	Subunit of U3-containing Small Subunit (SSU) processome complex	Subunit of U3-containing Small Subunit (SSU) processome complex; involved in production of 18S rRNA and assembly of small ribosomal subunit; disrupts silencing when overproduced; mutant has increased aneuploidy tolerance; essential gene
S000002313	YDL154W	MSH5	MutS Homolog	178334	181039	1	Protein of the MutS family	Protein of the MutS family; forms a dimer with Msh4p that facilitates crossovers between homologs during meiosis; msh5-Y823H mutation confers tolerance to DNA alkylating agents; homologs present in C. elegans and humans
S000002314	YDL155W	CLB3	CycLin B	176773	178056	1	B-type cyclin involved in cell cycle progression	B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; relative distribution to the nucleus increases upon DNA replication stress; CLB3 has a paralog, CLB4, that arose from the whole genome duplication
S000002315	YDL156W	CMR1	Changed Mutation Rate	174918	176486	1	Nuclear protein with role in transcription, protein quality control	Nuclear protein with role in transcription, protein quality control; localizes to the intranuclear quality control compartment (INQ) in response to proteasome inhibition or DNA replication stress; INQ likely sequesters proteins involved in DNA metabolism for degradation or re-folding; also localizes to coding regions of transcribed genes; contains three WD domains (WD-40 repeat); human ortholog WDR76 also exhibits perinuclear localization under similar stress conditions
S000002316	YDL157C	DMO2	Determines Mitochondrial prOteome	174232	174588	-1	Protein of unknown function	Protein of unknown function; localizes to the ER and peroxisomes; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
S000002318	YDL159W	STE7	STErile	172481	174028	1	Signal transducing MAP kinase kinase	Signal transducing MAP kinase kinase; involved in pheromone response where it phosphorylates Fus3p; involved in the pseudohyphal/invasive growth pathway where it phosphorylates of Kss1p; phosphorylated by Ste11p; degraded by ubiquitin pathway
S000002319	YDL160C	DHH1	DEAD box Helicase Homolog	170410	171930	-1	Cytoplasmic DEAD-box helicase and mRNA decapping activator	Cytoplasmic DEAD-box helicase and mRNA decapping activator; interacts with decapping and deadenylase complexes to coordinate mRNA decapping and decay; regulates general translational repression; translational activator of select mRNAs during filamentous growth, mating and autophagy; cooperates with Ngr1p to promote specific mRNA decay; ATP- and RNA-bound form promotes processing body assembly, while ATPase stimulation by Not1p promotes disassembly; forms cytoplasmic foci on replication stress
S000002320	YDL161W	ENT1	Epsin N-Terminal homology	167714	169078	1	Epsin-like protein involved in endocytosis and actin patch assembly	Epsin-like protein involved in endocytosis and actin patch assembly; K63-specific Ub chain binding protein that functions as an endocytic adaptor; binds clathrin via a clathrin-binding domain motif at C-terminus; contains two ubiquitin-interacting motifs (UIMs); functionally redundant with Ent2p; relocalizes from bud neck to cytoplasm upon DNA replication stress; ENT1 has a paralog, ENT2, that arose from the whole genome duplication
S000002321	YDL162C	""	""	167359	167715	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps ENT1/YDL161W, a verified gene involved in endocytosis and actin cortical patch assembly
S000002322	YDL163W	""	""	166956	167258	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CDC9/YDL164C encoding DNA ligase
S000002323	YDL164C	CDC9	Cell Division Cycle	164987	167254	-1	DNA ligase I found in nucleus and mitochondria	DNA ligase I found in nucleus and mitochondria; essential enzyme that joins Okazaki fragments during DNA replication; also acts in ribonucleotide excision repair, base excision repair, and recombination; DNA ligase I mutants trigger ubiquitination of PCNA at K107, facilitating Rad59p-mediated bypass of unligated Okazaki fragments; human homolog LIG1 can complement yeast cdc9 temperature-sensitive mutant at restrictive temperature
S000002324	YDL165W	CDC36	Cell Division Cycle	164290	164865	1	Component of the CCR4-NOT core complex, involved in mRNA decapping	Component of the CCR4-NOT core complex, involved in mRNA decapping; this complex has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs through deadenylation; basal transcription factor
S000002326	YDL167C	NRP1	N (asparagine)-Rich Protein	160995	163154	-1	Putative RNA binding protein of unknown function	Putative RNA binding protein of unknown function; localizes to stress granules induced by glucose deprivation; predicted to be involved in ribosome biogenesis
S000002327	YDL168W	SFA1	Sensitive to FormAldehyde	159604	160764	1	Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase	Bifunctional alcohol dehydrogenase and formaldehyde dehydrogenase; formaldehyde dehydrogenase activity is glutathione-dependent; functions in formaldehyde detoxification and formation of long chain and complex alcohols, regulated by Hog1p-Sko1p; protein abundance increases in response to DNA replication stress
S000002328	YDL169C	UGX2	Unidentified Gene X	158064	158735	-1	Protein of unknown function	Protein of unknown function; transcript accumulates in response to any combination of stress conditions
S000002329	YDL170W	UGA3	Utilization of GAba	156318	157904	1	Transcriptional activator for GABA-dependent induction of GABA genes	Transcriptional activator for GABA-dependent induction of GABA genes; binds to DNA elements found in the promoters of target genes and increases their expression in the presence of GABA (gamma-aminobutyrate); zinc finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus; examples of GABA genes include UGA1, UGA2, and UGA4
S000002330	YDL171C	GLT1	GLuTamate synthase	149203	155640	-1	NAD(+)-dependent glutamate synthase (GOGAT)	NAD(+)-dependent glutamate synthase (GOGAT); synthesizes glutamate from glutamine and alpha-ketoglutarate; with Gln1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source; assembles into filaments as cells approach stationary phase and under cytosolic acidification and starvation conditions
S000002331	YDL172C	""	""	148607	149086	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000002332	YDL173W	PAR32	Phosphorylated After Rapamycin	148191	149078	1	Low complexity protein	Low complexity protein; mediates inhibition of Mep1p and Mep3p activity; hyperphosphorylated upon rapamycin treatment in a Tap42p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; phosphorylation and localization regulated by TORC1-effector kinase, Npr1p
S000002333	YDL174C	DLD1	D-Lactate Dehydrogenase	145826	147589	-1	Major mitochondrial D-lactate dehydrogenase	Major mitochondrial D-lactate dehydrogenase; oxidizes D-lactate to pyruvate, transcription is heme-dependent, repressed by glucose, and derepressed in ethanol or lactate; located in the mitochondrial inner membrane
S000002334	YDL175C	AIR2	Arginine methyltransferase-Interacting RING finger protein	144484	145518	-1	RNA-binding subunit of the TRAMP nuclear RNA surveillance complex	RNA-binding subunit of the TRAMP nuclear RNA surveillance complex; involved in nuclear RNA processing and degradation; involved in TRAMP complex assembly as a bridge between Mtr4p and Trf4p; stimulates the poly(A) polymerase activity of Pap2p in vitro; has 5 zinc knuckle motifs; AIR2 has a paralog, AIR1, that arose from the whole genome duplication; Air2p and Air1p have nonredundant roles in regulation of substrate specificity of the exosome
S000002335	YDL176W	GID12	Glucose Induced Degradation deficient	142097	144223	1	Protein of unknown function involved in regulating assembly of Gid4p i	Protein of unknown function involved in regulating assembly of Gid4p into the GID E3 ligase complex; interacts with GID complex components; involved in actin patch formation; computationally predicted to be involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; non-essential gene
S000002336	YDL177C	""	""	141209	141721	-1	Putative protein of unknown function	Putative protein of unknown function; similar to the mouse IMPACT gene; YDL177C is not an essential gene
S000002337	YDL178W	DLD2	D-Lactate Dehydrogenase	139522	141114	1	D-2-hydroxyglutarate dehydrogenase, and minor D-lactate dehydrogenase	D-2-hydroxyglutarate dehydrogenase, and minor D-lactate dehydrogenase; mitochondrial matrix protein that oxidizes D-2-hydroxyglutarate (D-2HG), an oncometabolite, to alpha-ketoglutarate with a minor role in lactate catabolism; located in the mitochondrial matrix
S000002338	YDL179W	PCL9	Pho85 CycLin	138291	139205	1	Cyclin	Cyclin; forms a functional kinase complex with Pho85p cyclin-dependent kinase (Cdk), expressed in late M/early G1 phase, activated by Swi5p; PCL9 has a paralog, PCL2, that arose from the whole genome duplication
S000002339	YDL180W	AIT1	Amino acid dependent Inhibitor of Torc1	135896	137539	1	G protein-coupled receptor-like protein	G protein-coupled receptor-like protein; involved in negatively regulating TORC1 activity; green fluorescent protein (GFP)-fusion protein localizes to the vacuole
S000002340	YDL181W	INH1	INHibitor (of F1F0-ATPase)	135179	135436	1	Protein that inhibits ATP hydrolysis by the F1F0-ATP synthase	Protein that inhibits ATP hydrolysis by the F1F0-ATP synthase; inhibitory function is enhanced by stabilizing proteins Stf1p and Stf2p; has a calmodulin-binding motif and binds calmodulin in vitro; INH1 has a paralog, STF1, that arose from the whole genome duplication
S000002341	YDL182W	LYS20	LYSine requiring	133437	134723	1	Homocitrate synthase isozyme and functions in DNA repair	Homocitrate synthase isozyme and functions in DNA repair; catalyzes the condensation of acetyl-CoA and alpha-ketoglutarate to form homocitrate, which is the first step in the lysine biosynthesis pathway; LYS20 has a paralog, LYS21, that arose from the whole genome duplication
S000002342	YDL183C	MRX19	Mitochondrial oRganization of gene eXpression (MIOREX)	130871	131833	-1	Protein that may form an active mitochondrial KHE system	Protein that may form an active mitochondrial KHE system; mitochondrial inner-membrane protein; non-essential gene; KHE system stands for K+/H+ exchanger system
S000002343	YDL184C	RPL41A	Ribosomal Protein of the Large subunit	130407	130484	-1	Ribosomal 60S subunit protein L41A	Ribosomal 60S subunit protein L41A; comprises only 25 amino acids; rpl41a rpl41b double null mutant is viable; homologous to mammalian ribosomal protein L41, no bacterial homolog; RPL41A has a paralog, RPL41B, that arose from the whole genome duplication
S000002344	YDL185W	VMA1	Vacuolar Membrane Atpase	126787	130002	1	Subunit A of the V1 peripheral membrane domain of V-ATPase	Subunit A of the V1 peripheral membrane domain of V-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease; the V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase) has eight subunits; involved in methionine restriction extension of chronological lifespan in an autophagy-dependent manner
S000002345	YDL186W	""	""	125616	126449	1	Putative protein of unknown function	Putative protein of unknown function; YDL186W is not an essential gene
S000002346	YDL187C	""	""	125509	125838	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000002347	YDL188C	PPH22	Protein PHosphatase	123865	124998	-1	Catalytic subunit of protein phosphatase 2A, PP2A	Catalytic subunit of protein phosphatase 2A, PP2A; functionally redundant with Pph21p; C-terminally methylated; forms alternate complexes with several regulatory subunits; involved in signal transduction and regulation of mitosis; protein abundance increases in response to DNA replication stress; dephosphorylates Tel1p/Mec1p-phosphorylated Cdc13p to promote telomerase release from telomeres at G2/M; PPH22 has a paralog, PPH21, that arose from the whole genome duplication
S000002348	YDL189W	RBS1	RNA-Binding Suppressor of PAS kinase	122216	123589	1	Protein involved in assembly of the RNA Pol III complex	Protein involved in assembly of the RNA Pol III complex; high copy suppressor of Pol III assembly mutation and psk1 psk2 mutations that confer temperature-sensitivity for galactose utilization; physically interacts with Pol III; proposed to bind single-stranded nucleic acids via its R3H domain; capable of forming the prion [VTS1+]
S000002349	YDL190C	UFD2	Ubiquitin Fusion Degradation	118707	121592	-1	Conserved ubiquitin (Ub) chain assembly factor (E4)	Conserved ubiquitin (Ub) chain assembly factor (E4); forms branched Ub chains on substrates destined for degradation, catalyzing K48-linked multi-monoubiquitination of K29-linked Ub chains assembled by Ub-ligase Ufd4p; involved in Ub-fusion degradation pathway, OLE membrane fluidity pathway, stress response survival, and ERAD pathway for misfolded proteins where it promotes ubiquitination and transfer of ERAD substrates to Rad23p; U-box family member; homologous to human Ufd2a, Ufd2b
S000002350	YDL191W	RPL35A	Ribosomal Protein of the Large subunit	117664	118517	1	Ribosomal 60S subunit protein L35A	Ribosomal 60S subunit protein L35A; homologous to mammalian ribosomal protein L35 and bacterial L29; RPL35A has a paralog, RPL35B, that arose from the whole genome duplication
S000002351	YDL192W	ARF1	ADP-Ribosylation Factor	116321	116866	1	ADP-ribosylation factor	ADP-ribosylation factor; GTPase of the Ras superfamily that regulates coated vesicle formation during intracellular trafficking within the Golgi; regulates mitochondrial fission, fusion and function, controlling the flow of fatty acids and metabolites from lipid droplets to peroxisomes and mitochondria; localizes to the Golgi apparatus and the active form to ER and lipid droplets; arf1 arf2 double null mutation is complemented by human ARF1, ARF4, ARF5, or ARF6
S000002352	YDL193W	NUS1	Nuclear Undecaprenyl pyrophosphate Synthase	114672	115799	1	Forms dehydrodolichyl diphosphate synthase complex with RER2 or SRT1	Forms dehydrodolichyl diphosphate synthase complex with RER2 or SRT1; tet-repressible mutant shows accumulation of hypoglycosylated forms of CPY, suggesting that Nus1p may be involved in protein trafficking; mutations in human homolog NUS1 have been implicated in congenital scoliosis, neurological impairment, refractory epilepsy, hearing deficit, and visual impairment; human cis-prenyltransferase complex complements yeast null mutant
S000002353	YDL194W	SNF3	Sucrose NonFermenting	111580	114234	1	Plasma membrane low glucose sensor, regulates glucose transport	Plasma membrane low glucose sensor, regulates glucose transport; high affinity sensor that contains 12 predicted transmembrane segments and a long C-terminal tail required for induction of hexose transporters; also senses fructose and mannose; SNF3 has a paralog, RGT2, that arose from the whole genome duplication
S000002354	YDL195W	SEC31	SECretory	107208	111029	1	Component of the Sec13p-Sec31p complex of the COPII vesicle coat	Component of the Sec13p-Sec31p complex of the COPII vesicle coat; COPII coat is required for vesicle formation in ER to Golgi transport; mutant has increased aneuploidy tolerance
S000002356	YDL197C	ASF2	Anti-Silencing Function	104917	106494	-1	Anti-silencing protein	Anti-silencing protein; causes derepression of silent loci when overexpressed
S000002357	YDL198C	GGC1	GDP/GTP Carrier	103649	104551	-1	Mitochondrial GTP/GDP transporter	Mitochondrial GTP/GDP transporter; essential for mitochondrial genome maintenance; has a role in mitochondrial iron transport; member of the mitochondrial carrier family
S000002358	YDL199C	""	""	101290	103353	-1	Putative transporter	Putative transporter; member of the sugar porter family
S000002359	YDL200C	MGT1	O-6-MethylGuanine-DNA methylTransferase	100501	101067	-1	DNA repair methyltransferase (6-O-methylguanine-DNA methylase)	DNA repair methyltransferase (6-O-methylguanine-DNA methylase); involved in protection against DNA alkylation damage; localizes to the peroxisome in a Pex5p-dependent manner
S000002360	YDL201W	TRM8	Transfer RNA Methyltransferase	99561	100421	1	Catalytic subunit of a tRNA methyltransferase complex	Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p
S000002361	YDL202W	MRPL11	Mitochondrial Ribosomal Protein, Large subunit	98475	99224	1	Mitochondrial ribosomal protein of the large subunit	Mitochondrial ribosomal protein of the large subunit; localizes to vacuole in response to H2O2
S000002362	YDL203C	ACK1	Activator of C Kinase	96082	97953	-1	Protein that functions in the cell wall integrity pathway	Protein that functions in the cell wall integrity pathway; functions upstream of Pkc1p; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria
S000002363	YDL204W	RTN2	ReTiculoN-like	94605	95786	1	Reticulon protein	Reticulon protein; involved in nuclear pore assembly and maintenance of tubular ER morphology; promotes membrane curvature; regulates the ER asymmetry-induced inheritance block during ER stress; role in ER-derived peroxisomal biogenesis; interacts with Sec6p, Yip3p, and Sbh1p; less abundant than RTN1; member of RTNLA (reticulon-like A) subfamily; protein increases in abundance and relocalizes to plasma membrane upon DNA replication stress
S000002364	YDL205C	HEM3	HEMe biosynthesis	92762	93745	-1	Porphobilinogen deaminase	Porphobilinogen deaminase; catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme; human homolog HMBS can complement yeast mutant and allow growth of haploid null after sporulation of a heterozygous diploid
S000002365	YDL206W	YCX1	Yeast CCX	90176	92464	1	Protein involved in calcium-induced activation of calcineurin	Protein involved in calcium-induced activation of calcineurin; homologous to proteins in Candida and Cryptococcus; YCX1 is not an essential protein
S000002366	YDL207W	GLE1	GLFG (glycine-leucine-phenylalanine-glycine) LEthal	88248	89864	1	Cytoplasmic nucleoporin required for polyadenylated mRNA export	Cytoplasmic nucleoporin required for polyadenylated mRNA export; contains a nuclear export signal; when bound to inositol hexakisphosphate (IP6), functions as an activator for the Dbp5p ATPase activity at the nuclear pore complex during mRNA export; mediates translation initiation; required for efficient translation termination
S000002367	YDL208W	NHP2	Non-Histone Protein	87512	87982	1	Protein related to mammalian high mobility group (HMG) proteins	Protein related to mammalian high mobility group (HMG) proteins; nuclear protein; essential for function of H/ACA-type snoRNPs, which are involved in 18S rRNA processing
S000002368	YDL209C	CWC2	Complexed With Cef1p	86207	87226	-1	Member of the NineTeen Complex (NTC)	Member of the NineTeen Complex (NTC); this complex contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2
S000002369	YDL210W	UGA4	Utilization of GAba	84270	85985	1	GABA (gamma-aminobutyrate) permease	GABA (gamma-aminobutyrate) permease; serves as a GABA transport protein involved in the utilization of GABA as a nitrogen source; catalyzes the transport of putrescine and delta-aminolevulinic acid (ALA); localized to the vacuolar membrane
S000002370	YDL211C	""	""	79294	80412	-1	Protein of unknown function	Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YDL211C has a paralog, TDA7, that arose from the whole genome duplication
S000002371	YDL212W	SHR3	Super high Histidine Resistant	78426	79058	1	Endoplasmic reticulum packaging chaperone	Endoplasmic reticulum packaging chaperone; required for incorporation of amino acid permeases into COPII coated vesicles for transport to the cell surface
S000002372	YDL213C	NOP6	NucleOlar Protein	77289	77966	-1	rRNA-binding protein required for 40S ribosomal subunit biogenesis	rRNA-binding protein required for 40S ribosomal subunit biogenesis; contains an RNA recognition motif (RRM); hydrophilin essential to overcome the stress of the desiccation-rehydration process; NOP6 may be a fungal-specific gene as no homologs have been yet identified in higher eukaryotes
S000002373	YDL214C	PRR2	Pheromone Response Regulator	74446	76545	-1	Serine/threonine protein kinase	Serine/threonine protein kinase; inhibits pheromone induced signalling downstream of MAPK, possibly at the level of the Ste12p transcription factor; mutant has increased aneuploidy tolerance; PRR2 has a paralog, NPR1, that arose from the whole genome duplication
S000002374	YDL215C	GDH2	Glutamate DeHydrogenase	70640	73918	-1	NAD(+)-dependent glutamate dehydrogenase	NAD(+)-dependent glutamate dehydrogenase; degrades glutamate to ammonia and alpha-ketoglutarate; expression sensitive to nitrogen catabolite repression and intracellular ammonia levels; genetically interacts with GDH3 by suppressing stress-induced apoptosis
S000002375	YDL216C	RRI1	Regulator of Rub1 specific Isopeptidase	68997	70319	-1	Catalytic subunit of the COP9 signalosome (CSN) complex	Catalytic subunit of the COP9 signalosome (CSN) complex; acts as an isopeptidase in cleaving the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; metalloendopeptidase involved in the adaptation to pheromone signaling; involved in modulation of genes controlling amino acid and lipid metabolism, and ergosterol biosynthesis
S000002376	YDL217C	TIM22	Translocase of the Inner Mitochondrial membrane	67983	68606	-1	Essential core component of mitochondrial TIM22 complex	Essential core component of mitochondrial TIM22 complex; involved in insertion of polytopic proteins into inner membrane; forms channel through which proteins are imported; different regions of Tim22p have functionally diverse roles in recruiting partner subunits to core channel
S000002377	YDL218W	""	""	66493	67446	1	Putative protein of unknown function	Putative protein of unknown function; YDL218W transcription is regulated by Azf1p and induced by starvation and aerobic conditions; expression also induced in cells treated with the mycotoxin patulin
S000002378	YDL219W	DTD1	D-Tyr-tRNA(Tyr) Deacylase	65242	65765	1	D-Tyr-tRNA(Tyr) deacylase	D-Tyr-tRNA(Tyr) deacylase; functions in protein translation, may affect nonsense suppression via alteration of the protein synthesis machinery; ubiquitous among eukaryotes
S000002379	YDL220C	CDC13	Cell Division Cycle	62244	65018	-1	Single stranded DNA-binding protein found at TG1-3 telomere G-tails	Single stranded DNA-binding protein found at TG1-3 telomere G-tails; key roles in regulation of telomerase, telomere end protection, conventional telomere replication; regulates telomere replication through recruitment of specific sub-complexes, essential function is telomere capping; forms homodimer via N-terminus; disruption of dimerization leads to short telomeres; autophagy and proteasome are involved in Cdc13p degradation; differentially phosphorylated through cell cycle
S000002381	YDL222C	FMP45	Found in Mitochondrial Proteome	60872	61801	-1	Integral membrane protein localized to mitochondria	Integral membrane protein localized to mitochondria; required for sporulation and maintaining sphingolipid content; similar to SUR7; FMP45 has a paralog, YNL194C, that arose from the whole genome duplication
S000002382	YDL223C	HBT1	HuB1 Target	57265	60405	-1	Shmoo tip protein, substrate of Hub1p ubiquitin-like protein	Shmoo tip protein, substrate of Hub1p ubiquitin-like protein; mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis; HBT1 has a paralog, YNL195C, that arose from the whole genome duplication
S000002383	YDL224C	WHI4	WHIskey	54397	56346	-1	Putative RNA binding protein	Putative RNA binding protein; regulates the cell size requirement for passage through Start and commitment to cell division; WHI4 has a paralog, WHI3, that arose from the whole genome duplication
S000002384	YDL225W	SHS1	Seventh Homolog of Septin	52445	54100	1	Component of the septin ring that is required for cytokinesis	Component of the septin ring that is required for cytokinesis; present at the ends of rod-like septin hetero-oligomers; C-terminal extension is important for recruitment of Bni5p to the mother-bud neck, which in turn is required for Myo1p recruitment and cytokinesis; undergoes sumoylation and phosphorylation during mitosis; protein abundance increases in response to DNA replication stress
S000002385	YDL226C	GCS1	Growth Cold Sensitive	51115	52173	-1	ADP-ribosylation factor GTPase activating protein (ARF GAP)	ADP-ribosylation factor GTPase activating protein (ARF GAP); involved in ER-Golgi transport; required for prospore membrane formation; regulates phospholipase Spo14p; shares functional similarity with Glo3p; GCS1 has a paralog, SPS18, that arose from the whole genome duplication
S000002386	YDL227C	HO	HOmothallic switching endonuclease	46271	48031	-1	Site-specific endonuclease	Site-specific endonuclease; required for gene conversion at the MAT locus (homothallic switching) through the generation of a ds DNA break; expression restricted to mother cells in late G1 as controlled by Swi4p-Swi6p, Swi5p, and Ash1p
S000002388	YDL229W	SSB1	Stress-Seventy subfamily B	44065	45906	1	Cytoplasmic ATPase that is a ribosome-associated molecular chaperone	Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; functions with J-protein partner Zuo1p; may be involved in folding of newly-made polypeptide chains; member of the HSP70 family; interacts with phosphatase subunit Reg1p; SSB1 has a paralog, SSB2, that arose from the whole genome duplication
S000002389	YDL230W	PTP1	Protein Tyrosine Phosphatase	42700	43707	1	Phosphotyrosine-specific protein phosphatase	Phosphotyrosine-specific protein phosphatase; dephosphorylates a broad range of substrates in vivo, including Fpr3p; localized to the cytoplasm and the mitochondria; proposed to be a negative regulator of filamentation
S000002390	YDL231C	BRE4	BREfeldin A sensitivity	38867	42244	-1	Zinc finger protein containing five transmembrane domains	Zinc finger protein containing five transmembrane domains; null mutant exhibits strongly fragmented vacuoles and sensitivity to brefeldin A, a drug which is known to affect intracellular transport
S000002391	YDL232W	OST4	OligoSaccharylTransferase	38487	38597	1	Subunit of the oligosaccharyltransferase complex of the ER lumen	Subunit of the oligosaccharyltransferase complex of the ER lumen; complex catalyzes protein asparagine-linked glycosylation; type I membrane protein required for incorporation of Ost3p or Ost6p into the OST complex
S000002392	YDL233W	MFG1	Morphogenetic regulator of Filamentous Growth	36797	38173	1	Regulator of filamentous growth	Regulator of filamentous growth; interacts with FLO11 promoter and regulates FLO11 expression; binds to transcription factors Flo8p and Mss11p; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YDL233W is not an essential gene
S000002393	YDL234C	GYP7	Gtpase-activating protein for Ypt7 Protein	34237	36477	-1	GTPase-activating protein for yeast Rab family members	GTPase-activating protein for yeast Rab family members; members include Ypt7p (most effective), Ypt1p, Ypt31p, and Ypt32p (in vitro); involved in vesicle mediated protein trafficking; contains a PH-like domain
S000002394	YDL235C	YPD1	tYrosine Phosphatase Dependent	33415	33918	-1	Osmotic stress-responsive phosphorelay intermediate sensor protein	Osmotic stress-responsive phosphorelay intermediate sensor protein; phosphorylated by the plasma membrane sensor Sln1p in response to osmotic stress and then in turn phosphorylates the response regulators Ssk1p in the cytosol and Skn7p in the nucleus
S000002395	YDL236W	PHO13	PHOsphate metabolism	32296	33234	1	Conserved phosphatase acting as a metabolite repair enzyme	Conserved phosphatase acting as a metabolite repair enzyme; shows specific dephosphorylating activity on two side-products of central carbohydrate metabolism, 2-phosphoglycolate and 4-phosphoerythronate; alkaline phosphatase specific for p-nitrophenyl phosphate; also has protein phosphatase activity; human ortholog PGP shows similar substrate specificity, deletion causes similar metabolite accumulation phenotypes, suggesting conserved role in eliminating glycolytic byproducts
S000002396	YDL237W	AIM6	Altered Inheritance rate of Mitochondria	30657	31829	1	Protein of unknown function	Protein of unknown function; required for respiratory growth; YDL237W is not an essential gene
S000002397	YDL238C	GUD1	GUanine Deaminase	28985	30454	-1	Guanine deaminase	Guanine deaminase; a catabolic enzyme of the guanine salvage pathway producing xanthine and ammonia from guanine; activity is low in exponentially-growing cultures but expression is increased in post-diauxic and stationary-phase cultures
S000002398	YDL239C	ADY3	Accumulation of DYads	26403	28775	-1	Protein required for spore wall formation	Protein required for spore wall formation; subunit of leading edge protein (LEP) complex (Ssp1-Ady3-Don1-Irc10) that forms ring-like structure at leading edge of prospore membrane during meiosis II; mediates assembly of LEP complex, formation of ring-like structure via interaction with spindle pole body components, and prospore membrane maturation; also detected in peroxisomes; potentially phosphorylated by Cdc28p; ADY3 has a paralog, CNM67, that arose from the whole genome duplication
S000002399	YDL240W	LRG1	Lim-RhoGap homolog	22823	25876	1	GTPase-activating protein (GAP)	GTPase-activating protein (GAP); contains Rho1p-specific GAP activity, interacting with activated forms of Rho1p; functions along with Sac7p as a negative regulator of the Pkc1p-mediated cell wall integrity signaling pathway; negative regulator of cell wall 1,3-beta-glucan biosynthesis; required for efficient cell fusion; contains a RhoGAP domain and three Lin-11-Isl1-Mec-3 (LIM) domains
S000002400	YDL241W	""	""	20635	21006	1	Putative protein of unknown function	Putative protein of unknown function; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum; YDL241W is not an essential gene
S000002401	YDL242W	""	""	18959	19312	1	Putative protein of unknown function	Putative protein of unknown function; conserved across S. cerevisiae strains
S000002402	YDL243C	AAD4	Aryl-Alcohol Dehydrogenase	17577	18566	-1	Putative aryl-alcohol dehydrogenase	Putative aryl-alcohol dehydrogenase; involved in oxidative stress response; similar to P. chrysosporium aryl-alcohol dehydrogenase; expression induced in cells treated with the mycotoxin patulin; members of the AAD gene family comprise three pairs (AAD3 + AAD15, AAD6/AAD16 + AAD4, AAD10 + AAD14) whose two genes are more related to one another than to other members of the family
S000002408	YDR001C	NTH1	Neutral TreHalase	450220	452475	-1	Neutral trehalase, degrades trehalose	Neutral trehalase, degrades trehalose; required for thermotolerance and may mediate resistance to other cellular stresses; phosphorylated and activated by Cdc28p at the G1/S phase transition to coordinately regulate carbohydrate metabolism and the cell cycle; inhibited by Dcs1p; NTH1 has a paralog, NTH2, that arose from the whole genome duplication
S000002409	YDR002W	YRB1	Yeast Ran Binder	453045	453650	1	Ran GTPase binding protein	Ran GTPase binding protein; involved in nuclear protein import and RNA export, ubiquitin-mediated protein degradation during the cell cycle; shuttles between the nucleus and cytoplasm; is essential; homolog of human RanBP1
S000002410	YDR003W	RCR2	Resistance to Congo Red	454122	454754	1	Vacuolar ubiquitin ligase-substrate adaptor	Vacuolar ubiquitin ligase-substrate adaptor; delivered to vacuole using ubiquitin as a sorting signal; presumably functions within the endosomal-vacuolar trafficking pathway, affecting events that determine whether plasma membrane proteins are degraded or routed to the plasma membrane; RCR2 has a paralog, RCR1, that arose from the whole genome duplication
S000002411	YDR004W	RAD57	RADiation sensitive	455201	456583	1	Protein that stimulates strand exchange	Protein that stimulates strand exchange; stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p
S000002412	YDR005C	MAF1	""	456836	458103	-1	Highly conserved negative regulator of RNA polymerase III	Highly conserved negative regulator of RNA polymerase III; involved in tRNA processing and stability; inhibits tRNA degradation via rapid tRNA decay (RTD) pathway; binds N-terminal domain of Rpc160p subunit of Pol III to prevent closed-complex formation; regulated by phosphorylation mediated by TORC1, protein kinase A, Sch9p, casein kinase 2; localizes to cytoplasm during vegetative growth and translocates to nucleus and nucleolus under stress conditions
S000002413	YDR006C	SOK1	Suppressor Of Kinase	458542	461247	-1	Protein of unknown function	Protein of unknown function; overexpression suppresses the growth defect of mutants lacking protein kinase A activity; involved in cAMP-mediated signaling; localized to the nucleus; similar to the mouse testis-specific protein PBS13
S000002414	YDR007W	TRP1	TRyPtophan	461842	462516	1	Phosphoribosylanthranilate isomerase	Phosphoribosylanthranilate isomerase; catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA); enhances vegetative growth at low and high temperatures when used as an auxotrophic marker in strains such as W303
S000002415	YDR008C	""	""	462252	462602	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps TRP1/YDR007W on opposite strand
S000002416	YDR009W	GAL3	GALactose metabolism	463434	464996	1	Transcriptional regulator	Transcriptional regulator; involved in activation of the GAL genes in response to galactose; forms a complex with Gal80p to relieve Gal80p inhibition of Gal4p; binds galactose and ATP but does not have galactokinase activity; GAL3 has a paralog, GAL1, that arose from the whole genome duplication
S000002417	YDR010C	""	""	465051	465383	-1	Putative protein of unknown function	Putative protein of unknown function; conserved across S. cerevisiae strains
S000002418	YDR011W	SNQ2	Sensitivity to 4-NitroQuinoline-N-oxide	465919	470424	1	Plasma membrane ATP-binding cassette (ABC) transporter	Plasma membrane ATP-binding cassette (ABC) transporter; multidrug transporter involved in multidrug resistance and resistance to singlet oxygen species
S000002420	YDR013W	PSF1	Partner of Sld Five	473157	473783	1	Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p)	Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p); complex is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
S000002421	YDR014W	RAD61	RADiation sensitive	474046	475989	1	Subunit of a complex that inhibits sister chromatid cohesion	Subunit of a complex that inhibits sister chromatid cohesion; also negatively regulates chromosome condensation; inhibited by Eco1p-acetylated cohesin subunits Smc3p and Mcd1p; binds Smc3p ATPase head of cohesin; related to the human Wapl protein that controls the association of cohesin with chromatin
S000002422	YDR015C	""	""	477960	478199	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene HED1/YDR014W-A
S000002424	YDR017C	KCS1	pKC1 Suppressor	479115	482267	-1	Inositol hexakisphosphate and inositol heptakisphosphate kinase	Inositol hexakisphosphate and inositol heptakisphosphate kinase; generation of high energy inositol pyrophosphates by Kcs1p is required for many processes such as vacuolar biogenesis, stress response, RNA polymerase I-mediated rRNA transcription and telomere maintenance; inositol hexakisphosphate is also known as IP6; inositol heptakisphosphate is also known as IP7
S000002425	YDR018C	""	""	482670	483860	-1	Probable membrane protein with three predicted transmembrane domains	Probable membrane protein with three predicted transmembrane domains; similar to C. elegans F55A11.5 and maize 1-acyl-glycerol-3-phosphate acyltransferase; YDR018C has a paralog, CST26, that arose from the whole genome duplication
S000002426	YDR019C	GCV1	GlyCine cleaVage	484163	485365	-1	T subunit of the mitochondrial glycine decarboxylase complex	T subunit of the mitochondrial glycine decarboxylase complex; glycine decarboxylase is required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of levels of 5,10-methylene-THF in the cytoplasm
S000002427	YDR020C	DAS2	Dst1-delta 6-Azauracil Sensitivity	485746	486444	-1	Putative protein of unknown function	Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; weak similarity with uridine kinases and with phosphoribokinases
S000002428	YDR021W	FAL1	eukaryotic translation initiation factor Four A Like	486804	488003	1	Nucleolar protein required for maturation of 18S rRNA	Nucleolar protein required for maturation of 18S rRNA; member of the eIF4A subfamily of DEAD-box ATP-dependent RNA helicases; 18S rRNA biogenesis defect of the null mutant is functionally complemented by human EIF4A3
S000002429	YDR022C	ATG31	AuTophaGy related	488072	488662	-1	Autophagy-specific protein required for autophagosome formation	Autophagy-specific protein required for autophagosome formation; forms a complex with Atg17p and Atg29p that localizes other proteins to the pre-autophagosomal structure; constitutively phosphorylated, and phosphorylation of residue S174 is required for function; high-copy suppressor of CIK1 deletion
S000002431	YDR024W	FYV1	Function required for Yeast Viability	491017	491502	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; mutation decreases survival upon exposure to K1 killer toxin
S000002432	YDR025W	RPS11A	Ribosomal Protein of the Small subunit	491515	492324	1	Protein component of the small (40S) ribosomal subunit	Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S11 and bacterial S17; N-terminally propionylated in vivo; RPS11A has a paralog, RPS11B, that arose from the whole genome duplication
S000002433	YDR026C	NSI1	NTS1 SIlencing protein	492556	494268	-1	RNA polymerase I termination factor	RNA polymerase I termination factor; binds to rDNA terminator element, required for efficient Pol I termination; required for rDNA silencing at NTS1; facilities association of Sir2p with NTS1, contributes to rDNA stability and cell longevity; interacts physically with Fob1p and RENT subunits, Sir2p and Net1p; may interact with ribosomes, based on co-purification experiments; Myb-like DNA-binding protein; NSI1 has a paralog, REB1, that arose from the whole genome duplication
S000002434	YDR027C	VPS54	Vacuolar Protein Sorting	494649	497318	-1	Component of the GARP (Golgi-associated retrograde protein) complex	Component of the GARP (Golgi-associated retrograde protein) complex; GARP is required for the recycling of proteins from endosomes to the late Golgi, and for mitosis after DNA damage induced checkpoint arrest; potentially phosphorylated by Cdc28p; members of the GARP complex are Vps51p-Vps52p-Vps53p-Vps54p
S000002435	YDR028C	REG1	REsistance to Glucose repression	497835	500879	-1	Regulatory subunit of type 1 protein phosphatase Glc7p	Regulatory subunit of type 1 protein phosphatase Glc7p; involved in negative regulation of glucose-repressible genes; involved in regulation of the nucleocytoplasmic shuttling of Hxk2p; REG1 has a paralog, REG2, that arose from the whole genome duplication
S000002436	YDR029W	""	""	501100	501414	1	Putative protein of unknown function	Putative protein of unknown function; conserved among S. cerevisiae strains; YDR029W is not an essential gene
S000002437	YDR030C	RAD28	RADiation sensitive	501753	503273	-1	Protein involved in DNA repair	Protein involved in DNA repair; related to the human CSA protein that is involved in transcription-coupled repair nucleotide excision repair
S000002438	YDR031W	MIX14	Mitochondrial Intermembrane space CX(n)C motif protein	503498	503863	1	Mitochondrial intermembrane space protein of unknown function	Mitochondrial intermembrane space protein of unknown function; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress
S000002439	YDR032C	PST2	Protoplasts-SecreTed	504099	504695	-1	FMN-dependent NAD(P)H:quinone oxidoreductase	FMN-dependent NAD(P)H:quinone oxidoreductase; induced by oxidative stress in a Yap1p dependent manner; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; PST2 has a paralog, RFS1, that arose from the whole genome duplication
S000002440	YDR033W	MRH1	Membrane protein Related to Hsp30p	508147	509109	1	Protein that localizes primarily to the plasma membrane	Protein that localizes primarily to the plasma membrane; also found at the nuclear envelope; long-lived protein that is asymmetrically retained in the plasma membrane of mother cells; the authentic, non-tagged protein is detected in mitochondria in a phosphorylated state; null mutation confers sensitivity to acetic acid
S000002441	YDR034C	LYS14	LYSine requiring	509737	512109	-1	Transcriptional activator involved in regulating lysine biosynthesis	Transcriptional activator involved in regulating lysine biosynthesis; involved in the regulation of genes of the lysine biosynthesis pathway; requires 2-aminoadipate semialdehyde as co-inducer
S000002442	YDR035W	ARO3	AROmatic amino acid requiring	521816	522928	1	3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase	3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase; catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan
S000002443	YDR036C	EHD3	""	523211	524713	-1	3-hydroxyisobutyryl-CoA hydrolase	3-hydroxyisobutyryl-CoA hydrolase; member of a family of enoyl-CoA hydratase/isomerases; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; phosphorylated; mutation affects fluid-phase endocytosis
S000002444	YDR037W	KRS1	Lysyl (K) tRNA Synthetase	525440	527215	1	Lysyl-tRNA synthetase	Lysyl-tRNA synthetase
S000002448	YDR041W	RSM10	Ribosomal Small subunit of Mitochondria	539803	540414	1	Mitochondrial ribosomal protein of the small subunit	Mitochondrial ribosomal protein of the small subunit; has similarity to E. coli S10 ribosomal protein; essential for viability, unlike most other mitoribosomal proteins
S000002449	YDR042C	""	""	540601	541203	-1	Putative protein of unknown function	Putative protein of unknown function; expression is increased in ssu72-ts69 mutant
S000002450	YDR043C	NRG1	Negative Regulator of Glucose-repressed genes	542674	543369	-1	Transcriptional repressor	Transcriptional repressor; recruits the Cyc8p-Tup1p complex to promoters; mediates glucose repression and negatively regulates a variety of processes including filamentous growth and alkaline pH response; activated in stochastic pulses of nuclear localization in response to low glucose
S000002451	YDR044W	HEM13	HEMe biosynthesis	546642	547628	1	Coproporphyrinogen III oxidase	Coproporphyrinogen III oxidase; oxygen-requiring enzyme that catalyzes sixth step in heme biosynthetic pathway; transcription is repressed by oxygen and heme (via Rox1p and Hap1p); human homolog CPOX can complement yeast mutant and allow growth of haploid null after sporulation of a heterozygous diploid
S000002452	YDR045C	RPC11	RNA Polymerase C	547978	548310	-1	RNA polymerase III subunit C11	RNA polymerase III subunit C11; mediates pol III RNA cleavage activity and is important for termination of transcription; homologous to TFIIS
S000002453	YDR046C	BAP3	Branched-chain Amino acid Permease	548762	550576	-1	Amino acid permease	Amino acid permease; involved in uptake of cysteine, leucine, isoleucine and valine; BAP3 has a paralog, BAP2, that arose from the whole genome duplication
S000002454	YDR047W	HEM12	HEMe biosynthesis	551860	552948	1	Uroporphyrinogen decarboxylase	Uroporphyrinogen decarboxylase; catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda
S000002455	YDR048C	""	""	553084	553398	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps ORF VMS1/YDR049W
S000002456	YDR049W	VMS1	VCP/Cdc48-associated Mitochondrial Stress-responsive	553254	555152	1	Peptidyl-tRNA hydrolase that releases stalled peptides from ribosomes,	Peptidyl-tRNA hydrolase that releases stalled peptides from ribosomes, component of a Cdc48p-complex; involved in protein quality control; exhibits cytosolic and ER-membrane localization; contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; translocates to mitochondria under oxidative stress and forms a complex with Cdc48p and Npl4p that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD)
S000002457	YDR050C	TPI1	Triose-Phosphate Isomerase	555726	556472	-1	Triose phosphate isomerase, abundant glycolytic enzyme	Triose phosphate isomerase, abundant glycolytic enzyme; mRNA half-life is regulated by iron availability; transcription is controlled by activators Reb1p, Gcr1p, and Rap1p through binding sites in the 5' non-coding region; inhibition of Tpi1p activity by PEP (phosphoenolpyruvate) stimulates redox metabolism in respiring cells; E104D mutation in human homolog TPI1 causes a rare autosomal disease; human TPI1 can complement yeast null mutant
S000002458	YDR051C	DET1	Decreased Ergosterol Transport	557056	558060	-1	Acid phosphatase	Acid phosphatase; involved in the non-vesicular transport of sterols in both directions between the endoplasmic reticulum and plasma membrane; deletion confers sensitivity to nickel
S000002459	YDR052C	DBF4	DumbBell Former	558511	560625	-1	Regulatory subunit of Cdc7p-Dbf4p kinase complex	Regulatory subunit of Cdc7p-Dbf4p kinase complex; required for Cdc7p kinase activity and initiation of DNA replication; phosphorylates the Mcm2-7 family of proteins; cell cycle regulated; relative distribution to the nucleus increases upon DNA replication stress; co-expression of human CDC7 and DBF4 complements single cdc7 or dbf4 null mutations or the cdc7 dbf4 double null mutation
S000002460	YDR053W	""	""	560249	560644	1	Putative protein of unknown function	Putative protein of unknown function; open reading frame overlaps 5' end of essential DBF4 gene encoding the regulatory subunit of the Cdc7p-Dbf4p kinase complex
S000002461	YDR054C	CDC34	Cell Division Cycle	561440	562327	-1	Ubiquitin-conjugating enzyme (E2)	Ubiquitin-conjugating enzyme (E2); catalytic subunit of SCF ubiquitin-protein ligase complex (together with Skp1p, Rbx1p, Cdc53p, and an F-box protein) that regulates cell cycle progression by targeting key substrates for degradation; protein abundance increases in response to DNA replication stress; human CDC34 functionally complements the thermosensitivity of the cdc34-2 mutant
S000002462	YDR055W	PST1	Protoplasts-SecreTed	563527	564861	1	Cell wall protein that contains a putative GPI-attachment site	Cell wall protein that contains a putative GPI-attachment site; secreted by regenerating protoplasts; up-regulated by activation of the cell integrity pathway, as mediated by Rlm1p; upregulated by cell wall damage via disruption of FKS1; PST1 has a paralog, ECM33, that arose from the whole genome duplication
S000002463	YDR056C	EMC10	ER Membrane protein Complex	565023	565640	-1	Putative protein of unknown function	Putative protein of unknown function; subunit of evolutionarily conserved EMC (Endoplasmic Reticulum Membrane Complex) implicated in ERAD (ER-associated degradation) and proper assembly of multi-pass transmembrane (TM) proteins; EMC acts in yeast as an ER-mitochondria tether that interacts with outer membrane protein Tom5p of TOM (Translocase of the Mitochondrial Outer Membrane) complex; YDR056C is not an essential protein
S000002464	YDR057W	YOS9	Yeast OS-9 homolog	565927	567555	1	ER quality-control lectin	ER quality-control lectin; integral subunit of the HRD ligase; participates in efficient ER retention of misfolded proteins by recognizing them and delivering them to Hrd1p; binds to glycans with terminal alpha-1,6 linked mannose on misfolded N-glycosylated proteins and participates in targeting proteins to ERAD; member of the OS-9 protein family
S000002465	YDR058C	TGL2	TriacylGlycerol Lipase	567723	568703	-1	Triacylglycerol lipase that is localized to the mitochondria	Triacylglycerol lipase that is localized to the mitochondria; has lipolytic activity towards triacylglycerols and diacylglycerols when expressed in E. coli
S000002466	YDR059C	UBC5	UBiquitin-Conjugating	569234	569770	-1	Ubiquitin-conjugating enzyme	Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived, abnormal, or excess proteins, including histone H3; central component of the cellular stress response; expression is heat inducible; protein abundance increases in response to DNA replication stress; UBC5 has a paralog, UBC4, that arose from the whole genome duplication
S000002467	YDR060W	MAK21	MAintenance of Killer	570649	573726	1	Constituent of 66S pre-ribosomal particles	Constituent of 66S pre-ribosomal particles; required for large (60S) ribosomal subunit biogenesis; acts as part of a Mak21p-Noc2p-Rrp5p module that associates with nascent pre-rRNA during transcription and has a role in bigenesis of the large ribosomal subunit; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein
S000002468	YDR061W	""	""	574164	575783	1	Protein with similarity to ABC transporter family members	Protein with similarity to ABC transporter family members; lacks predicted membrane-spanning regions; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance
S000002469	YDR062W	LCB2	Long-Chain Base	576474	578159	1	Component of serine palmitoyltransferase	Component of serine palmitoyltransferase; responsible along with Lcb1p for the first committed step in sphingolipid synthesis, which is the condensation of serine with palmitoyl-CoA to form 3-ketosphinganine
S000002470	YDR063W	AIM7	Altered Inheritance rate of Mitochondria	578663	579112	1	Protein that interacts with Arp2/3 complex	Protein that interacts with Arp2/3 complex; interacts with Arp2/3 complex to stimulate actin filament debranching and inhibit actin nucleation; has similarity to Cof1p and also to human glia maturation factor (GMF); null mutant displays elevated mitochondrial genome loss
S000002472	YDR065W	RRG1	Required for Respiratory Growth	580687	581784	1	Protein of unknown function	Protein of unknown function; required for efficient 5' processing of mitochondrial tRNAs, for respiratory growth and mitochondrial genome maintenance; required for vacuolar acidification; localizes to the matrix side of the inner mitochondrial membrane
S000002473	YDR066C	RTR2	Regulator of TRanscription	581908	582498	-1	Protein of unknown function	Protein of unknown function; exhibits genetic interactions with Rtr1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YDR066C is not an essential gene; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress; RTR2 has a paralog, RTR1, that arose from the whole genome duplication
S000002474	YDR067C	OCA6	Oxidant-induced Cell-cycle Arrest	582791	583465	-1	Cytoplasmic protein required for replication of Brome mosaic virus	Cytoplasmic protein required for replication of Brome mosaic virus; S. cerevisiae is a model system for studying positive-strand RNA virus replication; null mutation confers sensitivity to tunicamycin and DTT
S000002475	YDR068W	DOS2	""	583713	584645	1	Protein of unknown function	Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
S000002476	YDR069C	DOA4	Degradation Of Alpha	584939	587719	-1	Ubiquitin hydrolase	Ubiquitin hydrolase; deubiquitinates intralumenal vesicle (ILVs) cargo proteins; required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins destined for the vacuole; DOA4 has a paralog, UBP5, that arose from the whole genome duplication
S000002477	YDR070C	FMP16	Found in Mitochondrial Proteome	588098	588379	-1	Protein of unknown function	Protein of unknown function; may be involved in responding to conditions of stress; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress
S000002478	YDR071C	PAA1	PolyAmine Acetyltransferase	588827	589402	-1	Polyamine acetyltransferase	Polyamine acetyltransferase; acetylates polyamines (e.g. putrescine, spermidine, spermine) and also aralkylamines (e.g. tryptamine, phenylethylamine); may be involved in transcription and/or DNA replication
S000002479	YDR072C	IPT1	InositolPhosphoTransferase	589761	591344	-1	Inositolphosphotransferase	Inositolphosphotransferase; involved in synthesis of mannose-(inositol-P)2-ceramide (M(IP)2C), the most abundant sphingolipid; can mutate to resistance to the antifungals syringomycin E and DmAMP1 and to K. lactis zymocin
S000002480	YDR073W	SNF11	Sucrose NonFermenting	592439	592948	1	Subunit of the SWI/SNF chromatin remodeling complex	Subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; interacts with a highly conserved 40-residue sequence of Snf2p; relocates to the cytosol under hypoxic conditions
S000002481	YDR074W	TPS2	Trehalose-6-Phosphate Synthase/phosphatase	593893	596583	1	Phosphatase subunit of the trehalose-6-P synthase/phosphatase complex	Phosphatase subunit of the trehalose-6-P synthase/phosphatase complex; involved in the synthesis of storage carbohydrate trehalose; positive regulator of autophagy involved in the dephosphorylation of Rim15p leading to ATG8 induction; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; protein abundance increases in response to DNA replication stress
S000002482	YDR075W	PPH3	Protein PHosphatase	597156	598082	1	Catalytic subunit of protein phosphatase PP4 complex	Catalytic subunit of protein phosphatase PP4 complex; Pph3p and Psy2p form active complex, Psy4p may provide substrate specificity; regulates recovery from the DNA damage checkpoint, the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair and efficient Non-Homologous End-Joining (NHEJ) pathway; involved in activation of Gln3p to alleviate nitrogen catabolite repression; Pph3p and Psy2p localize to foci on meiotic chromosomes
S000002483	YDR076W	RAD55	RADiation sensitive	598468	599688	1	Protein that stimulates strand exchange	Protein that stimulates strand exchange; stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p
S000002484	YDR077W	SED1	Suppression of Exponential Defect	600793	601809	1	Major stress-induced structural GPI-cell wall glycoprotein	Major stress-induced structural GPI-cell wall glycoprotein; associates with translating ribosomes, possible role in mitochondrial genome maintenance; ORF contains two distinct variable minisatellites; SED1 has a paralog, SPI1, that arose from the whole genome duplication
S000002485	YDR078C	SHU2	Suppressor of HydroxyUrea sensitivity	602198	602869	-1	Component of Shu complex (aka PCSS complex)	Component of Shu complex (aka PCSS complex); Shu complex also includes Psy3, Csm2, Shu1, and promotes error-free DNA repair, Shu complex mediates inhibition of Srs2p function; promotes formation of Rad51p filaments; important for both mitotic and meiotic homologous recombination, and contains a conserved SWIM domain that is necessary for both
S000002486	YDR079W	PET100	PETite colonies	603064	603399	1	Chaperone that facilitates the assembly of cytochrome c oxidase	Chaperone that facilitates the assembly of cytochrome c oxidase; integral to the mitochondrial inner membrane; interacts with a subcomplex of subunits VII, VIIa, and VIII (Cox7p, Cox9p, and Cox8p) but not with the holoenzyme
S000002487	YDR080W	VPS41	Vacuolar Protein Sorting	604008	606986	1	Subunit of the HOPS endocytic tethering complex	Subunit of the HOPS endocytic tethering complex; vacuole membrane protein that functions as a Rab GTPase effector, interacting specifically with the GTP-bound conformation of Ypt7p, facilitating tethering, docking and promoting membrane fusion events at the late endosome and vacuole; required for both membrane and protein trafficking; Yck3p-mediated phosphorylation regulates the organization of vacuolar fusion sites
S000002489	YDR082W	STN1	Suppressor of cdc ThirteeN	610441	611925	1	Telomere end-binding and capping protein	Telomere end-binding and capping protein; plays a key role with Pol12p in linking telomerase action with completion of lagging strand synthesis, and in a regulatory step required for telomere capping; similar to human Stn1
S000002490	YDR083W	RRP8	Ribosomal RNA Processing	612073	613251	1	Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase	Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with gar1 mutation; deletion disrupts telomere maintenance by influencing expression of neighboring gene STN1; mammalian homolog nucleomethylin has been linked to nutrient availability signaling pathways and obesity
S000002491	YDR084C	TVP23	Tlg2-Vesicle Protein	613405	614004	-1	Integral membrane protein	Integral membrane protein; localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
S000002492	YDR085C	AFR1	Alpha-Factor Receptor regulator	614288	616150	-1	Protein required for pheromone-induced projection (shmoo) formation	Protein required for pheromone-induced projection (shmoo) formation; regulates septin architecture during mating; has an RVXF motif that mediates targeting of Glc7p to mating projections; also detected in peroxisomes; interacts with Cdc12p; AFR1 has a paralog, YER158C, that arose from the whole genome duplication
S000002494	YDR087C	RRP1	Ribosomal RNA Processing	617470	618306	-1	Essential evolutionarily conserved nucleolar protein	Essential evolutionarily conserved nucleolar protein; necessary for biogenesis of 60S ribosomal subunits and for processing of pre-rRNAs to mature rRNA; associated with several distinct 66S pre-ribosomal particles
S000002495	YDR088C	SLU7	Synergistic Lethal with U5 snRNA	618497	619645	-1	RNA splicing factor	RNA splicing factor; required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain
S000002496	YDR089W	VTC5	Vacuole Transporter Chaperone	622112	624721	1	Novel subunit of the vacuolar transporter chaperone complex	Novel subunit of the vacuolar transporter chaperone complex; vacuolar transmembrane protein that regulates biosynthesis of polyphosphate; deletion reduces and overexpression increases polyP accumulation; SPX domain (Syg1, Pho81, Xpr1)-containing protein involved in phosphate homeostasis; relocalizes from vacuole to cytoplasm upon DNA replication stress
S000002497	YDR090C	ILT1	Ionic Liquid Tolerance	625066	625998	-1	Protein of unknown function	Protein of unknown function; deletion confers sensitivity to cationic compounds; green fluorescent protein (GFP)-fusion protein localizes to the plasma membrane
S000002498	YDR091C	RLI1	RNase L Inhibitor	626708	628534	-1	Essential Fe-S protein	Essential Fe-S protein; required for ribosome biogenesis, translation initiation/termination; facilitates binding of multifactor complex (MFC) of initiation factors to small ribosomal subunit; Dom34-Hbs1 complex and Rli1p work in dissociating inactive ribosomes, thereby facilitating translation restart; forms complex with Lto1p and Yae1p; dependency on ROS-labile FeS clusters, activity in nuclear ribosomal-subunit export impaired by mild oxidative stress
S000002499	YDR092W	UBC13	UBiquitin-Conjugating	629876	630605	1	E2 ubiquitin-conjugating enzyme	E2 ubiquitin-conjugating enzyme; involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus
S000002500	YDR093W	DNF2	Drs2 Neo1 Family	631282	636120	1	Aminophospholipid translocase (flippase)	Aminophospholipid translocase (flippase); type 4 P-type ATPase; involved in phospholipid translocation, contributing to endocytosis, protein transport, and cellular polarization; localizes primarily to the plasma membrane; localizes to the shmoo tip where it has a redundant role in the cellular response to mating pheromone; DNF2 has a paralog, DNF1, that arose from the whole genome duplication
S000002501	YDR094W	""	""	635840	636175	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified ORF DNF2/YDR093W
S000002502	YDR095C	""	""	636117	636527	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000002503	YDR096W	GIS1	GIg1-2 Suppressor	637139	639823	1	Histone demethylase and transcription factor	Histone demethylase and transcription factor; regulates genes during nutrient limitation; activity modulated by proteasome-mediated proteolysis; has JmjC and JmjN domain in N-terminus that interact, promoting stability and proper transcriptional activity; contains two transactivating domains downstream of Jmj domains and a C-terminal DNA binding domain; relocalizes to the cytosol in response to hypoxia; GIS1 has a paralog, RPH1, that arose from the whole genome duplication
S000002504	YDR097C	MSH6	MutS Homolog	640109	643837	-1	Protein required for mismatch repair in mitosis and meiosis	Protein required for mismatch repair in mitosis and meiosis; forms a complex with Msh2p to repair both single-base & insertion-deletion mispairs; also involved in interstrand cross-link repair; potentially phosphorylated by Cdc28p; contains PIP motif that binds PCNA (Pol30p) and Rev1p
S000002505	YDR098C	GRX3	GlutaRedoXin	644178	644930	-1	Glutathione-dependent oxidoreductase	Glutathione-dependent oxidoreductase; hydroperoxide and superoxide-radical responsive monothiol glutaredoxin subfamily member with Grx4p and Grx5p; redundantly protects cells from oxidative damage along with GRX4 and GRX5; with Grx4p, promotes the dissociation of Aft1p from iron regulon gene promoters and subsequent nuclear export in iron-replete conditions, regulating iron homeostasis; involved with Grx4p in the deglutathionylation of Sir2p, restoring deacetylase activity after disulfide stress
S000002506	YDR099W	BMH2	Brain Modulosignalin Homolog	653607	654428	1	14-3-3 protein, minor isoform	14-3-3 protein, minor isoform; involved in post-transcriptional control of the proteome; binds to both proteins and to DNA, including replication origins; regulates multiple processes including exocytosis, vesicle transport, Ras/MAPK and rapamycin-sensitive signaling and meiotic commitment; protein abundance and relative distribution to the nucleus increase upon DNA replication stress; abundance relative to Bmh1p increases during sporulation; similar to several human 14-3-3 proteins
S000002507	YDR100W	TVP15	Tlg2-Vesicle Protein	655013	655444	1	Integral membrane protein	Integral membrane protein; localized to late Golgi vesicles along with the v-SNARE Tlg2p
S000002508	YDR101C	ARX1	Associated with Ribosomal eXport complex	655686	657467	-1	Nuclear export factor for the ribosomal pre-60S subunit	Nuclear export factor for the ribosomal pre-60S subunit; shuttling factor which directly binds FG rich nucleoporins and facilities translocation through the nuclear pore complex; interacts directly with Alb1p; responsible for Tif6p recycling defects in the absence of Rei1; associated with the ribosomal export complex
S000002509	YDR102C	""	""	657648	657980	-1	Putative protein of unknown function	Putative protein of unknown function; conserved among S. cerevisiae strains; YDR102C is not an essential gene; homozygous diploid deletion strain exhibits high budding index
S000002510	YDR103W	STE5	STErile	658350	661103	1	Pheromone-responsive MAPK scaffold protein	Pheromone-responsive MAPK scaffold protein; couples activation of the G-protein-coupled pheromone receptor to MAPK activation; intramolecular interaction of PH and VWA domains blocks activation of assembled signaling cascade components (Ste11p, Ste7p and Fus3p) under basal conditions; Gbeta-gamma (Ste4p-Ste18p)-dependent docking at the plasma membrane and binding of PI(4,5)P2 by the PH domain relieves autoinhibition, resulting in pheromone-dependent pathway activation
S000002511	YDR104C	SPO71	SPOrulation	661173	664910	-1	Meiosis-specific protein required for prospore membrane morphogenesis	Meiosis-specific protein required for prospore membrane morphogenesis; localizes to the prospore membrane (PSM) during sporulation; required for PSM elongation and closure; genetically antagonistic to SPO1; recruits Vps13p to the PSM during sporulation; interacts and functions cooperatively with Spo73p; mutants have defects in the PSM, aberrant spore wall formation and reduced PtdIns-phosphate pools in the PSM; contains three PH-like domains
S000002512	YDR105C	TMS1	""	665349	666770	-1	Vacuolar membrane protein of unknown function	Vacuolar membrane protein of unknown function; is conserved in mammals; predicted to contain eleven transmembrane helices; interacts with Pdr5p, a protein involved in multidrug resistance
S000002514	YDR107C	TMN2	TransMembrane Nine	669016	671034	-1	Protein with a role in cellular adhesion and filamentous growth	Protein with a role in cellular adhesion and filamentous growth; similar to Tmn3p; member of the evolutionarily conserved Transmembrane Nine family of proteins with nine membrane-spanning segments; TMN2 has a paralog, EMP70, that arose from the whole genome duplication
S000002515	YDR108W	TRS85	TRapp Subunit	671269	673365	1	Component of transport protein particle (TRAPP) complex III	Component of transport protein particle (TRAPP) complex III; TRAPPIII is a multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating endosome-Golgi traffic and required for membrane expansion during autophagy and the CVT pathway; directs Ypt1p to the PAS; late post-replication meiotic role
S000002516	YDR109C	""	""	673520	675667	-1	D-ribulokinase	D-ribulokinase; carbohydrate kinase that specifically converts D-ribulose to D-ribulose 5-phosphate during pentose metabolism; null mutant accumulates D-ribulose and the precursor ribitol; proposed to function as a D-ribulose metabolic repair enzyme; member of the FGGY family of carbohydrate kinases; human homolog, FGGY, is involved in both D-ribulose and ribitol metabolism; human FGGY has been linked to both sporadic amyotrophic lateral sclerosis and bipolar disorder
S000002517	YDR110W	FOB1	FOrk Blocking less	676102	677802	1	Nucleolar protein that binds the rDNA replication fork barrier site	Nucleolar protein that binds the rDNA replication fork barrier site; required for replication fork blocking, recombinational hotspot activity, condensin recruitment to replication fork barrier (RFB), and rDNA repeat segregation; related to retroviral integrases
S000002518	YDR111C	ALT2	ALanine Transaminase	678241	679764	-1	Catalytically inactive alanine transaminase	Catalytically inactive alanine transaminase; expression is repressed in the presence of alanine and repression is mediated by Nrg1p; ALT2 has a paralog, ALT1, that arose from the whole genome duplication
S000002519	YDR112W	IRC2	Increased Recombination Centers	679544	679852	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps ALT2/YDR111C; null mutant displays increased levels of spontaneous Rad52p foci
S000002520	YDR113C	PDS1	Precocious Dissociation of Sisters	680496	681617	-1	Securin	Securin; inhibits anaphase by binding separin Esp1p; blocks cyclin destruction and mitotic exit, essential for meiotic progression and mitotic cell cycle arrest; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation
S000002521	YDR114C	""	""	681924	682226	-1	Putative protein of unknown function	Putative protein of unknown function; deletion mutant exhibits poor growth at elevated pH and calcium
S000002522	YDR115W	MRX14	Mitochondrial oRganization of gene eXpression (MIOREX)	682175	682492	1	Putative mitochondrial ribosomal protein of the large subunit	Putative mitochondrial ribosomal protein of the large subunit; required for respiratory growth and for efficient translation of mitochondrial COX1 and COX3;  conditional mutant has reduced cytochrome c oxidase activity; protein increases in abundance and relocalizes to the plasma membrane upon DNA replication stress; contains and N-terminal mitochondrial targeting sequence; similar to E. coli L34 ribosomal protein
S000002523	YDR116C	MRPL1	Mitochondrial Ribosomal Protein, Large subunit	682724	683581	-1	Mitochondrial ribosomal protein of the large subunit	Mitochondrial ribosomal protein of the large subunit
S000002524	YDR117C	TMA64	Translation Machinery Associated	683946	685643	-1	Protein of unknown function that associates with ribosomes	Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; in mammals the corresponding protein, eIF2D, has been shown to possess translation initiation factor activity
S000002525	YDR118W	APC4	Anaphase Promoting Complex	685882	687840	1	Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C)	Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to the nucleus increases upon DNA replication stress
S000002526	YDR119W	VBA4	Vacuolar Basic Amino acid transporter	688227	690533	1	Protein of unknown function	Protein of unknown function; proposed role as basic amino acid permease based on phylogeny; targeted to vacuole via AP-3 pathway; physical interaction with Atg27p suggests possible role in autophagy; non-essential gene
S000002527	YDR120C	TRM1	tRNA Methyltransferase	691549	693261	-1	tRNA methyltransferase	tRNA methyltransferase; produces two isoforms based on alternative translational starts; localizes to the nucleus and mitochondrion, producing N2,N2-dimethylguanosine in tRNAs; involved in the methylation of guanosine and uridine ribonucleosides in mRNA; nuclear Trm1p is evenly distributed around the inner nuclear membrane in WT, but mislocalizes as puncta near ER-nucleus junctions in spindle pole body mutants; both Trm1p inner nuclear membrane targeting and maintenance depend upon the SPB
S000002528	YDR121W	DPB4	DNA Polymerase B (II) subunit	693585	694175	1	Subunit of DNA pol epsilon and of ISW2 chromatin accessibility complex	Subunit of DNA pol epsilon and of ISW2 chromatin accessibility complex; involved in both chromosomal DNA replication and inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; interacts with extranucleosomal DNA and acts as anchor point for ISW2 complex that retains its position on DNA during nucleosome mobilization
S000002529	YDR122W	KIN1	KINase	694700	697894	1	S/T protein kinase	S/T protein kinase; regulates polarized exocytosis and the Ire1p-mediated unfolded protein response; regulates HAC1 mRNA translocation, splicing and translation with KIN2 during ER stress; direct phosphorylation of the substrate Sec9p (S190) is enhanced by prior substrate priming (S192); localizes to the cytoplasmic face of the plasma membrane; activation loop phosphorylation (T302) required for full kinase activity; orthologous to MARK/PAR-1, AMP-activated protein kinase (AMPK) family members
S000002530	YDR123C	INO2	INOsitol requiring	698554	699468	-1	Transcription factor	Transcription factor; component of the heteromeric Ino2p/Ino4p basic helix-loop-helix transcription activator that binds inositol/choline-responsive elements (ICREs), required for derepression of phospholipid biosynthetic genes in response to inositol depletion; involved in diauxic shift
S000002531	YDR124W	""	""	700315	701289	1	Mitochondrial protein of unknown function	Mitochondrial protein of unknown function; null mutant show severe fragmentation of mitochondria; expression is strongly induced by alpha factor; may have role in maintenance of mitochondrial morphology
S000002532	YDR125C	ECM18	ExtraCellular Mutant	701397	702758	-1	Protein of unknown function	Protein of unknown function; ECM18 has a paralog, ICT1, that arose from the whole genome duplication
S000002533	YDR126W	SWF1	Spore Wall Formation	703234	704244	1	Palmitoyltransferase that acts on transmembrane proteins	Palmitoyltransferase that acts on transmembrane proteins; including the SNAREs Snc1p, Syn8p, Tlg1p and likely all SNAREs; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; may have a role in vacuole fusion
S000002534	YDR127W	ARO1	AROmatic amino acid requiring	704484	709250	1	Pentafunctional arom protein	Pentafunctional arom protein; catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
S000002535	YDR128W	MTC5	Maintenance of Telomere Capping	709549	712995	1	Subunit of SEACAT, a subcomplex of the SEA complex	Subunit of SEACAT, a subcomplex of the SEA complex; Mtc1p, along with Rtc1p and Sea4p, redundantly inhibit the TORC1 inhibitory role of the Iml1p/SEACIT (Iml1p-Npr2p-Npr3p) subcomplex, a GAP for GTPase Gtr1p (EGOC subunit) in response to amino acid limitation, thereby resulting in activation of TORC1 signaling; SEA is a coatomer-related complex that associates dynamically with the vacuole; relative distribution to the vacuolar membrane decreases upon DNA replication stress
S000002536	YDR129C	SAC6	Suppressor of ACtin	713340	715379	-1	Fimbrin, actin-bundling protein	Fimbrin, actin-bundling protein; cooperates with Scp1p in organization and maintenance of the actin cytoskeleton; phosphorylated by Cdc28p/Clb2p in metaphase on T103, to regulate conformation, and modulate actin filament binding affinity and actin cable dynamics; relocalizes from the plasma membrane to the cytoplasm upon DNA replication stress; human homologs PLS3 and LCP1 implicated in spinocerebellar ataxia type 2 (SCA2) can each complement yeast null mutant
S000002537	YDR130C	FIN1	Filaments In between Nuclei	715747	716622	-1	Spindle pole body-related intermediate filament protein	Spindle pole body-related intermediate filament protein; forms cell cycle-specific filaments between spindle pole bodies in dividing cells; localizes to poles and microtubules of spindle during anaphase and contributes to spindle stability; involved in Glc7p localization and regulation; relative distribution to the nucleus increases upon DNA replication stress
S000002538	YDR131C	""	""	716790	718460	-1	F-box protein subunit of SCF ubiquitin ligase complex	F-box protein subunit of SCF ubiquitin ligase complex; substrate-specific adaptor subunit that recruits substrates to a core ubiquitination complex
S000002539	YDR132C	MRX16	Mitochondrial oRganization of gene eXpression (MIOREX)	718816	720303	-1	Protein that associates with the large mitoribosomal subunit	Protein that associates with the large mitoribosomal subunit; abundance and relative nuclear distribution to the protein increases upon DNA replication stress; YDR132C has a paralog, YLR108C, that arose from the whole genome duplication
S000002540	YDR133C	""	""	720962	721297	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps YDR134C
S000002541	YDR134C	CCW22	Covalently bound Cell Wall protein	721071	721481	-1	Cell wall protein	Cell wall protein; YDR134C has a paralog, CCW12, that arose from the whole genome duplication; S. cerevisiae genome reference strain S288C contains an internal in-frame stop at codon 67, which in other strains encodes glutamine
S000002542	YDR135C	YCF1	Yeast Cadmium Factor	723004	727551	-1	Vacuolar glutathione S-conjugate transporter	Vacuolar glutathione S-conjugate transporter; ABC-C transporter of the ATP-binding cassette family; required for vacuole fusion; forms stable complexes with vacuole fusion machinery; regulates Vam7p recruitment to vacuoles; role in detoxifying metals (Cd, Hg, As); transports GSSG that is not immediately reduced in cytosol to vacuole; transports unconjugated bilirubin, selenodigluthatione, oxidized glutathione; similar to human cystic fibrosis protein CFTR
S000002543	YDR136C	VPS61	Vacuolar Protein Sorting	727711	728283	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect
S000002544	YDR137W	RGP1	Reduced Growth Phenotype	728259	730250	1	Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p)	Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p); this complex catalyzes nucleotide exchange on Ypt6p
S000002545	YDR138W	HPR1	HyPerRecombination	730578	732836	1	Subunit of THO/TREX complexes	Subunit of THO/TREX complexes; this complex couple transcription elongation with mitotic recombination and with mRNA metabolism and export, subunit of an RNA Pol II complex; regulates lifespan; involved in telomere maintenance; similar to Top1p
S000002546	YDR139C	RUB1	Related to UBiquitin	733618	733924	-1	Ubiquitin-like protein with similarity to mammalian NEDD8	Ubiquitin-like protein with similarity to mammalian NEDD8; conjugation (neddylation) substrates include the cullins Cdc53p, Rtt101p, and Cul3p; activated by Ula1p and Uba3p (E1 enzyme pair); conjugation mediated by Ubc12p (E2 enzyme)
S000002547	YDR140W	MTQ2	Methyltransferase	734138	734803	1	S-adenosylmethionine-dependent methyltransferase	S-adenosylmethionine-dependent methyltransferase; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC; member of the seven beta-strand family
S000002548	YDR141C	DOP1	homolog of A. nidulans DOPey	734901	739997	-1	Protein involved in vesicular transport at trans-Golgi network (TGN)	Protein involved in vesicular transport at trans-Golgi network (TGN); TGN-localized, leucine-zipper domain protein; involved in endosome-to-Golgi transport during endocytic recycling, and retrograde transport of glycosyltransferases from the TGN to the Golgi; involved in organization of the ER, establishment of cell polarity, and morphogenesis; detected in highly purified mitochondria in high-throughput studies
S000002549	YDR142C	PEX7	PEroXin	740473	741600	-1	Peroxisomal signal receptor for peroxisomal matrix proteins	Peroxisomal signal receptor for peroxisomal matrix proteins; recognizes the N-terminal nonapeptide signal (PTS2); WD repeat protein; defects in human homolog cause lethal rhizomelic chondrodysplasia punctata (RCDP)
S000002550	YDR143C	SAN1	Sir Antagonist	742042	743874	-1	Ubiquitin-protein ligase	Ubiquitin-protein ligase; role in proteasome-dependent degradation of aberrant nuclear proteins; targets substrates with regions of exposed hydrophobicity; contains intrinsically disordered regions involved in substrate recognition; prefers a window of exposed hydrophobicity that causes a particular level of protein insolubility, suggesting that San1p evolved to target highly aggregation-prone proteins; localizes to the cytoplasm, nucleus and peroxisomes
S000002551	YDR144C	MKC7	Multicopy suppressor of Kex2 Cold sensitivity	744311	746101	-1	GPI-anchored aspartyl protease	GPI-anchored aspartyl protease; member of the yapsin family of proteases involved in cell wall growth and maintenance; shares functions with Yap3p and Kex2p; MKC7 has a paralog, YPS1, that arose from the whole genome duplication
S000002552	YDR145W	TAF12	TATA binding protein-Associated Factor	746738	748357	1	Subunit (61/68 kDa) of TFIID and SAGA complexes	Subunit (61/68 kDa) of TFIID and SAGA complexes; involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H2A; overexpression of the human ortholog, TAF12, an oncogene involved in the formation of choroid plexus carcinomas, results in dosage chromosomal instability (dCIN) in a human cell line similar to the dCIN observed in yeast overexpressors
S000002553	YDR146C	SWI5	SWItching deficient	748613	750742	-1	Transcription factor that recruits Mediator and Swi/Snf complexes	Transcription factor that recruits Mediator and Swi/Snf complexes; activates transcription of genes expressed at the M/G1 phase boundary and in G1 phase; required for expression of the HO gene controlling mating type switching; localization to nucleus occurs during G1 and appears to be regulated by phosphorylation by Cdc28p kinase; SWI5 has a paralog, ACE2, that arose from the whole genome duplication
S000002554	YDR147W	EKI1	Ethanolamine KInase	751631	753235	1	Ethanolamine kinase	Ethanolamine kinase; primarily responsible for phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; exhibits some choline kinase activity, thus contributing to phosphatidylcholine synthesis via the CDP-choline pathway; EKI1 has a paralog, CKI1, that arose from the whole genome duplication
S000002555	YDR148C	KGD2	alpha-KetoGlutarate Dehydrogenase	753675	755066	-1	Dihydrolipoyl transsuccinylase	Dihydrolipoyl transsuccinylase; component of the mitochondrial alpha-ketoglutarate dehydrogenase complex, which catalyzes the oxidative decarboxylation of alpha-ketoglutarate to succinyl-CoA in the TCA cycle; phosphorylated
S000002556	YDR149C	""	""	755555	756262	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene NUM1/YDR150W; null mutation blocks anaerobic growth
S000002557	YDR150W	NUM1	NUclear Migration	755628	763874	1	Protein required for nuclear migration	Protein required for nuclear migration; component of the mitochondria-ER-cortex-anchor (MECA); required for the association of mitochondria with the cell cortex and for accurate distribution of mitochondrial network; interacts with Mdm36p to link the ER and mitochondria at the cortex; localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex
S000002558	YDR151C	CTH1	Cysteine-Three-Histidine	764178	765155	-1	mRNA binding protein, member of the CCCH zinc finger family	mRNA binding protein, member of the CCCH zinc finger family; similar to mammalian Tis11 protein, which activates transcription and also has a role in mRNA degradation; functions with Tis11p in regulation of iron homeostasis; CTH1 has a paralog, TIS11, that arose from the whole genome duplication
S000002559	YDR152W	GIR2	Genetically Interacts with Ribosomal genes	765706	766503	1	Highly-acidic RWD domain-containing cytoplasmic protein	Highly-acidic RWD domain-containing cytoplasmic protein; forms a highly conserved complex with Rbg2p that is responsible for efficient cell growth under amino acid starvation and binds translational activator Gcn1p in dose-dependent manner according to stress level; associates with translating ribosomes; intrinsically unstructured protein whose stability is enhanced upon binding Rbg2p
S000002560	YDR153C	ENT5	Epsin N-Terminal homology	766736	767971	-1	Protein containing an N-terminal epsin-like domain	Protein containing an N-terminal epsin-like domain; involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p, clathrin adaptor complex AP-1, and clathrin
S000002561	YDR154C	""	""	768403	768753	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; null mutant exhibits synthetic phenotype with alpha-synuclein
S000002562	YDR155C	CPR1	Cyclosporin A-sensitive Proline Rotamase	768512	769000	-1	Cytoplasmic peptidyl-prolyl cis-trans isomerase (cyclophilin)	Cytoplasmic peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds the drug cyclosporin A; N-terminally propionylated in vivo; protein abundance increases in response to DNA replication stress
S000002563	YDR156W	RPA14	RNA Polymerase A	769525	769938	1	RNA polymerase I subunit A14	RNA polymerase I subunit A14
S000002564	YDR157W	""	""	769931	770332	1	Putative protein of unknown function	Putative protein of unknown function; conserved across S. cerevisiae strains
S000002565	YDR158W	HOM2	HOMoserine requiring	770357	771454	1	Aspartic beta semi-aldehyde dehydrogenase	Aspartic beta semi-aldehyde dehydrogenase; catalyzes the second step in the common pathway for methionine and threonine biosynthesis; expression regulated by Gcn4p and the general control of amino acid synthesis
S000002566	YDR159W	SAC3	Suppressor of ACtin	771877	775782	1	mRNA export factor	mRNA export factor; required for biogenesis of the small ribosomal subunit; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; similar to the human germinal center-associated nuclear protein (GANP)
S000002567	YDR160W	SSY1	Sulfonylurea Sensitive on YPD	776163	778721	1	Component of the SPS plasma membrane amino acid sensor system	Component of the SPS plasma membrane amino acid sensor system; senses external amino acid concentration and transmits intracellular signals that result in regulation of expression of amino acid permease genes; other members are Ssy1p, Ptr3p, and Ssy5p
S000002568	YDR161W	ACL4	Assembly Chaperone of RpL4	779043	780206	1	Specific assembly chaperone for ribosomal protein Rpl4a/Rpl4b	Specific assembly chaperone for ribosomal protein Rpl4a/Rpl4b; associates co-translationally with a evolutionarily conserved internal loop of nascent Rpl4a/b, and is released only after an the eukaryotic-specific extension of nascent Rpl4a/b mediates nuclear import, incorporation into the pre-ribosome and complex disassembly; role in biogenesis of the 60S ribosomal subunit; transcriptionally co-regulated with rRNA and ribosome biosynthesis genes
S000002569	YDR162C	NBP2	Nap1 Binding Protein	780390	781100	-1	Protein involved in the HOG (high osmolarity glycerol) pathway	Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p to the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm; contains an SH3 domain and a Ptc1p binding domain (PBM)
S000002570	YDR163W	CWC15	Complexed With Cef1p	781423	781950	1	Non-essential protein involved in pre-mRNA splicing	Non-essential protein involved in pre-mRNA splicing; component of a complex containing Cef1p; has similarity to S. pombe Cwf15p
S000002571	YDR164C	SEC1	SECretory	782041	784215	-1	Sm-like protein involved in docking and fusion of exocytic vesicles	Sm-like protein involved in docking and fusion of exocytic vesicles; binds to assembled SNARE complexes at the membrane and stimulates membrane fusion; localization to sites of secretion (bud neck and bud tip) is dependent on SNARE function; interacts directly with essential exocyst subunit Sec6p
S000002572	YDR165W	TRM82	Transfer RNA Methyltransferase	784871	786205	1	Noncatalytic subunit of a tRNA methyltransferase complex	Noncatalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p; relocalizes to the cytosol in response to hypoxia; mutation in human ortholog WDR4 causes microcephalic primordial dwarfism
S000002573	YDR166C	SEC5	SECretory	786306	789221	-1	Essential 107kDa subunit of the exocyst complex	Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip
S000002574	YDR167W	TAF10	TATA binding protein-Associated Factor	789449	790069	1	Subunit (145 kDa) of TFIID and SAGA complexes	Subunit (145 kDa) of TFIID and SAGA complexes; involved in RNA polymerase II transcription initiation and in chromatin modification
S000002575	YDR168W	CDC37	Cell Division Cycle	790328	791848	1	Essential Hsp90p co-chaperone	Essential Hsp90p co-chaperone; necessary for passage through the START phase of the cell cycle; stabilizes protein kinase nascent chains and participates along with Hsp90p in their folding
S000002576	YDR169C	STB3	Sin Three Binding protein	792348	793889	-1	Ribosomal RNA processing element (RRPE)-binding protein	Ribosomal RNA processing element (RRPE)-binding protein; involved in the glucose-induced transition from quiescence to growth; restricted to nucleus in quiescent cells, released into cytoplasm after glucose repletion; binds Sin3p; relative distribution to the nucleus increases upon DNA replication stress
S000002577	YDR170C	SEC7	SECretory	796193	802222	-1	Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors	Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors; involved in intra-Golgi transport, ER-to-Golgi transport and proliferation of the Golgi; role in directing misfolded proteins to insoluble deposition sites and detoxifying neurological disease proteins; found in the cytoplasm and on Golgi-associated coated vesicles
S000002578	YDR171W	HSP42	Heat Shock Protein	806621	807748	1	Small heat shock protein (sHSP) with chaperone activity	Small heat shock protein (sHSP) with chaperone activity; forms barrel-shaped oligomers that suppress unfolded protein aggregation; involved in cytoskeleton reorganization after heat shock; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress
S000002579	YDR172W	SUP35	SUPpressor	808324	810381	1	Translation termination factor eRF3	Translation termination factor eRF3; has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that modifies cellular fitness, alters translational fidelity by affecting reading frame selection, and results in a nonsense suppressor phenotype; many stress-response genes are repressed in the presence of [PSI(+)]
S000002580	YDR173C	ARG82	ARGinine requiring	810565	811632	-1	Inositol polyphosphate multikinase (IPMK)	Inositol polyphosphate multikinase (IPMK); sequentially phosphorylates Ins(1,4,5)P3 to form Ins(1,3,4,5,6)P5; also has diphosphoinositol polyphosphate synthase activity; regulates genes responsive to arginine, phosphate, and nitrogen; needed by most [PSI+] variants for prion propagation
S000002581	YDR174W	HMO1	High MObility group (HMG) family	812110	812850	1	Chromatin-associated high mobility group (HMG) family member	Chromatin-associated high mobility group (HMG) family member; role in compacting, bending, bridging and looping DNA; lysine-rich CTD is essential for chromatin assembly, forming condensates with dsDNA; functionally similar to linker histone H1; rDNA-binding component regulates transcription from RNAPI promoters; regulates start site selection of ribosomal protein genes via RNAPII promoters; role in genome maintenance; associates with a 5'-3' DNA helicase and Fpr1p
S000002582	YDR175C	RSM24	Ribosomal Small subunit of Mitochondria	813193	814152	-1	Mitochondrial ribosomal protein of the small subunit	Mitochondrial ribosomal protein of the small subunit
S000002583	YDR176W	NGG1	""	814452	816560	1	Subunit of chromatin modifying histone acetyltransferase complexes	Subunit of chromatin modifying histone acetyltransferase complexes; member of the ADA complex, the SAGA complex, and the SLIK complex; transcriptional regulator involved in glucose repression of Gal4p-regulated genes
S000002584	YDR177W	UBC1	UBiquitin-Conjugating	816878	817525	1	Ubiquitin-conjugating enzyme	Ubiquitin-conjugating enzyme; key E2 partner with Ubc4p for the anaphase-promoting complex (APC); mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress key E2 partner with Ubc4p for the anaphase-promoting complex (APC)
S000002585	YDR178W	SDH4	Succinate DeHydrogenase	817950	818495	1	Membrane anchor subunit of succinate dehydrogenase (SDH)	Membrane anchor subunit of succinate dehydrogenase (SDH); involved in coupling the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; has similarity to human SDH subunit D (SDHD), which is implicated in paraganglioma
S000002586	YDR179C	CSN9	Cop9 SigNalosome subunit	818708	819196	-1	Subunit of the Cop9 signalosome	Subunit of the Cop9 signalosome; Cop9 signalosome is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling
S000002587	YDR179W-A	NVJ3	Nucleus-Vacuole Junction	819433	820824	1	Protein with a potential role in tethering ER and vacuoles	Protein with a potential role in tethering ER and vacuoles; localizes to nucleus-vacuole junctions in an Mdm1p-dependent manner; contains a lipid-binding PXA domain
S000002588	YDR180W	SCC2	Sister Chromatid Cohesion	821295	825776	1	Subunit of cohesin loading factor (Scc2p-Scc4p)	Subunit of cohesin loading factor (Scc2p-Scc4p); a complex required for loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX; promotes gene expression program that supports translational fidelity; evolutionarily-conserved adherin; relocalizes to cytosol in response to hypoxia; human disorder Cornelia de Lange syndrome is caused by mutations in NIPBL, the human ortholog of SCC2; conserved role in NHEJ
S000002589	YDR181C	SAS4	Something About Silencing	825910	827355	-1	Subunit of the SAS complex (Sas2p, Sas4p, Sas5p)	Subunit of the SAS complex (Sas2p, Sas4p, Sas5p); acetylates free histones and nucleosomes and regulates transcriptional silencing; required for the HAT activity of Sas2p
S000002590	YDR182W	CDC1	Cell Division Cycle	827582	829057	1	Putative mannose-ethanolamine phosphate phosphodiesterase	Putative mannose-ethanolamine phosphate phosphodiesterase; involved in GPI-anchor remodeling prior to the attachment of cell wall proteins to beta 1,3-glucan, removing ethanolamine phosphate from the first mannose of GPI anchors; mutants display elevated Ca2+-dependent signaling resulting in secondary actin polarization and Golgi inheritance defects; enzyme is Mn2+-dependent; mutants have cell division cycle defect and fragile cell walls
S000002591	YDR183W	PLP1	Phosducin-Like Protein	829585	830277	1	Protein that interacts with CCT (chaperonin containing TCP-1) complex	Protein that interacts with CCT (chaperonin containing TCP-1) complex; has a role in actin and tubulin folding; has weak similarity to phosducins, which are G-protein regulators
S000002592	YDR184C	ATC1	Aip Three Complex	830629	831513	-1	Nuclear protein	Nuclear protein; possibly involved in regulation of cation stress responses and/or in the establishment of bipolar budding pattern; relative distribution to the nucleus decreases upon DNA replication stress
S000002593	YDR185C	UPS3	UnProceSsed	831934	832473	-1	Mitochondrial protein of unknown function	Mitochondrial protein of unknown function; similar to Ups1p and Ups2p which are involved in regulation of mitochondrial cardiolipin and phosphatidylethanolamine levels; null is viable but interacts synthetically with ups1 and ups2 mutations; UPS3 has a paralog, UPS2, that arose from the whole genome duplication
S000002594	YDR186C	SND1	Srp-iNDependent targeting	832859	835492	-1	Protein involved in SRP-independent targeting of substrates to the ER	Protein involved in SRP-independent targeting of substrates to the ER; component of an alternative ER targeting pathway that has partial functional redundancy with the GET pathway; preference for substrates with downstream transmembrane domains; interacts with Env10p/Snd2p and Pho88p/Snd3p; can compensate for loss of SRP; co-purifies with ribosomes; GFP-fusion protein localizes to the cytoplasm
S000002595	YDR187C	""	""	836228	836746	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF CCT6/YDR188W
S000002597	YDR189W	SLY1	Suppressor of Loss of Ypt1	838392	840392	1	Hydrophilic protein involved in ER/Golgi vesicle trafficking	Hydrophilic protein involved in ER/Golgi vesicle trafficking; SM (Sec1/Munc-18) family protein that binds the tSNARE Sed5p and stimulates its assembly into a trans-SNARE membrane-protein complex
S000002598	YDR190C	RVB1	RuVB-like	840604	841995	-1	ATP-dependent DNA helicase, also known as pontin	ATP-dependent DNA helicase, also known as pontin; member of the AAA+ and RuvB-like protein families; similar to Rvb2p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly
S000002599	YDR191W	HST4	Homolog of SIR Two (SIR2)	842337	843449	1	NAD(+)-dependent protein deacetylase	NAD(+)-dependent protein deacetylase; deacetylation targets are primarily mitochondrial proteins; involved along with Hst3p in silencing at telomeres, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism; accumulates in mitochondria in response to biotin starvation and may link biotin metabolism with energy homeostasis; member of the Sir2 family and may be the functional equivalent of human SIRT3
S000002600	YDR192C	NUP42	NUclear Pore	843569	844861	-1	FG-nucleoporin component of central core of the nuclear pore complex	FG-nucleoporin component of central core of the nuclear pore complex; also part of the nuclear pore complex (NPC) cytoplasmic filaments; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; interacts with Gle1p; human homolog NUP42 can complement yeast mutant
S000002601	YDR193W	""	""	844554	844952	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000002602	YDR194C	MSS116	Mitochondrial Splicing System	845952	847946	-1	Mitochondrial transcription elongation factor	Mitochondrial transcription elongation factor; DEAD-box protein; required for efficient splicing of mitochondrial Group I and II introns; non-polar RNA helicase that also facilities strand annealing; promotes RNA folding by stabilizing an early assembly intermediate
S000002603	YDR195W	REF2	RNA End Formation	848599	850200	1	RNA-binding protein	RNA-binding protein; involved in the cleavage step of mRNA 3'-end formation prior to polyadenylation, and in snoRNA maturation; part of holo-CPF subcomplex APT, which associates with 3'-ends of snoRNA- and mRNA-encoding genes; putative regulatory subunit of type 1 protein phosphatase Glc7p, required for actomyosin ring formation, and for timely dephosphorylation and release of Bnr1p from the division site; relocalizes to the cytosol in response to hypoxia
S000002604	YDR196C	CAB5	""	850274	850999	-1	Subunit of the CoA-Synthesizing Protein Complex (CoA-SPC)	Subunit of the CoA-Synthesizing Protein Complex (CoA-SPC); subunits of this complex are: Cab2p, Cab3p, Cab4p, Cab5p, Sis2p and Vhs3p; probable dephospho-CoA kinase (DPCK) that catalyzes the last step in coenzyme A biosynthesis; involved in histone acylation; null mutant lethality is complemented by human homologs DCAKD and COASY and by E. coli coaE (encoding DPCK); extrinsic component of the mitochondrial outer membrane that also localizes to lipid droplets
S000002605	YDR197W	CBS2	Cytochrome B Synthesis	851228	852397	1	Mitochondrial translational activator of the COB mRNA	Mitochondrial translational activator of the COB mRNA; interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader
S000002606	YDR198C	RKM2	Ribosomal protein lysine (K) Methyltransferase	852533	853972	-1	Ribosomal protein lysine methyltransferase	Ribosomal protein lysine methyltransferase; responsible for trimethylation of the lysine residue at position 3 of Rpl12Ap and Rpl12Bp
S000002607	YDR199W	""	""	854175	854540	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPS64/YDR200C; computationally predicted to have thiol-disulfide oxidoreductase activity
S000002608	YDR200C	VPS64	Vacuolar Protein Sorting	854279	856093	-1	Protein required for cytoplasm to vacuole targeting of proteins	Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance; VPS64 has a paralog, FAR10, that arose from the whole genome duplication
S000002609	YDR201W	SPC19	Spindle Pole Component	856317	856814	1	Essential subunit of Dam1 complex (aka DASH complex)	Essential subunit of Dam1 complex (aka DASH complex); complex couples kinetochores to force produced by MT depolymerization thereby aiding in chromosome segregation; also localized to nuclear side of spindle pole body; SPC19 mRNA level is reduced when downstream gene RAV2 is deleted
S000002610	YDR202C	RAV2	Regulator of (H+)-ATPase in Vacuolar membrane	856898	857953	-1	Subunit of RAVE complex (Rav1p, Rav2p, Skp1p)	Subunit of RAVE complex (Rav1p, Rav2p, Skp1p); the RAVE complex associates with the V1 domain of the vacuolar membrane (H+)-ATPase (V-ATPase) and promotes assembly and reassembly of the holoenzyme
S000002611	YDR203W	""	""	857682	857999	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF RAV2/YDR202C
S000002612	YDR204W	COQ4	COenzyme Q	858137	859144	1	Protein with a role in ubiquinone (Coenzyme Q) biosynthesis	Protein with a role in ubiquinone (Coenzyme Q) biosynthesis; possibly functioning in stabilization of Coq7p; located on matrix face of mitochondrial inner membrane; component of a mitochondrial ubiquinone-synthesizing complex; human homolog COQ4 can complement yeast coq4 null mutant
S000002613	YDR205W	MSC2	Meiotic Sister-Chromatid recombination	859346	861520	1	Endoplasmic reticulum zinc transporter	Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
S000002614	YDR206W	EBS1	Est1-like Bcy1 Suppressor	862054	864708	1	Protein involved in translation inhibition and nonsense-mediated decay	Protein involved in translation inhibition and nonsense-mediated decay; interacts with cap binding protein Cdc33p and with Nam7p; localizes to P-bodies upon glucose starvation; mRNA abundance regulated by mRNA decay factors; EBS1 has a paralog, EST1, that arose from the whole genome duplication
S000002615	YDR207C	UME6	Unscheduled Meiotic gene Expression	865012	867522	-1	Rpd3L histone deacetylase complex subunit	Rpd3L histone deacetylase complex subunit; key transcriptional regulator of early meiotic genes; involved in chromatin remodeling and transcriptional repression via DNA looping; binds URS1 upstream regulatory sequence, represses transcription by recruiting conserved histone deacetylase Rpd3p (through co-repressor Sin3p) and chromatin-remodeling factor Isw2p; couples metabolic responses to nutritional cues with initiation and progression of meiosis
S000002616	YDR208W	MSS4	Multicopy Suppressor of Stt4 mutation	868224	870563	1	Phosphatidylinositol-4-phosphate 5-kinase	Phosphatidylinositol-4-phosphate 5-kinase; involved in actin cytoskeleton organization and cell morphogenesis; multicopy suppressor of stt4 mutation
S000002617	YDR209C	""	""	871036	871449	-1	Putative protein of unknown function	Putative protein of unknown function; conserved among S. cerevisiae strains; YDR209C is not an essential gene; partially overlaps uncharacterized gene YDR210W
S000002618	YDR210W	""	""	871074	871301	1	Predicted tail-anchored plasma membrane protein	Predicted tail-anchored plasma membrane protein; contains a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
S000002619	YDR211W	GCD6	General Control Derepressed	884727	886865	1	Catalytic epsilon subunit of the translation initiation factor eIF2B	Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; mutations in human ortholog cause fatal brain disease childhood ataxia with central nervous system hypomyelination (CACH); forms cytoplasmic foci upon DNA replication stress
S000002620	YDR212W	TCP1	Tailless Complex Polypeptide	887232	888911	1	Alpha subunit of chaperonin-containing T-complex	Alpha subunit of chaperonin-containing T-complex; complex mediates protein folding in the cytosol; involved in actin cytoskeleton maintenance; overexpression in neurons suppresses formation of pathogenic conformations of huntingtin protein
S000002621	YDR213W	UPC2	UPtake Control	889751	892492	1	Sterol regulatory element binding protein	Sterol regulatory element binding protein; induces sterol biosynthetic genes, upon sterol depletion; acts as a sterol sensor, binding ergosterol in sterol rich conditions; relocates from intracellular membranes to perinuclear foci upon sterol depletion; redundant activator of filamentation with ECM22, up-regulating the expression of filamentous growth genes; contains a Zn[2]-Cys[6] binuclear cluster; UPC2 has a paralog, ECM22, that arose from the whole genome duplication
S000002622	YDR214W	AHA1	Activator of Heat shock protein 90 ATPase	892875	893927	1	Co-chaperone that binds Hsp82p and activates its ATPase activity	Co-chaperone that binds Hsp82p and activates its ATPase activity; plays a role in determining prion variants; similar to Hch1p; involved in the nuclear import of Hsp82p after DNA damage; expression is regulated by stresses such as heat shock; protein abundance increases in response to DNA replication stress
S000002623	YDR215C	""	""	894089	894502	-1	Putative protein of unknown function	Putative protein of unknown function; conserved across S. cerevisiae strains; null mutant displays elevated sensitivity to expression of a mutant huntingtin fragment or of alpha-synuclein
S000002624	YDR216W	ADR1	Alcohol Dehydrogenase II synthesis Regulator	895035	899006	1	Carbon source-responsive zinc-finger transcription factor	Carbon source-responsive zinc-finger transcription factor; required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization
S000002625	YDR217C	RAD9	RADiation sensitive	899551	903480	-1	DNA damage-dependent checkpoint protein	DNA damage-dependent checkpoint protein; required for cell-cycle arrest in G1/S, intra-S, and G2/M, plays a role in postreplication repair (PRR) pathway; transmits checkpoint signal by activating Rad53p and Chk1p; protects double-strand breaks from premature resection; multiple cyclin dependent kinase consensus sites and the C-terminal BRCT domain contribute to DNA damage checkpoint activation; Rad9p Chk1 Activating Domain (CAD) is phosphorylated at multiple sites by Cdc28p/Clb2p
S000002626	YDR218C	SPR28	SPorulation Regulated	903781	905052	-1	Sporulation-specific homolog of the CDC3/10/11/12 family of genes	Sporulation-specific homolog of the CDC3/10/11/12 family of genes; meiotic septin expressed at high levels during meiotic divisions and ascospore formation; the yeast CDC3/10/11/12 family is a family of bud neck microfilament genes
S000002627	YDR219C	MFB1	Mitochondria-associated F-Box protein	905455	906852	-1	Mitochondria-associated F-box protein	Mitochondria-associated F-box protein; tether that anchors higher-functioning mitochondria at the mother cell distal tip throughout the cell cycle and at the bud tip prior to cytokinesis; role in maintenance of normal mitochondrial morphology and quality control; interacts with Skp1p through the F-box motif; asymmetrically localized to the mother cell through the cell cycle with some bud tip localization just before cytokinesis; function regulated by the bud site selection machinery
S000002628	YDR220C	""	""	907000	907293	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; null mutant exhibits synthetic phenotype with alpha-synuclein
S000002629	YDR221W	GTB1	Glucosidase Two Beta-subunit	907330	909438	1	Glucosidase II beta subunit, forms a complex with alpha subunit Rot2p	Glucosidase II beta subunit, forms a complex with alpha subunit Rot2p; involved in removal of two glucose residues from N-linked glycans during glycoprotein biogenesis in the ER; relocalizes from ER to cytoplasm upon DNA replication stress
S000002630	YDR222W	""	""	910054	911301	1	Protein of unknown function	Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YDR222W has a paralog, YLR225C, that arose from the whole genome duplication
S000002631	YDR223W	CRF1	Co-Repressor with FHL1	912099	913502	1	Transcriptional corepressor	Transcriptional corepressor; involved in repression of ribosomal protein (RP) gene transcription via the TOR signaling pathway which promotes accumulation of Crf1p in the nucleus; role in repression of RP genes varies by strain; CRF1 has a paralog, IFH1, that arose from the whole genome duplication
S000002632	YDR224C	HTB1	Histone h Two B	914317	914712	-1	Histone H2B	Histone H2B; core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB2; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates reassembly after DNA replication, transcriptional activation, meiotic DSB formation and H3 methylation
S000002633	YDR225W	HTA1	Histone h Two A	915530	915928	1	Histone H2A	Histone H2A; core histone protein required for chromatin assembly and chromosome function; one of two nearly identical subtypes (see also HTA2); DNA damage-dependent phosphorylation by Mec1p facilitates DNA repair; acetylated by Nat4p; N-terminally propionylated in vivo
S000002634	YDR226W	ADK1	ADenylate Kinase	916486	917154	1	Adenylate kinase, required for purine metabolism	Adenylate kinase, required for purine metabolism; controls ATP homeostasis; localized to the cytoplasm and the mitochondria; lacks cleavable signal sequence; protein abundance increases in response to DNA replication stress; mutations affecting Adk1p catalytic activity deregulate expression of phosphate utilization genes PHO5 and PHO84; human homolog AK1 can complement yeast adk1 mutant
S000002635	YDR227W	SIR4	Silent Information Regulator	917571	921647	1	SIR protein involved in assembly of silent chromatin domains	SIR protein involved in assembly of silent chromatin domains; silent information regulator (SIR) along with SIR2 and SIR3; involved in assembly of silent chromatin domains at telomeres and the silent mating-type loci; some SIR4 alleles prolong lifespan; required for Ku-mediated telomerase recruitment, telomere lengthening, and telomere hypercluster formation in quiescent yeast cells
S000002636	YDR228C	PCF11	Protein 1 of Cleavage and polyadenylation Factor I	921926	923806	-1	mRNA 3' end processing factor	mRNA 3' end processing factor; essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping; relocalizes to the cytosol in response to hypoxia
S000002637	YDR229W	IVY1	Interacting with Vps33p and Ypt7p	924785	926146	1	Phospholipid-binding protein that interacts with both Ypt7p and Vps33p	Phospholipid-binding protein that interacts with both Ypt7p and Vps33p; may partially counteract the action of Vps33p and vice versa, localizes to the rim of the vacuole as cells approach stationary phase; contains a putative inverse BAR (I-BAR) domain that can mold lipid bilayer membranes into protrusions
S000002638	YDR230W	""	""	926223	926570	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20/YDR231C
S000002639	YDR231C	COX20	Cytochrome c OXidase	926293	926910	-1	Mitochondrial inner membrane protein	Mitochondrial inner membrane protein; required for proteolytic processing of Cox2p and its assembly into cytochrome c oxidase
S000002640	YDR232W	HEM1	HEMe biosynthesis	927452	929098	1	5-aminolevulinate synthase	5-aminolevulinate synthase; catalyzes the first step in the heme biosynthetic pathway; an N-terminal signal sequence is required for localization to the mitochondrial matrix; expression is regulated by Hap2p-Hap3p; has a pyridoxal phosphate cofactor whose insertion is mediated by Mcx1p
S000002641	YDR233C	RTN1	ReTiculoN-like	929470	930357	-1	Reticulon protein	Reticulon protein; involved in nuclear pore assembly and maintenance of tubular ER morphology; promotes membrane curvature; regulates the ER asymmetry-induced inheritance block during ER stress; role in ER-derived peroxisomal biogenesis; increases tubular ER when overexpressed; mutants have reduced phosphatidylserine transfer between the ER and mitochondria; interacts with exocyst subunit Sec6p, Yip3p, and Sbh1p; member of the RTNLA subfamily
S000002642	YDR234W	LYS4	LYSine requiring	931129	933210	1	Homoaconitase	Homoaconitase; catalyzes the conversion of homocitrate to homoisocitrate, which is a step in the lysine biosynthesis pathway
S000002643	YDR235W	PRP42	Pre-mRNA Processing	933504	935138	1	U1 snRNP protein involved in splicing	U1 snRNP protein involved in splicing; required for U1 snRNP biogenesis; contains multiple tetriatricopeptide repeats
S000002645	YDR237W	MRPL7	Mitochondrial Ribosomal Protein, Large subunit	936615	937493	1	Mitochondrial ribosomal protein of the large subunit	Mitochondrial ribosomal protein of the large subunit; MRPL7 produces both YmL5 and YmL7, which are two different modified forms of the same protein
S000002646	YDR238C	SEC26	SECretory	937895	940816	-1	Essential beta-coat protein of the COPI coatomer	Essential beta-coat protein of the COPI coatomer; involved in ER-to-Golgi protein trafficking and maintenance of normal ER morphology; shares 43% sequence identity with mammalian beta-coat protein (beta-COP)
S000002647	YDR239C	""	""	941057	943420	-1	Protein of unknown function	Protein of unknown function; may interact with ribosomes, based on co-purification experiments
S000002648	YDR240C	SNU56	Small NUclear ribonucleoprotein associated	943674	945152	-1	Component of U1 snRNP required for mRNA splicing via spliceosome	Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart; interacts with mRNA in commitment complex
S000002649	YDR241W	BUD26	BUD site selection	945151	945438	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 1% of ORF overlaps the verified gene SNU56; diploid mutant displays a weak budding pattern phenotype in a systematic assay
S000002650	YDR242W	AMD2	AMiDase	946807	948456	1	Putative amidase	Putative amidase
S000002651	YDR243C	PRP28	Pre-mRNA Processing	948518	950284	-1	Pre-mRNA splicing factor	Pre-mRNA splicing factor; RNA binding protein involved in RNA isomerization at the 5' splice site and for exchange of U6 for U1 snRNA at the 5' splice site; similar to the RNA helicases of the DEAD-box family
S000002652	YDR244W	PEX5	PEroXin	950563	952401	1	Peroxisomal membrane signal receptor for peroxisomal matrix proteins	Peroxisomal membrane signal receptor for peroxisomal matrix proteins; receptor for the C-terminal tripeptide signal sequence (PTS1) of peroxisomal matrix proteins; required for peroxisomal matrix protein import; also proposed to have PTS1-receptor independent functions
S000002653	YDR245W	MNN10	MaNNosyltransferase	952800	953981	1	Subunit of a Golgi mannosyltransferase complex	Subunit of a Golgi mannosyltransferase complex; complex mediates elongation of the polysaccharide mannan backbone; membrane protein of the mannosyltransferase family; other members of the complex are Anp1p, Mnn9p, Mnn11p, and Hoc1p
S000002654	YDR246W	TRS23	TRapp Subunit	954288	954947	1	Core component of transport protein particle (TRAPP) complexes I-III	Core component of transport protein particle (TRAPP) complexes I-III; TRAPP complexes are related multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating ER-Golgi traffic (TRAPPI), intra-Golgi traffic (TRAPPII), endosome-Golgi traffic (TRAPPII and III) and autophagy (TRAPPIII); human homolog is TRAPPC4
S000002655	YDR247W	VHS1	Viable in a Hal3 Sit4 background	956013	957398	1	Cytoplasmic serine/threonine protein kinase	Cytoplasmic serine/threonine protein kinase; identified as a high-copy suppressor of the synthetic lethality of a sis2 sit4 double mutant, suggesting a role in G1/S phase progression; VHS1 has a paralog, SKS1, that arose from the whole genome duplication
S000002656	YDR248C	""	""	957758	958339	-1	Putative gluconokinase	Putative gluconokinase; sequence similarity to bacterial and human gluconokinase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; upregulated by deletion of the RNAP-II associated factor, PAF1
S000002657	YDR249C	""	""	958681	959802	-1	Putative protein of unknown function	Putative protein of unknown function
S000002658	YDR250C	""	""	960086	960361	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000002659	YDR251W	PAM1	Pp2A Multicopy suppressor	960614	963106	1	Essential protein of unknown function	Essential protein of unknown function; exhibits variable expression during colony morphogenesis; overexpression permits survival without protein phosphatase 2A, inhibits growth, and induces a filamentous phenotype; PAM1 has a paralog, SVL3, that arose from the whole genome duplication
S000002660	YDR252W	BTT1	BTf Three	963412	963861	1	Heterotrimeric nascent polypeptide-associated complex beta3 subunit	Heterotrimeric nascent polypeptide-associated complex beta3 subunit; complex binds ribosomes via its beta-subunits in close proximity to nascent polypeptides; interacts with Caf130p of the CCR4-NOT complex; similar to human BTF3; BTT1 has a paralog, EGD1, that arose from the whole genome duplication
S000002661	YDR253C	MET32	METhionine requiring	963990	964565	-1	Zinc-finger DNA-binding transcription factor	Zinc-finger DNA-binding transcription factor; involved in transcriptional regulation of the methionine biosynthetic genes; targets strong transcriptional activator Met4p to promoters of sulfur metabolic genes; feedforward loop exists in the regulation of genes controlled by Met4p and Met32p; lack of such a loop for MET31 may account for the differential actions of Met32p and Met31p; MET32 has a paralog, MET31, that arose from the whole genome duplication
S000002662	YDR254W	CHL4	CHromosome Loss	965113	966489	1	Outer kinetochore protein required for chromosome stability	Outer kinetochore protein required for chromosome stability; involved in new kinetochore assembly and sister chromatid cohesion; forms a stable complex with Iml3p; peripheral component of the Ctf19 kinetochore complex that interacts with Ctf19p, Ctf3p, and Mif2p; required for the spindle assembly checkpoint; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-N and fission yeast mis15
S000002663	YDR255C	RMD5	Required for Meiotic nuclear Division	966557	967822	-1	Component of GID Complex that confers ubiquitin ligase (U3) activity	Component of GID Complex that confers ubiquitin ligase (U3) activity; necessary for polyubiquitination and degradation of the gluconeogenic enzyme fructose-1,6-bisphosphatase; forms dimer with Fyv10p that is then recruited to GID Complex by Gid8p; also required for sporulation; conserved protein that has a degenerate RING finger domain
S000002664	YDR256C	CTA1	CaTalase A	968133	969680	-1	Catalase A	Catalase A; breaks down hydrogen peroxide in the peroxisomal matrix formed by acyl-CoA oxidase (Pox1p) during fatty acid beta-oxidation
S000002665	YDR257C	RKM4	Ribosomal lysine (K) Methyltransferase	969990	971474	-1	Ribosomal lysine methyltransferase	Ribosomal lysine methyltransferase; specific for monomethylation of Rpl42ap and Rpl42bp (lysine 55); nuclear SET-domain containing protein
S000002666	YDR258C	HSP78	Heat Shock Protein	971808	974243	-1	Oligomeric mitochondrial matrix chaperone	Oligomeric mitochondrial matrix chaperone; cooperates with Ssc1p in mitochondrial thermotolerance after heat shock; prevents the aggregation of misfolded proteins as well as resolubilize protein aggregates; involved in response to ethanol stress
S000002667	YDR259C	YAP6	Yeast homolog of AP-1	974631	975782	-1	Basic leucine zipper (bZIP) transcription factor	Basic leucine zipper (bZIP) transcription factor; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; overexpression increases sodium and lithium tolerance; computational analysis suggests a role in regulation of expression of genes involved in carbohydrate metabolism; YAP6 has a paralog, CIN5, that arose from the whole genome duplication
S000002668	YDR260C	SWM1	Spore Wall Maturation	976717	977229	-1	Subunit of the anaphase-promoting complex (APC)	Subunit of the anaphase-promoting complex (APC); APC is an E3 ubiquitin ligase that regulates the metaphase-anaphase transition and exit from mitosis; required for activation of the daughter-specific gene expression and spore wall maturation
S000002669	YDR261C	EXG2	EXo-1,3-beta-Glucanase	977521	979209	-1	Exo-1,3-beta-glucanase	Exo-1,3-beta-glucanase; involved in cell wall beta-glucan assembly; may be anchored to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor
S000002670	YDR262W	""	""	993134	993952	1	Putative protein of unknown function	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole and is induced in response to the DNA-damaging agent MMS; gene expression increases in response to Zymoliase treatment
S000002671	YDR263C	DIN7	DNA Damage INducible	994242	995534	-1	Mitochondrial nuclease functioning in DNA repair and replication	Mitochondrial nuclease functioning in DNA repair and replication; modulates the stability of the mitochondrial genome, induced by exposure to mutagens, also induced during meiosis at a time nearly coincident with commitment to recombination; DIN7 has a paralog, EXO1, that arose from the whole genome duplication
S000002672	YDR264C	AKR1	AnKyrin Repeat containing	996029	998323	-1	Palmitoyl transferase involved in protein palmitoylation	Palmitoyl transferase involved in protein palmitoylation; acts as a negative regulator of pheromone response pathway; required for endocytosis of pheromone receptors; involved in cell shape control; contains ankyrin repeats; AKR1 has a paralog, AKR2, that arose from the whole genome duplication; any of several human homologs encoding DHHC-type zinc fingers (ZDHHC) can complement temperature sensitivity of yeast akr1 null mutant
S000002673	YDR265W	PEX10	PEroXin	998864	999877	1	Peroxisomal membrane E3 ubiquitin ligase	Peroxisomal membrane E3 ubiquitin ligase; required for for Ubc4p-dependent Pex5p ubiquitination and peroxisomal matrix protein import; contains zinc-binding RING domain; mutations in human homolog cause various peroxisomal disorders
S000002674	YDR266C	HEL2	Histone E3 Ligase	1000104	1002023	-1	RING finger ubiquitin ligase (E3)	RING finger ubiquitin ligase (E3); subunit of ribosome-associated quality control trigger complex (RQT); involved in ubiquitination and degradation of excess histones; interacts with Ubc4p and Rad53p; required to trigger ribosome-associated quality control (RQC) and canonical no-go decay; ubiquitinates Rps20 and Rps3; binds to ribosomal RNA, mRNA and tRNA
S000002675	YDR267C	CIA1	Cytosolic Iron-sulfur protein Assembly	1002510	1003502	-1	Component of cytosolic iron-sulfur protein assembly (CIA) machinery	Component of cytosolic iron-sulfur protein assembly (CIA) machinery; acts at late step of Fe-S cluster assembly; forms CIA targeting complex with Cia2p and Met18p that directs Fe-S cluster incorporation and maturation of a subset of cytosolic and nuclear proteins involved in methionine biosynthesis, DNA replication and repair, transcription and telomere maintenance; contains WD40 repeats; human homolog CIAO1 complements the yeast cia1 null mutant
S000002676	YDR268W	MSW1	Mitochondrial aminoacyl-tRNA Synthetase, tryptophan (W)	1004004	1005143	1	Mitochondrial tryptophanyl-tRNA synthetase	Mitochondrial tryptophanyl-tRNA synthetase
S000002677	YDR269C	""	""	1005665	1005988	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000002678	YDR270W	CCC2	Cross-Complements Ca(2+) phenotype of csg1	1005675	1008689	1	Cu(+2)-transporting P-type ATPase	Cu(+2)-transporting P-type ATPase; required for export of copper from cytosol into extracytosolic compartment; targeted to vacuole via AP-3 pathway; similar to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism; human homologs ATP7A and ATP7B both complement yeast null mutant
S000002679	YDR271C	""	""	1008395	1008766	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CCC2/YDR270W
S000002680	YDR272W	GLO2	GLyOxalase	1009010	1009834	1	Cytoplasmic glyoxalase II	Cytoplasmic glyoxalase II; catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate; GLO2 has a paralog, GLO4, that arose from the whole genome duplication
S000002681	YDR273W	DON1	DONut	1010176	1011273	1	Meiosis-specific component of the spindle pole body	Meiosis-specific component of the spindle pole body; subunit of the leading edge protein (LEP) complex (Ssp1-Ady3-Don1-Irc10) that forms a ring-like structure at the leading edge of the prospore membrane (PSM) during meiosis II; required for PSM growth and closure; DON1 has a paralog, CUE5, that arose from the whole genome
S000002682	YDR274C	""	""	1011589	1011960	-1	Putative protein of unknown function	Putative protein of unknown function; conserved among S. cerevisiae strains; YDR274C is not an essential gene
S000002683	YDR275W	BSC2	Bypass of Stop Codon	1012252	1012959	1	Protein of unknown function involved in multidrug resistance	Protein of unknown function involved in multidrug resistance; inhibits oxidative damage induced by amphotericin B; overexpression increases flocculation, biofilm formation, invasive and pseudohyphal growth; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; null mutant displays increased translation rate and increased readthrough of premature stop codons; BSC2 has a paralog, IRC23, that arose from the whole genome duplication
S000002684	YDR276C	PMP3	Plasma Membrane Proteolipid	1013476	1013643	-1	Small plasma membrane protein	Small plasma membrane protein; confers resistance to amphotericin B and is a potential target of this common antifungal drug; related to a family of plant polypeptides that are overexpressed under high salt concentration or low temperature; not essential for viability; deletion causes hyperpolarization of the plasma membrane potential
S000002685	YDR277C	MTH1	MSN Three Homolog	1014401	1015702	-1	Negative regulator of the glucose-sensing signal transduction pathway	Negative regulator of the glucose-sensing signal transduction pathway; required for repression of transcription by Rgt1p; interacts with Rgt1p and the Snf3p and Rgt2p glucose sensors; phosphorylated by Yck1p, triggering Mth1p degradation; MTH1 has a paralog, STD1, that arose from the whole genome duplication
S000002686	YDR278C	""	""	1017001	1017318	-1	Putative protein of unknown function	Putative protein of unknown function; conserved among S. cerevisiae strains; YDR278C is not an essential gene; overlaps tRNA-Glu/YNCD0021C
S000002687	YDR279W	RNH202	RNase H	1019368	1020420	1	Ribonuclease H2 subunit	Ribonuclease H2 subunit; required for RNase H2 activity; role in ribonucleotide excision repair; related to human AGS2 that causes Aicardi-Goutieres syndrome
S000002689	YDR281C	PHM6	PHosphate Metabolism	1022007	1022321	-1	Protein of unknown function	Protein of unknown function; expression is regulated by phosphate levels
S000002690	YDR282C	MRX10	Mitochondrial oRganization of gene eXpression (MIOREX)	1023511	1024755	-1	Mitochondrial inner membrane protein of unknown function	Mitochondrial inner membrane protein of unknown function; associates with mitochondrial ribosome; localizes to the inner membrane with the C terminus facing the intermembrane space; ortholog of human RMND1, mutation in which is implicated in infantile encephaloneuromyopathy and defective mitochondrial translation
S000002691	YDR283C	GCN2	General Control Nonderepressible	1025070	1030049	-1	Protein kinase	Protein kinase; phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control
S000002692	YDR284C	DPP1	Diacylglycerol Pyrophosphate Phosphatase	1030550	1031419	-1	Diacylglycerol pyrophosphate (DGPP) phosphatase	Diacylglycerol pyrophosphate (DGPP) phosphatase; zinc-regulated vacuolar membrane-associated lipid phosphatase, dephosphorylates DGPP to phosphatidate (PA) and Pi, then PA to diacylglycerol; involved in lipid signaling and cell metabolism
S000002693	YDR285W	ZIP1	molecular ZIPper	1032436	1035063	1	Transverse filament protein of the synaptonemal complex	Transverse filament protein of the synaptonemal complex; required for normal levels of meiotic recombination and pairing between homologous chromosome during meiosis; required for meiotic recombination between non-allelc sites; potential Cdc28p substrate
S000002694	YDR286C	MGP12	Mitochondrial Glutaredoxin-like Protein of 12 kDa	1035231	1035575	-1	Mitochondrial protein of unknown function	Mitochondrial protein of unknown function; predicted to have thiol-disulfide oxidoreductase active site
S000002695	YDR287W	INM2	INositol Monophosphatase	1035995	1036873	1	Inositol monophosphatase	Inositol monophosphatase; involved in biosynthesis of inositol; enzymatic activity requires magnesium ions and is inhibited by lithium and sodium ions; inm1 inm2 double mutant lacks inositol auxotrophy
S000002696	YDR288W	NSE3	Non SMC Element	1037195	1038106	1	Component of the SMC5-SMC6 complex	Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; protein abundance increases in response to DNA replication stress
S000002697	YDR289C	RTT103	Regulator of Ty1 Transposition	1038280	1039509	-1	Protein implicated in transcription termination by RNA polymerase II	Protein implicated in transcription termination by RNA polymerase II; binds to the RNA polymerase II C-terminal domain (CTD) phosphorylated on serine (ser2); interacts with 5' to 3' exonuclease Rat1p and decapping endonuclease Rai1p and is involved in mRNA 3' end processing; contains an RPR domain (CTD-interacting domain); involved in regulation of Ty1 transposition
S000002698	YDR290W	""	""	1039370	1039699	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RTT103
S000002699	YDR291W	HRQ1	Homologous to RecQ protein	1039728	1042961	1	3'-5' DNA helicase of the conserved RecQ family	3'-5' DNA helicase of the conserved RecQ family; involved in Pso2p-mediated interstrand crosslink repair; acts with Rad4p in nucleotide-excision repair; binds telomeres, modulates telomerase activity with Pif1p and inhibits telomere addition to dsDNA breaks; role in telomerase-independent telomere maintenance; lacks ssDNA annealing and strand exchange activities; human RecQL4, a structural and functional homolog, is involved in Rothmund-Thomson, Baller-Gerold and RAPADILINO syndromes
S000002700	YDR292C	SRP101	Signal Recognition Particle	1043146	1045011	-1	Signal recognition particle (SRP) receptor alpha subunit	Signal recognition particle (SRP) receptor alpha subunit; contain GTPase domains; involved in SRP-dependent protein targeting; interacts with the beta subunit, Srp102p
S000002701	YDR293C	SSD1	Suppressor of SIT4 Deletion	1045640	1049392	-1	Translational repressor	Translational repressor;  involved in polar growth, wall integrity, autodiploidization; regulated by Cbk1p phosphorylation to affect bud-specific translational control and localization of specific mRNAs; binds 5'UTRs of mRNAs encoding cell wall proteins; recognizes CNYTCNYT motif in RNA; interacts with TOR pathway components; nucleocytoplasmic shuttling appears to be critical to Ssd1p function; W303 lacks a functional SSD1 gene product; pseudonuclease descended from Dis3L2-family nucleases
S000002702	YDR294C	DPL1	Dihydrosphingosine Phosphate Lyase	1050459	1052228	-1	Dihydrosphingosine phosphate lyase	Dihydrosphingosine phosphate lyase; regulates intracellular levels of sphingolipid long-chain base phosphates (LCBPs), degrades phosphorylated long chain bases, prefers C16 dihydrosphingosine-l-phosphate as a substrate; localizes to the ER and to the peroxisome
S000002703	YDR295C	HDA2	Histone DeAcetylase	1052623	1054647	-1	Subunit of HDA1 histone deacetylase complex	Subunit of HDA1 histone deacetylase complex; tetrameric trichostatin A-sensitive class II histone deacetylase complex contains Hda1p homodimer and an Hda2p-Hda3p heterodimer; involved in telomere maintenance; Hda2p relocalizes to cytosol in response to hypoxia; other members of the HDA1 histone deacetylase complex, which regulates aging through trehalose metabolism, are Hda1p and Hda3p
S000002704	YDR296W	MHR1	Mitochondrial Homologous Recombination	1055212	1055892	1	Mitochondrial ribosomal protein of the large subunit	Mitochondrial ribosomal protein of the large subunit; also involved in homologous recombination in mitochondria; required for recombination-dependent mtDNA partitioning; involved in stimulation of mitochondrial DNA replication in response to oxidative stress
S000002705	YDR297W	SUR2	SUppressor of Rvs161 and rvs167 mutations	1056551	1057600	1	Sphinganine C4-hydroxylase	Sphinganine C4-hydroxylase; catalyses the conversion of sphinganine to phytosphingosine in sphingolipid biosyntheis
S000002706	YDR298C	ATP5	ATP synthase	1058176	1058814	-1	Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase	Subunit 5 of the stator stalk of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; homologous to bovine subunit OSCP (oligomycin sensitivity-conferring protein); phosphorylated
S000002707	YDR299W	BFR2	BreFeldin A Resistance	1059627	1061231	1	Component of the SSU and 90S preribosomes	Component of the SSU and 90S preribosomes; involved in pre-18S rRNA processing; binds to U3 snoRNA and Mpp10p; multicopy suppressor of sensitivity to Brefeldin A; expression is induced during lag phase and also by cold shock
S000002708	YDR300C	PRO1	PROline requiring	1061505	1062791	-1	Gamma-glutamyl kinase	Gamma-glutamyl kinase; catalyzes the first step in proline biosynthesis; required for nitrogen starvation-induced ribophagy but not for nonselective autophagy; PRO1 has a paralog, YHR033W, that arose from the whole genome duplication
S000002709	YDR301W	CFT1	Cleavage Factor Two	1063352	1067425	1	RNA-binding subunit of the mRNA cleavage and polyadenylation factor	RNA-binding subunit of the mRNA cleavage and polyadenylation factor; involved in poly(A) site recognition and required for both pre-mRNA cleavage and polyadenylation, 51% sequence similarity with mammalian AAUAA-binding subunit of CPSF
S000002710	YDR302W	GPI11	GlycosylPhosphatidylInositol anchor biosynthesis	1067731	1068390	1	ER membrane protein involved in a late step of GPI anchor assembly	ER membrane protein involved in a late step of GPI anchor assembly; involved in the addition of phosphoethanolamine to the multiply mannosylated glycosylphosphatidylinositol (GPI) intermediate; human PIG-Fp is a functional homolog
S000002711	YDR303C	RSC3	Remodel the Structure of Chromatin	1068729	1071386	-1	Component of the RSC chromatin remodeling complex	Component of the RSC chromatin remodeling complex; essential gene required for maintenance of proper ploidy and regulation of ribosomal protein genes and the cell wall/stress response; RSC3 has a paralog, RSC30, that arose from the whole genome duplication
S000002712	YDR304C	CPR5	Cyclosporin-sensitive Proline Rotamase	1071880	1072557	-1	Peptidyl-prolyl cis-trans isomerase (cyclophilin) of the ER	Peptidyl-prolyl cis-trans isomerase (cyclophilin) of the ER; catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; transcriptionally induced in response to unfolded proteins in the ER; CPR5 has a paralog, CPR2, that arose from the whole genome duplication
S000002713	YDR305C	HNT2	Histidine triad NucleoTide-binding	1072746	1073488	-1	Dinucleoside triphosphate hydrolase	Dinucleoside triphosphate hydrolase; has similarity to the tumor suppressor FHIT and belongs to the histidine triad (HIT) superfamily of nucleotide-binding proteins
S000002714	YDR306C	PFU1	Pheromone, F-box, Ubiquitination	1073735	1075171	-1	F-box protein that regulates pheromone-dependent morphogenesis	F-box protein that regulates pheromone-dependent morphogenesis; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain
S000002715	YDR307W	PMT7	""	1075865	1077853	1	Putative protein mannosyltransferase similar to Pmt1p	Putative protein mannosyltransferase similar to Pmt1p; has a potential role in protein O-glycosylation
S000002716	YDR308C	SRB7	Suppressor of RNA polymerase B	1078027	1078449	-1	Subunit of the RNA polymerase II mediator complex	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; target of the global repressor Tup1p
S000002717	YDR309C	GIC2	GTPase Interactive Component	1079048	1080199	-1	Rho-family GTPase interacting protein and Cdc42p effector	Rho-family GTPase interacting protein and Cdc42p effector; required with Gic1p for cell polarity establishment and initiation of budding; involved in recruitment of septins to the presumptive bud site; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain and with PI(4,5)P2 via a polybasic region; gic1 gic2 double mutants are defective for polarizing Cdc42p at elevated temperature, so may function both up- and downstream of Cdc42p; regulates mitotic exit
S000002718	YDR310C	SUM1	SUppresor of Mar1-1	1081128	1084316	-1	Transcriptional repressor that regulates middle-sporulation genes	Transcriptional repressor that regulates middle-sporulation genes; required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint
S000002719	YDR311W	TFB1	Transcription Factor B	1085065	1086993	1	Subunit of TFIIH and nucleotide excision repair factor 3 complexes	Subunit of TFIIH and nucleotide excision repair factor 3 complexes; required for nucleotide excision repair, target for transcriptional activators; relocalizes to the cytosol in response to hypoxia
S000002720	YDR312W	SSF2	Suppressor of ste4 (Four)	1087581	1088942	1	Protein required for ribosomal large subunit maturation	Protein required for ribosomal large subunit maturation; functionally redundant with Ssf1p; member of the Brix family; SSF2 has a paralog, SSF1, that arose from the whole genome duplication
S000002721	YDR313C	PIB1	PtdIns(3)p-Binding	1089219	1090079	-1	RING-type ubiquitin ligase of the endosomal and vacuolar membranes	RING-type ubiquitin ligase of the endosomal and vacuolar membranes; binds phosphatidylinositol 3-phosphate; contains a FYVE finger domain
S000002722	YDR314C	RAD34	Homologous to RAD4	1090433	1092511	-1	Protein involved in nucleotide excision repair (NER)	Protein involved in nucleotide excision repair (NER); homologous to RAD4
S000002723	YDR315C	IPK1	Inositol Polyphosphate Kinase	1092741	1093586	-1	Inositol 1,3,4,5,6-pentakisphosphate 2-kinase	Inositol 1,3,4,5,6-pentakisphosphate 2-kinase; nuclear protein required for synthesis of 1,2,3,4,5,6-hexakisphosphate (phytate), which is integral to cell function; has 2 motifs conserved in other fungi; ipk1 gle1 double mutant is inviable; human IPPK can complement ipk1 null mutant
S000002724	YDR316W	OMS1	OXA1 Multicopy Suppressor	1093763	1095178	1	Protein integral to the mitochondrial membrane	Protein integral to the mitochondrial membrane; has a conserved methyltransferase motif and is predicted to be an RNA methyltransferase; multicopy suppressor of respiratory defects caused by OXA1 mutations
S000002725	YDR317W	HIM1	High Induced Mutagenesis	1102184	1103428	1	Protein of unknown function involved in DNA repair	Protein of unknown function involved in DNA repair
S000002726	YDR318W	MCM21	MiniChromosome Maintenance	1103758	1104947	1	Component of the kinetochore sub-complex COMA	Component of the kinetochore sub-complex COMA; COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) bridges kinetochore subunits in contact with centromeric DNA with subunits bound to microtubules during kinetochore assembly; involved in minichromosome maintenance; modified by sumoylation; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-O
S000002727	YDR319C	YFT2	""	1105003	1105827	-1	Protein needed for ER membrane biosynthesis in response to ER stress	Protein needed for ER membrane biosynthesis in response to ER stress; member of the highly conserved FIT family of proteins involved in triglyceride droplet biosynthesis; homologous to human FIT2/FITM2, a fatty acyl-CoA diphosphatase; interacts with Sst2p and Hsp82p in high-throughput two-hybrid screens 
S000002728	YDR320C	SWA2	Synthetic lethal With Arf1	1106095	1108101	-1	Auxilin-like protein involved in vesicular transport	Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles; required for propagation of [URE3] prion variant
S000002729	YDR321W	ASP1	ASParaginase	1108702	1109847	1	Cytosolic L-asparaginase, involved in asparagine catabolism	Cytosolic L-asparaginase, involved in asparagine catabolism; catalyzes hydrolysis of L-asparagine to aspartic acid and ammonia; important enzyme for the treatment of acute lymphoblastic leukemia; has low glutaminase activity and dependence; synthesized constitutively
S000002730	YDR322W	MRPL35	Mitochondrial Ribosomal Protein, Large subunit	1110589	1111692	1	Mitochondrial ribosomal protein of the large subunit	Mitochondrial ribosomal protein of the large subunit; involved together with Mrp7p in assembly of cytochrome c oxidase
S000002731	YDR323C	PEP7	carboxyPEPtidase Y-deficient	1112480	1114027	-1	Adaptor protein involved in vesicle-mediated vacuolar protein sorting	Adaptor protein involved in vesicle-mediated vacuolar protein sorting; multivalent adaptor protein; facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance
S000002732	YDR324C	UTP4	U Three Protein	1114433	1116763	-1	Subunit of U3-containing 90S preribosome and SSU processome complexes	Subunit of U3-containing 90S preribosome and SSU processome complexes; involved in production of 18S rRNA and assembly of small ribosomal subunit; member of t-Utp subcomplex involved with transcription of 35S rRNA transcript; Small Subunit processome is also known as SSU processome
S000002733	YDR325W	YCG1	Yeast Cap G	1117126	1120233	1	Subunit of the condensin complex	Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation and chromatin binding by the complex; required for tRNA genes clustering at the nucleolus; required for replication slow zone breakage following Mec1p inactivation; transcription is cell cycle regulated, peaking in mitosis and declining in G1; protein is constitutively degraded by the proteasome; rate limiting for condensin recruitment to chromatin
S000002734	YDR326C	YSP2	Yeast Suicide Protein	1120609	1124925	-1	Sterol-binding protein	Sterol-binding protein; has a probable role in retrograde transport of sterols from the plasma membrane to the ER; contains two StART-like domains that bind sterols and a GRAM domain; co-localizes to puncta in the cortical ER with Sip3p; one of six StART-like domain-containing proteins in yeast that may be involved in sterol transfer between intracellular membranes; conserved across eukaryotes
S000002737	YDR329C	PEX3	PEroXin	1126270	1127595	-1	Peroxisomal membrane protein (PMP)	Peroxisomal membrane protein (PMP); required for proper localization and stability of PMPs; anchors peroxisome retention factor Inp1p at the peroxisomal membrane; interacts with Pex19p
S000002738	YDR330W	UBX5	UBiquitin regulatory X	1127872	1129374	1	UBX domain-containing protein that interacts with Cdc48p	UBX domain-containing protein that interacts with Cdc48p; ubiquitin regulatory X is also known as UBX
S000002739	YDR331W	GPI8	GlycosylPhosphatidylInositol anchor biosynthesis	1129588	1130823	1	Catalytic subunit of the ER membrane GPI transamidase complex	Catalytic subunit of the ER membrane GPI transamidase complex; involved in adding glycosylphosphatidylinositol (GPI) anchors to newly synthesized proteins; luminally-oriented type I integral membrane glycoprotein of the ER; human PIGK protein is a functional homolog
S000002740	YDR332W	IRC3	Increased Recombination Centers	1131001	1133070	1	Double-stranded DNA-dependent helicase of the DExH/D-box family	Double-stranded DNA-dependent helicase of the DExH/D-box family; branch point-binding helicase that preferentially unwinds the nascent lagging strand with a 3' to 5' polarity at a replication fork; contains double-stranded DNA translocase activity; responsible for DNA branch migration; required for maintenance of the mitochondrial (mt) genome; localizes to the mitochondrial matrix; monomeric
S000002741	YDR333C	RQC1	Ribosome Quality Control	1133260	1135431	-1	Component of the ribosome quality control complex (RQC)	Component of the ribosome quality control complex (RQC); RQC (Rqc1p-Rkr1p-Rqc2p-Cdc48p-Npl4p-Ufd1p) is a ribosome-bound complex required for the degradation of polypeptides arising from stalled translation; required along with Rkr1p for recruitment of the Cdc48p-Npl4p-Ufd1p AAA ATPase complex to the RQC
S000002742	YDR334W	SWR1	SWi2/snf2-Related	1135932	1140476	1	Swi2/Snf2-related ATPase	Swi2/Snf2-related ATPase; catalytic subunit of SWR1 complex, which exchanges histone variant H2A.Z (Htz1p) for chromatin-bound histone H2A; N-terminus can deliver H2A.Z-H2B dimer to DNA-(H3-H4)2 tetrasome; relocalizes to cytosol in response to hypoxia; chronological aging factor, mediates lifespan extension by dietary restriction
S000002743	YDR335W	MSN5	Multicopy suppressor of SNf1 mutation	1141168	1144842	1	Karyopherin	Karyopherin; involved in nuclear import and export of proteins, including import of replication protein A and export of Far1p and transcription factors Swi5p, Swi6p, Msn2p, and Pho4p; required for re-export of mature tRNAs after their retrograde import from the cytoplasm; exportin-5 homolog
S000002744	YDR336W	MRX8	Mitochondrial oRganization of gene eXpression (MIOREX)	1145092	1146036	1	Conserved mitochondrial YihA GTPase family member	Conserved mitochondrial YihA GTPase family member; required for efficient cellular respiration; localizes to mitochondrial inner membrane facing matrix side; associates with mitochondrial ribosome; required for de novo Cox1p synthesis at suboptimal temperatures; sumoylated under stress conditions
S000002745	YDR337W	MRPS28	Mitochondrial Ribosomal Protein, Small subunit	1146319	1147179	1	Mitochondrial ribosomal protein of the small subunit	Mitochondrial ribosomal protein of the small subunit
S000002746	YDR338C	""	""	1147379	1149466	-1	Putative protein of unknown function	Putative protein of unknown function; member of the multi-drug and toxin extrusion (MATE) family of the multidrug/oligosaccharidyl-lipid/polysaccharide (MOP) exporter superfamily
S000002747	YDR339C	FCF1	Faf1p Copurifying Factor	1149951	1150520	-1	PINc domain endonuclease	PINc domain endonuclease; required for early cleavage of 35S pre-rRNA and maturation of 18S rRNA; component of the SSU (small subunit) processome involved in 40S ribosomal subunit biogenesis; copurifies with Faf1p 
S000002748	YDR340W	""	""	1150881	1151183	1	Putative protein of unknown function	Putative protein of unknown function
S000002749	YDR341C	""	""	1151803	1153626	-1	Arginyl-tRNA synthetase	Arginyl-tRNA synthetase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDR341C has a paralog, MSR1, that arose from the whole genome duplication
S000002752	YDR344C	""	""	1162006	1162449	-1	Putative protein of unknown function	Putative protein of unknown function; conserved among S. cerevisiae strains
S000002753	YDR345C	HXT3	HeXose Transporter	1162957	1164660	-1	Low affinity glucose transporter of the major facilitator superfamily	Low affinity glucose transporter of the major facilitator superfamily; expression is induced in low or high glucose conditions; HXT3 has a paralog, HXT5, that arose from the whole genome duplication
S000002754	YDR346C	SVF1	SurVival Factor	1167214	1168659	-1	Ceramide binding protein	Ceramide binding protein; putative ceramide transfer protein involved in the nonvesicular transport of ceramides between the endoplasmic reticulum and Golgi apparatus; contributes to sphingolipid biosynthesis at the cis-Golgi; localizes to the cis-Golgi and the cytoplasm; required for the diauxic growth shift; promotes survival in mammalian cells under apoptosis-inducing conditions; mutant has increased aneuploidy tolerance                                                   
S000002755	YDR347W	MRP1	Mitochondrial Ribosomal Protein	1169178	1170143	1	Mitochondrial ribosomal protein of the small subunit	Mitochondrial ribosomal protein of the small subunit; MRP1 exhibits genetic interactions with PET122, encoding a COX3-specific translational activator, and with PET123, encoding a small subunit mitochondrial ribosomal protein
S000002756	YDR348C	PAL1	Pears And Lemons	1170326	1171825	-1	Protein of unknown function thought to be involved in endocytosis	Protein of unknown function thought to be involved in endocytosis; physically interacts with Ede1p and is found at endocytic sites at cell periphery during early stages of endocytosis; green fluorescent protein (GFP)-fusion protein localizes to bud neck; potential Cdc28p substrate; similar to S. pombe Pal1 protein; relocalizes from bud neck to cytoplasm upon DNA replication stress; PAL1 has a paralog, YHR097C, that arose from the whole genome duplication
S000002757	YDR349C	YPS7	YaPSin	1172386	1174176	-1	Putative GPI-anchored aspartic protease	Putative GPI-anchored aspartic protease; member of the yapsin family of proteases involved in cell wall growth and maintenance; located in the cytoplasm and endoplasmic reticulum
S000002758	YDR350C	ATP22	ATPase synthase	1176120	1178174	-1	Specific translational activator for the mitochondrial ATP6 mRNA	Specific translational activator for the mitochondrial ATP6 mRNA; Atp6p encodes a subunit of F1F0 ATP synthase; localized to the mitochondrial inner membrane
S000002759	YDR351W	SBE2	Suppressor of BEm4	1178666	1181260	1	Protein required for bud growth	Protein required for bud growth; involved in transport of cell wall components from the Golgi to the cell surface; SBE2 has a paralog, SBE22, that arose from the whole genome duplication
S000002760	YDR352W	YPQ2	Yeast PQ-loop protein	1181801	1182754	1	Putative vacuolar membrane transporter for cationic amino acids	Putative vacuolar membrane transporter for cationic amino acids; involved in vacuolar export of arginine under nitrogen starvation conditions; appears to be inactive under nitrogen replete conditions; may contribute to amino acid homeostasis; vacuolar membrane protein; member of the PQ-loop family, containing seven transmembrane domains; homologous to the human lysosomal cationic amino acid exporter PQLC2; null mutant is functionally complemented by the rat PQLC2 vacuolar transporter
S000002762	YDR354W	TRP4	TRyPtophan	1184747	1185889	1	Anthranilate phosphoribosyl transferase	Anthranilate phosphoribosyl transferase; transferase of the tryptophan biosynthetic pathway; catalyzes the phosphoribosylation of anthranilate; subject to the general control system of amino acid biosynthesis
S000002763	YDR355C	""	""	1186067	1186369	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified essential ORF SPC110/YDR356W
S000002764	YDR356W	SPC110	Spindle Pole Component	1186107	1188941	1	Inner plaque spindle pole body (SPB) component	Inner plaque spindle pole body (SPB) component; gamma-tubulin small complex (gamma-TuSC) receptor that interacts with Spc98p to recruit the complex to the nuclear side of the SPB, connecting nuclear microtubules to the SPB; promotes gamma-TuSC assembly and oligomerization to initiate microtubule nucleation; interacts with Tub4p-complex and calmodulin; phosphorylated by Mps1p in cell cycle-dependent manner; ortholog of human pericentrin (kendrin)
S000002765	YDR357C	CNL1	CNo-Like	1189199	1189567	-1	Subunit of the BLOC-1 complex involved in endosomal maturation	Subunit of the BLOC-1 complex involved in endosomal maturation; interacts with Msb3p; null mutant is sensitive to drug inducing secretion of vacuolar cargo; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
S000002766	YDR358W	GGA1	Golgi-localized, Gamma-adaptin ear homology, Arf-binding protein	1190059	1191732	1	Golgi-localized protein with homology to gamma-adaptin	Golgi-localized protein with homology to gamma-adaptin; interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi; GGA1 has a paralog, GGA2, that arose from the whole genome duplication
S000002767	YDR359C	EAF1	Esa1p-Associated Factor	1191936	1194884	-1	Component of the NuA4 histone acetyltransferase complex	Component of the NuA4 histone acetyltransferase complex; acts as a platform for assembly of NuA4 subunits into the native complex; required for initiation of pre-meiotic DNA replication, likely due to its requirement for expression of IME1
S000002768	YDR360W	OPI7	OverProducer of Inositol	1194598	1195032	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified gene VID21/YDR359C
S000002769	YDR361C	BCP1	""	1195412	1196263	-1	Essential nuclear chaperone for ribosomal protein Rpl23p	Essential nuclear chaperone for ribosomal protein Rpl23p; involved in nuclear export of Mss4p; Mss4p is a lipid kinase that generates phosphatidylinositol 4,5-biphosphate and plays a role in actin cytoskeleton organization and vesicular transport
S000002770	YDR362C	TFC6	Transcription Factor C	1196679	1198697	-1	Subunit of RNA polymerase III transcription initiation factor complex	Subunit of RNA polymerase III transcription initiation factor complex; one of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110
S000002771	YDR363W	ESC2	Establishment of Silent Chromatin	1199183	1200553	1	SUMO-like domain protein	SUMO-like domain protein; binds Holliday junctions and replication fork structures; stimulates the Mms4-Mus81 endonuclease in the resolution of sister chromatid junctions; role in recombination-mediated DNA damage tolerance by recruiting Rad51p; facilitate Elg1p binding to stalled replication forks; prevents toxic intermediate accumulation during replication-associated recombinational repair; role in silencing, chromatid cohesion and the intra-S-phase DNA damage checkpoint; RENi family member
S000002772	YDR364C	CDC40	Cell Division Cycle	1202843	1204210	-1	Pre-mRNA splicing factor	Pre-mRNA splicing factor; important for catalytic step II of pre-mRNA splicing and plays a role in cell cycle progression, particularly at the G1/S phase transition; required for DNA synthesis during mitosis and meiosis; has WD repeats; thermosensitivity of the cdc40 null mutant is functionally complemented by a chimeric construct containing the N-terminal 156 amino acids of yeast Cdc40p fused to the C-terminal two thirds (297 amino acids) of human CDC40
S000002775	YDR367W	KEI1	Kex2-cleavable protein Essential for Inositol phosphorylceramide synthesis	1212848	1213614	1	Component of inositol phosphorylceramide (IPC) synthase	Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene
S000002776	YDR368W	YPR1	Yeast Putative Reductase	1213904	1214842	1	NADPH-dependent aldo-keto reductase	NADPH-dependent aldo-keto reductase; reduces multiple substrates including 2-methylbutyraldehyde and D,L-glyceraldehyde, expression is induced by osmotic and oxidative stress; functionally redundant with other aldo-keto reductases; protein abundance increases in response to DNA replication stress; YPR1 has a paralog, GCY1, that arose from the whole genome duplication; human homolog AKR1B1 can complement yeast null mutant
S000002777	YDR369C	XRS2	X-Ray Sensitive	1215016	1217580	-1	FHA domain-containing component of the Mre11 complex	FHA domain-containing component of the Mre11 complex; MRE11-RAD50-XRS2 complex is involved in double strand breaks, meiotic recombination, telomere maintenance, and checkpoint signaling;  FHA domain is required for activation of Tel1p
S000002778	YDR370C	DXO1	Decapping eXOnuclease	1217782	1219110	-1	Cytoplasmic 5' exoribonuclease	Cytoplasmic 5' exoribonuclease; transcriptomics wide distributive exonuclease; involved in cytoplasmic mRNA degradation, turnover of tRNA introns and processing of spliceosomal introns; required for the final 5'-end processing of 25S rRNA; minor contributor to No-Go Decay and the unfolded protein response; decapping protein involved in surveillance and removal of non-canonical nicotinamide adenine dinucleotide (NAD) capped nuclear mRNAs (NAD-RNAs); sequence similarity to Rai1p 
S000002779	YDR371W	CTS2	ChiTinaSe	1219413	1220948	1	Chitinase	Chitinase; contains both exochitinase and endochitinase activities in vitro with higher chitobiosidase activity; glycoside hydrolase GH18 family member with putative N-terminal transmembrane domain; functionally complements A. gossypii cts2 mutant sporulation defect
S000002780	YDR372C	VPS74	Vacuolar Protein Sorting	1221112	1222149	-1	Golgi PI4K effector, PtdIns4P sensor, and retention receptor	Golgi PI4K effector, PtdIns4P sensor, and retention receptor; interacts with glycosyltransferases, and in the PtdIns4P-bound state mediates retention of these enzymes in the Golgi; PtdIns4P sensor that limits PI4K signaling and regulates lipid homeostasis, interacting with the catalytic domain of Sac1p, the major PtdIns4P phosphatase, directing dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; contributes to telomere function; ortholog of human GOLPH3
S000002781	YDR373W	FRQ1	FReQuenin homolog	1222759	1223331	1	N-myristoylated calcium-binding protein	N-myristoylated calcium-binding protein; may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily; human NCS1 functionally complements the heat sensitivity of a frq1 ts mutant
S000002782	YDR374C	PHO92	PHOsphate metabolism	1223474	1224394	-1	N6-Methyladenosine (m6A) reader	N6-Methyladenosine (m6A) reader; co-transcriptionally recruited to specific methylated mRNAs during meiotic prophase, contributing to the down-regulation of transcript abundance, and the timing of meiotic recombination; regulates PHO4 mRNA stability, binding to the 3'UTR in a phosphate-dependent manner; posttranscriptional regulator of phosphate and glucose metabolism; contains a highly conserved YTH domain that exhibits RNA-binding activity; human homolog YTHDF2 can complement yeast null mutant
S000002783	YDR375C	BCS1	ubiquinol-cytochrome c reductase (bc1) Synthesis	1225166	1226536	-1	Protein translocase and chaperone required for Complex III assembly	Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane and delivers it to an assembly intermediate of respiratory Complex III; also required for assembly of the Qcr10p subunit; mutation is functionally complemented by human homolog BCS1L, linked to neonatal diseases
S000002784	YDR376W	ARH1	Adrenodoxin Reductase Homolog	1226822	1228303	1	Oxidoreductase of the mitochondrial inner membrane	Oxidoreductase of the mitochondrial inner membrane; involved in cytoplasmic and mitochondrial iron homeostasis and required for activity of Fe-S cluster-containing enzymes; one of the few mitochondrial proteins essential for viability
S000002785	YDR377W	ATP17	ATP synthase	1228611	1228916	1	Subunit f of the F0 sector of mitochondrial F1F0 ATP synthase	Subunit f of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis
S000002786	YDR378C	LSM6	Like SM	1229349	1229609	-1	Lsm (Like Sm) protein	Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA
S000002787	YDR379W	RGA2	Rho GTPase Activating Protein	1230167	1233196	1	GTPase-activating protein for polarity-establishment protein Cdc42p	GTPase-activating protein for polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; regulated by Pho85p and Cdc28p; RGA2 has a paralog, RGA1, that arose from the whole genome duplication
S000002788	YDR380W	ARO10	AROmatic amino acid requiring	1234218	1236125	1	Phenylpyruvate decarboxylase	Phenylpyruvate decarboxylase; catalyzes decarboxylation of phenylpyruvate to phenylacetaldehyde, which is the first specific step in the Ehrlich pathway; involved in protein N-terminal Met and Ala catabolism
S000002789	YDR381W	YRA1	Yeast RNA Annealing protein	1236558	1238004	1	Nuclear polyadenylated RNA-binding protein	Nuclear polyadenylated RNA-binding protein; required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; interacts with DBP2; inhibits the helicase activity of Dbp2; functionally redundant with Yra2p, another REF family member
S000002790	YDR382W	RPP2B	Ribosomal Protein P2 Beta	1239492	1239824	1	Ribosomal protein P2 beta	Ribosomal protein P2 beta; a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; free (non-ribosomal) P2 stimulates the phosphorylation of the eIF2 alpha subunit (Sui2p) by Gcn2p; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm
S000002791	YDR383C	NKP1	Non-essential Kinetochore Protein	1239960	1240676	-1	Central kinetochore protein and subunit of the Ctf19 complex	Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein fta4
S000002792	YDR384C	ATO3	Ammonia (Ammonium) Transport Outward	1241204	1242031	-1	Plasma membrane protein, putative ammonium transporter	Plasma membrane protein, putative ammonium transporter; regulation pattern suggests a possible role in export of ammonia from the cell; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family of putative transporters
S000002793	YDR385W	EFT2	Elongation Factor Two	1243230	1245758	1	Eukaryotic translation elongation factor 2 (eEF2, EF-2)	Eukaryotic translation elongation factor 2 (eEF2, EF-2); also encoded by EFT1; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT2 has a paralog, EFT1, that arose from the whole genome duplication
S000002794	YDR386W	MUS81	MMS and UV Sensitive	1246084	1247982	1	Subunit of structure-specific Mms4p-Mus81p endonuclease	Subunit of structure-specific Mms4p-Mus81p endonuclease; cleaves branched DNA and DNA-protein cross-links; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; promotes template switching during break-induced replication (BIR), causing non-reciprocal translocations (NRTs); helix-hairpin-helix protein; phosphorylation of non-catalytic subunit Mms4p by Cdc28p and Cdc5p during mitotic cell cycle activates function of Mms4p-Mus81p
S000002795	YDR387C	CIN10	Chromosome INstability	1248154	1249821	-1	Putative transporter	Putative transporter; member of the sugar porter family; non-essential gene; overexpression results in elevated colony sectoring, an indicator of chromosomal instability
S000002796	YDR388W	RVS167	Reduced Viability on Starvation	1250186	1251634	1	Calmodulin-binding actin-associated protein	Calmodulin-binding actin-associated protein; roles in endocytic membrane tabulation and constriction, and exocytosis; N-BAR domain protein that interacts with Rvs161p to regulate actin cytoskeleton, endocytosis, and viability following starvation or osmotic stress; recruited to bud tips by Gyl1p and Gyp5p during polarized growth; homolog of mammalian amphiphysin; necessary for efficient trafficking of CoQ6 to mitochondria
S000002797	YDR389W	SAC7	Suppressor of ACtin	1252537	1254501	1	GTPase activating protein (GAP) for Rho1p	GTPase activating protein (GAP) for Rho1p; regulator of a Tor2p-mediated, Rho1p GTPase switch that controls organization of the actin cytoskeleton; negative regulator of the RHO1-PKC1-MAPK cell integrity (CWI) and membrane fluidity homeostasis signaling pathways; potential Cdc28p substrate; SAC7 has a paralog, BAG7, that arose from the whole genome duplication
S000002798	YDR390C	UBA2	UBiquitin Activating	1254937	1256847	-1	Subunit of heterodimeric nuclear SUMO activating enzyme E1 with Aos1p	Subunit of heterodimeric nuclear SUMO activating enzyme E1 with Aos1p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability
S000002799	YDR391C	""	""	1257358	1258056	-1	Putative protein of unknown function	Putative protein of unknown function; possibly involved in zinc homeostasis; Bdf1p-dependent transcription induced by salt stress; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
S000002800	YDR392W	SPT3	SuPpressor of Ty's	1258696	1259709	1	Subunit of the SAGA and SAGA-like transcriptional regulatory complexes	Subunit of the SAGA and SAGA-like transcriptional regulatory complexes; interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters; role in preventing L-A mycovirus pathogenesis; relocalizes to the cytosol in response to hypoxia
S000002801	YDR393W	SHE9	Sensitivity to High Expression	1259901	1261271	1	Protein required for normal mitochondrial morphology	Protein required for normal mitochondrial morphology; localized to mitochondrial inner membrane; may be involved in fission of the inner membrane; forms a homo-oligomeric complex
S000002802	YDR394W	RPT3	Regulatory Particle Triple-A protein, or Regulatory Particle Triphosphatase	1261681	1262967	1	ATPase of the 19S regulatory particle of the 26S proteasome	ATPase of the 19S regulatory particle of the 26S proteasome; one of ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; substrate of N-acetyltransferase B
S000002803	YDR395W	SXM1	Suppressor of mRNA eXport Mutant	1263324	1266158	1	Nuclear transport factor (karyopherin)	Nuclear transport factor (karyopherin); involved in protein transport between the cytoplasm and nucleoplasm; similar to Nmd5p, Cse1p, Lph2p, and the human cellular apoptosis susceptibility protein, CAS1
S000002805	YDR397C	NCB2	Negative Cofactor B	1266366	1266898	-1	Subunit of a heterodimeric NC2 transcription regulator complex	Subunit of a heterodimeric NC2 transcription regulator complex; complex binds to TBP and can repress transcription by preventing preinitiation complex assembly or stimulate activated transcription; homologous to human NC2beta; complex also includes Bur6p
S000002806	YDR398W	UTP5	U Three Protein	1267471	1269402	1	Subunit of U3-containing Small Subunit (SSU) processome complex	Subunit of U3-containing Small Subunit (SSU) processome complex; involved in production of 18S rRNA and assembly of small ribosomal subunit
S000002807	YDR399W	HPT1	Hypoxanthine guanine PhosphoribosylTransferase	1270068	1270733	1	Dimeric hypoxanthine-guanine phosphoribosyltransferase	Dimeric hypoxanthine-guanine phosphoribosyltransferase; catalyzes the transfer of the phosphoribosyl portion of 5-phosphoribosyl-alpha-1-pyrophosphate to a purine base (either guanine or hypoxanthine) to form pyrophosphate and a purine nucleotide (either guanosine monophosphate or inosine monophosphate); mutations in the human homolog HPRT1 can cause Lesch-Nyhan syndrome and Kelley-Seegmiller syndrome
S000002808	YDR400W	URH1	URidine Hydrolase	1271063	1272085	1	Uridine nucleosidase (uridine-cytidine N-ribohydrolase)	Uridine nucleosidase (uridine-cytidine N-ribohydrolase); cleaves N-glycosidic bonds in nucleosides; involved in the pyrimidine salvage and nicotinamide riboside salvage pathways
S000002809	YDR401W	""	""	1272223	1272786	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000002810	YDR402C	DIT2	DITyrosine	1272234	1273703	-1	N-formyltyrosine oxidase	N-formyltyrosine oxidase; sporulation-specific microsomal enzyme involved in the production of N,N-bisformyl dityrosine required for spore wall maturation, homologous to cytochrome P-450s; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum and vacuole respectively
S000002811	YDR403W	DIT1	DITyrosine	1274602	1276212	1	Sporulation-specific enzyme required for spore wall maturation	Sporulation-specific enzyme required for spore wall maturation; involved in the production of a soluble LL-dityrosine-containing precursor of the spore wall; transcripts accumulate at the time of prospore enclosure
S000002812	YDR404C	RPB7	RNA Polymerase B	1276654	1277169	-1	RNA polymerase II subunit B16	RNA polymerase II subunit B16; forms dissociable heterodimer with Rpb4p; Rpb4/7 dissociates from RNAPII as Ser2 CTD phosphorylation increases; Rpb4/7 regulates cellular lifespan via mRNA decay process; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex, export of mRNA to cytoplasm under stress conditions; also involved in translation initiation
S000002813	YDR405W	MRP20	Mitochondrial Ribosomal Protein	1277646	1278437	1	Mitochondrial ribosomal protein of the large subunit	Mitochondrial ribosomal protein of the large subunit
S000002814	YDR406W	PDR15	Pleiotropic Drug Resistance	1279210	1283799	1	Plasma membrane ATP binding cassette (ABC) transporter	Plasma membrane ATP binding cassette (ABC) transporter; multidrug transporter and general stress response factor implicated in cellular detoxification; regulated by Pdr1p, Pdr3p and Pdr8p; promoter contains a PDR responsive element; PDR15 has a paralog, PDR5, that arose from the whole genome duplication
S000002815	YDR407C	TRS120	TRapp Subunit	1284069	1287938	-1	Component of transport protein particle (TRAPP) complex II	Component of transport protein particle (TRAPP) complex II; TRAPPII is a multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating intra-Golgi and endosome-Golgi traffic
S000002816	YDR408C	ADE8	ADEnine requiring	1288215	1288859	-1	Phosphoribosyl-glycinamide transformylase	Phosphoribosyl-glycinamide transformylase; catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway
S000002817	YDR409W	SIZ1	SAP and mIZ-finger domain	1289406	1292120	1	SUMO E3 ligase	SUMO E3 ligase; promotes attachment of small ubiquitin-related modifier sumo (Smt3p) to primarily cytoplasmic proteins; regulates Rsp5p ubiquitin ligase activity and is in turn itself regulated by Rsp5p; required for sumoylation of septins and histone H3 variant Cse4p, a prerequisite for STUbL-mediated Ub-dependent degradation; localizes to the septin ring; acts as an adapter between E2, Ubc9p and substrates; tends to compensate for survival of DNA damage in absence of Nfi1p
S000002818	YDR410C	STE14	STErile	1292372	1293091	-1	Farnesyl cysteine-carboxyl methyltransferase	Farnesyl cysteine-carboxyl methyltransferase; mediates the carboxyl methylation step during C-terminal CAAX motif processing of a-factor and RAS proteins in the endoplasmic reticulum, localizes to the ER membrane
S000002819	YDR411C	DFM1	Der1-like Family Member	1293369	1294394	-1	Endoplasmic reticulum (ER) localized protein	Endoplasmic reticulum (ER) localized protein; involved in ER-associated protein degradation (ERAD), ER stress, and homeostasis; acts as disaggregase that removes misfolded membrane protein aggregates; interacts with components of ERAD-L and ERAD-C and Cdc48p; derlin-like family member similar to Der1p
S000002820	YDR412W	RRP17	Ribosomal RNA Processing	1294693	1295400	1	5' exoribonuclease required for 5' end processing of pre-60S rRNA	5' exoribonuclease required for 5' end processing of pre-60S rRNA; binds late pre-60S ribosomes, accompanying them from nucleolus to nuclear periphery; required for cell viability under standard (aerobic) conditions but not under anaerobic conditions; yeast null mutant is complemented by expression of human homolog NOL12
S000002822	YDR414C	ERD1	Endoplasmic reticulum Retention Defective	1295598	1296686	-1	Predicted membrane protein required for lumenal ER protein retention	Predicted membrane protein required for lumenal ER protein retention; Golgi inorganic phosphate (Pi) exporter that balances Pi import associated with NDP-sugar transport into the Golgi; involved in recycling of early glycosyltransferases from late to early Golgi compartments; mutants secrete the endogenous ER protein, BiP (Kar2p)
S000002823	YDR415C	""	""	1297037	1298161	-1	Putative aminopeptidase	Putative aminopeptidase; targeted to vacuole via Vps10p-dependent endosomal vacuolar protein sorting pathway
S000002825	YDR417C	""	""	1301555	1301926	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RPL12B/YDR418W
S000002826	YDR418W	RPL12B	Ribosomal Protein of the Large subunit	1301616	1302113	1	Ribosomal 60S subunit protein L12B	Ribosomal 60S subunit protein L12B; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12B has a paralog, RPL12A, that arose from the whole genome duplication
S000002827	YDR419W	RAD30	RADiation sensitive	1303174	1305072	1	DNA polymerase eta (pol eta)	DNA polymerase eta (pol eta); involved in translesion synthesis during post-replication repair; catalyzes synthesis of DNA opposite cyclobutane pyrimidine dimers and other lesions; involved in formation of post-replicative damage-induced genome-wide cohesion; may also have role in protection against mitochondrial mutagenesis; may possibly be involved in meiosis; mutations in human pol eta are responsible for XPV
S000002828	YDR420W	HKR1	Hansenula mrakii Killer toxin Resistant	1306267	1311675	1	Mucin family member that functions as an osmosensor in the HOG pathway	Mucin family member that functions as an osmosensor in the HOG pathway; large, highly glycosylated protein that functions redundantly with Msb2p in Sho1p-mediated osmostresss induction of the HOG signaling pathway; cytoplasmic domain interacts with Ahk1p a scaffold protein that prevents cross talk with the Kss1p MAPK of the filamentous growth pathway; mutant displays defects in beta-1,3 glucan synthesis and bud site selection
S000002829	YDR421W	ARO80	AROmatic amino acid requiring	1312040	1314892	1	Zinc finger transcriptional activator of the Zn2Cys6 family	Zinc finger transcriptional activator of the Zn2Cys6 family; activates transcription of aromatic amino acid catabolic genes in the presence of aromatic amino acids
S000002830	YDR422C	SIP1	SNF1-Interacting Protein	1315326	1317773	-1	One of three alternate beta-subunits of the Snf1p kinase complex	One of three alternate beta-subunits of the Snf1p kinase complex; may confer substrate specificity; vacuolar protein containing KIS (Kinase-Interacting Sequence) and ASC (Association with Snf1 kinase Complex) domains involved in protein interactions
S000002831	YDR423C	CAD1	CADmium resistance	1318046	1319275	-1	AP-1-like basic leucine zipper (bZIP) transcriptional activator	AP-1-like basic leucine zipper (bZIP) transcriptional activator; involved in stress responses, iron metabolism, and pleiotropic drug resistance; controls a set of genes involved in stabilizing proteins; binds consensus sequence TTACTAA; CAD1 has a paralog, YAP1, that arose from the whole genome duplication
S000002832	YDR424C	DYN2	DYNein	1319387	1319841	-1	Cytoplasmic light chain dynein, microtubule motor protein	Cytoplasmic light chain dynein, microtubule motor protein; required for intracellular transport and cell division; involved in mitotic spindle positioning; forms complex with dynein intermediate chain Pac11p that promotes Dyn1p homodimerization, potentiates motor processivity; Dyn2p-Pac11p complex important for interaction of dynein motor complex with dynactin complex; acts as molecular glue to dimerize, stabilize Nup82-Nsp1-Nup159 complex module of cytoplasmic pore filaments
S000002833	YDR425W	SNX41	Sorting NeXin	1320064	1321941	1	Sorting nexin	Sorting nexin; involved in the retrieval of late-Golgi SNAREs from the post-Golgi endosome to the trans-Golgi network; interacts with Snx4p
S000002834	YDR426C	""	""	1321629	1322006	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SNX41
S000002836	YDR428C	BNA7	Biosynthesis of NAD	1323454	1324239	-1	Formylkynurenine formamidase	Formylkynurenine formamidase; involved in the de novo biosynthesis of NAD from tryptophan via kynurenine
S000002837	YDR429C	TIF35	Translation Initiation Factor	1324477	1325301	-1	eIF3g subunit of the eukaryotic translation initiation factor 3 (eIF3)	eIF3g subunit of the eukaryotic translation initiation factor 3 (eIF3); subunit of the core complex of eIF3; is essential for translation; stimulates resumption of ribosomal scanning during translation reinitiation; eIF3 is also involved in programmed stop codon readthrough
S000002838	YDR430C	CYM1	CYtosolic Metalloprotease	1325501	1328470	-1	Lysine-specific metalloprotease of the pitrilysin family	Lysine-specific metalloprotease of the pitrilysin family; metalloprotease of the intermembrane space; degrades proteins and presequence peptides cleaved from imported proteins; required for normal mitochondrial morphology; mutation in human homolog PITRM1 is implicated in autosomal recessive spinocerebellar ataxias (ARCA)
S000002839	YDR431W	""	""	1328390	1328701	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000002840	YDR432W	NPL3	Nuclear Protein Localization	1328783	1330027	1	RNA-binding protein	RNA-binding protein; promotes elongation, regulates termination, and is involved in nuclear export of poly(A) mRNA; represses translation initiation by binding eIF4G; required for pre-mRNA splicing; interacts with E3 ligase Bre1p, linking histone ubiquitination to mRNA processing; binds TERRA and stabilizes R-loops at short telomeres; phosphorylation by Sky1p and interaction with Mtr10p promotes shuttling and mRNA dissociation; protein abundance increases in response to DNA replication stress
S000002841	YDR433W	""	""	1329600	1330040	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000002842	YDR434W	GPI17	GlycosylPhosphatidylInositol anchor biosynthesis	1331237	1332841	1	Transmembrane protein	Transmembrane protein; subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-S homolog
S000002843	YDR435C	PPM1	Protein Phosphatase Methyltransferase	1332983	1333969	-1	Carboxyl methyltransferase	Carboxyl methyltransferase; methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits; required for methionine to inhibit autophagy and promote growth
S000002844	YDR436W	PPZ2	Protein Phosphatase Z	1334821	1336953	1	Serine/threonine protein phosphatase Z, isoform of Ppz1p	Serine/threonine protein phosphatase Z, isoform of Ppz1p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance
S000002845	YDR437W	GPI19	Glycosyl PhosphatidylInositol anchor biosynthesis	1337352	1337774	1	Subunit of GPI-GlcNAc transferase involved in synthesis of GlcNAc-PI	Subunit of GPI-GlcNAc transferase involved in synthesis of GlcNAc-PI; N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI) is the first intermediate in glycosylphosphatidylinositol (GPI) anchor synthesis; shares similarity with mammalian PIG-P
S000002846	YDR438W	THI74	THIamine regulon	1338274	1339386	1	Mitochondrial transporter repressible by thiamine	Mitochondrial transporter repressible by thiamine; THI74 has a paralog, YML018C, that arose from the whole genome duplication; shows sequence homology to human gene SLC35F3, a thiamine transporter implicated in hypertension
S000002847	YDR439W	LRS4	Loss of RDNA Silencing	1339676	1340719	1	Nucleolar protein that forms a complex with Csm1p	Nucleolar protein that forms a complex with Csm1p; and then Mam1p at kinetochores during meiosis I to mediate accurate homolog segregation; required for condensin recruitment to the replication fork barrier site and rDNA repeat segregation
S000002848	YDR440W	DOT1	Disruptor Of Telomeric silencing	1342493	1344241	1	Nucleosomal histone H3-Lys79 methylase	Nucleosomal histone H3-Lys79 methylase; methylation is required for telomeric silencing, meiotic checkpoint control, and DNA damage response; single DOT1 gene produces two Dot1p isoforms from alternative translation start sites as a result of leaky scanning by the ribosome
S000002849	YDR441C	APT2	Adenine PhosphoribosylTransferase	1344517	1345062	-1	Potential adenine phosphoribosyltransferase	Potential adenine phosphoribosyltransferase; encodes a protein with similarity to adenine phosphoribosyltransferase, but artificially expressed protein exhibits no enzymatic activity; APT2 has a paralog, APT1, that arose from the whole genome duplication
S000002850	YDR442W	""	""	1345647	1346039	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000002851	YDR443C	SSN2	Suppressor of SNf1	1345676	1349938	-1	Subunit of the RNA polymerase II mediator complex	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for stable association of Srb10p-Srb11p kinase; essential for transcriptional regulation
S000002852	YDR444W	""	""	1350290	1352353	1	Putative hydrolase acting on ester bonds	Putative hydrolase acting on ester bonds
S000002853	YDR445C	""	""	1352182	1352589	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000002854	YDR446W	ECM11	ExtraCellular Mutant	1353725	1354633	1	Meiosis-specific protein	Meiosis-specific protein; component of the Synaptonemal Complex (SC) along with Gmc2p; required for efficient crossover formation and for the efficient loading of the SC transverse filament protein, Zip1p; is SUMOlytaed in a Gmc2p manner, and SUMOylation is required for its function in meiosis; GFP fusion protein is present in discrete clusters in the nucleus throughout mitosis; may be involved in maintaining chromatin structure
S000002855	YDR447C	RPS17B	Ribosomal Protein of the Small subunit	1354829	1355553	-1	Ribosomal protein 51 (rp51) of the small (40s) subunit	Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17B has a paralog, RPS17A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
S000002856	YDR448W	ADA2	transcriptional ADAptor	1356065	1357369	1	Transcription coactivator	Transcription coactivator; component of the ADA and SAGA transcriptional adaptor/HAT (histone acetyltransferase) complexes
S000002858	YDR450W	RPS18A	Ribosomal Protein of the Small subunit	1359923	1360798	1	Protein component of the small (40S) ribosomal subunit	Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18A has a paralog, RPS18B, that arose from the whole genome duplication; protein increases in abundance and relocalizes from cytoplasm to nuclear foci upon DNA replication stress
S000002859	YDR451C	YHP1	Yeast Homeo-Protein	1361120	1362181	-1	Homeobox transcriptional repressor	Homeobox transcriptional repressor; binds Mcm1p and early cell cycle box (ECB) elements of cell cycle regulated genes, thereby restricting ECB-mediated transcription to the M/G1 interval; YHP1 has a paralog, YOX1, that arose from the whole genome duplication
S000002860	YDR452W	PPN1	""	1362878	1364902	1	Dual endo- and exopolyphosphatase with a role in phosphate metabolism	Dual endo- and exopolyphosphatase with a role in phosphate metabolism; acts as both an endopolyphosphatase cleaving long chains of polyphosphate distributively to generate shorter polymer chains and as an exopolyphosphatase catalyzing the hydrolysis of terminal phosphate from polyphosphate; localizes to the vacuole, nucleus and cytosol; functions as a homodimer; relocalizes from vacuole to cytoplasm upon DNA replication stress
S000002861	YDR453C	TSA2	Thiol-Specific Antioxidant	1365072	1365662	-1	Stress inducible cytoplasmic thioredoxin peroxidase	Stress inducible cytoplasmic thioredoxin peroxidase; cooperates with Tsa1p in the removal of reactive oxygen, nitrogen and sulfur species using thioredoxin as hydrogen donor; deletion enhances the mutator phenotype of tsa1 mutants; protein abundance increases in response to DNA replication stress; TSA2 has a paralog, TSA1, that arose from the whole genome duplication
S000002862	YDR454C	GUK1	GUanylate Kinase	1366264	1366827	-1	Guanylate kinase	Guanylate kinase; converts GMP to GDP; required for growth and mannose outer chain elongation of cell wall N-linked glycoproteins
S000002863	YDR455C	""	""	1367373	1367681	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YDR456W
S000002864	YDR456W	NHX1	Na+/H+ eXchanger	1367485	1369386	1	Na+/H+ and K+/H+ exchanger	Na+/H+ and K+/H+ exchanger; required for intracellular sequestration of Na+ and K+; located in the vacuole and late endosome compartments; required for osmotolerance to acute hypertonic shock and for vacuolar fusion; ortholog of human NHE9, which is linked to autism
S000002865	YDR457W	TOM1	Temperature dependent Organization in Mitotic nucleus or Trigger Of Mitosis	1369790	1379596	1	E3 ubiquitin ligase of the hect-domain class	E3 ubiquitin ligase of the hect-domain class; has a role in mRNA export from the nucleus and may regulate transcriptional coactivators; involved in degradation of excess histones; interacts with Dia2p and is required for Dia2p degradation; required to target Cdc6p for ubiquitin-mediated destruction during G1 phase
S000002866	YDR458C	HEH2	Helix-Extension-Helix domain	1380055	1382046	-1	Inner nuclear membrane (INM) protein	Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle; HEH2 has a paralog, SRC1, that arose from the whole genome duplication
S000002867	YDR459C	PFA5	Protein Fatty Acyltransferase	1382319	1383443	-1	Palmitoyltransferase with autoacylation activity	Palmitoyltransferase with autoacylation activity; likely functions in pathway(s) outside Ras; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain
S000002868	YDR460W	TFB3	Transcription Factor B	1383811	1384776	1	Subunit of TFIIH and nucleotide excision repair factor 3 complexes	Subunit of TFIIH and nucleotide excision repair factor 3 complexes; involved in transcription initiation, required for nucleotide excision repair; ring finger protein similar to mammalian CAK and TFIIH subunit
S000002869	YDR461W	MFA1	Mating Factor A	1385176	1385286	1	Mating pheromone a-factor	Mating pheromone a-factor; made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA2
S000002870	YDR462W	MRPL28	Mitochondrial Ribosomal Protein, Large subunit	1386073	1386516	1	Mitochondrial ribosomal protein of the large subunit	Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress
S000002871	YDR463W	STP1	Species-specific tRNA Processing	1386816	1388375	1	Transcription factor	Transcription factor; contains a N-terminal regulatory motif (RI) that acts as a cytoplasmic retention determinant and as an Asi dependent degron in the nucleus; undergoes proteolytic processing by SPS (Ssy1p-Ptr3p-Ssy5p)-sensor component Ssy5p in response to extracellular amino acids; activates transcription of amino acid permease genes and may have a role in tRNA processing; STP1 has a paralog, STP2, that arose from the whole genome duplication
S000002872	YDR464W	SPP41	Suppressor of PrP4	1388872	1393179	1	Protein of unknown function	Protein of unknown function; involved in negative regulation of expression of spliceosome components PRP4 and PRP3; relocalizes to the cytosol in response to hypoxia
S000002873	YDR465C	RMT2	aRginine MeThyltransferase	1393336	1394574	-1	Arginine N5 methyltransferase	Arginine N5 methyltransferase; methylates ribosomal protein Rpl12 (L12) on Arg67; relative distribution to the nucleus increases upon DNA replication stress
S000002874	YDR466W	PKH3	Pkb-activating Kinase Homolog	1395121	1397817	1	Protein kinase with similarity to mammalian PDK1 and yeast Pkh1p/Phk2p	Protein kinase with similarity to mammalian PDK1 and yeast Pkh1p/Phk2p; yeast Pkh1p and Pkh2p are two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant
S000002875	YDR467C	""	""	1397583	1397909	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000002876	YDR468C	TLG1	T-snare affecting a Late Golgi compartment	1398026	1398700	-1	Essential t-SNARE that mediates fusion of vesicles with the late Golgi	Essential t-SNARE that mediates fusion of vesicles with the late Golgi; forms a complex with Tlg2p and Vti1p; mediates fusion of endosome-derived vesicles with the late Golgi; binds the docking complex VFT (Vps fifty-three) through interaction with Vps51p
S000002877	YDR469W	SDC1	Set1c, homolog of Dpy30 from C.elegans	1399015	1399542	1	Subunit of the COMPASS (Set1C) complex	Subunit of the COMPASS (Set1C) complex; COMPASS methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30
S000002878	YDR470C	UGO1	UGO (Japanese for fusion)	1399706	1401214	-1	Outer membrane component of the mitochondrial fusion machinery	Outer membrane component of the mitochondrial fusion machinery; binds to Fzo1p and Mgm1p to link these two GTPases during mitochondrial fusion; involved in fusion of both the outer and inner membranes; facilitates dimerization of Fzo1p during fusion; import into the outer membrane is mediated by Tom70p and Mim1p; has similarity to carrier proteins but likely not a transporter; similar to human SLC25A46 implicated in optic atroprophy spectrum disorder and Leigh syndrome
S000002879	YDR471W	RPL27B	Ribosomal Protein of the Large subunit	1401770	1402564	1	Ribosomal 60S subunit protein L27B	Ribosomal 60S subunit protein L27B; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27B has a paralog, RPL27A, that arose from the whole genome duplication
S000002880	YDR472W	TRS31	TRapp Subunit	1403322	1404173	1	Core component of transport protein particle (TRAPP) complexes I-III	Core component of transport protein particle (TRAPP) complexes I-III; TRAPP complexes are related multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating ER-Golgi traffic (TRAPPI), intra-Golgi traffic (TRAPPII), endosome-Golgi traffic (TRAPPII and III) and autophagy (TRAPPIII)
S000002881	YDR473C	PRP3	Pre-mRNA Processing	1404445	1405854	-1	Splicing factor	Splicing factor; component of the U4/U6-U5 snRNP complex
S000002882	YDR474C	""	""	1407452	1409119	-1	Merged open reading frame	Merged open reading frame; does not encode a discrete protein; YDR474C was originally annotated as an independent ORF, but as a result of a sequence change, it was merged with an adjacent ORF into a single reading frame, designated YDR475C
S000002883	YDR475C	JIP4	Jumonji domain Interacting Protein	1407464	1410094	-1	Protein of unknown function	Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence; JIP4 has a paralog, YOR019W, that arose from the whole genome duplication
S000002884	YDR476C	""	""	1410453	1411127	-1	Putative protein of unknown function	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YDR476C is not an essential gene
S000002885	YDR477W	SNF1	Sucrose NonFermenting	1412373	1414274	1	AMP-activated S/T protein kinase (AMPK)	AMP-activated S/T protein kinase (AMPK); complexes with Snf4p and a Sip1p/Sip2p/Gal83p family member; required for glucose-shortage induced respiratory gene expression, heat shock, sporulation, and peroxisome biogenesis; regulates filamentous growth and acts as a non-canonical GEF activating Arf3p during invasive growth; involved in mitotic spindle alignment in non-limiting glucose; target of sumoylation dependent Ub-mediated destruction; polyhistidine tract acts as a pH sensing module
S000002886	YDR478W	SNM1	Suppressor of Nuclear Mitochondrial endoribonuclease	1414575	1415171	1	Ribonuclease MRP complex subunit	Ribonuclease MRP complex subunit; ribonuclease (RNase) MRP cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP
S000002887	YDR479C	PEX29	PEroXisome related	1415210	1416874	-1	ER-resident protein involved in peroxisomal biogenesis	ER-resident protein involved in peroxisomal biogenesis; ER-localized protein that associates with peroxisomes; interacts with Pex30p and reticulons Rtn1p and Yop1p to regulate peroxisome biogenesis from the ER; role in peroxisomal-destined vesicular flow from the ER; regulates peroxisomal size, number and distribution; Pex28p and Pex29p may act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p; forms ER foci upon DNA replication stress
S000002888	YDR480W	DIG2	Down-regulator of Invasive Growth	1417399	1418370	1	MAP kinase-responsive inhibitor of the Ste12p transcription factor	MAP kinase-responsive inhibitor of the Ste12p transcription factor; involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p; DIG2 has a paralog, DIG1, that arose from the whole genome duplication
S000002889	YDR481C	PHO8	PHOsphate metabolism	1418550	1420250	-1	Repressible vacuolar alkaline phosphatase	Repressible vacuolar alkaline phosphatase; controls polyphosphate content; regulated by levels of Pi and by Pho4p, Pho9p, Pho80p, Pho81p and Pho85p; dephosphorylates phosphotyrosyl peptides; contributes to NAD+ metabolism by producing nicotinamide riboside from NMN
S000002890	YDR482C	CWC21	Complexed With Cef1p	1420431	1420838	-1	Protein involved in RNA splicing by the spliceosome	Protein involved in RNA splicing by the spliceosome; component of a complex containing Cef1p; interacts genetically with ISY1 and BUD13; may bind RNA; has similarity to S. pombe Cwf21p
S000002891	YDR483W	KRE2	Killer toxin REsistant	1421157	1422485	1	Alpha1,2-mannosyltransferase of the Golgi	Alpha1,2-mannosyltransferase of the Golgi; involved in protein mannosylation; KRE2 has a paralog, KTR6, that arose from the whole genome duplication
S000002892	YDR484W	VPS52	Vacuolar Protein Sorting	1422763	1424688	1	Component of the GARP (Golgi-associated retrograde protein) complex	Component of the GARP (Golgi-associated retrograde protein) complex; GARP is required for the recycling of proteins from endosomes to the late Golgi, and for mitosis after DNA damage induced checkpoint arrest; involved in localization of actin and chitin; members of the GARP complex are Vps51p-Vps52p-Vps53p-Vps54p
S000002893	YDR485C	VPS72	Vacuolar Protein Sorting	1424820	1427207	-1	Htz1p-binding component of the SWR1 complex	Htz1p-binding component of the SWR1 complex; exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; may function as a lock that prevents removal of H2AZ from nucleosomes; required for vacuolar protein sorting
S000002894	YDR486C	VPS60	Vacuolar Protein Sorting	1427431	1428120	-1	Protein involved in late endosome to vacuole transport	Protein involved in late endosome to vacuole transport; cytoplasmic and vacuolar membrane protein; required for normal filament maturation during pseudohyphal growth; may function in targeting cargo proteins for degradation; interacts with Vta1p
S000002895	YDR487C	RIB3	RIBoflavin biosynthesis	1428354	1428980	-1	3,4-dihydroxy-2-butanone-4-phosphate synthase (DHBP synthase)	3,4-dihydroxy-2-butanone-4-phosphate synthase (DHBP synthase); required for riboflavin biosynthesis from ribulose-5-phosphate, also has an unrelated function in mitochondrial respiration
S000002896	YDR488C	PAC11	Perish in the Absence of CIN8	1429188	1430789	-1	Dynein intermediate chain, microtubule motor protein	Dynein intermediate chain, microtubule motor protein; required for intracellular transport and cell division; acts in cytoplasmic dynein pathway; forms complex with dynein light chain Dyn2p that promotes Dyn1p homodimerization and potentiates motor processivity; Dyn2p-Pac11p complex is also important for interaction of dynein motor complex with dynactin complex; forms cortical cytoplasmic microtubule capture site with Num1p; essential in the absence of CIN8
S000002898	YDR490C	PKH1	Pkb-activating Kinase Homolog	1431968	1434268	-1	Serine/threonine protein kinase	Serine/threonine protein kinase; involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ypk2p, components of signaling cascade required for maintenance of cell wall integrity; contains a PH-like domain; redundant with Pkh2p; PKH1 has a paralog, PKH2, that arose from the whole genome duplication
S000002899	YDR491C	""	""	1434500	1434991	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000002900	YDR492W	IZH1	Implicated in Zinc Homeostasis	1434924	1435874	1	Membrane protein involved in zinc ion homeostasis	Membrane protein involved in zinc ion homeostasis; member of the four-protein IZH family; transcription is regulated directly by Zap1p, expression induced by zinc deficiency and fatty acids; deletion increases sensitivity to elevated zinc; IZH1 has a paralog, IZH4, that arose from the whole genome duplication
S000002901	YDR493W	MZM1	Mitochondrial Zinc Maintenance	1436217	1436588	1	Protein required for assembly of the cytochrome bc(1) complex	Protein required for assembly of the cytochrome bc(1) complex; acts as a chaperone for Rip1p and facilitates its insertion into the complex at a late stage of assembly; localized to the mitochondrial matrix; null mutant exhibits a respiratory growth defect and reduced mitochondrial zinc levels, which is characteristic of mutations affecting bc(1) complex assembly; member of the LYR protein family; human LYRM7 is a functional ortholog
S000002902	YDR494W	RSM28	Ribosomal Small subunit of Mitochondria	1436930	1438015	1	Mitochondrial ribosomal protein of the small subunit	Mitochondrial ribosomal protein of the small subunit; genetic interactions suggest a possible role in promoting translation initiation
S000002903	YDR495C	VPS3	Vacuolar Protein Sorting	1438115	1441150	-1	Component of CORVET membrane tethering complex	Component of CORVET membrane tethering complex; cytoplasmic protein required for the sorting and processing of soluble vacuolar proteins, acidification of the vacuolar lumen, and assembly of the vacuolar H+-ATPase
S000002904	YDR496C	PUF6	PUmilio-homology domain Family	1441433	1443403	-1	Pumilio-homology domain protein	Pumilio-homology domain protein; binds the 3' UTR of ASH1 mRNA and represses its translation, resulting in proper asymmetric localization of ASH1 mRNA; required at post-transcriptional step for efficient retrotransposition; absence results in decreased Ty1 Gag:GFP protein levels; co-sediments with the 60S ribosomal subunit and is required for its biogenesis
S000002905	YDR497C	ITR1	myo-Inositol TRansporter	1443713	1445467	-1	Myo-inositol transporter	Myo-inositol transporter; member of the sugar transporter superfamily; expression is repressed by inositol and choline via Opi1p and derepressed via Ino2p and Ino4p; relative distribution to the vacuole increases upon DNA replication stress; ITR1 has a paralog, ITR2, that arose from the whole genome duplication
S000002906	YDR498C	SEC20	SECretory	1445843	1446994	-1	Membrane glycoprotein v-SNARE	Membrane glycoprotein v-SNARE; involved in retrograde transport from the Golgi to the endoplasmic reticulum (ER); required for N- and O-glycosylation in the Golgi but not in the ER and for efficient nuclear fusion during mating; mediates Sey1p-independent homotypic ER fusion; interacts with the Dsl1p complex through Tip20p
S000002907	YDR499W	LCD1	Lethal, Checkpoint-defective, DNA damage sensitive	1447830	1450073	1	Essential protein required for the DNA integrity checkpoint pathways	Essential protein required for the DNA integrity checkpoint pathways; interacts physically with Mec1p and contributes to its activation; putative homolog of S. pombe Rad26 and human ATRIP; forms nuclear foci upon DNA replication stress
S000002908	YDR500C	RPL37B	Ribosomal Protein of the Large subunit	1450198	1450853	-1	Ribosomal 60S subunit protein L37B	Ribosomal 60S subunit protein L37B; required for processing of 27SB pre-rRNA and formation of stable 66S assembly intermediates; protein abundance increases in response to DNA replication stress; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37B has a paralog, RPL37A, that arose from the whole genome duplication
S000002909	YDR501W	PLM2	PLasmid Maintenance	1451353	1452918	1	Putative transcription factor, contains Forkhead Associated domain	Putative transcription factor, contains Forkhead Associated domain; found associated with chromatin; target of SBF transcription factor; induced in response to DNA damaging agents and deletion of telomerase; PLM2 has a paralog, TOS4, that arose from the whole genome duplication
S000002910	YDR502C	SAM2	S-AdenosylMethionine requiring	1453310	1454464	-1	S-adenosylmethionine synthetase	S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; comparative analysis suggests that a mitochondrially targeted form may result from translation starting at a non-canonical codon upstream of the annotated start codon
S000002911	YDR503C	LPP1	Lipid Phosphate Phosphatase	1455042	1455866	-1	Lipid phosphate phosphatase	Lipid phosphate phosphatase; catalyzes Mg(2+)-independent dephosphorylation of phosphatidic acid (PA), lysophosphatidic acid, and diacylglycerol pyrophosphate; involved in control of the cellular levels of phosphatidylinositol and PA
S000002912	YDR504C	SPG3	Stationary Phase Gene	1456311	1456694	-1	Protein required for high temperature survival during stationary phase	Protein required for high temperature survival during stationary phase; not required for growth on nonfermentable carbon sources; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum
S000002913	YDR505C	PSP1	Polymerase SuPpressor	1456695	1459220	-1	Asn and gln rich protein of unknown function	Asn and gln rich protein of unknown function; high-copy suppressor of POL1 (DNA polymerase alpha) and partial suppressor of CDC2 (polymerase delta) and CDC6 (pre-RC loading factor) mutations; overexpression results in growth inhibition; capable of forming the prion [PSP1+]; PSP1 has a paralog, YLR177W, that arose from the whole genome duplication
S000002914	YDR506C	GMC1	Grand Meiotic recombination Cluster	1459728	1461554	-1	Protein involved in meiotic progression	Protein involved in meiotic progression; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; possible membrane-localized protein; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum and vacuole respectively
S000002915	YDR507C	GIN4	Growth Inhibitory	1462358	1465786	-1	Protein kinase involved in bud growth and assembly of the septin ring	Protein kinase involved in bud growth and assembly of the septin ring; proposed to have kinase-dependent and kinase-independent activities; negatively regulates Fpk1p kinase that stimulates phospholipid flippases Dnf1p and Dnf2p; undergoes autophosphorylation; similar to Hsl1p; GIN4 has a paralog, KCC4, that arose from the whole genome duplication
S000002916	YDR508C	GNP1	GlutamiNe Permease	1466453	1468444	-1	Broad specificity amino acid permease	Broad specificity amino acid permease; high-affinity glutamine permease; major serine permease with minor contributions from paralog Agp1p; role in serine uptake impacts cellular sphingolipid homeostasis; also transports Leu, Thr, Cys, Met and Asn; expression is fully dependent on Grr1p and modulated by the Ssy1p-Ptr3p-Ssy5p (SPS) sensor of extracellular amino acids
S000002917	YDR509W	""	""	1468226	1468573	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000002918	YDR510W	SMT3	Suppressor of Mif Two	1469400	1469705	1	Ubiquitin-like protein of the SUMO family	Ubiquitin-like protein of the SUMO family; conjugated to lysine residues of target proteins; associates with transcriptionally active genes; regulates chromatid cohesion, chromosome segregation, APC-mediated proteolysis, DNA replication and septin ring dynamics; human homolog SUMO1 can complement yeast null mutant
S000002919	YDR511W	SDH7	Succinate DeHydrogenase	1470017	1470418	1	Mitochondrial protein involved in assembly of succinate dehydrogenase	Mitochondrial protein involved in assembly of succinate dehydrogenase; has a role in maturation of the Sdh2p subunit; localized to the mitochondrial intermembrane space; required for acetate utilization and gluconeogenesis; mutation in Drosophila ortholog SDHAF3 causes reduced succinate dehydrogenase activity and neuronal and muscular dysfunction; member of the LYR protein family
S000002920	YDR512C	EMI1	Early Meiotic Induction	1470500	1471063	-1	Non-essential protein of unknown function	Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1, also required for sporulation; contains twin cysteine-x9-cysteine motifs; deletion affects mitochondrial morphology
S000002921	YDR513W	GRX2	GlutaRedoXin	1471017	1471448	1	Cytoplasmic glutaredoxin	Cytoplasmic glutaredoxin; thioltransferase, glutathione-dependent disulfide oxidoreductase involved in maintaining redox state of target proteins, also exhibits glutathione peroxidase activity, expression induced in response to stress; GRX2 has two in-frame start codons resulting in a shorter isoform that is retained in the cytosol and a longer form translocated to the mitochondrial matrix; GRX2 has a paralog, GRX1, that arose from the whole genome duplication
S000002922	YDR514C	""	""	1471548	1472999	-1	Protein of unknown function that localizes to mitochondria	Protein of unknown function that localizes to mitochondria; overexpression affects endocytic protein trafficking; YDR514C has a paralog, GFD2, that arose from the whole genome duplication
S000002923	YDR515W	SLF1	SuLFide production	1473429	1474772	1	RNA binding protein that associates with polysomes	RNA binding protein that associates with polysomes; may be involved in regulating mRNA translation; involved in the copper-dependent mineralization of copper sulfide complexes on cell surface in cells cultured in copper salts; role in preventing L-A mycovirus pathogenesis; SLF1 has a paralog, SRO9, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
S000002924	YDR516C	EMI2	Early Meiotic Induction	1474974	1476476	-1	Hexokinase expressed during growth with low glucose levels	Hexokinase expressed during growth with low glucose levels; induced on non-fermentable carbon sources; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; protein abundance increases in response to DNA replication stress; EMI2 has a paralog, GLK1, that arose from the whole genome duplication
S000002925	YDR517W	GRH1	GRasp65 (Golgi reassembly stacking protein of 65kD) Homolog	1477239	1478357	1	Acetylated cis-Golgi protein, homolog of human GRASP65	Acetylated cis-Golgi protein, homolog of human GRASP65; forms a complex with the coiled-coil protein Bug1p; required for unconventional protein secretion (UPS) of Acb1p in response to starvation; forms amyloid fibrils under heat shock; protein abundance increases in response to DNA replication stress
S000002926	YDR518W	EUG1	ER protein Unnecessary for Growth	1478608	1480161	1	Protein disulfide isomerase of the endoplasmic reticulum lumen	Protein disulfide isomerase of the endoplasmic reticulum lumen; EUG1 has a paralog, PDI1, that arose from the whole genome duplication; function overlaps with that of Pdi1p; may interact with nascent polypeptides in the ER
S000002927	YDR519W	FPR2	FKBP Proline Rotamase	1480425	1480832	1	Membrane-bound peptidyl-prolyl cis-trans isomerase (PPIase)	Membrane-bound peptidyl-prolyl cis-trans isomerase (PPIase); binds to the drugs FK506 and rapamycin; expression pattern suggests possible involvement in ER protein trafficking; relocalizes from nucleus to vacuole upon DNA replication stress; mutation is functionally complemented by human FKBP2
S000002928	YDR520C	URC2	URacil Catabolism	1481085	1483403	-1	Putative Zn(II)2Cys6 motif containing transcription factor	Putative Zn(II)2Cys6 motif containing transcription factor; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; similar to S. kluyveri Urc2p involved in uracil catabolism
S000002929	YDR521W	""	""	1483141	1483476	1	Dubious ORF that overlaps YDR520C	Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol
S000002930	YDR522C	SPS2	SPorulation Specific	1483795	1485303	-1	Protein expressed during sporulation	Protein expressed during sporulation; SPS2 has a paralog, SPS22, that arose from the whole genome duplication; redundant with Sps22p for organization of the beta-glucan layer of the spore wall; S. pombe ortholog is a spore wall component
S000002931	YDR523C	SPS1	SPorulation Specific	1485566	1487038	-1	STE20-family GCKIII protein kinase	STE20-family GCKIII protein kinase; required for prospore membrane closure, efficient spore packaging, and localization of enzymes involved in spore wall synthesis; component of a non-canonical Hippo pathway with Cdc15p involved in spindle disassembly and sustained release of Cdc14p during anaphase II; forms a complex with Cdc15p;  phosphorylation requires Cdc15p; required for Ssp1p phosphorylation and turnover; interacts with 14-3-3 proteins; expressed in late meiosis; nuclear and cytoplasmic
S000002932	YDR524C	AGE1	Arf Gap with Effector function(s)	1487542	1488990	-1	ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector	ADP-ribosylation factor (ARF) GTPase activating protein (GAP) effector; involved in the secretory and endocytic pathways; contains C2C2H2 cysteine/histidine motif
S000002933	YDR525W	API2	APIcal	1489905	1490234	1	Putative protein of unknown function	Putative protein of unknown function; conserved among S. cerevisiae strains; not conserved in closely related Saccharomyces species; 26% of ORF overlaps the dubious ORF YDR524C-A; insertion mutation in a cdc34-2 mutant background causes altered bud morphology
S000002934	YDR526C	""	""	1491075	1491545	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000002935	YDR527W	RBA50	RNA polymerase II (B) Associated protein	1491094	1492413	1	Protein involved in transcription	Protein involved in transcription; interacts with RNA polymerase II subunits Rpb2p, Rpb3, and Rpb11p; has similarity to human RPAP1
S000002936	YDR528W	HLR1	Homolog of LRe1	1494586	1495857	1	Protein involved in regulation of cell wall composition and integrity	Protein involved in regulation of cell wall composition and integrity; also involved in cell wall response to osmotic stress; overproduction suppresses a lysis sensitive PKC mutation; HLR1 has a paralog, LRE1, that arose from the whole genome duplication
S000002937	YDR529C	QCR7	ubiQuinol-cytochrome C oxidoReductase	1496165	1496548	-1	Subunit 7 of ubiquinol cytochrome-c reductase (Complex III)	Subunit 7 of ubiquinol cytochrome-c reductase (Complex III); Complex III is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly
S000002938	YDR530C	APA2	AP4A phosphorylase	1496791	1497768	-1	Diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase II	Diadenosine 5',5'''-P1,P4-tetraphosphate phosphorylase II; AP4A phosphorylase involved in catabolism of bis(5'-nucleosidyl) tetraphosphates; APA2 has a paralog, APA1, that arose from the whole genome duplication
S000002939	YDR531W	CAB1	Coenzyme A Biosynthesis	1498232	1499335	1	Pantothenate kinase, ATP:D-pantothenate 4'-phosphotransferase	Pantothenate kinase, ATP:D-pantothenate 4'-phosphotransferase; cytoplasmic enzyme that catalyzes the first committed step in the universal biosynthetic pathway for coenzyme A (CoA) synthesis; transcription is regulated by Upc2p via a sterol response element; overexpressed human homologs, PANK1 and PANK3, complement yeast hypomorphic alleles, while PANK2, a gene implicated in pantothenate-kinase associated neurodegeneration, partially complements after the mitochondrial transit peptide is removed
S000002940	YDR532C	KRE28	""	1499396	1500553	-1	Subunit of a kinetochore-microtubule binding complex	Subunit of a kinetochore-microtubule binding complex; complex bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components; complex also includes Spc105p; modified by sumoylation
S000002941	YDR533C	HSP31	Heat-Shock Protein	1501447	1502160	-1	Methylglyoxalase that converts methylglyoxal to D-lactate	Methylglyoxalase that converts methylglyoxal to D-lactate; involved in oxidative stress resistance, diauxic shift, and stationary phase survival; has similarity to E. coli Hsp31 and C. albicans Glx3p; member of the DJ-1/ThiJ/PfpI superfamily, which includes human DJ-1 involved in Parkinson's disease and cancer; exists as a dimer and contains a putative metal-binding site; protein abundance increases in response to DNA replication stress
S000002942	YDR534C	FIT1	Facilitator of Iron Transport	1503314	1504900	-1	Mannoprotein that is incorporated into the cell wall	Mannoprotein that is incorporated into the cell wall; incorporated via a glycosylphosphatidylinositol (GPI) anchor; involved in the retention of siderophore-iron in the cell wall 
S000002943	YDR535C	""	""	1506606	1507362	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YDR535C is not an essential gene
S000002944	YDR536W	STL1	Sugar Transporter-Like protein	1508005	1509714	1	Glycerol proton symporter of the plasma membrane	Glycerol proton symporter of the plasma membrane; subject to glucose-induced inactivation, strongly but transiently induced when cells are subjected to osmotic shock
S000002945	YDR537C	""	""	1510856	1511461	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps verified ORF PAD1/YDR538W
S000002946	YDR538W	PAD1	Phenylacrylic Acid Decarboxylase	1510902	1511630	1	Phenylacrylic acid decarboxylase	Phenylacrylic acid decarboxylase; decarboxylates aromatic carboxylic acids to the corresponding vinyl derivatives; confers resistance to cinnamic acid; overexpression of both Pad1p and Fdc1p increases cinnamic acid decarboxylase activity due to the Pad1p-catalyzed formation of a diffusible cofactor required for Fdc1p activity; contains mRNA binding activity; homolog of E. coli UbiX
S000002947	YDR539W	FDC1	Ferulic acid DeCarboxylase	1512094	1513605	1	Ferulic acid decarboxylase	Ferulic acid decarboxylase; involved in the decarboxylation of aromatic carboxylic acids such as phenylacrylic (cinnamic) acid, ferulic acid and coumaric acid and formation of the corresponding vinyl derivatives; overexpression of both Pad1p and Fdc1p increases decarboxylase activity due to the Pad1p-catalyzed formation of a diffusible cofactor required for Fdc1p activity; homolog of E. coli UbiD; GFP-fusion protein localizes to the cytoplasm in an HTP study
S000002948	YDR540C	IRC4	Increased Recombination Centers	1517136	1517675	-1	Protein similar to bacterial secreted DfsB proteins	Protein similar to bacterial secreted DfsB proteins; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
S000002949	YDR541C	""	""	1519664	1520698	-1	Aldehyde reductase	Aldehyde reductase; substrates include both aromatic and aliphatic aldehydes; uses NADPH as cofactor; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum and vacuole respectively
S000002954	YEL059C-A	SOM1	SOrting Mitochondrial	42400	42624	-1	Subunit of the mitochondrial inner membrane peptidase (IMP)	Subunit of the mitochondrial inner membrane peptidase (IMP); IMP is required for maturation of mitochondrial proteins of the intermembrane space; Som1p facilitates cleavage of a subset of substrates; contains twin cysteine-x9-cysteine motifs
S000002957	YER007C-A	TMA20	Translation Machinery Associated	166237	166885	-1	Protein of unknown function that associates with ribosomes	Protein of unknown function that associates with ribosomes; has a putative RNA binding domain; interacts with Tma22p; null mutant exhibits translation defects; has homology to human oncogene MCT-1; protein abundance increases in response to DNA replication stress
S000002958	YER060W-A	FCY22	FluoroCYtosine resistance	276572	278164	1	Putative purine-cytosine permease	Putative purine-cytosine permease; very similar to Fcy2p but cannot substitute for its function
S000002959	YER066C-A	""	""	291703	292203	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YER067W
S000002960	YER093C-A	AIM11	Altered Inheritance rate of Mitochondria	347912	348400	-1	Protein of unknown function	Protein of unknown function; null mutant is viable but shows increased loss of mitochondrial genome and synthetic interaction with prohibitin (phb1); contains an intron; SWAT-GFP and mCherry fusion proteins localize to the mitochondria; localizes to the peroxisome in glucose; YER093C-A has a paralog, YBL059W, that arose from the whole genome duplication
S000002961	YER119C-A	""	""	400867	401238	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2
S000002968	YFR024C-A	LSB3	Las Seventeen Binding protein	201960	203433	-1	Protein containing a C-terminal SH3 domain	Protein containing a C-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein abundance increases in response to DNA replication stress; LSB3 has a paralog, YSC84, that arose from the whole genome duplication
S000002969	YGL001C	ERG26	ERGosterol biosynthesis	495453	496502	-1	C-3 sterol dehydrogenase	C-3 sterol dehydrogenase; catalyzes the second of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; human homolog NSDHL implicated in CK syndrome, and can complement yeast null mutant; molecular target of natural product and antifungal compound FR171456
S000002970	YGL002W	ERP6	Emp24p/Erv25p Related Protein	494517	495167	1	Member of the p24 family involved in ER to Golgi transport	Member of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; ERP6 has a paralog, ERP1, that arose from the whole genome duplication
S000002971	YGL003C	CDH1	CDC20 Homolog	492474	494174	-1	Activator of anaphase-promoting complex/cyclosome (APC/C)	Activator of anaphase-promoting complex/cyclosome (APC/C); antagonist of the spindle assembly checkpoint; directs ubiquitination of cyclins resulting in mitotic exit; targets the APC/C to specific substrates including: Cdc20p, Ase1p, Cin8p, Fin1p and Clb5p; partially active in metaphase, and fully active in anaphase; cell-cycle regulated
S000002972	YGL004C	RPN14	Regulatory Particle Non-ATPase	490703	491956	-1	Evolutionarily conserved 19S regulatory particle assembly-chaperone	Evolutionarily conserved 19S regulatory particle assembly-chaperone; proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasome regulatory particle (RP); null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; interacts with Rpt5p
S000002973	YGL005C	COG7	Conserved Oligomeric Golgi complex	489708	490547	-1	Component of the conserved oligomeric Golgi complex	Component of the conserved oligomeric Golgi complex; a cytosolic tethering complex (Cog1p through Cog8p) that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
S000002974	YGL006W	PMC1	Plasma Membrane Calcium	485921	489442	1	Vacuolar Ca2+ ATPase involved in depleting cytosol of Ca2+ ions	Vacuolar Ca2+ ATPase involved in depleting cytosol of Ca2+ ions; prevents growth inhibition by activation of calcineurin in presence of elevated concentrations of calcium; targeted to vacuole via AP-3 pathway; similar to mammalian PMCA1a
S000002975	YGL007W	BRP1	""	483260	483637	1	Putative protein of unknown function	Putative protein of unknown function; located in the upstream region of PMA1; conserved among S. cerevisiae strains; mediates the cycloheximide-induced degradation of ubiquitinated Agp2p, a regulator of high affinity polyamine and carnitine transport; null mutant is polyamine resistant due to downregulation of PMA1
S000002976	YGL008C	PMA1	Plasma Membrane ATPase	479910	482666	-1	Plasma membrane P2-type H+-ATPase	Plasma membrane P2-type H+-ATPase; pumps protons out of cell; major regulator of cytoplasmic pH and plasma membrane potential; long-lived hexameric protein asymmetrically distributed at plasma membrane between mother cells and buds; accumulates at high levels in mother cells during aging, buds emerge with very low levels of Pma1p, newborn cells have low levels of Pma1p; Hsp30p plays a role in Pma1p regulation; interactions with Std1p appear to propagate [GAR+]
S000002978	YGL010W	MPO1	Metabolism of PHS to Odd-numbered fatty acids	475545	476069	1	Dioxygenase that catalyzes alpha-oxygenation of 2-hydroxy fatty acids	Dioxygenase that catalyzes alpha-oxygenation of 2-hydroxy fatty acids; involved in metabolism of phytosphingosine; not an essential gene
S000002979	YGL011C	SCL1	Suppressor of Crl3 ts Lethality	474489	475247	-1	Alpha 1 subunit of the 20S proteasome	Alpha 1 subunit of the 20S proteasome; involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria
S000002980	YGL012W	ERG4	ERGosterol biosynthesis	472855	474276	1	C-24(28) sterol reductase	C-24(28) sterol reductase; catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol
S000002981	YGL013C	PDR1	Pleiotropic Drug Resistance	469092	472298	-1	Transcription factor that regulates the pleiotropic drug response	Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; relocalizes to the cytosol in response to hypoxia; PDR1 has a paralog, PDR3, that arose from the whole genome duplication
S000002982	YGL014W	PUF4	PUmilio-homology domain Family	466141	468807	1	Member of the PUF protein family	Member of the PUF protein family; PUF family is defined by the presence of Pumilio homology domains that confer RNA binding activity; preferentially binds mRNAs encoding nucleolar ribosomal RNA-processing factors
S000002983	YGL015C	BIL2	Bud6-Interacting Ligand	465043	465435	-1	Formin inhibitor	Formin inhibitor; negatively regulates Bnr1-mediated actin filament nucleation but not filament elongation; performs distinct but overlapping roles with Hof1p to control actin cable assembly and polarized secretion; bnr1 bil2 double mutants are inviable; localizes to the cytosol, secretory vesicles and sites of polarized growth
S000002984	YGL016W	KAP122	KAryoPherin	461666	464911	1	Karyopherin beta	Karyopherin beta; bidirectional nuclear transport receptor (biportin) that shuttles distinct sets of cargoes in opposite directions; responsible for import of Toa1p-Toa2p complex into nucleus; depletes eIF5A and eEF2 from cell nuclei; binds to nucleoporins Nup1p and Nup2p; may play a role in regulation of pleiotropic drug resistance
S000002985	YGL017W	ATE1	Arginyl-tRNA-protein transfErase	459854	461365	1	Arginyl-tRNA-protein transferase	Arginyl-tRNA-protein transferase; catalyzes post-translational conjugation of arginine to the amino termini of acceptor proteins which are then subject to degradation via the N-end rule pathway; may have a role in regulating stress response
S000002986	YGL018C	JAC1	J-type Accessory Chaperone	459110	459664	-1	Specialized J-protein that functions in Fe-S cluster biogenesis	Specialized J-protein that functions in Fe-S cluster biogenesis; functions with Hsp70 in Fe-S cluster biogenesis in mitochondria; involved in iron metabolism; contains a J domain typical to J-type chaperones; localizes to the mitochondrial matrix
S000002987	YGL019W	CKB1	Casein Kinase Beta subunit	458156	458992	1	Beta regulatory subunit of casein kinase 2 (CK2)	Beta regulatory subunit of casein kinase 2 (CK2); a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases
S000002988	YGL020C	GET1	Guided Entry of Tail-anchored proteins	457163	457870	-1	Subunit of the GET complex	Subunit of the GET complex; involved in insertion of tail-anchored proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for normal mitochondrial morphology and inheritance; Get1p and Get2p form an aqueous channel for protein translocation and insertion into the membrane
S000002989	YGL021W	ALK1	""	454785	457067	1	Atypical protein kinase	Atypical protein kinase; along with paralog, ALK2, required for proper spindle positioning and nuclear segregation following mitotic arrest, proper organization of formins and polarisome components in mitosis, and survival after spindle assembly checkpoint delay; regulates the morphogenesis checkpoint in response to polarity insults through inactivation of Mih1p; phosphorylated in response to DNA damage; similar to mammalian haspins
S000002990	YGL022W	STT3	STaurosporine and Temperature sensitive	452404	454560	1	Catalytic subunit of the oligosaccharyltransferase (OST) complex	Catalytic subunit of the oligosaccharyltransferase (OST) complex; the OST complex catalyzes asparagine-linked glycosylation of newly synthesized proteins in the ER lumen; involved in recognition of N-acetylglucosamine; forms a subcomplex with Ost3p and Ost4p
S000002991	YGL023C	PIB2	PtdIns(3)p-Binding	450197	452104	-1	Phosphatidylinositol 3-phosphate binding protein	Phosphatidylinositol 3-phosphate binding protein; formation of a distinct complex with TORC1, induced by glutamine in dose-dependent manner, results in TORC1 activation, suggesting dual roles as both a glutamine sensor and a direct TORC1 activator; involved in leucine- and glutamine-responsive TORC1 activation, independent of the Gtr/Ego pathway; related to human PLEKHF1, a lysosomal membrane permeabilization regulator; contains a PtdIns3P binding FYVE domain; similar to Fab1p and Vps27p
S000002992	YGL024W	""	""	449788	450123	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF PGD1/YGL025C
S000002993	YGL025C	PGD1	PolyGlutamine Domain	448764	449957	-1	Subunit of the RNA polymerase II mediator complex	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor
S000002994	YGL026C	TRP5	TRyPtophan	446412	448535	-1	Tryptophan synthase	Tryptophan synthase; catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis
S000002995	YGL027C	CWH41	Calcofluor White Hypersensitive	443642	446143	-1	Processing alpha glucosidase I	Processing alpha glucosidase I; ER type II integral membrane N-glycoprotein involved in assembly of cell wall beta 1,6 glucan and asparagine-linked protein glycosylation; also involved in ER protein quality control and sensing of ER stress
S000002996	YGL028C	SCW11	Soluble Cell Wall protein	441281	442909	-1	Cell wall protein with similarity to glucanases	Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on its regulation by Ste12p
S000002997	YGL029W	CGR1	Coiled-coil Growth-Regulated	440063	440425	1	Protein involved in nucleolar integrity and processing of pre-rRNA	Protein involved in nucleolar integrity and processing of pre-rRNA; has a role in processing rRNA for the 60S ribosome subunit; transcript is induced in response to cytotoxic stress but not genotoxic stress; relocalizes from nucleus to nucleolus upon DNA replication stress
S000002999	YGL031C	RPL24A	Ribosomal Protein of the Large subunit	437467	437934	-1	Ribosomal 60S subunit protein L24A	Ribosomal 60S subunit protein L24A; forms two bridges within ribosome, stimulates translation initiation and elongation; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24A has a paralog, RPL24B, that arose from the whole genome duplication
S000003000	YGL032C	AGA2	a-AGglutinin	436570	436833	-1	Adhesion subunit of a-agglutinin of a-cells	Adhesion subunit of a-agglutinin of a-cells; C-terminal sequence acts as a ligand for alpha-agglutinin (Sag1p) during agglutination, modified with O-linked oligomannosyl chains, linked to anchorage subunit Aga1p via two disulfide bonds
S000003001	YGL033W	HOP2	HOmologous Pairing	435625	436401	1	Meiosis-specific protein that localizes to chromosomes	Meiosis-specific protein that localizes to chromosomes; prevents synapsis between nonhomologous chromosomes and ensures synapsis between homologs; forms complex with Mnd1p to promote homolog pairing and meiotic double-strand break repair; heterodimer of Hop2p-Mnd1p stimulates the Dmc1p-mediated strand invasion
S000003002	YGL034C	""	""	433214	433579	-1	Putative protein of unknown function	Putative protein of unknown function; conserved among S. cerevisiae strains; YGL034C is not an essential gene
S000003003	YGL035C	MIG1	Multicopy Inhibitor of GAL gene expression	431548	433062	-1	Transcription factor involved in glucose repression	Transcription factor involved in glucose repression; sequence specific DNA binding protein containing two Cys2His2 zinc finger motifs; regulated by the SNF1 kinase and the GLC7 phosphatase; regulates filamentous growth along with Mig2p in response to glucose depletion; activated in stochastic pulses of nuclear localization, shuttling between cytosol and nucleus depending on external glucose levels and its phosphorylation state
S000003004	YGL036W	VIR1	budding yeast ortholog of VIRMA/Virilizer/VIR	428601	431330	1	Protein required for mRNA m6A methylation and meiosis	Protein required for mRNA m6A methylation and meiosis; subunit of the m6A methyltransferase (MIS) complex; required for the stability of MIS complex subunits and may therefore have a scaffolding function; non-essential gene required for pre-meiotic S-phase; may be required to initiate the meiotic program, for progression through meiotic divisions and for spore formation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of VIRMA/virilizer/VIR
S000003005	YGL037C	PNC1	Pyrazinamidase and NiCotinamidase	427297	427947	-1	Nicotinamidase that converts nicotinamide to nicotinic acid	Nicotinamidase that converts nicotinamide to nicotinic acid; part of the NAD(+) salvage pathway; required for life span extension by calorie restriction; lacks a peroxisomal targeting signal but is imported into peroxisomes via binding to Gpd1p; PNC1 expression responds to all known stimuli that extend replicative life span; protein increases in abundance and relative distribution to cytoplasmic foci decreases upon DNA replication stress
S000003006	YGL038C	OCH1	Outer CHain elongation	425362	426804	-1	Mannosyltransferase of the cis-Golgi apparatus	Mannosyltransferase of the cis-Golgi apparatus; initiates the polymannose outer chain elongation of N-linked oligosaccharides of glycoproteins
S000003007	YGL039W	""	""	423961	425007	1	Aldehyde reductase	Aldehyde reductase; reduces aliphatic aldehyde substrates using NADH as cofactor; shown to reduce carbonyl compounds to chiral alcohols
S000003008	YGL040C	HEM2	HEMe biosynthesis	419527	420555	-1	Aminolevulinate dehydratase	Aminolevulinate dehydratase; a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus; human homolog ALAD can complement yeast hem2 mutant
S000003009	YGL041C	""	""	419022	419225	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000003010	YGL042C	""	""	418288	418593	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene DST1
S000003011	YGL043W	DST1	DNA Strand Transfer	417483	418412	1	General transcription elongation factor TFIIS	General transcription elongation factor TFIIS; enables RNA polymerase II to read through blocks to elongation by stimulating cleavage of nascent transcripts stalled at transcription arrest sites; maintains RNAPII elongation activity on ribosomal protein genes during conditions of transcriptional stress
S000003012	YGL044C	RNA15	poly(A) mRNA metabolism	416146	417036	-1	Component of the cleavage and polyadenylation factor I (CF I)	Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping and maintenance of genome stability
S000003013	YGL045W	RIM8	Regulator of IME2	414102	415730	1	Protein involved in proteolytic activation of Rim101p	Protein involved in proteolytic activation of Rim101p; part of response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family
S000003014	YGL046W	""	""	414100	414888	1	Merged open reading frame	Merged open reading frame; does not encode a discrete protein; YGL046W was originally annotated as an independent ORF, but as a result of a sequence change, it was merged with an adjacent ORF into a single reading frame, designated YGL045W
S000003015	YGL047W	ALG13	Asparagine-Linked Glycosylation	411552	412160	1	Catalytic component of UDP-GlcNAc transferase	Catalytic component of UDP-GlcNAc transferase; required for the second step of dolichyl-linked oligosaccharide synthesis; anchored to the ER membrane via interaction with Alg14p; similar to bacterial and human glycosyltransferases; protein abundance increases in response to DNA replication stress; both human homologs ALG13 and ALG14 are required to complement yeast alg13 mutant
S000003016	YGL048C	RPT6	Regulatory Particle Triple-A protein, or Regulatory Particle Triphosphatase	410069	411286	-1	ATPase of the 19S regulatory particle of the 26S proteasome	ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress
S000003017	YGL049C	TIF4632	Translation Initiation Factor	406860	409604	-1	Translation initiation factor eIF4G and scaffold protein	Translation initiation factor eIF4G and scaffold protein; subunit of the mRNA cap-binding protein complex (eIF4F) with eIF4E (Cdc33p) and eIF4A (Tif1p/Tif2p); proposed to stimulate the ATPase and helicase activities of eIF4A synergistically with eIF4G based on sequence similarity with Tif4631p; proposed to be required with eIF4E for Ded1p-mediated stimulation of mRNA recruitment during 48S preinitiation complex (PIC) assembly; associated with Pab1p-mediated poly(A)-tail stimulated, translation
S000003018	YGL050W	TYW3	Trna-YW synthesizing protein	405776	406597	1	tRNA methyltransferase required for synthesis of wybutosine	tRNA methyltransferase required for synthesis of wybutosine; a modified guanosine found at the 3'-position adjacent to the anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions
S000003019	YGL051W	MST27	Multicopy suppressor of Sec Twenty-one	403687	404391	1	Putative integral membrane protein, involved in vesicle formation	Putative integral membrane protein, involved in vesicle formation; forms complex with Mst28p; member of DUP240 gene family; binds COPI and COPII vesicles; MST27 has a paralog, MST28, that arose from a segmental duplication
S000003021	YGL053W	PRM8	Pheromone-Regulated Membrane protein	402589	403302	1	Pheromone-regulated protein	Pheromone-regulated protein; contains with 2 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; forms a complex with Prp9p in the ER; member of DUP240 gene family; PRM8 has a paralog, PRM9, that arose from a segmental duplication
S000003022	YGL054C	ERV14	ER Vesicle	400871	401287	-1	COPII-coated vesicle protein	COPII-coated vesicle protein; involved in vesicle formation and incorporation of specific secretory cargo; required for the delivery of bud-site selection protein Axl2p and Nha1p antiporter to cell surface; related to Drosophila cornichon; ERV14 has a paralog, ERV15, that arose from the whole genome duplication
S000003023	YGL055W	OLE1	OLEic acid requiring	398628	400160	1	Delta(9) fatty acid desaturase	Delta(9) fatty acid desaturase; required for monounsaturated fatty acid synthesis and for normal distribution of mitochondria
S000003024	YGL056C	SDS23	homolog of S. pombe SDS23	396035	397618	-1	Protein involved in cell separation during budding	Protein involved in cell separation during budding; one of two S. cerevisiae homologs (Sds23p and Sds24p) of the S. pombe Sds23 protein, which is implicated in APC/cyclosome regulation; SDS23 has a paralog, SDS24, that arose from the whole genome duplication
S000003025	YGL057C	GEP7	GEnetic interactors of Prohibitins	394966	395829	-1	Protein of unknown function	Protein of unknown function; null mutant exhibits a respiratory growth defect and synthetic interactions with prohibitin (phb1) and gem1; authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
S000003026	YGL058W	RAD6	RADiation sensitive	393986	394504	1	Ubiquitin-conjugating enzyme (E2)	Ubiquitin-conjugating enzyme (E2); involved in postreplication repair as a heterodimer with Rad18p, regulation of K63 polyubiquitination in response to oxidative stress, DSBR and checkpoint control as a heterodimer with Bre1p, ubiquitin-mediated N-end rule protein degradation as a heterodimer with Ubr1p, ERAD with Ubr1p in the absence of canonical ER membrane ligases, and Rpn4p turnover as part of proteasome homeostasis, in complex with Ubr2p and Mub1p
S000003027	YGL059W	PKP2	Protein Kinase of PDH	392223	393698	1	Mitochondrial protein kinase	Mitochondrial protein kinase; negatively regulates activity of the pyruvate dehydrogenase complex by phosphorylating the ser-133 residue of the Pda1p subunit; acts in concert with kinase Pkp1p and phosphatases Ptc5p and Ptc6p; relocalizes from mitochondrion to cytoplasm upon DNA replication stress
S000003028	YGL060W	YBP2	Yap1-Binding Protein	390065	391990	1	Central kinetochore associated protein	Central kinetochore associated protein; mediates mitotic progression; interacts with several central kinetochore proteins and centromeric histone Cse4p; role in resistance to oxidative stress; similar to Slk19p; YBP2 has a paralog, YBP1, that arose from the whole genome duplication
S000003029	YGL061C	DUO1	Death Upon Overproduction	388968	389711	-1	Essential subunit of the Dam1 complex (aka DASH complex)	Essential subunit of the Dam1 complex (aka DASH complex); cooperates with Dam1p and Pse1p to connect the DASH complex with microtubules (MT); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; is transferred to the kinetochore prior to mitosis
S000003030	YGL062W	PYC1	PYruvate Carboxylase	385196	388732	1	Pyruvate carboxylase isoform	Pyruvate carboxylase isoform; cytoplasmic enzyme that converts pyruvate to oxaloacetate; differentially regulated than isoform Pyc2p; mutations in the human homolog are associated with lactic acidosis; PYC1 has a paralog, PYC2, that arose from the whole genome duplication
S000003031	YGL063W	PUS2	PseudoUridine Synthase	383654	384766	1	Mitochondrial tRNA:pseudouridine synthase	Mitochondrial tRNA:pseudouridine synthase; acts at positions 27 and 28, but not at position 72; efficiently and rapidly targeted to mitochondria, specifically dedicated to mitochondrial tRNA modification; mutation also affects pseudouridylation of some nuclear-encoded mRNAs; PUS2 has a paralog, PUS1, that arose from the whole genome duplication
S000003032	YGL064C	MRH4	Mitochondrial RNA Helicase	381523	383208	-1	Mitochondrial ATP-dependent RNA helicase of the DEAD-box family	Mitochondrial ATP-dependent RNA helicase of the DEAD-box family; required for assembly of the large subunit of mitochondrial ribosomes; binds to the large subunit rRNA, 21S_rRNA; localizes to the matrix face of the mitochondrial inner membrane and associates with the large subunit precursor and with mature ribosomes
S000003033	YGL065C	ALG2	Asparagine-Linked Glycosylation	379760	381271	-1	Mannosyltransferase in the N-linked glycosylation pathway	Mannosyltransferase in the N-linked glycosylation pathway; catalyzes two consecutive steps in N-linked glycosylation pathway; mutants exhibit temperature-sensitive growth and abnormal accumulation of lipid-linked oligosaccharide Man2GlcNAc2-PP-Dol; mutations of human ALG2 cause congenital myasthenic syndrome; human ALG2 complements temperature sensitivity and dolichol-linked oligosaccharide biosynthesis defect of alg2-1 mutant, but mutant form from a patient with CDG-Ii fails to complement
S000003034	YGL066W	SGF73	SaGa associated Factor, 73 kDa	377609	379582	1	Subunit of DUBm module of SAGA and SLIK	Subunit of DUBm module of SAGA and SLIK; has roles in anchoring deubiquitination module (DUBm) into SAGA and SLIK complexes, maintaining organization and ubiquitin-binding conformation of Ubp8p, thereby contributing to overall DUBm activity; involved in preinitiation complex assembly at promoters; relocalizes to cytosol under hypoxia; human homolog ATXN7 implicated in spinocerebellar ataxia, and can complement yeast null mutant
S000003035	YGL067W	NPY1	NADH PYrophosphatase	376101	377255	1	NADH diphosphatase (pyrophosphatase)	NADH diphosphatase (pyrophosphatase); hydrolyzes the pyrophosphate linkage in NADH and related nucleotides; localizes to peroxisomes; enzymatic component of one of several partly redundant surveillance pathways involved in decapping of non-canonical nicotinamide adenine dinucleotide (NAD) capped nuclear mRNAs (NAD-RNAs); also possesses deCoAping activity involved in the decapping of non-canonical dephospho-CoA capped RNA (dpCoA-RNA); nudix hydrolase family member
S000003036	YGL068W	MNP1	Mitochondrial-Nucleoid Protein	375087	375671	1	Mitochondrial ribosomal protein of the large subunit	Mitochondrial ribosomal protein of the large subunit; has similarity to E. coli L7/L12 and human MRPL7 ribosomal proteins; associates with the mitochondrial nucleoid; required for normal respiratory growth
S000003037	YGL069C	""	""	375050	375514	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 92% of ORF overlaps the uncharacterized ORF YGL068W; deletion in cyr1 mutant results in loss of stress resistance
S000003038	YGL070C	RPB9	RNA Polymerase B	374459	374827	-1	RNA polymerase II subunit B12.6	RNA polymerase II subunit B12.6; contacts DNA; mutations affect transcription start site selection and fidelity of transcription; positively regulates ATG1 transcription as a key regulator of autophagy
S000003039	YGL071W	AFT1	Activator of Ferrous Transport	372012	374084	1	Transcription factor involved in iron utilization and homeostasis	Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions localization is regulated by Grx3p, Grx4p, and Fra2p, and promoter binding is negatively regulated via Grx3p-Grx4p binding; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
S000003040	YGL072C	""	""	371003	371362	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HSF1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2
S000003041	YGL073W	HSF1	Heat Shock transcription Factor	368753	371254	1	Trimeric heat shock transcription factor	Trimeric heat shock transcription factor; activates multiple genes in response to diverse stresses and transient intracellular acidification; recognizes variable heat shock elements (HSEs) consisting of inverted NGAAN repeats; monitors translational status through an RQC (Ribosomal Quality Control)-mediated translation-stress signal; involved in diauxic shift; role in preventing L-A mycovirus pathogenesis; human homolog HSF1 with linker region mutations can complement yeast hsf1 mutant
S000003042	YGL074C	""	""	368596	368922	-1	Dubious open reading frame unlikely to encode a functional protein	Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential HSF1 gene encoding heat shock transcription factor
S000003043	YGL075C	MPS2	MonoPolar Spindle	366925	368088	-1	Essential membrane protein localized at nuclear envelope and SPBs	Essential membrane protein localized at nuclear envelope and SPBs; required for insertion of the newly duplicated spindle pole body into the nuclear envelope; potentially phosphorylated by Cdc28p; MPS2 has a paralog, CSM4, that arose from the whole genome duplication
S000003044	YGL076C	RPL7A	Ribosomal Protein of the Large subunit	364335	365996	-1	Ribosomal 60S subunit protein L7A	Ribosomal 60S subunit protein L7A; required for processing of 27SA3 pre-rRNA to 27SB pre-rRNA during assembly of large ribosomal subunit; depletion leads to a turnover of pre-rRNA; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); binds to Domain II of 25S and 5.8S rRNAs; homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7A has a paralog, RPL7B, that arose from the whole genome duplication
S000003045	YGL077C	HNM1	Hyper-resistance to Nitrogen Mustard	362225	363916	-1	Plasma membrane transporter for choline, ethanolamine, and carnitine	Plasma membrane transporter for choline, ethanolamine, and carnitine; involved in the uptake of nitrogen mustard and the uptake of glycine betaine during hypersaline stress; co-regulated with phospholipid biosynthetic genes and negatively regulated by choline and myo-inositol
S000003046	YGL078C	DBP3	Dead Box Protein	360288	361859	-1	RNA-Dependent ATPase, member of DExD/H-box family	RNA-Dependent ATPase, member of DExD/H-box family; involved in cleavage of site A3 within the ITS1 spacer during rRNA processing; not essential for growth, but deletion causes severe slow-growth phenotype
S000003047	YGL079W	KXD1	KxDL homolog	359444	360100	1	Subunit of the BLOC-1 complex involved in endosomal maturation	Subunit of the BLOC-1 complex involved in endosomal maturation; null mutant is sensitive to drug inducing secretion of vacuolar cargo; GFP-fusion protein localizes to the endosome
S000003048	YGL080W	MPC1	Mitochondrial Pyruvate Carrier	358636	359028	1	Highly conserved subunit of mitochondrial pyruvate carrier (MPC)	Highly conserved subunit of mitochondrial pyruvate carrier (MPC); MPC is a mitochondrial inner membrane complex that mediates pyruvate uptake and comprises Mpc1p and Mpc2p during fermentative growth, or Mcp1p and Mpc3p during respiratory growth; null mutant displays slow growth that is complemented by expression of human or mouse ortholog; mutation in human ortholog MPC1 is associated with lactic acidosis and hyperpyruvatemia
S000003049	YGL081W	""	""	357377	358339	1	Putative protein of unknown function	Putative protein of unknown function; non-essential gene; interacts genetically with CHS5, a gene involved in chitin biosynthesis
S000003050	YGL082W	""	""	355827	356972	1	Putative protein of unknown function	Putative protein of unknown function; MINDY family deubiquitinase that does not appear to contain catalytic activity; predicted prenylation/proteolysis target of Afc1p and Rce1p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; not an essential gene; YGL082W has a paralog, YPL191C, that arose from the whole genome duplication; ortholog of human MINDY1/FAM63A
S000003051	YGL083W	SCY1	""	353058	355472	1	Putative kinase	Putative kinase; suppressor of GTPase mutant; similar to bovine rhodopsin kinase; may have a role in intracellular sterol transport
S000003052	YGL084C	GUP1	Glycerol UPtake	350616	352298	-1	Plasma membrane protein involved in remodeling GPI anchors	Plasma membrane protein involved in remodeling GPI anchors; member of the MBOAT family of putative membrane-bound O-acyltransferases; role in misfolded protein quality control; proposed to be involved in glycerol transport; homolog of the mammalian Hedgehog pathway modulator HHATL; GUP1 has a paralog, GUP2, that arose from the whole genome duplication
S000003053	YGL085W	LCL3	Long Chronological Lifespan	349616	350440	1	Putative protein of unknown function	Putative protein of unknown function; mutant has long chronological lifespan; has homology to Staphylococcus aureus nuclease; GFP-fusion protein localizes to mitochondria; is induced in response to the DNA-damaging agent MMS
S000003054	YGL086W	MAD1	Mitotic Arrest-Deficient	347119	349368	1	Coiled-coil protein involved in spindle-assembly checkpoint	Coiled-coil protein involved in spindle-assembly checkpoint; required for inhibition of karyopherin/importin Pse1p (aka Kap121p) upon spindle assembly checkpoint arrest; phosphorylated by Mps1p upon checkpoint activation which leads to inhibition of anaphase promoting complex activity; forms a complex with Mad2p; gene dosage imbalance between MAD1 and MAD2 leads to chromosome instability
S000003055	YGL087C	MMS2	Methyl MethaneSulfonate sensitivity	346406	346904	-1	Ubiquitin-conjugating enzyme variant	Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress
S000003056	YGL088W	""	""	345836	346201	1	Putative protein of unknown function	Putative protein of unknown function; conserved across S. cerevisiae strains; partially overlaps snR10, a snoRNA required for preRNA processing
S000003057	YGL089C	MF(ALPHA)2	Mating Factor ALPHA	344791	345153	-1	Mating pheromone alpha-factor, made by alpha cells	Mating pheromone alpha-factor, made by alpha cells; interacts with mating type a cells to induce cell cycle arrest and other responses leading to mating; also encoded by MF(ALPHA)1, which is more highly expressed; binds copper(II) ions
S000003058	YGL090W	LIF1	Ligase Interacting Factor	343319	344584	1	Component of the DNA ligase IV complex	Component of the DNA ligase IV complex; this complex mediates nonhomologous end joining in DNA double-strand break repair; sumoylated by Siz1p and Nfi1p SUMO ligases on Lys301; physically interacts with Dnl4p and Nej1p; homologous to mammalian XRCC4 protein
S000003059	YGL091C	NBP35	Nucleotide Binding Protein	342056	343042	-1	Essential cytoplasmic iron-sulfur cluster binding protein	Essential cytoplasmic iron-sulfur cluster binding protein; forms a complex with Cfd1p that is involved in iron-sulfur protein assembly in the cytosol; similar to P-loop NTPases
S000003060	YGL092W	NUP145	NUclear Pore	337906	341859	1	Essential protein with distinct roles in two nuclear pore subcomplexes	Essential protein with distinct roles in two nuclear pore subcomplexes; catalyzes its own proteolytic cleavage in vivo to generate a C-terminal fragment that is a structural component of the Nup84p subcomplex (with roles in NPC biogenesis and localization of genes to the nuclear periphery), and an N-terminal fragment that is one of several FG-nucleoporins within the NPC central core directly responsible for nucleocytoplasmic transport; homologous to human NUP98
S000003062	YGL094C	PAN2	Poly(A)-binding protein-dependent poly(A) riboNuclease	331118	334465	-1	Catalytic subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex	Catalytic subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex; complex acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes
S000003063	YGL095C	VPS45	Vacuolar Protein Sorting	328874	330607	-1	Protein of the Sec1p/Munc-18 family	Protein of the Sec1p/Munc-18 family; essential for vacuolar protein sorting; required for the function of Pep12p and the early endosome/late Golgi SNARE Tlg2p; essential for fusion of Golgi-derived vesicles with the prevacuolar compartment; mutation in human VPS45 is associated with congenital neutropenia and primary myelofibrosis of infancy
S000003064	YGL096W	TOS8	Target Of Sbf	325331	326161	1	Homeodomain-containing protein and putative transcription factor	Homeodomain-containing protein and putative transcription factor; found associated with chromatin; target of SBF transcription factor; induced during meiosis and under cell-damaging conditions; TOS8 has a paralog, CUP9, that arose from the whole genome duplication
S000003065	YGL097W	SRM1	Suppressor of Receptor Mutations	321782	323230	1	Nucleotide exchange factor for Gsp1p	Nucleotide exchange factor for Gsp1p; localizes to the nucleus, required for nucleocytoplasmic trafficking of macromolecules; suppressor of the pheromone response pathway; potentially phosphorylated by Cdc28p; human homolog of the RAN GEF, RCC1, can complement a temperature sensitive point mutant
S000003066	YGL098W	USE1	Unconventional SNARE in the ER	317342	318079	1	Essential SNARE protein localized to the ER	Essential SNARE protein localized to the ER; involved in retrograde traffic from the Golgi to the ER and Sey1p-independent homotypic ER fusion; required for efficient nuclear fusion during mating; forms a complex with the SNAREs Sec22p, Sec20p and Ufe1p
S000003067	YGL099W	LSG1	Large-Subunit Gtpase	314631	316553	1	Putative GTPase involved in 60S ribosomal subunit biogenesis	Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
S000003068	YGL100W	SEH1	SEc13 Homolog	313234	314283	1	Subunit of the Nup84 nuclear pore and SEACAT subcomplexes	Subunit of the Nup84 nuclear pore and SEACAT subcomplexes; involved in nucleocytoplasmic transport and NPC biogenesis in the nuclear pore subcomplex; subunit of SEACAT, a subcomplex of the SEA complex that inhibits the TORC1 inhibitory role of SEACIT (Iml1p-Npr2p-Npr3p), a GAP for Gtr1p in response to amino acid limitation, thereby resulting in activation of TORC1 signaling; SEA is a coatomer-related complex that associates dynamically with the vacuole; human SEH1 homolog
S000003069	YGL101W	YGK1	""	312193	312840	1	5'-deoxynucleotidase	5'-deoxynucleotidase; non-essential gene involved in deoxyribonucleoside monophosphate degradation; interacts with the DNA helicase Hpr5p; YGL101W has a paralog, YBR242W, that arose from the whole genome duplication
S000003072	YGL104C	VPS73	Vacuolar Protein Sorting	308713	310173	-1	Mitochondrial protein	Mitochondrial protein; mutation affects vacuolar protein sorting; putative transporter; member of the sugar porter family; VPS73 has a paralog, YBR241C, that arose from the whole genome duplication
S000003073	YGL105W	ARC1	Aminoacyl-tRNA synthetase Cofactor	307437	308567	1	Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases	Protein that binds tRNA and methionyl- and glutamyl-tRNA synthetases; involved in tRNA delivery, stimulating catalysis, and ensuring localization; also binds quadruplex nucleic acids; protein abundance increases in response to DNA replication stress; methionyl-tRNA synthetase is Mes1p; glutamyl-tRNA synthetase is Gus1p
S000003075	YGL107C	RMD9	Required for Meiotic nuclear Division	304333	306273	-1	Mitochondrial protein that controls mitochondrial gene expression	Mitochondrial protein that controls mitochondrial gene expression; binds the dodecamer element in mitochondrial mRNAs, facilitates processing and delivery of mitochondrial mRNAs to ribosomes; located on matrix face of the inner membrane and loosely associated with mitoribosomes; RMD9 has a paralog, YBR238C, that arose from the whole genome duplication
S000003076	YGL108C	""	""	303649	304071	-1	Protein of unknown function, predicted to be palmitoylated	Protein of unknown function, predicted to be palmitoylated; SWAT-GFP, seamless-GFP and mCherry C-terminal fusion proteins localize to the cytosol, while N-terminal GFP fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress
S000003077	YGL109W	""	""	303513	303836	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the uncharacterized gene YGL108C
S000003078	YGL110C	CUE3	Coupling of Ubiquitin conjugation to ER degradation	301537	303411	-1	Subunit of ribosome-associated quality control trigger complex (RQT)	Subunit of ribosome-associated quality control trigger complex (RQT); has a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination
S000003082	YGL114W	""	""	293460	295637	1	Putative protein of unknown function	Putative protein of unknown function; predicted member of the oligopeptide transporter (OPT) family of membrane transporters
S000003083	YGL115W	SNF4	Sucrose NonFermenting	292033	293001	1	Activating gamma subunit of the AMP-activated Snf1p kinase complex	Activating gamma subunit of the AMP-activated Snf1p kinase complex; additional subunits of the complex are Snf1p and a Sip1p/Sip2p/Gal83p family member; activates glucose-repressed genes, represses glucose-induced genes; role in sporulation, and peroxisome biogenesis; protein abundance increases in response to DNA replication stress
S000003084	YGL116W	CDC20	Cell Division Cycle	289809	291641	1	Activator of anaphase-promoting complex/cyclosome (APC/C)	Activator of anaphase-promoting complex/cyclosome (APC/C); APC/C is required for metaphase/anaphase transition; directs ubiquitination of mitotic cyclins, Pds1p, and other anaphase inhibitors; cell-cycle regulated; potential Cdc28p substrate; relative distribution to the nucleus increases upon DNA replication stress
S000003085	YGL117W	ARO5	AROmatic amino acid requiring	288512	289309	1	Protein involved in aromatic amino acid biosynthesis	Protein involved in aromatic amino acid biosynthesis; up-regulated following GCN4 induction, amino acid starvation, rapamycin treatment in GCN4-dependent manner; promoter contains Gcn4 control response element, which is bound by Gcn4p in vivo
S000003086	YGL118C	""	""	288014	288451	-1	Putative protein of unknown function	Putative protein of unknown function; conserved among S. cerevisiae strains; YGL118C is not an essential gene
S000003087	YGL119W	COQ8	COenzyme Q	284442	285947	1	ATPase required for ubiquinone biosynthesis and respiratory growth	ATPase required for ubiquinone biosynthesis and respiratory growth; maintains levels of CoQ biosynthetic proteins; binds to CoQ biosynthesis intermediates; UbiB protein kinase-like family member that lacks canonical protein kinase activity; similar to prokaryotic proteins involved in ubiquinone biosynthesis; human homolog ADCK3 complements a coq8 null, is associated with CoQ and respiratory-chain deficiencies, and is mutated in autosomal-recessive cerebellar ataxia type 2
S000003088	YGL120C	PRP43	Pre-mRNA Processing	281634	283937	-1	RNA helicase in the DEAH-box family	RNA helicase in the DEAH-box family; functions in both RNA polymerase I and polymerase II transcript metabolism; catalyzes removal of U2, U5, and U6 snRNPs from the postsplicing lariat-intron ribonucleoprotein complex; required for efficient biogenesis of both small- and large-subunit rRNAs; acts with Sqs1p to promote 20S to 18S rRNA processing catalyzed by endonuclease Nob1p
S000003089	YGL121C	GPG1	G Protein Gamma	280777	281157	-1	Proposed gamma subunit of the heterotrimeric G protein	Proposed gamma subunit of the heterotrimeric G protein; interacts with the receptor Gpr1p; involved in regulation of pseudohyphal growth; requires Gpb1p or Gpb2p to interact with Gpa2p; overproduction causes prion curing
S000003091	YGL123W	RPS2	Ribosomal Protein of the Small subunit	277617	278381	1	Protein component of the small (40S) subunit	Protein component of the small (40S) subunit; essential for control of translational accuracy; phosphorylation by C-terminal domain kinase I (CTDK-I) enhances translational accuracy; methylated on one or more arginine residues by Hmt1p; required for release of methyltransferase Bud23p from pre-40S precursor; homologous to mammalian ribosomal protein S2 and bacterial S5
S000003092	YGL124C	MON1	MONensin sensitivity	274780	276714	-1	Subunit of heterodimeric guanine nucleotide exchange factor (GEF)	Subunit of heterodimeric guanine nucleotide exchange factor (GEF); subunit of Mon1-Ccz1 GEF complex which stimulates nucleotide exchange and activation of Ypt7p, a Rab family GTPase involved in membrane tethering and fusion events at late endosome and vacuole; GEF activity is stimulated by membrane association and anionic phospholipids; role in localizing Ypt7p to vacuolar membrane; required for autophagy, CVT pathway, and mitophagy; targeted to vacuole via AP-3 pathway
S000003093	YGL125W	MET13	METhionine requiring	272520	274322	1	Major isozyme of methylenetetrahydrofolate reductase	Major isozyme of methylenetetrahydrofolate reductase; catalyzes the reduction of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate in the methionine biosynthesis pathway
S000003094	YGL126W	SCS3	Suppressor of Choline Sensitivity	270997	272139	1	Protein required for normal ER membrane biosynthesis in response to ER	Protein required for normal ER membrane biosynthesis in response to ER stress; ortholog of the FIT family of proteins involved in triglyceride droplet biosynthesis; human homolog FIT2/FITM2, a fatty acyl-CoA diphosphatase, complements the yeast null mutant; disputed role in the synthesis of inositol phospholipids from inositol; required for inositol prototrophy
S000003095	YGL127C	SOH1	Suppressor Of Hpr1	270392	270775	-1	Subunit of the RNA polymerase II mediator complex	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; involved in telomere maintenance; conserved with other metazoan MED31 subunits
S000003097	YGL129C	RSM23	Ribosomal Small subunit of Mitochondria	267723	269075	-1	Mitochondrial ribosomal protein of the small subunit	Mitochondrial ribosomal protein of the small subunit; has similarity to mammalian apoptosis mediator proteins; null mutation prevents induction of apoptosis by overproduction of metacaspase Mca1p
S000003098	YGL130W	CEG1	Capping Enzyme Guanylyltransferase	266145	267524	1	Guanylyltransferase involved in mRNA 5' capping	Guanylyltransferase involved in mRNA 5' capping; subunit of mRNA capping enzyme, which is a heterotetramer composed of two molecules of Ceg1p and a homodimer of Cet1p, the mRNA 5'-triphosphatase subunit; nuclear import of Ceg1p requires interaction with Cet1p; mammalian capping enzyme is a single bifunctional polypeptide; human homolog RNGTT can complement yeast ceg1 null mutant
S000003099	YGL131C	SNT2	""	261648	265859	-1	Subunit of Snt2C complex, RING finger ubiquitin ligase (E3)	Subunit of Snt2C complex, RING finger ubiquitin ligase (E3); physically associates with Ecm5p and Rpd3p; along with Ecm5p, recruits Rpd3p to small number of promoters; colocalizes with Ecm5p, independently of Rpd3p, to promoters of stress response genes upon oxidative stress; involved in ubiquitination, degradation of excess histones; interacts with Ubc4p; role in regulating genes encoding amine transporters; relocalizes from nucleus to cytoplasm upon DNA replication stress
S000003100	YGL132W	""	""	261580	261915	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene SNT2/YGL131C
S000003101	YGL133W	ITC1	Imitation switch Two Complex	257707	261501	1	Subunit of ATP-dependent Isw2p-Itc1p chromatin remodeling complex	Subunit of ATP-dependent Isw2p-Itc1p chromatin remodeling complex; required for repression of a-specific genes, repression of early meiotic genes during mitotic growth, and repression of INO1; similar to mammalian Acf1p, the regulatory subunit of the mammalian ATP-utilizing chromatin assembly and modifying factor (ACF) complex; ITC1 has a paralog, YPL216W, that arose from the whole genome duplication
S000003102	YGL134W	PCL10	Pho85 CycLin	255663	256964	1	Pho85p cyclin	Pho85p cyclin; recruits, activates, and targets Pho85p cyclin-dependent protein kinase to its substrate; PCL10 has a paralog, PCL8, that arose from the whole genome duplication
S000003103	YGL135W	RPL1B	Ribosomal Protein of the Large subunit	254641	255294	1	Ribosomal 60S subunit protein L1B	Ribosomal 60S subunit protein L1B; N-terminally acetylated; homologous to mammalian ribosomal protein L10A and bacterial L1; RPL1B has a paralog, RPL1A, that arose from the whole genome duplication; rpl1a rpl1b double null mutation is lethal; ribosomes containing Rpl1b are more efficient in translation of respiration-related proteins
S000003104	YGL136C	MRM2	Mitochondrial rRNA Methyl transferase	252897	253859	-1	Mitochondrial 2' O-ribose methyltransferase	Mitochondrial 2' O-ribose methyltransferase; required for methylation of U(2791) in 21S rRNA; MRM2 deletion confers thermosensitive respiration and loss of mitochondrial DNA; has similarity to Spb1p and Trm7p, and to E. coli FtsJ/RrmJ
S000003105	YGL137W	SEC27	SECretory	249869	252738	1	Essential beta'-coat protein of the COPI coatomer	Essential beta'-coat protein of the COPI coatomer; involved in ER-to-Golgi and Golgi-to-ER transport; contains WD40 domains that mediate cargo selective interactions; 45% sequence identity to mammalian beta'-COP
S000003106	YGL138C	""	""	248494	249531	-1	Putative protein of unknown function	Putative protein of unknown function; has no significant sequence similarity to any known protein
S000003107	YGL139W	FLC3	FLavin Carrier	245716	248124	1	Putative FAD transporter, similar to Flc1p and Flc2p	Putative FAD transporter, similar to Flc1p and Flc2p; localized to the ER; FLC3 has a paralog, FLC1, that arose from the whole genome duplication
S000003108	YGL140C	""	""	241353	245012	-1	Putative protein of unknown function	Putative protein of unknown function; non-essential gene; contains multiple predicted transmembrane domains
S000003109	YGL141W	HUL5	Hect Ubiquitin Ligase	238353	241085	1	Multiubiquitin chain assembly factor (E4)	Multiubiquitin chain assembly factor (E4); proteasome processivity factor that elongates polyUb chains on substrates, opposing Ubp6p, a branched polyubiquitin protease; required for retrograde transport of misfolded proteins during ERAD; required for ubiquitination of a subset of cytosolic misfolded proteins upon heat shock
S000003110	YGL142C	GPI10	GlycosylPhosphatidylInositol anchor biosynthesis	236269	238119	-1	Integral membrane protein involved in GPI anchor synthesis	Integral membrane protein involved in GPI anchor synthesis; putative alpha 1,2 mannosyltransferase required for addition of the third mannose onto the glycosylphosphatidylinositol (GPI) core structure; human PIG-Bp is a functional homolog
S000003111	YGL143C	MRF1	Mitochondrial peptide chain Release Factor	234717	235958	-1	Mitochondrial translation release factor RF1	Mitochondrial translation release factor RF1; involved in stop codon recognition and hydrolysis of peptidyl-tRNA bond during mitochondrial translation; lack of MRF1 causes mitochondrial genome instability
S000003112	YGL144C	ROG1	Revertant Of Glycogen synthase kinase mutation	232450	234507	-1	Lipase with specificity for monoacylglycerol	Lipase with specificity for monoacylglycerol; preferred substrate is 1-oleoylglycerol; null mutation affects lipid droplet morphology and overexpression causes increased accumulation of reactive oxygen species
S000003113	YGL145W	TIP20	SEC20 (Twenty) Interacting Protein	230243	232348	1	Peripheral membrane protein required for COPI vesicle fusion to the ER	Peripheral membrane protein required for COPI vesicle fusion to the ER; mediates Sey1p-independent homotypic ER fusion; prohibits back-fusion of COPII vesicles with the ER; forms a tethering complex with Sec39p and Dsl1p that interacts with ER SNAREs Sec20p and Use1p
S000003114	YGL146C	RRT6	Regulator of rDNA Transcription	228751	229686	-1	Integral membrane protein, member of the p24 family	Integral membrane protein, member of the p24 family; forms complex with Erp3p, Erp5p, and Emp24p; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription
S000003115	YGL147C	RPL9A	Ribosomal Protein of the Large subunit	227754	228329	-1	Ribosomal 60S subunit protein L9A	Ribosomal 60S subunit protein L9A; homologous to mammalian ribosomal protein L9 and bacterial L6; RPL9A has a paralog, RPL9B, that arose from a single-locus duplication
S000003116	YGL148W	ARO2	AROmatic amino acid requiring	226399	227529	1	Bifunctional chorismate synthase and flavin reductase	Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress
S000003117	YGL149W	""	""	225572	225877	1	Putative protein of unknown function	Putative protein of unknown function; conserved among S. cerevisiae strains; YGL149W is not an essential gene
S000003119	YGL151W	NUT1	Negative regulation of URS Two	217524	220922	1	Component of the RNA polymerase II mediator complex	Component of the RNA polymerase II mediator complex; mediator is required for transcriptional activation and also has a role in basal transcription
S000003120	YGL152C	""	""	216692	217369	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF PEX14/YGL153W
S000003121	YGL153W	PEX14	PEroXisome related	216273	217298	1	Central component of the peroxisomal importomer complex	Central component of the peroxisomal importomer complex; peroxisomal protein import machinery docking complex component; interacts with both PTS1 (Pex5p) and PTS2 (Pex7p) peroxisomal matrix protein signal recognition factors and membrane receptor Pex13p
S000003122	YGL154C	LYS5	LYSine requiring	215278	216096	-1	Phosphopantetheinyl transferase involved in lysine biosynthesis	Phosphopantetheinyl transferase involved in lysine biosynthesis; converts inactive apo-form of Lys2p (alpha-aminoadipate reductase) into catalytically active holo-form by posttranslational addition of phosphopantetheine
S000003124	YGL156W	AMS1	Alpha-MannoSidase	210416	213667	1	Vacuolar alpha mannosidase	Vacuolar alpha mannosidase; involved in free oligosaccharide (fOS) degradation; upregulated under glucose starvation; targeted to vacuole via CVT pathway and AP-3 pathway
S000003125	YGL157W	ARI1	Aldehyde Reductase Intermediate, subclass of SDR	209006	210049	1	NADPH-dependent aldehyde reductase	NADPH-dependent aldehyde reductase; utilizes aromatic and alophatic aldehyde substrates; member of the short-chain dehydrogenase/reductase superfamily
S000003126	YGL158W	RCK1	Radiation sensitivity Complementing Kinase	207033	208571	1	Protein kinase involved in oxidative stress response	Protein kinase involved in oxidative stress response; promotes pseudohyphal growth via activation of Ubp3p phosphorylation; identified as suppressor of S. pombe cell cycle checkpoint mutations; RCK1 has a paralog, RCK2, that arose from the whole genome duplication
S000003127	YGL159W	""	""	202721	203833	1	Putative protein of unknown function	Putative protein of unknown function; deletion mutant has no detectable phenotype
S000003128	YGL160W	AIM14	Altered Inheritance rate of Mitochondria	200561	202273	1	NADPH oxidase localized to the perinuclear ER	NADPH oxidase localized to the perinuclear ER; produces superoxide from NADPH; overexpression causes MCA1 dependent apoptosis; likely involved in superoxide-mediated regulation of the actin cytoskeleton; member of a conserved superfamily of NADPH oxidases (NOX enzymes); has similarity to iron/copper reductases (FRE1-8), particularly Fre8p
S000003129	YGL161C	YIP5	Ypt-Interacting Protein	199210	200142	-1	Protein that interacts with Rab GTPases	Protein that interacts with Rab GTPases; localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport
S000003130	YGL162W	SUT1	Sterol UpTake	198138	199037	1	Zn(II)2Cys6 family transcription factor	Zn(II)2Cys6 family transcription factor; positively regulates sterol uptake genes under anaerobic conditions; involved in hypoxic gene expression; represses filamentation-inducing genes during vegetative growth; positively regulates mating with SUT2 by repressing expression of genes that act as mating inhibitors; repressed by STE12; relocalizes from the nucleus to the cytoplasm upon DNA replication stress; SUT1 has a paralog, SUT2, that arose from the whole genome duplication
S000003131	YGL163C	RAD54	RADiation sensitive	193707	196403	-1	DNA-dependent ATPase that stimulates strand exchange	DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family of DNA translocases; forms nuclear foci upon DNA replication stress
S000003132	YGL164C	YRB30	Yeast Ran Binder	191978	193300	-1	RanGTP-binding protein	RanGTP-binding protein; inhibits RanGAP1 (Rna1p)-mediated GTP hydrolysis of RanGTP (Gsp1p); shares similarity to proteins in other fungi but not in higher eukaryotes
S000003133	YGL165C	""	""	191398	191976	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CUP2/YGL166W
S000003134	YGL166W	CUP2	""	191129	191806	1	Copper-binding transcription factor	Copper-binding transcription factor; activates transcription of the metallothionein genes CUP1-1 and CUP1-2 in response to elevated copper concentrations; required for regulation of copper genes in response to DNA-damaging reagents; CUP2 has a paralog, HAA1, that arose from the whole genome duplication
S000003135	YGL167C	PMR1	Plasma Membrane ATPase Related	187616	190468	-1	High affinity Ca2+/Mn2+ P-type ATPase	High affinity Ca2+/Mn2+ P-type ATPase; required for Ca2+ and Mn2+ transport into Golgi; involved in Ca2+ dependent protein sorting, processing; D53A mutant (Mn2+ transporting) is rapamycin sensitive, Q783A mutant (Ca2+ transporting) is rapamycin resistant; Mn2+ transport into Golgi lumen required for rapamycin sensitivity; mutations in human homolog ATP2C1 cause acantholytic skin condition Hailey-Hailey disease; human ATP2C1 can complement yeast null mutant
S000003136	YGL168W	HUR1	HydroxyUrea Resistance	187464	187796	1	Protein with a role in DNA repair	Protein with a role in DNA repair; mutants show reduced efficiency of non-homologous end-joining repair
S000003137	YGL169W	SUA5	Suppressor of Upstream AUG	186059	187339	1	Protein involved in threonylcarbamoyl adenosine biosynthesis	Protein involved in threonylcarbamoyl adenosine biosynthesis; Sua5p and Qri7p are necessary and sufficient for RNA t6A modification in vitro; null mutant lacks N6-threonylcarbamoyl adenosine (t6A) modification in the anticodon loop of ANN-decoding tRNA; member of conserved YrdC/Sua5 family; binds single-stranded telomeric DNA and null mutant has abnormal telomere length
S000003138	YGL170C	SPO74	SPOrulation	184153	185394	-1	Component of the meiotic outer plaque of the spindle pole body	Component of the meiotic outer plaque of the spindle pole body; involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation
S000003139	YGL171W	ROK1	Rescuer Of Kem1	182390	184084	1	RNA-dependent ATPase	RNA-dependent ATPase; involved in pre-rRNA processing at sites A0, A1, and A2, and in control of cell cycle progression; contains two upstream open reading frames (uORFs) in 5' untranslated region which regulate translation
S000003141	YGL173C	XRN1	eXoRiboNuclease	175527	180113	-1	Evolutionarily-conserved 5'-3' exonuclease	Evolutionarily-conserved 5'-3' exonuclease; deNADding enzyme that modulates mitochondrial NAD-capped RNA; component of cytoplasmic P-bodies involved in mRNA decay; positively regulates transcription initiation and elongation; involved in microtubule-mediated processes, filamentous growth, ribosomal RNA maturation, telomere maintenance, and tRNA intron turnover; negative regulator of autophagy; activated by scavenger decapping enzyme Dcs1p; role in preventing L-A mycovirus pathogenesis
S000003142	YGL174W	BUD13	BUD site selection	174545	175345	1	Subunit of the RES complex	Subunit of the RES complex; RES complex is required for nuclear pre-mRNA retention and splicing; involved in bud-site selection; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern due to a specific defect in MATa1 pre-mRNA splicing which leads to haploid gene expression in diploids
S000003143	YGL175C	SAE2	Sporulation in the Absence of spo Eleven	173285	174322	-1	Endonuclease required for telomere elongation	Endonuclease required for telomere elongation; required for telomeric 5' C-rich strand resection; involved in ds-break repair and processing hairpin DNA structures with the MRX complex; function requires sumoylation and phosphorylation; exists as inactive oligomers that are transiently released into smaller active units by phosphorylation; DNA damage triggers Sae2p removal, so active Sae2p is present only transiently; sequence and functional similarity with human CtIP/RBBP8
S000003144	YGL176C	""	""	171415	173079	-1	Protein of unknown function	Protein of unknown function; contributes to high inhibitor stress tolerance; deletion mutant is viable 
S000003145	YGL177W	""	""	167559	167906	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000003146	YGL178W	MPT5	Multicopy suppressor of Pop Two	167352	170571	1	mRNA-binding protein of the PUF family	mRNA-binding protein of the PUF family; binds to specific mRNAs, often in the 3' UTR; has broad specificity and binds to more than 1000 mRNAs (16% of the transcriptome); recruits the CCR4-NOT deadenylase complex to mRNAs along with Dhh1p and Dcp1p to promote deadenylation, decapping, and decay; also interacts with the Caf20p translational initiation repressor, affecting its mRNA target specificity
S000003147	YGL179C	TOS3	Target Of Sbf	163409	165091	-1	Protein kinase	Protein kinase; related to and functionally redundant with Elm1p and Sak1p for the phosphorylation and activation of Snf1p; localizes to cytosol and peroxisomes; functionally orthologous to LKB1, a mammalian kinase associated with Peutz-Jeghers cancer-susceptibility syndrome; TOS3 has a paralog, SAK1, that arose from the whole genome duplication
S000003148	YGL180W	ATG1	AuTophaGy related	160065	162758	1	Protein serine/threonine kinase	Protein serine/threonine kinase; required for vesicle formation in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; structurally required for phagophore assembly site formation; forms a complex with Atg13p and Atg17p during autophagy; required for re-phosphorylation of Atg13p during termination of autophagy, following prolonged nitrogen starvation; essential for cell cycle progression from G2/M to G1 under nitrogen starvation
S000003149	YGL181W	GTS1	Glycine Threonine Serine repeat protein	157906	159096	1	Protein involved in Arf3p regulation and in transcription regulation	Protein involved in Arf3p regulation and in transcription regulation; localizes to the nucleus and to endocytic patches; contains an N-terminal Zn-finger and ArfGAP homology domain, a C-terminal glutamine-rich region, and a UBA (ubiquitin associated) domain; gts1 mutations affect budding, cell size, heat tolerance, sporulation, life span, ultradian rhythms, endocytosis; expression oscillates in a pattern similar to metabolic oscillations
S000003152	YGL184C	STR3	Sulfur TRansfer	154615	156012	-1	Peroxisomal cystathionine beta-lyase	Peroxisomal cystathionine beta-lyase; converts cystathionine into homocysteine; may be redox regulated by Gto1p; involved in the release of the aromatic thiol 3-mercaptohexanol during wine fermentation
S000003153	YGL185C	""	""	153167	154306	-1	Putative protein with sequence similar to hydroxyacid dehydrogenases	Putative protein with sequence similar to hydroxyacid dehydrogenases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
S000003154	YGL186C	TPN1	Transport of PyridoxiNe	151037	152776	-1	Plasma membrane pyridoxine (vitamin B6) transporter	Plasma membrane pyridoxine (vitamin B6) transporter; member of the purine-cytosine permease subfamily within the major facilitator superfamily; proton symporter with similarity to Fcy21p, Fcy2p, and Fcy22p
S000003158	YGL190C	CDC55	Cell Division Cycle	145809	147389	-1	Regulatory subunit B of protein phosphatase 2A (PP2A)	Regulatory subunit B of protein phosphatase 2A (PP2A); Zds1p/2p-dependent localization to cytoplasm promotes mitotic entry; localization to nucleus prevents mitotic exit; required for correct nuclear division, chromosome segregation during achiasmate meiosis; maintains nucleolar sequestration of Cdc14p in early meiosis; limits formation of PP2A-Rts1p holocomplexes to ensure timely dissolution of sister chromosome cohesion; controls phosphorylation and stability of Cln2p; homolog of mammalian B55
S000003159	YGL191W	COX13	Cytochrome c OXidase	144808	145197	1	Subunit VIa of cytochrome c oxidase	Subunit VIa of cytochrome c oxidase; present in a subclass of cytochrome c oxidase complexes that may have a role in mimimizing generation of reactive oxygen species; not essential for cytochrome c oxidase activity but may modulate activity in response to ATP; required for assembly of Rcf2p into cytochrome c oxidase - cytochrome bc1 supercomplexes
S000003160	YGL192W	IME4	Inducer of MEiosis	142246	144048	1	mRNA N6-adenosine methyltransferase	mRNA N6-adenosine methyltransferase; essential for meiosis in S288C but dispensable for meiosis in SK1, mediates N6-adenosine methylation of bulk mRNA during the induction of sporulation which includes the meiotic regulators IME1, IME2 and IME4 itself; repressed in haploids via production of antisense IME4 transcripts; transcribed in diploid cells where antisense transcription is repressed; orthologous to human METTL3 (MT-A70)
S000003162	YGL194C	HOS2	Hda One Similar	140368	141726	-1	Histone deacetylase and subunit of Set3 and Rpd3L complexes	Histone deacetylase and subunit of Set3 and Rpd3L complexes; required for gene activation via specific deacetylation of lysines in H3 and H4 histone tails; subunit of the Set3 complex, a meiotic-specific repressor of sporulation specific genes that contains deacetylase activity; co-localizes with Cmr1p in nuclear foci in response to DNA damage by MMS
S000003163	YGL195W	GCN1	General Control Nonderepressible	131525	139543	1	Positive regulator of the Gcn2p kinase activity	Positive regulator of the Gcn2p kinase activity; forms a complex with Gcn20p; proposed to stimulate Gcn2p activation by an uncharged tRNA; suppresses frameshifting at stalled ribosomes
S000003164	YGL196W	DSD1	""	129883	131169	1	D-serine dehydratase (aka D-serine ammonia-lyase)	D-serine dehydratase (aka D-serine ammonia-lyase); converts D-serine to pyruvate and ammonia by a reaction dependent on pyridoxal 5'-phosphate and zinc; may play a role in D-serine detoxification; L-serine is not a substrate
S000003165	YGL197W	MDS3	Mck1 Dosage Suppressor	124698	129161	1	Putative component of the TOR regulatory pathway	Putative component of the TOR regulatory pathway; negative regulator of early meiotic gene expression; required, with Pmd1p, for growth under alkaline conditions; has an N-terminal kelch-like domain; MDS3 has a paralog, PMD1, that arose from the whole genome duplication
S000003166	YGL198W	YIP4	Ypt-Interacting Protein	123591	124298	1	Protein that interacts with Rab GTPases	Protein that interacts with Rab GTPases; localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport
S000003167	YGL199C	""	""	123572	124042	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YIP4/YGL198W
S000003168	YGL200C	EMP24	EndoMembrane Protein	122694	123305	-1	Component of the p24 complex	Component of the p24 complex; role in misfolded protein quality control; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles
S000003169	YGL201C	MCM6	MiniChromosome Maintenance	117854	120907	-1	Protein involved in DNA replication	Protein involved in DNA replication; component of the Mcm2-7 hexameric helicase complex that binds chromatin as a part of the pre-replicative complex; forms a subcomplex with Mcm4p and Mcm7p
S000003170	YGL202W	ARO8	AROmatic amino acid requiring	116059	117561	1	Aromatic aminotransferase I	Aromatic aminotransferase I; expression is regulated by general control of amino acid biosynthesis; involved with Aro9p in production of kynurenic acid to detoxify excess tryptophan
S000003171	YGL203C	KEX1	Killer EXpression defective	112475	114664	-1	Cell death protease essential for hypochlorite-induced apoptosis	Cell death protease essential for hypochlorite-induced apoptosis; involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins
S000003173	YGL205W	POX1	""	108158	110404	1	Fatty-acyl coenzyme A oxidase	Fatty-acyl coenzyme A oxidase; involved in the fatty acid beta-oxidation pathway; localized to the peroxisomal matrix; component of a light sensing pathway, converting light into a hydrogen peroxide signal, modulating nucleo-cytoplasmic shuttling of stress-responsive transcription factor, Msn2p
S000003174	YGL206C	CHC1	Clathrin Heavy Chain	102543	107504	-1	Clathrin heavy chain	Clathrin heavy chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; the clathrin triskelion is a trimeric molecule composed of three heavy chains that radiate from a vertex and three light chains which bind noncovalently near the vertex of the triskelion; the light chain (CLC1) is thought to regulate function
S000003176	YGL208W	SIP2	SNF1-Interacting Protein	97338	98585	1	One of three alternate beta subunits of the Snf1 kinase complex	One of three alternate beta subunits of the Snf1 kinase complex; involved in the response to glucose starvation; null mutants exhibit accelerated aging; N-myristoylprotein localized to the cytoplasm and the plasma membrane; SIP2 has a paralog, GAL83, that arose from the whole genome duplication
S000003177	YGL209W	MIG2	Multicopy Inhibitor of GAL gene expression	95858	97006	1	Zinc finger transcriptional repressor	Zinc finger transcriptional repressor; cooperates with Mig1p in glucose-induced gene repression; under low glucose conditions relocalizes to mitochondrion, where it interacts with Ups1p, antagonizes mitochondrial fission factor Dnm1p, indicative of a role in mitochondrial fusion or regulating morphology; regulates filamentous growth in response to glucose depletion; activated in stochastic pulses of nuclear localization in response to low glucose
S000003178	YGL210W	YPT32	Yeast Protein Two	93792	94460	1	Rab family GTPase involved in the exocytic pathway	Rab family GTPase involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; protein abundance increases in response to DNA replication stress; YPT32 has a paralog, YPT31, that arose from the whole genome duplication
S000003179	YGL211W	NCS6	Needs Cla4 to Survive	92512	93591	1	Protein required for uridine thiolation of Gln, Lys, and Glu tRNAs	Protein required for uridine thiolation of Gln, Lys, and Glu tRNAs; required for the thiolation of uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae; localizes to cytosol and peroxisomes
S000003180	YGL212W	VAM7	VAcuolar Morphogenesis	91432	92382	1	Vacuolar SNARE protein	Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress
S000003181	YGL213C	SKI8	SuperKIller	90054	91247	-1	Ski complex component and WD-repeat protein	Ski complex component and WD-repeat protein; mediates 3'-5' RNA degradation by the cytoplasmic exosome; also required for meiotic double-strand break recombination; null mutants have superkiller phenotype
S000003182	YGL214W	""	""	90010	90495	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation phenotype is likely due to the overlapping verified ORF SKI8/YGL213C
S000003183	YGL215W	CLG1	Cyclin-Like Gene	87981	89339	1	Cyclin-like protein that interacts with Pho85p	Cyclin-like protein that interacts with Pho85p; has sequence similarity to G1 cyclins PCL1 and PCL2
S000003184	YGL216W	KIP3	KInesin related Protein	84885	87302	1	Kinesin-related antiparallel sliding motor protein	Kinesin-related antiparallel sliding motor protein; involved in mitotic spindle positioning; sliding activity promotes bipolar spindle assembly and maintenance of genome stability; inhibits spindle elongation, destabilizing late anaphase spindle microtubules that polymerize beyond the midzone
S000003185	YGL217C	""	""	84839	85180	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF KIP3/YGL216W
S000003186	YGL218W	""	""	83650	84300	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 93% of ORF overlaps the verified gene MDM34; deletion in cyr1 mutant results in loss of stress resistance
S000003187	YGL219C	MDM34	Mitochondrial Distribution and Morphology	82878	84257	-1	Mitochondrial component of the ERMES complex	Mitochondrial component of the ERMES complex; links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth; required for mitophagy; ERMES complex is often co-localized with peroxisomes and with concentrated areas of pyruvate dehydrogenase
S000003188	YGL220W	BOL2	BolA-like protein	82374	82736	1	Cytosolic protein involved in repression of iron regulon transcription	Cytosolic protein involved in repression of iron regulon transcription; forms an iron-independent complex with Fra1p, Grx3p, and Grx4p; null mutant fails to repress the iron regulon and is sensitive to nickel; sequence similarity to human BOLA family member, BOLA2
S000003189	YGL221C	NIF3	Ngg1p-Interacting Factor	81426	82292	-1	Protein of unknown function	Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
S000003190	YGL222C	EDC1	Enhancer of mRNA DeCapping	80659	81186	-1	RNA-binding protein that activates mRNA decapping directly	RNA-binding protein that activates mRNA decapping directly; binds to mRNA substrate and enhances activity of decapping proteins Dcp1p and Dcp2p; has a role in translation during heat stress; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress; EDC1 has a paralog, EDC2, that arose from the whole genome duplication
S000003191	YGL223C	COG1	Conserved Oligomeric Golgi complex	79112	80365	-1	Essential component of the conserved oligomeric Golgi complex	Essential component of the conserved oligomeric Golgi complex; a cytosolic tethering complex (Cog1p through Cog8p) that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
S000003192	YGL224C	SDT1	Suppressor of Disruption of TFIIS	78015	78857	-1	Pyrimidine nucleotidase	Pyrimidine nucleotidase; responsible for production of nicotinamide riboside and nicotinic acid riboside; overexpression suppresses the 6-AU sensitivity of transcription elongation factor S-II, as well as resistance to other pyrimidine derivatives; SDT1 has a paralog, PHM8, that arose from the whole genome duplication
S000003193	YGL225W	VRG4	Vandate Resistance Glycosylation	76894	77907	1	Golgi GDP-mannose transporter	Golgi GDP-mannose transporter; regulates Golgi function and glycosylation in Golgi; VRG4 has a paralog, HVG1, that arose from the whole genome duplication
S000003194	YGL226C-A	OST5	OligoSaccharylTransferase	72749	73158	-1	Zeta subunit of the oligosaccharyltransferase complex of the ER lumen	Zeta subunit of the oligosaccharyltransferase complex of the ER lumen; complex catalyzes asparagine-linked glycosylation of newly synthesized proteins
S000003195	YGL226W	MTC3	Maintenance of Telomere Capping	73340	73711	1	Protein of unknown function	Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; mtc3 is synthetically sick with cdc13-1
S000003196	YGL227W	VID30	Vacuolar Import and Degradation	69671	72547	1	Central component of GID Complex, involved in FBPase degradation	Central component of GID Complex, involved in FBPase degradation; interacts strongly with Gid8p to serve as a scaffold for other GID Complex subunits; contains SPRY domain and 3 domains that are also found in Gid8p - LisH, CTLH, and CRA; required for association of Vid vesicles and actin patches in vacuole import and degradation pathway; shifts the balance of nitrogen metabolism toward glutamate production; localizes to the nucleus and the cytoplasm
S000003197	YGL228W	SHE10	Sensitivity to High Expression	67598	69331	1	Protein involved in outer spore wall assembly	Protein involved in outer spore wall assembly; likely involved directly in dityrosine layer assembly; putative GPI-anchored protein; overexpression causes growth arrest;; SWAT-GFP, seamless-GFP and mCherry fusion proteins localize to the endoplasmic reticulum; SHE10 has a paralog, OSW7/YFR039C, that arose from the whole genome duplication; paralogs are redundant for spore wall dityrosine assembly
S000003198	YGL229C	SAP4	Sit4 Associated Protein	64503	66959	-1	Protein required for function of the Sit4p protein phosphatase	Protein required for function of the Sit4p protein phosphatase; member of a family of similar proteins that form complexes with Sit4p, including Sap155p, Sap185p, and Sap190p; SAP4 has a paralog, SAP155, that arose from the whole genome duplication
S000003199	YGL230C	""	""	63772	64215	-1	Putative protein of unknown function	Putative protein of unknown function; non-essential gene
S000003200	YGL231C	EMC4	ER Membrane protein Complex	63048	63620	-1	Member of conserved ER transmembrane complex	Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm ZK616.6/EMC-4 and fly CG11137; mutation is functionally complemented by human EMC4
S000003201	YGL232W	TAN1	Trna AcetylatioN	62075	63002	1	Adaptor in Kre33p-mediated N4-acetylcytidine modification of tRNAs	Adaptor in Kre33p-mediated N4-acetylcytidine modification of tRNAs; tRNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine (ac4C) in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress; contains a conserved THUMP domain, and is homologous to human THUMPD1
S000003202	YGL233W	SEC15	SECretory	59122	61854	1	Essential 113 kDa subunit of the exocyst complex	Essential 113 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; interacts with and functions as a downstream effector of active, GTP-bound Sec4p, a Rab family GTPase
S000003203	YGL234W	ADE57	ADEnine requiring	56482	58890	1	Bifunctional enzyme of 'de novo' purine nucleotide pathway	Bifunctional enzyme of 'de novo' purine nucleotide pathway; contains both phosphoribosylglycinamidine synthetase/glycinamide ribotide synthetase (EC 6.3.4.13) ["Ade5"] and phosphoribosylaminoimidazole synthetase/aminoimidazole ribotide synthetase (EC 6.3.3.1) ["Ade7"] activities
S000003204	YGL235W	""	""	55279	55815	1	Putative protein of unknown function	Putative protein of unknown function; potential Cdc28p substrate; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2
S000003205	YGL236C	MTO1	Mitochondrial Translation Optimization	53787	55796	-1	Mitochondrial protein	Mitochondrial protein; forms heterodimer complex with Mss1p that performs 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; required for respiration in paromomycin-resistant 15S rRNA mutants; human homolog MTO1 can complement yeast null mutant
S000003206	YGL237C	HAP2	Heme Activator Protein	52731	53528	-1	Subunit of the Hap2p/3p/4p/5p CCAAT-binding complex	Subunit of the Hap2p/3p/4p/5p CCAAT-binding complex; complex is heme-activated and glucose-repressed; complex is a transcriptional activator and global regulator of respiratory gene expression; contains sequences sufficient for both complex assembly and DNA binding; respiratory defect of the null mutant is functionally complemented by human NFYA
S000003207	YGL238W	CSE1	Chromosome SEgregation	49552	52434	1	Nuclear envelope protein that acts as a recycling factor	Nuclear envelope protein that acts as a recycling factor; mediates the nuclear export of Srp1p (importin alpha) back to the cytoplasm after its import substrates have been released into the nucleoplasm, thereby allowing the participation of Srp1p in multiple rounds of nuclear import; required for accurate chromosome segregation; homolog of metazoan CAS and human CSE1L, overexpression of which is implicated in cancer progression
S000003208	YGL239C	""	""	49431	49745	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified gene CSE1
S000003209	YGL240W	DOC1	Destruction Of Cyclin B	48613	49365	1	Processivity factor	Processivity factor; required for ubiquitination activity of anaphase promoting complex (APC), mediates the activity of APC by contributing to substrate recognition; involved in cyclin proteolysis; contains conserved DOC1 homology domain
S000003210	YGL241W	KAP114	KAryoPherin	45445	48459	1	Karyopherin, responsible for nuclear import of specific proteins	Karyopherin, responsible for nuclear import of specific proteins; cargoes include Spt15p, Sua7p, histones H2A and H2B, and Nap1p; amino terminus shows similarity to those of other importins, particularly Cse1p; localization is primarily nuclear; function is regulated by sumoylation; protein abundance increases in response to DNA replication stress
S000003211	YGL242C	ANK1	Ankyrin repeat protein 1	44652	45197	-1	Cytoplasmic ankyrin repeat-containing protein	Cytoplasmic ankyrin repeat-containing protein; co-purifies with Myo3p and Myo5p by large-scale affinity capture; localization of the long-tailed type I myosins is altered in the null mutant; N-terminally propionylated in vivo; contains three ankyrin repeats and an acidic tail; orthologous to human OSTF1
S000003212	YGL243W	TAD1	tRNA-specific Adenosine Deaminase	43307	44509	1	tRNA-specific adenosine deaminase	tRNA-specific adenosine deaminase; deaminates adenosine-37 to inosine in tRNA-Ala
S000003213	YGL244W	RTF1	Restores TBP Function	41498	43174	1	Subunit of RNAPII-associated chromatin remodeling Paf1 complex	Subunit of RNAPII-associated chromatin remodeling Paf1 complex; regulates gene expression by directing cotranscriptional histone modification, influences transcription and chromatin structure through several independent functional domains; directly or indirectly regulates DNA-binding properties of Spt15p and relative activities of different TATA elements; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay
S000003214	YGL245W	GUS1	GlUtamyl-tRNA Synthetase	39023	41149	1	Glutamyl-tRNA synthetase (GluRS)	Glutamyl-tRNA synthetase (GluRS); forms a complex with methionyl-tRNA synthetase (Mes1p) and Arc1p; complex formation increases the catalytic efficiency of both tRNA synthetases and ensures their correct localization to the cytoplasm; protein abundance increases in response to DNA replication stress
S000003215	YGL246C	RAI1	Rat1p Interacting Protein	37617	38780	-1	Nuclear decapping endonuclease	Nuclear decapping endonuclease; targets mRNAs with unmethylated 7-methylguanosine cap structures and 5'-triphosphates; binds to and stabilizes the exoribonuclease Rat1p; required for pre-rRNA processing; involved in an early step of decapping non-canonical nicotinamide adenine dinucleotide (NAD) capped nuclear mRNAs (NAD-RNAs), part of ncRNA surveillance; relocalizes to the cytosol in response to hypoxia; homologous to human DOM3Z
S000003217	YGL248W	PDE1	PhosphoDiEsterase	35653	36762	1	Low-affinity cyclic AMP phosphodiesterase	Low-affinity cyclic AMP phosphodiesterase; controls glucose and intracellular acidification-induced cAMP signaling, target of the cAMP-protein kinase A (PKA) pathway; glucose induces transcription and inhibits translation
S000003218	YGL249W	ZIP2	ZIPping up meiotic chromosomes	33098	35212	1	Meiosis-specific protein	Meiosis-specific protein; involved in normal synaptonemal complex formation and pairing between homologous chromosomes during meiosis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress
S000003219	YGL250W	RMR1	Reduced Meiotic Recombination	31910	32635	1	Protein required for meiotic recombination and gene conversion	Protein required for meiotic recombination and gene conversion; null mutant displays reduced PIS1 expression and growth defects on non-fermentable carbon sources and minimal media; GFP-fusion protein localizes to both cytoplasm and nucleus
S000003220	YGL251C	HFM1	Helicase Family Member	27921	31636	-1	Meiosis specific DNA helicase	Meiosis specific DNA helicase; involved in the conversion of double-stranded breaks to later recombination intermediates and in crossover control; catalyzes the unwinding of Holliday junctions; has ssDNA and dsDNA stimulated ATPase activity
S000003221	YGL252C	RTG2	ReTroGrade regulation	25718	27484	-1	Sensor of mitochondrial dysfunction	Sensor of mitochondrial dysfunction; regulates the subcellular location of Rtg1p and Rtg3p, transcriptional activators of the retrograde (RTG) and TOR pathways; Rtg2p is inhibited by the phosphorylated form of Mks1p
S000003222	YGL253W	HXK2	HeXoKinase	23935	25395	1	Hexokinase isoenzyme 2	Hexokinase isoenzyme 2; phosphorylates glucose in cytosol; predominant hexokinase during growth on glucose; represses expression of HXK1, GLK1, induces expression of its own gene; antiapoptotic; phosphorylation/dephosphorylation at Ser14 by kinase Snf1p, phosphatase Glc7p-Reg1p regulates nucleocytoplasmic shuttling of Hxk2p; functions downstream of Sit4p in control of cell cycle, mitochondrial function, oxidative stress resistance, chronological lifespan; has paralog HXK1
S000003223	YGL254W	FZF1	Five Zinc Fingers	22304	23203	1	Transcription factor involved in sulfite metabolism	Transcription factor involved in sulfite metabolism; activates transcription of SSU1, DDI2, DDI3; overexpression suppresses sulfite-sensitivity of many unrelated mutants due to hyperactivation of SSU1, contains five zinc fingers; protein abundance increases in response to DNA replication stress
S000003224	YGL255W	ZRT1	Zinc-Regulated Transporter	20978	22108	1	High-affinity zinc transporter of the plasma membrane	High-affinity zinc transporter of the plasma membrane; responsible for the majority of zinc uptake; transcription is induced under low-zinc conditions by the Zap1p transcription factor
S000003225	YGL256W	ADH4	Alcohol DeHydrogenase	15159	16307	1	Alcohol dehydrogenase isoenzyme type IV	Alcohol dehydrogenase isoenzyme type IV; dimeric enzyme demonstrated to be zinc-dependent despite sequence similarity to iron-activated alcohol dehydrogenases; transcription is induced in response to zinc deficiency
S000003226	YGL257C	MNT2	MaNnosylTransferase	12481	14157	-1	Mannosyltransferase	Mannosyltransferase; involved in adding the 4th and 5th mannose residues of O-linked glycans
S000003227	YGL258W	VEL1	VELum formation	11110	11730	1	Protein of unknown function	Protein of unknown function; highly induced in zinc-depleted conditions and has increased expression in NAP1 deletion mutants; VEL1 has a paralog, YOR387C, that arose from a single-locus duplication
S000003228	YGL259W	YPS5	YaPSin	8470	8967	1	Protein with similarity to GPI-anchored aspartic proteases	Protein with similarity to GPI-anchored aspartic proteases; such proteases are Yap1p and Yap3p; mCherry fusion protein localizes to the vacuole
S000003229	YGL260W	""	""	6860	7090	1	Putative protein of unknown function	Putative protein of unknown function; transcription is significantly increased in a NAP1 deletion background; deletion mutant has increased accumulation of nickel and selenium
S000003230	YGL261C	PAU11	seriPAUperin	6290	6652	-1	Putative protein of unknown function	Putative protein of unknown function; member of the seripauperin multigene family encoded mainly in subtelomeric regions; mRNA expression appears to be regulated by SUT1 and UPC2
S000003231	YGL262W	""	""	5312	5839	1	Putative protein of unknown function	Putative protein of unknown function; null mutant displays elevated sensitivity to expression of a mutant huntingtin fragment or of alpha-synuclein; YGL262W is not an essential gene
S000003232	YGL263W	COS12	COnserved Sequence	2790	3932	1	Endosomal protein involved in turnover of plasma membrane proteins	Endosomal protein involved in turnover of plasma membrane proteins; member of the DUP380 subfamily of conserved, often subtelomeric COS genes; required for the multivesicular vesicle body sorting pathway that internalizes plasma membrane proteins for degradation; Cos proteins provide ubiquitin in trans for nonubiquitinated cargo proteins
S000003233	YGR001C	EFM5	Elongation Factor Methyltransferase	497133	498034	-1	S-adenosylmethionine-dependent lysine methyltransferase	S-adenosylmethionine-dependent lysine methyltransferase; involved in the trimethylation of eEF1A (Tef1p/Tef2p) at lysine 79; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; required for replication of Brome mosaic virus in budding yeast; expresses a circular RNA; originally misclassified as a N-6-adenine specific DNA methyltransferase based on sequence similarity; both Efm5p and human ortholog N6AMT2 can methylate eEF1a from either species in vitro
S000003235	YGR003W	CUL3	CULlin	500132	502366	1	Ubiquitin-protein ligase	Ubiquitin-protein ligase; forms a complex with Elc1p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; cullin family member with similarity to Cdc53p and human CUL3
S000003236	YGR004W	PEX31	PEroXisome related	502938	504326	1	Peroxisomal integral membrane protein	Peroxisomal integral membrane protein; involved in negative regulation of peroxisome size; partially functionally redundant with Pex30p and Pex32p; probably acts at a step downstream of steps mediated by Pex28p and Pex29p; PEX31 has a paralog, PEX30, that arose from the whole genome duplication
S000003238	YGR006W	PRP18	Pre-mRNA Processing	506070	506825	1	Splicing factor and component of snRNP U5	Splicing factor and component of snRNP U5; factor involved in the positioning of the 3' splice site during the second catalytic step of splicing; interacts with Slu7p
S000003239	YGR007W	ECT1	Ethanolamine-phosphate CytidylylTransferase	506969	507940	1	Ethanolamine-phosphate cytidylyltransferase	Ethanolamine-phosphate cytidylyltransferase; catalyzes the second step of phosphatidylethanolamine biosynthesis; involved in the maintenance of plasma membrane; similar to mammalian CTP: phosphocholine cytidylyl-transferases; inability of the null mutant to synthesize phosphatidylethanolamine and phosphatidylcholine from ethanolamine is functionally complemented by human PCYT2
S000003240	YGR008C	STF2	STabilizing Factor	508110	508364	-1	Protein involved in resistance to desiccation stress	Protein involved in resistance to desiccation stress; Stf2p exhibits antioxidant properties, and its overexpression prevents ROS accumulation and apoptosis; binds to F0 sector of mitochondrial F1F0 ATPase in vitro and may modulate the inhibitory action of Inh1p and Stf1p; protein abundance increases in response to DNA replication stress; STF2 has a paralog, TMA10, that arose from the whole genome duplication
S000003242	YGR010W	NMA2	Nicotinamide Mononucleotide Adenylyltransferase	511545	512732	1	Nicotinic acid mononucleotide adenylyltransferase	Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in de novo and salvage synthesis of NAD(+); homolog of human NMNAT; NMA2 has a paralog, NMA1, that arose from the whole genome duplication
S000003243	YGR011W	""	""	512497	512823	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000003244	YGR012W	MCY1	putative Mitochondrial CYsteine synthase	513159	514340	1	Putative cysteine synthase	Putative cysteine synthase; localized to the mitochondrial outer membrane
S000003245	YGR013W	SNU71	Small NUclear ribonucleoprotein associated	514554	516416	1	Component of U1 snRNP required for mRNA splicing via spliceosome	Component of U1 snRNP required for mRNA splicing via spliceosome; yeast specific, no metazoan counterpart
S000003246	YGR014W	MSB2	Multicopy Suppression of a Budding defect	516943	520863	1	Mucin family member involved in various signaling pathways	Mucin family member involved in various signaling pathways; functions as osmosensor in the Sho1p-mediated HOG pathway; functions in Cdc42p- and MAP kinase-dependent filamentous growth signaling pathway; processed into secreted and cell-associated forms by aspartyl protease Yps1p; potential Cdc28p substrate
S000003247	YGR015C	EAT1	Ethanol AcetylTransferase	521090	522076	-1	Ethanol acetyltransferase	Ethanol acetyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion
S000003248	YGR016W	""	""	522261	522833	1	Putative protein of unknown function	Putative protein of unknown function
S000003249	YGR017W	""	""	523787	524680	1	Pyridoxamine-phosphate oxidase-related protein (PNPO-RP)	Pyridoxamine-phosphate oxidase-related protein (PNPO-RP); deletion causes significant increase in 4.5.6-NADH3
S000003250	YGR018C	""	""	524365	524694	-1	Protein of unknown function	Protein of unknown function; mRNA identified as translated by ribosome profiling data; partially overlaps the uncharacterized ORF YGR017W
S000003251	YGR019W	UGA1	Utilization of GAba	525229	526644	1	Gamma-aminobutyrate (GABA) transaminase	Gamma-aminobutyrate (GABA) transaminase; also known as 4-aminobutyrate aminotransferase; involved in the 4-aminobutyrate and glutamate degradation pathways; required for normal oxidative stress tolerance and nitrogen utilization; protein abundance increases in response to DNA replication stress
S000003252	YGR020C	VMA7	Vacuolar Membrane Atpase	526973	527329	-1	Subunit F of the V1 peripheral membrane domain of V-ATPase	Subunit F of the V1 peripheral membrane domain of V-ATPase; part of the electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane; the V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase) has eight subunits
S000003253	YGR021W	DPC29	Delta-Psi dependent mitochondrial import and Cleavage protein of ~29 kDa	527632	528504	1	General post-initiation mitochondrial translation factor	General post-initiation mitochondrial translation factor; peripherally associated with the matrix side of the mitochondrial inner membrane; interacts with mitoribosomal proteins in actively translating cells; human ortholog TACO1 complements the yeast null mutant
S000003254	YGR022C	""	""	529257	529586	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W
S000003255	YGR023W	MTL1	Mid-Two Like	529264	530919	1	Transmembrane sensor of the cell wall integrity pathway (CWI)	Transmembrane sensor of the cell wall integrity pathway (CWI); involved in cell integrity signaling and stress response during glucose starvation and oxidative stress; structurally and functionally similar to Mid2p; MTL1 has a paralog, MID2, that arose from the whole genome duplication
S000003257	YGR025W	""	""	532639	532941	1	Putative protein of unknown function	Putative protein of unknown function; conserved across S. cerevisiae strains
S000003258	YGR026W	""	""	532985	533821	1	Putative protein of unknown function	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
S000003259	YGR027C	RPS25A	Ribosomal Protein of the Small subunit	534132	534458	-1	Protein component of the small (40S) ribosomal subunit	Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S25, no bacterial homolog; RPS25A has a paralog, RPS25B, that arose from the whole genome duplication
S000003260	YGR028W	MSP1	Mitochondrial Sorting of Proteins	542203	543291	1	Highly-conserved N-terminally anchored AAA-ATPase	Highly-conserved N-terminally anchored AAA-ATPase; distributed in the mitochondrial outer membrane and peroxisomes; involved in mitochondrial protein sorting; functions as an extraction engine in local organelle surveillance to remove and initiate degradation of mistargeted proteins, ensuring fidelity of organelle-specific localization of tail-anchored proteins; contains an N-terminal transmembrane domain and C-terminal cytoplasmic ATPase domain
S000003262	YGR030C	POP6	Processing Of Precursor RNAs	545681	546157	-1	Subunit of RNase MRP, nuclear RNase P and telomerase	Subunit of RNase MRP, nuclear RNase P and telomerase; forms a soluble heterodimer with Pop7p that binds P3 domain of RNase MRP and RNase P RNAs; RNase MRP cleaves pre-rRNA, nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs, while telomerase replenishes telomeric DNA; relocalizes to the cytosol in response to hypoxia
S000003263	YGR031W	IMO32	Intermediate cleaved by Mitochondrial Octapeptidyl aminopeptidase	546441	547469	1	Conserved mitochondrial protein of unknown function	Conserved mitochondrial protein of unknown function; processed by both mitochondrial processing peptidase and mitochondrial octapeptidyl aminopeptidase; gene contains the nested antisense gene NAG1
S000003264	YGR032W	GSC2	Glucan Synthase of Cerevisiae	548264	553951	1	Catalytic subunit of 1,3-beta-glucan synthase	Catalytic subunit of 1,3-beta-glucan synthase; involved in formation of the inner layer of the spore wall; activity positively regulated by Rho1p and negatively by Smk1p; GSC2 has a paralog, FKS1, that arose from the whole genome duplication
S000003265	YGR033C	TIM21	Translocase of the Inner Mitochondrial membrane	554248	554967	-1	Nonessential component of the TIM23 complex	Nonessential component of the TIM23 complex; interacts with the Translocase of the Outer Mitochondrial membrane (TOM complex) and with respiratory enzymes; may regulate the Translocase of the Inner Mitochondrial membrane (TIM23 complex) activity
S000003266	YGR034W	RPL26B	Ribosomal Protein of the Large subunit	555812	556672	1	Ribosomal 60S subunit protein L26B	Ribosomal 60S subunit protein L26B; binds to 5.8S rRNA; non-essential even when paralog is also deleted; deletion has minimal affections on ribosome biosynthesis; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26B has a paralog, RPL26A, that arose from the whole genome duplication
S000003267	YGR035C	""	""	557072	557422	-1	Putative protein of unknown function, potential Cdc28p substrate	Putative protein of unknown function, potential Cdc28p substrate; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; YGR035C has a paralog, YLR346C, that arose from the whole genome duplication
S000003268	YGR036C	CAX4	CAlmodulin-dependent in cmd one two twenty-siX	558149	558868	-1	Dolichyl pyrophosphate (Dol-P-P) phosphatase	Dolichyl pyrophosphate (Dol-P-P) phosphatase; cleaves the anhydride linkage in Dol-P-P; involved in  synthesis of Dol-P-P-linked oligosaccharide intermediate required for protein N-glycosylation; has a luminally oriented active site in the ER; mutation affects vesicular transport, membrane and cell wall biogenesis and lipid homeostasis
S000003269	YGR037C	ACB1	Acyl-CoA-Binding	559731	559994	-1	Acyl-CoA-binding protein	Acyl-CoA-binding protein; transports newly synthesized acyl-CoA esters from fatty acid synthetase (Fas1p-Fas2p) to acyl-CoA-consuming processes; subject to starvation-induced, Grh1p-mediated unconventional secretion; protein abundance increases in response to DNA replication stress
S000003270	YGR038W	ORM1	""	560682	561350	1	Protein that mediates sphingolipid homeostasis	Protein that mediates sphingolipid homeostasis; evolutionarily conserved, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; ORM1 has a paralog, ORM2, that arose from the whole genome duplication
S000003271	YGR039W	""	""	574888	575199	1	Putative protein of unknown function	Putative protein of unknown function; conserved among S. cerevisiae strains; YGR039W is not an essential gene
S000003272	YGR040W	KSS1	Kinase Suppressor of Sst2 mutations	575398	576504	1	Mitogen-activated protein kinase (MAPK)	Mitogen-activated protein kinase (MAPK); involved in signal transduction pathways that control filamentous growth and pheromone response; regulates septum assembly, and may directly phosphorylate Bni4p; the KSS1 gene is nonfunctional in some S288C-related strains and functional in W303a strains
S000003273	YGR041W	BUD9	BUD site selection	577487	579130	1	Protein involved in bud-site selection	Protein involved in bud-site selection; mutant has increased aneuploidy tolerance; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the distal pole; BUD9 has a paralog, BUD8, that arose from the whole genome duplication
S000003274	YGR042W	MTE1	Mph1-associated TElomere maintenance protein	579476	580291	1	Protein of unknown function	Protein of unknown function; involved in maintenance of proper telomere length; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; forms nuclear foci upon DNA replication stress
S000003275	YGR043C	NQM1	Non-Quiescent Mutant	580435	581436	-1	Transaldolase of unknown function	Transaldolase of unknown function; transcription is repressed by Mot1p and induced by alpha-factor and during diauxic shift; NQM1 has a paralog, TAL1, that arose from the whole genome duplication
S000003276	YGR044C	RME1	Regulator of MEiosis	582990	583892	-1	Zinc finger protein involved in control of meiosis	Zinc finger protein involved in control of meiosis; prevents meiosis by repressing IME1 expression and promotes mitosis by activating CLN2 expression; directly repressed by a1-alpha2 regulator; mediates cell type control of sporulation; relocalizes from nucleus to cytoplasm upon DNA replication stress
S000003277	YGR045C	""	""	583934	584296	-1	Putative protein of unknown function	Putative protein of unknown function; conserved across S. cerevisiae strains
S000003278	YGR046W	TAM41	Translocator Assembly and Maintenance	584895	586052	1	Mitochondrial phosphatidate cytidylyltransferase (CDP-DAG synthase)	Mitochondrial phosphatidate cytidylyltransferase (CDP-DAG synthase); required for cardiolipin biosynthesis; viability of null mutant is strain-dependent; mRNA is targeted to the bud; mutant displays defect in mitochondrial protein import, likely due to altered membrane lipid composition
S000003280	YGR048W	UFD1	Ubiquitin Fusion Degradation protein	589826	590911	1	Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase	Substrate-recruiting cofactor of the Cdc48p-Npl4p-Ufd1p segregase; polyubiquitin binding protein that assists in the dislocation of misfolded, ERAD substrates that are subsequently delivered to the proteasome for degradation; involved in regulated destruction of ER membrane proteins such as HMG-CoA reductase (Hmg1/2p) and cytoplasmic proteins (Fbp1p); involved in mobilizing membrane bound transcription factors by regulated Ub/proteasome-dependent processing (RUP)
S000003281	YGR049W	SCM4	Suppressor of Cdc4 Mutation	591314	591877	1	Mitochondrial outer membrane protein of unknown function	Mitochondrial outer membrane protein of unknown function; predicted to have 4 transmembrane segments; import is mediated by Tom70p and Mim1p; interacts genetically with a cdc4 mutation; SCM4 has a paralog, ATG33, that arose from the whole genome duplication
S000003282	YGR050C	""	""	592086	592442	-1	Protein of unknown function	Protein of unknown function; mRNA identified as translated by ribosome profiling data
S000003283	YGR051C	""	""	592906	593229	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YGR051C is not an essential gene
S000003284	YGR052W	FMP48	Found in Mitochondrial Proteome	593596	594705	1	Putative protein of unknown function	Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; induced by treatment with 8-methoxypsoralen and UVA irradiation
S000003285	YGR053C	MCO32	Mitochondrial Class One protein of 32 kDa	594986	595837	-1	Putative protein of unknown function	Putative protein of unknown function
S000003286	YGR054W	""	""	596693	598621	1	Eukaryotic initiation factor eIF2A	Eukaryotic initiation factor eIF2A; associates specifically with both 40S subunits and 80 S ribosomes, and interacts genetically with both eIF5b and eIF4E; homologous to mammalian eIF2A
S000003287	YGR055W	MUP1	Methionine UPtake	599417	601141	1	High affinity methionine permease	High affinity methionine permease; integral membrane protein with 13 putative membrane-spanning regions; involved in both methionine and cysteine uptake; subject to transport-activity dependent, methionine-induced endocytosis, vacuolar delivery and degradation; starvation-induced endocytosis is transport-activity independent
S000003288	YGR056W	RSC1	Remodel the Structure of Chromatin	601661	604447	1	Component of the RSC chromatin remodeling complex	Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; contains two essential bromodomains, a bromo-adjacent homology (BAH) domain, and an AT hook; RSC1 has a paralog, RSC2, that arose from the whole genome duplication
S000003289	YGR057C	LST7	Lethal with Sec Thirteen	604773	605501	-1	Subunit of the Lst4p-Lst7p GTPase activating protein complex for Gtr2p	Subunit of the Lst4p-Lst7p GTPase activating protein complex for Gtr2p; stimulates the GTPase activity of Rag family GTPase Gtr2p, within the context of the Gtr1p-Gtr2p heterodimer, after amino acid stimulation; required for activation of TORC1 in response to amino acid stimulation; recruited to the vacuolar membrane during amino acid starvation and released from the membrane by TORC1; required for the transport of amino acid permease Gap1p from the Golgi to the cell surface
S000003290	YGR058W	PEF1	Penta-EF-Hand protein	606136	607143	1	Penta-EF-hand protein	Penta-EF-hand protein; required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly
S000003291	YGR059W	SPR3	SPorulation Regulated	607563	609101	1	Sporulation-specific homolog of the CDC3/10/11/12 family of genes	Sporulation-specific homolog of the CDC3/10/11/12 family of genes; septin protein involved in sporulation; regulated by ABFI; the yeast CDC3/10/11/12 family is a family of bud neck microfilament genes
S000003292	YGR060W	ERG25	ERGosterol biosynthesis	610564	611493	1	C-4 methyl sterol oxidase	C-4 methyl sterol oxidase; catalyzes the first of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants accumulate the sterol intermediate 4,4-dimethylzymosterol; human MSMO1 functionally complements the growth defect caused by repression of ERG25 expression
S000003293	YGR061C	ADE6	ADEnine requiring	611889	615965	-1	Formylglycinamidine-ribonucleotide (FGAM)-synthetase	Formylglycinamidine-ribonucleotide (FGAM)-synthetase; catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway
S000003294	YGR062C	COX18	Cytochrome c OXidase	616328	617278	-1	Protein required for membrane insertion of C-terminus of Cox2p	Protein required for membrane insertion of C-terminus of Cox2p; mitochondrial integral inner membrane protein; interacts genetically and physically with Mss2p and Pnt1p; similar to S. cerevisiae Oxa1, N. crassa Oxa2p, and E. coli YidC; respiratory defect of the null mutant is functionally complemented by human COX18 carrying the N-terminal 54 amino acids of S. cerevisiae Cox18p
S000003295	YGR063C	SPT4	SuPpressor of Ty's	617516	617824	-1	Spt4p/5p (DSIF) transcription elongation factor complex subunit	Spt4p/5p (DSIF) transcription elongation factor complex subunit; Spt4/5 binds ssRNA in a sequence-specific manner and along with RNAP I and II regulates transcriptional elongation, RNA processing, quality control, and transcription-coupled repair; localizes to kinetochores and heterochromatin, influencing chromosomal dynamics and silencing; required for transcription through long trinucleotide repeats in ORFs and non-protein coding regions; accelerates senescence through rDNA instability
S000003296	YGR064W	""	""	617621	617989	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SPT4/YGR063C
S000003297	YGR065C	VHT1	Vitamin H Transporter	618077	619858	-1	High-affinity plasma membrane H+-biotin (vitamin H) symporter	High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin
S000003298	YGR066C	GID10	Glucose Induced Degradation deficient	620921	621799	-1	Recognition component (N-recognin) of the Pro/N-degron pathway	Recognition component (N-recognin) of the Pro/N-degron pathway; recognizes and targets for degradation proteins with N-terminal degradation signals; expressed only under starvation or osmotic stress
S000003299	YGR067C	""	""	622372	624786	-1	Putative protein of unknown function	Putative protein of unknown function; contains a zinc finger motif similar to that of Adr1p
S000003300	YGR068C	ART5	Arrestin-Related Trafficking adaptors	625324	627084	-1	Protein proposed to regulate endocytosis of plasma membrane proteins	Protein proposed to regulate endocytosis of plasma membrane proteins; regulates by recruiting the ubiquitin ligase Rsp5p to its target in the plasma membrane; SWAT-GFP and mCherry fusion proteins localize to the cytosol
S000003301	YGR069W	""	""	627083	627418	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000003302	YGR070W	ROM1	RhO1 Multicopy suppressor	627806	631273	1	Guanine nucleotide exchange factor (GEF) for Rho1p	Guanine nucleotide exchange factor (GEF) for Rho1p; mutations are synthetically lethal with mutations in rom2, which also encodes a GEP; ROM1 has a paralog, ROM2, that arose from the whole genome duplication
S000003303	YGR071C	ENV11	late ENdosome and Vacuole interface function	631481	634063	-1	Protein proposed to be involved in vacuolar functions	Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and fragmented vacuoles; deletion mutant has increased glycogen accumulation and displays elongated buds; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; ENV11 has a paralog, VID22, that arose from the whole genome duplication
S000003304	YGR072W	UPF3	UP Frameshift	634304	635467	1	Component of the nonsense-mediated mRNA decay (NMD) pathway	Component of the nonsense-mediated mRNA decay (NMD) pathway; along with Nam7p and Nmd2p; involved in decay of mRNA containing nonsense codons; involved in telomere maintenance
S000003305	YGR073C	""	""	635615	635986	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; extensively overlaps essential SMD1 gene encoding a U6 snRNP protein
S000003306	YGR074W	SMD1	snRNA Sm binding site protein D1	635712	636152	1	Core Sm protein Sm D1	Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; relocalizes to the cytosol in response to hypoxia; homolog of human Sm D1; protein abundance increases in response to DNA replication stress
S000003307	YGR075C	PRP38	Pre-mRNA Processing	636147	636875	-1	Unique component of the U4/U6.U5 tri-snRNP particle	Unique component of the U4/U6.U5 tri-snRNP particle; tri-snRNP is required for conformational changes which result in the catalytic activation of the spliceosome; dispensable for spliceosome assembly
S000003308	YGR076C	MRPL25	Mitochondrial Ribosomal Protein, Large subunit	637104	637577	-1	Mitochondrial ribosomal protein of the large subunit	Mitochondrial ribosomal protein of the large subunit; mutation confers increased replicative lifespan
S000003309	YGR077C	PEX8	PEroXin	637744	639513	-1	Intraperoxisomal organizer of the peroxisomal import machinery	Intraperoxisomal organizer of the peroxisomal import machinery; organizes the formation of the importomer complex, bridging the docking complex with the RING finger complex; tightly associated with the lumenal face of the peroxisomal membrane; essential for peroxisome biogenesis; binds PTS1-signal receptor Pex5p, and PTS2-signal receptor Pex7p
S000003310	YGR078C	PAC10	Perish in the Absence of Cin8p	639772	640371	-1	Part of the heteromeric co-chaperone GimC/prefoldin complex	Part of the heteromeric co-chaperone GimC/prefoldin complex; complex promotes efficient protein folding
S000003311	YGR079W	""	""	640720	641832	1	Protein of unknown function	Protein of unknown function; YGR079W is not an essential gene
S000003312	YGR080W	TWF1	TWinFilin	642010	643008	1	Twinfilin	Twinfilin; highly conserved actin monomer-sequestering protein involved in regulation of the cortical actin cytoskeleton; coordinates actin filament severing and monomer sequestering at sites of rapid actin turnover; composed of two cofilin-like regions, stimulates actin depolymerization as does the mouse homolog, mTwf1
S000003313	YGR081C	SLX9	""	643148	643780	-1	Nuclear export receptor for exportin Crm1p	Nuclear export receptor for exportin Crm1p; required for pre-rRNA processing and nuclear export of pre-40S pre-ribosomal particles; associates with the 90S pre-ribosome and 43S small ribosomal subunit precursor; interacts with U3 snoRNA; binds G-quadruplex (G4) structures in DNA; deletion mutant has synthetic fitness defect with an sgs1 deletion mutant
S000003315	YGR083C	GCD2	General Control Derepressed	644860	646815	-1	Delta subunit of the translation initiation factor eIF2B	Delta subunit of the translation initiation factor eIF2B; the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
S000003316	YGR084C	MRP13	Mitochondrial Ribosomal Protein	647123	648142	-1	Mitochondrial ribosomal protein of the small subunit	Mitochondrial ribosomal protein of the small subunit
S000003317	YGR085C	RPL11B	Ribosomal Protein of the Large subunit	648383	648907	-1	Ribosomal 60S subunit protein L11B	Ribosomal 60S subunit protein L11B; expressed at half the level of Rpl11Ap; involved in ribosomal assembly; depletion causes degradation of 60S proteins and RNA; homologous to mammalian ribosomal protein L11 and bacterial L5; RPL11B has a paralog, RPL11A, that arose from the whole genome duplication
S000003318	YGR086C	PIL1	Phosphorylation Inhibited by Long chain bases	649598	650617	-1	Eisosome core component involved in endocytosis	Eisosome core component involved in endocytosis; mitochondria phosphoprotein that localizes to the outer membrane and is involved in maintaining mitochondrial morphology and mitophagy; phosphorylated (T233) upon Pkc1p hyperactivation in a Slt2p MAPK-dependent fashion; null mutant shows activation of Pkc1p/Ypk1p stress resistance pathways; BAR domain family member; protein increases in abundance and relocalizes from the plasma membrane to cytoplasm upon DNA replication stress
S000003319	YGR087C	PDC6	Pyruvate DeCarboxylase	651290	652981	-1	Minor isoform of pyruvate decarboxylase	Minor isoform of pyruvate decarboxylase; decarboxylates pyruvate to acetaldehyde, involved in amino acid catabolism; transcription is glucose- and ethanol-dependent, and is strongly induced during sulfur limitation
S000003320	YGR088W	CTT1	CaTalase T	654634	656322	1	Cytosolic catalase T	Cytosolic catalase T; has a role in protection from oxidative damage by hydrogen peroxide
S000003321	YGR089W	NNF2	""	656960	659770	1	Protein that exhibits physical and genetic interactions with Rpb8p	Protein that exhibits physical and genetic interactions with Rpb8p; Rpb8p is a subunit of RNA polymerases I, II, and III; computational analysis of large-scale protein-protein interaction data suggests a role in chromosome segregation
S000003322	YGR090W	UTP22	U Three Protein	662358	666071	1	Component of the small-subunit processome	Component of the small-subunit processome; required for nuclear export of tRNAs; forms a complex with Rrp7p; may facilitate binding of Utp8p to aminoacylated tRNAs and their delivery to Los1p for export; conserved from yeast to mammals
S000003323	YGR091W	PRP31	Pre-mRNA Processing	666341	667825	1	Splicing factor	Splicing factor; component of the U4/U6-U5 snRNP complex; homologous to human PRPF31 and used to study retinitis pigmentosa
S000003324	YGR092W	DBF2	DumbBell Former	668189	669907	1	Ser/Thr kinase involved in transcription and stress response	Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis; DBF2 has a paralog, DBF20, that arose from the whole genome duplication
S000003325	YGR093W	DRN1	Debranching enzyme-associated RiboNuclease	670388	671911	1	Splicing factor that modulates turnover of branched RNAs by Dbr1p	Splicing factor that modulates turnover of branched RNAs by Dbr1p; interacts with spliceosomal components and branched RNA splicing products; enhances Dbr1p debranching in vitro; conserved protein with domain organization identical from yeast to human; N-terminal homology to Dbr1p N-terminus, but Dbr1p catalytic residues not conserved; relocalizes to the cytosol in response to hypoxia
S000003326	YGR094W	VAS1	VAlyl-tRNA Synthetase	672186	675500	1	Mitochondrial and cytoplasmic valyl-tRNA synthetase	Mitochondrial and cytoplasmic valyl-tRNA synthetase; human homolog VARS2 implicated in mitochondrial diseases, can partially complement yeast null mutant
S000003327	YGR095C	RRP46	Ribosomal RNA Processing	675671	676342	-1	Exosome non-catalytic core component	Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp46p (EXOSC5)
S000003328	YGR096W	TPC1	Thiamine Pyrophosphate Carrier	676621	677565	1	Mitochondrial membrane transporter	Mitochondrial membrane transporter; mediates uptake of the essential cofactor thiamine pyrophosphate (ThPP) into mitochondria; expression appears to be regulated by carbon source; member of the mitochondrial carrier family
S000003329	YGR097W	ASK10	Activator of SKn7	678695	682135	1	Regulator of the Fps1p glycerol channel	Regulator of the Fps1p glycerol channel; under nonstress conditions, binds to Fps1p to positively regulate glycerol transport; under osmotic stress, multiple phosphorylation by Hog1p causes Ask10p to dissociate from Fps1p; forms homodimers and heterodimerizes with paralog Rgc1p; phosphorylated in response to oxidative stress; has a role in destruction of Ssn8p; associates with RNA polymerase II holoenzyme
S000003330	YGR098C	ESP1	Extra Spindle Pole bodies	682566	687458	-1	Separase/separin, a caspase-like cysteine protease	Separase/separin, a caspase-like cysteine protease; cleaves Mcd1p/Scc1p, a mitotic cohesin complex subunit, resulting in the dissociation of cohesin from chromatin and sister chromatid separation; cleaves meiotic-cohesin subunit Rec8p along chromosome arms in meiosis I and at centromeric sites during meiosis II; inhibits PP2A-Cdc55p to promote mitotic exit; inhibited by Pds1p (securin); relative distribution to the nucleus increases upon DNA replication stress
S000003331	YGR099W	TEL2	TELomere maintenance	687899	689965	1	Subunit of the ASTRA complex, involved in chromatin remodeling	Subunit of the ASTRA complex, involved in chromatin remodeling; subunit of the telomere cap complex DNA-binding protein specific to single-stranded yeast telomeric DNA repeats, required for telomere length regulation and telomere position effect; involved in the stability or biogenesis of PIKKs such as TORC1
S000003332	YGR100W	MDR1	Mac1-Dependent Regulator	690245	693097	1	Cytoplasmic GTPase-activating protein	Cytoplasmic GTPase-activating protein; activates Ypt/Rab transport GTPases Ypt6p, Ypt31p and Sec4p; involved in recycling of internalized proteins and regulation of Golgi secretory function
S000003333	YGR101W	PCP1	Processing of Cytochrome c Peroxidase	693363	694403	1	Mitochondrial serine protease	Mitochondrial serine protease; required for the processing of various mitochondrial proteins and maintenance of mitochondrial DNA and morphology; belongs to the rhomboid-GlpG superfamily of intramembrane peptidases
S000003334	YGR102C	GTF1	Glutaminyl Transamidase subunit F	694584	695135	-1	Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex	Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; transposon insertion mutant is salt sensitive and null mutant has growth defects; non-tagged protein is detected in purified mitochondria
S000003336	YGR104C	SRB5	Suppressor of RNA polymerase B	697445	698368	-1	Subunit of the RNA polymerase II mediator complex	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance
S000003337	YGR105W	VMA21	Vacuolar Membrane Atpase	698599	698832	1	Integral membrane protein required for V-ATPase function	Integral membrane protein required for V-ATPase function; not actual component of vacuolar H+-ATPase (V-ATPase) complex; diverged ortholog of human VMA21/XMEA (X-linked Myopathy with Excessive Autophagy); functions in assembly of V-ATPase; localizes to endoplasmic reticulum (ER)
S000003338	YGR106C	VOA1	V0 Assembly protein	698989	699786	-1	ER protein that functions in assembly of the V0 sector of V-ATPase	ER protein that functions in assembly of the V0 sector of V-ATPase; functions with other assembly factors; null mutation enhances the vacuolar ATPase (V-ATPase) deficiency of a vma21 mutant impaired in endoplasmic reticulum (ER) retrieval
S000003339	YGR107W	""	""	702667	703116	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000003340	YGR108W	CLB1	CycLin B	703636	705051	1	B-type cyclin involved in cell cycle progression	B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome; CLB1 has a paralog, CLB2, that arose from the whole genome duplication
S000003341	YGR109C	CLB6	CycLin B	705359	706501	-1	B-type cyclin involved in DNA replication during S phase	B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1; CLB6 has a paralog, CLB5, that arose from the whole genome duplication
S000003342	YGR110W	CLD1	CardioLipin-specific Deacylase	713709	715046	1	Mitochondrial cardiolipin-specific phospholipase	Mitochondrial cardiolipin-specific phospholipase; functions upstream of Taz1p to generate monolyso-cardiolipin; transcription increases upon genotoxic stress; involved in restricting Ty1 transposition; has homology to mammalian CGI-58
S000003343	YGR111W	""	""	715828	717030	1	Putative protein of unknown function	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
S000003344	YGR112W	SHY1	SURF Homolog of Yeast	717358	718527	1	Mitochondrial inner membrane protein required for complex IV assembly	Mitochondrial inner membrane protein required for complex IV assembly; associates with complex IV assembly intermediates and complex III/complex IV supercomplexes; similar to human SURF1 involved in Leigh Syndrome; complex IV is also known as cytochrome c oxidase; also localizes to the peroxisome
S000003345	YGR113W	DAM1	Duo1 And Mps1 interacting	718893	719924	1	Essential subunit of the Dam1 complex (aka DASH complex)	Essential subunit of the Dam1 complex (aka DASH complex); cooperates with Duo1p and Pse1p to connect the DASH complex with the microtubules (MT); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; Ipl1p target for regulating kinetochore-MT attachments
S000003346	YGR114C	""	""	720265	720654	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; open reading frame overlaps 5' end of the essential gene SPT6
S000003348	YGR116W	SPT6	SuPpressor of Ty's	720409	724764	1	Histone chaperone	Histone chaperone; regulates chromatin structure, transcription, mRNA 3'-end formation and genome stability; role in chromatin remodeling and nucleosome assembly; required for initiation of DNA replication, promoting origin licensing through MCM helicase loading; required for fidelity of promoter selection through maintenance of chromatin structure during transcription; required for H3K36 trimethylation but not dimethylation by Set2p
S000003349	YGR117C	""	""	725049	726479	-1	Putative protein of unknown function	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
S000003350	YGR118W	RPS23A	Ribosomal Protein of the Small subunit	726974	727730	1	Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit	Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit; required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23A has a paralog, RPS23B, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal
S000003352	YGR120C	COG2	Conserved Oligomeric Golgi complex	730033	730821	-1	Essential component of the conserved oligomeric Golgi complex	Essential component of the conserved oligomeric Golgi complex; a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments; the components of the Golgi complex are Gog1p through Cog8p
S000003353	YGR121C	MEP1	""	731449	732927	-1	Ammonium permease	Ammonium permease; belongs to Mep-Amt-Rh family of well-conserved ammonium (NH4+) transporters that includes the human Rh factors; expression is under the nitrogen catabolite repression regulation; activity regulated by TORC1 effectors, Npr1p and Par32p; human homolog RHCG complements yeast null mutant; mutations in human homolog RHCG implicated in metabolic acidosis; MEP1 has a paralog, MEP3, that arose from the whole genome duplication
S000003354	YGR122W	""	""	733935	735143	1	Protein that may be involved in pH regulation	Protein that may be involved in pH regulation; probable ortholog of A. nidulans PalC, which is involved in pH regulation and binds to the ESCRT-III complex; null mutant does not properly process Rim101p and has decreased resistance to rapamycin; GFP-fusion protein is cytoplasmic; relative distribution to cytoplasm increases upon DNA replication stress
S000003355	YGR123C	PPT1	Protein Phosphatase T	736662	738203	-1	Protein serine/threonine phosphatase	Protein serine/threonine phosphatase; regulates Hsp90 chaperone by affecting its ATPase and cochaperone binding activities; has similarity to human phosphatase PP5; present in both the nucleus and cytoplasm; expressed during logarithmic growth
S000003356	YGR124W	ASN2	ASparagiNe requiring	739944	741662	1	Asparagine synthetase	Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN2 has a paralog, ASN1, that arose from the whole genome duplication
S000003357	YGR125W	VSB1	Vacuolar Storage of Basic amino acids	742325	745435	1	Vacuolar membrane protein required for uptake and storage of arginine 	Vacuolar membrane protein required for uptake and storage of arginine in nitrogen-replete cells; nitrogen starvation appears to inhibit Vsb1-dependent activity, resulting in mobilization of stored vacuolar arginine to the cytosol; arginine uptake appears to be driven by a V-ATPase mediated H+ gradient; member of the APC (amino acid-polyamine-organocation) transporter superfamily
S000003358	YGR126W	""	""	745835	746527	1	Putative protein of unknown function	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS
S000003359	YGR127W	""	""	746798	747736	1	Protein involved in heme trafficking to the nucleus	Protein involved in heme trafficking to the nucleus; expression is regulated by Msn2p/Msn4p, indicating possible role in stress response; localizes to cytosol and peroxisomes
S000003361	YGR129W	SYF2	SYnthetic lethal with cdc40 (Forty)	750400	751047	1	Member of the NineTeen Complex (NTC)	Member of the NineTeen Complex (NTC); NTC contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; relocalizes to the cytosol in response to hypoxia; isy1 syf2 cells have defective spindles activiating cell cycle arrest
S000003362	YGR130C	""	""	751394	753844	-1	Component of the eisosome with unknown function	Component of the eisosome with unknown function; GFP-fusion protein localizes to the cytoplasm; specifically phosphorylated in vitro by mammalian diphosphoinositol pentakisphosphate (IP7)
S000003363	YGR131W	FHN1	Functional Homolog of Nce102	754726	755250	1	Protein of unknown function	Protein of unknown function; induced by ketoconazole; promoter region contains sterol regulatory element motif, which has been identified as a Upc2p-binding site; overexpression complements function of Nce102p in NCE102 deletion strain; FHN1 has a paralog, NCE102, that arose from the whole genome duplication
S000003364	YGR132C	PHB1	ProHiBitin	755589	756452	-1	Subunit of the prohibitin complex (Phb1p-Phb2p)	Subunit of the prohibitin complex (Phb1p-Phb2p); prohibitin is a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation; prohibitin deficiency induces a mitochondrial unfolded protein response (mtUPR)
S000003365	YGR133W	PEX4	PEroXin	756896	757447	1	Peroxisomal ubiquitin conjugating enzyme	Peroxisomal ubiquitin conjugating enzyme; required for peroxisomal matrix protein import and peroxisome biogenesis
S000003366	YGR134W	CAF130	CCR4 Associated Factor	757770	761138	1	Subunit of the CCR4-NOT transcriptional regulatory complex	Subunit of the CCR4-NOT transcriptional regulatory complex; CCR4-NOT complex is evolutionarily-conserved and involved in controlling mRNA initiation, elongation, and degradation
S000003367	YGR135W	PRE9	PRoteinase yscE	761392	762168	1	Alpha 3 subunit of the 20S proteasome	Alpha 3 subunit of the 20S proteasome; the only nonessential 20S subunit; may be replaced by the alpha 4 subunit (Pre6p) under stress conditions to create a more active proteasomal isoform
S000003368	YGR136W	LSB1	Las Seventeen Binding protein	762428	763153	1	Negative regulator of actin nucleation-promoting factor activity	Negative regulator of actin nucleation-promoting factor activity; interacts with Las17p, a homolog of human Wiskott-Aldrich Syndrome protein (WASP), via an N-terminal SH3 domain, and along with PIN3 cooperatively inhibits the nucleation of actin filaments; overexpression blocks receptor-mediated endocytosis; protein increases in abundance and forms nuclear foci in response to DNA replication stress; LSB1 has a paralog, PIN3, that arose from the whole genome duplication
S000003369	YGR137W	""	""	762888	763262	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000003370	YGR138C	TPO2	Transporter of POlyamines	763762	765606	-1	Polyamine transporter of the major facilitator superfamily	Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; specific for spermine; localizes to the plasma membrane; transcription of TPO2 is regulated by Haa1p; TPO2 has a paralog, TPO3, that arose from the whole genome duplication
S000003371	YGR139W	""	""	765726	766064	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000003372	YGR140W	CBF2	Centromere-Binding Factor	767429	770299	1	Essential kinetochore protein	Essential kinetochore protein; component of the CBF3 multisubunit complex that binds to the CDEIII region of the centromere; Cbf2p also binds to the CDEII region possibly forming a different multimeric complex, ubiquitinated in vivo; sumoylated in an Mms21p-dependent manner; relative distribution to the spindle pole body decreases upon DNA replication stress
S000003373	YGR141W	VPS62	Vacuolar Protein Sorting	770569	771972	1	Vacuolar protein sorting (VPS) protein	Vacuolar protein sorting (VPS) protein; required for cytoplasm to vacuole targeting of proteins; targeted to vacuole via AP-3 pathway; VPS62 has a paralog, TDA6, that arose from the whole genome duplication
S000003374	YGR142W	BTN2	BaTteN disease	772454	773686	1	v-SNARE binding protein	v-SNARE binding protein; facilitates specific protein retrieval from a late endosome to the Golgi; modulates arginine uptake, possible role in mediating pH homeostasis between the vacuole and plasma membrane H(+)-ATPase; contributes to prion curing; preferentially expressed after severe ethanol stress, involved in clearance of denatured proteins caused by severe ethanol stress
S000003375	YGR143W	SKN1	Suppressor of Kre Null	775193	777508	1	Protein involved in sphingolipid biosynthesis	Protein involved in sphingolipid biosynthesis; type II membrane protein; SKN1 has a paralog, KRE6, that arose from the whole genome duplication
S000003376	YGR144W	THI4	THIamine metabolism	780399	781379	1	Thiazole synthase	Thiazole synthase; abundant protein involved in the formation of the thiazole moiety of thiamine during thiamine biosynthesis; acts more as a co-substrate rather than an enzyme by providing the sulphur source for thiazole formation; undergoes a single turnover only; required for mitochondrial genome stability in response to DNA damaging agents
S000003377	YGR145W	ENP2	Essential Nuclear Protein	781767	783890	1	Component of the SSU	Component of the SSU; required for pre-18S rRNA processing, biogenesis of the small ribosomal subunit; interacts with U3 snoRNA, Mpp10p and Bfr2p; contains WD repeats, and has homology to Spb1p
S000003378	YGR146C	ECL1	Extends Chronological Lifespan	784223	784858	-1	Protein of unknown function	Protein of unknown function; mitochondrial-dependent role in the extension of chronological lifespan; overexpression increases oxygen consumption and respiratory activity while deletion results in reduced oxygen consumption under conditions of caloric restriction; induced by iron homeostasis transcription factor Aft2p; multicopy suppressor of temperature sensitive hsf1 mutant; induced by treatment with 8-methoxypsoralen and UVA irradiation
S000003379	YGR147C	NAT2	N-terminal AcetylTransferase	786054	786920	-1	Protein of unknown function	Protein of unknown function; has an apparent role in acetylation of N-terminal methionine residues
S000003380	YGR148C	RPL24B	Ribosomal Protein of the Large subunit	787312	787779	-1	Ribosomal 60S subunit protein L24B	Ribosomal 60S subunit protein L24B; forms two bridges within ribosome, stimulates translation initiation and elongation; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication
S000003381	YGR149W	GPC1	GlyceroPhosphoCholine acyltransferase	789031	790329	1	Glycerophosphocholine acyltransferase (GPCAT)	Glycerophosphocholine acyltransferase (GPCAT); involved in in phosphatidylcholine (PC) synthesis; uses acyl-CoA to acylate glycero-3-phosphocholine to yield lyso-PC; also catalyzes acylation of glycerophosphoethanolamine with acyl-CoA; predicted to be an integral membrane protein
S000003382	YGR150C	CCM1	COB and COX1 mRNA maturation	790459	793053	-1	Mitochondrial 15S rRNA-binding protein	Mitochondrial 15S rRNA-binding protein; required for intron removal of COB and COX1 pre-mRNAs; has separable roles in stabilizing mitochondrial 15S rRNA and in maturation of the COB and COX1 mRNAs; contains pentatricopeptide repeat (PPR) motifs; mutant is respiratory deficient and has defective plasma membrane electron transport
S000003383	YGR151C	""	""	794655	794990	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF RSR1/BUD1/YGR152C; relative distribution to the nucleus increases upon DNA replication stress
S000003384	YGR152C	RSR1	RaS-Related	794674	795492	-1	Ras-type GTPase	Ras-type GTPase; required for bud site selection during both axial and bipolar budding, for morphological changes in response to mating pheromone, and for efficient cell fusion; recruits Cdc24p to the incipient bud site, activating its guanyl-nucleotide exchange factor activity and the Rho-like GTPase, Cdc42p; localizes to the plasma membrane, the incipient bud site, polarized growth sites, the cell division site and the vacuole membrane; Ras superfamily member similar to mammalian Rap GTPases
S000003385	YGR153W	""	""	796092	796745	1	Putative protein of unknown function	Putative protein of unknown function
S000003386	YGR154C	GTO1	Glutathione Transferase Omega-like	796798	797868	-1	Omega-class glutathione transferase	Omega-class glutathione transferase; induced under oxidative stress; putative peroxisomal localization
S000003387	YGR155W	CYS4	CYStathionine beta-synthase	798543	800066	1	Cystathionine beta-synthase	Cystathionine beta-synthase; catalyzes synthesis of cystathionine from serine and homocysteine, the first committed step in cysteine biosynthesis; responsible for hydrogen sulfide generation; advances passage through START by promoting cell growth which requires catalytic activity, and reducing critical cell size independent of catalytic activity; mutations in human ortholog CBS cause homocystinuria; human CBS can complement yeast null mutant
S000003389	YGR157W	CHO2	CHOline requiring	802440	805049	1	Phosphatidylethanolamine methyltransferase (PEMT)	Phosphatidylethanolamine methyltransferase (PEMT); catalyzes the first step in the conversion of phosphatidylethanolamine to phosphatidylcholine during the methylation pathway of phosphatidylcholine biosynthesis
S000003390	YGR158C	MTR3	MRNA TRansport	805269	806021	-1	Exosome non-catalytic core component	Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hMtr3p (EXOSC6)
S000003391	YGR159C	NSR1	""	806412	807656	-1	Nucleolin, nucleolar protein that binds nuclear localization sequences	Nucleolin, nucleolar protein that binds nuclear localization sequences; required for pre-rRNA processing and ribosome biogenesis; binds to single stranded telomeric DNA and mRNA; binds to G-quadruplex DNA; methylated by Hmt1p; interaction with Top1p and nucleolar localization are negatively regulated by polyphosphorylation
S000003392	YGR160W	""	""	807073	807684	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000003393	YGR161C	RTS3	""	808629	809420	-1	Putative component of the protein phosphatase type 2A complex	Putative component of the protein phosphatase type 2A complex
S000003394	YGR162W	TIF4631	Translation Initiation Factor	824059	826917	1	Translation initiation factor eIF4G and scaffold protein	Translation initiation factor eIF4G and scaffold protein; subunit of the mRNA cap-binding protein complex (eIF4F) with eIF4E (Cdc33p) and eIF4A (Tif1p/Tif2p); stimulates the ATPase and helicase activities of eIF4A synergistically with eIF4G; required with eIF4E for Ded1p-mediated stimulation of mRNA recruitment during 48S preinitiation complex (PIC) assembly; association with Pab1p mediates poly(A) tail-stimulated translation; role in assembly of PET region in biogenesis of 60S ribosomal subunit
S000003395	YGR163W	GTR2	GTp binding protein Resemblance	827552	828577	1	Subunit of a TORC1-stimulating GTPase and the EGO/GSE complex	Subunit of a TORC1-stimulating GTPase and the EGO/GSE complex; subunit of Gtr1-Gtr2, a GTPase that activates TORC1 in response to amino acid stimulation; negatively regulates the GTPase cycle of Gtr1p, a Ran/TC4 homolog; subunit of EGO, a vacuolar membrane complex that regulates exit from rapamycin-induced growth arrest and esorting of Gap1p; activates transcription when chromatin bound; role in the oxidative stress response; activated by the Lst4p-Lst7p GAP complex; homolog of human RagC
S000003396	YGR164W	""	""	828625	828960	1	Putative protein of unknown function	Putative protein of unknown function; conserved across S. cerevisiae strains
S000003397	YGR165W	MRPS35	Mitochondrial Ribosomal Protein, Small subunit	829116	830153	1	Mitochondrial ribosomal protein of the small subunit	Mitochondrial ribosomal protein of the small subunit; null mutant does not grow on glycerol, is sensitive to 2,4-dichlorophenol, and accumulates large lipid droplets
S000003398	YGR166W	TRS65	TRapp Subunit	830515	832197	1	Component of transport protein particle (TRAPP) complex II	Component of transport protein particle (TRAPP) complex II; TRAPPII is a multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating intra-Golgi and endosome-Golgi traffic; role in cell wall beta-glucan biosynthesis and the stress response
S000003399	YGR167W	CLC1	Clathrin Light Chain	832456	833157	1	Clathrin light chain	Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; regulates endocytic progression; thought to regulate clathrin function; the clathrin triskelion is a trimeric molecule composed of three heavy chains that radiate from a vertex and three light chains which bind noncovalently near the vertex of the triskelion
S000003400	YGR168C	PEX35	PEroXin	833352	834482	-1	Regulator of peroxisome abundance	Regulator of peroxisome abundance; peroxisomal membrane protein, remote homolog to several curvature-generating human proteins; functionally interacts with vesicle-budding-inducing ADP-ribosylation factor Arf1p
S000003401	YGR169C	PUS6	PseudoUridine Synthase	834689	835903	-1	tRNA:pseudouridine synthase	tRNA:pseudouridine synthase; catalyzes the conversion of uridine to pseudouridine at position 31 in cytoplasmic and mitochondrial tRNAs; mutation of Asp168 to Ala abolishes enzyme activity; not essential for viability
S000003402	YGR170W	PSD2	PhosphatidylSerine Decarboxylase	837142	840558	1	Phosphatidylserine decarboxylase of the Golgi and vacuolar membranes	Phosphatidylserine decarboxylase of the Golgi and vacuolar membranes; converts phosphatidylserine to phosphatidylethanolamine; controls vacuolar membrane phospholipid content by regulating phospholipids in compartments that will eventually give rise to the vacuole; loss of Psd2p causes a specific reduction in vacuolar membrane PE levels while total PE levels are not significantly affected
S000003403	YGR171C	MSM1	Mitochondrial aminoacyl-tRNA Synthetase, Methionine	840824	842551	-1	Mitochondrial methionyl-tRNA synthetase (MetRS)	Mitochondrial methionyl-tRNA synthetase (MetRS); functions as a monomer in mitochondrial protein synthesis; functions similarly to cytoplasmic MetRS although the cytoplasmic form contains a zinc-binding domain not found in Msm1p
S000003405	YGR173W	RBG2	RiBosome interacting Gtpase	843854	844960	1	Protein with a role in translation	Protein with a role in translation; forms a complex with Gir2p; has similarity to mammalian developmentally regulated GTP-binding protein
S000003406	YGR174C	CBP4	Cytochrome B mRNA Processing	845893	846405	-1	Mitochondrial protein required for assembly of cytochrome bc1 complex	Mitochondrial protein required for assembly of cytochrome bc1 complex; interacts with the Cbp3p-Cbp6p complex and newly synthesized cytochrome b (Cobp) to promote assembly of Cobp into the cytochrome bc1 complex
S000003407	YGR175C	ERG1	ERGosterol biosynthesis	846933	848423	-1	Squalene epoxidase	Squalene epoxidase; catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine; directed to the ER via the guided entry of tail-anchored proteins (GET) pathway; human SQLE functionally complements the lethality of the erg1 null mutation
S000003408	YGR176W	""	""	848720	849067	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000003409	YGR177C	ATF2	AcetylTransFerase	848829	850436	-1	Alcohol acetyltransferase	Alcohol acetyltransferase; may play a role in steroid detoxification; forms volatile esters during fermentation, which is important for brewing and winemaking
S000003410	YGR178C	PBP1	Pab1p-Binding Protein	851047	853215	-1	Component of glucose deprivation induced stress granules	Component of glucose deprivation induced stress granules; involved in P-body-dependent granule assembly; regulates TORC1 signaling and autophagy; interacts with Pab1p to regulate mRNA polyadenylation; interacts with Mkt1p to regulate HO translation; regulates expression of genes involved in gluconeogenesis and mitochondrial function through multiple mechanisms
S000003412	YGR180C	RNR4	RiboNucleotide Reductase	855264	856301	-1	Ribonucleotide-diphosphate reductase (RNR) small subunit	Ribonucleotide-diphosphate reductase (RNR) small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; relocalizes from nucleus to cytoplasm upon DNA replication stress; RNR4 has a paralog, RNR2, that arose from the whole genome duplication
S000003413	YGR181W	TIM13	Translocase of the Inner Mitochondrial membrane	858287	858604	1	Mitochondrial intermembrane space protein	Mitochondrial intermembrane space protein; forms a complex with Tim8p that delivers a subset of hydrophobic proteins to the TIM22 complex for insertion into the inner membrane
S000003414	YGR182C	""	""	858551	858904	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TIM13/YGR181W
S000003415	YGR183C	QCR9	ubiQuinol-cytochrome C oxidoReductase	859063	859476	-1	Subunit 9 of ubiquinol cytochrome-c reductase (Complex III)	Subunit 9 of ubiquinol cytochrome-c reductase (Complex III); Complex III is a component of the mitochondrial inner membrane electron transport chain; required for electron transfer at the ubiquinol oxidase site of the complex
S000003416	YGR184C	UBR1	""	859901	865753	-1	E3 ubiquitin ligase (N-recognin)	E3 Ub-ligase (N-recognin); recognizes and ubiquitinates N-end rule pathway substrates in complex with Rad6p; Roq1p-bound form accelerates degradation of misfolded and native ER membrane and cytosolic proteins as part of the stress-induced homeostatically regulated protein degradation (SHRED) pathway; role in ER-associated protein degradation; modifies Cup9p to regulate peptide transport; mutation in human UBR1 causes Johansson-Blizzard Syndrome (JBS)
S000003417	YGR185C	TYS1	TYrosyl-tRNA Synthetase	866336	867520	-1	Cytoplasmic tyrosyl-tRNA synthetase	Cytoplasmic tyrosyl-tRNA synthetase; required for cytoplasmic protein synthesis; interacts with positions 34 and 35 of the tRNATyr anticodon; mutations in human ortholog YARS are associated with Charcot-Marie-Tooth (CMT) neuropathies; human ortholog YARS functionally complements the heat sensitivity of a ts allele; protein abundance increases in response to DNA replication stress
S000003418	YGR186W	TFG1	Transcription Factor G	867774	869981	1	TFIIF (Transcription Factor II) largest subunit	TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74
S000003419	YGR187C	HGH1	HmG1/2 Homolog	870232	871416	-1	Chaperone protein for translation factor eEF2	Chaperone protein for translation factor eEF2; involved in biogenesis of eukaryotic Elongation Factor 2 (Eft1p, Eft2p); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; similar to mammalian BRP16 (Brain protein 16); relative distribution to the nucleus increases upon DNA replication stress
S000003420	YGR188C	BUB1	Budding Uninhibited by Benzimidazole	872044	875109	-1	Protein kinase involved in the cell cycle checkpoint into anaphase	Protein kinase involved in the cell cycle checkpoint into anaphase; in complex with Mad1p and Bub3p, prevents progression into anaphase in presence of spindle damage; Cdc28p-mediated phosphorylation at Bub1p-T566 is important for degradation in anaphase and adaptation of checkpoint to prolonged mitotic arrest; associates with centromere DNA via Skp1p; involved in Sgo1p relocalization in response to sister kinetochore tension; paralog MAD3 arose from whole genome duplication
S000003421	YGR189C	CRH1	Congo Red Hypersensitive	876669	878192	-1	Chitin transglycosylase	Chitin transglycosylase; functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar and functionally redundant to Utr2; localizes to sites of polarized growth; expression induced by cell wall stress
S000003422	YGR190C	""	""	880296	880661	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene HIP1/YGR191W
S000003423	YGR191W	HIP1	HIstidine Permease	880420	882231	1	High-affinity histidine permease	High-affinity histidine permease; also involved in the transport of manganese ions
S000003424	YGR192C	TDH3	Triose-phosphate DeHydrogenase	882812	883810	-1	Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), isozyme 3	Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), isozyme 3; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; GAPDH-derived antimicrobial peptides secreted by S. cerevisiae are active against a wide variety of wine-related yeasts and bacteria; binds AU-rich RNA
S000003425	YGR193C	PDX1	Pyruvate Dehydrogenase complex protein X	884509	885741	-1	E3-binding protein of the mitochondrial pyruvate dehydrogenase complex	E3-binding protein of the mitochondrial pyruvate dehydrogenase complex; plays a structural role in the complex by binding and positioning dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide acetyltransferase (E2) core
S000003426	YGR194C	XKS1	XyluloKinaSe	886073	887875	-1	Xylulokinase	Xylulokinase; converts D-xylulose and ATP to xylulose 5-phosphate and ADP; rate limiting step in fermentation of xylulose; required for xylose fermentation by recombinant S. cerevisiae strains
S000003428	YGR196C	FYV8	Function required for Yeast Viability	889733	892186	-1	Protein of unknown function	Protein of unknown function; required for survival upon exposure to K1 killer toxin
S000003429	YGR197C	SNG1	""	892497	894140	-1	Protein involved in resistance to nitrosoguanidine and 6-azauracil	Protein involved in resistance to nitrosoguanidine and 6-azauracil; expression is regulated by transcription factors involved in multidrug resistance; SNG1 has a paralog, YJR015W, that arose from the whole genome duplication
S000003430	YGR198W	YPP1	Yeast Protective Protein	894693	897146	1	Cargo-transport protein involved in endocytosis	Cargo-transport protein involved in endocytosis; interacts with phosphatidylinositol-4-kinase Stt4p; is required, along with Efr3p, for the assembly and recruitment of multiple copies of the kinase into phosphoinositide kinase (PIK) patches at the plasma membrane; positively regulates Stt4p; GFP-fusion protein localizes to the cytoplasm; YGR198W is an essential gene
S000003431	YGR199W	PMT6	Protein O-MannosylTransferase	897502	899781	1	Protein O-mannosyltransferase	Protein O-mannosyltransferase; transfers mannose from dolichyl phosphate-D-mannose to protein serine/threonine residues of secretory proteins; reaction is essential for cell wall rigidity; member of a family of mannosyltransferases
S000003432	YGR200C	ELP2	ELongator Protein	899904	902270	-1	Subunit of Elongator complex	Subunit of Elongator complex; binds to microtubules via conserved alkaline residues; has two seven-bladed WD40 &#946; propellers; Elongator complex is required for modification of wobble nucleosides in tRNA; target of Kluyveromyces lactis zymocin
S000003433	YGR201C	""	""	902520	903197	-1	Putative protein of unknown function	Putative protein of unknown function
S000003434	YGR202C	PCT1	Phosphocholine CytidylylTransferase	903474	904748	-1	Cholinephosphate cytidylyltransferase	Cholinephosphate cytidylyltransferase; a rate-determining enzyme of the CDP-choline pathway for phosphatidylcholine synthesis, inhibited by Sec14p, activated upon lipid-binding; controls phosphatidylcholine homeostasis; contains an element within the regulatory domain involved in both silencing and activation of enzymatic activity
S000003435	YGR203W	YCH1	Yeast Cdc25 Homolog	905237	905683	1	Phosphatase with sequence similarity to Mih1p	Phosphatase with sequence similarity to Mih1p; member of the Cdc25p subfamily of tyrosine phosphatases and Arr2p, an arsenate reductase, based on the presence of a rhodanese-homology domain; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
S000003436	YGR204W	ADE3	ADEnine requiring	905934	908774	1	Cytoplasmic trifunctional enzyme	Cytoplasmic trifunctional enzyme; catalyzes sequential reactions 10-formyl-THF synthetase (EC 6.3.4.3), 5,10-methenyl-THF cyclohydrolase (EC 3.5.4.9), and 5,10-methylene-THF dehydrogenase (EC 1.5.1.5), to supply forms of activated one-carbon units required for biosynthesis of purines, histidine, methionine, pantothenic acid, the formyl group of mitochondrial fMet-tRNAfMet, and the methyl group of thymidylate; null mutation causes auxotrophy for adenine and histidine in rich medium
S000003437	YGR205W	TDA10	Topoisomerase I Damage Affected	909213	910085	1	ATP-binding protein of unknown function	ATP-binding protein of unknown function; crystal structure resembles that of E.coli pantothenate kinase and other small kinases; null mutant is sensitive to expression of the top1-T722A allele
S000003438	YGR206W	MVB12	MultiVesicular Body sorting factor of 12 kilodaltons	910432	910737	1	ESCRT-I subunit required to stabilize ESCRT-I core complex oligomers	ESCRT-I subunit required to stabilize ESCRT-I core complex oligomers; the ESCRT-I core complex (Stp22p, Vps28p, Srn2p) is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin
S000003439	YGR207C	CIR1	Changed Intracellular Redox state	910843	911628	-1	Mitochondrial protein that interacts with frataxin (Yfh1p)	Mitochondrial protein that interacts with frataxin (Yfh1p); putative ortholog of mammalian electron transfer flavoprotein complex subunit ETF-beta; may have a role in oxidative stress response
S000003440	YGR208W	SER2	SERine requiring	911883	912812	1	Phosphoserine phosphatase of the phosphoglycerate pathway	Phosphoserine phosphatase of the phosphoglycerate pathway; involved in serine and glycine biosynthesis, expression is regulated by the available nitrogen source
S000003441	YGR209C	TRX2	ThioRedoXin	912913	913227	-1	Cytoplasmic thioredoxin isoenzyme	Cytoplasmic thioredoxin isoenzyme; part of the thioredoxin system that protects cells from oxidative and reductive stress; subunit of the LMA1 complex, with Pbi2p, that promotes vacuole inheritance; cofactor for Tsa1p; required for ER-Golgi transport; facilitates mitochondrial import of small Tims (Tim9p, Tim10p, Tim13p) by maintaining them in reduced form along with Trx1p; component of a signal transduction redox relay in a light sensing pathway; abundance increases under DNA replication stress
S000003442	YGR210C	""	""	913503	914738	-1	Putative protein of unknown function	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
S000003443	YGR211W	ZPR1	Zinc finger PRotein	915241	916701	1	Chaperone with two zinc fingers essential for eEF1A biogenesis	Chaperone with two zinc fingers essential for eEF1A biogenesis; nuclear in growing cells, cytoplasmic in starved cells; shuttled via process mediated by Cpr1p; binds translation elongation factor eEF-1 (Tef1p); relative distribution to nucleus increases upon DNA replication stress; human ZPR1 gene can complement yeast by allowing growth during down-regulation of yeast zpr1
S000003444	YGR212W	SLI1	Sphingosine-Like Immunosuppressant resistant gene	917040	918446	1	N-acetyltransferase	N-acetyltransferase; confers resistance to the sphingolipid biosynthesis inhibitor myriocin (ISP-1) by converting it into N-acetyl-myriocin, co-operates with Ypk1p in mediating resistance to myriocin
S000003445	YGR213C	RTA1	Resistance To 7-Aminocholesterol	918512	919465	-1	Protein involved in 7-aminocholesterol resistance	Protein involved in 7-aminocholesterol resistance; has seven potential membrane-spanning regions; expression is induced under both low-heme and low-oxygen conditions; member of the fungal lipid-translocating exporter (LTE) family of protein; RTA1 has a paralog, YLR046C, that arose from the whole genome duplication
S000003446	YGR214W	RPS0A	Ribosomal Protein of the Small subunit	920575	921788	1	Ribosomal 40S subunit protein S0A	Ribosomal 40S subunit protein S0A; required for maturation of 18S rRNA along with Rps0Bp; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2; RPS0A has a paralog, RPS0B, that arose from the whole genome duplication;
S000003447	YGR215W	RSM27	Ribosomal Small subunit of Mitochondria	922175	922507	1	Mitochondrial ribosomal protein of the small subunit	Mitochondrial ribosomal protein of the small subunit
S000003449	YGR217W	CCH1	Calcium Channel Homolog	924696	930815	1	Voltage-gated high-affinity calcium channel	Voltage-gated high-affinity calcium channel; involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and partially co-localizes with Mid1p; however, evidence suggests CCH1 is not required for Mid1p function
S000003451	YGR219W	""	""	936035	936376	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRPL9/YGR220C
S000003452	YGR220C	MRPL9	Mitochondrial Ribosomal Protein, Large subunit	936074	936883	-1	Mitochondrial ribosomal protein of the large subunit	Mitochondrial ribosomal protein of the large subunit
S000003453	YGR221C	TOS2	Target Of Sbf	937124	938992	-1	Protein involved in localization of Cdc24p to the site of bud growth	Protein involved in localization of Cdc24p to the site of bud growth; may act as a membrane anchor; localizes to the bud neck and bud tip; potentially phosphorylated by Cdc28p; TOS2 has a paralog, SKG6, that arose from the whole genome duplication
S000003454	YGR222W	PET54	PETite colonies	939923	940804	1	Mitochondrial inner membrane protein	Mitochondrial inner membrane protein; binds to the 5' UTR of the COX3 mRNA to activate its translation together with Pet122p and Pet494p; also binds to the COX1 Group I intron AI5 beta to facilitate exon ligation during splicing; also stimulates efficient synthesis of Cox1p
S000003455	YGR223C	HSV2	Homologous with SVP1	940869	942215	-1	Phosphatidylinositol 3,5-bisphosphate-binding protein	Phosphoinositide (PI) binding protein; involved in micronucleophagy; belongs to the PROPPIN family of proteins defined by a seven-bladed beta-propeller fold and FRRG motif required for PI binding; displays punctate cytoplasmic localization
S000003456	YGR224W	AZR1	Acetic Acid and AZoles Resistance	942806	944647	1	Plasma membrane transporter of the major facilitator superfamily	Plasma membrane transporter of the major facilitator superfamily; involved in resistance to azole drugs such as ketoconazole and fluconazole
S000003457	YGR225W	AMA1	Activator of Meiotic APC/C	945145	947019	1	Activator of meiotic anaphase promoting complex (APC/C)	Activator of meiotic anaphase promoting complex (APC/C); Cdc20p family member; required for initiation of spore wall assembly; required for Clb1p degradation during meiosis; prevents premature assembly of the meiosis I spindle, required for DSB induced prophase I arrest
S000003458	YGR226C	""	""	946792	947001	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; overlaps significantly with a verified ORF, AMA1/YGR225W
S000003459	YGR227W	DIE2	Derepression of ITR1 Expression	947420	948997	1	Dolichyl-phosphoglucose-dependent alpha-1,2-glucosyltransferase	Dolichyl-phosphoglucose-dependent alpha-1,2-glucosyltransferase; located in the ER; functions in pathway that synthesizes the dolichol-linked oligosaccharide precursor for N-linked protein glycosylation; has a role in regulation of ITR1 and INO1; human homolog ALG10B can complement yeast die2 null mutant
S000003460	YGR228W	""	""	949365	949709	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SMI1/YGR229C
S000003461	YGR229C	SMI1	Suppressor of MAR Inhibitor	949382	950899	-1	Protein involved in the regulation of cell wall synthesis	Protein involved in the regulation of cell wall synthesis; proposed to be involved in coordinating cell cycle progression with cell wall integrity
S000003462	YGR230W	BNS1	Bypasses Need for Spo12p	951894	952307	1	Protein of unknown function	Protein of unknown function; FEAR network component that partially overlaps the function of SPO12 in promoting Cdc14p release from the nucleolus during early mitotic anaphase and mitotic exit; overexpression partially suppresses the meiotic defect of spo12; BNS1 has a paralog, SPO12, that arose from the whole genome duplication
S000003463	YGR231C	PHB2	ProHiBitin	952548	953480	-1	Subunit of the prohibitin complex (Phb1p-Phb2p)	Subunit of the prohibitin complex (Phb1p-Phb2p); prohibitin is a 1.2 MDa ring-shaped inner mitochondrial membrane chaperone that stabilizes newly synthesized proteins; determinant of replicative life span; involved in mitochondrial segregation; prohibitin deficiency induces a mitochondrial unfolded protein response (mtUPR)
S000003464	YGR232W	NAS6	Non-ATPase Subunit	953960	954646	1	Evolutionarily conserved 19S regulatory particle assembly-chaperone	Evolutionarily conserved 19S regulatory particle assembly-chaperone; proteasome-interacting protein involved in the assembly of the base subcomplex of the 19S proteasomal regulatory particle (RP); ortholog of human oncoprotein gankyrin, also known as p28, which interacts with the Rb tumor suppressor and CDK4/6
S000003465	YGR233C	PHO81	PHOsphate metabolism	954674	958210	-1	Cyclin-dependent kinase (CDK) inhibitor	Cyclin-dependent kinase (CDK) inhibitor; regulates Pho80p-Pho85p and Pcl7p-Pho85p cyclin-CDK complexes in response to phosphate levels; inhibitory activity for Pho80p-Pho85p requires myo-D-inositol heptakisphosphate (IP7) generated by Vip1p; relative distribution to the nucleus increases upon DNA replication stress
S000003466	YGR234W	YHB1	Yeast flavoHemogloBin	959904	961103	1	Nitric oxide oxidoreductase	Nitric oxide oxidoreductase; flavohemoglobin that plays role in oxidative and nitrosative stress responses; protects against nitration of cellular targets and against cell growth inhibition under aerobic or anaerobic conditions; yeast flavohemoglobin Yhb1p and human homolog neuroglobin NGB protect cells against alpha-synuclein cytotoxicity and aggregate formation; protein increases in abundance, relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
S000003467	YGR235C	MIC26	MItochondrial contact site and Cristae organizing system	961360	962061	-1	Component of the MICOS complex	Component of the MICOS complex; MICOS (formerly MINOS or MitOS) is a mitochondrial inner membrane complex that extends into the intermembrane space and has a role in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane; Mic26p is a non-essential component of the complex; orthologous to human APOO and APOOL
S000003468	YGR236C	SPG1	Stationary Phase Gene	962530	962817	-1	Protein required for high temperature survival during stationary phase	Protein required for high temperature survival during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
S000003469	YGR237C	""	""	963298	965655	-1	Putative protein of unknown function	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
S000003470	YGR238C	KEL2	KELch repeat	966039	968687	-1	Protein that negatively regulates mitotic exit	Protein that negatively regulates mitotic exit; forms a complex with Kel1p and Bud14p that regulates Bnr1p (formin) to affect actin cable assembly, cytokinesis, and polarized growth; functions in a complex with Kel1p, interacts with Tem1p and Lte1p; localizes to regions of polarized growth; potential Cdc28p substrate
S000003471	YGR239C	PEX21	PEroXin	969187	970053	-1	Peroxin required for peroxisomal matrix protein targeting	Peroxin required for peroxisomal matrix protein targeting; acts on proteins containing the PTS2 targeting sequence; interacts with Pex7p; constitutively expressed; partially redundant with Pex18p; required for import of the Gpd1p-Pnc1p heterodimer in which only Gpd1p has a peroxisomal targeting signal; relative distribution to cytoplasmic foci increases upon DNA replication stress
S000003472	YGR240C	PFK1	PhosphoFructoKinase	970771	973734	-1	Alpha subunit of heterooctameric phosphofructokinase	Alpha subunit of heterooctameric phosphofructokinase; involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes
S000003473	YGR241C	YAP1802	Yeast Assembly Polypeptide	974875	976581	-1	Protein of the AP180 family, involved in clathrin cage assembly	Protein of the AP180 family, involved in clathrin cage assembly; binds Pan1p and clathrin; YAP1802 has a paralog, YAP1801, that arose from the whole genome duplication
S000003474	YGR242W	""	""	976415	976723	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YAP1802/YGR241C
S000003475	YGR243W	MPC3	Mitochondrial Pyruvate Carrier	977336	977776	1	Highly conserved subunit of the mitochondrial pyruvate carrier (MPC)	Highly conserved subunit of the mitochondrial pyruvate carrier (MPC); expressed during growth on nonfermentable carbon sources, and heterodimerizes with Mpc1p to form the respiratory isoform of MPC; MPC localizes to the mitochondrial inner membrane and mediates pyruvate uptake; MPC3 paralog, MPC2, heterodimerizes with Mpc1p to form the fermentative MPC isoform; protein abundance increases in response to DNA replication stress
S000003476	YGR244C	LSC2	Ligase of Succinyl-CoA	978036	979319	-1	Beta subunit of succinyl-CoA ligase	Beta subunit of succinyl-CoA ligase; succinyl-CoA ligase is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate
S000003477	YGR245C	SDA1	Severe Depolymerization of Actin	979765	982068	-1	Protein required for actin organization and passage through Start	Protein required for actin organization and passage through Start; highly conserved nuclear protein; required for actin cytoskeleton organization; plays a critical role in G1 events; binds Nap1p; involved in 60S ribosome biogenesis
S000003478	YGR246C	BRF1	B-Related Factor	982482	984272	-1	TFIIIB B-related factor	TFIIIB B-related factor; one of three subunits of RNA polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB; mutations in human homolog are associated with autosomal recessive cerebellar-facial-dental syndrome
S000003479	YGR247W	CPD1	Cyclic nucleotide PhosphoDiesterase	984966	985685	1	Cyclic nucleotide phosphodiesterase	Cyclic nucleotide phosphodiesterase; hydrolyzes ADP-ribose 1'', 2''-cyclic phosphate to ADP-ribose 1''-phosphate; may have a role in tRNA splicing; no detectable phenotype is conferred by null mutation or by overexpression; protein abundance increases in response to DNA replication stress
S000003480	YGR248W	SOL4	Suppressor Of Los1-1	985972	986739	1	6-phosphogluconolactonase	6-phosphogluconolactonase; protein abundance increases in response to DNA replication stress; SOL4 has a paralog, SOL3, that arose from the whole genome duplication
S000003481	YGR249W	MGA1	""	988049	989419	1	Protein similar to heat shock transcription factor	Protein similar to heat shock transcription factor; multicopy suppressor of pseudohyphal growth defects of ammonium permease mutants
S000003482	YGR250C	RIE1	Restoration of Impaired growths of ERMES-lacking cells	991176	993521	-1	RNA binding protein and negative regulator of START	RNA binding protein and negative regulator of START; binds mRNA of the G1 cyclin CLN3 to stress granules for translational repression under conditions of stress; recruits the cyclin-dependent protein kinase Cdc28p to stress granules when stressful conditions are relieved, resulting in the dissolution of the granules, and resumption of translation; localizes to stress granules induced by glucose deprivation; protein abundance increases in response to DNA replication stress
S000003483	YGR251W	NOP19	NucleOlar Protein	995639	996229	1	Ribosome biogenesis factor	Ribosome biogenesis factor; nucleolar protein associated with pre-rRNA components of the 90S preribosome, required for cleavage of pre-rRNA at A0, A1 and A2 sites; interacts with RNA helicase Dhr2p and RNA helicase-like protein Utp25p; required for incorporation of Utp25p into preribosomes
S000003484	YGR252W	GCN5	General Control Nonderepressible	996869	998188	1	Catalytic subunit of ADA and SAGA histone acetyltransferase complexes	Catalytic subunit of ADA and SAGA histone acetyltransferase complexes; modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; relocalizes to the cytosol in response to hypoxia; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation
S000003486	YGR254W	ENO1	ENOlase	1000927	1002240	1	Enolase I, a phosphopyruvate hydratase	Enolase I, a phosphopyruvate hydratase; catalyzes conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression repressed in response to glucose; protein abundance increases in response to DNA replication stress; N-terminally propionylated in vivo; ENO1 has a paralog, ENO2, that arose from the whole genome duplication
S000003487	YGR255C	COQ6	COenzyme Q	1002523	1003962	-1	Flavin-dependent monooxygenase involved in ubiquinone biosynthesis	Flavin-dependent monooxygenase involved in ubiquinone biosynthesis; responsible for hydroxylation at position C5 and deamination at C4 during ubiquinone (Coenzyme Q) biosynthesis; localizes to matrix face of mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes; human homolog COQ6 can complement yeast null mutant and is implicated in steroid-resistant nephrotic syndrome (SRNS)
S000003488	YGR256W	GND2	6-phosphoGlucoNateDehydrogenase	1004624	1006102	1	6-phosphogluconate dehydrogenase (decarboxylating)	6-phosphogluconate dehydrogenase (decarboxylating); catalyzes an NADPH regenerating reaction in the pentose phosphate pathway; required for growth on D-glucono-delta-lactone; GND2 has a paralog, GND1, that arose from the whole genome duplication
S000003489	YGR257C	MTM1	Manganese Trafficking factor for Mitochondrial SOD2	1006205	1007305	-1	Mitochondrial protein of the mitochondrial carrier family	Mitochondrial protein of the mitochondrial carrier family; high affinity pyridoxal 5'-phosphate (PLP) transporter, important for delivery of the PLP cofactor to mitochondrial enzymes; involved in mitochondrial iron homeostasis
S000003490	YGR258C	RAD2	RADiation sensitivity	1007671	1010766	-1	Single-stranded DNA endonuclease	Single-stranded DNA endonuclease; cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein
S000003491	YGR259C	""	""	1012481	1012921	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF TNA1/YGR260W
S000003492	YGR260W	TNA1	Transporter of Nicotinic Acid	1012485	1014089	1	High affinity nicotinic acid plasma membrane permease	High affinity nicotinic acid plasma membrane permease; responsible for uptake of low levels of nicotinic acid; expression of the gene increases in the absence of extracellular nicotinic acid or para-aminobenzoate (PABA)
S000003493	YGR261C	APL6	clathrin Adaptor Protein complex Large chain	1014321	1016750	-1	Beta3-like subunit of the yeast AP-3 complex	Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools
S000003494	YGR262C	BUD32	BUD site selection	1016974	1017759	-1	Component of KEOPS complex with Kae1p, Cgi121p, Pcc1p, and Gon7p	Component of KEOPS complex with Kae1p, Cgi121p, Pcc1p, and Gon7p; provides the ATPase and GTPase activities of the complex; EKC/KEOPS complex is required for t6A tRNA modification and telomeric TG1-3 recombination; may have role in transcription; mutation is functionally complemented by human ortholog TP53RK
S000003495	YGR263C	SAY1	Steryl Acetyl hYdrolase	1017967	1019241	-1	Sterol deacetylase	Sterol deacetylase; component of the sterol acetylation/deacetylation cycle along with Atf2p; active both in the endoplasmic reticulum (ER) and in lipid droplets; integral membrane protein with active site in the ER lumen; green fluorescent protein (GFP)-fusion protein localizes to the ER
S000003496	YGR264C	MES1	MEthionyl-tRNA Synthetase	1019598	1021853	-1	Methionyl-tRNA synthetase	Methionyl-tRNA synthetase; forms a complex with Gus1p, a glutamyl-tRNA synthetase, and Arc1p, which increases the catalytic efficiency of both synthetases; involved in the nuclear export of tRNAs; mutations in human ortholog MARS is associated with pediatric pulmonary alveolar proteinosis, interstitial lung and liver disease, and Charcot-Marie-Tooth disease; human MARS gene can complement the yeast null mutant
S000003497	YGR265W	""	""	1021648	1022058	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MES1/YGR264C, which encodes methionyl-tRNA synthetase
S000003498	YGR266W	""	""	1022656	1024761	1	Protein of unknown function	Protein of unknown function; predicted to contain a single transmembrane domain; mutant has increased aneuploidy tolerance; localized to both the mitochondrial outer membrane and the plasma membrane; protein abundance increases in response to DNA replication stress
S000003499	YGR267C	FOL2	FOLic acid synthesis	1025004	1025735	-1	GTP-cyclohydrolase I, catalyzes first step in folic acid biosynthesis	GTP-cyclohydrolase I, catalyzes first step in folic acid biosynthesis; human homolog GCH1 is implicated in dopa-responsive dystonia (DRD), and can complement yeast null mutant
S000003500	YGR268C	HUA1	""	1026057	1026653	-1	Cytoplasmic protein containing a zinc finger domain	Cytoplasmic protein containing a zinc finger domain; sequence similarity to that of Type I J-proteins; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly
S000003501	YGR269W	""	""	1026636	1026962	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF HUA1/YGR268C
S000003502	YGR270W	YTA7	Yeast Tat-binding Analog	1027370	1031509	1	Chromatin segregase involved in DNA replication	Chromatin segregase involved in DNA replication; ATPase activity, stimulated by S phase CDK-dependent phosphorylation, is coupled to nucleosome disassembly, promoting DNA replication; regulates cell-cycle-dependent histone gene expression; contains AAA+-ATPase domains and a non-canonical bromodomain; hexameric; relocalizes to the cytosol in response to hypoxia; human ortholog ATAD2 is a coregulator of oncogenic transcription factors and an oncogene overexpressed in cancers with poor prognosis
S000003503	YGR271W	SLH1	SKI2-Like Helicase	1031791	1037694	1	Subunit of ribosome-associated quality control trigger complex (RQT)	Subunit of ribosome-associated quality control trigger complex (RQT); putative RNA helicase related to Ski2p; involved in translation inhibition of non-poly(A) mRNAs; required for repressing propagation of dsRNA viruses; human homolog is ASCC3
S000003504	YGR272C	""	""	1038048	1038506	-1	Merged open reading frame	Merged open reading frame; does not encode a discrete protein;YGR272C was originally annotated as an independent ORF, but as a result of a sequence change, it was merged with an adjacent ORF into a single reading frame, designated YGR271C-A
S000003505	YGR273C	""	""	1038715	1039239	-1	Putative protein of unknown function	Putative protein of unknown function; expression downregulated by treatment with 8-methoxypsoralen plus UVA irradiation; not an essential gene; YGR273C has a paralog, YMR295C, that arose from the whole genome duplication
S000003507	YGR275W	RTT102	Regulator of Ty1 Transposition	1043276	1043749	1	Component of both the SWI/SNF and RSC chromatin remodeling complexes	Component of both the SWI/SNF and RSC chromatin remodeling complexes; suggested role in chromosome maintenance; possible weak regulator of Ty1 transposition; protein abundance increases in response to DNA replication stress
S000003508	YGR276C	RNH70	RNase H	1043819	1045480	-1	3'-5' exoribonuclease	3'-5' exoribonuclease; required for maturation of 3' ends of 5S rRNA and tRNA-Arg3 from dicistronic transcripts
S000003510	YGR278W	CWC22	Complexed With Cef1p	1046731	1048464	1	Spliceosome-associated protein that is required for pre-mRNA splicing	Spliceosome-associated protein that is required for pre-mRNA splicing; necessary for Prp2p function at the first catalytic step of splicing; has similarity to S. pombe Cwf22p; CWC22 is an essential protein
S000003511	YGR279C	SCW4	Soluble Cell Wall protein	1048798	1049958	-1	Cell wall protein with similarity to glucanases	Cell wall protein with similarity to glucanases; targeted to vacuole via AP-3 pathway; scw4 scw10 double mutants exhibit defects in mating; SCW4 has a paralog, SCW10, that arose from the whole genome duplication
S000003513	YGR281W	YOR1	Yeast Oligomycin Resistance	1052824	1057257	1	Plasma membrane ATP-binding cassette (ABC) transporter	Plasma membrane ATP-binding cassette (ABC) transporter; multidrug transporter mediates export of many different organic anions including oligomycin; homolog of human cystic fibrosis transmembrane receptor (CFTR)
S000003514	YGR282C	BGL2	Beta-GLucanase	1057783	1058724	-1	Endo-beta-1,3-glucanase	Endo-beta-1,3-glucanase; major protein of the cell wall, involved in cell wall maintenance; involved in incorporation of newly synthesized mannoprotein molecules into the cell wall
S000003515	YGR283C	UPA1	Urb2 particle associated methyl-transferase 	1059015	1060040	-1	Putative methyltransferase	Putative methyltransferase; may interact with ribosomes, based on co-purification experiments; predicted to be involved in ribosome biogenesis; null mutant is resistant to fluconazole; GFP-fusion protein localizes to the nucleolus; YGR283C has a paralog, YMR310C, that arose from the whole genome duplication
S000003516	YGR284C	ERV29	ER Vesicle	1060658	1061590	-1	Protein localized to COPII-coated vesicles	Protein localized to COPII-coated vesicles; involved in vesicle formation and incorporation of specific secretory cargo; protein abundance increases in response to DNA replication stress
S000003517	YGR285C	ZUO1	ZUOtin	1061852	1063153	-1	Ribosome-associated chaperone	Ribosome-associated chaperone; zuotin functions in ribosome biogenesis and as a chaperone for nascent polypeptide chains in partnership with Ssz1p and SSb1/2; contains a DnaJ domain and functions as a J-protein partner for Ssb1p and Ssb2p; human gene DNAJC2 can partially complement yeast zuo1 null mutant
S000003518	YGR286C	BIO2	BIOtin	1063813	1064940	-1	Biotin synthase	Biotin synthase; catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant
S000003519	YGR287C	IMA1	IsoMAltase	1067222	1068991	-1	Major isomaltase (alpha-1,6-glucosidase/alpha-methylglucosidase)	Major isomaltase (alpha-1,6-glucosidase/alpha-methylglucosidase); required for isomaltose utilization; preferred specificity for isomaltose, alpha-methylglucoside, and palatinose, but also exhibits alpha-1,2 glucosidase activity on sucrose and kojibiose, and can cleave the 1,3-alpha linkage of nigerose and turanose and the alpha-1,5 linkage of leucrose in vitro; member of the IMA isomaltase family
S000003520	YGR288W	MAL13	MALtose fermentation	1070293	1071714	1	MAL-activator protein	MAL-activator protein; part of complex locus MAL1; nonfunctional in genomic reference strain S288C
S000003521	YGR289C	MAL11	MALtose fermentation	1073963	1075813	-1	High-affinity maltose transporter (alpha-glucoside transporter)	High-affinity maltose transporter (alpha-glucoside transporter); inducible; encoded in the MAL1 complex locus; broad substrate specificity that includes maltotriose; required for isomaltose utilization
S000003522	YGR290W	""	""	1075482	1075925	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; putative HLH protein; partially overlaps the verified ORF MAL11/YGR289C (a high-affinity maltose transporter)
S000003523	YGR291C	""	""	1076283	1076504	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000003524	YGR292W	MAL12	MALtose fermentation	1076599	1078353	1	Maltase (alpha-D-glucosidase)	Maltase (alpha-D-glucosidase); inducible protein involved in maltose catabolism; encoded in the MAL1 complex locus; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose
S000003527	YGR295C	COS6	COnserved Sequence	1081584	1082729	-1	Endosomal protein involved in turnover of plasma membrane proteins	Endosomal protein involved in turnover of plasma membrane proteins; member of the DUP380 subfamily of conserved, often subtelomeric COS genes; required for the multivesicular vesicle body sorting pathway that internalizes plasma membrane proteins for degradation; Cos proteins provide ubiquitin in trans for nonubiquitinated cargo proteins
S000003529	YHR001W-A	QCR10	ubiQuinol-cytochrome C oxidoReductase	107826	108122	1	Subunit of the ubiqunol-cytochrome c oxidoreductase complex	Subunit of the ubiqunol-cytochrome c oxidoreductase complex; this complex comprises part of the mitochondrial respiratory chain; members include Cobp, Rip1p, Cyt1p, Cor1p, Qcr2p, Qcr6p, Qcr7p, Qcr8p, Qcr9p, and Qcr10p and comprises part of the mitochondrial respiratory chain
S000003530	YHR005C-A	TIM10	Translocase of the Inner Membrane	115620	115901	-1	Essential protein of the mitochondrial intermembrane space	Essential protein of the mitochondrial intermembrane space; forms a complex with Tim9p (TIM10 complex) that delivers hydrophobic proteins to the TIM22 complex for insertion into the inner membrane
S000003531	YHR021W-A	ECM12	ExtraCellular Mutant	149225	149680	1	Putative protein of unknown function	Putative protein of unknown function; may contribute to cell wall biosynthesis, mutants display zymolyase hypersensitivity
S000003532	YHR049C-A	""	""	207234	207530	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000003533	YHR139C-A	""	""	380108	380419	-1	Putative protein of unknown function	Putative protein of unknown function; conserved across S. cerevisiae strains
S000003536	YIL014C-A	""	""	325212	325526	-1	Putative protein of unknown function	Putative protein of unknown function; null mutant shows improved incorporation efficiency of noncanonical amino acids in place of amber stop codon
S000003538	YJL001W	PRE3	PRoteinase yscE	435163	435926	1	Beta 1 subunit of the 20S proteasome	Beta 1 subunit of the 20S proteasome; responsible for cleavage after acidic residues in peptides
S000003539	YJL002C	OST1	OligoSaccharylTransferase	433221	434651	-1	Alpha subunit of the oligosaccharyltransferase complex of the ER lumen	Alpha subunit of the oligosaccharyltransferase complex of the ER lumen; complex catalyzes asparagine-linked glycosylation of newly synthesized proteins
S000003540	YJL003W	COX16	Cytochrome c OXidase	432644	433000	1	Mitochondrial inner membrane protein	Mitochondrial inner membrane protein; required for assembly of cytochrome c oxidase
S000003541	YJL004C	SYS1	Suppressor of Ypt Six	431589	432200	-1	Integral membrane protein of the Golgi	Integral membrane protein of the Golgi; required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation
S000003542	YJL005W	CYR1	CYclic AMP Requirement	425157	431237	1	Adenylate cyclase	Adenylate cyclase; required for cAMP production and cAMP-dependent protein kinase signaling; scaffold protein for Ras2-Ira interaction; the cAMP pathway controls a variety of cellular processes, including metabolism, cell cycle, stress response, stationary phase, and sporulation
S000003543	YJL006C	CTK2	Carboxy-Terminal domain Kinase	423138	424109	-1	Beta subunit of C-terminal domain kinase I (CTDK-I)	Beta subunit of C-terminal domain kinase I (CTDK-I); which phosphorylates both RNA pol II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and ribosomal protein Rps2p to increase translational fidelity; relocalizes to the cytosol in response to hypoxia
S000003544	YJL007C	""	""	422698	423012	-1	Putative protein of unknown function	Putative protein of unknown function; conserved among S. cerevisiae strains
S000003545	YJL008C	CCT8	Chaperonin Containing TCP-1	419957	421663	-1	Subunit of the cytosolic chaperonin Cct ring complex	Subunit of the cytosolic chaperonin Cct ring complex; related to Tcp1p, required for the assembly of actin and tubulins in vivo
S000003546	YJL009W	""	""	419855	420181	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps CCT8/YJL008C, a verified gene encoding a subunit of the cytosolic chaperonin Cct ring complex
S000003547	YJL010C	NOP9	NucleOlar Protein	417562	419562	-1	Essential subunit of U3-containing 90S preribosome	Essential subunit of U3-containing 90S preribosome; involved in production of 18S rRNA and assembly of small ribosomal subunit; also part of pre-40S ribosome and required for its export into cytoplasm; binds RNA and contains pumilio domain
S000003548	YJL011C	RPC17	RNA Polymerase C	414285	414770	-1	RNA polymerase III subunit C17	RNA polymerase III subunit C17; physically interacts with C31, C11, and TFIIIB70; may be involved in the recruitment of pol III by the preinitiation complex; protein abundance increases in response to DNA replication stress; relocalizes to the cytosol in response to hypoxia
S000003549	YJL012C	VTC4	Vacuolar Transporter Chaperone	411234	413399	-1	Vacuolar membrane polyphosphate polymerase	Vacuolar membrane polyphosphate polymerase; subunit of vacuolar transporter chaperone (VTC) complex involved in synthesis and transfer of polyP to vacuole; regulates membrane trafficking; role in non-autophagic vacuolar fusion; targeted to vacuole via AP-3 pathway; protein abundance increases in response to DNA replication stress
S000003550	YJL013C	MAD3	Mitotic Arrest-Deficient	409493	411040	-1	Subunit of spindle-assembly checkpoint complex	Subunit of spindle-assembly checkpoint complex; involved in delaying anaphase onset in cells with defects in mitotic spindle assembly; pseudosubstrate inhibitor of APC(Cdc20), the anaphase promoting complex involved in securin (Pds1p) turnover; MAD3 has a paralog, BUB1, that arose from the whole genome duplication
S000003551	YJL014W	CCT3	Chaperonin Containing TCP-1	407558	409162	1	Subunit of the cytosolic chaperonin Cct ring complex	Subunit of the cytosolic chaperonin Cct ring complex; related to Tcp1p, required for the assembly of actin and tubulins in vivo; capable of binding Q/N rich proteins and mediating their folding
S000003552	YJL015C	""	""	407142	407426	-1	Dubious open reading frame unlikely to encode a functional protein	Dubious open reading frame unlikely to encode a functional protein; expression if heat-inducible; located in promoter region of essential CCT3 gene encoding a subunit of the cytosolic chaperonin Cct ring complex, overlaps ORF YJL016W
S000003553	YJL016W	TPH3	Twin PH domain-3	405588	407273	1	Putative protein of unknown function	Putative protein of unknown function; GFP-fusion protein localizes to the cytoplasm; contains two adjacent PH-like domains; conserved in closely related Saccharomyces species
S000003554	YJL017W	""	""	405284	406261	1	Merged open reading frame	Merged open reading frame; does not encode a discrete protein; YJL017W was originally annotated as an independent ORF, but as a result of a sequence change, it was merged with an adjacent ORF into a single reading frame, designated YJL016W
S000003555	YJL018W	""	""	404326	404640	1	Merged open reading frame	Merged open reading frame; does not encode a discrete protein; YJL018W was originally annotated as an independent ORF, but as a result of a sequence change, it was merged with an adjacent ORF into a single reading frame, designated YJL019W
S000003557	YJL020C	BBC1	Bni1 synthetic lethal and Bee1 (las17) Complex member	398937	402410	-1	Protein possibly involved in assembly of actin patches	Protein possibly involved in assembly of actin patches; interacts with an actin assembly factor Las17p and with the SH3 domains of Type I myosins Myo3p and Myo5p; localized predominantly to cortical actin patches
S000003558	YJL021C	""	""	398633	399730	-1	Merged open reading frame	Merged open reading frame; does not encode a discrete protein; YJL021C was originally annotated as an independent ORF but due to a sequence change was merged with an adjacent ORF into a single reading frame, designated YJL020C/BBC1
S000003559	YJL022W	""	""	398109	398417	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PET130
S000003560	YJL023C	PET130	PETite colonies	397355	398398	-1	Protein of unknown function	Protein of unknown function; required for efficient 5' processing of mitochondrial tRNAs, for respiratory growth and mitochondrial genome maintenance; localizes to the matrix side of the inner mitochondrial membrane
S000003561	YJL024C	APS3	clathrin Associated Protein complex Small subunit	395931	396592	-1	Small subunit of the clathrin-associated adaptor complex AP-3	Small subunit of the clathrin-associated adaptor complex AP-3; involved in vacuolar protein sorting; related to the sigma subunit of the mammalian clathrin AP-3 complex; suppressor of loss of casein kinase 1 function; protein abundance increases in response to DNA replication stress
S000003562	YJL025W	RRN7	Regulation of RNA polymerase I	393967	395511	1	Component of the core factor (CF) rDNA transcription factor complex	Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p
S000003563	YJL026W	RNR2	RiboNucleotide Reductase	392404	393603	1	Ribonucleotide-diphosphate reductase (RNR), small subunit	Ribonucleotide-diphosphate reductase (RNR), small subunit; the RNR complex catalyzes the rate-limiting step in dNTP synthesis and is regulated by DNA replication and DNA damage checkpoint pathways via localization of the small subunits; RNR2 has a paralog, RNR4, that arose from the whole genome duplication
S000003564	YJL027C	""	""	391833	392249	-1	Putative protein of unknown function	Putative protein of unknown function
S000003565	YJL028W	""	""	391311	391646	1	Protein of unknown function	Protein of unknown function; may interact with ribosomes, based on co-purification experiments
S000003566	YJL029C	VPS53	Vacuolar Protein Sorting	388385	390853	-1	Component of the GARP (Golgi-associated retrograde protein) complex	Component of the GARP (Golgi-associated retrograde protein) complex; GARP is required for the recycling of proteins from endosomes to the late Golgi, and for mitosis after DNA damage induced checkpoint arrest; required for vacuolar protein sorting; members of the GARP complex are Vps51p-Vps52p-Vps53p-Vps54p; human ortholog is implicated in progressive cerebello-cerebral atrophy type 2 (PCCA2)
S000003567	YJL030W	MAD2	Mitotic Arrest-Deficient	387657	388247	1	Component of the spindle-assembly checkpoint complex	Component of the spindle-assembly checkpoint complex; delays onset of anaphase in cells with defects in mitotic spindle assembly; forms a complex with Mad1p; regulates APC/C activity during prometaphase and metaphase of meiosis I; gene dosage imbalance between MAD1 and MAD2 leads to chromosome instability
S000003568	YJL031C	BET4	Blocked Early in Transport	386368	387438	-1	Alpha subunit of Type II geranylgeranyltransferase	Alpha subunit of Type II geranylgeranyltransferase; required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p
S000003569	YJL032W	""	""	386348	386662	1	Dubious open reading frame unlikely to encode a functional protein	Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential BET4 gene encoding the alpha subunit of Type II geranylgeranyltransferase
S000003570	YJL033W	HCA4	Helicase CA	383837	386149	1	DEAD box RNA helicase	DEAD box RNA helicase; component of the SSU; interacts with Bfr2p and Enp2p; high-copy number suppression of a U14 snoRNA processing mutant suggests an involvement in 18S rRNA synthesis
S000003571	YJL034W	KAR2	KARyogamy	381327	383375	1	ATPase involved in protein import into the ER	ATPase involved in protein import into the ER; also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p
S000003572	YJL035C	TAD2	tRNA-specific Adenosine Deaminase	380249	381001	-1	Subunit of tRNA-specific adenosine-34 deaminase	Subunit of tRNA-specific adenosine-34 deaminase; forms a heterodimer with Tad3p that converts adenosine to inosine at the wobble position of several tRNAs
S000003573	YJL036W	SNX4	Sorting NeXin	378825	380096	1	Sorting nexin	Sorting nexin; involved in retrieval of late-Golgi SNAREs from post-Golgi endosomes to the trans-Golgi network and in cytoplasm to vacuole transport; contains a PX phosphoinositide-binding domain; forms complexes with Snx41p and with Atg20p
S000003574	YJL037W	IRC18	Increased Recombination Centers	376662	377336	1	Protein involved in outer spore wall assembly	Protein involved in outer spore wall assembly; possible role in assembly of the dityrosine layer; similar to adjacent ORF, LOH1; irc18 loh1 double mutant exhibits reduced dityrosine fluorescence relative to single mutants; SWAT-GFP fusion protein localizes to the ER and vacuole, while mCherry fusion localizes to the vacuole; expression induced in respiratory-deficient cells and carbon-limited chemostat culture; null mutant displays increased levels of spontaneous Rad52p foci
S000003575	YJL038C	LOH1	Loss Of Heterozygosity	375115	375774	-1	Protein involved in outer spore wall assembly	Protein involved in outer spore wall assembly; likely involved directly in dityrosine layer assembly; induced during sporulation; repressed during vegetative growth by Sum1p and Hst1p; sequence similar to adjacent ORF, IRC18/YJL037W, and the irc18 loh1 double mutant exhibits reduced dityrosine fluorescence relative to the single mutants; SWAT-GFP and mCherry fusion proteins localize to the cytosol; proposed role in maintenance of genome integrity
S000003578	YJL042W	MHP1	MAP-Homologous Protein	361249	365445	1	Microtubule-associated protein involved in microtubule organization	Microtubule-associated protein involved in microtubule organization; involved in assembly and stabilization of microtubules; overproduction results in cell cycle arrest at G2 phase; similar to Drosophila protein MAP and to mammalian MAP4 proteins
S000003579	YJL043W	""	""	360130	360903	1	Putative protein of unknown function	Putative protein of unknown function; YJL043W is a non-essential gene
S000003580	YJL044C	GYP6	Gtpase-activating protein of Ypt6 Protein	358300	359676	-1	GTPase-activating protein (GAP) for yeast Rab family member Ypt6p	GTPase-activating protein (GAP) for yeast Rab family member Ypt6p; involved in vesicle mediated protein transport
S000003581	YJL045W	SDH9	Succinate DeHydrogenase	356024	357928	1	Minor succinate dehydrogenase isozyme	Minor succinate dehydrogenase isozyme; participates in oxidation of succinate and transfer of electrons to ubiquinone; induced during the diauxic shift in a Cat8p-dependent manner; YJL045W has a paralog, SDH1, that arose from the whole genome duplication
S000003582	YJL046W	AIM22	Altered Inheritance rate of Mitochondria	352386	353615	1	Lipoate-protein ligase	Lipoate-protein ligase; octanoyl-CoA: protein transferase required along with Lip2 and Lip5 for lipoylation of Lat1p and Kgd2p; similar to E. coli LplA; null mutant displays reduced frequency of mitochondrial genome loss
S000003583	YJL047C	RTT101	Regulator of Ty1 Transposition	349580	352108	-1	Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex	Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex; role in anaphase progression; Rtt101p-Mms22p ligase associates with replisome complex during S phase via Ctf4p; required for recovery after DSB repair; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p
S000003584	YJL048C	UBX6	UBiquitin regulatory X	347447	348637	-1	UBX (ubiquitin regulatory X) domain-containing protein	UBX (ubiquitin regulatory X) domain-containing protein; interacts with Cdc48p, transcription is repressed when cells are grown in media containing inositol and choline; UBX6 has a paralog, UBX7, that arose from the whole genome duplication
S000003585	YJL049W	CHM7	CHarged Multivesicular body protein	345973	347325	1	Phosphatidic acid (PA)-binding nuclear envelope (NE)-specific ESCRT	Phosphatidic acid (PA)-binding nuclear envelope (NE)-specific ESCRT; homolog of human CHMP7, localizes to the endoplasmic reticulum presumably as part of ESCRT-III like complex
S000003586	YJL050W	MTR4	Mrna TRansport	342522	345743	1	RNA duplex-sensing translocase	RNA duplex-sensing translocase; ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing
S000003587	YJL051W	IRC8	Increased Recombination Centers	339787	342255	1	Bud tip localized protein of unknown function	Bud tip localized protein of unknown function; mRNA is targeted to the bud by a She2p dependent transport system; mRNA is cell cycle regulated via Fkh2p, peaking in G2/M phase; null mutant displays increased levels of spontaneous Rad52p foc
S000003588	YJL052W	TDH1	Triose-phosphate DeHydrogenase	338271	339269	1	Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), isozyme 1	Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress; GAPDH-derived antimicrobial peptides secreted by S. cerevisiae are active against a wide variety of wine-related yeasts and bacteria
S000003589	YJL053W	PEP8	carboxyPEPtidase Y-deficient	335898	337037	1	Vacuolar protein component of the retromer	Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress
S000003590	YJL054W	TIM54	Translocase of the Inner Mitochondrial membrane	334265	335701	1	Component of the mitochondrial TIM22 complex	Component of the mitochondrial TIM22 complex; involved in insertion of polytopic proteins into the inner membrane
S000003591	YJL055W	LOG1	LOnely Guy	333357	334094	1	Putative protein of unknown function	Putative protein of unknown function; functions together with HAM1 to mediate resistance to 5-FU; specifically reduces the incorporation of 5-FU into RNA, without affecting uptake or incorporation of uracil into RNA; proposed to be involved in the metabolism of purine and pyrimidine base analogues; deletion mutants are sensitive to HAP and AHA
S000003592	YJL056C	ZAP1	Zinc-responsive Activator Protein	330431	333073	-1	Zinc-regulated transcription factor	Zinc-regulated transcription factor; binds to zinc-responsive promoters to induce transcription of certain genes in presence of zinc, represses other genes in low zinc; regulates its own transcription; contains seven zinc-finger domains
S000003593	YJL057C	IKS1	""	328118	330121	-1	Protein kinase of unknown cellular role	Protein kinase of unknown cellular role; putative serine/threonine kinase; expression is induced during mild heat stress; deletion mutants are hypersensitive to copper sulphate and resistant to sorbate; interacts with an N-terminal fragment of Sst2p
S000003594	YJL058C	BIT61	Binding partner of Tor2p	326242	327873	-1	Subunit of TORC2, a regulator of plasma membrane (PM) homeostasis	Subunit of TORC2, a regulator of plasma membrane (PM) homeostasis; TORC2 also regulates actin cytoskeletal dynamics during polarized growth and cell wall integrity; interacts with Slm1p and Slm2p, homologous PH domain-containing TORC2 substrates; PM retention is dependent on Avo3p; BIT61 has a paralog, BIT2, that arose from the whole genome duplication
S000003595	YJL059W	YHC3	Yeast Homolog of human Cln3	324964	326190	1	Protein required for the ATP-dependent transport of arginine	Protein required for the ATP-dependent transport of arginine; vacuolar membrane protein; involved in the ATP-dependent transport of arginine into the vacuole and possibly in balancing ion homeostasis; human homolog CLN3 involved in Batten disease (juvenile onset neuronal ceroid lipofuscinosis) can complement yeast null mutant
S000003596	YJL060W	BNA3	Biosynthesis of Nicotinic Acid	323386	324720	1	Kynurenine aminotransferase	Kynurenine aminotransferase; catalyzes formation of kynurenic acid from kynurenine; potential Cdc28p substrate
S000003597	YJL061W	NUP82	NUclear Pore	320016	322157	1	Linker nucleoporin component of the nuclear pore complex (NPC)	Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p); relocalizes to the cytosol in response to hypoxia
S000003598	YJL062W	LAS21	Local Anestheticum Sensitive	317284	319776	1	Mannose-ethanolamine phosphotransferase	Mannose-ethanolamine phosphotransferase; involved in biosynthesis of the glycosylphosphatidylinositol (GPI) core structure, catalyzing the addition of a side chain ethanolamine phosphate to the alpha1,6-linked second mannose residue of the GPI lipid precursor;  integral component of endoplasmic reticulum membrane; mutations affect cell wall integrity; homolog of human PIGG
S000003599	YJL063C	MRPL8	Mitochondrial Ribosomal Protein, Large subunit	315759	316475	-1	Mitochondrial ribosomal protein of the large subunit	Mitochondrial ribosomal protein of the large subunit
S000003600	YJL064W	""	""	315175	315570	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified gene YJL065C/DLS1
S000003601	YJL065C	DLS1	Dpb3-Like Subunit of ISW2/yCHRAC complex	315054	315557	-1	Subunit of ISW2/yCHRAC chromatin accessibility complex	Subunit of ISW2/yCHRAC chromatin accessibility complex; ISW2/yCHRAC also includes Itc1p, Isw2p, and Dpb4p; involved in inheritance of telomeric silencing; DLS1 has a paralog, DPB3, that arose from the whole genome duplication
S000003602	YJL066C	MPM1	Mitochondrial Peculiar Membrane protein	314114	314872	-1	Mitochondrial intermembrane space protein of unknown function	Mitochondrial intermembrane space protein of unknown function
S000003603	YJL067W	""	""	314084	314434	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000003604	YJL068C	""	""	313016	313915	-1	Esterase that can function as an S-formylglutathione hydrolase	Esterase that can function as an S-formylglutathione hydrolase; non-essential intracellular esterase; may be involved in the detoxification of formaldehyde, which can be metabolized to S-formylglutathione; similar to human esterase D
S000003606	YJL070C	""	""	307971	310637	-1	Putative metallo-dependent hydrolase superfamily protein	Putative metallo-dependent hydrolase superfamily protein; similar to AMP deaminases but lacks key catalytic residues and does not rescue purine nucleotide metabolic defect of quadruple aah1 ade8 amd1 his1 mutant; may regulate purine nucleotide homeostasis as overexpression in an AMD1 strain grown in adenine results in greatly reduced GDP and GTP intracellular levels; not an essential gene; YJL070C has a paralog, YBR284W, that arose from the whole genome duplication
S000003607	YJL071W	ARG2	ARGinine requiring	306132	307856	1	Acetylglutamate synthase (glutamate N-acetyltransferase)	Acetylglutamate synthase (glutamate N-acetyltransferase); mitochondrial enzyme that catalyzes the first step in the biosynthesis of the arginine precursor ornithine; forms a complex with Arg5,6p
S000003608	YJL072C	PSF2	Partner of Sld Five	305221	305862	-1	Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p)	Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p); complex is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
S000003609	YJL073W	JEM1	DnaJ-like protein of the ER Membrane	303181	305118	1	DnaJ-like chaperone required for nuclear membrane fusion during mating	DnaJ-like chaperone required for nuclear membrane fusion during mating; localizes to the ER membrane; exhibits genetic interactions with KAR2
S000003610	YJL074C	SMC3	Stability of MiniChromosomes	299157	302849	-1	Subunit of the multiprotein cohesin complex	Subunit of the multiprotein cohesin complex; required for sister chromatid cohesion in mitotic cells; also required, with Rec8p, for cohesion and recombination during meiosis; phylogenetically conserved SMC chromosomal ATPase family member
S000003611	YJL075C	APQ13	""	298460	298876	-1	Putative protein of unknown function	Putative protein of unknown function; not conserved in closely related Saccharomyces species; 85% of ORF overlaps verified gene NET1, but does not share all phenotypes; null mutant is slow-growing, sensitive to heat, sorbate, and other various chemicals
S000003612	YJL076W	NET1	Nucleolar silencing Establishing factor and Telophase regulator	295245	298814	1	Core subunit of the RENT complex	Core subunit of the RENT complex; involved in nucleolar silencing and telophase exit; stimulates transcription by RNA polymerase I and regulates nucleolar structure; NET1 has a paralog, TOF2, that arose from the whole genome duplication
S000003613	YJL077C	ICS3	Increased Copper Sensitivity	294666	295061	-1	Protein with a role in copper homeostasis	Protein with a role in copper homeostasis; possible role in vacuolar sorting and processing of secretory proteins; null mutants are hypersensitive to sortin2
S000003614	YJL078C	PRY3	Pathogen Related in Yeast	291336	293981	-1	GPI-anchored cell wall protein involved in export of sterols	GPI-anchored cell wall protein involved in export of sterols; redundant role with PRY1 and PRY2 in the export of free fatty acids; role in mating efficiency; expression of full-length transcript is daughter cell-specific; in response to alpha factor, a short transcript starting at +452 is expressed and the long form is repressed by Ste12p; member of the CAP protein superfamily (cysteine-rich secretory proteins (CRISP), antigen 5, and pathogenesis related 1 proteins)
S000003615	YJL079C	PRY1	Pathogen Related in Yeast	289875	290774	-1	Lipid binding protein	Lipid binding protein; binds to both free fatty acids with preference for saturated long-chain fatty acids and to sterols; contains two independent lipid-binding sites; involved in the export of free fatty acids and acetylated sterols; export functions are redundant with PRY2, and PRY3; important for survival of cells that accumulate free fatty acids; involved in detoxification  of hydrophobic compounds; secreted glycoprotein and member of the CAP/SCP protein superfamily
S000003616	YJL080C	SCP160	S. cerevisiae protein involved in the Control of Ploidy	285558	289226	-1	Essential RNA-binding G protein effector of mating response pathway	Essential RNA-binding G protein effector of mating response pathway; ligand-activated RNA-binding protein that delivers RNAs involved in polarization and perpetualizing mating signal to shmoo tip during pheromone signaling; Scp160p-mediated RNA trafficking essential for chemotropism and successful mating; mainly associated with nuclear envelope and ER, interacts in mRNA-dependent manner with translating ribosomes via multiple KH domains, similar to vertebrate vigilins
S000003617	YJL081C	ARP4	Actin-Related Protein	283802	285271	-1	Nuclear actin-related protein involved in chromatin remodeling	Nuclear actin-related protein involved in chromatin remodeling; component of chromatin-remodeling enzyme complexes
S000003618	YJL082W	IML2	Increased Minichromosome Loss	281185	283380	1	Protein required for clearance of inclusion bodies	Protein required for clearance of inclusion bodies; localizes to the inclusion bodies formed under protein misfolding stress; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress; IML2 has a paralog, YKR018C, that arose from the whole genome duplication
S000003619	YJL083W	TAX4	""	278841	280655	1	EH domain-containing protein	EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; TAX4 has a paralog, IRS4, that arose from the whole genome duplication
S000003620	YJL084C	ALY2	Arrestin-Like Yeast protein	274862	278002	-1	Alpha arrestin, Ub-ligase adaptor for Rsp5p	Alpha arrestin, Ub-ligase adaptor for Rsp5p; controls nutrient limitation-mediated endosomal recycling of the Gap1p amino acid permease; PY motif-containing adaptor involved in substrate-induced, Rsp5p-mediated ubiquitination and endocytosis of select plasma membrane localized amino acid transporters, such as Dip5p and Put4p; phosphorylated by Npr1p and by the Pcl7p-Pho85p cyclin-CDK complex; interacts with AP-1 subunit Apl4p
S000003621	YJL085W	EXO70	EXOcyst	272827	274698	1	Subunit of the exocyst complex	Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress
S000003623	YJL087C	TRL1	tRNA Ligase	270002	272485	-1	tRNA ligase	tRNA ligase; required for tRNA splicing and for both splicing and translation of HAC1 mRNA in the UPR; has phosphodiesterase, polynucleotide kinase, and ligase activities; involved in turnover of tRNA introns; localized at the inner nuclear envelope and cytoplasm
S000003624	YJL088W	ARG3	ARGinine requiring	268799	269815	1	Ornithine carbamoyltransferase	Ornithine carbamoyltransferase; also known as carbamoylphosphate:L-ornithine carbamoyltransferase; catalyzes the biosynthesis of the arginine precursor citrulline
S000003625	YJL089W	SIP4	SNF1-Interacting Protein	265926	268415	1	C6 zinc cluster transcriptional activator	C6 zinc cluster transcriptional activator; binds to the carbon source-responsive element (CSRE) of gluconeogenic genes; involved in the positive regulation of gluconeogenesis; regulated by Snf1p protein kinase; localized to the nucleus
S000003626	YJL090C	DPB11	DNA Polymerase B (II)	262757	265051	-1	DNA replication initiation protein	DNA replication initiation protein; loads DNA pol epsilon onto pre-replication complexes at origins; checkpoint sensor recruited to stalled replication forks by the checkpoint clamp complex where it activates Mec1p; along with Rfa1p, binds to ultrafine anaphase bridges in mitotic cells and prevents accumulation of chromatin bridges by stimulating the Mec1p kinase and suppressing homologous recombination; ortholog of human TopBP1; forms nuclear foci upon DNA replication stress
S000003627	YJL091C	GWT1	GPI-anchored Wall protein Transfer	261080	262552	-1	Protein involved in the inositol acylation of GlcN-PI	Protein involved in the inositol acylation of GlcN-PI; the inositol acylation of glucosaminyl phosphatidylinositol (GlcN-PI) forms glucosaminyl(acyl)phosphatidylinositol (GlcN(acyl)PI), an intermediate in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors
S000003628	YJL092W	SRS2	Suppressor of Rad Six	257423	260947	1	DNA helicase and DNA-dependent ATPase	DNA helicase and DNA-dependent ATPase; role in DNA repair and checkpoint recovery, in the proper timing of commitment to meiotic recombination and the Meiosis I to II transition; blocks trinucleotide repeat expansion; affects genome stability; disassembles Rad51p nucleoprotein filaments during meiotic recombination; stimulates Mus81p-Mms4p endonuclease activity independent of catalytic activity; ATPase and ssDNA translocating motor activities inhibited by Dmc1p; functional homolog of human RTEL1
S000003629	YJL093C	TOK1	""	254737	256812	-1	Outward-rectifier potassium channel of the plasma membrane	Outward-rectifier potassium channel of the plasma membrane; has two pore domains in tandem, each of which forms a functional channel permeable to potassium; carboxy tail functions to prevent inner gate closures; target of K1 toxin
S000003630	YJL094C	KHA1	K/H ion Antiporter	251821	254442	-1	Putative K+/H+ antiporter	Putative K+/H+ antiporter; involved in intracellular cation homeostasis; promotes copper binding to Fet3p multicopper oxidase; localized to Golgi vesicles and detected in highly purified mitochondria in high-throughput studies
S000003631	YJL095W	BCK1	Bypass of C Kinase	247255	251691	1	MAPKKK acting in the protein kinase C signaling pathway	MAPKKK acting in the protein kinase C signaling pathway; the kinase C signaling pathway controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p; MAPKKK is an acronym for mitogen-activated protein (MAP) kinase kinase kinase
S000003632	YJL096W	MRPL49	Mitochondrial Ribosomal Protein, Large subunit	246490	246975	1	Mitochondrial ribosomal protein of the large subunit	Mitochondrial ribosomal protein of the large subunit
S000003633	YJL097W	PHS1	PTPLA Homolog involved in Sphingolipid biosynthesis	245591	246244	1	Essential 3-hydroxyacyl-CoA dehydratase of the ER membrane	Essential 3-hydroxyacyl-CoA dehydratase of the ER membrane; involved in elongation of very long-chain fatty acids; evolutionarily conserved, similar to mammalian PTPLA and PTPLB; involved in sphingolipid biosynthesis and protein trafficking
S000003634	YJL098W	SAP185	Sit4 Associated Protein	242082	245258	1	Protein that forms a complex with the Sit4p protein phosphatase	Protein that forms a complex with the Sit4p protein phosphatase; required for Sit4p function; member of a family of similar proteins including Sap4p, Sap155p, and Sap190p; SAP185 has a paralog, SAP190, that arose from the whole genome duplication
S000003635	YJL099W	CHS6	CHitin Synthase-related	239414	241654	1	Member of the ChAPs (Chs5p-Arf1p-binding proteins) family	Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; primary component of the Chs5/6 complex that binds directly to membranes; CHS6 has a paralog, BCH2, that arose from the whole genome duplication
S000003636	YJL100W	LSB6	Las Seventeen Binding protein	237263	239086	1	Type II phosphatidylinositol 4-kinase	Type II phosphatidylinositol 4-kinase; binds Las17p, a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization
S000003637	YJL101C	GSH1	glutathione (GSH)	234320	236356	-1	Gamma glutamylcysteine synthetase	Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress
S000003638	YJL102W	MEF2	Mitochondrial Elongation Factor	231301	233760	1	Mitochondrial elongation factor involved in translational elongation	Mitochondrial elongation factor involved in translational elongation
S000003641	YJL105W	SET4	SET domain-containing	225055	226737	1	Chromatin-associated protein involved in control of stress response	Chromatin-associated protein involved in control of stress response; regulates response to oxidative stress; involved in control of gene repression in subtelomeric chromatin; contains a SET domain; SET4 has a paralog, SET3, that arose from the whole genome duplication
S000003642	YJL106W	IME2	Inducer of MEiosis	221390	223327	1	Serine/threonine protein kinase involved in activation of meiosis	Serine/threonine protein kinase involved in activation of meiosis; associates with Ime1p and mediates its stability, activates Ndt80p; IME2 expression is positively regulated by Ime1p; human CDK2 can complement ime2 null mutant
S000003643	YJL107C	""	""	218853	220016	-1	Putative protein of unknown function	Putative protein of unknown function; expression is induced by activation of the HOG1 mitogen-activated signaling pathway and this induction is Hog1p/Pbs2p dependent; YJL107C and adjacent ORF, YJL108C are merged in related fungi
S000003644	YJL108C	PRM10	Pheromone-Regulated Membrane protein	217705	218856	-1	Pheromone-regulated protein	Pheromone-regulated protein; proposed to be involved in mating; predicted to have 5 transmembrane segments; induced by treatment with 8-methoxypsoralen and UVA irradiation
S000003645	YJL109C	UTP10	U Three Protein	212000	217309	-1	Nucleolar protein	Nucleolar protein; component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA; mutant has increased aneuploidy tolerance
S000003646	YJL110C	GZF3	Gata Zinc Finger protein	209922	211577	-1	GATA zinc finger protein	GATA zinc finger protein; negatively regulates nitrogen catabolic gene expression by competing with Gat1p for GATA site binding; function requires a repressive carbon source; dimerizes with Dal80p and binds to Tor1p; GZF3 has a paralog, DAL80, that arose from the whole genome duplication
S000003648	YJL112W	MDV1	Mitochondrial DiVision	205305	207449	1	Peripheral protein of cytosolic face of mitochondrial outer membrane	Peripheral protein of cytosolic face of mitochondrial outer membrane; required for mitochondrial fission; interacts with Fis1p and with the self-assembled oligomeric form of the dynamin-related GTPase Dnm1p; contains WD repeats; MDV1 has a paralog, CAF4, that arose from the whole genome duplication
S000003651	YJL115W	ASF1	Anti-Silencing Function	196287	197126	1	Nucleosome assembly factor	Nucleosome assembly factor; involved in chromatin assembly, disassembly; required for recovery after DSB repair; role in H3K56 acetylation required for expression homeostasis, buffering mRNA synthesis rate against gene dosage changes in S phase; anti-silencing protein, derepresses silent loci when overexpressed; role in regulating Ty1 transposition; relocalizes to cytosol under hypoxia; growth defect of asf1 null is functionally complemented by either human ASF1A or ASF1B
S000003652	YJL116C	NCA3	Nuclear Control of ATPase	193860	194873	-1	Protein involved in mitochondrion organization	Protein involved in mitochondrion organization; functions with Nca2p to regulate mitochondrial expression of subunits 6 (Atp6p) and 8 (Atp8p) of the Fo-F1 ATP synthase; SWAT-GFP, seamless-GFP and mCherry fusion proteins localize to the vacuole; member of the SUN family; expression induced in cells treated with the mycotoxin patulin; NCA3 has a paralog, UTH1, that arose from the whole genome duplication
S000003653	YJL117W	PHO86	PHOsphate metabolism	192531	193466	1	Endoplasmic reticulum (ER) resident protein	Endoplasmic reticulum (ER) resident protein; required for ER exit of the high-affinity phosphate transporter Pho84p, specifically required for packaging of Pho84p into COPII vesicles; protein abundance increases in response to DNA replication stress
S000003654	YJL118W	""	""	191639	192298	1	Protein of unknown function	Protein of unknown function; may interact with ribosomes, based on co-purification experiments; YJL118W is a non-essential gene; deletion enhances the toxicity of heterologously expressed human alpha-synuclein
S000003655	YJL119C	""	""	191572	191895	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000003656	YJL120W	""	""	191022	191345	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL121C/RPE1; deletion confers sensitivity to GSAO
S000003657	YJL121C	RPE1	Ribulose 5-Phosphate Epimerase	190374	191090	-1	D-ribulose-5-phosphate 3-epimerase	D-ribulose-5-phosphate 3-epimerase; catalyzes a reaction in the non-oxidative part of the pentose-phosphate pathway; mutants are sensitive to oxidative stress
S000003658	YJL122W	ALB1	Arx1 Little Brother	189716	190243	1	Shuttling pre-60S factor	Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit; interacts directly with Arx1p; responsible for Tif6p recycling defects in absence of Rei1p
S000003659	YJL123C	MTC1	Maintenance of Telomere Capping	188004	189440	-1	Protein of unknown function that may interact with ribosomes	Protein of unknown function that may interact with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to COPI-coated vesicles (early Golgi); mtc1 is synthetically lethal with cdc13-1
S000003660	YJL124C	LSM1	Like SM	187126	187644	-1	Lsm (Like Sm) protein	Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; also enters the nucleus and positively regulates transcription initiation; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; binds to mRNAs under glucose starvation, most often in the 3' UTR; forms cytoplasmic foci upon DNA replication stress
S000003661	YJL125C	GCD14	General Control Nonderepressible	185528	186679	-1	Subunit of tRNA (1-methyladenosine) methyltransferase	Subunit of tRNA (1-methyladenosine) methyltransferase; required, along with Gcd10p, for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression
S000003662	YJL126W	NIT2	NITrilase superfamily	184501	185424	1	Nit protein	Nit protein; one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member
S000003663	YJL127C	SPT10	SuPpressor of Ty	182298	184220	-1	Histone H3 acetylase with a role in transcriptional regulation	Histone H3 acetylase with a role in transcriptional regulation; sequence-specific activator of histone genes, binds specifically and cooperatively to pairs of UAS elements in core histone promoters, functions at or near TATA box; involved in S phase-specific acetylation of H3K56 at histone promoters, which is required for recruitment of SWI/SNF nucleosome remodeling complex and subsequent transcription
S000003664	YJL128C	PBS2	Polymyxin B Sensitivity	178097	180103	-1	MAP kinase kinase of the HOG signaling pathway	MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition
S000003665	YJL129C	TRK1	TRansport of potassium (K)	173599	177306	-1	Component of the Trk1p-Trk2p potassium transport system	Component of the Trk1p-Trk2p potassium transport system; 180 kDa high affinity potassium transporter; phosphorylated in vivo and interacts physically with the phosphatase Ppz1p, suggesting Trk1p acitivy is regulated by phosphorylation; TRK1 has a paralog, TRK2, that arose from the whole genome duplication
S000003666	YJL130C	URA2	URAcil requiring	165723	172367	-1	Bifunctional carbamoylphosphate synthetase/aspartate transcarbamylase	Bifunctional carbamoylphosphate synthetase/aspartate transcarbamylase; catalyzes the first two enzymatic steps in the de novo biosynthesis of pyrimidines; both activities are subject to feedback inhibition by UTP
S000003667	YJL131C	AIM23	Altered Inheritance rate of Mitochondria	164278	165348	-1	Mitochondrial translation initiation factor 3 (IF3, mIF3)	Mitochondrial translation initiation factor 3 (IF3, mIF3); evolutionarily conserved; interacts with mitochondrial ribosomal small subunit; binds to E. coli ribosomes in vitro; null mutant displays severe respiratory growth defect and elevated frequency of mitochondrial genome loss
S000003668	YJL132W	""	""	161914	164166	1	Putative protein of unknown function	Putative protein of unknown function; localizes to the membrane fraction; possible Zap1p-regulated target gene induced by zinc deficiency; YJL132W is a non-essential gene
S000003669	YJL133W	MRS3	Mitochondrial RNA Splicing	160619	161563	1	Iron transporter, mediates Fe2+ transport across inner mito membrane	Iron transporter, mediates Fe2+ transport across inner mito membrane; mitochondrial carrier family member; active under low-iron conditions; may transport other cations; MRS3 has a paralog, MRS4, that arose from the whole genome duplication
S000003670	YJL134W	LCB3	Long-Chain Base	158188	159417	1	Long-chain base-1-phosphate phosphatase	Long-chain base-1-phosphate phosphatase; specific for dihydrosphingosine-1-phosphate, regulates ceramide and long-chain base phosphates levels, involved in incorporation of exogenous long chain bases in sphingolipids; LCB3 has a paralog, YSR3, that arose from the whole genome duplication
S000003671	YJL135W	""	""	157877	158194	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL134W/LCB3
S000003672	YJL136C	RPS21B	Ribosomal Protein of the Small subunit	156550	157273	-1	Protein component of the small (40S) ribosomal subunit	Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S21, no bacterial homolog; RPS21B has a paralog, RPS21A, that arose from the whole genome duplication
S000003673	YJL137C	GLG2	Glycogenin-Like Gene	154985	156127	-1	Glycogenin glucosyltransferase	Glycogenin glucosyltransferase; self-glucosylating initiator of glycogen synthesis, also glucosylates n-dodecyl-beta-D-maltoside; similar to mammalian glycogenin; GLG2 has a paralog, GLG1, that arose from the whole genome duplication
S000003674	YJL138C	TIF2	Translation Initiation Factor	153504	154691	-1	Translation initiation factor eIF4A	Translation initiation factor eIF4A; DEAD-box RNA helicase that couples ATPase activity to mRNA binding and unwinding; ATP hydrolysis accelerates the recruitment of mRNA during 48S preinitiation complex (PIC) assembly; ATPase activity stimulated by either eIF4G1 or by the PIC in an eIF3g and eIFGi subunit-dependent manner, independent of eIF4E and eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) with eIF4E and eIF4G; protein abundance increases in response to DNA replication stress
S000003675	YJL139C	YUR1	Yeast Unknown Reading frame	151713	152999	-1	Mannosyltransferase involved in protein N-glycosylation	Mannosyltransferase involved in protein N-glycosylation; member of the KTR1 family; located in the Golgi apparatus; YUR1 has a paralog, KTR2, that arose from the whole genome duplication
S000003676	YJL140W	RPB4	RNA Polymerase B	150961	151626	1	RNA polymerase II subunit B32	RNA polymerase II subunit B32; forms dissociable heterodimer with Rpb7p; Rpb4/7 dissociates from RNAPII as Ser2 CTD phosphorylation increases; Rpb4/7 regulates cellular lifespan via mRNA decay process; involved in recruitment of 3'-end processing factors to transcribing RNAPII complex, export of mRNA to cytoplasm under stress conditions; also involved in translation initiation
S000003677	YJL141C	YAK1	Yet Another Kinase	147967	150390	-1	Serine-threonine protein kinase	Serine-threonine protein kinase; component of a glucose-sensing system that inhibits growth in response to glucose availability; upon nutrient deprivation Yak1p phosphorylates Pop2p to regulate mRNA deadenylation, the co-repressor Crf1p to inhibit transcription of ribosomal genes, and the stress-responsive transcription factors Hsf1p and Msn2p; nuclear localization negatively regulated by the Ras/PKA signaling pathway in the presence of glucose
S000003678	YJL142C	IRC9	Increased Recombination Centers	147819	148211	-1	Putative protein of unknown function	Putative protein of unknown function; partially overlaps verified gene YAK1/YJL141C but does not share all phenotypes; null mutant displays increased levels of spontaneous Rad52p foci, increased sporulation efficiency, and small defect in vacuolar fragmentation
S000003679	YJL143W	TIM17	Translocase of the Inner Mitochondrial membrane	147101	147577	1	Essential core component of the TIM23 protein translocase complex	Essential core component of the TIM23 protein translocase complex; forms the protein translocation path with Mgr2p, while Tim23p contributes to the architecture of the import channel; may link the import motor to the core translocase of the inner mitochondrial membrane (TIM23 complex)
S000003680	YJL144W	ROQ1	Regulator Of Quality control	146359	146673	1	Ub-ligase substrate-specificity factor	Ub-ligase substrate-specificity factor; proteolytically-cleaved form acts as a pseudosubstrate, binding and altering the substrate specificity of Ubr1p towards misfolded and native ER membrane and cytosolic proteins, as part of the stress-induced homeostatically regulated protein degradation (SHRED) pathway; hydrophilin essential during desiccation-rehydration; induced by osmotic stress, starvation and during stationary phase; protein abundance increases in response to DNA replication stress
S000003681	YJL145W	SFH5	Sec Fourteen Homolog	145160	146044	1	Unusual phosphatidylinositol transfer protein (PITP) that binds heme	Unusual phosphatidylinositol transfer protein (PITP) that binds heme; similar to Sec14p; founding member of conserved class of fungal hemoproteins; exhibits PI- but not PC-transfer activity; localizes to peripheral endoplasmic reticulum, cytosol, and microsomes; partially relocalizes to plasma membrane upon DNA replication stress
S000003682	YJL146W	IDS2	IME2-Dependent Signaling	143292	144701	1	Protein involved in modulation of Ime2p activity during meiosis	Protein involved in modulation of Ime2p activity during meiosis; appears to act indirectly to promote Ime2p-mediated late meiotic functions; found in growing cells and degraded during sporulation
S000003683	YJL147C	SMT1	Suppression of Mitochondrial Translation	141419	142567	-1	Translational repressor of the mitochondrial ATP6/8 mRNA	Translational repressor of the mitochondrial ATP6/8 mRNA; homozygous diploid deletion strain has a sporulation defect characterized by elevated dityrosine in the soluble fraction; expression induced by calcium shortage
S000003684	YJL148W	RPA34	RNA Polymerase A	140437	141138	1	RNA polymerase I subunit A34.5	RNA polymerase I subunit A34.5; essential for nucleolar assembly and for high polymerase loading rate; nucleolar localization depends on Rpa49p
S000003685	YJL149W	DAS1	Dst1-delta 6-Azauracil Sensitivity	137379	139370	1	Putative SCF ubiquitin ligase F-box protein	Putative SCF ubiquitin ligase F-box protein; interacts physically with both Cdc53p and Skp1 and genetically with CDC34; similar to putative F-box protein YDR131C
S000003686	YJL150W	""	""	137123	137425	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000003687	YJL151C	SNA3	Sensitivity to NA+	136372	136773	-1	Protein involved in efficient MVB sorting of proteins to the vacuole	Protein involved in efficient MVB sorting of proteins to the vacuole; may function as an RSP5 adapter protein for MVB cargos; integral membrane protein localized to vacuolar intralumenal vesicles
S000003688	YJL152W	""	""	136174	136533	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000003689	YJL153C	INO1	INOsitol requiring	134332	135933	-1	Inositol-3-phosphate synthase	Inositol-3-phosphate synthase; involved in synthesis of inositol phosphates and inositol-containing phospholipids; transcription is coregulated with other phospholipid biosynthetic genes by Ino2p and Ino4p, which bind the UASINO DNA element
S000003690	YJL154C	VPS35	Vacuolar Protein Sorting	131101	133935	-1	Endosomal subunit of membrane-associated retromer complex	Endosomal subunit of membrane-associated retromer complex; required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p; overexpression of wild-type human VPS35 or Parkinson's-associated vps35-D686N or vps35-P299S variants complements Ni2+ resistance and Cd2+ sensitivity of yeast vps35 null mutant
S000003691	YJL155C	FBP26	Fructose BisPhosphatase	129285	130643	-1	Fructose-2,6-bisphosphatase, required for glucose metabolism	Fructose-2,6-bisphosphatase, required for glucose metabolism; protein abundance increases in response to DNA replication stress
S000003693	YJL157C	FAR1	Factor ARrest	123836	126328	-1	CDK inhibitor and nuclear anchor	CDK inhibitor and nuclear anchor; during the cell cycle Far1p sequesters the GEF Cdc24p in the nucleus; phosphorylation by Cdc28p-Cln results in SCFCdc4 complex-mediated ubiquitin-dependent degradation, releasing Cdc24p for export and activation of GTPase Cdc42p; in response to pheromone, phosphorylation of Far1p by MAPK Fus3p results in association with, and inhibition of Cdc28p-Cln, as well as Msn5p mediated nuclear export of Far1p-Cdc24p, targeting Cdc24p to polarity sites
S000003694	YJL158C	CIS3	CIk1 Suppressing	122265	122948	-1	Mannose-containing glycoprotein constituent of the cell wall	Mannose-containing glycoprotein constituent of the cell wall; member of the PIR (proteins with internal repeats) family
S000003695	YJL159W	HSP150	Heat Shock Protein	120449	121690	1	O-mannosylated heat shock protein	O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication
S000003696	YJL160C	PIR5	Protein with Internal Repeats	117962	118825	-1	Putative protein of unknown function	Putative protein of unknown function; member of the PIR (Proteins with Internal Repeats) family of cell wall proteins; SWAT-GFP fusion protein localizes to the endoplasmic reticulum and vacuole, and mCherry fusion localizes to the vacuole; non-essential gene that is required for sporulation; mRNA is weakly cell cycle regulated, peaking in mitosis; YJL160C has a paralog, PIR1, that arose from the whole genome duplication
S000003697	YJL161W	FMP33	Found in Mitochondrial Proteome	117245	117787	1	Putative protein of unknown function	Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
S000003698	YJL162C	JJJ2	J-protein (Type III)	113878	115629	-1	Protein of unknown function	Protein of unknown function; contains a J-domain, which is a region with homology to the E. coli DnaJ protein
S000003699	YJL163C	""	""	111666	113333	-1	Putative protein of unknown function	Putative protein of unknown function
S000003700	YJL164C	TPK1	Takashi's Protein Kinase	109966	111159	-1	cAMP-dependent protein kinase catalytic subunit	cAMP-dependent protein kinase catalytic subunit; role in nutrient-stimulated growth via Ras-cAMP signaling; inhibited by regulatory subunit Bcy1p in cAMPs absence; phosphorylates and inhibits Whi3p to promote G1/S phase passage; phosphorylates Nej1p to regulate nonhomologous end joining (NHEJ); impairs mitochondrial import of pre-Tom40p via phosphorylation under non-respiratory conditions; partially redundant with Tpk2p and Tpk3p; human homolog PRKACB regulates NHEJ via NHEJ1 phosphorylation
S000003701	YJL165C	HAL5	HALotolerance	106894	109461	-1	Snf1p-related nutrient-responsive protein kinase	Snf1p-related nutrient-responsive protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; HAL5 has a paralog, KKQ8, that arose from the whole genome duplication
S000003702	YJL166W	QCR8	ubiQuinol-cytochrome C oxidoReductase	106434	106718	1	Subunit 8 of ubiquinol cytochrome-c reductase (Complex III)	Subunit 8 of ubiquinol cytochrome-c reductase (Complex III); Complex III is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p
S000003703	YJL167W	ERG20	ERGosterol biosynthesis	105014	106072	1	Farnesyl pyrophosphate synthetase	Farnesyl pyrophosphate synthetase; has both dimethylallyltranstransferase and geranyltranstransferase activities; catalyzes the formation of C15 farnesyl pyrophosphate units for isoprenoid and sterol biosynthesis
S000003704	YJL168C	SET2	SET domain-containing	102227	104428	-1	Histone methyltransferase with a role in transcriptional elongation	Histone methyltransferase with a role in transcriptional elongation; methylates H3 lysine 36 (H3K36), which suppresses incorporation of acetylated histones and signals for their deacetylation within transcribed genes; involved in transcription coupled-nucleotide excision repair; associates with C-terminal domain (CTD) of Rpo21p; H3K36me3 (trimethylation) requires Spt6p, proline 38 on H3, CTD of Rpo21p, Ctk1p, and C-terminal SRI domain of Ste2p; relocalizes to cytosol in response to hypoxia
S000003705	YJL169W	""	""	102099	102467	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL168C/SET2
S000003706	YJL170C	ASG7	a-Specific Gene	101150	101779	-1	Protein that regulates signaling from G protein beta subunit Ste4p	Protein that regulates signaling from G protein beta subunit Ste4p; contributes to relocalization of Ste4p within the cell; specific to a-cells and induced by alpha-factor
S000003707	YJL171C	TOH1	TOS One Homolog	99704	100894	-1	GPI-anchored cell wall protein of unknown function	GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress
S000003708	YJL172W	CPS1	CarboxyPeptidase yscS	97737	99467	1	Vacuolar carboxypeptidase S	Vacuolar carboxypeptidase S; targeted to vacuole via Vps10p-dependent endosomal vacuolar protein sorting pathway and via AP-3 pathway; expression induced under low-nitrogen conditions
S000003710	YJL174W	KRE9	Killer toxin REsistant	95092	95922	1	Glycoprotein involved in cell wall beta-glucan assembly	Glycoprotein involved in cell wall beta-glucan assembly; null mutation leads to severe growth defects, aberrant multibudded morphology, and mating defects
S000003711	YJL175W	""	""	94049	94561	1	Dubious open reading frame unlikely to encode a functional protein	Dubious open reading frame unlikely to encode a functional protein; deletion confers resistance to cisplatin, hypersensitivity to 5-fluorouracil, and growth defect at high pH with high calcium; overlaps gene for SWI3 transcription factor
S000003712	YJL176C	SWI3	SWItching deficient	92053	94530	-1	Subunit of the SWI/SNF chromatin remodeling complex	Subunit of the SWI/SNF chromatin remodeling complex; SWI/SNF regulates transcription by remodeling chromosomes; contains SANT domain that is required for SWI/SNF assembly; is essential for displacement of histone H2A-H2B dimers during ATP-dependent remodeling; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; relocates to the cytosol under hypoxic conditions
S000003713	YJL177W	RPL17B	Ribosomal Protein of the Large subunit	90786	91657	1	Ribosomal 60S subunit protein L17B	Ribosomal 60S subunit protein L17B; required for processing of 27SB pre-rRNA and formation of stable 66S assembly intermediates; homologous to mammalian ribosomal protein L17 and bacterial L22; RPL17B has a paralog, RPL17A, that arose from the whole genome duplication
S000003714	YJL178C	ATG27	AuTophaGy related	89282	90097	-1	Type I membrane protein involved in autophagy and the Cvt pathway	Type I membrane protein involved in autophagy and the Cvt pathway; targeted to vacuole via AP-3 pathway
S000003715	YJL179W	PFD1	PreFolDin	88787	89116	1	Subunit of heterohexameric prefoldin	Subunit of heterohexameric prefoldin; prefoldin binds cytosolic chaperonin and transfers target proteins to it; involved in the biogenesis of actin and of alpha- and gamma-tubulin; prefoldin complex also localizes to chromatin of actively transcribed genes in the nucleus and facilitates transcriptional elongation
S000003716	YJL180C	ATP12	ATP synthase	87583	88560	-1	Assembly factor for F1 sector of mitochondrial F1F0 ATP synthase	Assembly factor for F1 sector of mitochondrial F1F0 ATP synthase; conserved protein; required for assembly of alpha and beta subunits into F1 sector of mitochondrial F1F0 ATP synthase; human homolog ATPAF2 can complement yeast atp12 mutant; mutation of human homolog reduces active ATP synthase levels and is associated with the disorder ATPAF2 deficiency
S000003717	YJL181W	RBH1	Ran Binding domain Homolog	85660	87495	1	Putative protein of unknown function	Putative protein of unknown function; expression is cell-cycle regulated as shown by microarray analysis; potential regulatory target of Mbp1p, which binds to the YJL181W promoter region; contains a PH-like domain; RBH1 has a paralog, RBH2, that arose from the whole genome duplication
S000003718	YJL182C	""	""	85435	85752	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps uncharacterized ORF YJL181W
S000003719	YJL183W	MNN11	MaNNosyltransferase	84068	85336	1	Subunit of a Golgi mannosyltransferase complex	Subunit of a Golgi mannosyltransferase complex; this complex also contains Anp1p, Mnn9p, Mnn10p, and Hoc1p, and mediates elongation of the polysaccharide mannan backbone; has homology to Mnn10p
S000003720	YJL184W	GON7	""	83448	83819	1	Component of the EKC/KEOPS protein complex	Component of the EKC/KEOPS protein complex; EKC/KEOPS complex is required for t6A tRNA modification and telomeric TG1-3 recombination; may have role in transcription; implicated in osmotic stress response; other complex members are Kae1p, Cgi121p, Pcc1p, and Bud32p
S000003721	YJL185C	ATG36	AuTophaGy related	82095	82976	-1	Pex3p interacting protein, required for pexophagy	Pex3p interacting protein, required for pexophagy; interacts with Atg8p and Atg11p; mRNA is weakly cell cycle regulated, peaking in G2 phase; YJL185C is a non-essential gene
S000003722	YJL186W	MNN5	MaNNosyltransferase	80155	81915	1	Alpha-1,2-mannosyltransferase	Alpha-1,2-mannosyltransferase; responsible for addition of the second alpha-1,2-linked mannose of the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment
S000003723	YJL187C	SWE1	Saccharomyces WEe1	76803	79262	-1	Protein kinase that regulates the G2/M transition	Protein kinase that regulates the G2/M transition; negative regulator of the Cdc28p kinase; morphogenesis checkpoint kinase; positive regulator of sphingolipid biosynthesis via Orm2p; phosphorylates a tyrosine residue in the N-terminus of Hsp90 in a cell-cycle associated manner, thus modulating the ability of Hsp90 to chaperone a selected clientele; localizes to the nucleus and to the daughter side of the mother-bud neck; homolog of S. pombe Wee1p; potential Cdc28p substrate
S000003724	YJL188C	BUD19	BUD site selection	76202	76510	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay
S000003725	YJL189W	RPL39	Ribosomal Protein of the Large subunit	75933	76474	1	Ribosomal 60S subunit protein L39	Ribosomal 60S subunit protein L39; required for ribosome biogenesis; loss of both Rpl31p and Rpl39p confers lethality; also exhibits genetic interactions with SIS1 and PAB1; homologous to mammalian ribosomal protein L39, no bacterial homolog
S000003726	YJL190C	RPS22A	Ribosomal Protein of the Small subunit	74910	75302	-1	Protein component of the small (40S) ribosomal subunit	Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22A has a paralog, RPS22B, that arose from the whole genome duplication
S000003727	YJL191W	RPS14B	Ribosomal Protein of the Small subunit	73787	74611	1	Protein component of the small (40S) ribosomal subunit	Protein component of the small (40S) ribosomal subunit; required for ribosome assembly and 20S pre-rRNA processing; mutations confer cryptopleurine resistance; homologous to mammalian ribosomal protein S14 and bacterial S11; RPS14B has a paralog, RPS14A, that arose from the whole genome duplication
S000003728	YJL192C	SOP4	Suppressor Of Pma1-7	72710	73414	-1	ER-membrane protein	ER-membrane protein; subunit of evolutionarily conserved EMC (Endoplasmic Reticulum Membrane Complex) implicated in ERAD (ER-associated degradation) and proper assembly of multi-pass transmembrane (TM) proteins; EMC acts in yeast as an ER-mitochondria tether that interacts with outer membrane protein Tom5 of TOM (Translocase of the Mitochondrial Outer Membrane) complex; suppressor of pma1-7, deletion of SOP4 slows down export of wild-type Pma1p and Pma1-7 from the ER
S000003729	YJL193W	""	""	71366	72574	1	Putative protein of unknown function	Putative protein of unknown function; predicted to encode a triose phosphate transporter subfamily member based on phylogenetic analysis; similar to YOR307C/SLY41; deletion mutant has a respiratory growth defect
S000003730	YJL194W	CDC6	Cell Division Cycle	69338	70879	1	Essential ATP-binding protein required for DNA replication	Essential ATP-binding protein required for DNA replication; component of the pre-replicative complex (pre-RC) which requires ORC to associate with chromatin and is in turn required for Mcm2-7p DNA association; dual role as a mitotic cyclin-dependent kinase inhibitor, that binds to and inhibits Cdc28p/Clb2p along with Sic1p, contributing to mitotic exit; homologous to S. pombe Cdc18p; relocalizes from nucleus to cytoplasm upon DNA replication stress; degraded in response to plasma membrane stress
S000003731	YJL195C	""	""	69241	69942	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6
S000003732	YJL196C	ELO1	ELOngation defective	67850	68782	-1	Elongase I, medium-chain acyl elongase	Elongase I, medium-chain acyl elongase; catalyzes carboxy-terminal elongation of unsaturated C12-C16 fatty acyl-CoAs to C16-C18 fatty acids; ELO1 has a paralog, ELO2, that arose from the whole genome duplication
S000003733	YJL197W	UBP12	UBiquitin-specific Protease	63805	67569	1	Ubiquitin-specific protease	Ubiquitin-specific protease; cleaves ubiquitin from ubiquitinated proteins; present in the nucleus and cytoplasm
S000003734	YJL198W	PHO90	PHOsphate metabolism	60844	63489	1	Low-affinity phosphate transporter	Low-affinity phosphate transporter; acts upstream of Pho81p in regulation of the PHO pathway; deletion of pho84, pho87, pho89, pho90, and pho91 causes synthetic lethality; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth; PHO90 has a paralog, PHO87, that arose from the whole genome duplication
S000003735	YJL199C	MBB1	""	59856	60182	-1	Protein of unknown function	Protein of unknown function; conserved among S. cerevisiae strains, not conserved in closely related Saccharomyces species; protein detected via large-scale protein-protein interaction studies and enrichment-based proteogenomics; YJL199C is not an essential gene
S000003736	YJL200C	ACO2	ACOnitase	56444	58813	-1	Putative mitochondrial aconitase isozyme	Putative mitochondrial aconitase isozyme; similarity to Aco1p, an aconitase required for the TCA cycle; expression induced during growth on glucose, by amino acid starvation via Gcn4p, and repressed on ethanol
S000003737	YJL201W	ECM25	ExtraCellular Mutant	54379	56178	1	Endocytic site protein required for stress-induced cell elongation	Endocytic site protein required for stress-induced cell elongation; may link the polarization signal with the endocytic machinery to facilitate adaptive morphogenesis under conditions of stress; interacts with the polarity regulator Cdc42p, the SH3 domains of actin patch components and actin filaments; contains a Rho-GAP domain lacking the catalytic arginine finger, PXXP motifs and an xACT (F-actin binding) motif; promoter contains a consensus binding sequence for transcription factor Abf1p
S000003739	YJL203W	PRP21	Pre-mRNA Processing	53341	54183	1	Subunit of the SF3a splicing factor complex	Subunit of the SF3a splicing factor complex; required for spliceosome assembly
S000003740	YJL204C	RCY1	ReCYcling	50629	53151	-1	F-box protein involved in recycling endocytosed proteins	F-box protein involved in recycling endocytosed proteins; involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth; direct interaction with C-terminal cytoplasmic region of Drs2p plays an important role for Drs2p function in endocytic recycling pathway; required for turnover of histone H3 variant Cse4p
S000003741	YJL206C	""	""	47659	49935	-1	Putative protein of unknown function	Putative protein of unknown function; similar to transcriptional regulators from the Zn[2]-Cys[6] binuclear cluster protein family; mRNA is weakly cell cycle regulated, peaking in S phase; induced rapidly upon MMS treatment
S000003742	YJL205C	NCE101	NonClassical Export	50139	50443	-1	Protein of unknown function	Protein of unknown function; involved in secretion of proteins that lack classical secretory signal sequences; SWAT-GFP and mCherry fusion proteins localize to the cytosol
S000003743	YJL207C	LAA1	Large AP-1 Accessory	41389	47433	-1	AP-1 accessory protein	AP-1 accessory protein; colocalizes with clathrin to the late-Golgi apparatus; involved in TGN-endosome transport; physically interacts with AP-1; similar to the mammalian p200; may interact with ribosomes; YJL207C is a non-essential gene
S000003744	YJL208C	NUC1	NUClease	40194	41183	-1	Major mitochondrial nuclease	Major mitochondrial nuclease; has RNAse and DNA endo- and exonucleolytic activities; roles in mitochondrial recombination, apoptosis and maintenance of polyploidy; involved in fragmentation of genomic DNA during PND (programmed nuclear destruction); role in preventing L-A mycovirus pathogenesis; encodes ortholog of mammalian endoG
S000003745	YJL209W	CBP1	Cytochrome B mRNA Processing	38005	39969	1	Mitochondrial protein, regulator of COB mRNA stability and translation	Mitochondrial protein, regulator of COB mRNA stability and translation; interacts with the 5'-untranslated region of the COB mRNA; found in a complex at the inner membrane along with Pet309p; localizes to mitochondrial foci upon DNA replication stress
S000003746	YJL210W	PEX2	PEroXin	36919	37734	1	RING-finger peroxin and E3 ubiquitin ligase	RING-finger peroxin and E3 ubiquitin ligase; peroxisomal membrane protein with a C-terminal zinc-binding RING domain, forms translocation subcomplex with Pex10p and Pex12p which functions in peroxisomal matrix protein import
S000003747	YJL211C	""	""	36757	37200	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL210W/PEX2
S000003748	YJL212C	OPT1	OligoPeptide Transporter	33850	36249	-1	Proton-coupled oligopeptide transporter of the plasma membrane	Proton-coupled oligopeptide transporter of the plasma membrane; also transports glutathione and phytochelatin; member of the OPT family
S000003749	YJL213W	""	""	32163	33158	1	Protein of unknown function that may interact with ribosomes	Protein of unknown function that may interact with ribosomes; periodically expressed during the yeast metabolic cycle; phosphorylated in vitro by the mitotic exit network (MEN) kinase complex, Dbf2p/Mob1p
S000003750	YJL214W	HXT8	HeXose Transporter	26887	28596	1	Protein of unknown function with similarity to hexose transporters	Protein of unknown function with similarity to hexose transporters; expression is induced by low levels of glucose and repressed by high levels of glucose
S000003751	YJL215C	""	""	26412	26771	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000003752	YJL216C	IMA5	IsoMAltase	24341	26086	-1	Alpha-glucosidase	Alpha-glucosidase; specificity for isomaltose, maltose, and palatinose, but not alpha-methylglucoside; most distant member of the IMA isomaltase family, but with similar catalytic properties as Ima1p and Ima2p; not required for isomaltose utilization, but Ima5p overexpression allows the ima1 null mutant to grow on isomaltose; can cleave alpha-1,3 linkage of nigerose and turanose and alpha-1,5 linkage of leucrose and is very sensitive to temperature in vitro
S000003753	YJL217W	REE1	REgulation of Enolase	23133	23729	1	Cytoplasmic protein involved in the regulation of enolase (ENO1)	Cytoplasmic protein involved in the regulation of enolase (ENO1); mRNA expression is induced by calcium shortage, copper deficiency (via Mac1p) and the presence of galactose (via Gal4p); mRNA expression is also regulated by the cell cycle
S000003754	YJL218W	""	""	21973	22563	1	Mitochondrial protein, putative acetyltransferase	Mitochondrial protein, putative acetyltransferase; similar to bacterial galactoside O-acetyltransferases; induced by oleate in OAF1/PIP2-dependent manner; promoter contains oleate response element consensus sequence; mutation decreases salt tolerance; non-essential gene
S000003761	YJR001W	AVT1	Amino acid Vacuolar Transport	436802	438610	1	Vacuolar transporter	Vacuolar transporter; imports large neutral amino acids into vacuole; targeted to vacuole via AP-3 pathway; member of family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
S000003763	YJR003C	MRX12	Mitochondrial oRganization of gene eXpression (MIOREX)	440994	442553	-1	Protein that associates with mitochondrial ribosome	Protein that associates with mitochondrial ribosome; detected in highly purified mitochondria in high-throughput studies; predicted to be involved in ribosome biogenesis
S000003764	YJR004C	SAG1	Sexual AGglutination	442909	444861	-1	Alpha-agglutinin of alpha-cells	Alpha-agglutinin of alpha-cells; binds to Aga1p during agglutination, N-terminal half is homologous to the immunoglobulin superfamily and contains binding site for a-agglutinin, C-terminal half is highly glycosylated and contains GPI anchor
S000003765	YJR005W	APL1	clathrin Adaptor Protein complex Large chain	445923	448025	1	Beta-adaptin	Beta-adaptin; large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport; similar to mammalian beta-chain of the clathrin associated protein complex
S000003767	YJR007W	SUI2	SUppressor of Initiator codon	451020	451934	1	Alpha subunit of the translation initiation factor eIF2	Alpha subunit of the translation initiation factor eIF2; eIF2 is involved in identification of the start codon; phosphorylation of Ser51 is required for regulation of translation by inhibiting the exchange of GDP for GTP; protein abundance increases in response to DNA replication stress
S000003768	YJR008W	MHO1	Memo HOmolog	452430	453446	1	Protein of unknown function	Protein of unknown function; inhibits haploid invasive growth when overexpressed; synthetically lethal with phospholipase C (PLC1); expression induced by mild heat-stress on a non-fermentable carbon source, upon entry into stationary phase and upon nitrogen deprivation; repressed by inosine and choline in an Opi1p-dependent manner; highly conserved from bacteria to human; Memo, the human homolog, is an ErbB2 interacting protein with an essential function in cell motility
S000003769	YJR009C	TDH2	Triose-phosphate DeHydrogenase	453683	454681	-1	Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), isozyme 2	Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), isozyme 2; involved in glycolysis and gluconeogenesis; tetramer that catalyzes reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in cytoplasm and cell wall; protein abundance increases in response to DNA replication stress; GAPDH-derived antimicrobial peptides are active against a wide variety of wine-related yeasts and bateria; TDH2 has a paralog, TDH3, that arose from the whole genome duplication
S000003770	YJR010C-A	SPC1	Signal Peptidase Complex	458077	458361	-1	Subunit of the signal peptidase complex (SPC)	Subunit of the signal peptidase complex (SPC); SPC cleaves the signal sequence from proteins targeted to the endoplasmic reticulum (ER); homolog of the SPC12 subunit of mammalian signal peptidase complex; protein abundance increases in response to DNA replication stress
S000003771	YJR010W	MET3	METhionine requiring	456239	457774	1	ATP sulfurylase	ATP sulfurylase; catalyzes the primary step of intracellular sulfate activation, essential for assimilatory reduction of sulfate to sulfide, involved in methionine metabolism; human homolog PAPSS2 complements yeast null mutant
S000003772	YJR011C	CAL4	Caf130-Associated regulator of RpL4	458641	459426	-1	Accessory component of the Ccr4-NOT complex	Accessory component of the Ccr4-NOT complex; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS
S000003773	YJR012C	""	""	459795	460193	-1	Protein of unknown function	Protein of unknown function; proposed involvement in transport based on mass spectrometry analysis of copurifying proteins; null mutant is viable
S000003774	YJR013W	GPI14	GlycosylPhosphatidylInositol anchor biosynthesis	460382	461593	1	Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I	Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I; involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M
S000003775	YJR014W	TMA22	Translation Machinery Associated	461829	462425	1	Protein of unknown function	Protein of unknown function; associates with ribosomes and has a putative RNA binding domain; interacts with Tma20p; similar to human GRAP and human DRP1, which interacts with human Tma20p homolog MCT-1; protein abundance increases in response to DNA replication stress
S000003776	YJR015W	""	""	462721	464253	1	Putative protein of unknown function	Putative protein of unknown function; localizes to endoplasmic reticulum and cytoplasm; null mutant exhibits abnormal lipid metabolic phenotype; predicted to encode a membrane transporter based on phylogenetic analysis; not an essential gene; YJR015W has a paralog, SNG1, that arose from the whole genome duplication
S000003777	YJR016C	ILV3	IsoLeucine-plus-Valine requiring	464451	466208	-1	Dihydroxyacid dehydratase	Dihydroxyacid dehydratase; putative 2Fe-2S protein; catalyzes third step in the common pathway leading to biosynthesis of branched-chain amino acids
S000003778	YJR017C	ESS1	ESSential	466521	467033	-1	Peptidylprolyl-cis/trans-isomerase (PPIase)	Peptidylprolyl-cis/trans-isomerase (PPIase); specific for phosphorylated S/T residues N-terminal to proline; regulates phosphorylation of RNAPII large subunit (Rpo21p) C-terminal domain (CTD) at Ser7; associates with phospho-Ser5 form of RNAPII in vivo; present along entire coding length of genes; represses initiation of CUTs; required for efficient termination of mRNA transcription, trimethylation of histone H3; human ortholog PIN1 can complement yeast null and ts mutants
S000003779	YJR018W	""	""	466786	467148	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000003780	YJR019C	TES1	ThioESterase	467232	468281	-1	Peroxisomal acyl-CoA thioesterase	Peroxisomal acyl-CoA thioesterase; likely to be involved in fatty acid oxidation rather than fatty acid synthesis; conserved protein also found in human peroxisomes; TES1 mRNA levels increase during growth on fatty acids
S000003781	YJR020W	""	""	468001	468333	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000003782	YJR021C	REC107	RECombination	468555	469579	-1	Protein involved in early stages of meiotic recombination	Protein involved in early stages of meiotic recombination; involved in coordination between the initiation of recombination and the first division of meiosis; part of a complex (Rec107p-Mei4p-Rec114p) required for ds break formation
S000003783	YJR022W	LSM8	Like SM	469784	470113	1	Lsm (Like Sm) protein	Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) that is part of spliceosomal U6 snRNP and is also implicated in processing of pre-tRNA, pre-snoRNA, and pre-rRNA
S000003785	YJR024C	MDE1	Methylthioribulose-1-phosphate DEhydratase	470230	470964	-1	5'-methylthioribulose-1-phosphate dehydratase	5'-methylthioribulose-1-phosphate dehydratase; acts in the methionine salvage pathway; potential Smt3p sumoylation substrate; expression downregulated by caspofungin and deletion mutant is caspofungin resistant
S000003786	YJR025C	BNA1	Biosynthesis of Nicotinic Acid	471138	471671	-1	3-hydroxyanthranilic acid dioxygenase	3-hydroxyanthranilic acid dioxygenase; required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p
S000003787	YJR026W	""	""	472760	474082	1	Retrotransposon TYA Gag gene co-transcribed with TYB Pol	Retrotransposon TYA Gag gene co-transcribed with TYB Pol; translated as TYA or TYA-TYB polyprotein; Gag is a nucleocapsid protein that is the structural constituent of virus-like particles (VLPs); similar to retroviral Gag
S000003791	YJR030C	RBH2	Ran Binding domain Homolog	483959	486196	-1	Putative protein of unknown function	Putative protein of unknown function; expression repressed in carbon limited vs carbon replete chemostat cultures; non-essential gene; contains a PH-like domain; RBH2 has a paralog, RBH1, that arose from the whole genome duplication
S000003792	YJR031C	GEA1	Guanine nucleotide Exchange on ARF	486586	490812	-1	Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs)	Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA1 has a paralog, GEA2, that arose from the whole genome duplication
S000003793	YJR032W	CPR7	Cyclosporin-sensitive Proline Rotamase	491081	492262	1	Peptidyl-prolyl cis-trans isomerase (cyclophilin)	Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; binds to Hsp82p and contributes to chaperone activity; plays a role in determining prion variants
S000003794	YJR033C	RAV1	Regulator of (H+)-ATPase in Vacuolar membrane	492378	496451	-1	Subunit of RAVE complex (Rav1p, Rav2p, Skp1p)	Subunit of RAVE complex (Rav1p, Rav2p, Skp1p); the RAVE complex promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate
S000003795	YJR034W	PET191	PETite colonies	496683	497009	1	Protein required for assembly of cytochrome c oxidase	Protein required for assembly of cytochrome c oxidase; exists as an oligomer; described as both an integral mitochondrial inner membrane protein facing the intermembrane space (IMS) and as a soluble IMS protein; contains a twin Cx9C motif; imported into the IMS via the MIA import machinery
S000003796	YJR035W	RAD26	RADiation sensitive	497355	500612	1	Protein involved in transcription-coupled nucleotide excision repair	Protein involved in transcription-coupled nucleotide excision repair; repairs UV-induced DNA lesions; recruitment to DNA lesions is dependent on an elongating RNA polymerase II; homolog of human CSB protein
S000003797	YJR036C	HUL4	Hect Ubiquitin Ligase	500713	503391	-1	Protein with similarity to hect domain E3 ubiquitin-protein ligases	Protein with similarity to hect domain E3 ubiquitin-protein ligases; not essential for viability; found in association with Trf4 in TRAMP complex
S000003798	YJR037W	""	""	503102	503485	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified gene HUL4/YJR036C; deletion mutant has decreased spore survival in Drosophila feces
S000003799	YJR038C	""	""	503710	504072	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000003800	YJR039W	MLO127	Mitochondrially LOcalized protein of 127 kDa	503936	507301	1	Mitochondrial protein of unknown function	Mitochondrial protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
S000003801	YJR040W	GEF1	Glycerol Ethanol, Ferric requiring	507746	510085	1	Voltage-gated chloride channel	Voltage-gated chloride channel; localized to the golgi, the endosomal system, and plasma membrane; involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels; modulates TBSV model (+) RNA virus replication by regulating copper metabolism
S000003804	YJR043C	POL32	POLymerase	516461	517513	-1	Third subunit of DNA polymerase delta	Third subunit of DNA polymerase delta; involved in chromosomal DNA replication; required for error-prone DNA synthesis in the presence of DNA damage and processivity; forms a complex with Rev3p, Rev7p and Pol31p; interacts with Hys2p, PCNA (Pol30p), and Pol1p
S000003805	YJR044C	VPS55	Vacuolar Protein Sorting	518763	519185	-1	Late endosomal protein involved in late endosome to vacuole transport	Late endosomal protein involved in late endosome to vacuole transport; functional homolog of human obesity receptor gene-related protein (OB-RGRP)
S000003806	YJR045C	SSC1	Stress-Seventy subfamily C	519638	521602	-1	Hsp70 family ATPase	Hsp70 family ATPase; constituent of the import motor component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); involved in protein translocation and folding; subunit of SceI endonuclease; SSC1 has a paralog, ECM10, that arose from the whole genome duplication
S000003807	YJR046W	TAH11	Topo-A Hypersensitive	522048	523862	1	DNA replication licensing factor	DNA replication licensing factor; required for pre-replication complex assembly
S000003808	YJR047C	ANB1	ANaeroBically induced	524908	525381	-1	Translation elongation factor eIF-5A	Translation elongation factor eIF-5A; previously thought to function in translation initiation; undergoes an essential hypusination modification; expressed under anaerobic conditions; ANB1 has a paralog, HYP2, that arose from the whole genome duplication; human EIF5A complements the inviability of the yeast hyp2 anb1 double null mutant
S000003809	YJR048W	CYC1	CYtochrome C	526335	526664	1	Cytochrome c, isoform 1	Cytochrome c, isoform 1; also known as iso-1-cytochrome c; electron carrier of mitochondrial intermembrane space that transfers electrons from ubiquinone-cytochrome c oxidoreductase to cytochrome c oxidase during cellular respiration; CYC1 has a paralog, CYC7, that arose from the whole genome duplication; human homolog CYC1 can complement yeast null mutant; mutations in human CYC1 cause insulin-responsive hyperglycemia
S000003810	YJR049C	UTR1	Unidentified TRanscript	526884	528476	-1	ATP-NADH kinase	ATP-NADH kinase; phosphorylates both NAD and NADH; active as a hexamer; enhances the activity of ferric reductase (Fre1p); UTR1 has a paralog, YEF1, that arose from the whole genome duplication
S000003811	YJR050W	ISY1	Interactor of SYf1p	528697	529404	1	Member of the NineTeen Complex (NTC)	Member of the NineTeen Complex (NTC); NTC contains Prp19p and stabilizes U6 snRNA in catalytic forms of spliceosome containing U2, U5, and U6 snRNAs; interacts with Prp16p to modulate splicing fidelity; isy1 syf2 cells have defective spindles 
S000003812	YJR051W	OSM1	OSMotic sensitivity	529861	531366	1	Fumarate reductase, catalyzes the reduction of fumarate to succinate	Fumarate reductase, catalyzes the reduction of fumarate to succinate; required for the reoxidation of intracellular NADH under anaerobic conditions; acts as electron acceptor in mitochondrial intermembrane space; has two translation start sites, one at the annotated start codon which produces an ER-targeted form required for anaerobic growth, and one at codon 32 which produces a mitochondrially-targeted form; OSM1 has a paralog, FRD1, that arose from the whole genome duplication
S000003813	YJR052W	RAD7	RADiation sensitive	532062	533759	1	Nucleotide excision repair (NER) protein	Nucleotide excision repair (NER) protein; binds damaged DNA during NER; binds DNA in an ATP-dependent manner (with Rad16p) during NER; required for repair of non-transcribed chromatin; subunit of Nucleotide Excision Repair Factor 4 (NEF4) and the Elongin-Cullin-Socs (ECS) ligase complex
S000003814	YJR053W	BFA1	Byr-Four-Alike	534027	535751	1	Subunit of a two-component GTPase-activating protein, Bfa1p-Bub2p	Subunit of a two-component GTPase-activating protein, Bfa1p-Bub2p; contributes to GAP activity, inactivating Tem1 by stimulating GTP hydrolysis following damage or misalignment of the mitotic spindle; functions as a guanine-nucleotide exchange inhibitor (GDI) for Tem1p; involved in multiple cell cycle checkpoint pathways that control mitotic exit; required when telomeres are damaged, but not for all types of chromosomal DNA damage; phosphorylated by the Polo-like kinase Cdc5p
S000003815	YJR054W	KCH1	Potassium (K) regulator of CcH1	536056	537549	1	Potassium transporter that mediates K+ influx	Potassium transporter that mediates K+ influx; activates high-affinity Ca2+ influx system (HACS) during mating pheromone response; expression up-regulated in response to alpha factor; localized to sites of polarized growth; member of a fungal-specific gene family; potential Cdc28p substrate; KCH1 has a paralog, PRM6, that arose from the whole genome duplication
S000003816	YJR055W	HIT1	HIgh Temperature growth	538772	539266	1	Protein involved in C/D snoRNP assembly	Protein involved in C/D snoRNP assembly; critical for formation of small nucleolar ribonucleoprotein complexes that are required for rRNA processing and 2'-O-methylation; regulates abundance of Rsa1p; required for growth at high temperature; mutations in human homolog ZNHIT3 cause PEHO syndrome, a ribosomopathy characterized by extreme cerebellar atrophy 
S000003817	YJR056C	""	""	541792	542502	-1	Protein of unknown function	Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress
S000003818	YJR057W	CDC8	Cell Division Cycle	544062	544712	1	Nucleoside monophosphate and nucleoside diphosphate kinase	Nucleoside monophosphate and nucleoside diphosphate kinase; functions in the de novo biosynthesis of pyrimidine deoxyribonucleotides; thymidylate/uridylate kinase that converts nucleoside monophosphates, dTMP and dUMP to nucleoside diphosphates, dTDP and dUDP; nucleoside diphosphate kinase that converts nucleoside diphosphates, dTDP and dUDP to dTTP and dUTP; essential for mitotic and meiotic DNA replication; homologous to S. pombe tmp1; human homolog DTYMK can complement the cdc8 null mutant
S000003819	YJR058C	APS2	clathrin Associated Protein complex Small subunit	544732	545175	-1	Small subunit of the clathrin-associated adaptor complex AP-2	Small subunit of the clathrin-associated adaptor complex AP-2; AP-2 is involved in protein sorting at the plasma membrane; related to the sigma subunit of the mammalian plasma membrane clathrin-associated protein (AP-2) complex
S000003820	YJR059W	PTK2	Putative serine/Threonine protein Kinase	545787	548243	1	Serine/threonine protein kinase	Serine/threonine protein kinase; involved in regulation of ion transport across plasma membrane; carboxyl terminus is essential for glucose-dependent Pma1p activation via phosphorylation of Pma1p-Ser899; enhances spermine uptake; PTK2 has a paralog, PTK1, that arose from the whole genome duplication
S000003821	YJR060W	CBF1	Centromere Binding Factor	548759	549814	1	Basic helix-loop-helix (bHLH) protein	Basic helix-loop-helix (bHLH) protein; forms homodimer to bind E-box consensus sequence CACGTG present at MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins, required for chromosome segregation; protein abundance increases in response to DNA replication stress
S000003822	YJR061W	MNN14	MaNNosyltransferase regulator	550511	553318	1	Protein required for N-glycan mannosylphosphorylation	Protein required for N-glycan mannosylphosphorylation; non-essential gene; transcription repressed by Rm101p; MNN14 has a paralog, MNN4, that arose from the whole genome duplication
S000003823	YJR062C	NTA1	N-Terminal Amidase	553476	554849	-1	Amidase	Amidase; removes the amide group from N-terminal asparagine and glutamine residues to generate proteins with N-terminal aspartate and glutamate residues that are targets of ubiquitin-mediated degradation
S000003824	YJR063W	RPA12	RNA Polymerase A	555195	555572	1	RNA polymerase I subunit A12.2	RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex; physically interacts with transcriptional activator Msn4p, to regulate transcription of AYR1, a gene involved in lipid metabolism
S000003826	YJR065C	ARP3	Actin-Related Protein	557809	559158	-1	Essential component of the Arp2/3 complex	Essential component of the Arp2/3 complex; Arp2/3 is a highly conserved actin nucleation center required for the motility and integrity of actin patches; involved in endocytosis and membrane growth and polarity
S000003827	YJR066W	TOR1	Target Of Rapamycin	559416	566828	1	PIK-related protein kinase and rapamycin target	PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; indole-3-acetic acid, an ATP-competitive TORC1 inhibitor, accumulates upon entry into stationary phase; malonyl-CoA is a direct ATP-competitive inhibitor of TORC1; TOR1 has a paralog, TOR2, that arose from the whole genome duplication
S000003828	YJR067C	YAE1	""	567019	567444	-1	Substrate-specific adaptor protein involved in apo-Rli1p maturation	Substrate-specific adaptor protein involved in apo-Rli1p maturation; subunit of the Yae1-Lto1 complex, recruiting apo-Rli1p to the CIA targeting complex, facilitating the insertion of an Fe/S cluster by the Fe-S biosynthetic machinery; deca-GX3 motif crucial for complex formation; essential for aerobic but not anaerobic growth; homolog of human YAE1D1, which is overexpressed in oral cancers; co-expression of YAE1D1 and LTO1 homolog ORAOV1 restores growth and Rli1p maturation after YAE1 depletion
S000003829	YJR068W	RFC2	Replication Factor C	567643	568704	1	Subunit of heteropentameric Replication factor C (RF-C)	Subunit of heteropentameric Replication factor C (RF-C); RF-C is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
S000003830	YJR069C	HAM1	6-n-HydroxylAMinopurine sensitive	568806	569399	-1	Nucleoside triphosphate pyrophosphohydrolase	Nucleoside triphosphate pyrophosphohydrolase; active against various substrates including ITP, dITP and XTP; mediates exclusion of non canonical purines, pyrimidines from dNTP pools; functions with YJL055W to mediate resistance to 5-FU; specifically reduces the incorporation of 5-FU into RNA without affecting uptake or incorporation of uracil into RNA; protein abundance increases in response to DNA replication stress; yeast HAM1 can complement knockdown of human homolog ITPA
S000003831	YJR070C	LIA1	Ligand of eIF5A	569621	570598	-1	Deoxyhypusine hydroxylase	Deoxyhypusine hydroxylase; HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning; protein abundance increases in response to DNA replication stress
S000003833	YJR072C	NPA3	Nucleolar Preribosomal Associated	570967	572124	-1	Member of the conserved GPN-loop GTPase family	Member of the conserved GPN-loop GTPase family; has a role in transport of RNA polymerase II to the nucleus; exhibits GTP-dependent binding to PolII; has ATPase activity; involved in sister chromatid cohesion; phosphorylated by the Pcl1p-Pho85p kinase complex; human homolog XAB1 interacts with human RNA polymerase II; protein abundance increases in response to DNA replication stress
S000003834	YJR073C	OPI3	OverProducer of Inositol	572315	572935	-1	Methylene-fatty-acyl-phospholipid synthase	Methylene-fatty-acyl-phospholipid synthase; catalyzes the last two steps in phosphatidylcholine biosynthesis; also known as phospholipid methyltransferase
S000003835	YJR074W	MOG1	Multicopy suppressor Of ts Gsp1	573095	573751	1	Conserved nuclear protein that interacts with GTP-Gsp1p	Conserved nuclear protein that interacts with GTP-Gsp1p; stimulates nucleotide release from Gsp1p; involved in nuclear protein import; nucleotide release is inhibited by Yrb1p
S000003836	YJR075W	HOC1	Homologous to OCh1	573981	575171	1	Alpha-1,6-mannosyltransferase	Alpha-1,6-mannosyltransferase; involved in cell wall mannan biosynthesis; subunit of a Golgi-localized complex that also contains Anp1p, Mnn9p, Mnn11p, and Mnn10p; identified as a suppressor of a cell lysis sensitive pkc1-371 allele
S000003837	YJR076C	CDC11	Cell Division Cycle	575354	576601	-1	Component of the septin ring that is required for cytokinesis	Component of the septin ring that is required for cytokinesis; present at the ends of rod-like septin hetero-oligomers; C-terminal extension is important for recruitment of Bni5p to the mother-bud neck, which in turn is required for Myo1p recruitment and cytokinesis; septin rings at the mother-bud neck act as scaffolds for recruiting cell division factors and as barriers to prevent diffusion of specific proteins between mother and daughter cells
S000003838	YJR077C	MIR1	Mitochondrial Import Receptor	577255	578190	-1	Mitochondrial phosphate carrier	Mitochondrial phosphate carrier; imports inorganic phosphate into mitochondria; functionally redundant with Pic2p but more abundant than Pic2p under normal conditions; phosphorylated
S000003839	YJR078W	BNA2	Biosynthesis of Nicotinic Acid	578860	580221	1	Tryptophan 2,3-dioxygenase or indoleamine 2,3-dioxygenase	Tryptophan 2,3-dioxygenase or indoleamine 2,3-dioxygenase; required for de novo biosynthesis of NAD+ from tryptophan via kynurenine; up-regulated by telomere uncapping, and interacts genetically with capping gene CDC13; overexpression reduces lipid droplet accumulation by skewing glycolytic flux towards biosynthesis of shikimate, aromatic amino acids, and NAD+ during aging and extends lifespan; regulated by Hst1p and Aftp 
S000003840	YJR079W	""	""	580205	581239	1	Putative protein of unknown function	Putative protein of unknown function; mutation results in impaired mitochondrial respiration
S000003841	YJR080C	AIM24	Altered Inheritance rate of Mitochondria	580432	581616	-1	Protein with a role in determining mitochondrial architecture	Protein with a role in determining mitochondrial architecture; inner membrane protein that interacts physically and genetically with the MICOS complex and is required for its integrity
S000003842	YJR082C	EAF6	Esa1p-Associated Factor	581914	582255	-1	Subunit of the NuA4 acetyltransferase complex	Subunit of the NuA4 acetyltransferase complex; this complex acetylates histone H4 and NuA3 acetyltransferase complex that acetylates histone H3
S000003843	YJR083C	ACF4	Assembly Complementing Factor	582608	583537	-1	Protein of unknown function	Protein of unknown function; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin cytoskeleton organization; potential Cdc28p substrate
S000003844	YJR084W	""	""	583733	585004	1	Protein that forms a complex with Thp3p	Protein that forms a complex with Thp3p; may have a role in transcription elongation and/or mRNA splicing; identified as a COP9 signalosome component but phenotype and interactions suggest it may not be involved with the signalosome
S000003845	YJR085C	TMH11	TMem14 Homolog of 11 kDa	585120	585437	-1	Mitochondrial protein of unknown function	Mitochondrial protein of unknown function; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress
S000003846	YJR086W	STE18	STErile	586068	586400	1	G protein gamma subunit	G protein gamma subunit; forms dimer with Ste4p to activate mating signaling pathway, heterotrimer with Gpa1p, Ste4p to dampen signaling; C-terminus is palmitoylated and farnesylated, which are required for normal signaling; N-terminal phosphorylation of Ste18p functions synergistically with Fus3p docking site on Ste5p to restrict Ste5p plasma membrane recruitment and signaling; combinatorial phosphorylation at S3 and S7, by pheromone and pH stress, modulates Ste18p structure, Fus3p activation
S000003847	YJR087W	""	""	586400	586750	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; partially overlaps the verified genes STE18 and ECM2
S000003848	YJR088C	EMC2	ER Membrane protein Complex	586495	587373	-1	Member of conserved ER transmembrane complex	Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm Y57G7A.10/EMC-2, fly CG17556, human TTC35
S000003850	YJR090C	GRR1	Glucose Repression-Resistant	590872	594327	-1	F-box protein component of an SCF ubiquitin-ligase complex	F-box protein component of an SCF ubiquitin-ligase complex; modular substrate specificity factor which associates with core SCF (Cdc53p, Skp1p and Hrt1p/Rbx1p) to form the SCF(Grr1) complex; SCF(Grr1) acts as a ubiquitin-protein ligase directing ubiquitination of substrates such as: Gic2p, Mks1p, Mth1p, Cln1p, Cln2p and Cln3p; involved in carbon catabolite repression, glucose-dependent divalent cation transport, glucose transport, morphogenesis, and sulfite detoxification
S000003851	YJR091C	JSN1	Just Say No	595061	598336	-1	Member of the Puf family of RNA-binding proteins	Member of the Puf family of RNA-binding proteins; interacts with mRNAs encoding membrane-associated proteins; involved in localizing the Arp2/3 complex to mitochondria; overexpression causes increased sensitivity to benomyl; JSN1 has a paralog, PUF2, that arose from the whole genome duplication
S000003852	YJR092W	BUD4	BUD site selection	598735	603078	1	Anillin-like protein involved in bud-site selection	Anillin-like protein involved in bud-site selection; required for the axial budding pattern; required for the formation and disassembly of the double septin ring structure, and generally for septin organization; functions as a platform linking the cytokinesis tag septins to the axial landmark through its multiple domains; in vivo substrate of Cdc28p/Clb2p
S000003853	YJR093C	FIP1	Factor Interacting with Poly(A) polymerase	603220	604203	-1	Subunit of cleavage polyadenylation factor (CPF)	Subunit of cleavage polyadenylation factor (CPF); interacts directly with poly(A) polymerase (Pap1p) to regulate its activity; bridging factor that links Pap1p and the CPF complex via Yth1p
S000003854	YJR094C	IME1	Inducer of MEiosis	604569	605651	-1	Master regulator of meiosis that is active only during meiotic events	Master regulator of meiosis that is active only during meiotic events; activates transcription of early meiotic genes through interaction with Ume6p; regulator of meiotic commitment; degraded by the 26S proteasome following phosphorylation by Ime2p; transcription is negatively regulated in cis by the IRT1 long noncoding antisense RNA
S000003855	YJR094W-A	RPL43B	Ribosomal Protein of the Large subunit	608305	608858	1	Ribosomal 60S subunit protein L43B	Ribosomal 60S subunit protein L43B; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43B has a paralog, RPL43A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
S000003856	YJR095W	SFC1	Succinate-Fumarate Carrier	609773	610741	1	Mitochondrial succinate-fumarate transporter	Mitochondrial succinate-fumarate transporter; transports succinate into and fumarate out of the mitochondrion; required for ethanol and acetate utilization
S000003857	YJR096W	""	""	611195	612043	1	Xylose and arabinose reductase	Xylose and arabinose reductase; member of the aldo-keto reductase (AKR) family; GFP-fusion protein is induced in response to the DNA-damaging agent MMS
S000003858	YJR097W	JJJ3	J-protein (Type III)	612413	612931	1	Iron-binding protein	Iron-binding protein; involved in targeting cytoplasmic SRP-independent proteins to the ER; contains a CSL Zn finger and a DnaJ-domain; involved in diphthamide biosynthesis; ortholog human Dph4
S000003859	YJR098C	""	""	613186	615156	-1	Putative protein of unknown function	Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
S000003860	YJR099W	YUH1	Yeast Ubiquitin Hydrolase	615576	616286	1	Ubiquitin C-terminal hydrolase	Ubiquitin C-terminal hydrolase; cleaves ubiquitin-protein fusions to generate monomeric ubiquitin; hydrolyzes the peptide bond at the C-terminus of ubiquitin; also the major processing enzyme for the ubiquitin-like protein Rub1p
S000003861	YJR100C	AIM25	Altered Inheritance of Mitochondria	616351	617334	-1	Mitochondria protein of unknown function	Mitochondria protein of unknown function; interacts genetically with TOR1 to regulate chronological lifespan, and the response to both heat shock and oxidative stress; involved in maintaining the integrity of the mitochondrial network; negative regulator of mitophagy flux; non-tagged protein is detected in purified mitochondria in high-throughput studies; null mutant is viable and displays an elevated frequency of mitochondrial genome loss; similar to murine NOR1
S000003862	YJR101W	RSM26	Ribosomal Small subunit of Mitochondria	618234	619034	1	Mitochondrial ribosomal protein of the small subunit	Mitochondrial ribosomal protein of the small subunit
S000003863	YJR102C	VPS25	Vacuolar Protein Sorting	619157	619765	-1	Component of the ESCRT-II complex	Component of the ESCRT-II complex; ESCRT-II is involved in ubiquitin-dependent sorting of proteins into the endosome
S000003864	YJR103W	URA8	URAcil requiring	620754	622490	1	Minor CTP synthase isozyme (see also URA7)	Minor CTP synthase isozyme (see also URA7); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA8 has a paralog, URA7, that arose from the whole genome duplication
S000003865	YJR104C	SOD1	SuperOxide Dismutase	622550	623014	-1	Cytosolic copper-zinc superoxide dismutase	Cytosolic copper-zinc superoxide dismutase; also sulfide oxidase; detoxifies superoxide and hydrogen sulfide; stabilizes Yck1p and Yck2p kinases in glucose to repress respiration; phosphorylated by Dun1p, enters nucleus under oxidative stress to promote transcription of stress response genes; abundance increases under DNA replication stress; localization to mitochondrial intermembrane space is modulated by MICOS complex; human ortholog SOD1 implicated in ALS complements a null allele
S000003866	YJR105W	ADO1	ADenOsine kinase	623581	624603	1	Adenosine kinase	Adenosine kinase; required for the utilization of S-adenosylmethionine (AdoMet); may be involved in recycling adenosine produced through the methyl cycle
S000003867	YJR106W	ECM27	ExtraCellular Mutant	624838	627015	1	Protein involved in calcium homeostasis and exit from quiescence	Protein involved in calcium homeostasis and exit from quiescence; required for proper trehalose levels during quiescence; may play a role in cell wall biosynthesis, mutants are hypersensitive to antifungal, Papulacandin B; null mutants have increased plasmid loss; interacts with Pdr5p
S000003868	YJR107W	LIH1	LIpase Homolog	627340	628326	1	Putative lipase	Putative lipase
S000003869	YJR108W	ABM1	Aberrant Microtubules	628712	629083	1	Protein of unknown function	Protein of unknown function; required for normal microtubule organization
S000003870	YJR109C	CPA2	Carbamyl Phosphate synthetase A	629585	632941	-1	Large subunit of carbamoyl phosphate synthetase	Large subunit of carbamoyl phosphate synthetase; carbamoyl phosphate synthetase catalyzes a step in the synthesis of citrulline, an arginine precursor
S000003871	YJR110W	YMR1	Yeast Myotubularin Related	633615	635681	1	Phosphatidylinositol 3-phosphate (PI3P) phosphatase	Phosphatidylinositol 3-phosphate (PI3P) phosphatase; involved in various protein sorting pathways, including CVT targeting and endosome to vacuole transport; has similarity to the conserved myotubularin dual specificity phosphatase family
S000003872	YJR111C	PXP2	PeroXisomal Protein	635855	636706	-1	Peroxisomal matrix protein with naturally active promoter	Peroxisomal matrix protein with naturally active promoter; well-conserved in fungi; localized to peroxisomes under physiological growth conditions; levels of some amino acids are altered upon both knockout and overexpression, suggesting potential involvement of Pxp2p in amino acid metabolism or related cellular metabolic processes (needs further study); GFP-fusion protein displays inherent dual localization with large proportion localizing to cytosol
S000003873	YJR112W	NNF1	Necessary for Nuclear Function	637030	637635	1	Essential component of the outer kinetochore MIND complex	Essential component of the outer kinetochore MIND complex; joins kinetochore subunits contacting DNA to those contacting microtubules; required for kinetochore bi-orientation and accurate chromosome segregation; complex consists of Mtw1p, Nnf1p, Nsl1p and Dsn1p; homologous to metazoan CENP-H proteins
S000003874	YJR113C	RSM7	Ribosomal Small subunit of Mitochondria	638232	638975	-1	Mitochondrial ribosomal protein of the small subunit	Mitochondrial ribosomal protein of the small subunit; has similarity to E. coli S7 ribosomal protein
S000003875	YJR114W	""	""	638659	639051	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RSM7/YJR113C
S000003876	YJR115W	""	""	639942	640451	1	Putative protein of unknown function	Putative protein of unknown function; YJR115W has a paralog, ECM13, that arose from the whole genome duplication
S000003877	YJR116W	TDA4	Topoisomerase I Damage Affected	640825	641664	1	Protein distantly similar to ceramide synthases, Lag1p and Lac1p	Protein distantly similar to ceramide synthases, Lag1p and Lac1p; null mutant is sensitive to expression of the top1-T722A allele; mutation confers sensitivity to copper
S000003878	YJR117W	STE24	STErile	642007	643368	1	Highly conserved zinc metalloprotease	Highly conserved zinc metalloprotease; component of the ER quality control mechanism that removes faulty proteins clogging translocation channels; inhibits SRP-independent translocation into the ER; has two roles in a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; cleaves isoprenylated and non-prenylated oligopeptides; human homolog ZMPSTE24 implicated in mandibuloacral dysplasia (MAD), and complements the null mutant
S000003879	YJR118C	ILM1	Increased Loss of Mitochondrial DNA	643490	644101	-1	Protein of unknown function	Protein of unknown function; may be involved in mitochondrial DNA maintenance; required for slowed DNA synthesis-induced filamentous growth
S000003880	YJR119C	JHD2	JmjC domain-containing Histone Demethylase	644304	646490	-1	JmjC domain family histone demethylase	JmjC domain family histone demethylase; promotes global demethylation of H3K4 and represses noncoding intergenic transcription during sporulation; removes methyl groups added by Set1p; phosphorylation by Tpk2p during glycolysis inhibits activity, nuclear localization, chromatin binding and promotes Not4p Ub-mediated degradation; negatively regulated by H3K14 acetylation; regulates sporulation timing by extending period of active transcription; regulates rDNA silencing; human homolog is JARID1C
S000003881	YJR120W	DMO1	Determines Mitochondrial prOteome	647126	647476	1	Protein of unknown function	Protein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p
S000003882	YJR121W	ATP2	ATP synthase	647607	649142	1	Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthase	Beta subunit of the F1 sector of mitochondrial F1F0 ATP synthase; which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; F1 translationally regulates ATP6 and ATP8 expression to achieve a balanced output of ATP synthase genes encoded in nucleus and mitochondria; phosphorylated
S000003883	YJR122W	IBA57	Iron-sulfur cluster assembly factor for Biotin synthase and Aconitase-like mitochondrial proteins	649776	651269	1	Protein involved in incorporating iron-sulfur clusters into proteins	Protein involved in incorporating iron-sulfur clusters into proteins; mitochondrial matrix protein; involved in the incorporation of iron-sulfur clusters into mitochondrial aconitase-type proteins; activates the radical-SAM family members Bio2p and Lip5p; interacts with Ccr4p in the two-hybrid system
S000003885	YJR124C	""	""	652892	654238	-1	Putative protein of unknown function	Putative protein of unknown function; expression induced under calcium shortage
S000003886	YJR125C	ENT3	Epsin N-Terminal homology	654737	655963	-1	Protein containing an N-terminal epsin-like domain	Protein containing an N-terminal epsin-like domain; involved in clathrin recruitment and traffic between the Golgi and endosomes; associates with the clathrin adaptor Gga2p
S000003887	YJR126C	VPS70	Vacuolar Protein Sorting	656254	658689	-1	Protein of unknown function involved in vacuolar protein sorting	Protein of unknown function involved in vacuolar protein sorting; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum
S000003888	YJR127C	RSF2	ReSpiration Factor	658917	663059	-1	Zinc-finger protein	Zinc-finger protein; involved in transcriptional control of both nuclear and mitochondrial genes, many of which specify products required for glycerol-based growth, respiration, and other functions; RSF2 has a paralog, TDA9, that arose from the whole genome duplication; relocalizes from nucleus to cytoplasm upon DNA replication stress
S000003889	YJR128W	""	""	662921	663280	1	Putative protein of unknown function	Putative protein of unknown function; conserved among S. cerevisiae strains; partially overlaps the verified ORF RSF2
S000003890	YJR129C	EFM3	Elongation Factor Methyltransferase	664000	665019	-1	S-adenosylmethionine-dependent methyltransferase	S-adenosylmethionine-dependent methyltransferase; seven-beta-strand lysine methyltransferase which trimethylates translation elongation factor EF2 (Eft1p and Eft2p) at lysine 509; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; ortholog of human gene FAM86A
S000003891	YJR130C	STR2	Sulfur TRansfer	665218	667137	-1	Cystathionine gamma-synthase, converts cysteine into cystathionine	Cystathionine gamma-synthase, converts cysteine into cystathionine; STR2 has a paralog, YML082W, that arose from the whole genome duplication
S000003892	YJR131W	MNS1	""	667644	669293	1	Alpha-1,2-mannosidase	Alpha-1,2-mannosidase; involved in ER-associated protein degradation (ERAD); catalyzes the removal of one mannose residue from a glycosylated protein, converting the modification from Man9GlcNAc to Man8GlcNAc; catalyzes the last step in glycoprotein maturation in the ER and is critical for ER protein degradation
S000003894	YJR133W	XPT1	Xanthine Phosphoribosyl Transferase	672991	673620	1	Xanthine-guanine phosphoribosyl transferase	Xanthine-guanine phosphoribosyl transferase; required for xanthine utilization and for optimal utilization of guanine
S000003895	YJR134C	SGM1	Slow growth on Galactose and Mannose	673729	675852	-1	Protein of unknown function	Protein of unknown function; required for wild-type growth rate on galactose and mannose; localizes to COPI coated vesicles and the Golgi apparatus
S000003896	YJR135C	MCM22	MiniChromosome Maintenance	676059	676778	-1	Outer kinetochore protein and component of the Ctf3 subcomplex	Outer kinetochore protein and component of the Ctf3 subcomplex; binds to centromeric DNA in a Ctf19p-dependent manner; involved in chromosome segregation and minichromosome maintenance; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-K and fission yeast sim4
S000003898	YJR137C	MET5	METhionine requiring	678957	683285	-1	Sulfite reductase beta subunit	Sulfite reductase beta subunit; involved in amino acid biosynthesis, transcription repressed by methionine
S000003900	YJR139C	HOM6	HOMoserine requiring	689445	690524	-1	Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase)	Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase); dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions
S000003901	YJR140C	HIR3	HIstone Regulation	690750	695696	-1	Subunit of the HIR complex	Subunit of the HIR complex; a nucleosome assembly complex involved in regulation of histone gene transcription; involved in position-dependent gene silencing and nucleosome reassembly; ortholog of human CABIN1 protein
S000003902	YJR141W	IPA1	Important for cleavage and PolyAdenylation	695906	696949	1	Protein implicated in pre-mRNA processing and proteasomal degradation	Protein implicated in pre-mRNA processing and proteasomal degradation; interacts physically and genetically with mRNA cleavage and polyadenylation factors; mutant is impaired in pre-mRNA cleavage and polyadenylation; associates with several Ub-conjugating enzymes and alters Ub-mediated proteasome activity; localizes to both the nucleus and cytosol; contains a HECT_2 domain; homologous to human ubiquitin-protein ligase, UBE3D, a gene implicated in age-related macular degeneration
S000003903	YJR142W	""	""	697141	698169	1	8-oxo-dGTP diphosphatase of the Nudix hydrolase family	8-oxo-dGTP diphosphatase of the Nudix hydrolase family; converts diphosphates of damaged forms of thiamin to monophosphates; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition
S000003904	YJR143C	PMT4	Protein O-MannosylTransferase	698326	700614	-1	Protein O-mannosyltransferase	Protein O-mannosyltransferase; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; appears to form homodimers in vivo and does not complex with other Pmt proteins; target for new antifungals
S000003905	YJR144W	MGM101	Mitochondrial Genome Maintenance	700882	701691	1	Protein with a role in mitochondrial DNA recombinational repair	Protein with a role in mitochondrial DNA recombinational repair; also involved in interstrand cross-link repair; binds to and catalyzes the annealing of single-stranded mtDNA; oligomerizes to form rings and filaments; related to Rad52-type recombination proteins, with limited overall similarity but sharing conserved functionally important residues; component of the mitochondrial nucleoid, required for the repair of oxidative mtDNA damage and mitochondrial genome maintenance
S000003906	YJR145C	RPS4A	Ribosomal Protein of the Small subunit	702027	703068	-1	Protein component of the small (40S) ribosomal subunit	Protein component of the small (40S) ribosomal subunit; mutation affects 20S pre-rRNA processing; homologous to mammalian ribosomal protein S4, no bacterial homolog; RPS4A has a paralog, RPS4B, that arose from the whole genome duplication
S000003907	YJR146W	""	""	703885	704238	1	Protein of unknown function	Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps HMS2
S000003908	YJR147W	HMS2	High-copy Mep Suppressor	704196	705272	1	Protein with similarity to heat shock transcription factors	Protein with similarity to heat shock transcription factors; overexpression suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant; HMS2 has a paralog, SKN7, that arose from the whole genome duplication
S000003909	YJR148W	BAT2	Branched-chain Amino acid Transaminase	705744	706874	1	Cytosolic branched-chain amino acid (BCAA) aminotransferase	Cytosolic branched-chain amino acid (BCAA) aminotransferase; preferentially involved in BCAA catabolism; homolog of murine ECA39; highly expressed during stationary phase and repressed during logarithmic phase; BAT2 has a paralog, BAT1, that arose from the whole genome duplication
S000003910	YJR149W	""	""	707160	708374	1	Putative protein of unknown function	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
S000003911	YJR150C	DAN1	Delayed ANaerobic	708811	709707	-1	Cell wall mannoprotein	Cell wall mannoprotein; has similarity to Tir1p, Tir2p, Tir3p, and Tir4p; expressed under anaerobic conditions, completely repressed during aerobic growth
S000003912	YJR151C	DAN4	Delayed ANaerobic	712255	715740	-1	Cell wall mannoprotein	Cell wall mannoprotein; has similarity to Tir1p, Tir2p, Tir3p, and Tir4p; expressed under anaerobic conditions, completely repressed during aerobic growth
S000003913	YJR152W	DAL5	Degradation of Allantoin	719666	721297	1	Allantoate permease	Allantoate permease; ureidosuccinate permease; also transports dipeptides, though with lower affinity than for allantoate and ureidosuccinate; expression is constitutive but sensitive to nitrogen catabolite repression
S000003914	YJR153W	PGU1	PolyGalactUronase	722815	723900	1	Endo-polygalacturonase	Endo-polygalacturonase; pectolytic enzyme that hydrolyzes the alpha-1,4-glycosidic bonds in the rhamnogalacturonan chains in pectins
S000003915	YJR154W	""	""	725784	726824	1	Putative protein of unknown function	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
S000003924	YLL001W	DNM1	DyNaMin-related	147890	150163	1	Dynamin-related GTPase involved in mitochondrial organization	Dynamin-related GTPase involved in mitochondrial organization; required for mitochondrial fission and inheritance; self assembles on the cytoplasmic face of mitochondrial tubules at sites where division will occur; participates in endocytosis and regulates peroxisome fission along with Vps1p; mutants in the human ortholog DNM1L, which mediates mitochondrial fission, peroxisomal division, autophagy, and mitophagy, are associated with slowly progressive infantile encephalopathy
S000003925	YLL002W	RTT109	Regulator of Ty1 Transposition	146291	147601	1	Histone acetyltransferase	Histone acetyltransferase; critical for cell survival in presence of DNA damage during S phase, required for recovery after DSB repair; acetylates H3K56, H3K9; H3K56 acetylation activity required for expression homeostasis, buffering of mRNA synthesis rate against changes in gene dosage during S phase; involved in non-homologous end joining and regulation of Ty1 transposition; prevents hyper-amplification of rDNA; interacts physically with Vps75p
S000003926	YLL003W	SFI1	Suppressor of Fermentation Induced loss of stress resistance	143201	146041	1	Centrin (Cdc31p)-binding protein required for SPB duplication	Centrin (Cdc31p)-binding protein required for SPB duplication; localizes to the half-bridge of the spindle pole body (SPB); required for progression through G(2)-M transition; phosphorylated by Cdc28p-Clb2p and by Cdc5p; dephosphorylated by Cdc14p; has similarity to Xenopus laevis XCAP-C
S000003927	YLL004W	ORC3	Origin Recognition Complex	141073	142923	1	Subunit of the origin recognition complex (ORC)	Subunit of the origin recognition complex (ORC); ORC directs DNA replication by binding to replication origins and is also involved in transcriptional silencing
S000003928	YLL005C	SPO75	SPOrulation	137940	140546	-1	Meiosis-specific protein of unknown function	Meiosis-specific protein of unknown function; required for spore wall formation during sporulation; dispensable for both nuclear divisions during meiosis
S000003929	YLL006W	MMM1	Maintenance of Mitochondrial Morphology	136586	137866	1	ER integral membrane protein, ERMES complex subunit	ER integral membrane protein, ERMES complex subunit; ERMES links the ER to mitochondria and may promote inter-organellar calcium and phospholipid exchange as well as coordinating mitochondrial DNA replication and growth; required for mitophagy; ERMES complex is often co-localized with peroxisomes and with concentrated areas of pyruvate dehydrogenase; localizes to the peroxisome in glucose
S000003930	YLL007C	LMO1	eLMO homolog	134302	136299	-1	Homolog of mammalian ELMO (Engulfment and celL MOtility)	Homolog of mammalian ELMO (Engulfment and celL MOtility); upstream component for regulation through the small GTPase Rho5p; may form a complex with Dck1p that acts as a GEF for Rho5p; cytoplasmic protein that relocates to mitochondria under oxidative stress; implicated in mitophagy; not an essential protein
S000003931	YLL008W	DRS1	Deficiency of Ribosomal Subunits	131729	133987	1	Nucleolar DEAD-box protein required for ribosome assembly and function	Nucleolar DEAD-box protein required for ribosome assembly and function; including synthesis of 60S ribosomal subunits; constituent of 66S pre-ribosomal particles
S000003932	YLL009C	COX17	Cytochrome c OXidase	131205	131414	-1	Copper metallochaperone that transfers copper to Sco1p and Cox11p	Copper metallochaperone that transfers copper to Sco1p and Cox11p; eventual delivery to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs; interacts with the MICOS complex, and interaction is promoted by copper ions; human homolog COX17 partially complements yeast null mutant
S000003933	YLL010C	PSR1	Plasma membrane Sodium Response	129330	130613	-1	Plasma membrane-associated protein phosphatase	Plasma membrane-associated protein phosphatase; involved in the general stress response and the inactivating dephosphorylation of Mep2p; required along with binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through Msn2p dephosphorylation, and for inhibition of TORC1 in response to limiting amino acids; regulates the sodium ion stress response with Psr2p; member of the HAD family of protein phosphatases
S000003934	YLL011W	SOF1	Suppressor Of Fibrillarin	127523	128992	1	Protein required for biogenesis of 40S (small) ribosomal subunit	Protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p; essential gene
S000003935	YLL012W	YEH1	Yeast steryl Ester Hydrolase	125534	127255	1	Steryl ester hydrolase	Steryl ester hydrolase; one of three gene products (Yeh1p, Yeh2p, Tgl1p) responsible for steryl ester hydrolase activity and involved in sterol homeostasis; localized to lipid particle membranes; YEH1 has a paralog, YEH2, that arose from the whole genome duplication
S000003936	YLL013C	PUF3	PUmilio-homology domain Family	122075	124714	-1	Protein of the mitochondrial outer surface	Protein of the mitochondrial outer surface; links the Arp2/3 complex with the mitochondria during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins; translationally represses oxidative stress-linked genes; regulates CoQ biosynthesis
S000003937	YLL014W	EMC6	ER Membrane protein Complex	121322	121648	1	Member of conserved ER transmembrane complex	Member of conserved ER transmembrane complex; required for efficient folding of proteins in the ER; null mutant displays induction of the unfolded protein response; homologous to worm F33D4.7/EMC-6, fly CG11781, human TMEM93
S000003938	YLL015W	BPT1	Bile Pigment Transporter	116432	121111	1	ABC type transmembrane transporter of MRP/CFTR family	ABC type transmembrane transporter of MRP/CFTR family; found in vacuolar membrane, involved in the transport of unconjugated bilirubin and in heavy metal detoxification via glutathione conjugates, along with Ycf1p
S000003939	YLL016W	SDC25	Suppressor of cdc25	112847	115993	1	Non-essential Ras guanine nucleotide exchange factor (GEF)	Non-essential Ras guanine nucleotide exchange factor (GEF); localized to the membrane; expressed in poor nutrient conditions and on nonfermentable carbon sources; contains a stop codon in S288C, full-length gene includes YLL017W; SDC25 has a paralog, CDC25, that arose from the whole genome duplication
S000003940	YLL017W	""	""	112234	112545	1	Non-essential Ras guanine nucleotide exchange factor (GEF)	Non-essential Ras guanine nucleotide exchange factor (GEF); localized to the membrane; expressed in poor nutrient conditions and on nonfermentable carbon sources; contains a stop codon in S288C, full-length gene includes YLL016W; SDC25 has a paralog, CDC25, that arose from the whole genome duplication
S000003942	YLL019C	KNS1	Kinase Next to SPA2	105686	107899	-1	Protein kinase involved in negative regulation of PolIII transcription	Protein kinase involved in negative regulation of PolIII transcription; effector kinase of the TOR signaling pathway and phosphorylates Rpc53p to regulate ribosome and tRNA biosynthesis; member of the LAMMER family of protein kinases, which are serine/threonine kinases also capable of phosphorylating tyrosine residues; capable of autophosphorylation
S000003943	YLL020C	""	""	105556	105861	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene KNS1
S000003944	YLL021W	SPA2	Spindle Pole Antigen	100947	105347	1	Component of the polarisome	Component of the polarisome; functions in actin cytoskeletal organization during polarized growth; acts as a scaffold for Mkk1p and Mpk1p cell wall integrity signaling components; potential Cdc28p substrate; coding sequence contains length polymorphisms in different strains; SPA2 has a paralog, SPH1, that arose from the whole genome duplication
S000003946	YLL023C	POM33	POre Membrane, 33 kDa	97997	98836	-1	Transmembrane nucleoporin	Transmembrane nucleoporin; involved in nuclear pore complex (NPC) distribution, assembly or stabilization; highly conserved across species, orthologous to human TMEM33 and paralogous to Per33p; protein abundance increases in response to DNA replication stress
S000003947	YLL024C	SSA2	Stress-Seventy subfamily A	95566	97485	-1	HSP70 family ATP-binding protein	HSP70 family ATP-binding protein; involved in protein folding, vacuolar import of proteins; required for ubiquitin-dependent degradation of short-lived proteins; associated with chaperonin-containing T-complex; 98% identical to paralog Ssa1p with distinct functional specificity in propagation of yeast [URE3] prions and vacuolar-mediated degradation of gluconeogenesis enzymes; binds tRNA, has role in tRNA nuclear import during starvation; targeted to vacuole via AP-3 pathway
S000003948	YLL025W	PAU17	seriPAUperin	94747	95121	1	Protein of unknown function	Protein of unknown function; member of the seripauperin multigene family encoded mainly in subtelomeric regions; SWAT-GFP and mCherry fusion proteins localize to the vacuole; YLL025W is not an essential gene
S000003949	YLL026W	HSP104	Heat Shock Protein	88623	91349	1	Disaggregase	Disaggregase; heat shock protein that cooperates with Ydj1p (Hsp40) and Ssa1p (Hsp70) to refold and reactivate previously denatured, aggregated proteins; responds to stresses including heat, ethanol, and sodium arsenite; involved in [PSI+] propagation; protein becomes more abundant and forms cytoplasmic foci in response to DNA replication stress; potentiated Hsp104p variants decrease TDP-43 proteotoxicity by eliminating its cytoplasmic aggregation
S000003950	YLL027W	ISA1	Iron Sulfur Assembly	87403	88155	1	Protein required for maturation of mitochondrial [4Fe-4S] proteins	Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa2p and possibly Iba57p; isa1 deletion causes loss of mitochondrial DNA and respiratory deficiency; depletion reduces growth on nonfermentable carbon sources; functional ortholog of bacterial A-type ISC proteins; human ISCA1 can complement isa1 null mutant
S000003951	YLL028W	TPO1	Transporter of POlyamines	84804	86564	1	Polyamine transporter of the major facilitator superfamily	Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; catalyzes uptake of polyamines at alkaline pH and excretion at acidic pH; during oxidative stress exports spermine, spermidine from the cell, which controls timing of expression of stress-responsive genes; phosphorylation enhances activity and sorting to the plasma membrane
S000003952	YLL029W	FRA1	Fe Repressor of Activation	81461	83710	1	Protein involved in negative regulation of iron regulon transcription	Protein involved in negative regulation of iron regulon transcription; forms an iron independent complex with Fra2p, Grx3p, and Grx4p; cytosolic; mutant fails to repress transcription of iron regulon and is defective in spore formation
S000003953	YLL030C	RRT7	Regulator of rDNA Transcription	80356	80697	-1	Putative protein of unknown function	Putative protein of unknown function; conserved across S. cerevisiae strains; identified in a screen for mutants with increased levels of rDNA transcription
S000003954	YLL031C	GPI13	GlycosylPhosphatidylInositol anchor biosynthesis	77152	80205	-1	ER membrane localized phosphoryltransferase	ER membrane localized phosphoryltransferase; adds phosphoethanolamine onto the third mannose residue of the glycosylphosphatidylinositol (GPI) anchor precursor; similar to human PIG-O protein
S000003955	YLL032C	""	""	74270	76747	-1	Protein of unknown function	Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene
S000003956	YLL033W	IRC19	Increased Recombination Centers	73409	74101	1	Protein of unknown function	Protein of unknown function; YLL033W is not an essential gene but mutant is defective in spore formation; null mutant displays increased levels of spontaneous Rad52p foci
S000003957	YLL034C	RIX7	RIbosome eXport	70633	73146	-1	Putative ATPase of the AAA family	Putative ATPase of the AAA family; required for export of pre-ribosomal large subunits from the nucleus; distributed between the nucleolus, nucleoplasm, and nuclear periphery depending on growth conditions
S000003958	YLL035W	GRC3	""	68580	70478	1	Polynucleotide kinase present on rDNA	Polynucleotide kinase present on rDNA; required for efficient transcription termination by RNA polymerase I; functions with Las1p in a conserved mechanism to cleave pre-rRNA at a specific site during rRNA processing and ribosome biogenesis; required for cell growth; mRNA is cell-cycle regulated
S000003960	YLL037W	""	""	66562	66942	1	Dubious open reading frame unlikely to encode a functional protein	Dubious open reading frame unlikely to encode a functional protein; overlaps 3' end of essential PRP19 gene encoding an RNA splicing factor
S000003961	YLL038C	ENT4	Epsin N-Terminal homology	65775	66518	-1	Protein of unknown function	Protein of unknown function; contains an N-terminal epsin-like domain; proposed to be involved in the trafficking of Arn1p in the absence of ferrichrome
S000003962	YLL039C	UBI4	Ubiquitin	64062	65207	-1	Ubiquitin	Ubiquitin; becomes conjugated to proteins, marking them for selective degradation via ubiquitin-26S proteasome system; essential for cellular stress response; encoded as polyubiquitin precursor comprised of 5 head-to-tail repeats; number of repeats is evolutionarily unstable within and between yeast species; protein abundance increases in response to DNA replication stress; implicated as modulator of replicative lifespan and meiotic recombination
S000003963	YLL040C	VPS13	Vacuolar Protein Sorting	54211	63645	-1	Lipid transport protein	Lipid transport protein; involved in vacuolar protein sorting, protein retention in the Golgi, prospore membrane formation and sporulation; required for mitochondrial integrity and ER packaging into autophagosomes during cortical reticulophagy; peripheral membrane protein found at the prospore membrane and at membrane contact sites; contains a PH-like domain; homologous to human CHAC and COH1, involved in Chorea-acanthocytosis and Cohen syndrome, respectively
S000003964	YLL041C	SDH2	Succinate DeHydrogenase	53131	53931	-1	Iron-sulfur protein subunit of succinate dehydrogenase	Iron-sulfur protein subunit of succinate dehydrogenase; the complex couples the oxidation of succinate to the transfer of electrons to ubiquinone as part of the TCA cycle and the mitochondrial respiratory chain; other members are Sdh1p, Sdh3p, and Sdh4p
S000003965	YLL042C	ATG10	AuTophaGy related	52087	52590	-1	Conserved E2-like conjugating enzyme	Conserved E2-like conjugating enzyme; mediates formation of the Atg12p-Atg5p conjugate, which is a critical step in autophagy
S000003966	YLL043W	FPS1	FdP1 Suppressor	49938	51947	1	Aquaglyceroporin, plasma membrane channel	Aquaglyceroporin, plasma membrane channel; involved in efflux of glycerol and xylitol, and in transport of acetate, arsenite, and antimonite; key factor in maintaining redox balance by mediating passive diffusion of glycerol; phosphorylated by Hog1p and Slt2p MAPK; deletion improves xylose fermentation; regulated by Rgc1p and Ask10p, which are regulated by Hog1p phosphorylation under osmotic stress; phosphorylation by Ypk1p required to maintain an open state
S000003967	YLL044W	""	""	48216	48662	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; transcription of both YLL044W and the overlapping gene RPL8B is reduced in the gcr1 null mutant
S000003968	YLL045C	RPL8B	Ribosomal Protein of the Large subunit	47859	48629	-1	Ribosomal 60S subunit protein L8A	Ribosomal 60S subunit protein L8A; required for processing of 27SA3 pre-rRNA to 27SB during assembly of large subunit; binds Domain I of 25S, 5.8 S rRNAs; mutation results in decreased amounts of free 60S subunits; RPL8B has paralog RPL8A from whole genome duplication; RPL8A or RPL8B ribosomes not functionally interchangeable; proportions of Rpl8ap, Rpl8bp in 80S ribosome differ in glucose and glycerol; homologous to mammalian ribosomal protein L7A, no bacterial homolog
S000003969	YLL046C	RNP1	RiboNucleoProtein	46714	47463	-1	Ribonucleoprotein that contains two RNA recognition motifs (RRM)	Ribonucleoprotein that contains two RNA recognition motifs (RRM); RNP1 has a paralog, SBP1, that arose from the whole genome duplication
S000003970	YLL047W	""	""	46672	47055	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified gene RNP1
S000003971	YLL048C	YBT1	Yeast Bile Transporter	41280	46265	-1	Transporter of ATP-binding cassette (ABC) family	Transporter of ATP-binding cassette (ABC) family; targeted to vacuole via AP-3 pathway; involved in bile acid transport; negative regulator of vacuole fusion; regulates release of lumenal Ca2+ stores; similar to mammalian bile transporters; YBT1 has a paralog, VMR1, that arose from the whole genome duplication
S000003972	YLL049W	LDB18	Low Dye Binding	40666	41205	1	Component of the dynactin complex	Component of the dynactin complex; dynactin is required for dynein activity; null mutant exhibits defects in nuclear migration and spindle orientation and has reduced affinity for alcian blue dye; has homology to mammalian dynactin subunit p24
S000003973	YLL050C	COF1	COFilin	39804	40414	-1	Cofilin, involved in pH-dependent actin filament depolarization	Cofilin, involved in pH-dependent actin filament depolarization; binds both actin monomers and filaments and severs filaments; involved in the selective sorting, export of the secretory cargo from the late golgi; genetically interacts with pmr1; thought to be regulated by phosphorylation at SER4; ubiquitous and essential in eukaryotes
S000003974	YLL051C	FRE6	Ferric REductase	37333	39471	-1	Putative ferric reductase with similarity to Fre2p	Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels; targeted to vacuole via AP-3 pathway
S000003975	YLL052C	AQY2	AQuaporin from Yeast	35912	36361	-1	Water channel that mediates water transport across cell membranes	Water channel that mediates water transport across cell membranes; only expressed in proliferating cells; controlled by osmotic signals; may be involved in freeze tolerance; disrupted by a stop codon in many S. cerevisiae strains
S000003976	YLL053C	""	""	35503	35961	-1	Putative protein	Putative protein; in the Sigma 1278B strain background YLL053C is contiguous with AQY2 which encodes an aquaporin
S000003977	YLL054C	""	""	32673	35204	-1	Putative protein of unknown function with similarity to Pip2p	Putative protein of unknown function with similarity to Pip2p; an oleate-specific transcriptional activator of peroxisome proliferation; YLL054C is not an essential gene
S000003978	YLL055W	YCT1	Yeast Cysteine Transporter	30109	31704	1	High-affinity cysteine-specific transporter	High-affinity cysteine-specific transporter; has similarity to the Dal5p family of transporters; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YCT1 is not an essential gene
S000003979	YLL056C	""	""	27409	28305	-1	NADH-dependent aldehyde reductase	NADH-dependent aldehyde reductase; reduces many aldehyde compounds including glycolaldehyde, formaldehyde and furfural; transcription unregulated by furfural, 5-hydroxymethylfurfural (HMF) and the mycotoxin patulin; expression increased by multidrug resistance transcription factors Yrm1p and Yrr1p; member of atypical subgroup 7 of the short-chain dehydrogenase/reductase (SDR) family; localizes to the cytoplasm; SWAT-GFP and mCherry fusion proteins localize to the ER and vacuole respectively
S000003980	YLL057C	JLP1	dnaJ-Like Protein	25756	26994	-1	Fe(II)-dependent sulfonate/alpha-ketoglutarate dioxygenase	Fe(II)-dependent sulfonate/alpha-ketoglutarate dioxygenase; involved in sulfonate catabolism for use as a sulfur source; contains sequence that resembles a J domain (typified by the E. coli DnaJ protein); induced by sulphur starvation
S000003981	YLL058W	HSU1	Hydrogen Sulfide Utilizing	23569	25296	1	Inefficient homocysteine synthase	Inefficient homocysteine synthase; catalyzes de novo homocysteine biosynthesis; capable of catalyzing same reaction as Met17p; affects S-methylmethionine metabolism; similar to Str2p, which is a cystathionine gamma-synthase important in sulfur metabolism; HSU1 is not an essential gene
S000003982	YLL059C	""	""	22474	22980	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000003983	YLL060C	GTT2	GlutaThione Transferase	21138	21839	-1	Glutathione S-transferase capable of homodimerization	Glutathione S-transferase capable of homodimerization; functional overlap with Gtt2p, Grx1p, and Grx2p; protein abundance increases in response to DNA replication stress
S000003984	YLL061W	MMP1	S-MethylMethionine Permease	17956	19707	1	High-affinity S-methylmethionine permease	High-affinity S-methylmethionine permease; required for utilization of S-methylmethionine as a sulfur source; has similarity to S-adenosylmethionine permease Sam3p
S000003985	YLL062C	MHT1	S-Methylmethionine-Homocysteine methylTransferase	16639	17613	-1	S-methylmethionine-homocysteine methyltransferase	S-methylmethionine-homocysteine methyltransferase; functions along with Sam4p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio
S000003986	YLL063C	AYT1	AcetYlTransferase	14648	16072	-1	Acetyltransferase	Acetyltransferase; catalyzes trichothecene 3-O-acetylation, suggesting a possible role in trichothecene biosynthesis; localized to peroxisomes
S000003991	YLR001C	""	""	151389	153977	-1	Putative protein of unknown function	Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; predicted to be palmitoylated
S000003992	YLR002C	NOC3	NucleOlar Complex associated	154343	156334	-1	Subunit of a nuclear complex with Noc2p and pre-replicative complexes	Subunit of a nuclear complex with Noc2p and pre-replicative complexes; the Noc2p-Noc3p complex binds to 66S ribosomal precursors to mediate their maturation and intranuclear transport; binds to chromatin at active replication origins, and is required for pre-RC formation and maintenance during DNA replication licensing
S000003993	YLR003C	CMS1	Complementation of Mcm-10 Suppressor	156855	157730	-1	Putative subunit of the 90S preribosome processome complex	Putative subunit of the 90S preribosome processome complex; overexpression rescues supressor mutant of mcm10; null mutant is viable; relocalizes from nucleus to cytoplasm upon DNA replication stress
S000003994	YLR004C	THI73	THIamine regulon	157934	159505	-1	Putative plasma membrane permease	Putative plasma membrane permease; proposed to be involved in carboxylic acid uptake and repressed by thiamine; substrate of Dbf2p/Mob1p kinase; transcription is altered if mitochondrial dysfunction occurs
S000003995	YLR005W	SSL1	Suppressor of Stem-Loop mutation	160049	161434	1	Subunit of the core form of RNA polymerase transcription factor TFIIH	Subunit of the core form of RNA polymerase transcription factor TFIIH; has both protein kinase and DNA-dependent ATPase/helicase activities; essential for transcription and nucleotide excision repair; interacts with Tfb4p
S000003996	YLR006C	SSK1	Suppressor of Sensor Kinase	161755	163893	-1	Cytoplasmic phosphorelay intermediate osmosensor and regulator	Cytoplasmic phosphorelay intermediate osmosensor and regulator; part of a two-component signal transducer that mediates osmosensing via a phosphorelay mechanism; required for mitophagy; dephosphorylated form is degraded by the ubiquitin-proteasome system; potential Cdc28p substrate
S000003997	YLR007W	NSE1	Non-SMC Element	164392	165402	1	Component of the SMC5-SMC6 complex	Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair
S000003998	YLR008C	PAM18	Presequence translocase-Associated Motor	165577	166083	-1	Subunit of the import motor (PAM complex)	Subunit of the import motor (PAM complex); the PAM complex is a  component of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); essential J-protein cochaperone that stimulates Ssc1p ATPase activity to drive import; inhibited by Pam16p
S000003999	YLR009W	RLP24	Ribosomal-Like Protein	166537	167136	1	Essential protein required for ribosomal large subunit biogenesis	Essential protein required for ribosomal large subunit biogenesis; associated with pre-60S ribosomal subunits; stimulates the ATPase activity of Afg2p, which is required for release of Rlp24p from the pre-60S particle; has similarity to Rpl24Ap and Rpl24Bp
S000004000	YLR010C	TEN1	TElomeric pathways with STn1	167320	167802	-1	Protein that regulates telomeric length	Protein that regulates telomeric length; protects telomeric ends in a complex with Cdc13p and Stn1p; similar to human Ten1 which is critical for the telomeric function of the CST (Cdc13p-Stn1p-Ten1p) complex
S000004001	YLR011W	LOT6	LOw Temperature-responsive	169103	169678	1	FMN-dependent NAD(P)H:quinone reductase	FMN-dependent NAD(P)H:quinone reductase; role in apoptosis-like cell death; may be involved in quinone detoxification; expression elevated at low temperature; sequesters the Cin5p transcription factor in the cytoplasm in complex with the proteasome under reducing conditions
S000004002	YLR012C	""	""	169982	170281	-1	Putative protein of unknown function	Putative protein of unknown function; YLR012C is not an essential gene
S000004003	YLR013W	GAT3	""	171339	171764	1	Protein containing GATA family zinc finger motifs	Protein containing GATA family zinc finger motifs; involved in spore wall assembly; sequence similarity to GAT4, and the double mutant gat3 gat4 exhibits reduced dityrosine fluorescence relative to the single mutants
S000004004	YLR014C	PPR1	Pyrimidine Pathway Regulation	172268	174982	-1	Zinc finger transcription factor	Zinc finger transcription factor; contains a Zn(2)-Cys(6) binuclear cluster domain, positively regulates transcription of URA1, URA3, URA4, and URA10, which are involved in de novo pyrimidine biosynthesis, in response to pyrimidine starvation; activity may be modulated by interaction with Tup1p
S000004005	YLR015W	BRE2	BREfeldin A sensitivity	175227	176744	1	Subunit of COMPASS (Set1C) complex	Subunit of COMPASS (Set1C) complex; COMPASS methylates Lys4 of histone H3 and functions in silencing at telomeres; has a C-terminal Sdc1 Dpy-30 Interaction (SDI) domain that mediates binding to Sdc1p; similar to trithorax-group protein ASH2L
S000004006	YLR016C	PML1	Pre-mRNA Leakage	176802	177416	-1	Subunit of the RES complex	Subunit of the RES complex; RES complex is required for nuclear retention of unspliced pre-mRNAs; acts in the same pathway as Pml39p and Mlp1p
S000004007	YLR017W	MEU1	Multicopy Enhancer of UAS2	177608	178621	1	Methylthioadenosine phosphorylase (MTAP)	Methylthioadenosine phosphorylase (MTAP); catalyzes the initial step in the methionine salvage pathway; affects polyamine biosynthesis through regulation of ornithine decarboxylase (Spe1p) activity; regulates ADH2 gene expression
S000004008	YLR018C	POM34	POre Membrane	178707	179606	-1	Subunit of the transmembrane ring of the nuclear pore complex (NPC)	Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication
S000004009	YLR019W	PSR2	Plasma membrane Sodium Response	180288	181481	1	Putative plasma membrane phosphatase	Putative plasma membrane phosphatase; involved in the general stress response and inactivating dephosphorylation of Mep2p; required along with Psr1p and binding partner Whi2p for full activation of STRE-mediated gene expression, possibly through Msn2p dephosphorylation, and for inhibition of TORC1 in response to limiting amino acids; regulates the sodium ion stress response with Psr1p; member of the HAD family of protein phosphatases
S000004010	YLR020C	YEH2	Yeast steryl Ester Hydrolase	181789	183405	-1	Steryl ester hydrolase	Steryl ester hydrolase; catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism; YEH2 has a paralog, YEH1, that arose from the whole genome duplication
S000004011	YLR021W	IRC25	Increased Recombination Centers	183623	184162	1	Component of a heterodimeric Poc4p-Irc25p chaperone	Component of a heterodimeric Poc4p-Irc25p chaperone; involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions; upregulates proteasome assembly in response to the unfolded protein response activated by mistargeting of proteins (UPRam)
S000004012	YLR022C	SDO1	""	184174	184926	-1	Guanine nucleotide exchange factor (GEF) for Ria1p	Guanine nucleotide exchange factor (GEF) for Ria1p; essential protein involved in ribosome maturation; with Ria1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; ortholog of the human protein (SBDS) responsible for autosomal recessive Shwachman-Bodian-Diamond Syndrome; highly conserved across archaea and eukaryotes
S000004013	YLR023C	IZH3	Implicated in Zinc Homeostasis	185498	187129	-1	Membrane protein involved in zinc ion homeostasis	Membrane protein involved in zinc ion homeostasis; member of the four-protein IZH family, expression induced by zinc deficiency; deletion reduces sensitivity to elevated zinc and shortens lag phase, overexpression reduces Zap1p activity
S000004014	YLR024C	UBR2	""	187663	193281	-1	Cytoplasmic ubiquitin-protein ligase (E3)	Cytoplasmic ubiquitin-protein ligase (E3); component of the Mub1p-Ubr2p-Rad6p ubiquitin ligase complex required for the ubiquitination and degradation of Rpn4p; mediates formation of the ternary complex
S000004015	YLR025W	SNF7	Sucrose NonFermenting	194452	195174	1	One of four subunits of the ESCRT-III complex	One of four subunits of the ESCRT-III complex; involved in the sorting of transmembrane proteins into the multivesicular body (MVB) pathway; recruited from the cytoplasm to endosomal membranes; ESCRT-III stands for endosomal sorting complex required for transport III
S000004016	YLR026C	SED5	Suppressor of Erd2 Deletion	195450	196472	-1	cis-Golgi t-SNARE syntaxin	cis-Golgi t-SNARE syntaxin; required for vesicular transport between the ER and the Golgi complex; binds at least 9 SNARE proteins; plays a role in autophagy by regulating formation of autophagic vesicles in Golgi
S000004017	YLR027C	AAT2	Aspartate AminoTransferase	196829	198085	-1	Cytosolic aspartate aminotransferase involved in the metabolism of nit	Cytosolic aspartate aminotransferase involved in the metabolism of nitrogen and amino acids such as aspartate; localizes to and functions in peroxisomes in oleate-grown cells; moonlighting role as a ribosome-associated protein that modulates translation in response to stress
S000004018	YLR028C	ADE16	ADEnine	199540	201315	-1	Enzyme of 'de novo' purine biosynthesis	Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE16 has a paralog, ADE17, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine
S000004020	YLR030W	""	""	203290	204081	1	Putative protein of unknown function	Putative protein of unknown function; S288C contains an in-frame stop codon between ORFs YLR030W and YLR031W
S000004021	YLR031W	""	""	204225	204785	1	Putative protein of unknown function	Putative protein of unknown function; S288C contains an in-frame stop codon between ORFs YLR030W and YLR031W; YLR031W has a paralog, YMR124W, that arose from the whole genome duplication
S000004022	YLR032W	RAD5	RADiation sensitive	204991	208500	1	DNA helicase/Ubiquitin ligase	DNA helicase/Ubiquitin ligase; involved in error-free DNA damage tolerance (DDT), replication fork regression during postreplication repair by template switching, and error-prone translesion synthesis; required for UV and MMS-associated unequal sister chromatid exchange (SCE) but not for double-strand break associated unequal SCE; promotes synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; human homolog HLTF can complement yeast null mutant 
S000004023	YLR033W	RSC58	Remodel the Structure of Chromatin	208761	210269	1	Component of the RSC chromatin remodeling complex	Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
S000004024	YLR034C	SMF3	""	210512	211933	-1	Putative divalent metal ion transporter involved in iron homeostasis	Putative divalent metal ion transporter involved in iron homeostasis; transcriptionally regulated by metal ions; member of Nramp family of metal transport proteins; targeted to vacuole via AP-3 pathway; protein abundance increases in response to DNA replication stress
S000004025	YLR035C	MLH2	MutL Homolog	212369	214456	-1	Protein involved in mismatch repair and meiotic recombination	Protein involved in mismatch repair and meiotic recombination; only certain frameshift intermediates are mismatch repair substrates; forms a complex with Mlh1p, plays accessory role in stimulating activity of MLH1/PMS1 complex
S000004026	YLR036C	""	""	221521	222132	-1	Putative protein predicted to have transmembrane domains	Putative protein predicted to have transmembrane domains; interacts with HSP90 by yeast two-hybrid analysis; YLR036C is not an essential protein
S000004027	YLR037C	PAU23	seriPAUperin family	222685	223059	-1	Cell wall mannoprotein	Cell wall mannoprotein; has similarity to Tir1p, Tir2p, Tir3p, and Tir4p; member of the seripauperin multigene family encoded mainly in subtelomeric regions; expressed under anaerobic conditions, completely repressed during aerobic growth
S000004028	YLR038C	COX12	Cytochrome c OXidase	224921	225172	-1	Subunit VIb of cytochrome c oxidase	Subunit VIb of cytochrome c oxidase; cytochrome c oxidase is also known as respiratory Complex IV and is the terminal member of the mitochondrial inner membrane electron transport chain; required for assembly and activity of cytochrome c oxidase; phosphorylated; easily released from the intermembrane space, suggesting a loose association with Complex IV
S000004029	YLR039C	RIC1	RIbosome Control	225426	228596	-1	Protein involved in retrograde transport to the cis-Golgi network	Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes
S000004030	YLR040C	AFB1	A-Factor Barrier	228908	229582	-1	MATalpha-specific a-factor blocker	MATalpha-specific a-factor blocker; contributes to mating efficiency under certain conditions; localizes to the cell wall; predicted to be a GPI-attached protein; upregulated by Mcm1p-Alpha1p transcription factor; partially overlaps the dubious ORF YLR041W
S000004031	YLR041W	""	""	229377	229697	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YLR040C
S000004032	YLR042C	NFG1	Negative regulator of the Filamentous Growth MAPK pathway	229966	230451	-1	Negative regulator of the filamentous growth pathway	Negative regulator of the filamentous growth pathway; inhibits invasive growth by dampening the activity of the filamentous MAPK pathway; localizes to the cell wall and to the vacuole and cell periphery in HTP studies; deletion improves xylose fermentation in industrially engineered strains; nonessential gene
S000004033	YLR043C	TRX1	ThioRedoXin	231702	232013	-1	Cytoplasmic thioredoxin isoenzyme	Cytoplasmic thioredoxin isoenzyme; part of the thioredoxin system that protects cells from oxidative and reductive stress; subunit of the LMA1 complex, with Pbi2p, that promotes vacuole inheritance; cofactor for Tsa1p; required for ER-Golgi transport; facilitates mitochondrial import of small Tims (Tim9p, Tim10p, Tim13p) by maintaining them in reduced form along with Trx2p; component of a signal transduction redox relay in a light sensing pathway; abundance increases under DNA replication stress
S000004034	YLR044C	PDC1	Pyruvate DeCarboxylase	232390	234081	-1	Major of three pyruvate decarboxylase isozymes	Major of three pyruvate decarboxylase isozymes; key enzyme in alcoholic fermentation; decarboxylates pyruvate to acetaldehyde; involved in amino acid catabolism; subject to glucose-, ethanol-, and autoregulation; activated by phosphorylation in response to glucose levels; N-terminally propionylated in vivo; protein tyrosine nitration on Tyr157 or Tyr344 inhibits activity and impairs fermentation
S000004036	YLR046C	""	""	238001	238813	-1	Putative membrane protein	Putative membrane protein; member of the fungal lipid-translocating exporter (LTE) family of proteins; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; YLR046C has a paralog, RTA1, that arose from the whole genome duplication
S000004037	YLR047C	FRE8	""	239347	241407	-1	Protein with sequence similarity to iron/copper reductases	Protein with sequence similarity to iron/copper reductases; involved in iron homeostasis; deletion mutant has iron deficiency/accumulation growth defects; expression increased in the absence of copper-responsive transcription factor Mac1p
S000004038	YLR048W	RPS0B	Ribosomal Protein of the Small subunit	242232	243349	1	Protein component of the small (40S) ribosomal subunit	Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2
S000004039	YLR049C	MLO50	Mitochondrially LOcalized protein of 50 kDa	243886	245172	-1	Mitochondrial protein of unknown function	Mitochondrial protein of unknown function
S000004040	YLR050C	EMA19	Efficient Mitochondria targeting-Associated protein	245587	246072	-1	Integral ER membrane protein involved in protein sorting	Integral ER membrane protein involved in protein sorting; component of ER-SURF, a pathway involved in retrieving mitochondrial membrane protein precursors and rerouting them to the mitochondrial translocation system; promotes the proteolytic degradation of non-productive mitochondrial precursor proteins suggesting involvement in quality control during protein import; GFP-fusion protein localizes to the ER; member of a conserved family of ER proteins including human homolog TMEM97
S000004042	YLR052W	IES3	Ino Eighty Subunit	247201	247953	1	Subunit of the INO80 chromatin remodeling complex	Subunit of the INO80 chromatin remodeling complex
S000004043	YLR053C	NRS1	Nitrogen-Responsive Start regulator	248100	248426	-1	Nitrogen-responsive activator of Start	Nitrogen-responsive activator of Start; nuclear microprotein with transcriptional activator activity; bypasses Start arrest in a cln3 bck2 double mutant when overexpressed; interacts with subunits of the SBF complex (Swi4p-Swi6p); induced by nitrogen limitation and rapamycin with peak abundance at Start; proposed to promote the G1/S transition by rewiring the G1/S transcriptional machinery under poor nitrogen conditions upstream of the Whi5-inhibited form of SBF and independent of Cln3 and Bck2
S000004044	YLR054C	OSW2	Outer Spore Wall	248717	250978	-1	Protein of unknown function reputedly involved in spore wall assembly	Protein of unknown function reputedly involved in spore wall assembly
S000004045	YLR055C	SPT8	SuPpressor of Ty	251272	253080	-1	Subunit of the SAGA transcriptional regulatory complex	Subunit of the SAGA transcriptional regulatory complex; not present in SAGA-like complex SLIK/SALSA; required for SAGA-mediated inhibition at some promoters
S000004046	YLR056W	ERG3	ERGosterol biosynthesis	253861	254958	1	C-5 sterol desaturase	C-5 sterol desaturase; glycoprotein that catalyzes the introduction of a C-5(6) double bond into episterol, a precursor in ergosterol biosynthesis; transcriptionally down-regulated when ergosterol is in excess; mutants are viable, but cannot grow on non-fermentable carbon sources; substrate of HRD ubiquitin ligase; mutation is functionally complemented by human SC5D
S000004047	YLR057W	MNL2	MaNnosidase-Like protein	255306	257855	1	Putative mannosidase involved in ER-associated protein degradation	Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene
S000004048	YLR058C	SHM2	Serine HydroxyMethyltransferase	257992	259401	-1	Cytosolic serine hydroxymethyltransferase	Cytosolic serine hydroxymethyltransferase; converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis
S000004049	YLR059C	REX2	Rna EXonuclease	259738	260547	-1	3'-5' RNA exonuclease	3'-5' RNA exonuclease; involved in 3'-end processing of U4 and U5 snRNAs, 5S and 5.8S rRNAs, and RNase P and RNase MRP RNA; localized to mitochondria and null suppresses escape of mtDNA to nucleus in yme1 yme2 mutants; RNase D exonuclease; role in preventing L-A mycovirus pathogenesis
S000004051	YLR061W	RPL22A	Ribosomal Protein of the Large subunit	263194	263948	1	Ribosomal 60S subunit protein L22A	Ribosomal 60S subunit protein L22A; required for translation of long 5' UTR of IME1 mRNA and meiotic entry; required for the oxidative stress response, pseudohyphal and invasive growth; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication
S000004052	YLR062C	BUD28	BUD site selection	263577	263954	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene RPL22A; diploid mutant displays a weak budding pattern phenotype in a systematic assay
S000004053	YLR063W	BMT6	Base Methyltransferase of Twenty five S rRNA	264157	265254	1	Methyltransferase required for m3U2843 methylation of the 25S rRNA	Methyltransferase required for m3U2843 methylation of the 25S rRNA; S-adenosylmethionine-dependent; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR063W is not an essential gene
S000004054	YLR064W	PER33	Pore and ER protein, 33 kDa	265456	266277	1	Protein that localizes to the endoplasmic reticulum	Protein that localizes to the endoplasmic reticulum; also associates with the nuclear pore complex; deletion extends chronological lifespan; highly conserved across species, orthologous to human TMEM33 and paralogous to Pom33p; protein abundance increases in response to DNA replication stress
S000004055	YLR065C	SND2	SRP-iNDependent targeting	266373	266918	-1	Protein involved in SRP-independent targeting of substrates to the ER	Protein involved in SRP-independent targeting of substrates to the ER; component of an alternative ER targeting pathway that has partial functional redundancy with the GET pathway; preference for substrates with downstream transmembrane domains; interacts with Snd1p, Pho88p/Snd3p and Sec61p-translocon subunits; can compensate for loss of SRP; role in the late endosome-vacuole interface; putative role in secretory protein quality control
S000004056	YLR066W	SPC3	Signal Peptidase Complex	267169	267723	1	Subunit of signal peptidase complex	Subunit of signal peptidase complex; complex catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; homologous to mammalian SPC22/23; other members of the complex are Spc1p, Spc2p, and Sec11p
S000004057	YLR067C	PET309	PETite colonies	267813	270710	-1	Specific translational activator for the COX1 mRNA	Specific translational activator for the COX1 mRNA; binds to the COX1 mRNA; also influences stability of intron-containing COX1 primary transcripts; localizes to the mitochondrial inner membrane; contains 12 pentatricopeptide repeats (PPRs)
S000004058	YLR068W	FYV7	Function required for Yeast Viability	271008	271463	1	Nucleolar protein required for maturation of 18S rRNA	Nucleolar protein required for maturation of 18S rRNA; required for survival upon exposure to K1 killer toxin
S000004059	YLR069C	MEF1	Mitochondrial Elongation Factor	271630	273915	-1	Mitochondrial elongation factor involved in translational elongation	Mitochondrial elongation factor involved in translational elongation
S000004060	YLR070C	XYL2	""	274141	275211	-1	Xylitol dehydrogenase	Xylitol dehydrogenase; converts xylitol to D-xylulose; expression induced by xylose, even though this pentose sugar is not well utilized by S. cerevisiae; null mutant has cell wall defect
S000004061	YLR071C	RGR1	Resistant to Glucose Repression	275378	278626	-1	Subunit of the RNA polymerase II mediator complex	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for glucose repression, HO repression, RME1 repression and sporulation
S000004062	YLR072W	LAM6	Lipid transfer protein Anchored at Membrane contact site	278862	280943	1	Sterol transporter that transfers sterols between membranes	Sterol transporter that transfers sterols between membranes; may regulate and coordinate formation of contact sites between organelles; localizes to ER-mitochondrial contact sites in a Tom70p- and Tom71p-dependent manner; mitochondrial localization requires GRAM domain; also localizes to ER-vacuole contact sites, in a Vac8p-dependent manner; has GRAM and StART-like (VASt) domains; one of six StART-like domain-containing proteins in yeast; conserved across eukaryotes
S000004063	YLR073C	RFU1	Regulator of Free Ubiquitin chains	281019	281621	-1	Protein that inhibits Doa4p deubiquitinating activity	Protein that inhibits Doa4p deubiquitinating activity; contributes to ubiquitin homeostasis by regulating the conversion of free ubiquitin chains to ubiquitin monomers by Doa4p; GFP-fusion protein localizes to endosomes
S000004064	YLR074C	BUD20	BUD site selection	281956	282456	-1	C2H2-type zinc finger protein required for ribosome assembly	C2H2-type zinc finger protein required for ribosome assembly; shuttling factor which associates with pre-60S particles in the nucleus, accompanying them to the cytoplasm; cytoplasmic dissociation of Bud20p requires Drg1p; N-terminus harbors a nuclear localization signal (NLS) and a nuclear export signal (NES); cytoplasmic Bud20p is reimported by Kap123-dependent pathway; involved in bud-site selection; diploid mutants display a random budding pattern; similar to human ZNF593
S000004066	YLR076C	""	""	283185	283607	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPL10 which encodes the ribosomal protein L10
S000004067	YLR077W	FMP25	Found in Mitochondrial Proteome	283872	285623	1	Protein required for assembly of respiratory complex III	Protein required for assembly of respiratory complex III; mitochondrial inner membrane protein; required for an early step in assembly of respiratory complex III (cytochrome bc1 complex); mRNA is targeted to mitochondria
S000004068	YLR078C	BOS1	Bet One Suppressor	285736	286559	-1	v-SNARE (vesicle specific SNAP receptor)	v-SNARE (vesicle specific SNAP receptor); localized to the endoplasmic reticulum membrane and necessary for vesicular transport from the ER to the Golgi; required for efficient nuclear fusion during mating
S000004069	YLR079W	SIC1	Substrate/Subunit Inhibitor of Cyclin-dependent protein kinase	286820	287674	1	Cyclin-dependent kinase inhibitor (CKI)	Cyclin-dependent kinase inhibitor (CKI); inhibitor of Cdc28-Clb kinase complexes that controls G1/S phase transition, preventing premature S phase and ensuring genomic integrity; phosphorylated by Clb5/6-Cdk1 and Cln1/2-Cdk1 kinase which regulate timing of Sic1p degradation; phosphorylation targets Sic1p for SCF(CDC4)-dependent turnover; functional homolog of mammalian Kip1
S000004070	YLR080W	EMP46	""	287916	289250	1	Integral membrane component of ER-derived COPII-coated vesicles	Integral membrane component of ER-derived COPII-coated vesicles; functions in ER to Golgi transport; EMP46 has a paralog, EMP47, that arose from the whole genome duplication
S000004071	YLR081W	GAL2	GALactose metabolism	290212	291936	1	Galactose permease	Galactose permease; required for utilization of galactose; also able to transport glucose
S000004072	YLR082C	SRL2	Suppressor of Rad53 null Lethality	292394	293572	-1	Protein of unknown function	Protein of unknown function; overexpression suppresses the lethality caused by a rad53 null mutation
S000004073	YLR083C	EMP70	""	294091	296094	-1	Protein with a role in cellular adhesion and filamentous growth	Protein with a role in cellular adhesion and filamentous growth; also endosome-to-vacuole sorting; similar to Tmn3p; member of Transmembrane Nine family of proteins with 9 transmembrane segments; EMP70 has a paralog, TMN2, that arose from the whole genome duplication
S000004074	YLR084C	RAX2	""	296589	300251	-1	Protein required for the maintenance of bud site selection	Protein required for the maintenance of bud site selection; localizes; localization to the bud neck and previous sites of cell division (bud scars; cytokinesis remnants (CRMs)) is interdependent with Rax2p; required with Rax1p to anchor both Nba1p and Nis1p to CRMs where a Cdc42p inhibitory zone is established preventing repolarization of cells at previous division sites; RAX2 mRNA stability is regulated by Mpt5p; predicted type I membrane-spanning protein; N-glycosylated
S000004075	YLR085C	ARP6	Actin-Related Protein	300673	301989	-1	Actin-related protein that binds nucleosomes	Actin-related protein that binds nucleosomes; a component of the SWR1 complex, which exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A
S000004076	YLR086W	SMC4	Structural Maintenance of Chromosomes	302243	306499	1	Subunit of the condensin complex	Subunit of the condensin complex; condensin reorganizes chromosomes during both mitosis and meiosis; forms a subcomplex with Smc2p that has ATP-hydrolyzing and DNA-binding activity, but other condensin subunits are required for chromatin binding; required for tRNA gene clustering at the nucleolus; potential Cdc28p substrate
S000004077	YLR087C	CSF1	Cold Sensitive for Fermentation	306855	315731	-1	Protein with structural similarity to lipid transport protein Vps13p	Protein with structural similarity to lipid transport protein Vps13p; localizes to multiple membrane contact sites between organelles; role in phospholipid homeostasis and lipidomic adaptation to cold; required for fermentation at low temperature; plays a role in phosphatidylethanolamine metabolism and secretory protein maturation; non-tagged protein is detected in highly purified mitochondria in HTP studies
S000004078	YLR088W	GAA1	GPI Anchor Attachment	316107	317951	1	Subunit of the GPI:protein transamidase complex	Subunit of the GPI:protein transamidase complex; removes the GPI-anchoring signal and attaches GPI (glycosylphosphatidylinositol) to proteins in the ER; human homolog GPAA1 can complement growth defects of yeast thermosensitive mutant at restrictive temperature
S000004079	YLR089C	ALT1	ALanine Transaminase	318237	320015	-1	Alanine transaminase (glutamic pyruvic transaminase)	Alanine transaminase (glutamic pyruvic transaminase); involved in alanine biosynthesis and catabolism; TOR1-independent role in determining chronological lifespan; expression is induced in the presence of alanine; repression is mediated by Nrg1p; ALT1 has a paralog, ALT2, that arose from the whole genome duplication; Alt2p is catalytically inactive
S000004080	YLR090W	XDJ1	""	320701	322080	1	Chaperone with a role in facilitating mitochondrial protein import	Chaperone with a role in facilitating mitochondrial protein import; ascomycete-specific member of the DnaJ-like family, closely related to Ydj1p; predicted to be C-terminally prenylated; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
S000004081	YLR091W	GEP5	GEnetic interactors of Prohibitins	322297	323178	1	Protein of unknown function	Protein of unknown function; required for efficient 5' processing of mitochondrial tRNAs, for respiratory growth and mitochondrial genome maintenance; null mutant has decreased levels of cardiolipin and phosphatidylethanolamine; localizes to the matrix side of the inner mitochondrial membrane
S000004082	YLR092W	SUL2	SULfate	323544	326225	1	High affinity sulfate permease	High affinity sulfate permease; sulfate uptake is mediated by specific sulfate transporters Sul1p and Sul2p, which control the concentration of endogenous activated sulfate intermediates
S000004083	YLR093C	NYV1	New Yeast V-SNARE	326513	327415	-1	v-SNARE component of vacuolar SNARE complex	v-SNARE component of vacuolar SNARE complex; involved in vesicle fusion; inhibits ATP-dependent Ca(2+) transport activity of Pmc1p in vacuolar membrane; targeted to vacuole via AP-3 pathway
S000004084	YLR094C	GIS3	GIg1-2 Suppressor	327730	329238	-1	Protein of unknown function	Protein of unknown function
S000004085	YLR095C	IOC2	Iswi One Complex	329677	332115	-1	Subunit of the Isw1b complex	Subunit of the Isw1b complex; exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing; contains a PHD finger motif; other complex members are Isw1p and Ioc4p
S000004086	YLR096W	KIN2	KINase	332590	336033	1	S/T protein kinase	S/T protein kinase; regulates polarized exocytosis and the Ire1p-mediated UPR; regulates HAC1 mRNA translocation, splicing and translation with KIN1 during ER stress; direct phosphorylation of Sec9p (S190) is enhanced by prior substrate priming (S192); localizes to the cytoplasmic face of the PM and sites of polarized growth; may regulate septin and cell wall organization; activation loop phosphorylation (T281) required for full kinase activity; orthologous to MARK/PAR-1, AMPK family members
S000004087	YLR097C	HRT3	High level expression Reduces Ty3 transposition	336231	337265	-1	Putative SCF-ubiquitin ligase F-box protein	Putative SCF-ubiquitin ligase F-box protein; based on both genetic and physical interactions and sequence similarity; identified in association with Cdc53p, Skp1p and Ubi4 in large and small-scale studies
S000004088	YLR098C	CHA4	Catabolism of Hydroxy Amino acids	337527	339473	-1	DNA binding transcriptional activator	DNA binding transcriptional activator; mediates serine/threonine activation of the catabolic L-serine (L-threonine) deaminase (CHA1); Zinc-finger protein with Zn[2]-Cys[6] fungal-type binuclear cluster domain
S000004089	YLR099C	ICT1	Increased Copper Tolerance	339744	340928	-1	Lysophosphatidic acid acyltransferase	Lysophosphatidic acid acyltransferase; responsible for enhanced phospholipid synthesis during organic solvent stress; null displays increased sensitivity to Calcofluor white; highly expressed during organic solvent stress; ICT1 has a paralog, ECM18, that arose from the whole genome duplication; human ABHD5 can complement ict1 null mutant
S000004092	YLR102C	APC9	Anaphase Promoting Complex	342970	343767	-1	Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C)	Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
S000004093	YLR103C	CDC45	Cell Division Cycle	343989	345941	-1	DNA replication initiation factor	DNA replication initiation factor; recruited to MCM pre-RC complexes at replication origins; promotes release of MCM from Mcm10p, recruits elongation machinery; binds tightly to ssDNA, which disrupts interaction with the MCM helicase and stalls it during replication stress; mutants in human homolog may cause velocardiofacial and DiGeorge syndromes
S000004094	YLR104W	LCL2	Long Chronological Lifespan	346585	346980	1	Putative protein of unknown function	Putative protein of unknown function; mutant is deficient in cell wall mannosylphosphate and has long chronological lifespan; genetic interactions suggest a role in ER-associated protein degradation (ERAD); SWAT-GFP fusion protein localizes to the endoplasmic reticulum and vacuole, while mCherry fusion localizes to just the vacuole
S000004095	YLR105C	SEN2	Splicing ENdonuclease	347047	348180	-1	Subunit of the tRNA splicing endonuclease	Subunit of the tRNA splicing endonuclease; tRNA splicing endonuclease (Sen complex) is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen complex also cleaves the CBP1 mRNA at the mitochondrial surface; Sen2p contains the active site for tRNA 5' splice site cleavage and has similarity to Sen34p and to Archaeal tRNA splicing endonuclease; localizes to the peroxisome in a Pex5p-dependent manner in oleate
S000004096	YLR106C	REA1	Ribosome Export/Assembly	349006	363738	-1	Huge dynein-related AAA-type ATPase (midasin)	Huge dynein-related AAA-type ATPase (midasin); forms extended pre-60S particle with the Rix1 complex; involved with interaction partners Rsa4p and Ytm1p, in the ATP-dependent remodeling of the pre-60S particle at successive maturation steps during ribosomal biogenesis; involved in the removal of biogenesis factors including GTPase Nog2p prior to nuclear export; contains a hexameric AAA-motor head domain and a long flexible tail with a MIDAS (metal ion-dependent adhesion site) domain
S000004097	YLR107W	REX3	Rna EXonuclease	364116	365330	1	RNA exonuclease	RNA exonuclease; required for maturation of the RNA component of RNase MRP; functions redundantly with Rnh70p and Rex2p in processing of U5 snRNA and RNase P RNA; member of RNase D family of exonucleases
S000004098	YLR108C	""	""	366667	368124	-1	Protein of unknown function	Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YLR108C is not an esssential gene; protein abundance increases in response to DNA replication stress; YLR108C has a paralog, YDR132C, that arose from the whole genome duplication
S000004099	YLR109W	AHP1	Alkyl HydroPeroxide reductase	368781	369311	1	Thiol-specific peroxiredoxin	Thiol-specific peroxiredoxin; reduces hydroperoxides to protect against oxidative damage; function in vivo requires covalent conjugation to Urm1p
S000004100	YLR110C	CCW12	Covalently linked Cell Wall protein	369697	370098	-1	Cell wall mannoprotein	Cell wall mannoprotein; plays a role in maintenance of newly synthesized areas of cell wall; localizes to periphery of small buds, septum region of larger buds, and shmoo tip; CCW12 has a paralog, YDR134C, that arose from the whole genome duplication
S000004101	YLR111W	""	""	370391	370723	1	Putative protein of unknown function	Putative protein of unknown function; conserved across S. cerevisiae strains
S000004102	YLR112W	""	""	370791	371210	1	Putative protein of unknown function	Putative protein of unknown function; conserved across S. cerevisiae strains
S000004103	YLR113W	HOG1	High Osmolarity Glycerol response	371620	372927	1	Mitogen-activated protein kinase involved in osmoregulation	Mitogen-activated protein kinase involved in osmoregulation; controls global reallocation of RNAPII during osmotic shock; mediates recruitment/activation of RNAPII at Hot1p-dependent promoters; binds calmodulin; stimulates antisense transcription to activate CDC28; defines novel S-phase checkpoint with Mrc1p that prevent replication/transcription conflicts; nuclear form represses pseudohyphal growth; autophosphorylates; protein abundance increases under DNA replication stress
S000004104	YLR114C	AVL9	Apl2 Vps1 Lethal	374944	377238	-1	Conserved protein involved in exocytic transport from the Golgi	Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress
S000004105	YLR115W	CFT2	Cleavage Factor Two	377985	380564	1	Subunit of the mRNA cleavage and polyadenlylation factor (CPF)	Subunit of the mRNA cleavage and polyadenlylation factor (CPF); required for pre-mRNA cleavage, polyadenylation and poly(A) site recognition, 43% similarity with the mammalian CPSF-100 protein.
S000004106	YLR116W	MSL5	Mud Synthetic-Lethal	380822	382252	1	Component of commitment complex	Component of commitment complex; which defines first step in splicing pathway; essential protein that interacts with Mud2p and Prp40p, forming a bridge between the intron ends; also involved in nuclear retention of pre-mRNA; relocalizes to the cytosol in response to hypoxia
S000004107	YLR117C	CLF1	Crooked neck-Like Factor	382471	384534	-1	Member of the NineTeen Complex (NTC)	Member of the NineTeen Complex (NTC); this complex contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins
S000004108	YLR118C	TML25	acyl-protein Thioesterase with Multiple Localizations, protein of 25 kDa	384725	385408	-1	Acyl-protein thioesterase responsible for depalmitoylation of Gpa1p	Acyl-protein thioesterase responsible for depalmitoylation of Gpa1p; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus and is induced in response to the DNA-damaging agent MMS
S000004109	YLR119W	SRN2	Suppressor of Rna mutations, Number 2	385534	386175	1	Component of the ESCRT-I complex	Component of the ESCRT-I complex; ESCRT-I is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus
S000004110	YLR120C	YPS1	YaPSin	386511	388220	-1	Aspartic protease	Aspartic protease; hyperglycosylated member of the yapsin family of proteases, attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor; involved in nutrient limitation-induced cleavage of the extracellular inhibitory domain of signaling mucin Msb2p, resulting in activation of the filamentous growth MAPK pathway; involved with other yapsins in the cell wall integrity response; role in KEX2-independent processing of the alpha factor precursor
S000004111	YLR121C	YPS3	YaPSin	388744	390270	-1	Aspartic protease	Aspartic protease; member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor
S000004112	YLR122C	""	""	390954	391331	-1	Putative protein of unknown function	Putative protein of unknown function; conserved among S. cerevisiae strains; partially overlaps dubious ORF YLR123C
S000004113	YLR123C	""	""	391078	391407	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR122C; contains characteristic aminoacyl-tRNA motif
S000004114	YLR124W	""	""	391600	391944	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000004115	YLR125W	""	""	393484	393894	1	Putative protein of unknown function	Putative protein of unknown function; mutant has decreased Ty3 transposition; YLR125W is not an essential gene
S000004116	YLR126C	""	""	394765	395520	-1	Putative glutamine amidotransferase	Putative glutamine amidotransferase; has Aft1p-binding motif in the promoter; may be involved in copper and iron homeostasis; YLR126C is not an essential protein; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
S000004117	YLR127C	APC2	Anaphase Promoting Complex	395758	398319	-1	Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C)	Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the catalytic core of the APC/C; has similarity to cullin Cdc53p
S000004118	YLR128W	DCN1	Defective in Cullin Neddylation	398530	399433	1	Scaffold-type E3 ligase	Scaffold-type E3 ligase; required for cullin neddylation and ubiquitin ligase activation; contains a ubiquitin-binding domain (UBA) for ubiquitin and Nedd8 (Rub1p) interaction and a PONY domain involved in cullin binding and neddylation
S000004119	YLR129W	DIP2	DOM34 Interacting Protein	399657	402488	1	Nucleolar protein	Nucleolar protein; specifically associated with the U3 snoRNA, part of the large ribonucleoprotein complex known as the small subunit (SSU) processome, required for 18S rRNA biogenesis, part of the active pre-rRNA processing complex
S000004120	YLR130C	ZRT2	Zinc-Regulated Transporter	402794	404062	-1	Low-affinity zinc transporter of the plasma membrane	Low-affinity zinc transporter of the plasma membrane; transcription is induced under low-zinc conditions by the Zap1p transcription factor
S000004121	YLR131C	ACE2	Activator of CUP1 Expression	404510	406822	-1	Transcription factor required for septum destruction after cytokinesis	Transcription factor required for septum destruction after cytokinesis; part of the RAM network that regulates polarity and morphogenesis; NES phosphorylation by RAM network kinase Cbk1p blocks nuclear exit in mother cells during the M/G1 transition, causing asymmetric localization to daughter cell nuclei, and increased Ace2p activity; phosphorylation by Cdc28p and Pho85p prevents nuclear import during cell cycle phases other than cytokinesis; Spt16p is required for nuclear exclusion during G1
S000004122	YLR132C	USB1	U Six Biogenesis	407283	408155	-1	Putative poly(U)-specific 3'-to-5' RNA exonuclease	Putative poly(U)-specific 3'-to-5' RNA exonuclease; involved in 3'-end processing of U6 snRNA removing uridines and generating a terminal 2&#8242;,3&#8242; cyclic phosphate; essential protein that localizes to the nucleus and mitochondria; overexpression suppresses the respiratory defects of oxa1 and mtf2 mutants; homolog of S.pombe gene, mpn1 and human gene, hUSB1; mutations in hUSB1 are associated with a rare genodermatosis, poikiloderma with neutropenia (OMIM 604173)
S000004123	YLR133W	CKI1	Choline KInase	408445	410193	1	Choline kinase	Choline kinase; catalyzes the first step in phosphatidylcholine synthesis via the CDP-choline (Kennedy pathway); exhibits some ethanolamine kinase activity contributing to phosphatidylethanolamine synthesis via the CDP-ethanolamine pathway; CKI1 has a paralog, EKI1, that arose from the whole genome duplication
S000004124	YLR134W	PDC5	Pyruvate DeCarboxylase	410723	412414	1	Minor isoform of pyruvate decarboxylase	Minor isoform of pyruvate decarboxylase; key enzyme in alcoholic fermentation, decarboxylates pyruvate to acetaldehyde, regulation is glucose- and ethanol-dependent, repressed by thiamine, involved in amino acid catabolism
S000004125	YLR135W	SLX4	Synthetic Lethal of unknown (X) function	413281	415527	1	Endonuclease involved in processing DNA	Endonuclease involved in processing DNA; acts during recombination, repair; promotes template switching during break-induced replication (BIR), causing non-reciprocal translocations (NRTs); cleaves branched structures in complex with Slx1p; involved in interstrand cross-link repair, Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during DSBR via single-strand annealing; relative distribution to nuclear foci increases upon DNA replication stress; FANCP-related factor
S000004126	YLR136C	TIS11	similar to the mammalian TPA Induced Sequence gene family	415801	416658	-1	mRNA-binding protein expressed during iron starvation	mRNA-binding protein expressed during iron starvation; binds to a sequence element in the 3'-untranslated regions of specific mRNAs to mediate their degradation; involved in iron homeostasis; represses mitochondrial function; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress; TIS11 has a paralog, CTH1, that arose from the whole genome duplication
S000004127	YLR137W	RKM5	Ribosomal lysine (K) Methyltransferase	417006	418109	1	Protein lysine methyltransferase	Protein lysine methyltransferase; monomethylates Lys-46 of the ribosomal large subunit Rpl1a/Rpl1b; member of the seven beta-strand methyltransferase superfamily; orthologs only found among fungal species
S000004128	YLR138W	NHA1	Na+/H+ Antiporter	418437	421394	1	Na+/H+ antiporter	Na+/H+ antiporter; involved in sodium and potassium efflux through the plasma membrane; required for alkali cation tolerance at acidic pH
S000004129	YLR139C	SLS1	Synthetic Lethal with SSM4	421542	423473	-1	Mitochondrial membrane protein	Mitochondrial membrane protein; coordinates expression of mitochondrially-encoded genes; may facilitate delivery of mRNA to membrane-bound translation machinery
S000004130	YLR140W	""	""	423474	423800	1	Dubious open reading frame unlikely to encode a functional protein	Dubious open reading frame unlikely to encode a functional protein; overlaps essential RRN5 gene which encodes a member of the UAF transcription factor involved in transcription of rDNA by RNA polymerase I
S000004131	YLR141W	RRN5	Regulation of RNA polymerase I	423683	424774	1	Protein involved in transcription of rDNA by RNA polymerase I	Protein involved in transcription of rDNA by RNA polymerase I; transcription factor, member of UAF (upstream activation factor) family along with Rrn9p and Rrn10p
S000004132	YLR142W	PUT1	Proline UTilization	425186	426616	1	Proline oxidase	Proline oxidase; nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; PUT1 transcription is induced by Put3p in the presence of proline and the absence of a preferred nitrogen source
S000004133	YLR143W	DPH6	DiPHthamide biosynthesis	427329	429386	1	Diphthamide synthetase	Diphthamide synthetase; catalyzes the last amidation step of diphthamide biosynthesis using ammonium and ATP; evolutionarily conserved in eukaryotes; dph6 mutants exhibit diphthine accumulation and resistance to sordarin, which is indicative of defects in diphthamide formation on EF2; green fluorescent protein (GFP)-tagged protein localizes to the cytoplasm; DPH6/YLR143W is not an essential gene
S000004134	YLR144C	ACF2	Assembly Complementing Factor	429677	432016	-1	Intracellular beta-1,3-endoglucanase	Intracellular beta-1,3-endoglucanase; expression is induced during sporulation; may have a role in cortical actin cytoskeleton assembly; protein abundance increases in response to DNA replication stress
S000004135	YLR145W	RMP1	RNase MRP Protein	432168	432773	1	Subunit of RNase MRP	Subunit of RNase MRP; RNase MRP processes pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; unlike most subunits, not shared between RNase MRP and nuclear RNase P
S000004136	YLR146C	SPE4	SPErmidine auxotroph	432823	433725	-1	Spermine synthase	Spermine synthase; required for the biosynthesis of spermine and also involved in biosynthesis of pantothenic acid
S000004138	YLR148W	PEP3	carboxyPEPtidase Y-deficient	434641	437397	1	Component of CORVET membrane tethering complex	Component of CORVET membrane tethering complex; vacuolar peripheral membrane protein that promotes vesicular docking/fusion reactions in conjunction with SNARE proteins, required for vacuolar biogenesis
S000004139	YLR149C	GID11	Glucose Induced Degradation deficient	437631	439823	-1	Substrate receptor of the glucose-induced degradation-deficient (GID) 	Substrate receptor of the glucose-induced degradation-deficient (GID) complex that targets gluconeogenic enzymes for proteasomal degradation upon switch from ethanol to glucose as a carbon source; overexpression causes a cell cycle delay or arrest; null mutation results in a decrease in plasma membrane electron transport; protein abundance increases in response to DNA replication stress
S000004140	YLR150W	STM1	Suppressor of ToM1	440467	441288	1	Protein required for optimal translation under nutrient stress	Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery
S000004141	YLR151C	PCD1	Peroxisomal Coenzyme A Diphosphatase	441715	442737	-1	8-oxo-dGTP diphosphatase	8-oxo-dGTP diphosphatase; prevents spontaneous mutagenesis via sanitization of oxidized purine nucleoside triphosphates; can also act as peroxisomal pyrophosphatase with specificity for coenzyme A and CoA derivatives, may function to remove potentially toxic oxidized CoA disulfide from peroxisomes to maintain the capacity for beta-oxidation of fatty acids; nudix hydrolase family member; similar E. coli MutT and human, rat and mouse MTH1
S000004142	YLR152C	""	""	442958	444688	-1	Putative protein of unknown function	Putative protein of unknown function; YLR152C is not an essential gene
S000004143	YLR153C	ACS2	Acetyl CoA Synthetase	445524	447575	-1	Acetyl-coA synthetase isoform	Acetyl-coA synthetase isoform; along with Acs1p, acetyl-coA synthetase isoform is the nuclear source of acetyl-coA for histone acetylation; mutants affect global transcription; required for growth on glucose; expressed under anaerobic conditions
S000004144	YLR154C	RNH203	RNase H	447982	448314	-1	Ribonuclease H2 subunit	Ribonuclease H2 subunit; required for RNase H2 activity; role in ribonucleotide excision repair; related to human AGS3 that causes Aicardi-Goutieres syndrome
S000004153	YLR163C	MAS1	Mitochondrial ASsembly	491866	493254	-1	Beta subunit of the mitochondrial processing protease (MPP)	Beta subunit of the mitochondrial processing protease (MPP); essential processing enzyme that cleaves the N-terminal targeting sequences from mitochondrially imported proteins
S000004154	YLR164W	SHH4	SDH4 Homolog	493883	494389	1	Putative alternate subunit of succinate dehydrogenase (SDH)	Putative alternate subunit of succinate dehydrogenase (SDH); mitochondrial inner membrane protein; genetic interaction with SDH4 suggests that Shh4p can function as a functional SDH subunit; a fraction copurifies with SDH subunit Sdh3p; expression induced by nitrogen limitation in a GLN3, GAT1-dependent manner; Shh4p has greater similarity to human SDHD (subunit D of SDH, implicated in paraganglioma) than does its paralog Sdh4p
S000004155	YLR165C	PUS5	PseudoUridine Synthase	494494	495258	-1	Pseudouridine synthase	Pseudouridine synthase; catalyzes only the formation of pseudouridine (Psi)-2819 in mitochondrial 21S rRNA; not essential for viability
S000004156	YLR166C	SEC10	SECretory	495429	498044	-1	Essential 100kDa subunit of the exocyst complex	Essential 100kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion
S000004157	YLR167W	RPS31	Ribosomal Protein of the Small subunit	498947	499405	1	Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin	Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog
S000004158	YLR168C	UPS2	UnProceSsed	499578	500270	-1	Mitochondrial intermembrane space protein	Mitochondrial intermembrane space protein; involved in phospholipid metabolism; forms complex with Mdm35p that transfers phosphatidylserine from outer membrane to inner membrane for phosphatidylethanolamine synthesis; null mutant has defects in mitochondrial morphology; similar to Ups1p, Ups3p and to human PRELI; UPS2 has a paralog, UPS3, that arose from the whole genome duplication
S000004159	YLR169W	""	""	500335	500688	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000004160	YLR170C	APS1	clathrin Associated Protein complex Small subunit	500579	501049	-1	Small subunit of the clathrin-associated adaptor complex AP-1	Small subunit of the clathrin-associated adaptor complex AP-1; AP-1 is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex
S000004161	YLR171W	""	""	500733	501122	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000004162	YLR172C	DPH5	DiPHthamide	501260	502162	-1	Methyltransferase required for synthesis of diphthamide	Methyltransferase required for synthesis of diphthamide; diphthamide is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm
S000004163	YLR173W	TAG1	Termination of AutophaGy	502421	504247	1	Vacuolar membrane protein involved in termination of autophagy	Vacuolar membrane protein involved in termination of autophagy; regulates autophagy termination during persistent nitrogen starvation through Atg1p-mediated re-phosphorylation of Atg13p and PAS disassembly; type II membrane protein containing hydrophobic transmembrane region with N-terminal region oriented toward cytosol and C-terminus inside lumen; luminal domain is glycosylated; forms foci in vacuole membrane during starvation; targeted to vacuole via AP-3 pathway
S000004164	YLR174W	IDP2	Isocitrate Dehydrogenase, NADP-specific	504591	505829	1	Cytosolic NADP-specific isocitrate dehydrogenase	Cytosolic NADP-specific isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate; levels are elevated during growth on non-fermentable carbon sources and reduced during growth on glucose; IDP2 has a paralog, IDP3, that arose from the whole genome duplication; mutation in human homolog IDH1 is associated with low-grade gliomas and secondary glioblastomas
S000004165	YLR175W	CBF5	Centromere Binding Factor	506134	507585	1	Pseudouridine synthase catalytic subunit of box H/ACA snoRNPs	Pseudouridine synthase catalytic subunit of box H/ACA snoRNPs; acts on large and small rRNAs, on snRNA U2, and on some mRNAs; mutations in human ortholog dyskerin cause the disorder dyskeratosis congenita; small nucleolar ribonucleoprotein particles are also known as snoRNPs
S000004166	YLR176C	RFX1	Regulatory Factor X	507797	510232	-1	Major transcriptional repressor of DNA-damage-regulated genes	Major transcriptional repressor of DNA-damage-regulated genes; recruits repressors Tup1p and Cyc8p to their promoters; involved in DNA damage and replication checkpoint pathway; similar to a family of mammalian DNA binding RFX1-4 proteins
S000004167	YLR177W	""	""	511054	512940	1	Putative protein of unknown function	Putative protein of unknown function; phosphorylated by Dbf2p-Mob1p in vitro; some strains contain microsatellite polymophisms at this locus; not an essential gene; YLR177W has a paralog, PSP1, that arose from the whole genome duplication
S000004168	YLR178C	TFS1	cdc Twenty-Five Suppressor	513162	513821	-1	Inhibitor of carboxypeptidase Y (Prc1p), and Ras GAP (Ira2p)	Inhibitor of carboxypeptidase Y (Prc1p), and Ras GAP (Ira2p); phosphatidylethanolamine-binding protein (PEBP) family member and ortholog of hPEBP1/RKIP, a natural metastasis suppressor; targets to vacuolar membranes during stationary phase; acetylated by NatB N-terminal acetyltransferase; protein abundance increases in response to DNA replication stress
S000004169	YLR179C	""	""	514108	514713	-1	Protein of unknown function with similarity to Tfs1p	Protein of unknown function with similarity to Tfs1p; transcription is activated by paralogous proteins Yrm1p and Yrr1p along with proteins involved in multidrug resistance; GFP-tagged protein localizes to the cytoplasm and nucleus
S000004170	YLR180W	SAM1	S-AdenosylMethionine requiring	515262	516410	1	S-adenosylmethionine synthetase	S-adenosylmethionine synthetase; catalyzes transfer of the adenosyl group of ATP to the sulfur atom of methionine; SAM1 has a paralog, SAM2, that arose from the whole genome duplication
S000004171	YLR181C	VTA1	VpsTwenty Associated	516678	517670	-1	Multivesicular body (MVB) protein	Multivesicular body (MVB) protein; involved in endosomal protein sorting; regulates Vps4p activity by promoting its oligomerization; has an N-terminal Vps60- and Did2- binding domain, a linker region, and a C-terminal Vps4p binding domain
S000004172	YLR182W	SWI6	SWItching deficient	517940	520351	1	Transcription cofactor	Transcription cofactor; forms complexes with Swi4p and Mbp1p to regulate transcription at the G1/S transition; involved in meiotic gene expression; also binds Stb1p to regulate transcription at START; cell wall stress induces phosphorylation by Mpk1p, which regulates Swi6p localization; required for the unfolded protein response, independently of its known transcriptional coactivators
S000004173	YLR183C	TOS4	Target Of Sbf	520543	522012	-1	Putative transcription factor, contains Forkhead Associated domain	Putative transcription factor, contains Forkhead Associated domain; binds chromatin; involved in expression homeostasis, buffering of mRNA synthesis rate against gene dosage changes during S phase; target of SBF transcription factor; expression is periodic and peaks in G1; involved in DNA replication checkpoint response; interacts with Rpd3 and Set3 histone deacetylase complexes; APCC(Cdh1) substrate; relative distribution to nucleus increases upon DNA replication stress
S000004174	YLR184W	""	""	522106	522453	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000004175	YLR185W	RPL37A	Ribosomal Protein of the Large subunit	522663	523288	1	Ribosomal 60S subunit protein L37A	Ribosomal 60S subunit protein L37A; required for processing of 27SB pre-rRNA and formation of stable 66S assembly intermediates; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37A has a paralog, RPL37B, that arose from the whole genome duplication
S000004177	YLR187W	SKG3	Suppressor of lethality of Kex2 Gas1 double null mutant	524865	527945	1	Protein of unknown function	Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; potential Cdc28p substrate; similar to Skg4p; relocalizes from bud neck to cytoplasm upon DNA replication stress; SKG3 has a paralog, CAF120, that arose from the whole genome duplication
S000004178	YLR188W	MDL1	MultiDrug resistance-Like	528300	530387	1	Mitochondrial inner membrane half-type ABC transporter	Mitochondrial inner membrane half-type ABC transporter; mediates export of peptides generated upon proteolysis of mitochondrial proteins; plays a role in the regulation of cellular resistance to oxidative stress
S000004179	YLR189C	ATG26	AuTophaGy related	530797	534393	-1	UDP-glucose:sterol glucosyltransferase	UDP-glucose:sterol glucosyltransferase; conserved enzyme involved in synthesis of sterol glucoside membrane lipids; in contrast to ATG26 from P. pastoris, S. cerevisiae ATG26 is not involved in autophagy
S000004180	YLR190W	MMR1	Mitochondrial Myo2p Receptor-related	535214	536689	1	Phospholipid binding protein	Phospholipid binding protein; interacts with mitochondria and with Myo2p, functioning as an adaptor that recruits Myo2p and facilitates actin-based transport of mitochondria to the bud; mediates mitochondria anchorage at the bud tip; phosphorylated protein that localizes to the mitochondrial outer membrane; mRNA is targeted to the bud via the transport system involving She2p; member of the DSL1 family of tethering proteins
S000004181	YLR191W	PEX13	PEroXin	537272	538432	1	Peroxisomal importomer complex component	Peroxisomal importomer complex component; integral peroxisomal membrane protein required for docking and translocation of peroxisomal matrix proteins; interacts with the PTS1 signal recognition factor Pex5p and the PTS2 signal recognition factor Pex7p; forms a complex with Pex14p and Pex17p; human homolog PEX13 complements yeast null mutant
S000004182	YLR192C	HCR1	High-Copy suppressor of Rpg1	538792	539589	-1	eIF3j component of translation initiation factor 3 (eIF3)	eIF3j component of translation initiation factor 3 (eIF3); dual function protein involved in translation initiation as a substoichiometric component (eIF3j) of eIF3; required for 20S pre-rRNA processing; required at post-transcriptional step for efficient retrotransposition; absence decreases Ty1 Gag:GFP protein levels; binds eIF3 subunits Rpg1p, Prt1p and 18S rRNA; eIF3 also involved in programmed stop codon read through; human homolog EIF3J can complement yeast hcr1 mutant
S000004183	YLR193C	UPS1	UnProceSsed	540009	540536	-1	Phosphatidic acid transfer protein	Phosphatidic acid transfer protein; plays a role in phospholipid metabolism by transporting phosphatidic acid from the outer to the inner mitochondrial membrane; localizes to the mitochondrial intermembrane space; null mutant has altered cardiolipin and phosphatidic acid levels; ortholog of human PRELI
S000004184	YLR194C	NCW2	Novel Cell Wall protein	540809	541573	-1	GPI-anchored protein involved in cell wall remodeling	GPI-anchored protein involved in cell wall remodeling; involved in the assembly of the cell wall chitin-glucan structure and repair of damage caused by the antifungal agent polyhexamethylene biguanide; role in the cell wall integrity pathway; expression is upregulated in response to cell wall stress; transported to the cell surface after Pmt1p-Pmt2p-mediated O-mannosylation, colocalizing with bud scars
S000004185	YLR195C	NMT1	N-Myristoyl Transferase	541937	543304	-1	N-myristoyl transferase	N-myristoyl transferase; catalyzes the cotranslational, covalent attachment of myristic acid to the N-terminal glycine residue of several proteins involved in cellular growth and signal transduction
S000004186	YLR196W	PWP1	Periodic tryptophan (W) Protein	543968	545698	1	Protein with WD-40 repeats involved in rRNA processing	Protein with WD-40 repeats involved in rRNA processing; associates with rDNA chromatin in H4-tail-dependent manner; associates with trans-acting ribosome biogenesis factors; similar to beta-transducin superfamily
S000004187	YLR197W	NOP56	NucleOlar Protein of 56.8 kDa	546097	547611	1	Essential evolutionarily-conserved nucleolar protein	Essential evolutionarily-conserved nucleolar protein; component of the box C/D snoRNP complexes that direct 2'-O-methylation of pre-rRNA during its maturation; overexpression causes spindle orientation defects
S000004188	YLR198C	""	""	547286	547645	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene SIK1/YLR197W
S000004189	YLR199C	PBA1	Proteasome Biogenesis-Associated	547853	548769	-1	Protein involved in 20S proteasome assembly	Protein involved in 20S proteasome assembly; forms a heterodimer with Add66p that binds to proteasome precursors; interaction with Pba1p-Add66p may affect function of the mature proteasome and its role in maintaining respiratory metabolism; similar to human PAC1 constituent of the PAC1-PAC2 complex involved in proteasome assembly
S000004190	YLR200W	YKE2	Yeast ortholog of mouse KE2	549012	549356	1	Subunit of the heterohexameric Gim/prefoldin protein complex	Subunit of the heterohexameric Gim/prefoldin protein complex; involved in the folding of alpha-tubulin, beta-tubulin, and actin; prefoldin complex also localizes to chromatin of actively transcribed genes in the nucleus and facilitates transcriptional elongation
S000004191	YLR201C	COQ9	COenzyme Q	549511	550293	-1	Protein required for ubiquinone biosynthesis and respiratory growth	Protein required for ubiquinone biosynthesis and respiratory growth; localizes to matrix face of mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes; ubiquinone is also known as coenzyme Q; human homolog COQ9 can complement yeast coq9 null mutant
S000004192	YLR202C	""	""	550194	550636	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YLR201C; ORF contains a putative intron
S000004193	YLR203C	MSS51	Mitochondrial Splicing Suppressor	550650	551960	-1	Specific translational activator for the mitochondrial COX1 mRNA	Specific translational activator for the mitochondrial COX1 mRNA; loosely associated with the matrix face of the mitochondrial inner membrane; localizes to vacuole membrane in response to H2O2; influences both COX1 mRNA translation and Cox1p assembly into cytochrome c oxidase; binds to heme B, which may be a mechanism for sensing oxygen levels in order to regulate cytochrome c oxidase biogenesis
S000004194	YLR204W	QRI5	Quasi-Renownless Information	552270	552605	1	Mitochondrial inner membrane protein	Mitochondrial inner membrane protein; required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA
S000004195	YLR205C	HMX1	HeMe oXygenase	552724	553677	-1	ER localized heme oxygenase	ER localized heme oxygenase; involved in heme degradation during iron starvation and in the oxidative stress response; expression is regulated by AFT1 and oxidative stress; relocates to the perinuclear region in the presence of oxidants
S000004196	YLR206W	ENT2	Epsin N-Terminal homology	554578	556419	1	Epsin-like protein required for endocytosis and actin patch assembly	Epsin-like protein required for endocytosis and actin patch assembly; K48-, K63-specific Ub chain binding protein that functions as an endocytic adaptor; contains clathrin-binding motif at C-terminus; functionally redundant with Ent1p; ENT2 has a paralog, ENT1, that arose from the whole genome duplication
S000004197	YLR207W	HRD3	HMG-coA Reductase Degradation	556788	559289	1	ER membrane protein that plays a central role in ERAD	ER membrane protein that plays a central role in ERAD; forms HRD complex with Hrd1p and ER-associated protein degradation (ERAD) determinants that engages in lumen to cytosol communication and coordination of ERAD events
S000004199	YLR209C	PNP1	purine nucleoside phosphorylase	560797	561732	-1	Purine nucleoside phosphorylase	Purine nucleoside phosphorylase; specifically metabolizes inosine and guanosine nucleosides; involved in the nicotinamide riboside salvage pathway
S000004200	YLR210W	CLB4	CycLin B	562008	563390	1	B-type cyclin involved in cell cycle progression	B-type cyclin involved in cell cycle progression; activates Cdc28p to promote the G2/M transition; may be involved in DNA replication and spindle assembly; accumulates during S phase and G2, then targeted for ubiquitin-mediated degradation; CLB4 has a paralog, CLB3, that arose from the whole genome duplication
S000004201	YLR211C	ATG38	AuTophaGy related	563792	564531	-1	Homodimeric subunit of autophagy-specific PtdIns-3-kinase complex I	Homodimeric subunit of autophagy-specific PtdIns-3-kinase complex I; required for the integrity of the active PtdIns-3-kinase complex I by maintaining an association between Vps15p-Vps34p and Atg14p-Vps30p subcomplexes; localizes to the pre-autophagosomal structure (PAS) in an Atg14p-dependent manner; ATG38 is non-essential but is required for macroautophagy
S000004202	YLR212C	TUB4	TUBulin	564860	566281	-1	Gamma-tubulin	Gamma-tubulin; involved in nucleating microtubules from both the cytoplasmic and nuclear faces of the spindle pole body; protein abundance increases in response to DNA replication stress; null mutation is complemented by human TUBG1
S000004203	YLR213C	CRR1	CRH-Related	566654	567922	-1	Putative glycoside hydrolase of the spore wall envelope	Putative glycoside hydrolase of the spore wall envelope; required for normal spore wall assembly, possibly for cross-linking between the glucan and chitosan layers; expressed during sporulation
S000004204	YLR214W	FRE1	Ferric REductase	568567	570627	1	Ferric reductase and cupric reductase	Ferric reductase and cupric reductase; reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low copper and iron levels
S000004205	YLR215C	CDC123	Cell Division Cycle	570776	571858	-1	Assembly factor for the eIF2 translation initiation factor complex	Assembly factor for the eIF2 translation initiation factor complex; regulates translational initiation; conserved residues of this ATP-Grasp protein that bind to ATP-Mg2+ in the pombe ortholog are required for complex assembly in budding yeast; interaction with eIF2 subunit Gcd11p facilitates complex assembly and activity; required for the START transition and timely progression through G2; regulated by nutrient availability; human ortholog complements the yeast mutant
S000004206	YLR216C	CPR6	Cyclosporin-sensitive Proline Rotamase	572096	573211	-1	Peptidyl-prolyl cis-trans isomerase (cyclophilin)	Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; plays a role in determining prion variants; binds to Hsp82p and contributes to chaperone activity; protein abundance increases in response to DNA replication stress
S000004207	YLR217W	""	""	572909	573232	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CPR6
S000004208	YLR218C	COA4	Cytochrome Oxidase Assembly factor	573466	573918	-1	Twin Cx(9)C protein involved in cytochrome c oxidase organization	Twin Cx(9)C protein involved in cytochrome c oxidase organization; organization includes assembly or stability; localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; interacts genetically with CYC1 and with cytochrome c oxidase assembly factors; mutation can be complemented by human COA4
S000004209	YLR219W	MSC3	Meiotic Sister-Chromatid recombination	574151	576337	1	Protein of unknown function	Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; msc3 mutants are defective in directing meiotic recombination events to homologous chromatids; potential Cdc28p substrate; protein abundance increases in response to DNA replication stress
S000004210	YLR220W	CCC1	Cross-Complements Ca(2+) phenotype of csg1	576825	577793	1	Vacuolar Fe2+/Mn2+ transporter	Vacuolar Fe2+/Mn2+ transporter; suppresses respiratory deficit of yfh1 mutants, which lack ortholog of mammalian frataxin, by preventing mitochondrial iron accumulation; relative distribution to vacuole decreases upon DNA replication stress; targeted to vacuole via AP-3 pathway
S000004211	YLR221C	RSA3	RiboSome Assembly	578362	579024	-1	Protein with a likely role in ribosomal maturation	Protein with a likely role in ribosomal maturation; required for accumulation of wild-type levels of large (60S) ribosomal subunits; binds to the helicase Dbp6p in pre-60S ribosomal particles in the nucleolus
S000004212	YLR222C	UTP13	U Three Protein	579318	581771	-1	Nucleolar protein	Nucleolar protein; component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
S000004213	YLR223C	IFH1	Interacts with Fork Head	582233	585490	-1	Coactivator, regulates transcription of ribosomal protein (RP) genes	Coactivator, regulates transcription of ribosomal protein (RP) genes; recruited to RP gene promoters during optimal growth conditions via Fhl1p; subunit of CURI, a complex that coordinates RP production and pre-rRNA processing; regulated by acetylation and phosphorylation at different growth states via TORC1 signaling; IFH1 has a paralog, CRF1, that arose from the whole genome duplication
S000004214	YLR224W	UCC1	Ubiquitination of Citrate synthase in the glyoxylate Cycle	586464	587573	1	F-box protein and component of SCF ubiquitin ligase complexes	F-box protein and component of SCF ubiquitin ligase complexes; involved in ubiquitin-dependent protein catabolism; readily monoubiquitinated in vitro by SCF-Ubc4 complexes; SCF-Ucc1 regulates level of Cit2 citrate synthase protein to maintain citrate homeostasis, acts as metabolic switch for glyoxylate cycle; UCC1 transcription is downregulated in cells grown on C2-compounds
S000004215	YLR225C	""	""	587695	588918	-1	Putative protein of unknown function	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; not an essential gene; YLR225C has a paralog, YDR222W, that arose from the whole genome duplication
S000004216	YLR226W	BUR2	Bypass UAS Requirement	589354	590541	1	Cyclin for the Sgv1p (Bur1p) protein kinase	Cyclin for the Sgv1p (Bur1p) protein kinase; subunit of the CDK-cyclin BUR kinase complex with Sgv1p (Bur1p); regulates Set2p-dependent H3K36 trimethylation; regulates transcriptional elongation through the phosphorylation of the carboxy-terminal domain (CTD) of Rpo21p; involved in the recruitment of Spt6p to the CTD at the onset of transcription; regulates telomere elongation
S000004217	YLR227C	ADY4	Accumulation of DYads	590562	592043	-1	Structural component of the meiotic outer plaque	Structural component of the meiotic outer plaque; outer plaque is a membrane-organizing center that assembles on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane
S000004218	YLR228C	ECM22	ExtraCellular Mutant	600019	602463	-1	Sterol regulatory element binding protein	Sterol regulatory element binding protein; regulates transcription of sterol biosynthetic genes upon sterol depletion, after relocating from intracellular membranes to perinuclear foci; redundant activator of filamentation with UPC2, up-regulating the expression of genes involved in filamentous growth; contains Zn[2]-Cys[6] binuclear cluster; ECM22 has a paralog, UPC2, that arose from the whole genome duplication
S000004219	YLR229C	CDC42	Cell Division Cycle	604212	604787	-1	Small rho-like GTPase	Small rho-like GTPase; essential for establishment and maintenance of cell polarity; plays a role late in cell fusion via activation of key cell fusion regulator Fus2p; acts with ESCRT proteins at sites of nuclear envelope and ER fission; mutants have defects in the organization of actin and septins; human homolog CDC42 can complement yeast cdc42 mutant
S000004220	YLR230W	""	""	604574	604879	1	Dubious open reading frame unlikely to encode a functional protein	Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42/YLR229C gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity
S000004221	YLR231C	BNA5	Biosynthesis of Nicotinic Acid	605758	607119	-1	Kynureninase	Kynureninase; required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p
S000004222	YLR232W	""	""	606830	607177	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene BNA5
S000004223	YLR233C	EST1	Ever Shorter Telomeres	607425	609524	-1	TLC1 RNA-associated factor involved in telomere length regulation	TLC1 RNA-associated factor involved in telomere length regulation; recruitment subunit of telomerase; has G-quadruplex promoting activity required for telomere elongation; role in activating telomere-bound Est2p-TLC1-RNA; EST1 has a paralog, EBS1, that arose from the whole genome duplication
S000004224	YLR234W	TOP3	TOPoisomerase	609783	611753	1	DNA Topoisomerase III	DNA Topoisomerase III; conserved protein that functions in a complex with Sgs1p and Rmi1p to relax single-stranded negatively-supercoiled DNA preferentially; DNA catenation/decatenation activity is stimulated by RPA and Sgs1p-Top3p-Rmi1p; involved in telomere stability and regulation of mitotic recombination
S000004225	YLR235C	""	""	611532	611930	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORFs TOP3/YLR234W and YLR236C
S000004226	YLR236C	""	""	611794	612117	-1	Putative protein of unknown function	Putative protein of unknown function; conserved across S. cerevisiae strains; overlaps ORF YLR235C
S000004227	YLR237W	THI7	THIamine metabolism	612367	614163	1	Plasma membrane transporter responsible for the uptake of thiamine	Plasma membrane transporter responsible for the uptake of thiamine; contributes to uptake of 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside (acadesine); member of the major facilitator superfamily of transporters; mutation of human ortholog causes thiamine-responsive megaloblastic anemia
S000004228	YLR238W	FAR10	Factor ARrest	614755	616191	1	Protein involved in recovery from arrest in response to pheromone	Protein involved in recovery from arrest in response to pheromone; acts in a cell cycle arrest recovery pathway independent from Far1p; interacts with Far3p, Far7p, Far8p, Far9p, and Far11p; potential Cdc28p substrate; FAR10 has a paralog, VPS64, that arose from the whole genome duplication
S000004229	YLR239C	LIP2	LIPoyl ligase	616332	617318	-1	Lipoyl ligase	Lipoyl ligase; involved in the modification of mitochondrial enzymes by the attachment of lipoic acid groups
S000004230	YLR240W	VPS34	Vacuolar Protein Sorting	617533	620160	1	Phosphatidylinositol (PI) 3-kinase that synthesizes PI-3-phosphate	Phosphatidylinositol (PI) 3-kinase that synthesizes PI-3-phosphate; forms membrane-associated signal transduction complex with Vps15p to regulate protein sorting; activated by the GTP-bound form of Gpa1p; a fraction is localized, with Vps15p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery
S000004231	YLR241W	CSC1	Calcium permeable Stress-gated cation Channel	620473	622821	1	Calcium permeable gated cation channel	Calcium permeable gated cation channel; functions as an osmosensitive, calcium-permeable cation channel when expressed in a heterologous system; may be involved in detoxification; similar to Arabidopsis CSC1
S000004232	YLR242C	ARV1	ARE2 Required for Viability	622918	623883	-1	Cortical ER protein	Cortical ER protein; implicated in the membrane insertion of tail-anchored C-terminal single transmembrane domain proteins; may function in transport of glycosylphosphatidylinositol intermediates into the ER lumen; required for normal intracellular sterol distribution; human ARV1, required for normal cholesterol and bile acid homeostasis, can complement yeast arv1 null mutant; human variant causing early onset epileptic encephalopathy is unable to rescue the yeast null
S000004233	YLR243W	GPN3	Gly-Pro-Asn (N) motif	624203	625021	1	Putative GTPase with a role in biogenesis of RNA pol II and polIII	Putative GTPase with a role in biogenesis of RNA pol II and polIII; may be involved in assembly of RNA polymerases II and III and in their transport into the nucleus; may have a role in sister chromatid cohesion; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum and vacuole respectively; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn2p
S000004234	YLR244C	MAP1	Methionine AminoPeptidase	625168	626331	-1	Methionine aminopeptidase	Methionine aminopeptidase; catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map2p; apo-Map1p is activated by the GTP-dependent zinc transferase Zng1p, especially during zinc starvation; interacts with Zng1p via an N-terminal Zn finger domain
S000004236	YLR246W	ERF2	Effect on Ras Function	627118	628197	1	Subunit of a palmitoyltransferase	Subunit of a palmitoyltransferase; this complex adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; mutants partially mislocalize Ras2p to the vacuole; palmitoyltransferase is composed of Erf2p and Shr5p
S000004237	YLR247C	IRC20	Increased Recombination Centers	628684	633354	-1	E3 ubiquitin ligase and putative helicase	E3 ubiquitin ligase and putative helicase; involved in synthesis-dependent strand annealing-mediated homologous recombination; ensures precise end-joining along with Srs2p in the Yku70p/Yku80p/Lig4p-dependent NHEJ pathway; controls 2-micron plasmid copy number by regulating the levels of the Flp1p recombinase; localizes to both the mitochondrion and the nucleus; contains a Snf2/Swi2 family ATPase/helicase and a RING finger domain; null mutant displays increased levels of spontaneous Rad52p foci
S000004238	YLR248W	RCK2	Radiation sensitivity Complementing Kinase	634252	636084	1	Protein kinase involved in response to oxidative and osmotic stress	Protein kinase involved in response to oxidative and osmotic stress; identified as suppressor of S. pombe cell cycle checkpoint mutations; similar to CaM (calmodulin) kinases; RCK2 has a paralog, RCK1, that arose from the whole genome duplication
S000004239	YLR249W	YEF3	Yeast Elongation Factor	636780	639914	1	Translation elongation factor 3	Translation elongation factor 3; contains two ABC cassettes; binds and hydrolyzes ATP; interacts with mRNA independently of translation; involved in frameshifting at stalled ribosomes; YEF3 has a paralog, HEF3, that arose from the whole genome duplication
S000004240	YLR250W	SSP120	Saccharomyces Secretory Protein	640317	641021	1	Protein packaged into COPII vesicles for ER to Golgi trafficking	Protein packaged into COPII vesicles for ER to Golgi trafficking; forms a complex with Emp47p that may function in trafficking plasma membrane glycoproteins through early secretory pathway; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
S000004241	YLR251W	SYM1	Stress-inducible Yeast Mpv17	641464	642057	1	Protein required for ethanol metabolism	Protein required for ethanol metabolism; induced by heat shock and localized to the inner mitochondrial membrane; lysine auxotroph proposed to function in pyrimidine metabolism; homologous to mammalian peroxisomal membrane protein Mpv17; human homolog MPV17 is implicated in hepatocerebral mtDNA depletion syndromes (MDDS), and complements yeast null mutant
S000004242	YLR252W	""	""	641957	642262	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SYM1, a mitochondrial protein involved in ethanol metabolism
S000004243	YLR253W	CQD2	CoQ Distribution	642627	644336	1	Mitochondrial protein involved in lipid homeostasis	Mitochondrial protein involved in lipid homeostasis; involved in mobilization of CoQ from its site of synthesis on the inner mitochondrial membrane to other sites of action; associates with mitochondrial ribosome; involved in mitochondrial morphology; non-essential gene which interacts genetically with MDM10, and other members of ERMES complex; transcription is periodic during metabolic cycle; homologous to human aarF domain containing kinase, ADCK1
S000004244	YLR254C	NDL1	NuDeL homolog	644404	644973	-1	Homolog of nuclear distribution factor NudE	Homolog of nuclear distribution factor NudE; NUDEL; interacts with Pac1p and regulates dynein targeting to microtubule plus ends; localizes to cytosol, nucleus and peroxisomes
S000004245	YLR255C	""	""	645600	645953	-1	Putative protein of unknown function	Putative protein of unknown function; conserved across S. cerevisiae strains
S000004247	YLR257W	""	""	658826	659791	1	Protein of unknown function	Protein of unknown function; protein abundance increases in response to DNA replication stress
S000004248	YLR258W	GSY2	Glycogen SYnthase	660716	662833	1	Glycogen synthase	Glycogen synthase; expression induced by glucose limitation, nitrogen starvation, heat shock, and stationary phase; activity regulated by cAMP-dependent, Snf1p and Pho85p kinases as well as by the Gac1p-Glc7p phosphatase; GSY2 has a paralog, GSY1, that arose from the whole genome duplication; relocalizes from cytoplasm to plasma membrane upon DNA replication stress
S000004249	YLR259C	HSP60	Heat Shock Protein	663284	665002	-1	Tetradecameric mitochondrial chaperonin	Tetradecameric mitochondrial chaperonin; required for ATP-dependent folding of precursor polypeptides and complex assembly; prevents aggregation and mediates protein refolding after heat shock; role in mtDNA transmission; phosphorylated
S000004250	YLR260W	LCB5	Long-Chain Base	665844	667907	1	Minor sphingoid long-chain base kinase	Minor sphingoid long-chain base kinase; possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules; LCB5 has a paralog, LCB4, that arose from the whole genome duplication
S000004251	YLR261C	VPS63	Vacuolar Protein Sorting	668237	668563	-1	Putative protein of unknown function	Putative protein of unknown function; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect; decreased levels of protein in enolase deficient mutant
S000004252	YLR262C	YPT6	Yeast Protein Two	668244	668891	-1	Rab family GTPase	Rab family GTPase; required for endosome-to-Golgi, intra-Golgi retrograde, and retrograde Golgi-to-ER transport; temporarily at the Golgi, dissociating into the cytosol on arrival of the late Golgi GTPase Ypt32p; Golgi-localized form is GTP bound, while cytosolic form is GDP-bound; required for delivery of Atg9p to the phagophore assembly site during autophagy under heat stress, with Ypt6p for starvation induced autophagy and for the CVT pathway; homolog of mammalian Rab6
S000004253	YLR263W	RED1	REDuctional division	670340	672823	1	Protein component of the synaptonemal complex axial elements	Protein component of the synaptonemal complex axial elements; involved in chromosome segregation during the first meiotic division; critical for coupling checkpoint signaling to SC formation; promotes interhomolog recombination by phosphorylating Hop1p; also interacts with Mec3p and Ddc1p
S000004254	YLR264W	RPS28B	Ribosomal Protein of the Small subunit	673131	673334	1	Protein component of the small (40S) ribosomal subunit	Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S28, no bacterial homolog; has an extraribosomal function in autoregulation, in which Rps28Bp binds to a decapping complex via Edc3p, which then binds to RPS28B mRNA leading to its decapping and degradation; RPS28B has a paralog, RPS28A, that arose from the whole genome duplication
S000004255	YLR265C	NEJ1	Nonhomologous End-Joining defective	674427	675455	-1	DNA end-binding protein required for nonhomologous end joining (NHEJ)	DNA end-binding protein required for nonhomologous end joining (NHEJ); subunit of the DNA ligase IV complex involved in initial end binding, intermolecular ligation, and DNA ligase reactivation after completion of repair; phosphorylation by Tpk1p is required for DNA damage induced NHEJ and for nuclear targeting of Nej1p and Lif1p; human homolog NHEJ1 is phosphorylated by human Tpk1p homolog PRKACB, regulating NHEJ; transcriptionally repressed by MAT heterozygosity
S000004256	YLR266C	PDR8	Pleiotropic Drug Resistance	675619	677724	-1	Transcription factor	Transcription factor; targets include ATP-binding cassette (ABC) transporters, major facilitator superfamily transporters, and other genes involved in the pleiotropic drug resistance (PDR) phenomenon; PDR8 has a paralog, YRR1, that arose from the whole genome duplication
S000004257	YLR267W	BOP2	Bypass Of Pam1	678212	679924	1	Protein of unknown function	Protein of unknown function
S000004258	YLR268W	SEC22	SECretory	680200	680844	1	R-SNARE protein	R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog
S000004259	YLR269C	""	""	680866	681216	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000004260	YLR270W	DCS1	DeCapping Scavenger	681186	682238	1	Non-essential hydrolase involved in mRNA decapping	Non-essential hydrolase involved in mRNA decapping; activates Xrn1p; may function in a feedback mechanism to regulate deadenylation, contains pyrophosphatase activity and a HIT (histidine triad) motif; acts as inhibitor of neutral trehalase Nth1p; required for growth on glycerol medium; protein abundance increases in response to DNA replication stress; DCS1 has a paralog, DCS2, that arose from the whole genome duplication
S000004261	YLR271W	CMG1	Cytoplasmic and Mitochondrial G-patch protein 1	682737	683561	1	Putative protein of unknown function	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS
S000004262	YLR272C	YCS4	Yeast Condensin Subunit	683672	687202	-1	Subunit of the condensin complex	Subunit of the condensin complex; required for establishment and maintenance of chromosome condensation, chromosome segregation, chromatin binding of condensin, tRNA gene clustering at the nucleolus, and silencing at the mating type locus; required for replication slow zone (RSZ) breakage following Mec1p inactivation
S000004263	YLR273C	PIG1	Protein Interacting with Gsy2p	689083	691029	-1	Putative targeting subunit for type-1 protein phosphatase Glc7p	Putative targeting subunit for type-1 protein phosphatase Glc7p; tethers Glc7p to Gsy2p glycogen synthase; PIG1 has a paralog, GAC1, that arose from the whole genome duplication
S000004264	YLR274W	MCM5	MiniChromosome Maintenance	691555	693882	1	Component of the Mcm2-7 hexameric helicase complex	Component of the Mcm2-7 hexameric helicase complex; MCM complex is important for priming origins of DNA replication in G1 and becomes an active ATP-dependent helicase that promotes DNA melting and elongation when activated by Cdc7p-Dbf4p in S-phase
S000004266	YLR276C	DBP9	Dead Box Protein	695046	696830	-1	DEAD-box protein required for 27S rRNA processing	DEAD-box protein required for 27S rRNA processing; exhibits DNA, RNA and DNA/RNA helicase activities; ATPase activity shows preference for DNA over RNA; DNA helicase activity abolished by mutation in RNA-binding domain
S000004267	YLR277C	YSH1	Yeast Seventy-three Homolog	697156	699495	-1	Endoribonuclease	Endoribonuclease; subunit of the mRNA cleavage and polyadenylation specificity complex; required for 3' processing, splicing, and transcriptional termination of mRNAs and snoRNAs; protein abundance increases in response to DNA replication stress; YSH1 has a paralog, SYC1, that arose from the whole genome duplication
S000004268	YLR278C	""	""	699999	704024	-1	Zinc-cluster protein	Zinc-cluster protein; GFP-fusion protein localizes to the nucleus; mutant shows moderate growth defect on caffeine; has a prion-domain like fragment that increases frequency of [URE3]; associates with rDNA chromatin in vivo; YLR278C is not an essential gene
S000004269	YLR279W	""	""	704309	704698	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000004270	YLR280C	""	""	704483	704833	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000004271	YLR281C	RSO55	mitochondrial protein Related to Spastic paraplegia with Optic atrophy and neuropathy SPG55	704493	704960	-1	Stalled-ribosome rescue factor srRF2	Stalled-ribosome rescue factor srRF2; mitochondrial protein required for respiratory growth in presence of bacteriostatic ribosome-binding antibiotics; not an essential gene
S000004272	YLR282C	""	""	705071	705412	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition
S000004273	YLR283W	PUT7	Proline UTilization	705186	706130	1	Regulator of mitochondrial proline metabolism	Regulator of mitochondrial proline metabolism; tethered with Fmp32p to inner mitochondrial membrane in large hetero-oligomeric complex, abundance of which is regulated by proline; involved in mitochondrial proline homeostasis and cellular redox balance; null exhibits pronounced defect in proline utilization, and can be functionally complemented by expression of human homolog MCUR1
S000004274	YLR284C	ECI1	Enoyl-CoA Isomerase	706198	707040	-1	Peroxisomal delta3,delta2-enoyl-CoA isomerase	Peroxisomal delta3,delta2-enoyl-CoA isomerase; hexameric protein that converts 3-hexenoyl-CoA to trans-2-hexenoyl-CoA, essential for the beta-oxidation of unsaturated fatty acids, oleate-induced; ECI1 has a paralog, DCI1, that arose from the whole genome duplication
S000004275	YLR285W	NNT1	Nicotinamide N-methylTransferase	707360	708145	1	S-adenosylmethionine-dependent methyltransferase	S-adenosylmethionine-dependent methyltransferase; novel N-terminal protein methyltransferase that trimethylates the N-terminal glycine residue (G2) and also dimethylates lysine (K3) on elongation factor eEF1A (Tef1p/Tef2p); has a role in rDNA silencing and in lifespan determination
S000004276	YLR286C	CTS1	ChiTinaSe	708448	710136	-1	Endochitinase	Endochitinase; required for cell separation after mitosis; transcriptional activation during the G1 phase of the cell cycle is mediated by transcription factor Ace2p
S000004277	YLR287C	""	""	710991	712058	-1	Putative protein of unknown function	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR287C is not an essential gene
S000004278	YLR287C-A	RPS30A	Ribosomal Protein of the Small subunit	712537	713158	-1	Protein component of the small (40S) ribosomal subunit	Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30A has a paralog, RPS30B, that arose from the whole genome duplication
S000004279	YLR288C	MEC3	Mitosis Entry Checkpoint	713480	714904	-1	DNA damage and meiotic pachytene checkpoint protein	DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1
S000004280	YLR289W	GUF1	Gtpase of Unknown Function	715089	717026	1	Mitochondrial matrix GTPase	Mitochondrial matrix GTPase; associates with mitochondrial ribosomes; important for translation under temperature and nutrient stress; may have a role in translational fidelity; similar to bacterial LepA elongation factor
S000004281	YLR290C	COQ11	COenzyme Q	717145	717978	-1	Putative oxidoreductase, subunit of Coenzyme Q biosynthetic complexes	Putative oxidoreductase, subunit of Coenzyme Q biosynthetic complexes; required for synthesis of wild-type levels of Coenzyme Q (ubiquinone); member of the short-chain dehydrogenase/reductase (SDR) superfamily; orthologous gene in some other fungi is fused to the COQ10 ortholog
S000004282	YLR291C	GCD7	General Control Derepressed	718317	719462	-1	Beta subunit of translation initiation factor eIF2B	Beta subunit of translation initiation factor eIF2B; the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as negative regulator of GCN4 expression; mutations in human ortholog EIF2B2 cause fatal brain disease childhood ataxia with central nervous system hypomyelination (CACH); human ortholog can complement yeast mutant, allows growth down-regulation of yeast gene
S000004283	YLR292C	SEC72	SECretory	719789	720370	-1	Non-essential subunit of Sec63 complex	Non-essential subunit of Sec63 complex; with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and translocation into the ER; other members are Sec63p, Sec62p, and Sec66p
S000004285	YLR294C	""	""	721701	722030	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene ATP14
S000004286	YLR295C	ATP14	ATP synthase	721999	722373	-1	Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase	Subunit h of the F0 sector of mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; protein abundance increases in response to DNA replication stress
S000004287	YLR296W	""	""	722978	723304	1	Putative protein of unknown function	Putative protein of unknown function; conserved across S. cerevisiae strains
S000004288	YLR297W	""	""	724044	724433	1	Protein of unknown function	Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; not an essential gene; induced by treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from nucleus to vacuole upon DNA replication stress; YLR297W has a paralog, YOR186W, that arose from the whole genome duplication
S000004289	YLR298C	YHC1	Yeast Homolog of human U1C	724721	725416	-1	Component of the U1 snRNP complex required for pre-mRNA splicing	Component of the U1 snRNP complex required for pre-mRNA splicing; putative ortholog of human U1C protein, which is involved in formation of a complex between U1 snRNP and the pre-mRNA 5' splice site
S000004290	YLR299W	ECM38	ExtraCellular Mutant	726069	728051	1	Gamma-glutamyltranspeptidase	Gamma-glutamyltranspeptidase; major glutathione-degrading enzyme; involved in detoxification of electrophilic xenobiotics; expression induced mainly by nitrogen starvation
S000004291	YLR300W	EXG1	EXo-1,3-beta-Glucanase	728955	730301	1	Major exo-1,3-beta-glucanase of the cell wall	Major exo-1,3-beta-glucanase of the cell wall; involved in cell wall beta-glucan assembly; exists as three differentially glycosylated isoenzymes; EXG1 has a paralog, SPR1, that arose from the whole genome duplication
S000004294	YLR303W	MET17	METhionine requiring	732542	733876	1	O-acetyl homoserine-O-acetyl serine sulfhydrylase	O-acetyl homoserine-O-acetyl serine sulfhydrylase; involved in methionine and cysteine biosynthesis; long considered essential for inorganic sulfur assimilation, but inefficient homocysteine synthase Hsu1p enables met17 null cells to assimilate enough inorganic sulfur for survival and proliferation; growth failure of met17 null can be explained by toxic accumulation of H2S gas due to a metabolic bottleneck
S000004295	YLR304C	ACO1	ACOnitase	735212	737548	-1	Aconitase	Aconitase; required for the tricarboxylic acid (TCA) cycle and also independently required for mitochondrial genome maintenance; component of the mitochondrial nucleoid; mutation leads to glutamate auxotrophy; mutation in human homolog ACO2 is associated with dominant optic nerve atrophy; human homolog ACO2 can complement yeast null mutant 
S000004296	YLR305C	STT4	STaurosporine and Temperature sensitive	738161	743863	-1	Phosphatidylinositol-4-kinase	Phosphatidylinositol-4-kinase; functions in Pkc1p protein kinase pathway; required for normal vacuole morphology, cell wall integrity, and actin cytoskeleton organization; required for autophagosome-vacuole fusion during autophagy and for lipophagy in stationary phase cells and during nitrogen starvation; localizes to plasma membrane and mitochondria in HTP studies 
S000004297	YLR306W	UBC12	UBiquitin-Conjugating	744151	744851	1	Enzyme that mediates the conjugation of Rub1p	Enzyme that mediates the conjugation of Rub1p; a ubiquitin-like protein, to other proteins; related to E2 ubiquitin-conjugating enzymes
S000004298	YLR307W	CDA1	Chitin DeAcetylase	745620	746525	1	Chitin deacetylase	Chitin deacetylase; together with Cda2p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall
S000004299	YLR308W	CDA2	Chitin DeAcetylase	747937	748875	1	Chitin deacetylase	Chitin deacetylase; together with Cda1p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall
S000004300	YLR309C	IMH1	shares with Integrins and Myosins significant Homology	749034	751769	-1	Protein involved in vesicular transport	Protein involved in vesicular transport; mediates transport between an endosomal compartment and the Golgi, contains a Golgi-localization (GRIP) domain that interacts with activated Arl1p-GTP to localize Imh1p to the Golgi
S000004301	YLR310C	CDC25	Cell Division Cycle	752224	756993	-1	Membrane bound guanine nucleotide exchange factor	Membrane bound guanine nucleotide exchange factor; also known as a GEF or GDP-release factor; indirectly regulates adenylate cyclase through activation of Ras1p and Ras2p by stimulating the exchange of GDP for GTP; required for progression through G1; thermosensitivity of the cdc25-5 mutant is functionally complemented by human RASGRF1 or by a fragment of human SOS1 comprising the CDC25-related catalytic domain
S000004302	YLR311C	""	""	757265	757612	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000004303	YLR312C	ATG39	AuTophaGy related	757637	758833	-1	Transmembrane receptor with a role in nucleophagy	Transmembrane receptor with a role in nucleophagy; involved in selective autophagy of the endoplasmic reticulum and nucleus in response to nitrogen starvation or rapamycin treatment; role in nuclear envelope deformation to generate nucleus-derived double-membrane vesicles (NDVs), interacting with Atg8p to target these NDVs to forming autophagosomal membranes; anchored in the outer nuclear membrane, associating with the inner nuclear membrane via amphipathic helices
S000004304	YLR312W-A	MRPL15	Mitochondrial Ribosomal Protein, Large subunit	759480	760241	1	Mitochondrial ribosomal protein of the large subunit	Mitochondrial ribosomal protein of the large subunit
S000004305	YLR313C	SPH1	SPa2 Homolog	760750	762342	-1	Protein involved in shmoo formation and bipolar bud site selection	Protein involved in shmoo formation and bipolar bud site selection; localizes to sites of polarized growth in a cell cycle dependent- and Spa2p-dependent manner, interacts with MAPKKs Mkk1p, Mkk2p, and Ste7p; SPH1 has a paralog, SPA2, that arose from the whole genome duplication
S000004306	YLR314C	CDC3	Cell Division Cycle	762575	764137	-1	Component of the septin ring that is required for cytokinesis	Component of the septin ring that is required for cytokinesis; septins are GTP-binding proteins that assemble with other septins into rod-like complexes that can associate with other rods to form filament polymers; septin rings at the mother-bud neck act as scaffolds for recruiting factors needed for cell division and as barriers to prevent diffusion of specific proteins between mother and daughter cells
S000004307	YLR315W	NKP2	Non-essential Kinetochore Protein	764808	765269	1	Central kinetochore protein and subunit of the Ctf19 complex	Central kinetochore protein and subunit of the Ctf19 complex; mutants have elevated rates of chromosome loss; orthologous to fission yeast kinetochore protein cnl2
S000004308	YLR316C	TAD3	tRNA-specific Adenosine Deaminase	765266	766358	-1	Subunit of tRNA-specific adenosine-34 deaminase	Subunit of tRNA-specific adenosine-34 deaminase; forms a heterodimer with Tad2p that converts adenosine to inosine at the wobble position of several tRNAs; localizes to cytosol, nucleus and peroxisomes
S000004309	YLR317W	""	""	765655	766089	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; may be part of a bicistronic transcript with NKP2/YLR315W; overlaps the verified ORF TAD3/YLR316C
S000004310	YLR318W	EST2	Ever Shorter Telomeres	766542	769196	1	Reverse transcriptase subunit of the telomerase holoenzyme	Reverse transcriptase subunit of the telomerase holoenzyme; essential for telomerase core catalytic activity, involved in other aspects of telomerase assembly and function; mutations in human homolog are associated with aplastic anemia
S000004311	YLR319C	BUD6	BUD site selection	769318	771684	-1	Actin- and formin-interacting protein	Actin- and formin-interacting protein; nucleation-promoting factor (NPF) for Bni1p and Bnr1p during actin cable assembly and organization; enhances Bnr1p-mediated actin nucleation, alleviating Hof1p inhibition through competitive binding; interacts with Bni1p via a C-terminal triple helical coiled-coil domain; role in polarized cell growth; isolated as a bipolar budding mutant; localizes to the presumptive bud site, small bud tips, the bud neck and secretory vesicles; potential Cdc28p substrate
S000004312	YLR320W	MMS22	Methyl MethaneSulfonate sensitivity	771940	776304	1	Subunit of E3 ubiquitin ligase complex involved in replication repair	Subunit of E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of replication fork, such as fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; Rtt101p-Mms22p ligase associates with replisome complex during S phase via Ctf4p; required for accurate meiotic chromosome segregation
S000004313	YLR321C	SFH1	Snf Five Homolog	776584	777864	-1	Component of the RSC chromatin remodeling complex	Component of the RSC chromatin remodeling complex; essential gene required for cell cycle progression and maintenance of proper ploidy; phosphorylated in the G1 phase of the cell cycle; Snf5p paralog; hSNF5 tumor suppressor ortholog
S000004314	YLR322W	VPS65	Vacuolar Protein Sorting	777628	777942	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 75% of ORF overlaps the verified gene SFH1; deletion causes a vacuolar protein sorting defect and blocks anaerobic growth
S000004315	YLR323C	CWC24	Complexed With Cef1p	778173	778952	-1	General splicing factor	General splicing factor; required for stable U2 snRNP binding to primary transcripts; essential for the first step of splicing; component of the pre-catalytic spliceosome complex containing Cef1p; similar to S. pombe Cwf24p
S000004316	YLR324W	PEX30	PEroXisome related	779215	780786	1	ER-resident protein involved in peroxisomal biogenesis	ER-resident protein involved in peroxisomal biogenesis; ER-localized protein that associates with peroxisomes; interacts with Pex29p and reticulons Rtn1p and Yop1p to regulate peroxisome biogenesis from the ER; role in peroxisomal-destined vesicular flow from the ER; partially redundant with Pex31p; may function at a step downstream of steps mediated by Pex28p and Pex29p; PEX30 has a paralog, PEX31, that arose from the whole genome duplication
S000004317	YLR325C	RPL38	Ribosomal Protein of the Large subunit	781143	781379	-1	Ribosomal 60S subunit protein L38	Ribosomal 60S subunit protein L38; homologous to mammalian ribosomal protein L38, no bacterial homolog
S000004318	YLR326W	""	""	782174	782896	1	Putative protein of unknown function	Putative protein of unknown function; SWAT-GFP and mCherry fusion proteins localize to the cell periphery; predicted to be palmitoylated
S000004319	YLR327C	TMA10	Translation Machinery Associated	783127	783387	-1	Protein of unknown function that associates with ribosomes	Protein of unknown function that associates with ribosomes; protein abundance increases in response to DNA replication stress; TMA10 has a paralog, STF2, that arose from the whole genome duplication
S000004320	YLR328W	NMA1	Nicotinamide Mononucleotide Adenylyltransferase	784913	786118	1	Nicotinic acid mononucleotide adenylyltransferase	Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in pathways of NAD biosynthesis, including the de novo, NAD(+) salvage, and nicotinamide riboside salvage pathways; homolog of human NMNAT; NMA1 has a paralog, NMA2, that arose from the whole genome duplication
S000004321	YLR329W	REC102	RECombination	786442	787333	1	Protein involved in early stages of meiotic recombination	Protein involved in early stages of meiotic recombination; required for chromosome synapsis; forms a complex with Rec104p and Spo11p necessary during the initiation of recombination
S000004322	YLR330W	CHS5	CHitin Synthase-related	787664	789679	1	Component of the exomer complex	Component of the exomer complex; the exomer which also contains Csh6p, Bch1p, Bch2p, and Bud7, is involved in the export of select proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane; interacts selectively with the activated, GTP-bound form of Arf1p; Chs5p is the only protein with a BRCT domain that is not localized to the nucleus
S000004323	YLR331C	JIP3	Jumonji domain Interacting Protein	790669	791046	-1	Putative protein of unknown function	Putative protein of unknown function; conserved among S. cerevisiae strains; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
S000004324	YLR332W	MID2	Mating pheromone-Induced Death	790676	791806	1	O-glycosylated plasma membrane protein	O-glycosylated plasma membrane protein; acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p; MID2 has a paralog, MTL1, that arose from the whole genome duplication
S000004325	YLR333C	RPS25B	Ribosomal Protein of the Small subunit	795573	795899	-1	Protein component of the small (40S) ribosomal subunit	Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S25, no bacterial homolog; RPS25B has a paralog, RPS25A, that arose from the whole genome duplication
S000004326	YLR334C	""	""	796698	797078	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps a stand-alone long terminal repeat sequence whose presence indicates a retrotransposition event occurred here
S000004327	YLR335W	NUP2	NUclear Pore	797430	799592	1	Nucleoporin involved in nucleocytoplasmic transport	Nucleoporin involved in nucleocytoplasmic transport; binds to either the nucleoplasmic or cytoplasmic faces of the nuclear pore complex depending on Ran-GTP levels; also has a role in chromatin organization; involved with Nup60p in nuclear basket remodeling during meiosis; Cdc5p-mediated phosphorylation of Nup60p leads to detachment and reattachment of nuclear basket
S000004328	YLR336C	SGD1	Suppressor of Glycerol Defect	799697	802396	-1	Essential nuclear protein	Essential nuclear protein; required for biogenesis of the small ribosomal subunit; has a possible role in the osmoregulatory glycerol response; putative homolog of human NOM1 which is implicated in acute myeloid leukemia
S000004329	YLR337C	VRP1	VeRProlin	802653	805106	-1	Verprolin, proline-rich actin-associated protein	Verprolin, proline-rich actin-associated protein; involved in cytoskeletal organization and cytokinesis; promotes actin nucleation and endocytosis; related to mammalian Wiskott-Aldrich syndrome protein (WASP)-interacting protein (WIP)
S000004330	YLR338W	OPI9	OverProducer of Inositol	804346	805203	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF VRP1/YLR337C
S000004331	YLR339C	""	""	805784	806335	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the essential gene RPP0
S000004333	YLR341W	SPO77	SPOrulation	807385	808818	1	Meiosis-specific protein of unknown function	Meiosis-specific protein of unknown function; required for spore wall formation during sporulation and for timely prospore membrane closure along with SPS1; required with Sps1p for phosphorylation and turnover of Ssp1p; dispensable for both nuclear divisions during meiosis
S000004334	YLR342W	FKS1	FK506 Sensitivity	809997	815627	1	Catalytic subunit of 1,3-beta-D-glucan synthase	Catalytic subunit of 1,3-beta-D-glucan synthase; transfers glucose from UDP-glucose to the growing chain of glucan via β-1,3-glycosidic linkages, translocating the polymerized β-1,3-glucan to the extracellular space; involved in cell wall synthesis and maintenance; functionally redundant with alternate catalytic subunit Gsc2p; activity regulated by Rho1p, a subunit of the synthase complex; localizes to sites of cell wall remodeling; contains a cellulose-synthase-like fold; antifungal drug target
S000004335	YLR343W	GAS2	Glycophospholipid-Anchored Surface protein	816094	817761	1	1,3-beta-glucanosyltransferase	1,3-beta-glucanosyltransferase; involved with Gas4p in spore wall assembly; has similarity to Gas1p
S000004336	YLR344W	RPL26A	Ribosomal Protein of the Large subunit	819312	820142	1	Ribosomal 60S subunit protein L26A	Ribosomal 60S subunit protein L26A; binds to 5.8S rRNA; non-essential even when paralog is also deleted; deletion has minimal affections on ribosome biosynthesis; homologous to mammalian ribosomal protein L26 and bacterial L24; RPL26A has a paralog, RPL26B, that arose from the whole genome duplication
S000004337	YLR345W	""	""	820511	822040	1	Similar to 6-phosphofructo-2-kinase enzymes	Similar to 6-phosphofructo-2-kinase enzymes; mRNA expression is repressed by the Rfx1p-Tup1p-Ssn6p repressor complex; YLR345W is not an essential gene
S000004338	YLR346C	CIS1	CItrinin Sensitive knockout	822287	822592	-1	Protein of unknown function found in mitochondria	Protein of unknown function found in mitochondria; expression is regulated by transcription factors involved in pleiotropic drug resistance, Pdr1p and Yrr1p; not an essential gene; YLR346C has a paralog, YGR035C, that arose from the whole genome duplication
S000004339	YLR347C	KAP95	KAryoPherin	823828	826413	-1	Karyopherin beta	Karyopherin beta; forms a complex with Srp1p/Kap60p; interacts with nucleoporins to mediate nuclear import of NLS-containing cargo proteins via the nuclear pore complex; regulates PC biosynthesis; GDP-to-GTP exchange factor for Gsp1p
S000004340	YLR348C	DIC1	DIcarboxylate Carrier	826977	827873	-1	Mitochondrial dicarboxylate carrier	Mitochondrial dicarboxylate carrier; integral membrane protein, catalyzes a dicarboxylate-phosphate exchange across the inner mitochondrial membrane, transports cytoplasmic dicarboxylates into the mitochondrial matrix
S000004341	YLR349W	""	""	827521	828027	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified ORF DIC1/YLR348C
S000004342	YLR350W	ORM2	""	828730	829380	1	Protein that mediates sphingolipid homeostasis	Protein that mediates sphingolipid homeostasis; evolutionarily conserved, required for resistance to agents that induce unfolded protein response; Orm1p and Orm2p together control membrane biogenesis by coordinating lipid homeostasis with protein quality control; protein abundance increases in response to DNA replication stress; ORM2 has a paralog, ORM1, that arose from the whole genome duplication
S000004343	YLR351C	NIT3	NITrilase superfamily	829489	830364	-1	Nit protein	Nit protein; one of two proteins in S. cerevisiae with similarity to the Nit domain of NitFhit from fly and worm and to the mouse and human Nit protein which interacts with the Fhit tumor suppressor; nitrilase superfamily member
S000004344	YLR352W	LUG1	Lets [URE3]/ure2 Grow	831116	833539	1	Protein of unknown function with similarity to F-box proteins	Protein of unknown function with similarity to F-box proteins; interacts with the core SCF ubiquitin ligase complex subunits Skp1p and Cdc53p; LUG1 deletion prevents cells containing the [URE3] prion or lacking Ure2p from growing on glycerol
S000004345	YLR353W	BUD8	BUD site selection	834352	836163	1	Protein involved in bud-site selection	Protein involved in bud-site selection; diploid mutants display a unipolar budding pattern instead of the wild-type bipolar pattern, and bud at the proximal pole; BUD8 has a paralog, BUD9, that arose from the whole genome duplication
S000004346	YLR354C	TAL1	TransALdolase	836350	837357	-1	Transaldolase, enzyme in the non-oxidative pentose phosphate pathway	Transaldolase, enzyme in the non-oxidative pentose phosphate pathway; converts sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate to erythrose 4-phosphate and fructose 6-phosphate; TAL1 has a paralog, NQM1, that arose from the whole genome duplication
S000004347	YLR355C	ILV5	IsoLeucine-plus-Valine requiring	838066	839253	-1	Acetohydroxyacid reductoisomerase and mtDNA binding protein	Acetohydroxyacid reductoisomerase and mtDNA binding protein; involved in branched-chain amino acid biosynthesis and maintenance of wild-type mitochondrial DNA; found in mitochondrial nucleoids
S000004348	YLR356W	ATG33	AuTophaGy related	840321	840914	1	Mitochondrial mitophagy-specific protein	Mitochondrial mitophagy-specific protein; required primarily for mitophagy induced at post-log phase; not required for other types of selective autophagy or macroautophagy; conserved within fungi, but not in higher eukaryotes; ATG33 has a paralog, SCM4, that arose from the whole genome duplication
S000004349	YLR357W	RSC2	Remodel the Structure of Chromatin	841331	844000	1	Component of the RSC chromatin remodeling complex	Component of the RSC chromatin remodeling complex; required for expression of mid-late sporulation-specific genes; involved in telomere maintenance; RSC2 has a paralog, RSC1, that arose from the whole genome duplication
S000004350	YLR358C	""	""	843487	844050	-1	Protein of unknown function	Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps ORF RSC2/YLR357W
S000004351	YLR359W	ADE13	ADEnine requiring	844282	845730	1	Adenylosuccinate lyase	Adenylosuccinate lyase; catalyzes two steps in the 'de novo' purine nucleotide biosynthetic pathway; expression is repressed by adenine and activated by Bas1p and Pho2p; mutations in human ortholog ADSL cause adenylosuccinase deficiency; human ADSL can complement yeast ADE13 null mutant
S000004352	YLR360W	VPS38	Vacuolar Protein Sorting	846103	847422	1	Part of a Vps34p phosphatidylinositol 3-kinase complex	Part of a Vps34p phosphatidylinositol 3-kinase complex; functions in carboxypeptidase Y (CPY) sorting; binds Vps30p and Vps34p to promote production of phosphatidylinositol 3-phosphate (PtdIns3P) which stimulates kinase activity; required for overflow degradation of misfolded proteins when ERAD is saturated; targeted to vacuole via AP-3 pathway
S000004353	YLR361C	DCR2	Dose-dependent Cell cycle Regulator	847388	849124	-1	Protein phosphatase	Protein phosphatase; involved in downregulation of the unfolded protein response (UPR), at least in part through dephosphorylation of Ire1p; dosage-dependent positive regulator of the G1/S phase transition through control of the timing of START; physically interacts with, dephosphorylates and destabilizes Sic1p; SWAT-GFP and mCherry fusion proteins localize to the vacuole
S000004354	YLR362W	STE11	STErile	849866	852019	1	Signal transducing MEK kinase	Signal transducing MEK kinase; involved in pheromone response and pseudohyphal/invasive growth pathways where it phosphorylates Ste7p, and the high osmolarity response pathway, via phosphorylation of Pbs2p; regulated by Ste20p and Ste50p; protein abundance increases in response to DNA replication stress
S000004355	YLR363C	NMD4	Nonsense-Mediated mRNA Decay	852495	853151	-1	Protein that may be involved in nonsense-mediated mRNA decay	Protein that may be involved in nonsense-mediated mRNA decay; interacts with Nam7p, relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
S000004356	YLR364W	GRX8	GlutaRedoXin	854062	854391	1	Glutaredoxin that employs a dithiol mechanism of catalysis	Glutaredoxin that employs a dithiol mechanism of catalysis; monomeric; activity is low and null mutation does not affect sensitivity to oxidative stress; GFP-fusion protein localizes to the cytoplasm; expression strongly induced by arsenic
S000004357	YLR365W	""	""	855199	855531	1	Putative protein of unknown function	Putative protein of unknown function; conserved among S. cerevisiae strains; YLR365W is not an essential gene
S000004358	YLR366W	""	""	855538	855843	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YLR364C-A
S000004359	YLR367W	RPS22B	Ribosomal Protein of the Small subunit	856442	857317	1	Protein component of the small (40S) ribosomal subunit	Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S15A and bacterial S8; RPS22B has a paralog, RPS22A, that arose from the whole genome duplication
S000004360	YLR368W	MDM30	Mitochondrial Distribution and Morphology	857540	859336	1	F-box component of an SCF ubiquitin protein ligase complex	F-box component of an SCF ubiquitin protein ligase complex; associates with and is required for Fzo1p ubiquitination and for mitochondria fusion; stimulates nuclear export of specific mRNAs; promotes ubiquitin-mediated degradation of Gal4p in some strains
S000004361	YLR369W	SSQ1	Stress-Seventy subfamily Q	859552	861525	1	Mitochondrial hsp70-type molecular chaperone	Mitochondrial hsp70-type molecular chaperone; required for assembly of iron/sulfur clusters into proteins at a step after cluster synthesis; involved in maturation of Grx5p and Yfh1p, which is a homolog of human frataxin implicated in Friedreich's ataxia
S000004362	YLR370C	ARC18	ARp2/3 Complex subunit	861718	862254	-1	Subunit of the ARP2/3 complex	Subunit of the ARP2/3 complex; ARP2/3 is required for the motility and integrity of cortical actin patches
S000004363	YLR371W	ROM2	RhO1 Multicopy suppressor	862714	866784	1	Guanine nucleotide exchange factor (GEF) for Rho1p and Rho2p	Guanine nucleotide exchange factor (GEF) for Rho1p and Rho2p; mutations are synthetically lethal with mutations in rom1, which also encodes a GEF; Rom2p localization to the bud surface is dependent on Ack1p; ROM2 has a paralog, ROM1, that arose from the whole genome duplication
S000004364	YLR372W	ELO3	fatty acid ELOngation	867354	868391	1	Elongase	Elongase; involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis; lethality of the elo2 elo3 double null mutation is functionally complemented by human ELOVL1 and weakly complemented by human ELOVL3 or ELOV7
S000004365	YLR373C	VID22	Vacuolar Import and Degradation	868662	871367	-1	G-quadruplex (G4) DNA binding protein	G-quadruplex (G4) DNA binding protein; maintains genome integrity by promoting the stability of G4-DNA regions; involved in nucleosome displacement during double-strand break repair; involved in fructose-1,6-bisphosphatase (FBPase) transport from the cytosol to Vid (vacuole import and degradation) vesicles during degradation; glycosylated integral membrane protein of the plasma membrane; localizes to the nucleus; contains a BED-type zinc finger domain and a RNase H-like domain
S000004366	YLR374C	""	""	871453	871842	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF STP3/YLR375W
S000004367	YLR375W	STP3	protein with similarity to Stp1p	871697	872728	1	Zinc-finger protein of unknown function	Zinc-finger protein of unknown function; possibly involved in pre-tRNA splicing and in uptake of branched-chain amino acids; STP3 has a paralog, STP4, that arose from the whole genome duplication
S000004368	YLR376C	PSY3	Platinum SensitivitY	872826	873554	-1	Component of Shu complex (aka PCSS complex)	Component of Shu complex (aka PCSS complex); Shu complex also includes Shu1, Csm2, Shu2, and promotes error-free DNA repair; promotes Rad51p filament assembly; Shu complex mediates inhibition of Srs2p function; Psy3p and Csm2p contain similar DNA-binding regions which work together to form a single DNA binding site; deletion of PSY3 results in a mutator phenotype; deletion increases sensitivity to anticancer drugs oxaliplatin and cisplatin but not mitomycin C
S000004369	YLR377C	FBP1	Fructose-1,6-BisPhosphatase	873746	874792	-1	Fructose-1,6-bisphosphatase	Fructose-1,6-bisphosphatase; key regulatory enzyme in the gluconeogenesis pathway, required for glucose metabolism; undergoes either proteasome-mediated or autophagy-mediated degradation depending on growth conditions; glucose starvation results in redistribution to the periplasm; interacts with Vid30p
S000004370	YLR378C	SEC61	SECretory	875736	877178	-1	Conserved ER protein translocation channel	Conserved ER protein translocation channel; essential subunit of Sec61 complex (Sec61p, Sbh1p, and Sss1p); forms channel for SRP-dependent protein import; with Sec63 complex is required for SRP-independent protein translocation into the ER; involved in posttranslational soluble protein import into the ER, ERAD of soluble substrates, and misfolded soluble protein export from the ER
S000004371	YLR379W	""	""	876922	877296	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the essential ORF SEC61/YLR378C
S000004372	YLR380W	CSR1	Chs5 Spa2 Rescue	878282	879508	1	Phosphatidylinositol transfer protein	Phosphatidylinositol transfer protein; has a potential role in regulating lipid and fatty acid metabolism under heme-depleted conditions; interacts specifically with thioredoxin peroxidase; may have a role in oxidative stress resistance; protein abundance increases in response to DNA replication stress
S000004373	YLR381W	CTF3	Chromosome Transmission Fidelity	879723	881924	1	Outer kinetochore protein that forms a complex with Mcm16p and Mcm22p	Outer kinetochore protein that forms a complex with Mcm16p and Mcm22p; may bind the kinetochore to spindle microtubules; required for the spindle assembly checkpoint; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-I and fission yeast mis6
S000004374	YLR382C	NAM2	Nuclear Accommodation of Mitochondria	882067	884751	-1	Mitochondrial leucyl-tRNA synthetase	Mitochondrial leucyl-tRNA synthetase; also has direct role in splicing of several mitochondrial group I introns; indirectly required for mitochondrial genome maintenance; human homolog LARS2 can complement yeast null mutant, and is implicated in Perrault syndrome
S000004376	YLR384C	IKI3	Insensitive to KIller toxin	888851	892900	-1	Subunit of Elongator complex	Subunit of Elongator complex; Elongator is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD)
S000004377	YLR385C	SWC7	SWr Complex	892992	893390	-1	Protein of unknown function	Protein of unknown function; component of the Swr1p complex that incorporates Htz1p into chromatin
S000004378	YLR386W	VAC14	VACuole morphology and inheritance mutant	893628	896270	1	Enzyme regulator	Enzyme regulator; involved in synthesis of phosphatidylinositol 3,5-bisphosphate, in control of trafficking of some proteins to the vacuole lumen via the MVB, and in maintenance of vacuole size and acidity; binds negative (Fig4p) and positive (Fab1p) regulators of PtdIns(3,5)P(2) to control endolysosome function; similar to mammalian Vac14p
S000004379	YLR387C	REH1	REI1 Homolog	896374	897672	-1	Cytoplasmic 60S subunit biogenesis factor	Cytoplasmic 60S subunit biogenesis factor; associates with pre-60S particles; similar to Rei1p and shares partially redundant function in cytoplasmic 60S subunit maturation; contains dispersed C2H2 zinc finger domains
S000004380	YLR388W	RPS29A	Ribosomal Protein of the Small subunit	898651	898821	1	Protein component of the small (40S) ribosomal subunit	Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S29 and bacterial S14; RPS29A has a paralog, RPS29B, that arose from the whole genome duplication
S000004381	YLR389C	STE23	STErile	899577	902660	-1	Metalloprotease	Metalloprotease; involved in N-terminal processing of pro-a-factor to mature form; expressed in both haploids and diploids; one of two yeast homologs of human insulin-degrading enzyme (hIDE); homolog Axl1p is also involved in processing of pro-a-factor
S000004382	YLR390W	ECM19	ExtraCellular Mutant	903066	903404	1	Protein of unknown function	Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
S000004384	YLR392C	ART10	Arrestin-Related Trafficking adaptors	904749	906305	-1	Protein of unknown function that contains 2 PY motifs	Protein of unknown function that contains 2 PY motifs; ubiquinated by Rsp5p; overexpression confers resistance to arsenite; green fluorescent protein (GFP)-fusion protein localizes it to the cytoplasm; non-essential gene
S000004385	YLR393W	ATP10	ATP synthase	907079	907918	1	Assembly factor for the F0 sector of mitochondrial F1F0 ATP synthase	Assembly factor for the F0 sector of mitochondrial F1F0 ATP synthase; mitochondrial inner membrane protein; interacts genetically with ATP6
S000004386	YLR394W	CST9	Chromosome STability	907950	909398	1	SUMO E3 ligase	SUMO E3 ligase; required for synaptonemal complex formation; localizes to synapsis initiation sites on meiotic chromosomes; associates with centromeres early in meiosis, then with chromosome axes and finally with double-strand break sites that are engaged in repair by crossovers; potential Cdc28p substrate
S000004387	YLR395C	COX8	Cytochrome c OXidase	909729	909965	-1	Subunit VIII of cytochrome c oxidase (Complex IV)	Subunit VIII of cytochrome c oxidase (Complex IV); Complex IV is the terminal member of the mitochondrial inner membrane electron transport chain
S000004388	YLR396C	VPS33	Vacuolar Protein Sorting	910235	912310	-1	ATP-binding protein that is a subunit of the HOPS and CORVET complexes	ATP-binding protein that is a subunit of the HOPS and CORVET complexes; essential for protein sorting, vesicle docking, and fusion at the vacuole; binds to SNARE domains
S000004389	YLR397C	AFG2	ATPase Family Gene	912550	914892	-1	ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex	ATPase of the CDC48/PAS1/SEC18 (AAA) family, forms a hexameric complex; is essential for pre-60S maturation and release of several preribosome maturation factors; releases Rlp24p from purified pre-60S particles in vitro; target of the ribosomal biosynthesis inhibitor diazaborine; may be involved in degradation of aberrant mRNAs
S000004390	YLR398C	SKI2	SuperKIller	915156	919019	-1	Ski complex component and putative RNA helicase	Ski complex component and putative RNA helicase; mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay; mutations in the human ortholog, SKIV2L, causes Syndromic diarrhea/Trichohepatoenteric (SD/THE) syndrome
S000004391	YLR399C	BDF1	BromoDomain Factor	919536	921596	-1	BET family transcriptional regulator	BET family transcriptional regulator; associates with TFIID, and is involved in transcription initiation at TATA-containing promoters; essential for meiosis and expression of meiosis-specific genes;  binds acetylated histone H4 at double-strand break (DSB) sites, and promotes end resection, loading of RPA and Rad51p and ds break repair by homologous recombination; contains two bromodomains that correspond to the C-terminal region of mammalian TAF1; partially redundant with paralog Bdf2p
S000004392	YLR400W	""	""	922063	922536	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000004393	YLR401C	DUS3	DihydroUridine Synthase	922442	924448	-1	Dihydrouridine synthase	Dihydrouridine synthase; member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus4p; contains a consensus oleate response element (ORE) in its promoter region; forms nuclear foci upon DNA replication stress
S000004394	YLR402W	""	""	924889	925080	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000004395	YLR403W	SFP1	Split Finger Protein	925568	927619	1	Regulates transcription of ribosomal protein and biogenesis genes	Regulates transcription of ribosomal protein and biogenesis genes; regulates response to nutrients and stress, G2/M transitions during mitotic cell cycle and DNA-damage response, and modulates cell size; regulated by TORC1 and Mrs6p; sequence of zinc finger, ChIP localization data, and protein-binding microarray (PBM) data, and computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p at others; can form the [ISP+] prion
S000004396	YLR404W	SEI1	SEIpin	928743	929600	1	Seipin involved in lipid droplet (LD) assembly	Seipin involved in lipid droplet (LD) assembly; controls lipid particle morphology, number, and size; promotes initiation of LD formation on the ER; ensures that LDs bud from the ER towards the cytosolic side of the membrane; forms a complex with Ldb16p at ER-LD contact sites, stabilizing these sites; null mutants have localized accumulation of phosphatidic acid (PA) marker proteins; BSCL2, human homolog implicated in congenital lipodystrophy, complements yeast null mutant
S000004397	YLR405W	DUS4	DihydroUridine Synthase	929789	930892	1	Dihydrouridine synthase	Dihydrouridine synthase; member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus3p
S000004398	YLR406C	RPL31B	Ribosomal Protein of the Large subunit	931065	931755	-1	Ribosomal 60S subunit protein L31B	Ribosomal 60S subunit protein L31B; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31B has a paralog, RPL31A, that arose from the whole genome duplication
S000004399	YLR407W	""	""	932967	933653	1	Putative protein of unknown function	Putative protein of unknown function; null mutant displays elongated buds and a large fraction of budded cells have only one nucleus
S000004400	YLR408C	BLS1	BLoS1-homolog	933885	934253	-1	Subunit of the BLOC-1 complex involved in endosomal maturation	Subunit of the BLOC-1 complex involved in endosomal maturation; green fluorescent protein (GFP)-fusion protein localizes to the endosome; YLR408C is not an essential gene
S000004401	YLR409C	UTP21	U Three Protein	934414	937233	-1	Subunit of U3-containing 90S preribosome and SSU processome complexes	Subunit of U3-containing 90S preribosome and SSU processome complexes; involved in production of 18S rRNA and assembly of small ribosomal subunit; synthetic defect with STI1 Hsp90 cochaperone; human homolog linked to glaucoma; Small Subunit processome is also known as SSU processome
S000004402	YLR410W	VIP1	""	937541	940981	1	Bifunctional inositol pyrophosphate kinase and phosphatase	Bifunctional inositol pyrophosphate kinase and phosphatase; contains an N-terminal PP-InsP kinase domain that phosphorylates inositol hexakisphosphate and heptakisphosphate, and a C-terminal phosphatase domain that hydrolyzes both 1PP-InsP5 and 5PP-InsP5; IP7 levels decrease during starvation, suggesting a role for PP-InsP enzymes in Pi homeostasis; may regulate the dimorphic switch and the function of the cortical actin cytoskeleton
S000004404	YLR412W	BER1	Benomyl REsistant	948368	949192	1	Protein involved in microtubule-related processes	Protein involved in microtubule-related processes; GFP-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YLR412W is not an essential gene; similar to Arabidopsis SRR1 gene
S000004405	YLR413W	INA1	INdicator of Abscission	951156	953183	1	Protein of unknown function	Protein of unknown function; not an essential gene; YLR413W has a paralog, FAT3, that arose from the whole genome duplication
S000004406	YLR414C	PUN1	Plasma membrane protein Upregulated during Nitrogen stress	953353	954144	-1	Plasma membrane protein with a role in cell wall integrity	Plasma membrane protein with a role in cell wall integrity; co-localizes with Sur7p in punctate membrane patches; null mutant displays decreased thermotolerance; transcription induced upon cell wall damage and metal ion stress
S000004407	YLR415C	""	""	954259	954597	-1	Putative protein of unknown function	Putative protein of unknown function; YLR415C is not an essential gene
S000004408	YLR416C	""	""	954491	954889	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000004409	YLR417W	VPS36	Vacuolar Protein Sorting	955010	956710	1	Component of ESCRT-II complex	Component of ESCRT-II complex; contains GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into endosome; plays role in lager yeast flocculation under brewing conditions; plays role in formation of mutant huntingtin (Htt) aggregates in yeast
S000004410	YLR418C	CDC73	Cell Division Cycle	956914	958095	-1	Component of the Paf1p complex	Component of the Paf1p complex; binds to and modulates the activity of RNA polymerases I and II; required for expression of certain genes, modification of some histones, and telomere maintenance; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay; role in preventing L-A mycovirus pathogenesis; protein abundance increases in response to DNA replication stress; human homolog, parafibromin, is a tumour suppressor linked to breast, renal and gastric cancers
S000004411	YLR419W	""	""	958428	962735	1	Putative helicase with limited sequence similarity to human Rb protein	Putative helicase with limited sequence similarity to human Rb protein; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YLR419W is not an essential gene
S000004412	YLR420W	URA4	URAcil requiring	963785	964879	1	Dihydroorotase	Dihydroorotase; catalyzes the third enzymatic step in the de novo biosynthesis of pyrimidines, converting carbamoyl-L-aspartate into dihydroorotate
S000004413	YLR421C	RPN13	Regulatory Particle Non-ATPase	965090	965560	-1	Subunit of the 19S regulatory particle of the 26S proteasome lid	Subunit of the 19S regulatory particle of the 26S proteasome lid; acts as a ubiquitin receptor for the proteasome; null mutants accumulate ubiquitinated Gcn4p and display decreased 26S proteasome stability; protein abundance increases in response to DNA replication stress
S000004414	YLR422W	DCK1	DoCK1 homolog	965897	971695	1	Dock family protein (Dedicator Of CytoKinesis), homolog of human DOCK1	Dock family protein (Dedicator Of CytoKinesis), homolog of human DOCK1; upstream component for regulation through the small GTPase Rho5p; may form a complex with Lmo1p that acts as a GEF for Rho5p; interacts with Ino4p; cytoplasmic protein that relocates to mitochondria under oxidative stress; implicated in mitophagy; not an essential protein; DOCK proteins act as guanine nucleotide exchange factors
S000004415	YLR423C	ATG17	AuTophaGy related	971917	973170	-1	Scaffold protein responsible for phagophore assembly site organization	Scaffold protein responsible for phagophore assembly site organization; regulatory subunit of an autophagy-specific complex that includes Atg1p and Atg13p; stimulates Atg1p kinase activity; human ortholog RB1CC1/FIP200 interacts with p53, which inhibits autophagy in human cells
S000004416	YLR424W	SPP382	Suppressor of PrP38 #2	973395	975521	1	Essential protein that forms a dimer with Ntr2p	Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation
S000004417	YLR425W	TUS1	TOR Unique function Suppressor	982894	986817	1	Guanine nucleotide exchange factor (GEF) that modulates Rho1p activity	Guanine nucleotide exchange factor (GEF) that modulates Rho1p activity; involved in the cell integrity signaling pathway; interacts with Rgl1p; localization of Tus1p to the bed neck is regulated by Rgl1p; multicopy suppressor of tor2 mutation and ypk1 ypk2 double mutation; potential Cdc28p substrate
S000004418	YLR426W	TDA5	Topoisomerase I Damage Affected	987062	988113	1	Putative protein of unknown function	Putative protein of unknown function; detected in highly purified mitochondria in high-throughput studies; proposed to be involved in resistance to mechlorethamine and streptozotocin; null mutant sensitive to expression of top1-T722A allele
S000004419	YLR427W	MAG2	""	988428	990440	1	Cytoplasmic protein of unknown function	Cytoplasmic protein of unknown function; induced in response to mycotoxin patulin; ubiquitinated protein similar to the human ring finger motif protein RNF10; predicted to be involved in repair of alkylated DNA due to interaction with MAG1
S000004420	YLR428C	""	""	990617	990961	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF CRN1
S000004421	YLR429W	CRN1	CoRoNin	990777	992732	1	Coronin	Coronin; cortical actin cytoskeletal component that associates with the Arp2p/Arp3p complex to regulate its activity; plays a role in regulation of actin patch assembly
S000004422	YLR430W	SEN1	Splicing ENdonuclease	993434	1000129	1	ATP-dependent 5' to 3' RNA/DNA and DNA helicase	ATP-dependent 5' to 3' RNA/DNA and DNA helicase; subunit of the exosome-associated Nrd1p complex that mediates 3' end formation of snRNAs, snoRNAs, CUTs and some mRNAs; helicase-independent role in transcription-coupled repair; coordinates replication with transcription, associating with moving forks and preventing errors that occur when forks encounter transcribed regions; homolog of Senataxin, implicated in Ataxia-Oculomotor Apraxia 2 and a dominant form of juvenile ALS
S000004423	YLR431C	ATG23	AuTophaGy related	1000342	1001703	-1	Peripheral membrane protein required for autophagy and CVT	Peripheral membrane protein required for autophagy and CVT; required for cytoplasm-to-vacuole targeting (Cvt) pathway and efficient macroautophagy; cycles between the phagophore assembly site (PAS) and non-PAS locations; forms a complex with Atg9p and Atg27p
S000004424	YLR432W	IMD3	IMP Dehydrogenase	1002557	1004128	1	Inosine monophosphate dehydrogenase	Inosine monophosphate dehydrogenase; catalyzes the rate-limiting step in the de novo synthesis of GTP; member of a four-gene family in S. cerevisiae, constitutively expressed; IMD3 has a paralog, IMD4, that arose from the whole genome duplication
S000004425	YLR433C	CNA1	CalciNeurin A	1004347	1006008	-1	Calcineurin A	Calcineurin A; one isoform (the other is Cmp2p) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1; regulates the function of Aly1p alpha-arrestin; CNA1 has a paralog, CMP2, that arose from the whole genome duplication
S000004426	YLR434C	""	""	1006025	1006408	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF TSR2/YLR435W
S000004427	YLR435W	TSR2	Twenty S rRNA accumulation	1006378	1006995	1	Protein with a potential role in pre-rRNA processing	Protein with a potential role in pre-rRNA processing
S000004428	YLR436C	ECM30	ExtraCellular Mutant	1007421	1011245	-1	Protein of unknown function	Protein of unknown function; may play a role in cell wall biosynthesis, mutants have abormal relative levels of mannose and glucose and have Gap1p sorting and transport defects; (GFP)-fusion protein localizes to the cytoplasm
S000004429	YLR437C	DIF1	Damage-regulated Import Facilitator	1011622	1012023	-1	Protein that regulates nuclear import of Rnr2p and Rnr4p	Protein that regulates nuclear import of Rnr2p and Rnr4p; phosphorylated by Dun1p in response to DNA damage and degraded; N-terminal half shows similarity to S. pombe Spd1 protein; DIF1 has a paralog, SML1, that arose from the whole genome duplication
S000004430	YLR438W	CAR2	Catabolism of ARginine	1012501	1013775	1	L-ornithine transaminase (OTAse)	L-ornithine transaminase (OTAse); catalyzes the second step of arginine degradation, expression is dually-regulated by allophanate induction and a specific arginine induction process; not nitrogen catabolite repression sensitive; protein abundance increases in response to DNA replication stress; human homolog OAT complements yeast null mutant
S000004431	YLR439W	MRPL4	Mitochondrial Ribosomal Protein, Large subunit	1014491	1015450	1	Mitochondrial ribosomal protein of the large subunit	Mitochondrial ribosomal protein of the large subunit; homolog of prokaryotic L29 ribosomal protein; located at the ribosomal tunnel exit
S000004432	YLR440C	SEC39	SECretory	1015568	1017697	-1	Component of the Dsl1p tethering complex	Component of the Dsl1p tethering complex; this complex interacts with ER SNAREs Sec20p and Use1p; mediates Sey1p-independent homotypic ER fusion; proposed to be involved in protein secretion; localizes to the ER and nuclear envelope
S000004433	YLR441C	RPS1A	Ribosomal Protein of the Small subunit	1018141	1018908	-1	Ribosomal protein of the small (40S) subunit	Ribosomal protein of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1A has a paralog, RPS1B, that arose from the whole genome duplication
S000004434	YLR442C	SIR3	Silent Information Regulator	1019315	1022251	-1	Silencing protein	Silencing protein; interacts with Sir2p, Sir4p, and histone H3/H4 tails to establish transcriptionally silent chromatin; required for spreading of silenced chromatin; recruited to chromatin through interaction with Rap1p; C-terminus assumes variant winged helix-turn-helix (wH) fold that mediates homodimerization, which is critical for holo-SIR complex loading; required for telomere hypercluster formation in quiescent yeast cells; has paralog ORC1 from whole genome duplication
S000004435	YLR443W	ECM7	ExtraCellular Mutant	1022625	1023971	1	Putative integral membrane protein with a role in calcium uptake	Putative integral membrane protein with a role in calcium uptake; non-essential protein; mutant has cell wall defects and Ca+ uptake deficiencies; transcription is induced under conditions of zinc deficiency
S000004436	YLR444C	""	""	1023686	1023988	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000004437	YLR445W	GMC2	Grand Meiotic recombination Cluster	1024189	1024837	1	Protein involved in meiotic crossing over	Protein involved in meiotic crossing over; component of the Synaptonemal Complex (SC) along with Ecm11p; required for the efficient loading of the SC transverse filament protein, Zip1p; promotes SUMOylation of Ecm11p; mutants are delayed in meiotic nuclear division and are defective in synaptonemal complex assembly; transcription is regulated by Ume6p and induced in response to alpha factor; also detected in peroxisomes
S000004438	YLR446W	NGK1	N-acetylGlucosamine Kinase	1025214	1026515	1	N-acetylglucosamine kinase	N-acetylglucosamine kinase; GlcNAc kinase that requires a divalent cations and can utilize a broad range of NTPs as phosphoryl donor; phylogenically distinct from known GlcNAc kinases and hexokinases; transcript is upregulated during sporulation and the unfolded protein response; contains a hexokinase-like ATPase domain; NGK1 is not an essential gene
S000004439	YLR447C	VMA6	Vacuolar Membrane Atpase	1026856	1027893	-1	Subunit d of the V0 integral membrane domain of V-ATPase	Subunit d of the V0 integral membrane domain of V-ATPase; part of the electrogenic proton pump found in the endomembrane system; required for V1 domain assembly on the vacuolar membrane; the V0 integral membrane domain of vacuolar H+-ATPase (V-ATPase) has five subunits
S000004440	YLR448W	RPL6B	Ribosomal Protein of the Large subunit	1028854	1029768	1	Ribosomal 60S subunit protein L6B	Ribosomal 60S subunit protein L6B; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6B has a paralog, RPL6A, that arose from the whole genome duplication
S000004441	YLR449W	FPR4	FKBP Proline Rotamase (isomerase)	1030834	1032012	1	Peptidyl-prolyl cis-trans isomerase (PPIase)	Peptidyl-prolyl cis-trans isomerase (PPIase); nuclear proline isomerase; affects expression of multiple genes via its role in nucleosome assembly; catalyzes isomerization of proline residues in histones H3 and H4, which affects lysine methylation of those histones; PPIase domain acts as a transcriptional repressor when tethered to DNA by lexA, and repressor activity is dependent on PPIase activity; contains a nucleoplasmin-like fold and can form pentamers
S000004442	YLR450W	HMG2	3-Hydroxy-3-MethylGlutaryl-coenzyme a reductase	1032627	1035764	1	HMG-CoA reductase	HMG-CoA reductase; converts HMG-CoA to mevalonate, a rate-limiting step in sterol biosynthesis; one of two isozymes; overproduction induces assembly of peripheral ER membrane arrays and short nuclear-associated membrane stacks; forms foci at nuclear periphery upon DNA replication stress; primarily expressed on the peri-membrane side of the ER; human homolog HMGCR can complement yeast hmg2 mutant
S000004443	YLR451W	LEU3	LEUcine biosynthesis	1036093	1038753	1	Zinc-knuckle transcription factor, repressor and activator	Zinc-knuckle transcription factor, repressor and activator; regulates genes involved in branched chain amino acid biosynthesis and ammonia assimilation; acts as a repressor in leucine-replete conditions and as an activator in the presence of alpha-isopropylmalate, an intermediate in leucine biosynthesis that accumulates during leucine starvation
S000004444	YLR452C	SST2	SuperSensiTive	1039270	1041366	-1	GTPase-activating protein for Gpa1p	GTPase-activating protein for Gpa1p; regulates desensitization to alpha factor pheromone; also required to prevent receptor-independent signaling of the mating pathway; member of the RGS (regulator of G-protein signaling) family
S000004445	YLR453C	RIF2	Rap1p-Interacting Factor	1041799	1042986	-1	Protein that binds to the Rap1p C-terminus	Protein that binds to the Rap1p C-terminus; inhibits MRX-dependent Tel1p activity by acting directly on Rad50p and discharging its activated ATP-bound state, thereby rendering the MRX complex incompetent for Tel1p activation; acts synergistically with Rif1p to help control telomere length, establish telomeric silencing; deletion results in telomere elongation; RIF2 has paralog ORC4 that arose from the whole genome duplication
S000004446	YLR454W	FMP27	Found in Mitochondrial Proteome	1043998	1051884	1	Protein with structural similarity to lipid transport protein Vps13p	Protein with structural similarity to lipid transport protein Vps13p; localizes to multiple membrane contact sites between organelles including mitochondria–ER contact sites; non-tagged protein is detected in highly purified mitochondria in high-throughput studies
S000004447	YLR455W	PDP3	PWWP Domain-containing Protein	1053629	1054543	1	Component of the NuA3b histone acetyltransferase complex	Component of the NuA3b histone acetyltransferase complex; binds to Pol II and stimulates elongation; regulates interaction between NuA3b and H3K36me3 at transcribed regions of genes; contains PWWP domain; protein abundance increases in response to DNA replication stress; relocalizes to the cytosol in response to hypoxia; extensive sequence similarity (~25% identity; ~39% similarity) to the N-terminal region of human NDF
S000004448	YLR456W	""	""	1055070	1055684	1	Protein of unknown function	Protein of unknown function; predicted to encode a pyridoxal 5'-phosphate synthase based on sequence similarity but purified protein does not possess this activity, nor does it bind flavin mononucleotide (FMN); null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2; YLR456W has a paralog, YPR172W, that arose from the whole genome duplication
S000004449	YLR457C	NBP1	Nap1 Binding Protein	1055812	1056771	-1	Spindle pole body (SPB) component	Spindle pole body (SPB) component; required for the insertion of the duplication plaque into the nuclear membrane during SPB duplication; essential for bipolar spindle formation; component of the Mps2p-Bbp1p complex; NBP1 has a paralog, YPR174C, that arose from the whole genome duplication
S000004450	YLR458W	""	""	1056455	1056835	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of essential NBP1/YLR457C gene required for mitosis
S000004452	YLR460C	""	""	1059757	1060887	-1	Member of the quinone oxidoreductase family	Member of the quinone oxidoreductase family; up-regulated in response to the fungicide mancozeb; possibly up-regulated by iodine
S000004453	YLR461W	PAU4	seriPAUperin family	1062919	1063281	1	Member of the seripauperin multigene family	Member of the seripauperin multigene family; encoded mainly in subtelomeric regions; SWAT-GFP fusion protein localizes to the endoplasmic reticulum and vacuole, while mCherry fusion localizes to just the vacuole; active during alcoholic fermentation; regulated by anaerobiosis; negatively regulated by oxygen; repressed by heme
S000004460	YML001W	YPT7	Yeast Protein Two	267174	267800	1	Rab family GTPase	Rab family GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion; localizes to sites of contact between the vacuole and mitochondria (vCLAMPs); interacts with the cargo selection/retromer complex for retrograde sorting; similar to mammalian Rab7
S000004461	YML002W	VRL1	""	264541	266754	1	Putative protein of unknown function	Putative protein of unknown function; expression induced by heat and by calcium shortage; in many strains, YML002W is continuous with YML003W, and is predicted to encode a single protein of 1090 aa with a full-length VPS9 domain; in strains S288C, W303, and CEN.PK, a single thymine residue is deleted near the 3' end of YML003W at ChrXIII:264337, causing a frameshift and premature stop codon, truncating YML003W
S000004462	YML003W	""	""	263483	264355	1	Truncated 5' end of YML002W	Truncated 5' end of YML002W; in many strains, YML002W is continuous with YML003W, and is predicted to encode a single protein of 1090 aa with a full-length VPS9 domain; in strains S288C, W303, and CEN.PK, a single thymine residue is deleted near the 3' end of YML003W at ChrXIII:264337, causing a frameshift and premature stop codon, truncating YML003W
S000004463	YML004C	GLO1	GLyOxalase	261705	262685	-1	Monomeric glyoxalase I	Monomeric glyoxalase I; catalyzes the detoxification of methylglyoxal (a by-product of glycolysis) via condensation with glutathione to produce S-D-lactoylglutathione; required for full activity of O-acetyl homoserine sulfhydrylase, Met17p; expression regulated by methylglyoxal levels and osmotic stress
S000004464	YML005W	TRM12	TRna Methyltransferase	260221	261609	1	S-adenosylmethionine-dependent methyltransferase	S-adenosylmethionine-dependent methyltransferase; required for wybutosine formation in phenylalanine-accepting tRNA; member of the seven beta-strand family
S000004465	YML006C	GIS4	GIg1-2 Suppressor	256092	258416	-1	CAAX box containing protein of unknown function	CAAX box containing protein of unknown function; proposed to be involved in the RAS/cAMP signaling pathway
S000004466	YML007W	YAP1	Yeast AP-1	253848	255800	1	Basic leucine zipper (bZIP) transcription factor	Basic leucine zipper (bZIP) transcription factor; required for oxidative stress tolerance; activated by H2O2 through the multistep formation of disulfide bonds and transit from the cytoplasm to the nucleus; Yap1p is degraded in the nucleus after the oxidative stress has passed; mediates resistance to cadmium; relative distribution to the nucleus increases upon DNA replication stress; YAP1 has a paralog, CAD1, that arose from the whole genome duplication
S000004467	YML008C	ERG6	ERGosterol biosynthesis	251839	252990	-1	Delta(24)-sterol C-methyltransferase	Delta(24)-sterol C-methyltransferase; converts zymosterol to fecosterol in the ergosterol biosynthetic pathway by methylating position C-24; localized to lipid particles, the plasma membrane-associated endoplasmic reticulum, and the mitochondrial outer membrane
S000004468	YML009C	MRPL39	Mitochondrial Ribosomal Protein, Large subunit	251304	251516	-1	Mitochondrial ribosomal protein of the large subunit	Mitochondrial ribosomal protein of the large subunit
S000004469	YML009C-A	""	""	250675	251001	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORFs SPT5/YML010W and YML009W-B
S000004470	YML010W	SPT5	SuPpressor of Ty's	247677	250868	1	Spt4p/5p (DSIF) transcription elongation factor complex subunit	Spt4p/5p (DSIF) transcription elongation factor complex subunit; the Spt4/5 complex binds to ssRNA in a sequence-specific manner, and in concert with RNAP I and II has multiple roles regulating transcriptional elongation, RNA processing, quality control, and transcription-coupled repair; interacts with DNA upstream of RNAPII and the non-template strand of the transcription bubble; Spt5p is the only transcription elongation factor conserved in all domains of life
S000004471	YML009W-B	""	""	250435	250911	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion mutation confers an increase in Ty1 transposition
S000004472	YML011C	RAD33	RADiation sensitive	246895	247428	-1	Protein involved in nucleotide excision repair	Protein involved in nucleotide excision repair; green fluorescent protein (GFP)-fusion protein localizes to the nucleus
S000004473	YML012W	ERV25	ER Vesicle	246116	246751	1	Member of the p24 family involved in ER to Golgi transport	Member of the p24 family involved in ER to Golgi transport; role in misfolded protein quality control; forms a heterotrimeric complex with Erp1, Erp2p, and Emp24,
S000004474	YML012C-A	""	""	245537	245914	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1
S000004475	YML013W	UBX2	UBiquitin regulatory X	244149	245903	1	Bridging factor involved in ER-associated protein degradation (ERAD)	Bridging factor involved in ER-associated protein degradation (ERAD); bridges the cytosolic Cdc48p-Npl1p-Ufd1p ATPase complex and the membrane associated Ssm4p and Hrd1p ubiquitin ligase complexes; contains a UBX (ubiquitin regulatory X) domain and a ubiquitin-associated (UBA) domain; redistributes from the ER to lipid droplets during the diauxic shift and stationary phase; required for the maintenance of lipid homeostasis; required for mitochondrial protein translocation-associated degradation
S000004476	YML014W	TRM9	TRna Methyltransferase	243225	244064	1	tRNA methyltransferase	tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses
S000004477	YML015C	TAF11	TATA binding protein-Associated Factor	241989	243029	-1	TFIID subunit (40 kDa)	TFIID subunit (40 kDa); involved in RNA polymerase II transcription initiation, similar to histone H3 with atypical histone fold motif of Spt3-like transcription factors
S000004478	YML016C	PPZ1	Protein Phosphatase Z	239458	241536	-1	Serine/threonine protein phosphatase Z, isoform of Ppz2p	Serine/threonine protein phosphatase Z, isoform of Ppz2p; involved in regulation of potassium transport, which affects osmotic stability, cell cycle progression, and halotolerance
S000004479	YML017W	PSP2	Polymerase SuPpressor	236588	238731	1	Asn rich cytoplasmic protein with a role in clathrin suppresion	Asn rich cytoplasmic protein with a role in clathrin suppresion; contains RGG motifs; high-copy suppressor of group II intron-splicing defects of a mutation in MRS2 and of a conditional mutation in POL1 (DNA polymerase alpha); possible role in mitochondrial mRNA splicing
S000004480	YML018C	""	""	234771	235952	-1	Protein of unknown function	Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of the vacuole; physical interaction with Atg27p suggests a possible role in autophagy; YML018C is not an essential gene; relative distribution to the vacuolar membrane decreases upon DNA replication stress; YML018C has a paralog, THI74, that arose from the whole genome duplication
S000004481	YML019W	OST6	OligoSaccharylTransferase	233457	234455	1	Subunit of the oligosaccharyltransferase complex of the ER lumen	Subunit of the oligosaccharyltransferase complex of the ER lumen; complex catalyzes asparagine-linked glycosylation of newly synthesized proteins; similar to and partially functionally redundant with Ost3p
S000004482	YML020W	""	""	231149	233143	1	Putative protein of unknown function	Putative protein of unknown function
S000004483	YML021C	UNG1	Uracil DNA N-Glycosylase	229734	230813	-1	Uracil-DNA glycosylase	Uracil-DNA glycosylase; required for repair of uracil in DNA formed by spontaneous cytosine deamination; efficiently excises uracil from single-stranded DNA in vivo; not required for strand-specific mismatch repair; cell-cycle regulated, expressed in late G1; localizes to mitochondria and nucleus
S000004484	YML022W	APT1	Adenine PhosphoribosylTransferase	228937	229500	1	Adenine phosphoribosyltransferase	Adenine phosphoribosyltransferase; catalyzes the formation of AMP from adenine and 5-phosphoribosylpyrophosphate; involved in the salvage pathway of purine nucleotide biosynthesis; APT1 has a paralog, APT2, that arose from the whole genome duplication
S000004485	YML023C	NSE5	Non-SMC Element	226994	228664	-1	Component of the SMC5-SMC6 complex	Component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair
S000004486	YML024W	RPS17A	Ribosomal Protein of the Small subunit	225889	226697	1	Ribosomal protein 51 (rp51) of the small (40s) subunit	Ribosomal protein 51 (rp51) of the small (40s) subunit; homologous to mammalian ribosomal protein S17, no bacterial homolog; RPS17A has a paralog, RPS17B, that arose from the whole genome duplication
S000004487	YML025C	YML6	""	224406	225365	-1	Mitochondrial ribosomal protein of the large subunit	Mitochondrial ribosomal protein of the large subunit; has similarity to E. coli L4 ribosomal protein and human mitoribosomal MRP-L4 protein; essential for viability, unlike most other mitoribosomal proteins
S000004488	YML026C	RPS18B	Ribosomal Protein of the Small subunit	222987	223828	-1	Protein component of the small (40S) ribosomal subunit	Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S18 and bacterial S13; RPS18B has a paralog, RPS18A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
S000004489	YML027W	YOX1	Yeast homeobOX	221406	222563	1	Homeobox transcriptional repressor	Homeobox transcriptional repressor; binds to Mcm1p and to early cell cycle boxes (ECBs) in the promoters of cell cycle-regulated genes expressed in M/G1 phase; expression is cell cycle-regulated; phosphorylated by Cdc28p; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOX1 has a paralog, YHP1, that arose from the whole genome duplication
S000004490	YML028W	TSA1	Thiol-Specific Antioxidant	220138	220728	1	Thioredoxin peroxidase	Thioredoxin peroxidase; acts as both ribosome-associated and free cytoplasmic antioxidant; self-associates to form a HMW chaperone complex under oxidative stress; chaperone activity essential for growth in zinc deficiency; hydrogen peroxide receptor and signal transducer in a light sensing pathway; required for telomere length maintenance; binds and modulates Cdc19p activity; protein abundance increases and forms cytoplasmic foci during DNA replication stress
S000004491	YML029W	USA1	U1-Snp1 Associating	217362	219878	1	Scaffold subunit of the Hrd1p ubiquitin ligase	Scaffold subunit of the Hrd1p ubiquitin ligase; also promotes ligase oligomerization; involved in ER-associated protein degradation (ERAD); interacts with the U1 snRNP-specific protein, Snp1p
S000004492	YML030W	RCF1	Respiratory superComplex Factor	216435	216914	1	Cytochrome c oxidase subunit	Cytochrome c oxidase subunit; required for assembly of the Complex III-Complex IV supercomplex, and for assembly of Cox13p and Rcf2p into cytochrome c oxidase; similar to Rcf2p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; required for growth under hypoxic conditions; member of the hypoxia induced gene family; C. elegans and human orthologs are functional in yeast
S000004493	YML031W	NDC1	Nuclear Division Cycle	214189	216156	1	Subunit of the transmembrane ring of the nuclear pore complex (NPC)	Subunit of the transmembrane ring of the nuclear pore complex (NPC); contributes to nucleocytoplasmic transport, NPC biogenesis and spindle pole body duplication; homologous to human NDC1
S000004494	YML032C	RAD52	RADiation sensitive	212515	213930	-1	Protein involved in homologous recombination	Protein involved in homologous recombination; attenuates resection of DNA double-strand break ends and stimulates strand exchange by facilitating Rad51p binding to single-stranded DNA; anneals complementary single-stranded DNA; involved in the repair of double-strand breaks in DNA during vegetative growth and meiosis and UV induced sister chromatid recombination; phosphorylation by Cdc28p in G2/M phase promotes Rad52p ring interaction that leads to superstructure formation
S000004497	YML034W	SRC1	Spliced mRNA and Cell cycle regulated gene	209525	212155	1	Chromosome linkage inner nuclear membrane protein	Chromosome linkage inner nuclear membrane protein; CLIP, involved in perinuclear chromosome tethering and rDNA repeat stability; enriched at telomeres and subtelomeric regions; associates with and regulates subtelomeric genes, interacting with TREX (transcription export) factors; produces two splice variants with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant is aneuploidy tolerant; SRC1 has a paralog, HEH2, that arose from the whole genome duplication
S000004498	YML035C	AMD1	AMP Deaminase	206428	208860	-1	AMP deaminase	AMP deaminase; tetrameric enzyme that catalyzes the deamination of AMP to form IMP and ammonia; thought to be involved in regulation of intracellular purine (adenine, guanine, and inosine) nucleotide pools
S000004499	YML034C-A	""	""	209428	209826	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF SRC1/YML034W
S000004500	YML036W	CGI121	homolog of human CGI-121	205642	206293	1	Component of the EKC/KEOPS complex	Component of the EKC/KEOPS complex; EKC/KEOPS complex is required for t6A tRNA modification and telomeric TG1-3 recombination; may have role in transcription; Cgi121p is dispensable for tRNA modification; other complex members are Bud32p, Kae1p, Pcc1p, and Gon7p; ortholog of human TPRKB
S000004501	YML037C	""	""	204386	205408	-1	Putative protein of unknown function	Putative protein of unknown function; has some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene
S000004502	YML038C	YMD8	""	202775	204103	-1	Putative nucleotide sugar transporter	Putative nucleotide sugar transporter; has similarity to Vrg4p
S000004505	YML041C	VPS71	Vacuolar Protein Sorting	194913	195755	-1	Nucleosome-binding component of the SWR1 complex	Nucleosome-binding component of the SWR1 complex; SWR1 exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; required for vacuolar protein sorting
S000004506	YML042W	CAT2	Carnitine AcetylTransferase	192788	194800	1	Carnitine acetyl-CoA transferase	Carnitine acetyl-CoA transferase; present in both mitochondria and peroxisomes; transfers activated acetyl groups to carnitine to form acetylcarnitine which can be shuttled across membranes
S000004507	YML043C	RRN11	Regulation of RNA polymerase I	190244	191767	-1	Component of the core factor (CF) rDNA transcription factor complex	Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p
S000004509	YML046W	PRP39	Pre-mRNA Processing	181474	183363	1	U1 snRNP protein involved in splicing	U1 snRNP protein involved in splicing; contains multiple tetriatricopeptide repeats
S000004510	YML047C	PRM6	Pheromone-Regulated Membrane protein	180017	181075	-1	Potassium transporter that mediates K+ influx	Potassium transporter that mediates K+ influx; activates high-affinity Ca2+ influx system (HACS) during mating pheromone response; expression up-regulated in response to alpha factor; regulated by Ste12p during mating; localized to sites of polarized growth; member of a fungal-specific gene family; PRM6 has a paralog, KCH1, that arose from the whole genome duplication
S000004511	YML048W	GSF2	Glucose Signaling Factor	178426	179637	1	Endoplasmic reticulum (ER) localized integral membrane protein	Endoplasmic reticulum (ER) localized integral membrane protein; may promote secretion of certain hexose transporters, including Gal2p; involved in glucose-dependent repression
S000004512	YML047W-A	""	""	179893	180258	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF PRM6/YML047C
S000004513	YML049C	RSE1	RNA Splicing and ER-to-Golgi transport	174220	178305	-1	Protein involved in pre-mRNA splicing	Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport
S000004514	YML050W	AIM32	Altered Inheritance rate of Mitochondria	173139	174074	1	2Fe-2S mitochondrial protein involved in redox quality control	2Fe-2S mitochondrial protein involved in redox quality control; may maintain protein redox status by targeting oxidation sensitive cysteine residues; bishistidinyl coordinated, non-Rieske [2Fe-2S] cluster containing thiredoxin-like ferredoxin; forms a functional complex with Osm1p and Erv1p in the IMS; required for assembly of numerous mitochondrial import complexes; essential for anaerobiosis; localizes to the mitochondrial matrix and intermembrane space (IMS); homologous to APD1
S000004515	YML051W	GAL80	GALactose metabolism	171594	172901	1	Transcriptional regulator involved in the repression of GAL genes	Transcriptional regulator involved in the repression of GAL genes; involved in the repression of GAL genes in the absence of galactose; inhibits transcriptional activation by Gal4p; inhibition relieved by Gal3p or Gal1p binding
S000004516	YML052W	SUR7	SUppressor of Rvs167 mutation	170402	171310	1	Plasma membrane protein, component of eisosomes	Plasma membrane protein, component of eisosomes; long-lived protein that remains stable in eisosomes of mother cells while other eisosome proteins, Pil1p and Lsp1p, turn over; may function to anchor the eisosome in place; sporulation and plasma membrane sphingolipid content are altered in mutants; localizes to furrow-like invaginations (MCC patches)
S000004517	YML053C	""	""	169116	169754	-1	Putative protein of unknown function	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; overexpression causes a cell cycle delay or arrest; YML053C is not an essential gene
S000004518	YML054C	CYB2	CYtochrome B	165533	167308	-1	Cytochrome b2 (L-lactate cytochrome-c oxidoreductase)	Cytochrome b2 (L-lactate cytochrome-c oxidoreductase); component of the mitochondrial intermembrane space, required for lactate utilization; expression is repressed by glucose and anaerobic conditions
S000004519	YML055W	SPC2	Signal Peptidase Complex	164790	165326	1	Subunit of signal peptidase complex	Subunit of signal peptidase complex; complex catalyzes cleavage of N-terminal signal sequences of proteins targeted to the secretory pathway; inhibits SRP-independent translocation into the ER with STE24; homologous to mammalian SPC25; other members of the complex are Spc1p, Spc1p, and Sec11p
S000004520	YML056C	IMD4	IMP Dehydrogenase	162194	164176	-1	Inosine monophosphate dehydrogenase	Inosine monophosphate dehydrogenase; catalyzes the rate-limiting step in the de novo synthesis of GTP; member of a four-gene family in S. cerevisiae, constitutively expressed; IMD4 has a paralog, IMD3, that arose from the whole genome duplication
S000004521	YML057W	CMP2	CalModulin binding Protein	160180	161994	1	Calcineurin A	Calcineurin A; one isoform (the other is Cna1p) of the catalytic subunit of calcineurin, a Ca++/calmodulin-regulated protein phosphatase which regulates Crz1p (a stress-response transcription factor), the other calcineurin subunit is CNB1; regulates the function of Aly1p alpha-arrestin; CMP2 has a paralog, CNA1, that arose from the whole genome duplication
S000004522	YML057C-A	""	""	160023	160412	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene CMP2/YML057W
S000004523	YML058W	SML1	Suppressor of Mec1 Lethality	159383	159697	1	Ribonucleotide reductase inhibitor	Ribonucleotide reductase inhibitor; involved in regulating dNTP production; regulated by Mec1p and Rad53p during DNA damage and S phase; SML1 has a paralog, DIF1, that arose from the whole genome duplication
S000004524	YML059C	NTE1	Neuropathy Target Esterase	153219	158258	-1	Serine esterase	Serine esterase; homolog of human neuropathy target esterase (NTE); Nte1p-mediated phosphatidylcholine turnover influences transcription factor Opi1p localization, affecting transcriptional regulation of phospholipid biosynthesis genes
S000004525	YML060W	OGG1	8-OxoGuanine Glycosylase/lyase	151871	153001	1	Nuclear and mitochondrial glycosylase/lyase	Nuclear and mitochondrial glycosylase/lyase; specifically excises 7,8-dihydro-8-oxoguanine residues located opposite cytosine or thymine residues in DNA, repairs oxidative damage to mitochondrial DNA, contributes to UVA resistance
S000004526	YML061C	PIF1	Petite Integration Frequency	148953	151532	-1	DNA helicase, potent G-quadruplex DNA binder/unwinder	DNA helicase, potent G-quadruplex DNA binder/unwinder; possesses strand annealing activity; promotes DNA synthesis during break-induced replication; involved in crossover recombination; works with Hrq1p to maintain telomere length homeostasis; translation from different start sites produces mitochondrial (DNA repair and recombination) and nuclear (catalytic inhibitor of telomerase) isoforms; mutations affect Zn, Fe homeostasis; regulated by Rad53p-dependent phosphorylation in rho0 cells
S000004527	YML062C	MFT1	Mitochondrial Fusion Targeting	147505	148683	-1	Subunit of the THO complex	Subunit of the THO complex; THO is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance
S000004528	YML063W	RPS1B	Ribosomal Protein of the Small subunit	146482	147249	1	Ribosomal protein of the small (40S) subunit	Ribosomal protein of the small (40S) subunit; homologous to mammalian ribosomal protein S3A, no bacterial homolog; RPS1B has a paralog, RPS1A, that arose from the whole genome duplication
S000004529	YML064C	TEM1	TErmination of M phase	145139	145876	-1	GTPase involved in initiation of Mitotic Exit Network (MEN)	GTPase involved in initiation of Mitotic Exit Network (MEN); GTP-binding protein of the Ras superfamily; accumulates at daughter spindle pole body and activates MEN kinase cascade; controls actomyosin and septin dynamics during cytokinesis
S000004530	YML065W	ORC1	Origin Recognition Complex	142210	144954	1	Largest subunit of the origin recognition complex	Largest subunit of the origin recognition complex; involved in directing DNA replication by binding to replication origins; also involved in transcriptional silencing; exhibits ATPase activity; role in chromatin organization at replication origins, along with chromatin remodellers; ORC1 has a paralog, SIR3, that arose from the whole genome duplication
S000004531	YML066C	SMA2	Spore Membrane Assembly	140424	141533	-1	Meiosis-specific prospore membrane protein	Meiosis-specific prospore membrane protein; required to produce bending force necessary for proper assembly of the prospore membrane during sporulation
S000004532	YML067C	ERV41	ER Vesicle	139063	140214	-1	Protein localized to COPII-coated vesicles	Protein localized to COPII-coated vesicles; forms a complex with Erv46p; involved in the membrane fusion stage of transport; has homology to human ERGIC2 (PTX1) protein
S000004533	YML068W	ITT1	Inhibitor of Translation Termination	137550	138944	1	Protein that modulates the efficiency of translation termination	Protein that modulates the efficiency of translation termination; interacts with translation release factors eRF1 (Sup45p) and eRF3 (Sup35p) in vitro, contains a zinc finger domain characteristic of the TRIAD class of proteins
S000004534	YML069W	POB3	POl1 Binding	135500	137158	1	Subunit of the heterodimeric FACT complex (Spt16p-Pob3p)	Subunit of the heterodimeric FACT complex (Spt16p-Pob3p); FACT associates with chromatin via interaction with Nhp6Ap and Nhp6Bp, and reorganizes nucleosomes to facilitate access to DNA by RNA and DNA polymerases; protein abundance increases in response to DNA replication stress
S000004535	YML070W	DAK1	DihydroxyAcetone Kinase	133475	135229	1	Dihydroxyacetone kinase	Dihydroxyacetone kinase; required for detoxification of dihydroxyacetone (DHA); involved in stress adaptation
S000004536	YML071C	COG8	Conserved Oligomeric Golgi complex	129749	131572	-1	Component of the conserved oligomeric Golgi complex	Component of the conserved oligomeric Golgi complex; a cytosolic tethering complex (Cog1p through Cog8p) that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
S000004537	YML072C	TCB3	Three Calcium and lipid Binding domains (TriCalBin)	124730	129367	-1	Lipid-binding ER tricalbin involved in ER-plasma membrane tethering	Lipid-binding ER tricalbin involved in ER-plasma membrane tethering; one of at least 7 proteins (Ice2p, Ist2p, Scs2/Scs22p, Tcb1-Tcb3p) that affect cER tethering and contact with the plasma membrane; regulates PI4P levels by controlling access of Sac1p to its substrate PI4P in the PM; regulates PM phospholipid homeostasis; involved in intermembrane phospholipid transfer; also localizes to the mitochondria in a phosphorylated state and is enriched in bud membranes; mRNA is targeted to the bud tip
S000004539	YML074C	FPR3	Fk 506-sensitive Proline Rotamase	120089	121324	-1	Nucleolar peptidyl-prolyl cis-trans isomerase (PPIase)	Nucleolar peptidyl-prolyl cis-trans isomerase (PPIase); FK506 binding protein; affects expression of multiple genes via its role in nucleosome assembly; phosphorylated by casein kinase II (Cka1p-Cka2p-Ckb1p-Ckb2p) and dephosphorylated by Ptp1p; PPIase domain acts as a transcriptional repressor when tethered to DNA by lexA, and repressor activity is dependent on PPIase activity; FPR3 has a paralog, FPR4, that arose from the whole genome duplication
S000004540	YML075C	HMG1	3-Hydroxy-3-MethylGlutaryl-coenzyme a reductase	115734	118898	-1	HMG-CoA reductase	HMG-CoA reductase; catalyzes conversion of HMG-CoA to mevalonate, which is a rate-limiting step in sterol biosynthesis; one of two isozymes; localizes to nuclear envelope; overproduction induces formation of karmellae; forms foci at nuclear periphery upon DNA replication stress; expressed almost exclusively on the peri-nuclear side of the ER; human homolog HMGCR can complement yeast hmg1 mutant
S000004541	YML076C	WAR1	Weak Acid Resistance	112513	115347	-1	Homodimeric Zn2Cys6 zinc finger transcription factor	Homodimeric Zn2Cys6 zinc finger transcription factor; binds to a weak acid response element to induce transcription of PDR12 and FUN34, encoding an acid transporter and a putative ammonia transporter, respectively
S000004542	YML077W	BET5	Blocked Early in Transport	111865	112344	1	Core component of transport protein particle (TRAPP) complexes I-III	Core component of transport protein particle (TRAPP) complexes I-III; TRAPP complexes are related multimeric guanine nucleotide-exchange factors for the GTPase Ypt1p, regulating ER-Golgi traffic (TRAPPI), intra-Golgi traffic (TRAPPII), endosome-Golgi traffic (TRAPPII and III) and autophagy (TRAPPIII); human homology TRAPPC1 complements yeast null mutant
S000004543	YML078W	CPR3	Cyclosporin A-sensitive Proline Rotamase	111002	111550	1	Mitochondrial peptidyl-prolyl cis-trans isomerase (cyclophilin)	Mitochondrial peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; involved in protein refolding after import into mitochondria
S000004544	YML079W	CFF1	""	110247	110852	1	Protein required for production of 4-hydroxy-5-methylfuran-3(2H)-one (	Protein required for production of 4-hydroxy-5-methylfuran-3(2H)-one (MHF); cupin superfamily protein; has structural resemblance to plant storage and ligand binding proteins (canavalin, glycinin, auxin binding protein) and to some enzymes (epimerase, germin); localizes to nucleus and cytoplasm
S000004545	YML080W	DUS1	DihydroUridine Synthase	108806	110077	1	Dihydrouridine synthase	Dihydrouridine synthase; member of a widespread family of conserved proteins including Smm1p, Dus3p, and Dus4p; modifies pre-tRNA(Phe) at U17
S000004546	YML081W	TDA9	Topoisomerase I Damage Affected	104777	108532	1	Transcription factor that regulates acetate production	Transcription factor that regulates acetate production; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; TDA9 has a paralog, RSF2, that arose from the whole genome duplication
S000004547	YML082W	""	""	101862	103811	1	Putative protein predicted to have carbon-sulfur lyase activity	Putative protein predicted to have carbon-sulfur lyase activity; transcriptionally regulated by Upc2p via an upstream sterol response element; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and the cytoplasm; not an essential gene; YML082W has a paralog, STR2, that arose from the whole genome duplication
S000004548	YML083C	""	""	99794	101050	-1	Protein of unknown function	Protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; strong increase in transcript abundance during anaerobic growth compared to aerobic growth; cells deleted for YML083C do not exhibit growth defects in anerobic or anaerobic conditions
S000004549	YML084W	""	""	99489	99797	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000004551	YML086C	ALO1	D-Arabinono-1,4-Lactone Oxidase	95791	97371	-1	D-Arabinono-1,4-lactone oxidase	D-Arabinono-1,4-lactone oxidase; catalyzes the final step in biosynthesis of dehydro-D-arabinono-1,4-lactone, which is protective against oxidative stress; null mutant shows improved incorporation efficiency of noncanonical amino acids in place of amber stop codon
S000004552	YML087C	AIM33	Altered Inheritance rate of Mitochondria	94431	95369	-1	Protein of unknown function, highly conserved across species	Protein of unknown function, highly conserved across species; homolog of human CYB5R4; null mutant displays reduced frequency of mitochondrial genome loss; AIM33 has a paralog, PGA3, that arose from the whole genome duplication
S000004553	YML088W	UFO1	UV-F-box-HO	92235	94241	1	F-box receptor protein	F-box receptor protein; subunit of the Skp1-Cdc53-F-box receptor (SCF) E3 ubiquitin ligase complex; binds to phosphorylated Ho endonuclease, allowing its ubiquitination by SCF and subsequent degradation
S000004554	YML089C	""	""	91041	91409	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression induced by calcium shortage
S000004555	YML090W	""	""	90744	91130	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the dubious ORF YML089C; exhibits growth defect on a non-fermentable (respiratory) carbon source
S000004556	YML091C	RPM2	RNase P Mitochondrial	87123	90731	-1	Protein subunit of mitochondrial RNase P	Protein subunit of mitochondrial RNase P; has roles in nuclear transcription, cytoplasmic and mitochondrial RNA processing, and mitochondrial translation; distributed to mitochondria, cytoplasmic processing bodies, and the nucleus
S000004557	YML092C	PRE8	PRoteinase yscE	85987	86739	-1	Alpha 2 subunit of the 20S proteasome	Alpha 2 subunit of the 20S proteasome
S000004558	YML093W	UTP14	U Three Protein	83090	85789	1	Subunit of U3-containing Small Subunit (SSU) processome complex	Subunit of U3-containing Small Subunit (SSU) processome complex; involved in production of 18S rRNA and assembly of small ribosomal subunit
S000004559	YML094W	GIM5	Gene Involved in Microtubule biogenesis	82275	82849	1	Subunit of the heterohexameric cochaperone prefoldin complex	Subunit of the heterohexameric cochaperone prefoldin complex; prefoldin binds specifically to cytosolic chaperonin and transfers target proteins to it; prefoldin complex also localizes to chromatin of actively transcribed genes in the nucleus and facilitates transcriptional elongation
S000004560	YML095C	RAD10	RADiation sensitive	81481	82113	-1	Single-stranded DNA endonuclease (with Rad1p)	Single-stranded DNA endonuclease (with Rad1p); cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein
S000004561	YML094C-A	""	""	82219	82620	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene GIM5/YML094W; deletion confers sensitivity to GSAO
S000004562	YML096W	""	""	79909	81486	1	Putative protein with similarity to asparagine synthetases	Putative protein with similarity to asparagine synthetases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YML096W is not an essential gene and partially overlaps the verified gene RAD10
S000004563	YML097C	VPS9	Vacuolar Protein Sorting	78335	79690	-1	Guanine nucleotide exchange factor (GEF) and ubiquitin receptor	Guanine nucleotide exchange factor (GEF) and ubiquitin receptor; involved in vesicle-mediated vacuolar transport, including Golgi-endosome trafficking and sorting through the multivesicular body (MVB); stimulates the intrinsic guanine nucleotide exchange activity of Rab family members (Vps21p/Ypt52p/Ypt53p); partially redundant with GEF MUK1; required for localization of the CORVET complex to endosomes; similar to mammalian ras inhibitors; contains a Ub-interacting CUE domain
S000004564	YML098W	TAF13	TATA binding protein-Associated Factor	77267	77770	1	TFIID subunit (19 kDa)	TFIID subunit (19 kDa); involved in RNA polymerase II transcription initiation, similar to histone H4 with atypical histone fold motif of Spt3-like transcription factors
S000004565	YML099C	ARG81	ARGinine requiring	74398	77040	-1	Zinc finger transcription factor involved in arginine-responsive genes	Zinc finger transcription factor involved in arginine-responsive genes; Zn(2)-Cys(6) binuclear cluster domain type; involved in the regulation of arginine-responsive genes; acts with Arg80p and Arg82p
S000004566	YML100W	TSL1	Trehalose Synthase Long chain	70624	73920	1	Large subunit of trehalose 6-phosphate synthase/phosphatase complex	Large subunit of trehalose 6-phosphate synthase/phosphatase complex; Tps1p-Tps2p complex converts uridine-5'-diphosphoglucose and glucose 6-phosphate to trehalose; contributes to survival to acute lethal heat stress; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress; TSL1 has a paralog, TPS3, that arose from the whole genome duplication
S000004568	YML101C	CUE4	Coupling of Ubiquitin conjugation to ER degradation	69735	70088	-1	Protein of unknown function	Protein of unknown function; has a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; CUE4 has a paralog, CUE1, that arose from the whole genome duplication
S000004569	YML101C-A	""	""	69409	69726	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000004570	YML102W	CAC2	Chromatin Assembly Complex	68294	69700	1	Subunit of chromatin assembly factor I (CAF-1), with Rlf2p and Msi1p	Subunit of chromatin assembly factor I (CAF-1), with Rlf2p and Msi1p; chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure, deactivation of the DNA damage checkpoint after DNA repair, chromatin dynamics during transcription; and repression of divergent transcription; relocalizes to the cytosol in response to hypoxia
S000004571	YML103C	NUP188	NUclear Pore	62582	67549	-1	Subunit of the inner ring of the nuclear pore complex (NPC)	Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC organization and nucleocytoplasmic transport; homologous to human NUP188
S000004572	YML104C	MDM1	Mitochondrial Distribution and Morphology	58939	62322	-1	PtdIns-3-P binding protein that tethers the ER to vacuoles at NVJs	PtdIns-3-P binding protein that tethers the ER to vacuoles at NVJs; anchored in the ER membrane at nucleus-vacuole junctions and binds phosphatidylinositol 3-phosphate (PtdIns-3-P) in the vacuolar membrane via its Phox homology (PX) domain; expressed predominantly in late G1 to early S phase of the cell cycle; mutation affects nuclear and mitochondrial transmission to daughter buds; similar to 4 human genes, one of which (SNX14) is associated with neurological disease
S000004574	YML106W	URA5	URAcil requiring	56773	57453	1	Major orotate phosphoribosyltransferase (OPRTase) isozyme	Major orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA5 has a paralog, URA10, that arose from the whole genome duplication
S000004575	YML107C	PML39	Pre-mRNA Leakage	55265	56269	-1	Protein required for nuclear retention of unspliced pre-mRNAs	Protein required for nuclear retention of unspliced pre-mRNAs; required along with Mlp1p and Pml1p; anchored to the nuclear pore complex through an interaction between its bimodular nuclear basket-interaction domain (NuBaID) and Mlp1p and Mlp2p; localizes to the nuclear basket enables linkage between subpopulations of Mlp1p; associates with the subset of nuclear pores farthest from the nucleolus; homologous to human ZC3HC1, a NuBaID containing protein
S000004576	YML108W	""	""	54793	55110	1	Protein of unknown function	Protein of unknown function; structure defines a new subfamily of the split beta-alpha-beta sandwiches; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YML108W is not an essential gene; relative distribution to the nucleus increases upon DNA replication stress
S000004577	YML109W	ZDS2	Zillion Different Screens	51640	54468	1	Protein with a role in regulating Swe1p-dependent polarized growth	Protein with a role in regulating Swe1p-dependent polarized growth; involved in maintenance of Cdc55p in the cytoplasm where it promotes mitotic entry; interacts with silencing proteins at the telomere; implicated in the mitotic exit network through regulation of Cdc14p localization; ZDS2 has a paralog, ZDS1, that arose from the whole genome duplication
S000004578	YML110C	COQ5	COenzyme Q	50031	50954	-1	2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase	2-hexaprenyl-6-methoxy-1,4-benzoquinone methyltransferase; involved in ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with other ubiquinone biosynthetic enzymes; respiratory defect of the null mutant is partially complemented by human COQ5
S000004579	YML111W	BUL2	Binds Ubiquitin Ligase	46942	49704	1	Alpha-arrestin, component of the Rsp5p E3-ubiquitin ligase complex	Alpha-arrestin, component of the Rsp5p E3-ubiquitin ligase complex; ubiquitin-binding adaptor involved in intracellular amino acid permease sorting, functions in heat shock element mediated gene expression, essential for growth in stress conditions; BUL2 has a paralog, BUL1, that arose from the whole genome duplication
S000004580	YML112W	CTK3	Carboxy-Terminal domain Kinase	45063	45953	1	Gamma subunit of C-terminal domain kinase I	Gamma subunit of C-terminal domain kinase I; CTDK-I phosphorylates RNA polymerase II subunit Rpo21p to affect transcription and pre-mRNA 3' end processing, and also phosphorylates ribosomal protein Rps2p to increase translational fidelity; protein abundance increases in response to DNA replication stress
S000004581	YML113W	DAT1	DATin	44045	44791	1	DNA binding protein that recognizes oligo(dA).oligo(dT) tracts	DNA binding protein that recognizes oligo(dA).oligo(dT) tracts; Arg side chain in its N-terminal pentad Gly-Arg-Lys-Pro-Gly repeat is required for DNA-binding; relocalizes to the cytosol in response to hypoxia; not essential for viability
S000004582	YML114C	TAF8	TATA binding protein-Associated Factor	42043	43575	-1	TFIID subunit (65 kDa)	TFIID subunit (65 kDa); involved in RNA polymerase II transcription initiation
S000004583	YML115C	VAN1	VANadate resistance protein	40187	41794	-1	Component of the mannan polymerase I	Component of the mannan polymerase I; complex contains Van1p and Mnn9p and is involved in the first steps of mannan synthesis; mutants are vanadate-resistant
S000004584	YML116W	ATR1	AminoTriazole Resistance	38196	39824	1	Multidrug efflux pump of the major facilitator superfamily	Multidrug efflux pump of the major facilitator superfamily; required for resistance to aminotriazole and 4-nitroquinoline-N-oxide; ATR1 has a paralog, YMR279C, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
S000004585	YML117W	NAB6	Nucleic Acid Binding protein	34243	37647	1	mRNA-binding protein	mRNA-binding protein; binds to the 3'UTR of cell wall-related mRNAs and stabilizes these mRNAs, acting in parallel to cell wall integrity signaling to maintain expression of cell wall genes during stress; acts antagonistically to Nab6p; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP
S000004586	YML116W-A	""	""	37472	37774	1	Putative protein of unknown function	Putative protein of unknown function
S000004587	YML118W	NGL3	""	32334	33851	1	3'-5' exonuclease specific for poly-A RNAs	3'-5' exonuclease specific for poly-A RNAs; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; similar to Ngl1p; NGL3 has a paralog, NGL2, that arose from the whole genome duplication
S000004588	YML119W	""	""	30611	31684	1	Putative protein of unknown function	Putative protein of unknown function; YML119W is not an essential gene; potential Cdc28p substrate
S000004589	YML120C	NDI1	NADH Dehydrogenase Internal	28266	29807	-1	NADH:ubiquinone oxidoreductase	NADH:ubiquinone oxidoreductase; transfers electrons from NADH to ubiquinone in respiratory chain but does not pump protons, in contrast to higher eukaryotic multisubunit respiratory complex I; upon apoptotic stress, is activated in mitochondria by N-terminal cleavage, then translocates to cytoplasm to induce apoptosis; homolog of human AIFM2; yeast NDI1 complements several phenotypes of human cell line with mutated MT-ND4, implicated in Leber hereditary optic neuropathy
S000004590	YML121W	GTR1	GTp binding protein Resemblance	26930	27862	1	Subunit of TORC1-stimulating GTPase and EGO/GSE complex	Subunit of TORC1-stimulating GTPase and EGO/GSE complex; subunit of Gtr1-Gtr2 GTPase that activates TORC1 in response to amino acid stimulation; subunit of EGO/GSE vacuolar membrane complex that regulates exit from rapamycin-induced growth arrest and sorting of Gap1p; involved in phosphate transport, telomeric chromatin silencing and oxidative stress response; activated by Iml1p (GAP) subunit of SEACIT complex; targeted to vacuole via AP-3 pathway; similar to human RagA and RagB
S000004591	YML122C	""	""	26038	26418	-1	Putative protein of unknown function	Putative protein of unknown function; conserved among S. cerevisiae strains; YML122C is not an essential gene
S000004592	YML123C	PHO84	PHOsphate metabolism	24037	25800	-1	High-affinity inorganic phosphate (Pi) transporter	High-affinity inorganic phosphate (Pi) transporter; also low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity
S000004593	YML124C	TUB3	TUBulin	22048	23683	-1	Alpha-tubulin	Alpha-tubulin; associates with beta-tubulin (Tub2p) to form tubulin dimer, which polymerizes to form microtubules; expressed at lower level than Tub1p; TUB3 has a paralog, TUB1, that arose from the whole genome duplication
S000004594	YML125C	PGA3	Processing of Gas1p and ALP	20761	21699	-1	Putative cytochrome b5 reductase, localized to the plasma membrane	Putative cytochrome b5 reductase, localized to the plasma membrane; may be involved in regulation of lifespan; required for maturation of Gas1p and Pho8p, proposed to be involved in protein trafficking; PGA3 has a paralog, AIM33, that arose from the whole genome duplication
S000004595	YML126C	ERG13	ERGosterol biosynthesis	19060	20535	-1	3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase	3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase; catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis
S000004597	YML128C	MSC1	Meiotic Sister-Chromatid recombination	15135	16676	-1	Protein of unknown function	Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria and is phosphorylated
S000004598	YML129C	COX14	Cytochrome c OXidase	14541	14753	-1	Mitochondrial cytochrome c oxidase (complex IV) assembly factor	Mitochondrial cytochrome c oxidase (complex IV) assembly factor; also involved in translational regulation of Cox1p and prevention of Cox1p aggregation before assembly; associates with complex IV assembly intermediates and complex III/complex IV supercomplexes; located in the mitochondrial membrane
S000004600	YML131W	""	""	10198	11295	1	Protein of unknown function	Protein of unknown function; similar to medium chain dehydrogenase/reductases; expression induced by stresses including osmotic shock, DNA damaging agents, and other chemicals; GFP-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress
S000004603	YMR001C	CDC5	Cell Division Cycle	269019	271136	-1	Polo-like kinase required for mitotic exit	Polo-like kinase required for mitotic exit; regulates mitotic spindle assembly, nuclear shape, and protein localization to the nucleolus and SPBs; controls targeting and activation of Rho1p at the division site via Rho1p-GEFs; role in adaptation to DNA damage promotes genome instability during replicative senescence; regulates meiotic commitment, spindle assembly, chiasmata formation, recombination intermediate resolution and SC disassembly; human homologs PLK1 and PLK3 complement cdc5 mutants
S000004604	YMR002W	MIX17	Mitochondrial Intermembrane space CX(n)C motif protein	272193	272663	1	Mitochondrial intermembrane space protein	Mitochondrial intermembrane space protein; required for normal oxygen consumption; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress
S000004605	YMR003W	AIM34	Altered Inheritance rate of Mitochondria	273118	273714	1	Protein of unknown function	Protein of unknown function; GFP-fusion protein localizes to the mitochondria; null mutant is viable and displays reduced frequency of mitochondrial genome loss
S000004606	YMR004W	MVP1	Multi-copy suppressor of vps1	274017	275552	1	Protein required for sorting proteins to the vacuole	Protein required for sorting proteins to the vacuole; Mvp1p and Vps1p act in concert to promote membrane traffic to the vacuole; participates in transcription initiation and/or early elongation of specific genes; interacts with 'foot domain' of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions; protein abundance increases in response to DNA replication stress
S000004607	YMR005W	TAF4	TATA binding protein-Associated Factor	276045	277211	1	TFIID subunit (48 kDa)	TFIID subunit (48 kDa); involved in RNA polymerase II transcription initiation; potential Cdc28p substrate
S000004608	YMR006C	PLB2	PhosphoLipase B	277561	279681	-1	Phospholipase B (lysophospholipase) involved in lipid metabolism	Phospholipase B (lysophospholipase) involved in lipid metabolism; displays transacylase activity in vitro; overproduction confers resistance to lysophosphatidylcholine
S000004609	YMR007W	""	""	279960	280340	1	Putative protein of unknown function	Putative protein of unknown function; conserved among S. cerevisiae strains; YMR007W is not an essential gene
S000004610	YMR008C	PLB1	PhosphoLipase B	280590	282584	-1	Phospholipase B (lysophospholipase) involved in lipid metabolism	Phospholipase B (lysophospholipase) involved in lipid metabolism; required for efficient acyl chain remodeling of newly synthesized phosphatidylethanolamine-derived phosphatidylcholine; required for deacylation of phosphatidylcholine and phosphatidylethanolamine but not phosphatidylinositol; PLB1 has a paralog, PLB3, that arose from the whole genome duplication
S000004611	YMR009W	ADI1	Acireductone DIoxygenase	284102	284641	1	Acireductone dioxygenease involved in methionine salvage pathway	Acireductone dioxygenease involved in methionine salvage pathway; transcribed as polycistronic mRNA with YMR010W and regulated post-transcriptionally by RNase III (Rnt1p) cleavage; ADI1 mRNA is induced in heat shock conditions; human ortholog ADI1 can complement yeast adi1 mutant
S000004612	YMR010W	ANY1	Antagonizes Neo1 Yeast phospholipid flippase	285100	286317	1	Protein involved in phospholipid flippase function	Protein involved in phospholipid flippase function; null allele suppresses growth and membrane trafficking defects associated with all flippase null alleles; proposed function as a phospholipid scramblase that reduces membrane asymmetry; PQ loop family member; localizes to the endosome and trans-Golgi network; non-essential gene
S000004613	YMR011W	HXT2	HeXose Transporter	288079	289704	1	High-affinity glucose transporter of the major facilitator superfamily	High-affinity glucose transporter of the major facilitator superfamily; expression is induced by low levels of glucose and repressed by high levels of glucose
S000004614	YMR012W	CLU1	CLUstered mitochondria	291134	294967	1	Subunit of the eukaryotic translation initiation factor 3 (eIF3)	Subunit of the eukaryotic translation initiation factor 3 (eIF3); component of unknown function; deletion causes defects in mitochondrial organization but not in growth or translation initiation; can rescue cytokinesis and mitochondrial organization defects of the Dictyostelium cluA- mutant; eIF3 is also involved in programmed stop codon readthrough
S000004615	YMR013C	SEC59	SECretory	295179	296738	-1	Dolichol kinase	Dolichol kinase; catalyzes the terminal step in dolichyl monophosphate (Dol-P) biosynthesis; required for viability and for normal rates of lipid intermediate synthesis and protein N-glycosylation
S000004616	YMR014W	BUD22	BUD site selection	298868	300427	1	Protein required for rRNA maturation and ribosomal subunit biogenesis	Protein required for rRNA maturation and ribosomal subunit biogenesis; required for 18S rRNA maturation; also required for small ribosomal subunit biogenesis; cosediments with pre-ribosomal particles; mutation decreases efficiency of +1 Ty1 frameshifting and transposition, and affects budding pattern
S000004617	YMR015C	ERG5	ERGosterol biosynthesis	300869	302485	-1	C-22 sterol desaturase	C-22 sterol desaturase; a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs
S000004618	YMR016C	SOK2	Suppressor Of Kinase	303236	305593	-1	Nuclear protein that negatively regulates pseudohyphal differentiation	Nuclear protein that negatively regulates pseudohyphal differentiation; plays a regulatory role in the cyclic AMP (cAMP)-dependent protein kinase (PKA) signal transduction pathway; relocalizes to the cytosol in response to hypoxia; SOK2 has a paralog, PHD1, that arose from the whole genome duplication
S000004619	YMR017W	SPO20	SPOrulation	307489	308682	1	Meiosis-specific subunit of the t-SNARE complex	Meiosis-specific subunit of the t-SNARE complex; required for prospore membrane formation during sporulation; similar to but not functionally redundant with Sec9p; binds to phosphatidic acid; SNAP-25 homolog
S000004620	YMR018W	PEX9	PEroXin	310208	311752	1	Peroxisomal membrane signal receptor for peroxisomal matrix proteins	Peroxisomal membrane signal receptor for peroxisomal matrix proteins; oleate-inducible condition-specific import receptor for a subset of PTS1-containing matrix proteins; localizes to both the cytosol and the peroxisomal membrane; similar to human PEX5Rp, a peroxin protein 5 related protein; paralog of Pex5p
S000004621	YMR019W	STB4	Sin Three Binding protein	312156	315005	1	Putative transcription factor	Putative transcription factor; contains a Zn(II)2Cys6 zinc finger domain characteristic of DNA-binding proteins; computational analysis suggests a role in regulation of expression of genes encoding transporters; binds Sin3p in a two-hybrid assay;
S000004622	YMR020W	FMS1	Fenpropimorph-resistance Multicopy Suppressor	315377	316903	1	Polyamine oxidase	Polyamine oxidase; converts spermine to spermidine, which is required for the essential hypusination modification of translation factor eIF-5A; also involved in pantothenic acid biosynthesis
S000004623	YMR021C	MAC1	Metal binding ACtivator	317165	318418	-1	Copper-sensing transcription factor	Copper-sensing transcription factor; involved in regulation of genes required for high affinity copper transport; required for regulation of yeast copper genes in response to DNA-damaging agents; undergoes changes in redox state in response to changing levels of copper or MMS
S000004624	YMR022W	UBC7	UBiquitin-Conjugating enzyme	318680	319177	1	Ubiquitin conjugating enzyme	Ubiquitin conjugating enzyme; involved in the ER-associated protein degradation (ERAD) pathway and in the inner nuclear membrane-associated degradation (INMAD) pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly
S000004625	YMR023C	MSS1	Mitochondrial Splicing System	319437	321017	-1	Mitochondrial protein	Mitochondrial protein; forms a heterodimer complex with Mto1p that performs the 5-carboxymethylaminomethyl modification of the wobble uridine base in mitochondrial tRNAs; similar to human GTPBP3
S000004626	YMR024W	MRPL3	Mitochondrial Ribosomal Protein, Large subunit	321875	323047	1	Mitochondrial ribosomal protein of the large subunit	Mitochondrial ribosomal protein of the large subunit; located in close proximity to the polypeptide exit channel of the ribosome; mutations in human homolog MRPL44 cause childhood cardiomyopathy; human MRPL44 deficiency results in inefficient assembly of the mitochondrial ribosome, and in tissue-specific respiratory chain deficiency, manifesting as either Complex I+Complex IV or Complex IV deficiency, depending on a cell type
S000004627	YMR025W	CSI1	Cop9 Signalosome Interactor	323300	324187	1	Subunit of the Cop9 signalosome	Subunit of the Cop9 signalosome; which is required for deneddylation, or removal of the ubiquitin-like protein Rub1p from Cdc53p (cullin); involved in adaptation to pheromone signaling; functional equivalent of canonical Csn6 subunit of the COP9 signalosome
S000004628	YMR026C	PEX12	PEroXin	324236	325435	-1	C3HC4-type RING-finger peroxin and E3 ubiquitin ligase	C3HC4-type RING-finger peroxin and E3 ubiquitin ligase; required for peroxisome biogenesis and peroxisomal matrix protein import; forms translocation subcomplex with Pex2p and Pex10p; mutations in human homolog cause peroxisomal disorder
S000004629	YMR027W	""	""	325877	327289	1	A metal-dependent phosphatase, part of the DUF89 protein family	A metal-dependent phosphatase, part of the DUF89 protein family; dephosphorylates fructose-1-phosphate; human ortholog, C6orf211 is involved in response to DNA damage; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR027W is not an essential gene
S000004630	YMR028W	TAP42	Two A phosphatase Associated Protein	327482	328582	1	Essential protein involved in the TOR signaling pathway	Essential protein involved in the TOR signaling pathway; physically associates with the protein phosphatase 2A and the SIT4 protein phosphatase catalytic subunits
S000004631	YMR029C	FAR8	Factor ARrest	328660	330231	-1	Protein involved in recovery from arrest in response to pheromone	Protein involved in recovery from arrest in response to pheromone; acts in a cell cycle arrest recovery pathway independent from Far1p; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p
S000004632	YMR030W	RSF1	ReSpiration Factor	330793	331923	1	Protein required for respiratory growth	Protein required for respiratory growth; localized to both the nucleus and mitochondrion; may interact with transcription factors to mediate the transition to respiratory growth and activate transcription of nuclear and mitochondrial genes
S000004633	YMR031C	EIS1	EISosome	332212	334743	-1	Component of the eisosome required for proper eisosome assembly	Component of the eisosome required for proper eisosome assembly; similar to Uso1p; authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies; protein increases in abundance and relocalizes from plasma membrane to cytoplasm upon DNA replication stress; EIS1 has a paralog, YKL050C, that arose from the whole genome duplication
S000004634	YMR031W-A	""	""	334709	335035	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C
S000004635	YMR032W	HOF1	Homolog Of cdc Fifteen	335298	337307	1	F-BAR protein that regulates actin cytoskeleton organization	F-BAR protein that regulates actin cytoskeleton organization; binds and bundles actin filaments, linking them to septins; required for cytokinesis, actin cable organization, and secretory vesicle trafficking; regulates actomyosin ring dynamics and septin localization; N-term. half controls cell size and actin cable levels, while the C-term. half controls actin cable organization, inhibiting Bnr1p-mediated actin nucleation; forms axial striations/pillars at the bud neck; phosphorylated by Dbf2p
S000004637	YMR034C	RCH1	Regulator of Calcium Homeostasis	339418	340722	-1	Negative regulator of cytosolic calcium homeostasis	Negative regulator of cytosolic calcium homeostasis; localizes to the plasma membrane, concentrating at the bud neck during cell division; regulated by Crz1p through a CDRE promoter element; non-essential gene; putative transporter with sequence similarity to human SLC10A7; functional homolog of C. albicans RCH1
S000004638	YMR035W	IMP2	Inner Membrane Protease	341142	341675	1	Catalytic subunit of mitochondrial inner membrane peptidase complex	Catalytic subunit of mitochondrial inner membrane peptidase complex; required for maturation of mitochondrial proteins of the intermembrane space; complex contains two catalytic subunits (Imp1p and Imp2p that differ in substrate specificity), and Som1p
S000004639	YMR036C	MIH1	Mitotic Inducer Homolog	341856	343520	-1	Protein tyrosine phosphatase	Protein tyrosine phosphatase; involved in cell cycle control and retrograde protein transport; regulates the phosphorylation state of Cdc28p; modulates phosphorylation of retromer component Vps26p; homolog of S. pombe cdc25
S000004640	YMR037C	MSN2	Multicopy suppressor of SNF1 mutation	344403	346517	-1	Stress-responsive transcriptional activator	Stress-responsive transcriptional activator; activated in stochastic pulses of nuclear localization in response to various stress conditions; binds DNA at stress response elements of responsive genes; light sensing pathway component that accumulates in the nucleus in response to blue light; relative distribution to nucleus increases upon DNA replication stress
S000004641	YMR038C	CCS1	Copper Chaperone for SOD1	347511	348260	-1	Copper chaperone for superoxide dismutase Sod1p	Copper chaperone for superoxide dismutase Sod1p; involved in oxidative stress protection; Met-X-Cys-X2-Cys motif within N-terminus is involved in insertion of copper into Sod1p under conditions of copper deprivation; required for regulation of yeast copper genes in response to DNA-damaging agents; protein abundance increases in response to DNA replication stress; human homolog CCS can complement yeast ccs1 null mutant
S000004642	YMR039C	SUB1	SUppressor of TFIIB mutations	348644	349522	-1	Transcriptional regulator	Transcriptional regulator; facilitates elongation through factors that modify RNAP II; role in peroxide resistance involving Rad2p; role in nonhomologous end-joining (NHEJ) of ds breaks in plasmid DNA, but not chromosomal DNA; role in the hyperosmotic stress response through polymerase recruitment at RNAP II and RNAP III genes; negatively regulates sporulation; protein abundance increases in response to DNA replication stress; functionally complemented by human SUB1 (PC4)
S000004643	YMR040W	YET2	Yeast Endoplasmic reticulum Transmembrane protein	350381	350863	1	Protein of unknown function that may interact with ribosomes	Protein of unknown function that may interact with ribosomes; based on co-purification experiments; homolog of human BAP31 protein; YET2 has a paralog, YET1, that arose from the whole genome duplication
S000004644	YMR041C	ARA2	ARAbinose	350966	351973	-1	NAD-dependent arabinose dehydrogenase	NAD-dependent arabinose dehydrogenase; involved in biosynthesis of dehydro-D-arabinono-1,4-lactone; similar to plant L-galactose dehydrogenase
S000004645	YMR042W	ARG80	ARGinine requiring	352603	353136	1	Transcription factor involved in regulating arginine-responsive genes	Transcription factor involved in regulating arginine-responsive genes; acts with Arg81p and Arg82p
S000004646	YMR043W	MCM1	MiniChromosome Maintenance	353871	354731	1	Transcription factor	Transcription factor; involved in cell-type-specific transcription and pheromone response; plays a central role in the formation of both repressor and activator complexes; relocalizes to the cytosol in response to hypoxia
S000004647	YMR044W	IOC4	Iswi One Complex	355384	356811	1	Member of a complex (Isw1b) with Isw1p and Ioc2p	Member of a complex (Isw1b) with Isw1p and Ioc2p; interacts directly with H3K36me3 nucleosomes through its PWWP domain to recruit the Isw1b complex to open reading frames in a Set2p-dependent manner; Isw1b exhibits nucleosome-stimulated ATPase activity and acts within coding regions to coordinate transcription elongation with termination and processing
S000004650	YMR047C	NUP116	NUclear Pore	363364	366705	-1	FG-nucleoporin component of central core of the nuclear pore complex	FG-nucleoporin component of central core of the nuclear pore complex; contributes directly to nucleocytoplasmic transport and maintenance of the nuclear pore complex (NPC) permeability barrier; forms a stable association with Nup82p, Gle2p and two other FG-nucleoporins (Nsp1p and Nup159p); NUP116 has a paralog, NUP100, that arose from the whole genome duplication
S000004651	YMR048W	CSM3	Chromosome Segregation in Meiosis	366981	367934	1	Replication fork associated factor	Replication fork associated factor; required for stable replication fork pausing; component of the DNA replication checkpoint pathway; required for accurate chromosome segregation during meiosis; forms nuclear foci upon DNA replication stress
S000004652	YMR049C	ERB1	Eukaryotic Ribosome Biogenesis	368094	370517	-1	Constituent of 66S pre-ribosomal particles	Constituent of 66S pre-ribosomal particles; forms a complex with Nop7p and Ytm1p that is required for maturation of the large ribosomal subunit; required for maturation of the 25S and 5.8S ribosomal RNAs; binds RNA via its C-terminal domain; homologous to mammalian Bop1
S000004655	YMR052C-A	""	""	380069	380434	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000004656	YMR052W	FAR3	Factor ARrest	379586	380200	1	Protein of unknown function	Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress
S000004657	YMR053C	STB2	Sin Three Binding protein	380346	382898	-1	Protein that interacts with Sin3p in a two-hybrid assay	Protein that interacts with Sin3p in a two-hybrid assay; part of a large protein complex with Sin3p and Stb1p; STB2 has a paralog, STB6, that arose from the whole genome duplication
S000004658	YMR054W	STV1	Similar To VPH1	383303	385975	1	Subunit a of vacuolar-ATPase V0 domain	Subunit a of vacuolar-ATPase V0 domain; encodes one of two isoforms, located in both Golgi and endosomal V-ATPase complexes while VPH1 encodes second isoform and is located in vacuolar V-ATPase complexes; interaction with PI(4)P is required for efficient localization and function of Stv1-containing V-ATPases; targeted to vacuole via AP-3 pathway
S000004659	YMR055C	BUB2	Budding Uninhibited by Benzimidazole	386101	387021	-1	Mitotic exit network regulator	Mitotic exit network regulator; forms GTPase-activating Bfa1p-Bub2p complex that binds Tem1p and spindle pole bodies, blocks cell cycle progression before anaphase in response to spindle and kinetochore damage
S000004660	YMR056C	AAC1	ADP/ATP Carrier	387315	388244	-1	Mitochondrial inner membrane ADP/ATP translocator	Mitochondrial inner membrane ADP/ATP translocator; exchanges cytosolic ADP for mitochondrially synthesized ATP; phosphorylated; Aac1p is a minor isoform while Pet9p is the major ADP/ATP translocator; relocalizes from mitochondrion to cytoplasm upon DNA replication stress
S000004661	YMR057C	""	""	388359	388730	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified ORF AAC1
S000004662	YMR058W	FET3	FErrous Transport	388822	390732	1	Ferro-O2-oxidoreductase	Ferro-O2-oxidoreductase; multicopper oxidase that oxidizes ferrous (Fe2+) to ferric iron (Fe3+) for subsequent cellular uptake by transmembrane permease Ftr1p; required for high-affinity iron uptake and involved in mediating resistance to copper ion toxicity, belongs to class of integral membrane multicopper oxidases; protein abundance increases in response to DNA replication stress
S000004663	YMR059W	SEN15	Splicing ENdonuclease	391099	391485	1	Subunit of the tRNA splicing endonuclease	Subunit of the tRNA splicing endonuclease; tRNA splicing endonuclease (Sen complex) is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen complex also cleaves the CBP1 mRNA at the mitochondrial surface
S000004664	YMR060C	SAM37	Sorting and Assembly Machinery	391532	392515	-1	Component of the Sorting and Assembly Machinery (SAM) complex	Component of the Sorting and Assembly Machinery (SAM) complex; the SAM (or TOB) complex is located in the mitochondrial outer membrane; binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; contributes to SAM complex stability
S000004665	YMR061W	RNA14	poly(A) mRNA metabolism	392755	394788	1	Component of the cleavage and polyadenylation factor I (CF I)	Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping and maintenance of genome stability; relocalizes to the cytosol in response to hypoxia
S000004666	YMR062C	ARG7	ARGinine requiring	395054	396379	-1	Mitochondrial ornithine acetyltransferase	Mitochondrial ornithine acetyltransferase; catalyzes the fifth step in arginine biosynthesis; also possesses acetylglutamate synthase activity, regenerates acetylglutamate while forming ornithine
S000004667	YMR063W	RIM9	Regulator of IME2	397077	397796	1	Plasma membrane protein of unknown function	Plasma membrane protein of unknown function; involved in the proteolytic activation of Rim101p in response to alkaline pH; interacts with Rim21p and Dfg16p to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; has similarity to A. nidulans PalI;
S000004668	YMR064W	AEP1	ATPase ExPression	397903	399459	1	Protein required for expression of the mitochondrial OLI1 gene	Protein required for expression of the mitochondrial OLI1 gene; mitochondrial OLI1 gene encodes subunit 9 of F1-F0 ATP synthase
S000004669	YMR065W	KAR5	KARyogamy	399702	401216	1	Protein required for nuclear membrane fusion during karyogamy	Protein required for nuclear membrane fusion during karyogamy; localizes to the membrane with a soluble portion in the endoplasmic reticulum lumen, may form a complex with Jem1p and Kar2p; similar to zebrafish Brambleberry protein; expression of the gene is regulated by pheromone
S000004670	YMR066W	SOV1	Synthesis Of Var	401541	404237	1	Mitochondrial protein of unknown function	Mitochondrial protein of unknown function
S000004671	YMR067C	UBX4	UBiquitin regulatory X	404323	405573	-1	UBX domain-containing protein that interacts with Cdc48p	UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the role of the Cdc48p-Nplp-Ufd1p AAA ATPase complex in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress; human ortholog ASPSCR1 partially complements the yeast null mutant 
S000004672	YMR068W	AVO2	Adheres VOraciously (to TOR2)	406304	407584	1	Subunit of TORC2, a regulator of plasma membrane (PM) homeostasis	Subunit of TORC2, a regulator of plasma membrane (PM) homeostasis; TORC2 also regulates actin cytoskeletal dynamics during polarized growth and cell wall integrity; interacts with Slm1p and Slm2p, homologous PH domain-containing TORC2 substrates; PM retention is dependent on Avo3p
S000004673	YMR069W	NAT4	N-AcetylTransferase	407709	408566	1	N alpha-acetyl-transferase	N alpha-acetyl-transferase; involved in acetylation of the N-terminal residues of histones H4 and H2A
S000004674	YMR070W	MOT3	Modifier of Transcription	409154	410626	1	Transcriptional repressor, activator	Transcriptional repressor, activator; role in cellular adjustment to osmotic stress including modulation of mating efficiency; involved in repression of subset of hypoxic genes by Rox1p, repression of several DAN/TIR genes during aerobic growth, ergosterol biosynthetic genes in response to hyperosmotic stress; contributes to recruitment of Tup1p-Cyc8p general repressor to promoters; relocalizes to cytosol under hypoxia; forms [MOT3+] prion under anaerobic conditions
S000004675	YMR071C	TVP18	Tlg2-Vesicle Protein	410762	411265	-1	Integral membrane protein	Integral membrane protein; localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments; may have a role in intracellular sterol transport
S000004676	YMR072W	ABF2	ARS-Binding Factor	411569	412120	1	Mitochondrial DNA-binding protein	Mitochondrial DNA-binding protein; involved in mitochondrial DNA replication and recombination, member of HMG1 DNA-binding protein family; activity may be regulated by protein kinase A phosphorylation; ABF2 has a paralog, IXR1, that arose from the whole genome duplication; human homolog TFAM can complement yeast abf2 mutant, rescuing the loss-of-mitochondrial DNA phenotype
S000004677	YMR073C	IRC21	Increased Recombination Centers	412268	412873	-1	Protein of unknown function	Protein of unknown function; may be involved in resistance to carboplatin and cisplatin; null mutant displays increase in spontaneous Rad52p foci; contains a lipid-binding domain and binds cardiolipin in a large-scale study
S000004678	YMR074C	SDD2	Suppressor of Degenerative Death	413036	413473	-1	Protein with homology to human PDCD5	Protein with homology to human PDCD5; PDCD5 is involved in programmed cell death; N-terminal region forms a conserved triple-helix bundle structure; overproduction suppresses lethality due to expression of the dominant PET9 allele AAC2-A128P; overexpression promotes H2O2-induced apoptosis; YMR074C is not an essential gene; protein abundance increases in response to DNA replication stress
S000004679	YMR075C-A	""	""	415688	416053	-1	Protein of unknown function	Protein of unknown function; almost completely overlaps verified ORF RCO1/YMR075W
S000004680	YMR075W	RCO1	""	413982	416036	1	Essential component of the Rpd3S histone deacetylase complex	Essential component of the Rpd3S histone deacetylase complex; interacts with Eaf3p
S000004681	YMR076C	PDS5	Precocious Dissociation of Sisters	416196	420029	-1	Cohesion maintenance factor	Cohesion maintenance factor; involved in sister chromatid condensation and cohesion; colocalizes with cohesin on chromosomes; performs its cohesin maintenance function in pre-anaphase cells by protecting the integrity of the cohesion complex; regulates homolog pairing and facilitates synaptonemal complex formation, axis formation, interhomolog recombination and synapsis during meiosis; relocalizes to the cytosol in response to hypoxia; scaffolding protein contains 26 HEAT repeats  
S000004682	YMR077C	VPS20	Vacuolar Protein Sorting	421484	422149	-1	Myristoylated subunit of the ESCRT-III complex	Myristoylated subunit of the ESCRT-III complex; the endosomal sorting complex required for transport of transmembrane proteins into the multivesicular body pathway to the lysosomal/vacuolar lumen; cytoplasmic protein recruited to endosomal membranes
S000004683	YMR078C	CTF18	Chromosome Transmission Fidelity	422503	424728	-1	Subunit of a complex with Ctf8p	Subunit of a complex with Ctf8p; shares some subunits with Replication Factor C and is required for sister chromatid cohesion; may have overlapping functions with Rad24p in the DNA damage replication checkpoint
S000004684	YMR079W	SEC14	SECretory	424989	426059	1	Phosphatidylinositol/phosphatidylcholine transfer protein	Phosphatidylinositol/phosphatidylcholine transfer protein; involved in regulating PtdIns, PtdCho, and ceramide metabolism, products of which regulate intracellular transport and UPR; has a role in localization of lipid raft proteins; functionally homologous to mammalian PITPs; SEC14 has a paralog, YKL091C, that arose from the whole genome duplication
S000004685	YMR080C	NAM7	Nuclear Accommodation of Mitochondria	426712	429627	-1	ATP-dependent RNA helicase of the SFI superfamily	ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; binds to the small ribosomal subunit via an interaction with Rps26; forms cytoplasmic foci upon DNA replication stress
S000004686	YMR081C	ISF1	Increasing Suppression Factor	430079	431095	-1	Serine-rich, hydrophilic protein	Serine-rich, hydrophilic protein; overexpression suppresses growth defects of hap2, hap3, and hap4 mutants; expression is under glucose control; cotranscribed with NAM7 in a cyp1 mutant; ISF1 has a paralog, MBR1, that arose from the whole genome duplication
S000004687	YMR082C	""	""	431769	432125	-1	Putative protein of unknown function	Putative protein of unknown function; conserved among S. cerevisiae strains; YMR082C is not an essential gene
S000004688	YMR083W	ADH3	Alcohol DeHydrogenase	434788	435915	1	Mitochondrial alcohol dehydrogenase isozyme III	Mitochondrial alcohol dehydrogenase isozyme III; involved in the shuttling of mitochondrial NADH to the cytosol under anaerobic conditions and ethanol production
S000004689	YMR084W	""	""	436628	437416	1	Putative protein of unknown function	Putative protein of unknown function; YMR084W and adjacent ORF YMR085W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1
S000004690	YMR085W	""	""	437491	438789	1	Putative protein of unknown function	Putative protein of unknown function; YMR085W and adjacent ORF YMR084W are merged in related strains, and together are paralogous to glutamine-fructose-6-phosphate amidotransferase GFA1
S000004691	YMR086C-A	""	""	442026	442364	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000004692	YMR086W	SEG1	Stability of Eisosomes Guaranteed	439208	442090	1	Component of eisosome required for proper eisosome assembly	Component of eisosome required for proper eisosome assembly; precedes Pil1p/Lsp1p during eisosome formation, controls eisosome length and shape; diffusely distributed, forms heterogeneous patches at plasma membrane in small buds, also found in medium and large buds; expression repressed by cAMP; similar to A. gossypii SEG gene and to S. pombe Sle1p, important for generating eisosomes; SEG1 has a paralog, SEG2, that arose from the whole genome duplication
S000004693	YMR087W	PDL32	Protein of Dual Localization of 32 kDa	442527	443381	1	Putative ADP-ribose-1''-monophosphatase	Putative ADP-ribose-1''-monophosphatase; converts ADP-ribose-1''-monophosphate to ADP-ribose; may have a role in tRNA splicing; contains an A1pp domain
S000004694	YMR088C	VBA1	Vacuolar Basic Amino acid transporter	443414	445102	-1	Permease of basic amino acids in vacuolar membrane	Permease of basic amino acids in vacuolar membrane; targeted to vacuole via AP-3 pathway
S000004695	YMR089C	YTA12	Yeast Tat-binding Analog	445609	448086	-1	Mitochondrial inner membrane m-AAA protease component	Mitochondrial inner membrane m-AAA protease component; mediates degradation of misfolded or unassembled proteins; also required for correct assembly of mitochondrial enzyme complexes; overexpression of human AFG3L2 complements respiratory defect of yeast afg3 yta12 double null mutation, but overexpression of disease-associated AFG3L2 variants does not; expression of both human SPG7 (paraplegin) and AFG3L2 complements yeast yta12 afg3 double mutation
S000004696	YMR090W	""	""	449245	449928	1	Putative protein of unknown function	Putative protein of unknown function; similar to DTDP-glucose 4,6-dehydratases; GFP-fusion protein localizes to the cytoplasm; up-regulated in response to the fungicide mancozeb; not essential for viability
S000004697	YMR091C	NPL6	Nuclear Protein Localization	450058	451365	-1	Component of the RSC chromatin remodeling complex	Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Ldb7p, and Htl1p to form a module important for a broad range of RSC functions
S000004698	YMR092C	AIP1	Actin Interacting Protein	451632	453479	-1	Actin cortical patch component	Actin cortical patch component; interacts with the actin depolymerizing factor cofilin; inhibits elongation of aged ADP-actin filaments decorated with cofilin to maintain a high level of assembly-competent actin species; required to restrict cofilin localization to cortical patches; putative regulator of cytokinesis; contains WD repeats; mutations in human homolog WDR1 are associated with cardiac hypertrophy
S000004699	YMR093W	UTP15	U Three Protein	454015	455556	1	Nucleolar protein	Nucleolar protein; component of the small subunit (SSU) processome containing the U3 snoRNA that is involved in processing of pre-18S rRNA
S000004700	YMR094W	CTF13	Chromosome Transmission Fidelity	455825	457261	1	Subunit of the CBF3 complex	Subunit of the CBF3 complex; F-box protein of the leucine-rich-repeat family; required for assembly of the complex; CBF3 binds to the CDE III element of centromeres, bending the DNA upon binding, and may be involved in sister chromatid cohesion during mitosis
S000004701	YMR095C	SNO1	SNZ proximal Open reading frame	457285	457959	-1	Protein of unconfirmed function	Protein of unconfirmed function; involved in pyridoxine metabolism; expression is induced during stationary phase; forms putative glutamine amidotransferase complex with Snz1p, with Sno1p serving as the glutaminase; sno1-Δ affects abundance of mRNA from downstream gene CTF13
S000004702	YMR096W	SNZ1	SNooZe	458408	459301	1	Subunit of a two-component pyridoxal-5'-phosphate synthase	Subunit of a two-component pyridoxal-5'-phosphate synthase; involved in pyridoxine (vitamin B6) biosynthesis from glyceraldehyde-3-phosphate, ribose-5-phosphate and ammonia liberated from glutamine by glutaminase; forms a complex with Sno1p that functions as an active glutaminase; member of a stationary phase-induced gene family where transcriptional induction occurs during the postdiauxic phase; co-regulated with SNO1; paralog of SNZ2 and SNZ3
S000004703	YMR097C	MTG1	MiTochondrial GTPase	459424	460527	-1	Putative GTPase peripheral to the mitochondrial inner membrane	Putative GTPase peripheral to the mitochondrial inner membrane; essential for respiratory competence, likely functions in assembly of the large ribosomal subunit, has homologs in plants and animals
S000004704	YMR098C	ATP25	ATPase	460771	462609	-1	Protein that associates with mitochondrial ribosome	Protein that associates with mitochondrial ribosome; required for the stability of Oli1p (Atp9p) mRNA; also required for the Oli1p ring formation; YMR098C is not an essential gene
S000004705	YMR099C	""	""	463934	464827	-1	Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase)	Glucose-6-phosphate 1-epimerase (hexose-6-phosphate mutarotase); likely involved in carbohydrate metabolism; GFP-fusion protein localizes to both the nucleus and cytoplasm and is induced in response to the DNA-damaging agent MMS
S000004706	YMR100W	MUB1	MUlti Budding	466300	468162	1	MYND domain-containing protein	MYND domain-containing protein; component of the Mub1p-Ubr2p-Rad6p ubiquitin ligase complex, required for ubiquitination and degradation of Rpn4p; interacts with Ubr2p (E3) and indirectly with Rad6p (E2); short-lived protein degraded in a Ubr2p/Rad6p dependent manner; proposed to function as both a partner and substrate of the Ubr2p/Rad6p ubiquitin ligase; similar to the A. nidulans samB gene
S000004707	YMR101C	SRT1	Suppressor of Rer-Two	468445	469476	-1	Forms the dehydrodolichyl diphosphate synthase (DDS) complex with NUS1	Forms the dehydrodolichyl diphosphate synthase (DDS) complex with NUS1; involved in synthesis of long-chain dolichols (19-22 isoprene units; as opposed to Rer2p which synthesizes shorter-chain dolichols); localizes to lipid bodies; transcription is induced during stationary phase
S000004708	YMR102C	LAF1	Lam2/Ltc4-Associated Factor	469848	472352	-1	Sterol-binding beta-propeller protein	Sterol-binding beta-propeller protein; physically associates with sterol-binding StARkin domain-containing proteins Ysp2/Lam2/Ltc4 and Lam4/Ltc3; essential for retrograde transport of ergosterol from the plasma membrane to the endoplasmic reticulum at ER-PM contact sites; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; non-essential gene; has a paralog, DGR2, that arose from the whole genome duplication
S000004709	YMR103C	""	""	472902	473264	-1	Putative protein of unknown function	Putative protein of unknown function; conserved among S. cerevisiae strains; YMR103C is not an essential gene
S000004710	YMR104C	YPK2	Yeast Protein Kinase	473420	475453	-1	AGC-type S/T protein kinase	AGC-type S/T protein kinase; participates in the cell wall integrity signaling pathway; involved with Ypk1p in the TORC-dependent phosphorylation of ribosomal proteins Rps6a/b (S6), and in the direct phosphorylation of the Myo5p motor domain TEDS site; phosphorylated and activated by Pkh1/2p in the presence of phytosphingosine; direct phosphorylation by Tor2p regulates actin polarization; genetically redundant with YPK1; human homolog SGK2 can complement the inviability of a ypk1 ypk2 mutant
S000004711	YMR105C	PGM2	PhosphoGlucoMutase	475897	477606	-1	Phosphoglucomutase	Phosphoglucomutase; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism; functions as the acceptor for a Glc-phosphotransferase; protein abundance increases in response to DNA replication stress; PGM2 has a paralog, PGM1, that arose from the whole genome duplication
S000004712	YMR106C	YKU80	Yeast KU protein	478301	480190	-1	Subunit of telomeric Ku complex (Yku70p-Yku80p)	Subunit of telomeric Ku complex (Yku70p-Yku80p); involved in telomere length maintenance, structure and telomere position effect; required for localization of telomerase ribonucleoprotein via interaction with TLC1 guide RNA; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair; colocalizes with quiescent cell telomere hyperclusters
S000004713	YMR107W	SPG4	Stationary Phase Gene	483014	483361	1	Protein required for high temperature survival during stationary phase	Protein required for high temperature survival during stationary phase; not required for growth on nonfermentable carbon sources
S000004715	YMR109W	MYO5	MYOsin	486587	490246	1	One of two type I myosin motors	One of two type I myosin motors; involved in clathrin-mediated endocytosis; activation by TEDS site phosphorylation increases actin gliding, motor activity and ligand-induced endocytosis; contains proline-rich tail homology 2 (TH2) and SH3 domains; MYO5 deletion has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization; MYO5 has a paralog, MYO3, that arose from the whole genome duplication
S000004716	YMR110C	HFD1	Homolog of Fatty aldehyde Dehydrogenase	490394	491992	-1	Dehydrogenase involved in ubiquinone and sphingolipid metabolism	Dehydrogenase involved in ubiquinone and sphingolipid metabolism; converts 4-hydroxybenzaldehyde into 4-hydroxybenzoate for ubiquinone anabolism, hexadecenal to hexadecenoic acid in sphingosine 1-phosphate catabolism; human homolog ALDH3A2, mutated in Sjogren-Larsson syndrome, can rescue yeast hfd1 mutant; human ALDH3A1, but not ALDH3A2, rescues pABA- respiratory growth phenotype of hfd1 null; data suggest that dual functions of Hfd1p have diverged in human ALDH3A1, ALDH3A2
S000004717	YMR111C	EUC1	Enriches Ubiquitin on Chromatin	492404	493792	-1	Sequence-specific DNA binding protein	Sequence-specific DNA binding protein; binds to a sequence motif associated with distinct genomic hotspots of non-H2B protein ubiquitylation (ub-hotspots); interacts with Slx5p to recruit the Slx5-Slx8  SUMO-targeted Ub ligase (STUbL) complex to ub-hotspots; deletion prevents ub-hotspot formation and exacerbates heat sensitivity of cells deficient in Rpd3L histone deacetylase complex members; forms nuclear foci upon DNA replication stress
S000004719	YMR113W	FOL3	FOLic acid synthesis	494999	496282	1	Dihydrofolate synthetase, involved in folic acid biosynthesis	Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis; FOL3 has a paralog, RMA1, that arose from the whole genome duplication
S000004720	YMR114C	""	""	496343	497449	-1	Protein of unknown function	Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and cytoplasm; YMR114C is not an essential gene
S000004721	YMR115W	MGR3	Mitochondrial Genome Required	497704	499209	1	Subunit of the mitochondrial (mt) i-AAA protease supercomplex	Subunit of the mitochondrial (mt) i-AAA protease supercomplex; i-AAA degrades misfolded mitochondrial proteins; forms a subcomplex with Mgr1p that binds to substrates to facilitate proteolysis; required for growth of cells lacking mtDNA
S000004722	YMR116C	ASC1	Absence of growth Suppressor of Cyp1	499456	500688	-1	G-protein beta subunit and guanine dissociation inhibitor for Gpa2p	G-protein beta subunit and guanine dissociation inhibitor for Gpa2p; ortholog of RACK1 that inhibits translation; core component of the small (40S) ribosomal subunit; required to prevent frameshifting at ribosomes stalled at repeated CGA codons; regulates P-body formation induced by replication stress; represses Gcn4p in the absence of amino acid starvation; controls phosphorylation of multiple proteins
S000004723	YMR117C	SPC24	Spindle Pole Component	501250	501891	-1	Component of the kinetochore-associated Ndc80 complex	Component of the kinetochore-associated Ndc80 complex; involved in chromosome segregation, spindle checkpoint activity, and kinetochore clustering; evolutionarily conserved; other members include Ndc80p, Nuf2p, Spc24p, and Spc25p
S000004724	YMR118C	SHH3	SDH3 Homolog	502144	502734	-1	Putative mitochondrial inner membrane protein of unknown function	Putative mitochondrial inner membrane protein of unknown function; although similar to paralogous Sdh3p, Shh3p is not a stoichiometric subunit of either succinate dehydrogenase or of the TIM22 translocase; SHH3 has a paralog, SDH3, that arose from the whole genome duplication
S000004725	YMR119W	ASI1	Amino acid Sensor-Independent	505333	507207	1	Subunit of the inner nuclear membrane Asi ubiquitin ligase complex	Subunit of the inner nuclear membrane Asi ubiquitin ligase complex; the Asi complex targets both misfolded proteins of the inner nuclear membrane-associated degradation (INMAD) pathway and regulators of sterol biosynthesis for ubiquitin-mediated degradation; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signaling by targeting latent unprocessed forms of Stp1p and Stp2p, maintaining the repressed state of gene expression in the absence of inducing amino acids
S000004726	YMR119W-A	""	""	506996	507370	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 3' end of ORF ASI1/YMR119W
S000004727	YMR120C	ADE17	ADEnine	507502	509280	-1	Enzyme of 'de novo' purine biosynthesis	Enzyme of 'de novo' purine biosynthesis; contains both 5-aminoimidazole-4-carboxamide ribonucleotide transformylase and inosine monophosphate cyclohydrolase activities; ADE17 has a paralog, ADE16, that arose from the whole genome duplication; ade16 ade17 mutants require adenine and histidine
S000004728	YMR121C	RPL15B	Ribosomal Protein of the Large subunit	509734	510348	-1	Ribosomal 60S subunit protein L15B	Ribosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress
S000004729	YMR122C	""	""	510701	511075	-1	Putative protein of unknown function	Putative protein of unknown function; conserved among S. cerevisiae strains; YMR122C is not an essential gene
S000004730	YMR123W	PKR1	Pichia farinosa Killer toxin Resistance	513593	513961	1	V-ATPase assembly factor	V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress
S000004731	YMR124W	EPO1	Endoplasmic reticulum POlarization	514456	517287	1	Protein involved in septin-ER tethering	Protein involved in septin-ER tethering; interacts with ER membrane protein, Scs2p, and Shs1p, a septin ring component, at bud neck to create ER diffusion barrier; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W has a paralog, YLR031W, that arose from the whole genome duplication
S000004732	YMR125W	STO1	Suppressor of TOp1	517539	520446	1	Large subunit of the nuclear mRNA cap-binding protein complex	Large subunit of the nuclear mRNA cap-binding protein complex; interacts with Npl3p to carry nuclear poly(A)+ mRNA to cytoplasm; also involved in nuclear mRNA degradation and telomere maintenance; orthologous to mammalian CBP80
S000004733	YMR126C	DLT1	Defect at Low Temperature	520761	521789	-1	Protein of unknown function	Protein of unknown function; mutant sensitive to 6-azauracil (6AU) and mycophenolic acid (MPA)
S000004734	YMR127C	SAS2	Something About Silencing	522329	523345	-1	Histone acetyltransferase (HAT) catalytic subunit of the SAS complex	Histone acetyltransferase (HAT) catalytic subunit of the SAS complex; acetylates free histones and nucleosomes and regulates transcriptional silencing; member of the MYSTacetyltransferase family; other members are Sas4p and Sas5p
S000004735	YMR128W	ECM16	ExtraCellular Mutant	523696	527499	1	Essential DEAH-box ATP-dependent RNA helicase specific to U3 snoRNP	Essential DEAH-box ATP-dependent RNA helicase specific to U3 snoRNP; functions with methyltransferase Bud23p; predominantly nucleolar in distribution; required for 18S rRNA synthesis
S000004736	YMR129W	POM152	POre Membrane	527804	531817	1	Glycoprotein subunit of transmembrane ring of nuclear pore complex	Glycoprotein subunit of transmembrane ring of nuclear pore complex; contributes to nucleocytoplasmic transport, nuclear pore complex (NPC) biogenesis and spindle pole body duplication; type II transmembrane protein composed of N-terminal cytosolic and C-terminal nuclear envelope lumenal domains; homologous to human NUP210
S000004737	YMR130W	DPI35	Delta-Psi dependent mitochondrial Import protein of 35 kDa	532119	533027	1	Putative mitochondrial protein of unknown function	Putative mitochondrial protein of unknown function; DPI35 is not an essential gene
S000004738	YMR131C	RRB1	Regulator of Ribosome Biogenesis	533163	534698	-1	Specific assembly chaperone for ribosomal protein Rpl3p	Specific assembly chaperone for ribosomal protein Rpl3p; co-translationally associates with nascent Rpl3p, preventing aggregation, and facilitating nuclear import; required for biogenesis of preribosomal particles; essential gene; contains five WD-repeat motifs
S000004739	YMR132C	JLP2	dnaJ-Like Protein	534944	535570	-1	Protein of unknown function	Protein of unknown function; contains sequence that closely resembles a J domain (typified by the E. coli DnaJ protein)
S000004740	YMR133W	REC114	RECombination	536207	537609	1	Protein involved in early stages of meiotic recombination	Protein involved in early stages of meiotic recombination; possibly involved in the coordination of recombination and meiotic division; mutations lead to premature initiation of the first meiotic division
S000004741	YMR134W	ERG29	ERGosterol biosynthesis	537838	538551	1	Protein of unknown function involved in ergosterol biosynthesis	Protein of unknown function involved in ergosterol biosynthesis; conditional mutants produce less ergosterol, display impaired oxygen consumption, respiratory growth, mitochondrial iron utilization, and are more sensitive to oxidative stress; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; protein localizes to the cytoplasm, ER and nuclear envelope; highly conserved in ascomycetes
S000004742	YMR135C	GID8	Glucose Induced Degradation deficient	538689	540056	-1	Subunit of GID Complex, binds strongly to central component Vid30p	Subunit of GID Complex, binds strongly to central component Vid30p; GID Complex is involved in proteasome-dependent catabolite inactivation of fructose-1,6-bisphosphatase; recruits Rmd5p, Fyv10 and Vid28p to GID Complex; contains LisH, CTLH, and CRA domains that mediate binding to Vid30p (LisH) and Rmd5p and Vid28p (CTLH and CRA); dosage-dependent regulator of START
S000004743	YMR135W-A	""	""	539911	540444	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000004744	YMR136W	GAT2	""	541199	542881	1	Protein containing GATA family zinc finger motifs	Protein containing GATA family zinc finger motifs; similar to Gln3p and Dal80p; expression repressed by leucine
S000004745	YMR137C	PSO2	PSOralen derivative sensitive	542978	544963	-1	Nuclease required for DNA single- and double-strand break repair	Nuclease required for DNA single- and double-strand break repair; acts at a post-incision step in repair of breaks that result from interstrand cross-links produced by a variety of mono- and bi-functional psoralen derivatives; induced by UV-irradiation; forms nuclear foci upon DNA replication stress; translocates to mitochondria under genotoxic agents
S000004746	YMR138W	CIN4	Chromosome INstability	545155	545730	1	GTP-binding protein involved in beta-tubulin (Tub2p) folding	GTP-binding protein involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; regulated by the GTPase-activating protein, Cin2p, the human retinitis pigmentosa 2 (RP2) homolog
S000004747	YMR139W	RIM11	Regulator of IME2	546125	547237	1	Protein kinase	Protein kinase; required for signal transduction during entry into meiosis; promotes the formation of the Ime1p-Ume6p complex by phosphorylating Ime1p and Ume6p; shares similarity with mammalian glycogen synthase kinase 3-beta; protein abundance increases in response to DNA replication stress; RIM11 has a paralog, MRK1, that arose from the whole genome duplication
S000004748	YMR140W	SIP5	Snf1 Interacting Protein	547714	549183	1	Protein of unknown function	Protein of unknown function; interacts with both the Reg1p/Glc7p phosphatase and the Snf1p kinase; forms cytoplasmic foci upon DNA replication stress
S000004749	YMR141C	""	""	549736	550044	-1	Putative protein of unknown function	Putative protein of unknown function; conserved among S. cerevisiae strains; YMR141C is not an essential gene
S000004750	YMR142C	RPL13B	Ribosomal Protein of the Large subunit	550206	551207	-1	Ribosomal 60S subunit protein L13B	Ribosomal 60S subunit protein L13B; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13B has a paralog, RPL13A, that arose from the whole genome duplication
S000004751	YMR143W	RPS16A	Ribosomal Protein of the Small subunit	551928	552903	1	Protein component of the small (40S) ribosomal subunit	Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16A has a paralog, RPS16B, that arose from the whole genome duplication
S000004752	YMR144W	FDO1	Forkhead one interacting protein involved in DOnor preference	553362	554390	1	Protein involved in directionality of mating type switching	Protein involved in directionality of mating type switching; acts with Fkh1p to control which donor mating-type locus is inserted into MAT locus during mating type switching; localized to the nucleus; not an essential gene
S000004753	YMR145C	NDE1	NADH Dehydrogenase, External	554793	556475	-1	Mitochondrial external NADH dehydrogenase	Mitochondrial external NADH dehydrogenase; type II NAD(P)H:quinone oxidoreductase that catalyzes the oxidation of cytosolic NADH; Nde1p and Nde2p provide cytosolic NADH to the mitochondrial respiratory chain; NDE1 has a paralog, NDE2, that arose from the whole genome duplication
S000004754	YMR146C	TIF34	Translation Initiation Factor	557481	558524	-1	eIF3i subunit of the eukaryotic translation initiation factor 3 (eIF3)	eIF3i subunit of the eukaryotic translation initiation factor 3 (eIF3); subunit of the core complex of eIF3; essential for translation; stimulates rate of ribosomal scanning during translation reinitiation; eIF3 is also involved in programmed stop codon readthrough
S000004755	YMR147W	LDO45	Lipid Droplet Organization protein of 45 Kda	559199	560812	1	Putative protein of unknown function	Putative protein of unknown function; SWAT-GFP and mCherry fusion proteins localize to the cell periphery
S000004756	YMR148W	LDO16	LD Organization protein of 16kD	560366	560812	1	Protein of unknown function with possible role in spore wall assembly	Protein of unknown function with possible role in spore wall assembly; predicted to contain an N-terminal transmembrane domain; osw5 null mutant spores exhibit increased spore wall permeability and sensitivity to beta-glucanase digestion
S000004757	YMR149W	SWP1	Suppressor of a WbP1 mutation	560996	561856	1	Delta subunit of the oligosaccharyl transferase glycoprotein complex	Delta subunit of the oligosaccharyl transferase glycoprotein complex; complex is required for N-linked glycosylation of proteins in the endoplasmic reticulum
S000004758	YMR150C	IMP1	Inner Membrane Protease	561956	562528	-1	Catalytic subunit of mitochondrial inner membrane peptidase complex	Catalytic subunit of mitochondrial inner membrane peptidase complex; required for maturation of mitochondrial proteins of the intermembrane space; complex contains two catalytic subunits (Imp1p and Imp2p that differ in substrate specificty) and Som1p
S000004759	YMR151W	YIM2	""	562506	562943	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 5% of ORF overlaps the verified gene IMP1
S000004760	YMR152W	YIM1	""	563096	564193	1	Aldehyde reductase	Aldehyde reductase; involved in detoxification of lignocellulose-derived aldehydes; catalyzes the reduction of furan, aliphatic and aromatic aldehydes using either NADH or NADPH as co-factor; membrane of the quinone oxidoreductase subfamily of the medium-chain dehydrogenase/reductase family; null mutant displays sensitivity to DNA damaging agents; protein abundance increases in response to DNA replication stress
S000004761	YMR153C-A	""	""	565574	565909	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps 3' end of verified gene NUP53/YMR153W
S000004762	YMR153W	NUP53	NUclear Pore	564435	565862	1	FG-nucleoporin component of central core of nuclear pore complex (NPC)	FG-nucleoporin component of central core of nuclear pore complex (NPC); also part of the NPC nuclear basket; contributes directly to nucleocytoplasmic transport; involved in regulation of transcription and mitosis; induces membrane tubulation, which may contribute to nuclear pore assembly; NUP53 has a paralog, ASM4, that arose from the whole genome duplication
S000004763	YMR154C	RIM13	Regulator of IME2	565999	568182	-1	Calpain-like cysteine protease	Calpain-like cysteine protease; involved in proteolytic activation of Rim101p in response to alkaline pH; localizes to punctate structures in alkaline conditions and in vps4 mutant; has similarity to A. nidulans palB
S000004764	YMR155W	""	""	568551	570194	1	Putative protein of unknown function	Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen
S000004765	YMR156C	TPP1	Three Prime Phosphatase	570300	571016	-1	DNA 3'-phosphatase	DNA 3'-phosphatase; functions in repair of endogenous damage of double-stranded DNA, activity is specific for removal of 3' phosphates at strand breaks; similar to the l-2-haloacid dehalogenase superfamily; homolog of human polynucleotide kinase/3'-phosphatase
S000004766	YMR157C	AIM36	Altered Inheritance rate of Mitochondria	571277	572044	-1	Protein of unknown function	Protein of unknown function; null mutant displays reduced respiratory growth and elevated frequency of mitochondrial genome loss; the authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies
S000004767	YMR158W	MRPS8	Mitochondrial Ribosomal Protein, Small subunit	572248	572715	1	Mitochondrial ribosomal protein of the small subunit	Mitochondrial ribosomal protein of the small subunit
S000004768	YMR158W-B	""	""	574471	574791	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene ATG16/YMR159C
S000004769	YMR159C	ATG16	AuTophaGy related	574476	574928	-1	Conserved protein involved in autophagy	Conserved protein involved in autophagy; interacts with Atg12p-Atg5p conjugates to form Atg12p-Atg5p-Atg16p multimers, which binds to membranes and localizes to the pre-autophagosomal structure and are required for autophagy; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
S000004770	YMR160W	CVM1	Contact of the Vacuole Membrane	575066	577516	1	Protein involved in sphingolipid metabolism	Protein involved in sphingolipid metabolism; localizes to vacuolar contact sites with mitochondria, ER and peroxisomes; mutant has enhanced sensitivity to overexpression of mutant huntingtin; relative distribution within the vacuolar membrane changes upon DNA replication stress
S000004771	YMR161W	HLJ1	HomoLogous to E. coli dnaJ protein	577718	578392	1	Co-chaperone for Hsp40p	Co-chaperone for Hsp40p; anchored in the ER membrane; with its homolog Ydj1p promotes ER-associated protein degradation (ERAD) of integral membrane substrates; similar to E. coli DnaJ
S000004772	YMR162C	DNF3	Drs2 Neo1 Family	578951	583921	-1	Trans-golgi network aminophospholipid translocase (flippase)	Trans-golgi network aminophospholipid translocase (flippase); type 4 P-type ATPase; involved in phospholipid translocation, contributing to the maintenance of membrane lipid asymmetry in post-Golgi secretory vesicles; role in protein trafficking between the Golgi and endosomal system; localizes to the trans-Golgi network; localizes to the shmoo tip where it has a redundant role in the cellular response to mating pheromone
S000004773	YMR163C	INP2	INheritance of Peroxisomes	584271	586388	-1	Peroxisome-specific receptor important for peroxisome inheritance	Peroxisome-specific receptor important for peroxisome inheritance; co-fractionates with peroxisome membranes and co-localizes with peroxisomes in vivo; physically interacts with the myosin V motor Myo2p; INP2 is not an essential gene
S000004774	YMR164C	MSS11	Multicopy Suppressor of STA genes	587274	589550	-1	Transcription factor	Transcription factor; involved in regulation of invasive growth and starch degradation; controls the activation of FLO11 and STA2 in response to nutritional signals; forms a heterodimer with Flo8p that interacts with the Swi/Snf complex during transcriptional activation of FLO1, FLO11, and STA1
S000004776	YMR166C	MME1	Mitochondrial Magnesium Exporter	593367	594473	-1	Transporter of the mitochondrial inner membrane that exports magnesium	Transporter of the mitochondrial inner membrane that exports magnesium; involved in mitochondrial Mg2+ homeostasis; has similarity to human mitochondrial ATP-Mg/Pi carriers
S000004777	YMR167W	MLH1	MutL Homolog	594886	597195	1	Protein required for mismatch repair in mitosis and meiosis	Protein required for mismatch repair in mitosis and meiosis; also required for crossing over during meiosis; forms a complex with Pms1p and Msh2p-Msh3p during mismatch repair; required for silencing at the silent mating-type loci and telomeres; human homolog is associated with hereditary non-polyposis colon cancer
S000004778	YMR168C	CEP3	CEntromere Protein	597332	599158	-1	Essential kinetochore protein	Essential kinetochore protein; component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
S000004779	YMR169C	ALD3	ALdehyde Dehydrogenase	599352	600872	-1	Cytoplasmic aldehyde dehydrogenase	Cytoplasmic aldehyde dehydrogenase; involved in beta-alanine synthesis; uses NAD+ as the preferred coenzyme; very similar to Ald2p; expression is induced by stress and repressed by glucose
S000004780	YMR170C	ALD2	ALdehyde Dehydrogenase	601562	603082	-1	Cytoplasmic aldehyde dehydrogenase	Cytoplasmic aldehyde dehydrogenase; involved in ethanol oxidation and beta-alanine biosynthesis; uses NAD+ as the preferred coenzyme; expression is stress induced and glucose repressed; very similar to Ald3p
S000004781	YMR171C	EAR1	Endosomal Adaptor of Rsp5p	603868	605520	-1	Specificity factor required for Rsp5p-dependent ubiquitination	Specificity factor required for Rsp5p-dependent ubiquitination; also required for sorting of specific cargo proteins at the multivesicular body; mRNA is targeted to the bud via the mRNA transport system involving She2p
S000004782	YMR172C-A	""	""	607828	608211	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps 3' end of ORF HOT1/YMR172W
S000004783	YMR172W	HOT1	High-Osmolarity-induced Transcription	605981	608140	1	Transcription factor for glycerol biosynthetic genes	Transcription factor for glycerol biosynthetic genes; required for the transient induction of glycerol biosynthetic genes GPD1 and GPP2 in response to high osmolarity; targets Hog1p to osmostress responsive promoters; has similarity to Msn1p and Gcr1p
S000004784	YMR173W	DDR48	DNA Damage Responsive	608689	609981	1	DNA damage-responsive protein	DNA damage-responsive protein; expression is increased in response to heat-shock stress or treatments that produce DNA lesions; contains multiple repeats of the amino acid sequence NNNDSYGS; protein abundance increases in response to DNA replication stress
S000004785	YMR173W-A	""	""	608897	610081	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene DDR48/YML173W
S000004786	YMR174C	PAI3	Proteinase A Inhibitor	610159	610365	-1	Cytoplasmic proteinase A (Pep4p) inhibitor	Cytoplasmic proteinase A (Pep4p) inhibitor; dependent on Pbs2p and Hog1p protein kinases for osmotic induction; intrinsically unstructured, N-terminal half becomes ordered in the active site of proteinase A upon contact
S000004787	YMR175W	SIP18	Salt Induced Protein	611016	611255	1	Phospholipid-binding hydrophilin	Phospholipid-binding hydrophilin; essential to overcome desiccation-rehydration process; expression is induced by osmotic stress; SIP18 has a paralog, GRE1, that arose from the whole genome duplication
S000004788	YMR176W	ECM5	ExtraCellular Mutant	611740	615975	1	Subunit of the Snt2C complex	Subunit of the Snt2C complex; physically associates with Snt2p and Rpd3p; along with Snt2p, recruits Rpd3p to a small number of promoters; also colocalizes with Snt2p, independently of Rpd3p, to promoters of stress response genes in response to oxidative stress; contains ATP/GTP-binding site motif A; null mutant exhibits increased cellular volume, large drooping buds with elongated necks; relative distribution to the nucleus increases upon DNA replication stress
S000004789	YMR177W	MMT1	Mitochondrial Metal Transporter	616566	618098	1	Putative metal transporter involved in mitochondrial iron accumulation	Putative metal transporter involved in mitochondrial iron accumulation; MMT1 has a paralog, MMT2, that arose from the whole genome duplication
S000004790	YMR178W	FPY1	FAD PYrophosphatase	618479	619303	1	FAD pyrophosphatase	FAD pyrophosphatase; hydrolyzes FAD as the preferred substrate but also acts on NADH and ADP-ribose at lower rates; activity is dependent upon K+ and divalent metal cations; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus; FPY1 is not an essential gene; protein abundance increases in response to DNA replication stress
S000004791	YMR179W	SPT21	SuPpressor of Ty	619858	622134	1	Protein with a role in transcriptional silencing	Protein with a role in transcriptional silencing; required for normal transcription at several loci including HTA2-HTB2 and HHF2-HHT2, but not required at the other histone loci; binds with Spt10p to histone gene promoters during S phase; localizes to nuclear foci that become diffuse upon DNA replication stress
S000004792	YMR180C	CTL1	Capping enzyme mRNA Triphosphatase-Like	622251	623213	-1	RNA 5'-triphosphatase, localizes to both the nucleus and cytoplasm	RNA 5'-triphosphatase, localizes to both the nucleus and cytoplasm; CTL1 has a paralog, CET1, that arose from the whole genome duplication
S000004793	YMR181C	""	""	623616	624080	-1	Protein of unknown function	Protein of unknown function; mRNA transcribed as part of a bicistronic transcript with a predicted transcriptional repressor RGM1/YMR182C; mRNA is destroyed by nonsense-mediated decay (NMD); not an essential gene; YMR181C has a paralog, YPL229W, that arose from the whole genome duplication
S000004794	YMR182C	RGM1	""	624532	625167	-1	Putative zinc finger DNA binding transcription factor	Putative zinc finger DNA binding transcription factor; contains two N-terminal C2H2 zinc fingers and C-terminal proline rich domain; overproduction impairs cell growth and induces expression of genes involved in monosaccharide catabolism and aldehyde metabolism; regulates expression of of Y' telomeric elements and subtelomeric COS genes; relocalizes to the cytosol in response to hypoxia; RGM1 has a paralog, USV1, that arose from the whole genome duplication
S000004795	YMR183C	SSO2	Supressor of Sec One	626921	627808	-1	Plasma membrane t-SNARE	Plasma membrane t-SNARE; involved in fusion of secretory vesicles at the plasma membrane; syntaxin homolog that is functionally redundant with Sso1p; SSO2 has a paralog, SSO1, that arose from the whole genome duplication
S000004796	YMR184W	ADD37	Alpha1-proteinase inhibitor-Degradation Deficient	628189	628785	1	Protein of unknown function	Protein of unknown function; involved in ER-associated protein degradation; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YMR184W is not an essential gene; protein abundance increases in response to DNA replication stress
S000004797	YMR185W	RTP1	Required for the nuclear Transport of RNA Pol II	629025	631970	1	Protein required for the nuclear import and biogenesis of RNA pol II	Protein required for the nuclear import and biogenesis of RNA pol II; conflicting evidence on whether null mutant is viable with elongated buds, or inviable; interacts with Rpb2, Rpb3, Nup116p, Nup100p and components of the R2TP complex (Rvb1p, Rvb2p, Pih1p); similar to human TMCO7 gene
S000004798	YMR186W	HSC82	""	632355	634472	1	Cytoplasmic chaperone of the Hsp90 family	Cytoplasmic chaperone of the Hsp90 family; plays a role in determining prion variants; redundant in function and nearly identical with Hsp82p, and together they are essential; expressed constitutively at 10-fold higher basal levels than HSP82 and induced 2-3 fold by heat shock; contains two acid-rich unstructured regions that promote the solubility of chaperone-substrate complexes; HSC82 has a paralog, HSP82, that arose from the whole genome duplication
S000004799	YMR187C	""	""	634689	635984	-1	Putative protein of unknown function	Putative protein of unknown function; YMR187C is not an essential gene
S000004800	YMR188C	MRPS17	Mitochondrial Ribosomal Protein, Small subunit	636291	637004	-1	Mitochondrial ribosomal protein of the small subunit	Mitochondrial ribosomal protein of the small subunit
S000004801	YMR189W	GCV2	GlyCine cleaVage	637500	640604	1	P subunit of the mitochondrial glycine decarboxylase complex	P subunit of the mitochondrial glycine decarboxylase complex; glycine decarboxylase is required for the catabolism of glycine to 5,10-methylene-THF; expression is regulated by levels of 5,10-methylene-THF in the cytoplasm
S000004802	YMR190C	SGS1	Slow Growth Suppressor	640915	645258	-1	RecQ family nucleolar DNA helicase	RecQ family nucleolar DNA helicase; role in genome integrity maintenance, chromosome synapsis, meiotic joint molecule/crossover formation; stimulates activity of Top3p; rapidly lost in response to rapamycin in Rrd1p-dependent manner; forms nuclear foci upon DNA replication stress; yeast SGS1 complements mutations in human homolog BLM implicated in Bloom syndrome; also similar to human WRN implicated in Werner syndrome; human BLM and WRN can each complement yeast null mutant
S000004803	YMR191W	SPG5	Stationary Phase Gene	645656	646777	1	Protein required for proteasome assembly during quiescence	Protein required for proteasome assembly during quiescence; binds to base of the proteasome regulartory particle; required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources
S000004804	YMR192W	GYL1	GYp Like	647118	649280	1	Putative GTPase activating protein (GAP) with a role in exocytosis	Putative GTPase activating protein (GAP) with a role in exocytosis; stimulates Gyp5p GAP activity on Ypt1p, colocalizes with Gyp5p at sites of polarized growth; interacts with Gyp5p, Rvs161p, and Rvs167p; involved in recruiting Rvs167p to the bud tip during polarized growth; increases in abundance and relocalizes from bud neck to cytoplasm upon DNA replication stress; GYL1 has a paralog, GYP5, that arose from the whole genome duplication
S000004805	YMR193C-A	""	""	651072	651458	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000004806	YMR193W	MRPL24	Mitochondrial Ribosomal Protein, Large subunit	650036	650812	1	Mitochondrial ribosomal protein of the large subunit	Mitochondrial ribosomal protein of the large subunit; two mitochondrial ribosomal proteins, YmL14 and YmL24, have been assigned to the same gene
S000004807	YMR194W	RPL36A	Ribosomal Protein of the Large subunit	651145	651910	1	Ribosomal 60S subunit protein L36A	Ribosomal 60S subunit protein L36A; N-terminally acetylated; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36A has a paralog, RPL36B, that arose from the whole genome duplication
S000004808	YMR195W	ICY1	Interacting with the CYtoskeleton	654034	654417	1	Protein of unknown function	Protein of unknown function; required for viability in rich media of cells lacking mitochondrial DNA; mutants have an invasive growth defect with elongated morphology; induced by amino acid starvation; ICY1 has a paralog, ATG41, that arose from the whole genome duplication
S000004809	YMR196W	""	""	655076	658342	1	Putative protein of unknown function	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene
S000004810	YMR197C	VTI1	Vps10 (Ten) Interacting	658545	659198	-1	Protein involved in cis-Golgi membrane traffic	Protein involved in cis-Golgi membrane traffic; v-SNARE that interacts with two t-SNARES, Sed5p and Pep12p; required for multiple vacuolar sorting pathways; human homolog VTI1A can complement yeast null mutant
S000004811	YMR198W	CIK1	Chromosome Instability and Karyogamy	659745	661529	1	Kinesin-associated protein	Kinesin-associated protein; required for both karyogamy and mitotic spindle organization, interacts stably and specifically with Kar3p and may function to target this kinesin to a specific cellular role; locus encodes a long and short transcript with differing functions; CIK1 has a paralog, VIK1, that arose from the whole genome duplication
S000004812	YMR199W	CLN1	CycLiN	662644	664284	1	G1 cyclin involved in regulation of the cell cycle	G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p); CLN1 has a paralog, CLN2, that arose from the whole genome duplication; cell cycle arrest phenotype of the cln1 cln2 cln3 triple null mutant is complemented by any of human cyclins CCNA2, CCNB1, CCNC, CCND1, or CCNE1
S000004813	YMR200W	ROT1	Reversal Of Tor2 lethality	664752	665522	1	Molecular chaperone involved in protein folding in ER	Molecular chaperone involved in protein folding in ER; mutation causes defects in cell wall synthesis and lysis of autophagic bodies, suppresses tor2 mutations, and is synthetically lethal with kar2-1 and with rot2 mutations; involved in N-linked glycosylation and O-mannosylation; transmembrane helix Ser250 is essential for Rot1p to interact with other membrane components and exert its functional role, avoiding exposure of Ser H-bonding group at lipid-exposed surface
S000004814	YMR201C	RAD14	RADiation sensitive	665845	667044	-1	Subunit of Nucleotide Excision Repair Factor 1 (NEF1)	Subunit of Nucleotide Excision Repair Factor 1 (NEF1); recognizes and binds damaged DNA during nucleotide excision repair (NER); also involved in regulation of transcription; contains zinc finger motif; homolog of human XPA protein
S000004815	YMR202W	ERG2	ERGosterol biosynthesis	667537	668205	1	C-8 sterol isomerase	C-8 sterol isomerase; catalyzes isomerization of delta-8 double bond to delta-7 position at an intermediate step in ergosterol biosynthesis; transcriptionally down-regulated when ergosterol is in excess; mutation is functionally complemented by human EBP
S000004816	YMR203W	TOM40	Translocase of the Outer Mitochondrial membrane	668492	669655	1	Component of the TOM (translocase of outer membrane) complex	Component of the TOM (translocase of outer membrane) complex; responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore; pre-Tom40p is phosphorylated by PKA, which impairs its import into mitochondria under non-respiratory conditions
S000004817	YMR204C	INP1	INheritance of Peroxisomes	670063	671325	-1	Peripheral membrane protein of peroxisomes	Peripheral membrane protein of peroxisomes; involved in peroxisomal inheritance; recruitment to peroxisomes is mediated by interaction with Pex3p at the peroxisomal membrane; Inp1p and Pex3p tether peroxisomes to plasma membrane
S000004818	YMR205C	PFK2	PhosphoFructoKinase	671887	674766	-1	Beta subunit of heterooctameric phosphofructokinase	Beta subunit of heterooctameric phosphofructokinase; involved in glycolysis; indispensable for anaerobic growth; activated by fructose-2,6-bisphosphate and AMP; mutation inhibits glucose induction of cell cycle-related genes
S000004819	YMR206W	""	""	675896	676837	1	Putative protein of unknown function	Putative protein of unknown function; not an essential gene; YMR206W has a paralog, YNR014W, that arose from the whole genome duplication
S000004820	YMR207C	HFA1	""	677193	683564	-1	Mitochondrial acetyl-coenzyme A carboxylase	Mitochondrial acetyl-coenzyme A carboxylase; catalyzes production of malonyl-CoA in mitochondrial fatty acid biosynthesis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress; genetic and comparative analysis suggests that translation begins at a non-canonical (Ile) start codon at -372 relative to the annotated start codon
S000004821	YMR208W	ERG12	ERGosterol biosynthesis	684467	685798	1	Mevalonate kinase	Mevalonate kinase; acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate; human MVK functionally complements the lethality of the erg12 null mutation
S000004822	YMR209C	""	""	685911	687284	-1	Putative S-adenosylmethionine-dependent methyltransferase	Putative S-adenosylmethionine-dependent methyltransferase; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum and vacuole respectively; YMR209C is not an essential gene
S000004823	YMR210W	MGL2	MonoacylGlycerol Lipase	687516	688865	1	Monoacylglycerol and triacylglycerol lipase	Monoacylglycerol and triacylglycerol lipase; MAG lipase with preference for palmitoyl-MAG; TAG lipase involved in TAG catabolism; minor role in medium-chain fatty acid ethyl ester biosynthesis; contains an alpha/beta hydrolase domain and a typical lipase motif (GXSXG); similar to acyltransferases, Eeb1p, Eht1p, and human ABHD1
S000004824	YMR211W	DML1	Drosophila melanogaster Misato-Like protein	689083	690510	1	Essential protein involved in mtDNA inheritance	Essential protein involved in mtDNA inheritance; may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family
S000004826	YMR213W	CEF1	CErevisiae homolog of cdc Five	693381	695153	1	Essential splicing factor	Essential splicing factor; associated with Prp19p and the spliceosome, contains an N-terminal c-Myb DNA binding motif necessary for cell viability but not for Prp19p association, evolutionarily conserved and homologous to S. pombe Cdc5p
S000004827	YMR214W	SCJ1	S. Cerevisiae DnaJ	695350	696483	1	One of several homologs of bacterial chaperone DnaJ	One of several homologs of bacterial chaperone DnaJ; located in the ER lumen where it cooperates with Kar2p to mediate maturation of proteins
S000004828	YMR215W	GAS3	Glycophospholipid-Anchored Surface protein	696796	698370	1	Putative 1,3-beta-glucanosyltransferase	Putative 1,3-beta-glucanosyltransferase; has similarity go other GAS family members; low abundance, possibly inactive member of the GAS family of GPI-containing proteins; localizes to the cell wall; mRNA induced during sporulation
S000004829	YMR216C	SKY1	SRPK1-like Kinase in Yeast	698811	701039	-1	SR protein kinase (SRPK)	SR protein kinase (SRPK); involved in mRNA 3' splice site recognition with Prp8p and Cdc40p; phosphorylates one or more SR dipeptides on mRNA export factors Npl3p and Gbp2p to stimulate cytoplasmic release of mRNA and Mtr10p-mediated nuclear reimport; component of stress granules that regulates granule disassembly during recovery from stress; modulates cation uptake and homeostasis through membrane transporters; contains prion-like domains; similar to human SRPK1
S000004831	YMR218C	TRS130	TRapp Subunit	703579	706887	-1	Component of transport protein particle (TRAPP) complex II	Component of transport protein particle (TRAPP) complex II; TRAPPII is a multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating intra-Golgi and endosome-Golgi traffic
S000004832	YMR219W	ESC1	Establishes Silent Chromatin	707133	712109	1	Protein involved in telomeric silencing	Protein involved in telomeric silencing; required for quiescent cell telomere hypercluster localization at nuclear membrane vicinity; interacts with PAD4-domain of Sir4p
S000004833	YMR220W	ERG8	ERGosterol biosynthesis	712316	713671	1	Phosphomevalonate kinase	Phosphomevalonate kinase; an essential cytosolic enzyme that acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
S000004834	YMR221C	FMP42	Found in Mitochondrial Proteome	713931	715445	-1	Putative protein of unknown function	Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; physical interaction with Atg27p suggests a possible role in autophagy; targeted to vacuole via AP-3 pathway
S000004835	YMR222C	FSH2	Family of Serine Hydrolases	715638	716309	-1	Putative serine hydrolase that localizes to the cytoplasm	Putative serine hydrolase that localizes to the cytoplasm; sequence is similar to S. cerevisiae Fsh1p and Fsh3p and the human candidate tumor suppressor OVCA2
S000004836	YMR223W	UBP8	UBiquitin-specific processing Protease	716715	718130	1	Ubiquitin-specific protease component of the SAGA acetylation complex	Ubiquitin-specific protease component of the SAGA acetylation complex; required for SAGA (Spt-Ada-Gcn5-Acetyltransferase)-mediated deubiquitination of histone H2B
S000004837	YMR224C	MRE11	Meiotic REcombination	718575	720653	-1	Nuclease subunit of the MRX complex with Rad50p and Xrs2p	Nuclease subunit of the MRX complex with Rad50p and Xrs2p; complex functions in repair of DNA double-strand breaks and in telomere stability; Mre11p associates with Ser/Thr-rich ORFs in premeiotic phase; nuclease activity required for MRX function; widely conserved; forms nuclear foci upon DNA replication stress
S000004838	YMR225C	MRPL44	Mitochondrial Ribosomal Protein, Large subunit	720960	721403	-1	Mitochondrial ribosomal protein of the large subunit	Mitochondrial ribosomal protein of the large subunit; protein abundance increases in response to DNA replication stress
S000004839	YMR226C	ORA1	OxidoReductase of Alcohols	721593	722396	-1	NADP(+)-dependent serine dehydrogenase and carbonyl reductase	NADP(+)-dependent serine dehydrogenase and carbonyl reductase; acts on serine, L-allo-threonine, and other 3-hydroxy acids; catalyzes the reduction of (S)-α-acetolactate to 2,3-dimethylglycerate; short-chain dehydrogenase/reductase (SDR) family member; GFP fusion protein localizes to the cytoplasm and nucleus
S000004840	YMR227C	TAF7	TATA binding protein-Associated Factor	722613	724385	-1	TFIID subunit (67 kDa)	TFIID subunit (67 kDa); involved in RNA polymerase II transcription initiation
S000004841	YMR228W	MTF1	Mitochondrial Transcription Factor	724626	725651	1	Mitochondrial RNA polymerase specificity factor	Mitochondrial RNA polymerase specificity factor; has structural similarity to S-adenosylmethionine-dependent methyltransferases and functional similarity to bacterial sigma-factors; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation
S000004842	YMR229C	RRP5	Ribosomal RNA Processing	725934	731123	-1	RNA binding protein involved in synthesis of 18S and 5.8S rRNAs	RNA binding protein involved in synthesis of 18S and 5.8S rRNAs; component of ribosomal small subunit (SSU) processome and 90S preribosome; required for pre-rRNA packaging and compaction of processome into dense terminal balls; part of Mak21p-Noc2p-Rrp5p module that associates with nascent pre-rRNA during transcription with role in biogenesis of large ribosomal subunit; binds single stranded tracts of U's; relocalizes from nucleolus to nucleus upon DNA replication stress
S000004843	YMR230W	RPS10B	Ribosomal Protein of the Small subunit	732414	733141	1	Protein component of the small (40S) ribosomal subunit	Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10B has a paralog, RPS10A, that arose from the whole genome duplication; mutations in the human homolog associated with Diamond-Blackfan anemia
S000004844	YMR231W	PEP5	carboxyPEPtidase Y-deficient	733545	736634	1	Histone E3 ligase, component of CORVET membrane tethering complex	Histone E3 ligase, component of CORVET membrane tethering complex; peripheral vacuolar membrane protein required for protein trafficking and vacuole biogenesis; interacts with Pep7p; involved in ubiquitination and degradation of excess histones
S000004845	YMR232W	FUS2	cell FUSion	736926	738959	1	Cell fusion regulator	Cell fusion regulator; cytoplasmic protein localized to shmoo tip; required for alignment of parental nuclei before nuclear fusion during mating; contains a Dbl-homology domain; binds specifically with activated Cdc42p
S000004846	YMR233W	TRI1	""	739185	739865	1	Non-essential sumoylated protein of unknown function	Non-essential sumoylated protein of unknown function; similar to components of human SWI/SNF complex including SMRD3; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm, nucleus and nucleolus; TRI1 has a paralog, UAF30, that arose from the whole genome duplication
S000004847	YMR234W	RNH1	RNase H	740266	741312	1	Ribonuclease H1	Ribonuclease H1; able to bind double-stranded RNAs and RNA-DNA hybrids; associates with RNAse polymerase I.
S000004848	YMR235C	RNA1	rapid cessation of net RNA accumulation	741512	742735	-1	GTPase activating protein (GAP) for Gsp1p	GTPase activating protein (GAP) for Gsp1p; involved in nuclear transport
S000004849	YMR236W	TAF9	TATA binding protein-Associated Factor	742971	743444	1	Subunit (17 kDa) of TFIID and SAGA complexes	Subunit (17 kDa) of TFIID and SAGA complexes; involved in RNA polymerase II transcription initiation and in chromatin modification, similar to histone H3
S000004850	YMR237W	BCH1	Bud7 and Chs6 Homolog	743749	745923	1	Member of the ChAPs family (Chs5p-Arf1p-binding proteins)	Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins from the Golgi to the plasma membrane; interacts selectively with the activated, GTP-bound form of Arf1p; may interact with ribosomes; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress
S000004851	YMR238W	DFG5	Defective for Filamentous Growth	746353	747729	1	Putative mannosidase	Putative mannosidase; essential glycosylphosphatidylinositol (GPI)-anchored membrane protein required for cell wall biogenesis in bud formation, involved in filamentous growth, homologous to Dcw1p
S000004852	YMR239C	RNT1	RNase Three	748262	749677	-1	Nuclear dsRNA-specific ribonuclease (RNase III)	Nuclear dsRNA-specific ribonuclease (RNase III); involved in rDNA transcription, rRNA processing and U2 snRNA 3' end formation by cleavage of a stem-loop structure at the 3' end of U2 snRNA; involved in polyadenylation-independent transcription termination; involved in the cell wall stress response, regulating the degradation of cell wall integrity and morphogenesis checkpoint genes
S000004853	YMR240C	CUS1	Cold sensitive U2 snRNA Suppressor	749930	751240	-1	Protein required for assembly of U2 snRNP into the spliceosome	Protein required for assembly of U2 snRNP into the spliceosome; forms a complex with Hsh49p and Hsh155p
S000004854	YMR241W	YHM2	Yeast suppressor of HM mutant	751961	752905	1	Citrate and oxoglutarate carrier protein	Citrate and oxoglutarate carrier protein; exports citrate from and imports oxoglutarate into the mitochondrion, causing net export of NADPH reducing equivalents; also associates with mt nucleoids and has a role in replication and segregation of the mt genome
S000004855	YMR242C	RPL20A	Ribosomal Protein of the Large subunit	753225	754220	-1	Ribosomal 60S subunit protein L20A	Ribosomal 60S subunit protein L20A; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20A has a paralog, RPL20B, that arose from the whole genome duplication
S000004856	YMR243C	ZRC1	Zinc Resistance Conferring	754838	756166	-1	Vacuolar membrane zinc transporter	Vacuolar membrane zinc transporter; transports zinc from cytosol to vacuole for storage; also has role in resistance to zinc shock resulting from sudden influx of zinc into cytoplasm; human ortholog SLC30A10 functions as a Mn transporter and mutations in SLC30A10 cause neurotoxic accumulation of Mn in liver and brain; ZRC1 has a paralog, COT1, that arose from the whole genome duplication
S000004857	YMR244C-A	COA6	Cytochrome Oxidase Assembly	758517	758831	-1	Protein involved in cytochrome c oxidase (Complex IV) assembly	Protein involved in cytochrome c oxidase (Complex IV) assembly; involved in delivery of copper to Complex IV; also required for efficient formation of respiratory supercomplexes comprised of Complexes III and IV; localizes to the mitochondrial intermembrane space; ortholog implicated in cardiac defects in zebrafish and human; transcription is induced in response to the DNA-damaging agent MMS; protein abundance increases in response to DNA replication stress
S000004858	YMR244W	""	""	757250	758317	1	Putative protein of unknown function	Putative protein of unknown function; SWAT-GFP fusion protein localizes to the endoplasmic reticulum and vacuole, while mCherry fusion localizes to just the vacuole
S000004859	YMR245W	""	""	758563	759183	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000004860	YMR246W	FAA4	Fatty Acid Activation	759807	761891	1	Long chain fatty acyl-CoA synthetase	Long chain fatty acyl-CoA synthetase; activates fatty acids with a preference for C12:0-C16:0 chain lengths; role in the competitive import of long-chain fatty acids and sphingoid long-chain bases; role in stationary phase survival; localizes to lipid particles and the plasma membrane; role in sphingolipid-to-glycerolipid metabolism; forms cytoplasmic foci upon replication stress; faa1 faa4 double null complemented by any of human ACSBG1, ACSL1, 3, 4, 5, 6, SLC27A2, or 4
S000004861	YMR247C	RKR1	RING domain mutant Killed by Rtf1 deletion	763351	768039	-1	RING domain E3 ubiquitin ligase	RING domain E3 ubiquitin ligase; involved in ubiquitin-mediated degradation of non-stop proteins and translationally stalled ER membrane proteins; component of ribosome-bound RQC (ribosome quality control) complex; degrades products of mRNAs lacking a termination codon regardless of a poly(A) tail; functional connections to chromatin modification; homolog of mouse Listerin, mutations in which reported to cause neurodegeneration
S000004862	YMR250W	GAD1	GlutAmate Decarboxylase	770801	772558	1	Glutamate decarboxylase	Glutamate decarboxylase; converts glutamate into gamma-aminobutyric acid (GABA) during glutamate catabolism; involved in response to oxidative stress
S000004863	YMR251W	GTO3	Glutathione Transferase Omega-like	772915	774015	1	Omega class glutathione transferase	Omega class glutathione transferase; putative cytosolic localization
S000004864	YMR251W-A	HOR7	HyperOsmolarity-Responsive	774752	774931	1	Protein of unknown function	Protein of unknown function; overexpression suppresses Ca2+ sensitivity of mutants lacking inositol phosphorylceramide mannosyltransferases Csg1p and Csh1p; transcription is induced under hyperosmotic stress and repressed by alpha factor; HOR7 has a paralog, DDR2, that arose from the whole genome duplication
S000004865	YMR252C	MLO1	Mitochondrially LOcalized protein	775315	775719	-1	Mitochondrial protein of unknown function	Mitochondrial protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; MLO1 is not an essential gene
S000004866	YMR253C	""	""	775946	777190	-1	Putative membrane protein, involved in salt tolerance	Putative membrane protein, involved in salt tolerance; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern; YMR253C is not an essential gene
S000004867	YMR254C	""	""	777615	777923	-1	Putative protein of unknown function	Putative protein of unknown function; conserved across S. cerevisiae strains
S000004868	YMR255W	GFD1	Good For Dbp5p	778001	778567	1	Coiled-coiled protein of unknown function	Coiled-coiled protein of unknown function; identified as a high-copy suppressor of a dbp5 mutation; protein abundance increases in response to DNA replication stress
S000004869	YMR256C	COX7	Cytochrome c OXidase	778945	779127	-1	Subunit VII of cytochrome c oxidase (Complex IV)	Subunit VII of cytochrome c oxidase (Complex IV); Complex IV is the terminal member of the mitochondrial inner membrane electron transport chain
S000004870	YMR257C	PET111	PETite colonies	779629	782031	-1	Mitochondrial translational activator specific for the COX2 mRNA	Mitochondrial translational activator specific for the COX2 mRNA; located in the mitochondrial inner membrane
S000004871	YMR258C	ROY1	Repressor Of Ypt52	782620	784281	-1	GTPase inhibitor with similarity to F-box proteins	GTPase inhibitor with similarity to F-box proteins; inhibits Ypt52p GTPase activity by preventing Ypt52p from binding GTP; involved in regulating intracellular trafficking; physically interacts with Skp1p
S000004872	YMR259C	TRM732	Transfer RNA Methyltransferase	784621	788883	-1	Protein involved in 2'-O-methylation of C32 of substrate tRNAs	Protein involved in 2'-O-methylation of C32 of substrate tRNAs; interacts with 2'-O-ribose methyltransferase Trm7p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; non-essential gene; yeast null mutant can be functionally complemented by human THADA, mutations in which have been implicated in epithelial thyroid adenomas, type 2 diabetes, and polycystic ovary syndrome (PCOS)
S000004873	YMR260C	TIF11	Translation Initiation Factor	789378	789839	-1	Translation initiation factor eIF1A	Translation initiation factor eIF1A; essential protein that forms a complex with Sui1p (eIF1) and 40S ribosomal subunit and scans for start codons; C-terminus associates with Fun12p (eIF5B); N terminus interacts with eIF2 and eIF3; ortholog of bacterial translation initiation factor IF1
S000004874	YMR261C	TPS3	Trehalose Phosphate Synthase	790205	793369	-1	Regulatory subunit of trehalose-6-phosphate synthase/phosphatase	Regulatory subunit of trehalose-6-phosphate synthase/phosphatase; involved in synthesis of storage carbohydrate trehalose; expression is induced by stress conditions and repressed by the Ras-cAMP pathway; TPS3 has a paralog, TSL1, that arose from the whole genome duplication
S000004875	YMR262W	""	""	793726	794667	1	Protein of unknown function	Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene
S000004876	YMR263W	SAP30	Sin3-Associated Polypeptide	794919	795524	1	Component of Rpd3L histone deacetylase complex	Component of Rpd3L histone deacetylase complex; involved in silencing at telomeres, rDNA, and silent mating-type loci; involved in telomere maintenance
S000004877	YMR264W	CUE1	Coupling of Ubiquitin conjugation to ER degradation	795805	796416	1	Ubiquitin-binding protein	Ubiquitin-binding protein; ER membrane protein that recruits and integrates the ubiquitin-conjugating enzyme Ubc7p into ER membrane-bound ubiquitin ligase complexes that function in the ER-associated degradation (ERAD) pathway for misfolded proteins; contains a CUE domain that binds ubiquitin to facilitate intramolecular monoubiquitination and to promote diubiquitin elongation, facilitating polyubiquitin chain formation
S000004878	YMR265C	""	""	796540	797925	-1	Putative protein of unknown function	Putative protein of unknown function
S000004879	YMR266W	RSN1	Rescue of Sro7 at high Nacl	798518	801379	1	Membrane protein of unknown function	Membrane protein of unknown function; overexpression suppresses NaCl sensitivity of sro7 mutant cells by restoring sodium pump (Ena1p) localization to the plasma membrane
S000004880	YMR267W	PPA2	PyroPhosphatAse	801772	802704	1	Mitochondrial inorganic pyrophosphatase	Mitochondrial inorganic pyrophosphatase; required for mitochondrial function and possibly involved in energy generation from inorganic pyrophosphate; human ortholog, PPA2, functionally complements the null mutant; mutations in human PPA2 cause a mitochondrial disease resulting in sudden unexpected cardiac arrest in infants
S000004881	YMR268C	PRP24	Pre-mRNA Processing	802888	804222	-1	Splicing factor that reanneals snRNPs during spliceosome recycling	Splicing factor that reanneals snRNPs during spliceosome recycling; reanneals U4 and U6 snRNPs
S000004882	YMR269W	TMA23	Translation Machinery Associated	804456	805091	1	Nucleolar protein implicated in ribosome biogenesis	Nucleolar protein implicated in ribosome biogenesis; deletion extends chronological lifespan
S000004883	YMR270C	RRN9	Regulation of RNA polymerase I	805326	806423	-1	Protein involved in promoting high level transcription of rDNA	Protein involved in promoting high level transcription of rDNA; subunit of UAF (upstream activation factor) for RNA polymerase I
S000004884	YMR271C	URA10	URAcil requiring	806865	807548	-1	Minor orotate phosphoribosyltransferase (OPRTase) isozyme	Minor orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in the de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA10 has a paralog, URA5, that arose from the whole genome duplication
S000004885	YMR272C	SCS7	Suppressor of Ca2+ Sensitivity	809623	810777	-1	Sphingolipid alpha-hydroxylase	Sphingolipid alpha-hydroxylase; functions in the alpha-hydroxylation of sphingolipid-associated very long chain fatty acids, has both cytochrome b5-like and hydroxylase/desaturase domains, not essential for growth
S000004886	YMR273C	ZDS1	Zillion Different Screens	811233	813980	-1	Protein with a role in regulating Swe1p-dependent polarized growth	Protein with a role in regulating Swe1p-dependent polarized growth; involved in maintaining Cdc55p in the cytoplasm where it promotes mitotic entry; involved in mitotic exit through Cdc14p regulation; interacts with silencing proteins at telomeres; has a role in Bcy1p localization; implicated in mRNA nuclear export; ZDS1 has a paralog, ZDS2, that arose from the whole genome duplication
S000004887	YMR274C	RCE1	Ras and a-factor Converting Enzyme	814364	815311	-1	Type II CAAX prenyl protease	Type II CAAX prenyl protease; involved in the proteolysis and maturation of Ras and the a-factor mating pheromone
S000004888	YMR275C	BUL1	Binds Ubiquitin Ligase	815651	818581	-1	Alpha-arrestin, component of the Rsp5p E3-ubiquitin ligase complex	Alpha-arrestin, component of the Rsp5p E3-ubiquitin ligase complex; ubiquitin-binding adaptor involved in down-regulation of general amino acid transporter Gap1p upon shift to preferred nitrogen source; involved in lactate transporter Jen1p endocytosis; BUL1 has a paralog, BUL2, that arose from the whole genome duplication
S000004889	YMR276W	DSK2	Dominant Suppressor of kar1	818827	819948	1	Nuclear-enriched ubiquitin-like polyubiquitin-binding protein	Nuclear-enriched ubiquitin-like polyubiquitin-binding protein; K48-Ub chain binding protein that also binds K63-linked chains in vitro; required for spindle pole body (SPB) duplication and for transit through the G2/M phase of the cell cycle; involved in proteolysis; interacts with the proteasome; contains an N-terminal Ub-like (UBL) and a C-terminal Ub-associated (UBA) domain; protein abundance increases in response to DNA replication stress
S000004890	YMR277W	FCP1	tfiiF-associating component of Ctd Phosphatase	820256	822454	1	Carboxy-terminal domain (CTD) phosphatase	Carboxy-terminal domain (CTD) phosphatase; essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p); relocalizes to the cytosol in response to hypoxia
S000004891	YMR278W	PRM15	Phospho Ribo Mutase	822763	824631	1	Phosphoribomutase	Phosphoribomutase; catalyzes interconversion of ribose-1-phosphate and ribose-5-phosphate; has some phosphoglucomutase activity but primary activity in vivo is phosphoribomutase; contributes to ribose recycling in the pentose phosphate pathway; transcription induced in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; non-essential
S000004892	YMR279C	ATR2	AminoTriazole Resistance	824729	826351	-1	Putative boron transporter involved in boron efflux and resistance	Putative boron transporter involved in boron efflux and resistance; overexpression mutant but not null mutant displays boron tolerance phenotype; identified as a heat-induced gene in a high-throughout screen; YMR279C is not an essential gene; YMR279C has a paralog, ATR1, that arose from the whole genome duplication
S000004893	YMR280C	CAT8	CATabolite repression	827028	831329	-1	Zinc cluster transcriptional activator	Zinc cluster transcriptional activator; necessary for derepression of a variety of genes under non-fermentative growth conditions, active after diauxic shift, binds carbon source responsive elements; relative distribution to the nucleus increases upon DNA replication stress
S000004894	YMR281W	GPI12	GlycosylPhosphatidylInositol anchor biosynthesis	832339	833253	1	ER membrane protein involved in the second step of GPI anchor assembly	ER membrane protein involved in the second step of GPI anchor assembly; the second step is the de-N-acetylation of the N-acetylglucosaminylphosphatidylinositol intermediate; functional homolog of human PIG-Lp; GPI stands for glycosylphosphatidylinositol
S000004895	YMR282C	AEP2	ATPase ExPression	833356	835098	-1	Mitochondrial protein	Mitochondrial protein; likely involved in translation of the mitochondrial OLI1 mRNA; exhibits genetic interaction with the OLI1 mRNA 5'-untranslated leader
S000004896	YMR283C	RIT1	Ribosylation of Initiator tRNA	835326	836867	-1	Initiator methionine 2'-O-ribosyl phosphate transferase	Initiator methionine 2'-O-ribosyl phosphate transferase; modifies the initiator methionine tRNA at position 64 to distinguish it from elongator methionine tRNA
S000004897	YMR284W	YKU70	Yeast KU protein	838187	839995	1	Subunit of the telomeric Ku complex (Yku70p-Yku80p)	Subunit of the telomeric Ku complex (Yku70p-Yku80p); involved in telomere length maintenance, structure and telomere position effect; required for localization of telomerase ribonucleoprotein to nucleus via interaction with the TLC1 guide RNA; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair
S000004898	YMR285C	NGL2	""	840144	841691	-1	Protein involved in 5.8S rRNA processing	Protein involved in 5.8S rRNA processing; Ccr4p-like RNase required for correct 3'-end formation of 5.8S rRNA at site E; similar to Ngl1p; NGL2 has a paralog, NGL3, that arose from the whole genome duplication
S000004899	YMR286W	MRPL33	Mitochondrial Ribosomal Protein, Large subunit	841942	842202	1	Mitochondrial ribosomal protein of the large subunit	Mitochondrial ribosomal protein of the large subunit
S000004900	YMR287C	DSS1	Deletion of SUV3 Supressor	842436	845345	-1	3'-5' exoribonuclease	3'-5' exoribonuclease; component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs
S000004901	YMR288W	HSH155	Human Sap Homolog	845571	848486	1	U2-snRNP associated splicing factor	U2-snRNP associated splicing factor; forms extensive associations with the branch site-3' splice site-3' exon region upon prespliceosome formation; similarity to the mammalian U2 snRNP-associated splicing factor SAP155
S000004902	YMR289W	ABZ2	para-AminoBenZoic acid (PABA) biosynthesis	848685	849809	1	Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase)	Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase); catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis
S000004903	YMR290C	HAS1	Helicase Associated with Set1	850074	851591	-1	ATP-dependent RNA helicase	ATP-dependent RNA helicase; involved in the biogenesis of 40S and 60S ribosome subunits; localizes to both the nuclear periphery and nucleolus; highly enriched in nuclear pore complex fractions; constituent of 66S pre-ribosomal particles
S000004905	YMR291W	TDA1	Topoisomerase I Damage Affected	852630	854390	1	Protein kinase of unknown cellular role	Protein kinase of unknown cellular role; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; null mutant is sensitive to expression of the top1-T722A allele; not an essential gene; relocalizes from nucleus to cytoplasm upon DNA replication stress
S000004906	YMR292W	GOT1	GOlgi Transport	854795	855293	1	Homodimeric protein that is packaged into COPII vesicles	Homodimeric protein that is packaged into COPII vesicles; cycles between the ER and Golgi; involved in secretory transport but not directly required for aspects of transport assayed in vitro; may influence membrane composition
S000004907	YMR293C	HER2	Hmg2p ER Remodeling	855399	856793	-1	Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex	Subunit of the trimeric GatFAB AmidoTransferase(AdT) complex; involved in the formation of Q-tRNAQ; required for remodeling of ER caused by Hmg2p overexpression; similar to bacterial GatA glutamyl-tRNA amidotransferase
S000004908	YMR294W	JNM1	Just Nuclear Migration	856966	858087	1	Component of the yeast dynactin complex	Component of the yeast dynactin complex; consisting of Nip100p, Jnm1p, and Arp1p; required for proper nuclear migration and spindle partitioning during mitotic anaphase B
S000004909	YMR294W-A	""	""	858209	858568	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; substantially overlaps YMR295C; deletion causes sensitivity to unfolded protein response-inducing agents
S000004910	YMR295C	""	""	858297	858890	-1	Protein of unknown function that associates with ribosomes	Protein of unknown function that associates with ribosomes; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and bud; not an essential gene; protein abundance increases in response to DNA replication stress; YMR295C has a paralog, YGR273C, that arose from the whole genome duplication
S000004912	YMR297W	PRC1	PRoteinase C	861922	863520	1	Vacuolar carboxypeptidase Y (proteinase C, CPY)	Vacuolar carboxypeptidase Y (proteinase C, CPY); broad-specificity C-terminal exopeptidase involved in non-specific protein degradation in vacuole; involved, along with functional homolog Prc1p, in vacuolar zymogen activation, breakdown of autophagic body, and autophagosome-dependent protein synthesis; role in phytochelatin synthesis; targeted to vacuole via Vps10p-dependent endosomal vacuolar protein sorting pathway; member of serine carboxypeptidase family; N-glycosylated
S000004913	YMR298W	LIP1	Lag1p/Lac1p Interacting Protein	863819	864271	1	Ceramide synthase subunit	Ceramide synthase subunit; single-span ER membrane protein associated with Lag1p and Lac1p and required for ceramide synthase activity, null mutant grows extremely slowly and is defective in ceramide synthesis
S000004914	YMR299C	DYN3	DYNein	864412	865350	-1	Dynein light intermediate chain (LIC)	Dynein light intermediate chain (LIC); localizes with dynein, null mutant is defective in nuclear migration
S000004915	YMR300C	ADE4	ADEnine requiring	865559	867091	-1	Phosphoribosylpyrophosphate amidotransferase (PRPPAT)	Phosphoribosylpyrophosphate amidotransferase (PRPPAT); catalyzes first step of the 'de novo' purine nucleotide biosynthetic pathway; also known as amidophosphoribosyltransferase
S000004916	YMR301C	ATM1	ABC Transporter, Mitochondrial	867555	869627	-1	Mitochondrial inner membrane ATP-binding cassette (ABC) transporter	Mitochondrial inner membrane ATP-binding cassette (ABC) transporter; exports mitochondrially synthesized precursors of iron-sulfur (Fe/S) clusters to the cytosol; human homolog ABCB7 can complement yeast null mutant
S000004917	YMR302C	YME2	Yeast Mitochondrial Escape	870073	872625	-1	Integral inner mitochondrial membrane protein	Integral inner mitochondrial membrane protein; role in maintaining mitochondrial nucleoid structure and number; mutants exhibit an increased rate of mitochondrial DNA escape; shows some sequence similarity to exonucleases
S000004918	YMR303C	ADH2	Alcohol DeHydrogenase	873291	874337	-1	Glucose-repressible alcohol dehydrogenase II	Glucose-repressible alcohol dehydrogenase II; catalyzes the conversion of ethanol to acetaldehyde; involved in the production of certain carboxylate esters; regulated by ADR1
S000004919	YMR304C-A	""	""	878780	879130	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 3' end of verified ORF SCW10/YMR305C
S000004920	YMR304W	UBP15	UBiquitin-specific Protease	874987	878679	1	Ubiquitin-specific protease involved in protein deubiquitination	Ubiquitin-specific protease involved in protein deubiquitination; forms a complex with AAA peroxins Pex1p and Pex6p; deubiquitinates mono- and polyubiquitinated forms of Pex5p; deubiquitinates Clb5p, counteracting APC activity, and facilitating both Clb5p accumulation and S phase entry; physically interacts with anaphase-promoting complex/cyclosome (APC/C) activator, Cdh1p; catalytic activity regulated by an N-terminal TRAF-like domain and and C-terminal sequences
S000004921	YMR305C	SCW10	Soluble Cell Wall protein	879063	880232	-1	Cell wall protein with similarity to glucanases	Cell wall protein with similarity to glucanases; may play a role in conjugation during mating based on mutant phenotype and its regulation by Ste12p; SWC10 has a paralog, SCW4, that arose from the whole genome duplication
S000004922	YMR306C-A	""	""	886183	886572	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps 3' end of ORF FKS3/YMR306W
S000004923	YMR306W	FKS3	FK506 Sensitivity	881159	886516	1	Protein involved in spore wall assembly	Protein involved in spore wall assembly; has similarity to 1,3-beta-D-glucan synthase catalytic subunits Fks1p and Gsc2p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
S000004924	YMR307W	GAS1	Glycophospholipid-Anchored Surface protein	887003	888682	1	Beta-1,3-glucanosyltransferase required for cell wall assembly	Beta-1,3-glucanosyltransferase required for cell wall assembly; also has role in transcriptional silencing; localizes to cell surface via glycosylphosphatidylinositol (GPI) anchor, also found at nuclear periphery; genetic interactions with histone H3 lysine acetyltransferases GCN5 and SAS3 indicate functions for Gas1p in DNA damage response and cell cycle regulation; protein demonstrates amyloid properties in vivo
S000004925	YMR308C	PSE1	Protein Secretion Enhancer	888952	892221	-1	Karyopherin/importin that interacts with the nuclear pore complex	Karyopherin/importin that interacts with the nuclear pore complex; acts as the nuclear import receptor for specific proteins, including Pdr1p, Yap1p, Ste12p, and Aft1p; required for stability of the Dam1p-Duo1p complex
S000004926	YMR309C	NIP1	Nuclear ImPort	892988	895426	-1	eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3)	eIF3c subunit of the eukaryotic translation initiation factor 3 (eIF3); involved in the assembly of preinitiation complex and start codon selection; eIF3 is also involved in programmed stop codon readthrough
S000004927	YMR310C	UPA2	Urb2 particle associated methyl-transferase	895716	896669	-1	Putative methyltransferase	Putative methyltransferase; predicted to be involved in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; not an essential gene; YMR310C has a paralog, YGR283C, that arose from the whole genome duplication
S000004928	YMR311C	GLC8	GLyCogen	896914	897603	-1	Regulatory subunit of protein phosphatase 1 (Glc7p)	Regulatory subunit of protein phosphatase 1 (Glc7p); involved in glycogen metabolism and chromosome segregation; proposed to regulate Glc7p activity via conformational alteration; ortholog of the mammalian protein phosphatase inhibitor 2; protein abundance increases in response to DNA replication stress
S000004929	YMR312W	ELP6	ELongator Protein	898405	899226	1	Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex	Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity
S000004930	YMR313C	TGL3	TriacylGlycerol Lipase	899379	901307	-1	Bifunctional triacylglycerol lipase and LPE acyltransferase	Bifunctional triacylglycerol lipase and LPE acyltransferase; major lipid particle-localized triacylglycerol (TAG) lipase; catalyzes acylation of lysophosphatidylethanolamine (LPE), a function which is essential for sporulation; protein level and stability of Tgl3p are markedly reduced in the absence of lipid droplets; required with Tgl4p for timely bud formation
S000004931	YMR314W	PRE5	PRoteinase yscE	901709	902413	1	Alpha 6 subunit of the 20S proteasome	Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
S000004932	YMR315W	""	""	902800	903849	1	Protein with NADP(H) oxidoreductase activity	Protein with NADP(H) oxidoreductase activity; transcription is regulated by Stb5p in response to NADPH depletion induced by diamide; promoter contains an Stb5p binding site and Stb5p binding is enriched at the promoter; negative regulator of nonselective autophagy; protein abundance increases in response to DNA replication stress
S000004933	YMR316C-A	""	""	905662	905973	-1	Protein of unknown function	Protein of unknown function; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum; mRNA identified as translated by ribosome profiling data; overlaps the verified gene DIA1/YMR316W
S000004934	YMR316C-B	""	""	907321	907629	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps 5' end of ORF YMR317W
S000004935	YMR316W	DIA1	Digs Into Agar	904825	905835	1	Protein of unknown function	Protein of unknown function; involved in invasive and pseudohyphal growth; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
S000004936	YMR317W	""	""	907364	910786	1	Putative protein of unknown function	Putative protein of unknown function; has some similarity to sialidase from Trypanosoma; YMR317W is not an essential gene
S000004937	YMR318C	ADH6	Alcohol DeHydrogenase	911061	912143	-1	NADPH-dependent medium chain alcohol dehydrogenase	NADPH-dependent medium chain alcohol dehydrogenase; has broad substrate specificity; member of the cinnamyl family of alcohol dehydrogenases; may be involved in fusel alcohol synthesis or in aldehyde tolerance; protein abundance increases in response to DNA replication stress
S000004938	YMR319C	FET4	FErrous Transport	912880	914538	-1	Low-affinity Fe(II) transporter of the plasma membrane	Low-affinity Fe(II) transporter of the plasma membrane
S000004939	YMR320W	""	""	916746	917051	1	Putative protein of unknown function	Putative protein of unknown function; conserved among S. cerevisiae strains; YMR320W is not an essential gene
S000004941	YMR322C	SNO4	SNZ proximal Open reading frame	918366	919079	-1	Possible chaperone and cysteine protease	Possible chaperone and cysteine protease; required for transcriptional reprogramming during the diauxic shift and for survival in stationary phase; similar to bacterial Hsp31 and yeast Hsp31p, Hsp32p, and Hsp33p; DJ-1/ThiJ/PfpI superfamily member; predicted involvement in pyridoxine metabolism; induced by mild heat stress and copper deprivation
S000004945	YMR326C	""	""	923494	923802	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the telomere on the right arm of chromosome 13
S000004946	YNL001W	DOM34	Duplication Of Multilocus region	627456	628616	1	Protein that facilitates ribosomal subunit dissociation	Protein that facilitates ribosomal subunit dissociation; Dom34-Hbs1 complex and Rli1p have roles in dissociating inactive ribosomes to facilitate translation restart, particularly ribosomes stalled in 3' UTRs; required for RNA cleavage in no-go decay, but reports conflict on endonuclease activity; Pelota ortholog; protein abundance increases in response to DNA replication stress; DOM34 has a paralog, YCL001W-B, that arose from the whole genome duplication
S000004947	YNL002C	RLP7	Ribosomal-Like Protein	626174	627142	-1	Nucleolar protein similar to large ribosomal subunit L7 proteins	Nucleolar protein similar to large ribosomal subunit L7 proteins; constituent of 66S pre-ribosomal particles; plays an essential role in processing of precursors to the large ribosomal subunit RNAs; binds junction of ITS2 and ITS2-proximal stem between the 3' end of 5.8S rRNA and the 5' end of 25S rRNA
S000004948	YNL003C	PET8	PETite colonies	624975	625829	-1	S-adenosylmethionine transporter of the mitochondrial inner membrane	S-adenosylmethionine transporter of the mitochondrial inner membrane; member of the mitochondrial carrier family; required for biotin biosynthesis and respiratory growth
S000004949	YNL004W	HRB1	Hypothetical RNA-Binding protein	622915	624621	1	Poly(A+) RNA-binding protein	Poly(A+) RNA-binding protein; key surveillance factor for the selective export of spliced mRNAs from the nucleus to the cytoplasm; preference for intron-containing genes; similar to Npl3p; HRB1 has a paralog, GBP2, that arose from the whole genome duplication
S000004950	YNL005C	MRP7	Mitochondrial Ribosomal Protein	621313	622428	-1	Mitochondrial ribosomal protein of the large subunit	Mitochondrial ribosomal protein of the large subunit; involved together with Mrpl35p in assembly of cytochrome c oxidase
S000004951	YNL006W	LST8	Lethal with Sec Thirteen	620067	620978	1	Subunit of TORC1 and TORC2 involved in TOR signaling	Subunit of TORC1 and TORC2 involved in TOR signaling; TORC1 regulates nutrient responsive growth and TORC2 regulates plasma membrane (PM) homeostasis and cell-cycle dependent actin cytoskeleton polarization; negative regulator of Rtg1p/3p and Gln3p transcription factors and amino acid biosynthesis; binds to both Tor1p and Tor2p, regulating the integrity and kinase activity of TORC2; PM retention is dependent on Avo3p; WD-repeat protein
S000004952	YNL007C	SIS1	SIt4 Suppressor	618507	619565	-1	Type II HSP40 co-chaperone that interacts with the HSP70 protein Ssa1p	Type II HSP40 co-chaperone that interacts with the HSP70 protein Ssa1p; shuttles between cytosol and nucleus; mediates delivery of misfolded proteins into the nucleus for degradation; involved in proteasomal degradation of misfolded cytosolic proteins; protein abundance increases in response to DNA replication stress; polyQ aggregates sequester Sis1p and interfere with clearance of misfolded proteins; similar to bacterial DnaJ proteins and mammalian DnaJB1
S000004953	YNL008C	ASI3	Amino acid Sensor-Independent	616211	618241	-1	Subunit of the inner nuclear membrane Asi ubiquitin ligase complex	Subunit of the inner nuclear membrane Asi ubiquitin ligase complex; Asi complex targets both misfolded proteins of the inner nuclear membrane-associated degradation (INMAD) pathway and regulators of sterol biosynthesis for ubiquitin-mediated degradation; acts with Asi1p and Asi2p to ensure the fidelity of SPS-sensor signaling by targeting latent unprocessed forms of Stp1p and Stp2p, maintaining the repressed state of gene expression in the absence of inducing amino acids
S000004954	YNL009W	IDP3	Isocitrate Dehydrogenase, NADP-dependent	614821	616083	1	Peroxisomal NADP-dependent isocitrate dehydrogenase	Peroxisomal NADP-dependent isocitrate dehydrogenase; catalyzes oxidation of isocitrate to alpha-ketoglutarate with the formation of NADP(H+), required for growth on unsaturated fatty acids; IDP3 has a paralog, IDP2, that arose from the whole genome duplication
S000004955	YNL010W	PYP1	PolYol Phosphatase	613635	614360	1	Sugar alcohol phosphatase	Sugar alcohol phosphatase; polyol phosphatase that hydrolyzes sorbitol-6-phosphate, ribitol-5-phosphate, and (D)-glycerol-3-phosphate, maintaining phosphoglucose isomerase (PGI) activity in the presence of PGI-inhibitory sugar alcohols; expression correlated with growth rate; GFP-fusion protein localizes to the cytoplasm and nucleus; homozygous diploid mutant displays increased glycogen accumulation; member of the haloacid dehalogenase (HAD) superfamily 
S000004957	YNL012W	SPO1	SPOrulation	609686	611665	1	Meiosis-specific prospore protein	Meiosis-specific prospore protein; required for meiotic spindle pole body duplication and separation; required to produce bending force necessary for proper prospore membrane assembly during sporulation; has similarity to phospholipase B
S000004958	YNL013C	""	""	609133	609510	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF HEF3/YNL014W
S000004960	YNL015W	PBI2	Proteinase B Inhibitor	605384	605611	1	Cytosolic inhibitor of vacuolar proteinase B (PRB1)	Cytosolic inhibitor of vacuolar proteinase B (PRB1); required for efficient vacuole inheritance; with thioredoxin forms protein complex LMA1, which assists in priming SNARE molecules and promotes vacuole fusion; protein abundance increases in response to DNA replication stress
S000004961	YNL016W	PUB1	PolyUridylate Binding	602907	604268	1	Poly (A)+ RNA-binding protein	Poly (A)+ RNA-binding protein; abundant mRNP-component protein that binds cell wall mRNAs; required for stability of many mRNAs; component of glucose deprivation induced stress granules, involved in P-body-dependent granule assembly; implicated in regulation of translation; carries Q/N-rich domain at C- terminus, identified as candidate prion; ribosome-bound Pub1p modulates stop codon decoding during translation termination
S000004965	YNL020C	ARK1	Actin Regulating Kinase	595623	597539	-1	Ser/Thr protein kinase	Ser/Thr protein kinase; phosphorylates Pan1p-Sla1p-End3p protein complex subunits, Pan1p and Sla1p; involved in regulation of the cortical actin cytoskeleton and endocytosis; functional overlap with PRK1
S000004966	YNL021W	HDA1	Histone DeAcetylase	593227	595347	1	Catalytic subunit of class II HDA1 histone deacetylase complex	Catalytic subunit of class II HDA1 histone deacetylase complex; role in azole resistance via Hsp90p, and in heat shock response; Hda1p interacts with Hda2p-Hda3p subcomplex to form an active tetramer; deletion increases histone H2B, H3 and H4 acetylation; the HDA1 histone deacetylase complex regulates aging through trehalose metabolism
S000004967	YNL022C	RCM1	""	591427	592899	-1	rRNA m5C methyltransferase	rRNA m5C methyltransferase; methylates cytosine at position 2278 of 25S rRNA while Nop2p methylates cytosine at position 2870; contains seven beta-strand methyltransferase motif; localized to the nucleolus; interacts with Trm112p; homolog of NSUN5A, a human gene which is deleted in Williams-Beuren Syndrome
S000004968	YNL023C	FAP1	FKBP12-Associated Protein	588263	591160	-1	Protein that acts as sensor of stalled ribosomes	Protein that acts as sensor of stalled ribosomes; binds to Fpr1p and Smu2p, and triggers ubiquitination of stalled monosomes for subsequent degradation; confers rapamycin resistance by competing with rapamycin for Fpr1p binding; accumulates in the nucleus upon treatment of cells with rapamycin; has similarity to D. melanogaster shuttle craft and human NFX1
S000004969	YNL024C	EFM6	Elongation Factor Methyltransferase	587107	587847	-1	Putative S-adenosylmethionine-dependent lysine methyltransferase	Putative S-adenosylmethionine-dependent lysine methyltransferase; responsible for modifying Lys-390 in translational elongation factor EF-1 alpha (eEF1A); has seven beta-strand methyltransferase motif; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
S000004970	YNL025C	SSN8	Suppressor of SNf1	584320	585291	-1	Cyclin-like component of RNA polymerase II holoenzyme	Cyclin-like component of RNA polymerase II holoenzyme; involved in phosphorylation of RNAPII C-terminal domain; forms kinase-cyclin pair in RNAPII holoenzyme with Ssn3p; needed for entry into and execution of meiotic program; involved in glucose repression, telomere maintenance; degraded in response to nitrogen starvation, and also to oxidative stress, but in different cellular compartments, and with different cellular outcomes; 35% identical to human cyclin C
S000004971	YNL026W	SAM50	Sorting and Assembly Machinery	581920	583374	1	Component of the Sorting and Assembly Machinery (SAM) complex	Component of the Sorting and Assembly Machinery (SAM) complex; the SAM (or TOB) complex is located in the mitochondrial outer membrane; the complex binds precursors of beta-barrel proteins and facilitates their outer membrane insertion; homologous to bacterial Omp85
S000004972	YNL027W	CRZ1	Calcineurin-Responsive Zinc finger	579580	581616	1	Transcription factor, activates transcription of stress response genes	Transcription factor, activates transcription of stress response genes; nuclear localization is positively regulated by calcineurin-mediated dephosphorylation; rapidly localizes to the nucleus under blue light stress; can be activated in stochastic pulses of nuclear localization in response to calcium
S000004973	YNL028W	""	""	578679	578996	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000004974	YNL029C	KTR5	Kre Two Related	577205	578773	-1	Putative mannosyltransferase involved in protein glycosylation	Putative mannosyltransferase involved in protein glycosylation; member of the KRE2/MNT1 mannosyltransferase family; KTR5 has a paralog, KTR7, that arose from the whole genome duplication
S000004975	YNL030W	HHF2	Histone H Four	576727	577038	1	Histone H4	Histone H4; core histone protein required for chromatin assembly and chromosome function; one of two identical histone proteins (see also HHF1); contributes to telomeric silencing; N-terminal domain involved in maintaining genomic integrity
S000004976	YNL031C	HHT2	Histone H Three	575640	576050	-1	Histone H3	Histone H3; core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT1); regulated by acetylation, methylation, and phosphorylation; H3K14 acetylation plays an important role in the unfolding of strongly positioned nucleosomes during repair of UV damage
S000004977	YNL032W	SIW14	Synthetic Interaction with Whi2	574506	575351	1	Inositol phosphatase involved in inositol pyrophosphate metabolism	Inositol phosphatase involved in inositol pyrophosphate metabolism; hydrolyzes the beta-phosphate from the 5-diphosphate group of PP-InsPs including: 5PP-IP5 (IP7), 1,5PP-IP4 (IP8) and 5-PP-InsP4; functions as an anti-prion (anti-[PSI]) component; member of the atypical dual-specificity subgroup of the protein tyrosine-phosphatase (PTP) superfamily; localizes to the cytoplasm
S000004979	YNL034W	""	""	570477	572315	1	Protein required for sporulation	Protein required for sporulation; induced during meiosis and sporulation; gene is a member of Centroid, a centromere-linked gene family; not an essential gene; YNL034W has a paralog, YNL018C, that arose from a segmental duplication
S000004980	YNL035C	""	""	568522	569691	-1	Nuclear protein of unknown function	Nuclear protein of unknown function; relocalizes to the cytosol in response to hypoxia; contains WD-40 domains; not an essential gene; protein abundance increases in response to DNA replication stress
S000004982	YNL037C	IDH1	Isocitrate DeHydrogenase	557920	559002	-1	Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase	Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase; complex catalyzes the oxidation of isocitrate to alpha-ketoglutarate in the TCA cycle
S000004983	YNL038W	GPI15	GlycosylPhosphatidylInositol anchor biosynthesis	557020	557783	1	Protein involved in the synthesis of GlcNAc-PI	Protein involved in the synthesis of GlcNAc-PI; GlcNAc-PI is the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-H protein; GlcNAc-PI stands for N-acetylglucosaminyl phosphatidylinositol
S000004984	YNL039W	BDP1	B Double Prime	555048	556832	1	Essential subunit of RNA polymerase III transcription factor (TFIIIB)	Essential subunit of RNA polymerase III transcription factor (TFIIIB); TFIIIB is involved in transcription of genes encoding tRNAs, 5S rRNA, U6 snRNA, and other small RNAs
S000004985	YNL040W	""	""	553380	554750	1	Protein of unknown function	Protein of unknown function; has strong similarity to alanyl-tRNA synthases from Eubacteria; null mutant displays decreased translation rate and increased readthrough of premature stop codons; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL040W is not an essential gene
S000004986	YNL041C	COG6	Conserved Oligomeric Golgi complex	549468	551987	-1	Component of the conserved oligomeric Golgi complex	Component of the conserved oligomeric Golgi complex; a cytosolic tethering complex (Cog1p through Cog8p) that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
S000004988	YNL043C	""	""	545589	545909	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YIP3/YNL044W
S000004989	YNL044W	YIP3	Ypt-Interacting Protein	545268	545877	1	Protein localized to COPII vesicles	Protein localized to COPII vesicles; proposed to be involved in ER-to-Golgi transport; interacts with members of the Rab GTPase family and Yip1p; also interacts with Rtn1p; integral membrane protein that localizes to the ER, the Golgi apparatus, transport vesicles and the peroxisome; two isoforms exist, a full-length Yip3L (Yip3 Long) and a shorter peroxisomal matrix isoform Yip3S (Yip3 Short)
S000004990	YNL045W	LAP2	Leucine AminoPeptidases	542963	544978	1	Leucyl aminopeptidase yscIV with epoxide hydrolase activity	Leucyl aminopeptidase yscIV with epoxide hydrolase activity; metalloenzyme containing one zinc atom; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; also known as leukotriene A4 hydrolase
S000004991	YNL046W	""	""	542304	542822	1	Putative protein of unknown function	Putative protein of unknown function; expression depends on Swi5p; GFP-fusion protein localizes to the endoplasmic reticulum; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)
S000004992	YNL047C	SLM2	Synthetic Lethal with Mss4	539911	541881	-1	Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm1p	Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm1p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; TORC2 complex substrate and effector; SLM2 has a paralog, SLM1, that arose from the whole genome duplication
S000004994	YNL049C	SFB2	Sed Five Binding	535281	537911	-1	Component of the Sec23p-Sfb2p heterodimer of the COPII vesicle coat	Component of the Sec23p-Sfb2p heterodimer of the COPII vesicle coat; required for cargo selection during vesicle formation in ER to Golgi transport; homologous to Sfb3p; SFB2 has a paralog, SEC24, that arose from the whole genome duplication
S000004995	YNL050C	""	""	534079	534982	-1	Putative protein of unknown function	Putative protein of unknown function; YNL050c is not an essential gene
S000004996	YNL051W	COG5	Conserved Oligomeric Golgi complex	532659	533870	1	Component of the conserved oligomeric Golgi complex	Component of the conserved oligomeric Golgi complex; a cytosolic tethering complex (Cog1p through Cog8p) that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
S000004997	YNL052W	COX5A	Cytochrome c OXidase	531725	532186	1	Subunit Va of cytochrome c oxidase	Subunit Va of cytochrome c oxidase; cytochrome c oxidase is the terminal member of the mitochondrial inner membrane electron transport chain; Cox5Ap is predominantly expressed during aerobic growth while its isoform Vb (Cox5Bp) is expressed during anaerobic growth; COX5A has a paralog, COX5B, that arose from the whole genome duplication
S000004998	YNL053W	MSG5	Multicopy Suppressor of GPA1	529942	531411	1	Dual-specificity protein phosphatase	Dual-specificity protein phosphatase; maintains low levels of both basal and induced cell integrity pathway signaling by dephosphorylation of the Slt2p MAPK; reciprocally regulated by Slt2p through phosphorylation; minor role with Ptp2p in the adaptive response to pheromone through the dephosphorylation of the Fus3p MAPK with major contribution by Ptp3p; inhibits the nuclear accumulation of Fus3p; two isoforms exist as the result of alternative translation initiation starts
S000004999	YNL054W	VAC7	VACuolar segregation	526086	529583	1	Integral vacuolar membrane protein	Integral vacuolar membrane protein; involved in vacuole inheritance and morphology; activates Fab1p kinase activity under basal conditions and also after hyperosmotic shock; targeted to vacuole via AP-3 pathway
S000005000	YNL055C	POR1	PORin	517994	518845	-1	Mitochondrial porin (voltage-dependent anion channel)	Mitochondrial porin (voltage-dependent anion channel); outer membrane protein required for maintenance of mitochondrial osmotic stability and mitochondrial membrane permeability; couples the glutathione pools of the intermembrane space (IMS) and the cytosol; interacts with Om45 and Om14 in the outer membrane; phosphorylated; protein abundance increases in response to DNA replication stress
S000005001	YNL056W	OCA2	Oxidant-induced Cell cycle Arrest	517249	517842	1	Protein of unknown function	Protein of unknown function; similar to predicted tyrosine phosphatases Oca1p and Siw14p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YNL056W is not an essential gene
S000005002	YNL057W	""	""	516403	516735	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000005003	YNL058C	""	""	515763	516713	-1	Putative protein of unknown function	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to vacuole; not an essential gene; YNL058C has a paralog, PRM5, that arose from the whole genome duplication
S000005004	YNL059C	ARP5	Actin-Related Protein	512668	514935	-1	Nuclear actin-related protein involved in chromatin remodeling	Nuclear actin-related protein involved in chromatin remodeling; component of chromatin-remodeling enzyme complexes; promotes nucleosome shifts in the 3 prime direction
S000005005	YNL061W	NOP2	NucleOlar Protein	510540	512396	1	rRNA m5C methyltransferase	rRNA m5C methyltransferase; methylates cytosine at position 2870 of 25S rRNA; has an essential function independent of rRNA methylation; contains seven beta-strand methyltransferase motif; essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; localized to the nucleolus; constituent of 66S pre-ribosomal particles; rRNA methylation defect and lethality are functionally complemented by human NOP2, a gene upregulated in cancer
S000005006	YNL062C	GCD10	General Control Derepressed	508776	510212	-1	Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p	Subunit of tRNA (1-methyladenosine) methyltransferase with Gcd14p; required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression
S000005007	YNL063W	MTQ1	Methyltransferase	507758	508702	1	S-adenosylmethionine-dependent methyltransferase	S-adenosylmethionine-dependent methyltransferase; methylates translational release factor Mrf1p; similar to E.coli PrmC; is not an essential gene
S000005008	YNL064C	YDJ1	Yeast dnaJ	505868	507097	-1	Type I HSP40 co-chaperone	Type I HSP40 co-chaperone; involved in regulation of HSP90 and HSP70 functions; acts as an adaptor that helps Rsp5p recognize cytosolic misfolded proteins for ubiquitination after heat shock; critical for determining cell size at Start as a function of growth rate; involved in protein translocation across membranes; member of the DnaJ family; chimeric protein in which human p58IPK J domain replaces yeast Ydj1p J domain can complement yeast ydj1 mutant
S000005009	YNL065W	AQR1	Acids Quinidine Resistance	503724	505484	1	Plasma membrane transporter of the major facilitator superfamily	Plasma membrane transporter of the major facilitator superfamily; involved in the excretion of excess amino acids; member of the 12-spanner drug:H(+) antiporter DHA1 family; confers resistance to short-chain monocarboxylic acids and quinidine; relocalizes from plasma membrane to cytoplasm upon DNA replication stress; AQR1 has a paralog, QDR1, that arose from the whole genome duplication
S000005010	YNL066W	SUN4	Sim1 Uth1 Nca3	501515	502777	1	Cell wall protein related to glucanases localized in birth scars	Cell wall protein related to glucanases localized in birth scars; possibly involved in cell wall septation; member of the SUN family; SUN4 has a paralog, SIM1, that arose from the whole genome duplication
S000005011	YNL067W	RPL9B	Ribosomal Protein of the Large subunit	499681	500256	1	Ribosomal 60S subunit protein L9B	Ribosomal 60S subunit protein L9B; homologous to mammalian ribosomal protein L9 and bacterial L6; RPL9B has a paralog, RPL9A, that arose from a single-locus duplication
S000005012	YNL068C	FKH2	ForK head Homolog	495701	498289	-1	Forkhead family transcription factor	Forkhead family transcription factor; rate-limiting activator of replication origins; evolutionarily conserved regulator of lifespan; binds multiple chromosomal elements with distinct specificities, cell cycle dynamics; positively regulates transcriptional elongation; facilitates clustering, activation of early-firing replication origins; negative role in chromatin silencing at HML and HMR; major role in expression of G2/M phase genes; relocalizes to cytosol under hypoxia
S000005013	YNL069C	RPL16B	Ribosomal Protein of the Large subunit	493956	495001	-1	Ribosomal 60S subunit protein L16B	Ribosomal 60S subunit protein L16B; N-terminally acetylated, binds 5.8 S rRNA; transcriptionally regulated by Rap1p; homologous to mammalian ribosomal protein L13A and bacterial L13; RPL16B has a paralog, RPL16A, that arose from the whole genome duplication
S000005014	YNL070W	TOM7	Translocase of the Outer Mitochondrial membrane	493366	493548	1	Component of the TOM (translocase of outer membrane) complex	Component of the TOM (translocase of outer membrane) complex; responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex
S000005015	YNL071W	LAT1	""	491523	492971	1	Dihydrolipoamide acetyltransferase component (E2) of the PDC	Dihydrolipoamide acetyltransferase component (E2) of the PDC; the pyruvate dehydrogenase complex (PDC) catalyzes the oxidative decarboxylation of pyruvate to acetyl-CoA; metabolic longevity factor required for calorie restriction-mediated life span extension
S000005016	YNL072W	RNH201	RNase H	490317	491240	1	Ribonuclease H2 catalytic subunit	Ribonuclease H2 catalytic subunit; removes RNA primers during Okazaki fragment synthesis and errant ribonucleotides misincorporated during DNA replication; role in ribonucleotide excision repair; homolog of RNAse HI; related to human AGS4 which causes Aicardi-Goutieres syndrome
S000005017	YNL073W	MSK1	Mitochondrial aminoacyl-tRNA Synthetase, lysine (K)	488386	490116	1	Mitochondrial lysine-tRNA synthetase	Mitochondrial lysine-tRNA synthetase; required for import of both aminoacylated and deacylated forms of tRNA(Lys) into mitochondria and for aminoacylation of mitochondrially encoded tRNA(Lys)
S000005018	YNL074C	MLF3	Multicopy suppressor of LeFlunomide sensitivity	486766	488124	-1	Serine-rich protein of unknown function	Serine-rich protein of unknown function; predicted to be palmitoylated; overproduction suppresses growth inhibition caused by exposure to immunosuppressant leflunomide; MLF3 has a paralog, VHS2, that arose from the whole genome duplication
S000005019	YNL075W	IMP4	Interacting with Mpp10p	485607	486479	1	Component of the SSU processome	Component of the SSU processome; SSU processome is required for pre-18S rRNA processing; interacts with Mpp10p; member of a superfamily of proteins that contain a sigma(70)-like motif and associate with RNAs
S000005020	YNL076W	MKS1	Multicopy Kinase Suppressor	483556	485310	1	Pleiotropic negative transcriptional regulator	Pleiotropic negative transcriptional regulator; involved in Ras-CAMP and lysine biosynthetic pathways and nitrogen regulation; involved in retrograde (RTG) mitochondria-to-nucleus signaling
S000005021	YNL077W	APJ1	Anti-Prion DnaJ	481391	482977	1	Hsp40 chaperone with a role in SUMO-mediated protein degradation	Hsp40 chaperone with a role in SUMO-mediated protein degradation; works in concert with Hsp70 and Hsp110 (Sse1p) to promote disaggregation of intranuclear protein inclusions; competes with Hsp104 in disaggregation, supporting turnover instead of refolding; member of DnaJ-like family, conserved across eukaryotes; overexpression interferes with propagation of the [Psi+] prion; forms nuclear foci upon DNA replication stress
S000005022	YNL078W	NIS1	Neck protein Interacting with Septins	479768	480991	1	Protein involved in axial bud site selection	Protein involved in axial bud site selection; prevents repolarization of cells at previous division sites; recruited with Nba1p to the cell cortex and to cytokinesis remnants (bud scars) by Aim44p and Nap1p where Nba1p prevents Rsr1p-mediated activation of Cdc24p, and therefore Cdc42p activation; acts with Nba1p to establish transient bud scar localization of Rga1p, a Cdc42p GAP; localizes to the nucleus and to cell division site during G2/M, and is then stably recruited to cytokinesis remnants
S000005023	YNL079C	TPM1	TroPoMyosin	478566	479165	-1	Major isoform of tropomyosin	Major isoform of tropomyosin; binds to and stabilizes actin cables and filaments, which direct polarized cell growth and the distribution of several organelles; acetylated by the NatB complex and acetylated form binds actin most efficiently; TPM1 has a paralog, TPM2, that arose from the whole genome duplication
S000005024	YNL080C	EOS1	ER-localized and Oxidants Sensitive	476932	478032	-1	Protein involved in N-glycosylation	Protein involved in N-glycosylation; deletion mutation confers sensitivity to exidative stress and shows synthetic lethality with mutations in the spindle checkpoint genes BUB3 and MAD1; YNL080C is not an essential gene
S000005025	YNL081C	SWS2	Sick Without Securin	476188	476619	-1	Component of mitochondrial small ribosomal subunit	Component of mitochondrial small ribosomal subunit; similar to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency; localizes to vacuole in response to H2O2; localizes to peroxisomes in glucose-growing cells
S000005026	YNL082W	PMS1	PostMeiotic Segregation	473391	476012	1	ATP-binding protein required for mismatch repair	ATP-binding protein required for mismatch repair; required for both mitosis and meiosis; functions as a heterodimer with Mlh1p; binds double- and single-stranded DNA via its N-terminal domain; required for silencing at the silent mating-type loci and telomeres; similar to E. coli MutL
S000005027	YNL083W	SAL1	Suppressor of Aac2 Lethality	471377	472861	1	ADP/ATP transporter	ADP/ATP transporter; member of the Ca2+-binding subfamily of mitochondrial carriers, with two EF-hand motifs; transport activity of either Sal1p or Pet9p is critical for viability; polymorphic in different S. cerevisiae strains
S000005028	YNL084C	END3	ENDocytosis defective	470053	471102	-1	EH domain-containing protein involved in endocytosis	EH domain-containing protein involved in endocytosis; actin cytoskeletal organization and cell wall morphogenesis; forms a complex with Sla1p and Pan1p
S000005029	YNL085W	MKT1	Maintenance of K2 Killer Toxin	467131	469623	1	Protein similar to nucleases that forms a complex with Pbp1p	Protein similar to nucleases that forms a complex with Pbp1p; complex may mediate posttranscriptional regulation of HO; involved in propagation of M2 dsRNA satellite of L-A virus; allelic variation affects mitochondrial genome stability, drug resistance, and more; forms cytoplasmic foci upon DNA replication stress; localization to P-bodies under ethanol stress differs between strains
S000005030	YNL086W	SNN1	SNapiN	466334	466642	1	Subunit of the BLOC-1 complex involved in endosomal maturation	Subunit of the BLOC-1 complex involved in endosomal maturation; interacts with Msb3p; green fluorescent protein (GFP)-fusion protein localizes to endosomes
S000005031	YNL087W	TCB2	Three Calcium and lipid Binding domains (TriCalBins)	462411	465947	1	Lipid-binding ER tricalbin involved in ER-plasma membrane tethering	Lipid-binding ER tricalbin involved in ER-plasma membrane tethering; one of at least 7 proteins (Ice2p, Ist2p, Scs2/Scs22p, Tcb1-Tcb3p) that affect cER tethering and contact with the plasma membrane (PM); regulates PI4P levels by controlling access of Sac1p to its substrate PI4P in the PM; regulates PM phospholipid homeostasis; contains 3 calcium and lipid binding domains; localizes to the cortical ER, the cell periphery and is enriched in bud membranes; mRNA is targeted to the bud tip
S000005032	YNL088W	TOP2	TOPoisomerase	457704	461990	1	Topoisomerase II	Topoisomerase II; relieves torsional strain in DNA by cleaving and re-sealing phosphodiester backbone of both positively and negatively supercoiled DNA; cleaves complementary strands; localizes to axial cores in meiosis; required for replication slow zone (RSZ) breakage following Mec1p inactivation; human homolog TOP2A implicated in cancers, and can complement yeast null mutant
S000005033	YNL089C	""	""	456693	457169	-1	Dubious open reading frame unlikely to encode a functional protein	Dubious open reading frame unlikely to encode a functional protein; almost completely overlaps YNL090W/RHO2 which encodes a small GTPase of the Rho/Rac subfamily of Ras-like proteins
S000005034	YNL090W	RHO2	Ras HOmolog	456565	457143	1	Non-essential small GTPase of the Rho/Rac family of Ras-like proteins	Non-essential small GTPase of the Rho/Rac family of Ras-like proteins; involved in the establishment of cell polarity and in microtubule assembly
S000005035	YNL091W	NST1	Negatively affects Salt Tolerance	452408	456130	1	Translational inhibitor and suppressor of proteasome-assembly defects	Translational inhibitor and suppressor of proteasome-assembly defects; P-body component that inhibits translation, rescues proteasome base assembly defects and aggregation of regulatory particle subunits during stress when overexpressed; proposed to function to bridge the HOG and pheromone pathways with the cell wall integrity pathway in response to heat stress; interacts physically and genetically with splicing factor MSL1; large, highly-charge protein containing many disordered regions
S000005036	YNL092W	""	""	450871	452073	1	S-adenosylmethionine-dependent protein methyltransferase	S-adenosylmethionine-dependent protein methyltransferase; capable of automethylation; member of the seven beta-strand family; YNL092W is not an essential gene
S000005037	YNL093W	YPT53	Yeast Protein Two	449868	450530	1	Stress-induced Rab family GTPase	Stress-induced Rab family GTPase; required for vacuolar protein sorting and endocytosis; involved in ionic stress tolerance; similar to Vps21p and Ypt52p; functional homolog of Vps21p; mammalian Rab5 homolog; YPT53 has a paralog, VPS21, that arose from the whole genome duplication
S000005038	YNL094W	APP1	Actin Patch Protein	447611	449374	1	Phosphatidate phosphatase, converts phosphatidate to diacylglycerol	Phosphatidate phosphatase, converts phosphatidate to diacylglycerol; App1p, Pah1p, Dpp1p, and Lpp1p are responsible for all the phosphatidate phosphatase activity; component of cortical actin patches; interacts with components of endocytic pathway
S000005039	YNL095C	""	""	444912	446840	-1	Putative protein of unknown function	Putative protein of unknown function; predicted to contain a transmembrane domain; not an essential gene; YNL095C has a paralog, ECM3, that arose from the whole genome duplication
S000005040	YNL096C	RPS7B	Ribosomal Protein of the Small subunit	443398	444315	-1	Protein component of the small (40S) ribosomal subunit	Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7B has a paralog, RPS7A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
S000005041	YNL097C	PHO23	PHOsphate metabolism	441366	442358	-1	Component of the Rpd3L histone deacetylase complex	Component of the Rpd3L histone deacetylase complex; involved in transcriptional regulation of PHO5; affects termination of snoRNAs and cryptic unstable transcripts (CUTs); C-terminus shares significant sequence identity with the human candidate tumor suppressor p33-ING1 and its isoform ING3
S000005042	YNL098C	RAS2	homologous to RAS proto-oncogene	439602	440570	-1	GTP-binding protein	GTP-binding protein; regulates nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; activated by increased levels of glycolysis intermediate fructose-1,6-bisphosphate; homolog of mammalian Ras proto-oncogenes; RAS2 has a paralog, RAS1, that arose from the whole genome duplication
S000005043	YNL099C	OCA1	Oxidant-induced Cell-cycle Arrest	438567	439283	-1	Putative protein tyrosine phosphatase	Putative protein tyrosine phosphatase; required for cell cycle arrest in response to oxidative damage of DNA
S000005044	YNL100W	MIC27	MItochondrial contact site and Cristae organizing system	437613	438317	1	Component of the MICOS complex	Component of the MICOS complex; MICOS (formerly MINOS or MitOS), a mitochondrial inner membrane complex that extends into the intermembrane space with a role in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane; forms a subcomplex with Mic10p and Mic12p whose assembly and stability requires cardiolipin; orthologous to human APOO and APOOL
S000005045	YNL101W	AVT4	Amino acid Vacuolar Transport	434999	437140	1	Vacuolar transporter	Vacuolar transporter; exports large neutral amino acids from the vacuole; member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters; localizes to sites of contact between the vacuole and mitochondria (vCLAMPs)
S000005046	YNL102W	POL1	POLymerase	430087	434493	1	Catalytic subunit of the DNA polymerase I alpha-primase complex	Catalytic subunit of the DNA polymerase I alpha-primase complex; required for the initiation of DNA replication during mitotic DNA synthesis and premeiotic DNA synthesis
S000005047	YNL103W	MET4	METhionine requiring	427735	429753	1	Leucine-zipper transcriptional activator	Leucine-zipper transcriptional activator; responsible for regulation of sulfur amino acid pathway; requires different combinations of auxiliary factors Cbf1p, Met28p, Met31p and Met32p; feedforward loop exists in the regulation of genes controlled by Met4p and Met32p; can be ubiquitinated by ubiquitin ligase SCF-Met30p, is either degraded or maintained in an inactive state; regulates degradation of its own DNA-binding cofactors by targeting them to SCF-Met30p
S000005048	YNL104C	LEU4	LEUcine biosynthesis	424895	426754	-1	Alpha-isopropylmalate synthase (2-isopropylmalate synthase)	Alpha-isopropylmalate synthase (2-isopropylmalate synthase); the main isozyme responsible for the first step in the leucine biosynthesis pathway; LEU4 has a paralog, LEU9, that arose from the whole genome duplication
S000005049	YNL105W	RRT16	Regulator of rDNA Transcription	424155	424583	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified gene INP52; identified in a screen for mutants with decreased levels of rDNA transcription
S000005050	YNL106C	INP52	INositol polyphosphate 5-Phosphatase	420944	424495	-1	Polyphosphatidylinositol phosphatase	Polyphosphatidylinositol phosphatase; dephosphorylates a number of phosphatidylinositol phosphates (PtdInsPs, PIPs) to PI; involved in endocytosis; hyperosmotic stress causes translocation to actin patches; synaptojanin-like protein with a Sac1 domain; INP52 has a paralog, INP53, that arose from the whole genome duplication
S000005051	YNL107W	YAF9	Yeast homolog of the human leukemogenic protein AF9	420098	420778	1	Subunit of NuA4 histone H4 acetyltransferase and SWR1 complexes	Subunit of NuA4 histone H4 acetyltransferase and SWR1 complexes; may function to antagonize silencing near telomeres; interacts directly with Swc4p; has homology to human leukemogenic protein AF9; contains a YEATS domain
S000005052	YNL108C	""	""	419014	419826	-1	Protein phosphatase	Protein phosphatase; similar to prokaryotic phosphotransfer enzymes; null mutant shows alterations in glucose metabolism; GFP-fusion protein localizes to the cytoplasm and nucleus; YNL108C has a paralog, TFC7, that arose from the whole genome duplication
S000005053	YNL109W	""	""	418964	419509	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YNL108C
S000005054	YNL110C	NOP15	NucleOlar Protein	417826	418488	-1	Constituent of 66S pre-ribosomal particles	Constituent of 66S pre-ribosomal particles; involved in 60S ribosomal subunit biogenesis; localizes to both nucleolus and cytoplasm
S000005055	YNL111C	CYB5	CYtochrome B	416940	417302	-1	Cytochrome b5	Cytochrome b5; involved in the sterol and lipid biosynthesis pathways; acts as an electron donor to support sterol C5-6 desaturation
S000005056	YNL112W	DBP2	Dead Box Protein	413639	416281	1	ATP-dependent RNA helicase of the DEAD-box protein family	ATP-dependent RNA helicase of the DEAD-box protein family; has strong preference for dsRNA; remodels RNA-protein complex (RNP) to facilitate efficient transcription termination ; required for assembly of Yra1p, Nab2p and Mex67p onto mRNA and formation of nuclear mRNP; involved in mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites
S000005057	YNL113W	RPC19	RNA Polymerase C	412771	413199	1	RNA polymerase subunit AC19	RNA polymerase subunit AC19; common to RNA polymerases I and III
S000005058	YNL114C	""	""	412684	413055	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF RPC19/YNL113W, an RNA polymerase subunit
S000005059	YNL115C	""	""	410119	412053	-1	Putative protein of unknown function	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL115C is not an essential gene
S000005060	YNL116W	DMA2	Defective in Mitotic Arrest	408341	409909	1	Ubiquitin-protein ligase (E3)	Ubiquitin-protein ligase (E3); controls septin dynamics and spindle position checkpoint (SPOC) with ligase Dma1p by regulating recruitment of Elm1p to bud neck; regulates levels of eIF2 subunit Gcd11p, as well as abundance, localization, and ubiquitination of Cdk inhibitory kinase Swe1p; ortholog of human RNF8, similar to human Chfr; contains FHA and RING finger domains; DMA2 has a paralog, DMA1, that arose from the whole genome duplication
S000005061	YNL117W	MLS1	MaLate Synthase	406358	408022	1	Malate synthase, enzyme of the glyoxylate cycle	Malate synthase, enzyme of the glyoxylate cycle; involved in utilization of non-fermentable carbon sources; expression is subject to carbon catabolite repression; localizes in peroxisomes during growth on oleic acid, otherwise cytosolic; can accept butyryl-CoA as acyl-CoA donor in addition to traditional substrate acetyl-CoA
S000005063	YNL119W	NCS2	Needs Cla4 to Survive	401040	402521	1	Protein required for uridine thiolation of Lys(UUU) and Glu(UUC) tRNAs	Protein required for uridine thiolation of Lys(UUU) and Glu(UUC) tRNAs; required for the thiolation of uridine at the wobble position of Lys(UUU) and Glu(UUC) tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae
S000005064	YNL120C	""	""	401033	401518	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion enhances replication of Brome mosaic virus in S. cerevisiae, but likely due to effects on the overlapping gene
S000005065	YNL121C	TOM70	Translocase of the Outer Mitochondrial membrane	398684	400537	-1	Component of the TOM (translocase of outer membrane) complex	Component of the TOM (translocase of outer membrane) complex; involved in the recognition and initial import steps for all mitochondrially-directed proteins, acting as a receptor for incoming precursor proteins; moonlighting role as a transcriptional regulator of mitochondrial proteins; age-dependent reduction in Tom70p results in mitochondrial dysfunction, a hallmark of aging; localizes to mitochondria–ER contact sites; TOM70 has a paralog, TOM71, that arose from the whole genome duplication 
S000005066	YNL122C	MRP35	Mitochondrial Ribosomal Protein	398023	398370	-1	Mitochondrial ribosomal protein of the large subunit	Mitochondrial ribosomal protein of the large subunit; homologous to bacterial L35 and human MRPL35 ribosomal proteins
S000005067	YNL123W	NMA111	Nuclear Mediator of Apoptosis	394685	397678	1	Serine protease and general molecular chaperone	Serine protease and general molecular chaperone; cleaves Roq1p, which modifies the substrate specificity of the Ubr1p Ub-ligase, promoting the stress-induced homeostatically-regulated protein degradation (SHRED) of misfolded and native ER-membrane and cytosolic proteins; chaperone activity involved in the heat stress response; promotes apoptosis through proteolysis of Bir1p; role in lipid homeostasis; mammalian Omi/HtrA2 serine protease family member
S000005068	YNL124W	NAF1	Nuclear Assembly Factor	392892	394370	1	RNA-binding protein required for the assembly of box H/ACA snoRNPs	RNA-binding protein required for the assembly of box H/ACA snoRNPs; thus required for pre-rRNA processing; forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p
S000005069	YNL125C	ESBP6	""	390146	392167	-1	Protein with similarity to monocarboxylate permeases	Protein with similarity to monocarboxylate permeases; appears not to be involved in transport of monocarboxylates such as lactate, pyruvate or acetate across the plasma membrane
S000005071	YNL127W	FAR11	Factor ARrest	383987	386848	1	Protein involved in recovery from cell cycle arrest	Protein involved in recovery from cell cycle arrest; acts in response to pheromone; also involved in regulation of intra-S DNA damage checkpoint and autophagy; is essential for dephosphorylation of Atg13p; interacts with Far3p, Far7p, Far8p, Far9p, Far10p and with the phosphatases Pph21p, Pph22p and Pph3p; has similarity to the N- and C-termini of N. crassa HAM-2; similar to human Fam40A and Fam40B
S000005072	YNL128W	TEP1	TEnsin-like Phosphatase	382359	383663	1	PTEN homolog with no demonstrated inositol lipid phosphatase activity	PTEN homolog with no demonstrated inositol lipid phosphatase activity; plays a role in normal sporulation; homolog of human tumor suppressor gene PTEN/MMAC1/TEP1 and fission yeast ptn1
S000005073	YNL129W	NRK1	Nicotinamide Riboside Kinase	381480	382202	1	Nicotinamide riboside kinase	Nicotinamide riboside kinase; catalyzes the phosphorylation of nicotinamide riboside and nicotinic acid riboside in salvage pathways for NAD+ biosynthesis
S000005074	YNL130C	CPT1	CholinePhosphoTransferase	379558	380831	-1	Cholinephosphotransferase	Cholinephosphotransferase; required for phosphatidylcholine biosynthesis and for inositol-dependent regulation of EPT1 transcription; CPT1 has a paralog, EPT1, that arose from the whole genome duplication
S000005075	YNL131W	TOM22	Translocase of the Outer Mitochondrial membrane	378767	379225	1	Component of the TOM (Translocase of Outer Membrane) complex	Component of the TOM (Translocase of Outer Membrane) complex; responsible for initial import of mitochondrially directed proteins; mediates interaction between TOM and TIM complexes and acts as receptor for precursor proteins; implicated in mitochondrial accumulation of amyloid beta peptides via direct interaction with amyloid beta peptide residues 25-42 followed by transfer to Tom40p and transport through the TOM channel
S000005076	YNL132W	KRE33	Killer toxin REsistant	375321	378491	1	Acetyltransferase required for biogenesis of small ribosomal subunit	Acetyltransferase required for biogenesis of small ribosomal subunit; conserved 18S rRNA cytosine acetyltransferase that modifies tRNAs assisted by adaptor Tan1p; responsible for incorporation of N4-acetylcytidine into mRNAs; human homolog NAT10 implicated in several types of cancer and premature aging
S000005077	YNL133C	FYV6	Function required for Yeast Viability	374171	374692	-1	Protein of unknown function	Protein of unknown function; required for survival upon exposure to K1 killer toxin; proposed to regulate double-strand break repair via non-homologous end-joining
S000005078	YNL134C	""	""	372451	373581	-1	NADH-dependent aldehyde reductase	NADH-dependent aldehyde reductase; involved in detoxification of furfural; expression is up-regulated in the presence of furfural and 5-hydroxymethylfurfural, which are compounds generated during lignocellulosic biomass pre-treatment; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress
S000005079	YNL135C	FPR1	Fk 506-sensitive Proline Rotamase	371882	372226	-1	Peptidyl-prolyl cis-trans isomerase (PPIase)	Peptidyl-prolyl cis-trans isomerase (PPIase); binds to FK506 and rapamycin; also binds to the nonhistone chromatin binding protein Hmo1p and may regulate its assembly or function; associates with promoters of ribosomal protein genes; acts as chaperone to prevent protein aggregation; N-terminally propionylated in vivo; mutation is functionally complemented by human FKBP1A
S000005080	YNL136W	EAF7	Esa1-Associated Factor	370368	371645	1	Subunit of the NuA4 histone acetyltransferase complex	Subunit of the NuA4 histone acetyltransferase complex; NuA4 acetylates the N-terminal tails of histones H4 and H2A
S000005081	YNL137C	NAM9	Nuclear Accommodation of Mitochondria	368595	370055	-1	Mitochondrial ribosomal component of the small subunit	Mitochondrial ribosomal component of the small subunit
S000005082	YNL138W	SRV2	Suppressor of RasVal19	366741	368321	1	CAP (cyclase-associated protein)	CAP (cyclase-associated protein); N-terminus binds adenylate cyclase and facilitates activation by RAS; N-terminus forms novel hexameric star-shaped shuriken structures that directly catalyze cofilin-mediated severing of actin filaments; C-terminus binds and recycles cofilin bound, ADP-actin monomers, facilitating regulation of actin dynamics and cell morphogenesis; N- and C-termini can function as physically separate proteins; mCAP1 is the mouse homolog
S000005083	YNL139C	THO2	suppressor of the Transcriptional defect of Hpr1 by Overexpression	360924	365717	-1	Subunit of the THO complex	Subunit of the THO complex; THO is required for efficient transcription elongation and involved in transcriptional elongation-associated recombination; required for LacZ RNA expression from certain plasmids
S000005084	YNL140C	""	""	360917	361486	-1	Protein of unknown function	Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps THO2/YNL139C
S000005085	YNL141W	AAH1	Adenine AminoHydrolase	359596	360639	1	Adenine deaminase (adenine aminohydrolase)	Adenine deaminase (adenine aminohydrolase); converts adenine to hypoxanthine; involved in purine salvage; transcriptionally regulated by nutrient levels and growth phase; Aah1p degraded upon entry into quiescence via SCF and the proteasome
S000005086	YNL142W	MEP2	""	357453	358952	1	Ammonium permease involved in regulation of pseudohyphal growth	Ammonium permease involved in regulation of pseudohyphal growth; belongs to Mep-Amt-Rh family of well-conserved ammonium (NH4+) transporters that includes human Rh factors; expression is under nitrogen catabolite repression regulation; activity is controlled by phospho-silencing; phosphorylation of Mep2 mediated by Npr1; dephosphorylation involves Psr1p and Psr2p; Twin-His motif ensures transport specificity
S000005087	YNL143C	""	""	356794	357186	-1	Protein of unknown function	Protein of unknown function; expressed at both mRNA and protein levels
S000005088	YNL144C	""	""	352820	355042	-1	Putative protein of unknown function	Putative protein of unknown function; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; contains a PH domain and binds phosphatidylinositols and phosphatidylethanolamine in a large-scale study; YNL144C has a paralog, YHR131C, that arose from the whole genome duplication
S000005089	YNL145W	MFA2	Mating Factor A	352414	352530	1	Mating pheromone a-factor	Mating pheromone a-factor; made by a cells; interacts with alpha cells to induce cell cycle arrest and other responses leading to mating; biogenesis involves C-terminal modification, N-terminal proteolysis, and export; also encoded by MFA1
S000005090	YNL146W	""	""	351715	352017	1	Putative protein of unknown function	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNL146W is not an essential gene
S000005091	YNL147W	LSM7	Like SM	350940	351383	1	Lsm (Like Sm) protein	Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress
S000005092	YNL148C	ALF1	ALpha tubulin Folding	349907	350671	-1	Alpha-tubulin folding protein	Alpha-tubulin folding protein; similar to mammalian cofactor B; Alf1p-GFP localizes to cytoplasmic microtubules; required for the folding of alpha-tubulin and may play an additional role in microtubule maintenance
S000005095	YNL151C	RPC31	RNA Polymerase C	347766	348521	-1	RNA polymerase III subunit C31	RNA polymerase III subunit C31
S000005097	YNL153C	GIM3	Gene Involved in Microtubule biogenesis	345669	346058	-1	Subunit of the heterohexameric cochaperone prefoldin complex	Subunit of the heterohexameric cochaperone prefoldin complex; prefoldin binds specifically to cytosolic chaperonin and transfers target proteins to it; prefoldin complex also localizes to chromatin of actively transcribed genes in the nucleus and facilitates transcriptional elongation
S000005098	YNL154C	YCK2	Yeast Casein Kinase	343636	345276	-1	Palmitoylated plasma membrane-bound casein kinase I (CK1) isoform	Palmitoylated plasma membrane-bound casein kinase I (CK1) isoform; shares redundant functions with Yck1p in morphogenesis, proper septin assembly, endocytic trafficking, and glucose sensing; stabilized by Sod1p binding in the presence of glucose and oxygen, causing glucose repression of respiratory metabolism; involved in the phosphorylation and regulation of glucose sensor Rgt2p; YCK2 has a paralog, YCK1, that arose from the whole genome duplication
S000005099	YNL155W	CUZ1	Cdc48-associated UBL/Zn-finger protein	342516	343340	1	Protein with a role in the ubiquitin-proteasome pathway	Protein with a role in the ubiquitin-proteasome pathway; interacts with ubiquitinated protein, Cdc48p and the proteasomal regulatory particle; may protect cells from trivalent metalloid induced proteotoxicity; contains a PACE promoter element and is co-regulated with proteasome subunit genes; AN1-type zinc finger protein, with DHHC and ubiquitin-like domains (UBL); ortholog of ZFAND1, a human gene linked to cancer; protein abundance increases under DNA replication stress
S000005100	YNL156C	NSG2	""	341069	341968	-1	Protein involved in regulation of sterol biosynthesis	Protein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyze the rate-limiting step in sterol biosynthesis; homolog of mammalian INSIG proteins; NSG2 has a paralog, NSG1, that arose from the whole genome duplication
S000005101	YNL157W	IGO1	Initiation of G zerO	340352	340858	1	Protein required for initiation of G0 program	Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; IGO1 has a paralog, IGO2, that arose from the whole genome duplication
S000005102	YNL158W	PGA1	Processing of Gas1p and ALP	339612	340208	1	Essential component of GPI-mannosyltransferase II	Essential component of GPI-mannosyltransferase II; complex is responsible for second mannose addition to GPI precursors as a partner of Gpi18p; required for maturation of Gas1p and Pho8p; has synthetic genetic interactions with secretory pathway genes
S000005103	YNL159C	ASI2	Amino acid Sensor-Independent	338478	339347	-1	Subunit of the inner nuclear membrane Asi ubiquitin ligase complex	Subunit of the inner nuclear membrane Asi ubiquitin ligase complex; the Asi complex targets both misfolded proteins of the inner nuclear membrane-associated degradation (INMAD) pathway and inner for ubiquitin-mediated degradation; acts with Asi1p and Asi3p to ensure the fidelity of SPS-sensor signaling by targeting latent unprocessed forms of Stp1p and Stp2p, maintaining the repressed state of gene expression in the absence of inducing amino acids
S000005104	YNL160W	YGP1	Yeast GlycoProtein	336545	337609	1	Cell wall-related secretory glycoprotein	Cell wall-related secretory glycoprotein; induced by nutrient deprivation-associated growth arrest and upon entry into stationary phase; may be involved in adaptation prior to stationary phase entry; protein demonstrates amyloid properties in vivo; YGP1 has a paralog, SPS100, that arose from the whole genome duplication
S000005105	YNL161W	CBK1	Cell wall Biosynthesis Kinase	332597	334867	1	Serine/threonine protein kinase of the the RAM signaling network	Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress
S000005106	YNL162W	RPL42A	Ribosomal Protein of the Large subunit	331322	332154	1	Ribosomal 60S subunit protein L42A	Ribosomal 60S subunit protein L42A; homologous to mammalian ribosomal protein L36A, no bacterial homolog; RPL42A has a paralog, RPL42B, that arose from the whole genome duplication
S000005107	YNL163C	RIA1	RIbosome Assembly	326741	330073	-1	Cytoplasmic GTPase/eEF2-like factor involved in ribosomal biogenesis	Cytoplasmic GTPase/eEF2-like factor involved in ribosomal biogenesis; with Sdo1p, a guanine nucleotide exchange factor (GEF), promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; required for quality control check of newly made large ribosomal subunits before they are released into the pool of translating ribosomes
S000005108	YNL164C	IBD2	Inhibition of Bud Division	325265	326320	-1	Component of the BUB2-dependent spindle checkpoint pathway	Component of the BUB2-dependent spindle checkpoint pathway; interacts with Bfa1p and functions upstream of Bub2p and Bfa1p
S000005109	YNL165W	""	""	323832	325052	1	Putative protein of unknown function	Putative protein of unknown function; YNL165W is not an essential gene
S000005110	YNL166C	BNI5	Bud Neck Involved	322219	323565	-1	Linker protein responsible for recruitment of myosin to the bud neck	Linker protein responsible for recruitment of myosin to the bud neck; interacts with the C-terminal extensions of septins Cdc11p and Shs1p and binds Myo1p to promote cytokinesis
S000005111	YNL167C	SKO1	Suppressor of Kinase Overexpression	319416	321359	-1	Basic leucine zipper transcription factor of the ATF/CREB family	Basic leucine zipper transcription factor of the ATF/CREB family; forms a complex with Tup1p and Cyc8p to both activate and repress transcription; cytosolic and nuclear protein involved in osmotic and oxidative stress responses
S000005112	YNL168C	FMP41	Found in Mitochondrial Proteome	318030	318809	-1	Putative protein of unknown function	Putative protein of unknown function; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
S000005113	YNL169C	PSD1	PhosphatidylSerine Decarboxylase	316169	317671	-1	Phosphatidylserine decarboxylase of the mitochondrial inner membrane	Phosphatidylserine decarboxylase of the mitochondrial inner membrane; converts phosphatidylserine to phosphatidylethanolamine; regulates mitochondrial fusion and morphology; partly exposed to the mitochondrial intermembrane space; ER-localized fraction is involved in formation of lipid droplets; autocatalytically processed
S000005114	YNL170W	""	""	315978	316373	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000005115	YNL171C	""	""	315629	315997	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000005116	YNL172W	APC1	Anaphase Promoting Complex subunit	310636	315882	1	Largest subunit of the Anaphase-Promoting Complex/Cyclosome	Largest subunit of the Anaphase-Promoting Complex/Cyclosome; APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; localizes to nuclear foci that become diffuse upon DNA replication stress
S000005117	YNL173C	MDG1	Multicopy suppressor of Defective G-protein	308957	310057	-1	Plasma membrane protein	Plasma membrane protein; involved in G-protein mediated pheromone signaling pathway; overproduction suppresses bem1 mutations; MDG1 has a paralog, CRP1, that arose from the whole genome duplication
S000005119	YNL175C	NOP13	NucleOlar Protein	307401	308612	-1	Nucleolar protein found in preribosomal complexes	Nucleolar protein found in preribosomal complexes; contains an RNA recognition motif (RRM); relative distribution to the nucleolus increases upon DNA replication stress
S000005120	YNL176C	TDA7	Topoisomerase I Damage Affected	305070	306980	-1	Cell cycle-regulated gene of unknown function	Cell cycle-regulated gene of unknown function; promoter bound by Fkh2p; null mutant is sensitive to expression of the top1-T722A allele; TDA7 has a paralog, YDL211C, that arose from the whole genome duplication
S000005121	YNL177C	MRPL22	Mitochondrial Ribosomal Protein, Large subunit	303686	304615	-1	Mitochondrial ribosomal protein of the large subunit	Mitochondrial ribosomal protein of the large subunit
S000005123	YNL179C	""	""	300666	301103	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; deletion in cyr1 mutant results in loss of stress resistance
S000005125	YNL181W	PBR1	Potentiates Bioactive compound Response	298335	299558	1	Putative oxidoreductase	Putative oxidoreductase; required for cell viability
S000005126	YNL182C	IPI3	Involved in Processing ITS2	295960	297627	-1	Component of the Rix1 complex and pre-replicative complexes (pre-RCs)	Component of the Rix1 complex and pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; highly conserved protein which contains several WD40 motifs; IPI3 is an essential gene; other members include Rix1p, Ipi1p, and Ipi3p
S000005127	YNL183C	NPR1	Nitrogen Permease Reactivator	293137	295509	-1	Protein kinase	Protein kinase; stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis by phosphorylating Ldb19p; positively regulates activity of the three Mep ammonium transport proteins; mediates inhibitory phosphorylation of Mep2p and Par32p; TOR complex negatively regulates Npr1p activity; NPR1 has a paralog, PRR2, that arose from the whole genome duplication
S000005128	YNL184C	""	""	292557	292883	-1	Protein of unknown function	Protein of unknown function; expressed at both mRNA and protein levels
S000005131	YNL187W	SWT21	Synthetic With Tgs1	287994	289067	1	Protein involved in mRNA splicing	Protein involved in mRNA splicing; contains a consensus nuclear export signal (NES) sequence similar to the consensus sequence recognized by Crm1p; interacts genetically with Prp40p and Tgs1p; contains WD40 repeats
S000005132	YNL188W	KAR1	KARyogamy	286308	287609	1	Protein involved in karyogamy and spindle pole body duplication	Protein involved in karyogamy and spindle pole body duplication; involved in karyogamy during mating; involved in centrosome separation and duplication during mitosis; localizes to the half-bridge of the spindle pole body; interacts with Spc72p during karyogamy; also interacts with Cdc31p; essential gene
S000005133	YNL189W	SRP1	Suppressor of Rna Polymerase I	284260	285888	1	Karyopherin alpha homolog	Karyopherin alpha homolog; forms a dimer with karyopherin beta Kap95p to mediate import of nuclear proteins, binds the nuclear localization signal of the substrate during import; involved in cotranslational protein degradation; binds ribosome-bound nascent polypeptides; Srp1p and Sts1p couple proteasomes to nascent polypeptides emerging from the ribosome for cotranslational degradation
S000005134	YNL190W	""	""	282395	283009	1	Hydrophilin essential in desiccation-rehydration process	Hydrophilin essential in desiccation-rehydration process; cell wall protein; contains a putative GPI-attachment site
S000005135	YNL191W	DUG3	Deficient in Utilization of Glutathione	280432	281505	1	Component of glutamine amidotransferase (GATase II)	Component of glutamine amidotransferase (GATase II); forms a complex with Dug2p to degrade glutathione (GSH) and other peptides containing a gamma-glu-X bond in an alternative pathway to GSH degradation by gamma-glutamyl transpeptidase (Ecm38p)
S000005136	YNL192W	CHS1	CHitin Synthase	276502	279897	1	Chitin synthase I	Chitin synthase I; requires activation from zymogenic form in order to catalyze the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for repairing the chitin septum during cytokinesis; transcription activated by mating factor
S000005137	YNL193W	""	""	274368	276044	1	Putative protein of unknown function	Putative protein of unknown function; exhibits a two-hybrid interaction with Yhr151cp in a large-scale analysis
S000005138	YNL194C	""	""	272710	273615	-1	Integral membrane protein	Integral membrane protein; required for sporulation and plasma membrane sphingolipid content; similar to SUR7; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; GFP-fusion protein is more abundant at MCCs (membrane compartment occupied by Can1) in the presence of glycerol and oleate; YNL194C has a paralog, FMP45, that arose from the whole genome duplication
S000005139	YNL195C	""	""	271523	272308	-1	Protein of unknown function	Protein of unknown function; shares a promoter with YNL194C; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YNL195C has a paralog, HBT1, that arose from the whole genome duplication
S000005140	YNL196C	SLZ1	""	270275	271171	-1	Sporulation-specific protein with a leucine zipper motif	Sporulation-specific protein with a leucine zipper motif; subunit of the MIS complex which controls mRNA methylation during during the induction of sporulation
S000005141	YNL197C	WHI3	WHIskey	267608	269593	-1	RNA binding protein that modulates mRNA stability	RNA binding protein that modulates mRNA stability; regulates cell cycle, sister chromatid cohesion, and stress response genes; modulates stability and translational efficiency of CLN3 mRNA; colocalizes with P-bodies/stress granules to regulate target mRNAs; regulates cell fate and critical cell size for Start passage; regulates ploidy; self-templating mnemon that forms inactive super-assemblies, preventing G1 arrest in mother cells after unsuccessful mating and causing sterility in old cells
S000005142	YNL198C	""	""	266514	266816	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000005143	YNL199C	GCR2	GlyColysis Regulation	264926	266530	-1	Transcriptional activator of genes involved in glycolysis	Transcriptional activator of genes involved in glycolysis; interacts and functions with the DNA-binding protein Gcr1p
S000005144	YNL200C	NNR1	Nicotinamide Nucleotide Repair	263713	264453	-1	NADHX epimerase	NADHX epimerase; catalyzes isomerization of (R)- and (S)-NADHX; homologous to AIBP in mammals and the N- terminal domain of YjeF in E.coli; enzyme is widespread in eukaryotes, prokaryotes and archaea; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
S000005145	YNL201C	PSY2	Platinum SensitivitY	260627	263203	-1	Subunit of protein phosphatase PP4 complex	Subunit of protein phosphatase PP4 complex; Pph3p and Psy2p form the active complex, Psy4p may provide additional substrate specificity; regulates recovery from the DNA damage checkpoint, the gene conversion- and single-strand annealing-mediated pathways of meiotic double-strand break repair and efficient Non-Homologous End-Joining (NHEJ) pathway; Pph3p and Psy2p localize to foci on meiotic chromosomes; putative homolog of mammalian R3
S000005146	YNL202W	SPS19	SPorulation-Specific	259578	260456	1	Peroxisomal 2,4-dienoyl-CoA reductase	Peroxisomal 2,4-dienoyl-CoA reductase; auxiliary enzyme of fatty acid beta-oxidation; homodimeric enzyme required for growth and sporulation on petroselineate medium; expression induced during late sporulation and in the presence of oleate
S000005147	YNL203C	""	""	259439	260050	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000005148	YNL204C	SPS18	SPorulation-Specific	258375	259277	-1	Protein of unknown function, contains a putative zinc-binding domain	Protein of unknown function, contains a putative zinc-binding domain; expressed during sporulation; SPS18 has a paralog, GCS1, that arose from the whole genome duplication
S000005149	YNL205C	""	""	258155	258577	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000005150	YNL206C	RTT106	Regulator of Ty1 Transposition	256788	258155	-1	Histone chaperone	Histone chaperone; involved in regulation of chromatin structure in both transcribed and silenced chromosomal regions; affects transcriptional elongation; has a role in regulation of Ty1 transposition; interacts physically and functionally with Chromatin Assembly Factor-1 (CAF-1)
S000005151	YNL207W	RIO2	RIght Open reading frame	255353	256630	1	Essential serine kinase involved in the processing of 20S pre-rRNA	Essential serine kinase involved in the processing of 20S pre-rRNA; involved in the processing of the 20S pre-rRNA into mature 18S rRNA; has similarity to Rio1p
S000005152	YNL208W	""	""	254418	255017	1	Protein of unknown function	Protein of unknown function; may interact with ribosomes, based on co-purification experiments; authentic, non-tagged protein is detected in purified mitochondria in high-throughput studies; potential orthologs found in other fungi
S000005153	YNL209W	SSB2	Stress-Seventy subfamily B	252059	253900	1	Cytoplasmic ATPase that is a ribosome-associated molecular chaperone	Cytoplasmic ATPase that is a ribosome-associated molecular chaperone; functions with J-protein partner Zuo1p; may be involved in the folding of newly-synthesized polypeptide chains; member of the HSP70 family; SSB2 has a paralog, SSB1, that arose from the whole genome duplication
S000005155	YNL211C	MRX7	Mitochondrial oRganization of gene eXpression (MIOREX)	250055	250315	-1	Protein that associates with mitochondrial ribosome	Protein that associates with mitochondrial ribosome; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL211C is not an essential gene
S000005156	YNL212W	VID27	Vacuolar Import and Degradation	247461	249809	1	Cytoplasmic protein of unknown function	Cytoplasmic protein of unknown function; possibly involved in vacuolar protein degradation; not essential for proteasome-dependent degradation of fructose-1,6-bisphosphatase (FBPase); null mutants exhibit normal growth; contains two PH-like domains
S000005157	YNL213C	RRG9	Required for Respiratory Growth	246460	247104	-1	Protein of unknown function	Protein of unknown function; null mutant lacks mitochondrial DNA and cannot grow on glycerol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
S000005158	YNL214W	PEX17	PEroXisome related	245617	246216	1	Membrane peroxin of the peroxisomal importomer complex	Membrane peroxin of the peroxisomal importomer complex; complex facilitates the import of peroxisomal matrix proteins; required for peroxisome biogenesis
S000005159	YNL215W	IES2	Ino Eighty Subunit	244468	245430	1	Protein that associates with the INO80 chromatin remodeling complex	Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress
S000005160	YNL216W	RAP1	Repressor/Activator site binding Protein	241689	244172	1	Essential DNA-binding transcription regulator that binds many loci	Essential DNA-binding transcription regulator that binds many loci; involved in transcription activation, repression, chromatin silencing, telomere length maintenance; represses divergent noncoding transcription at highly expressed genes, prevents transcription initiation at cryptic promoters near its binding sites; relocalizes to cytosol under hypoxia; recruits Sir complex to telomeric DNA; present in quiescent cell telomere hyperclusters
S000005161	YNL217W	PPN2	""	240331	241311	1	Zn2+-dependent endopolyphosphatase	Zn2+-dependent endopolyphosphatase; required with PPN1 to mobilize polyphosphate stores in response to phosphate starvation; member of the PPP-superfamily of metalloproteases; localizes to the vacuolar lumen via the MVB pathway; null mutant is highly sensitive to azaserine and resistant to sodium-O-vandate
S000005162	YNL218W	MGS1	Maintenance of Genome Stability	238238	240001	1	Protein with DNA-dependent ATPase and ssDNA annealing activities	Protein with DNA-dependent ATPase and ssDNA annealing activities; involved in maintenance of genome; interacts functionally with DNA polymerase delta; homolog of human Werner helicase interacting protein (WHIP); forms nuclear foci upon DNA replication stress
S000005163	YNL219C	ALG9	Asparagine-Linked Glycosylation	235996	237663	-1	Mannosyltransferase, involved in N-linked glycosylation	Mannosyltransferase, involved in N-linked glycosylation; catalyzes the transfer of both the seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; human homolog ALG9 can complement yeast null mutant; mutation of human homolog causes type 1 congenital disorders of glycosylation
S000005164	YNL220W	ADE12	ADEnine requiring	234413	235714	1	Adenylosuccinate synthase	Adenylosuccinate synthase; catalyzes the first step in synthesis of adenosine monophosphate from inosine 5'monophosphate during purine nucleotide biosynthesis; exhibits binding to single-stranded autonomously replicating (ARS) core sequence
S000005165	YNL221C	POP1	Processing Of Precursor RNAs	231068	233695	-1	Subunit of RNase MRP, nuclear RNase P and telomerase complexes	Subunit of RNase MRP, nuclear RNase P and telomerase complexes; RNase MRP cleaves pre-rRNA, nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs, while telomerase replenishes telomeric DNA; binds to the RPR1 RNA subunit in RNase P
S000005166	YNL222W	SSU72	Suppressor of SUa7, gene 2	229094	229714	1	Phosphatase and transcription/RNA-processing factor	Phosphatase and transcription/RNA-processing factor; associates with TFIIB and cleavage/polyadenylation factor Pta1p; exhibits phosphatase activity on serine-5 and serine-7 of the RNA polymerase II C-terminal domain; affects start site selection and transcriptional read through in vivo
S000005167	YNL223W	ATG4	AuTophaGy related	227370	228854	1	Conserved cysteine protease required for autophagy	Conserved cysteine protease required for autophagy; cleaves Atg8p to a form required for autophagosome and Cvt vesicle generation
S000005168	YNL224C	SQS1	SQuelch of Splicing suppression	224796	227099	-1	Protein that stimulates the ATPase and helicase activities of Prp43p	Protein that stimulates the ATPase and helicase activities of Prp43p; acts with Prp43p to stimulate 18s rRNA maturation by Nob1p; overexpression antagonizes the suppression of splicing defects by spp382 mutants; component of pre-ribosomal particles; relocalizes from nucleus to nucleolus upon DNA replication stress
S000005169	YNL225C	CNM67	Chaotic Nuclear Migration	222724	224469	-1	Component of the spindle pole body outer plaque	Component of the spindle pole body outer plaque; required for spindle orientation and mitotic nuclear migration; CNM67 has a paralog, ADY3, that arose from the whole genome duplication
S000005170	YNL226W	""	""	222239	222649	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene JJJ1/YNL227C
S000005171	YNL227C	JJJ1	J-protein (Type III)	220659	222431	-1	Co-chaperone that stimulates the ATPase activity of Ssa1p	Co-chaperone that stimulates the ATPase activity of Ssa1p; required for a late step of ribosome biogenesis; associated with the cytosolic large ribosomal subunit; contains a J-domain; mutation causes defects in fluid-phase endocytosis
S000005172	YNL228W	""	""	220645	221421	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF YNL227C/JJJ1
S000005173	YNL229C	URE2	UREidosuccinate transport	219137	220201	-1	Nitrogen catabolite repression transcriptional regulator	Nitrogen catabolite repression transcriptional regulator; inhibits GLN3 transcription in good nitrogen source; role in sequestering Gln3p and Gat1p to the cytoplasm; has glutathione peroxidase activity and can mutate to acquire GST activity; self-assembly under limited nitrogen conditions creates [URE3] prion and releases catabolite repression
S000005174	YNL230C	ELA1	ELongin A	217523	218662	-1	Elongin A	Elongin A; F-box protein that forms a heterodimer with Elc1p and is required for ubiquitin-dependent degradation of the RNA Polymerase II subunit Rpo21p; subunit of the Elongin-Cullin-Socs (ECS) ligase complex
S000005175	YNL231C	PDR16	Pleiotropic Drug Resistance	215987	217042	-1	Phosphatidylinositol transfer protein (PITP)	Phosphatidylinositol transfer protein (PITP); controlled by the multiple drug resistance regulator Pdr1p; localizes to lipid particles and microsomes; controls levels of various lipids, may regulate lipid synthesis; homologous to Pdr17p; protein abundance increases in response to DNA replication stress
S000005176	YNL232W	CSL4	Cep1 Synthetic Lethal	214923	215801	1	Exosome non-catalytic core component	Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; predicted to contain an S1 RNA binding domain; human homolog EXOSC1 partially complements yeast csl4 null mutant, and can complement inviability of strain in which expression of CSL4 is repressed
S000005177	YNL233W	BNI4	Bud Neck Involved	211922	214600	1	Targeting subunit for Glc7p protein phosphatase	Targeting subunit for Glc7p protein phosphatase; localized to the bud neck, required for localization of chitin synthase III to the bud neck via interaction with the chitin synthase III regulatory subunit Skt5p; phosphorylation by Slt2p and Kss1p involved in regulating Bni4p in septum assembly
S000005178	YNL234W	""	""	210233	211513	1	Protein of unknown function with similarity to globins	Protein of unknown function with similarity to globins; has a functional heme-binding domain; mutant has aneuploidy tolerance; transcription induced by stress conditions; may be involved in glucose signaling or metabolism; regulated by Rgt1
S000005179	YNL235C	""	""	209548	209979	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SIN4/YNL236W, a subunit of the mediator complex
S000005180	YNL236W	SIN4	Switch INdependent	206930	209854	1	Subunit of the RNA polymerase II mediator complex	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both positive and negative transcriptional regulation; dispensible for basal transcription
S000005181	YNL237W	YTP1	Yeast putative Transmembrane Protein	205188	206567	1	Probable type-III integral membrane protein of unknown function	Probable type-III integral membrane protein of unknown function; has regions of similarity to mitochondrial electron transport proteins
S000005182	YNL238W	KEX2	Killer EXpression defective	202428	204872	1	Kexin, a subtilisin-like protease (proprotein convertase)	Kexin, a subtilisin-like protease (proprotein convertase); a calcium-dependent serine protease involved in the activation of proproteins of the secretory pathway
S000005183	YNL239W	LAP3	Leucine AminoPeptidases	200569	201933	1	Cysteine aminopeptidase with homocysteine-thiolactonase activity	Cysteine aminopeptidase with homocysteine-thiolactonase activity; protects cells against homocysteine toxicity; cytosolic protein, selectively transported to vacuole during nitrogen starvation; targeted to vacuole via CVT pathway; has bleomycin hydrolase activity in vitro; transcription is regulated by galactose via Gal4p; orthologous to human BLMH
S000005184	YNL240C	NAR1	Nuclear Architecture Related	198502	199977	-1	Subunit of the cytosolic iron-sulfur (FeS) protein assembly machinery	Subunit of the cytosolic iron-sulfur (FeS) protein assembly machinery; required for maturation of cytosolic and nuclear FeS proteins and for normal resistance to oxidative stress; deficiency results in shortened lifespan and sensitivity to paraquat; homologous to human Narf
S000005185	YNL241C	ZWF1	ZWischenFerment	196426	197943	-1	Glucose-6-phosphate dehydrogenase (G6PD)	Glucose-6-phosphate dehydrogenase (G6PD); catalyzes the first step of the pentose phosphate pathway; involved in adaptation to oxidative stress; negative regulator of nonselective autophagy, independent of Stb5p; protein abundance increases in response to DNA replication stress; homolog of human G6PD which is deficient in patients with hemolytic anemia; human G6PD can complement yeast zwf1 null mutant
S000005186	YNL242W	ATG2	AuTophaGy related	191324	196102	1	Putative lipid transport protein	Putative lipid transport protein; peripheral membrane protein required for vesicle formation during autophagy, pexophagy and the CVT pathway; directed to autophagic membranes by Atg18p where it acts as a PAS-ER membrane tether during autophagosome formation; involved in Atg9p cycling between the PAS and mitochondria; binds the phospholipid PI(3)P via an APT1 domain; essential for cell cycle progression from G2/M to G1 under nitrogen starvation; forms cytoplasmic foci upon DNA replication stress
S000005188	YNL244C	SUI1	SUppressor of Initiator codon	187170	187496	-1	Translation initiation factor eIF1	Translation initiation factor eIF1; component of complex involved in recognition of initiator codon; modulates translation accuracy at initiation phase by discriminating against suboptimal initiation sites to prevent excessive uORF translation genome-wide
S000005189	YNL245C	CWC25	Complexed With Cef1p	186346	186885	-1	Splicing factor required for the first step of pre-mRNA splicing	Splicing factor required for the first step of pre-mRNA splicing; binding to the spliceosome requires Prp2p and Yju2p; heat-stable protein; has similarity to S. pombe Cwf25p
S000005190	YNL246W	VPS75	Vacuolar Protein Sorting	185460	186349	1	NAP family histone chaperone	NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair; protein abundance increases in response to DNA replication stress; relocalizes to the cytosol in response to hypoxia
S000005191	YNL247W	CRS1	Cysteinyl-tRNA Synthetase, CysRS	182875	185178	1	Cysteinyl-tRNA synthetase	Cysteinyl-tRNA synthetase; CRS1 has two in-frame start codons resulting in a shorter isoform that is retained in the cytosol and a longer form that is mitochondrial; may interact with ribosomes, based on co-purification experiments; human gene CARS allows growth of the yeast haploid null mutant after sporulation of a heterozygous diploid
S000005192	YNL248C	RPA49	RNA Polymerase A	181361	182608	-1	RNA polymerase I subunit A49	RNA polymerase I subunit A49; essential for nucleolar assembly and for high polymerase loading rate; required for nucleolar localization of Rpa34p
S000005193	YNL249C	MPA43	Multicopy PDC1 Activator	179394	181022	-1	Protein of unknown function	Protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
S000005194	YNL250W	RAD50	RADiation sensitive	175410	179348	1	Subunit of MRX complex with Mre11p and Xrs2p	Subunit of MRX complex with Mre11p and Xrs2p; complex is involved in processing double-strand DNA breaks in vegetative cells, initiation of meiotic DSBs, telomere maintenance, and nonhomologous end joining; forms nuclear foci upon DNA replication stress
S000005195	YNL251C	NRD1	Nuclear pre-mRNA Down-regulation	172588	174315	-1	RNA-binding subunit of Nrd1 complex	RNA-binding subunit of Nrd1 complex; complex interacts with exosome to mediate 3'-end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; interacts with CTD of RNA pol II large subunit Rpo21p at phosphorylated Ser5 to direct transcription termination of non-polyadenylated transcripts; H3K4 trimethylation of transcribed regions by Set1p enhances recruitment of Nrd1p to those sites; role in regulation of mitochondrial abundance and cell size
S000005196	YNL252C	MRPL17	Mitochondrial Ribosomal Protein, Large subunit	171441	172286	-1	Mitochondrial ribosomal protein of the large subunit	Mitochondrial ribosomal protein of the large subunit
S000005197	YNL253W	TEX1	TrEX component	170018	171286	1	Protein involved in mRNA export	Protein involved in mRNA export; component of the transcription export (TREX) complex
S000005198	YNL254C	RTC4	Restriction of Telomere Capping	168043	169248	-1	Protein of unknown function	Protein of unknown function; null mutation suppresses cdc13-1 temperature sensitivity; (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
S000005199	YNL255C	GIS2	GIg Suppressor	167329	167790	-1	Translational activator for mRNAs with internal ribosome entry sites	Translational activator for mRNAs with internal ribosome entry sites; associates with polysomes and binds to a specific subset of mRNAs; localizes to RNA processing bodies (P bodies) and to stress granules; may have a role in translation regulation under stress conditions; ortholog of human ZNF9/CNBP, a gene involved in myotonic dystrophy type 2
S000005200	YNL256W	FOL1	FOLic acid synthesis	164623	167097	1	Multifunctional enzyme of the folic acid biosynthesis pathway	Multifunctional enzyme of the folic acid biosynthesis pathway; has dihydropteroate synthetase, dihydro-6-hydroxymethylpterin pyrophosphokinase, and dihydroneopterin aldolase activities
S000005201	YNL257C	SIP3	SNF1-Interacting Protein	160631	164320	-1	Putative sterol transfer protein	Putative sterol transfer protein; has a probable role in retrograde transport of sterols from the plasma membrane to the ER; co-localizes to puncta in the cortical ER with Ysp2p; contains GRAM, StART-like (VASt) and two PH-like domains; one of 6 StART-like domain-containing proteins in yeast that may be involved in sterol transfer between intracellular membranes; conserved across eukaryotes; previously identified as a transcription cofactor that interacts with DNA-bound Snf1p
S000005202	YNL258C	DSL1	Dependence on SLy1-20	158109	160373	-1	Peripheral membrane protein needed for Golgi-to-ER retrograde traffic	Peripheral membrane protein needed for Golgi-to-ER retrograde traffic; mediates Sey1p-independent homotypic ER fusion; forms Dsl1 tethering complex with Sec39p and Tip20p that forms a stable complex with ER SNAREs Sec20p, Ufe1p and Use1p and is functionally conserved from yeast to mammalian cells; component of the ER target site that interacts with coatomer; interacts with different subunits of COPI vesicle coat; interacts with Cin5p; homolog of fly and human ZW10 gene
S000005203	YNL259C	ATX1	AnTioXidant	157644	157865	-1	Cytosolic copper metallochaperone	Cytosolic copper metallochaperone; transports copper to the secretory vesicle copper transporter Ccc2p for eventual insertion into Fet3p, which is a multicopper oxidase required for high-affinity iron uptake; human homolog ATOX1 can complement yeast atx1 mutant; overexpression of human ATOX1 suppresses lysine auxotrophy of the yeast sod1 null mutant, as does overexpression of yeast ATX1
S000005204	YNL260C	LTO1	required for biogenesis of the Large ribosomal subunit and initiation of Translation in Oxygen	156859	157347	-1	Substrate-specific adaptor protein involved in apo-Rli1p maturation	Substrate-specific adaptor protein involved in apo-Rli1p maturation; subunit of the Yae1-Lto1 complex, recruiting apo-Rli1p to the CIA targeting complex, facilitating the insertion of an Fe/S cluster by the Fe-S biosynthetic machinery; deca-GX3 motif crucial for complex formation; essential for aerobic but not anaerobic growth; homolog of human ORAOV1, which is overexpressed in solid tumors; co-expression of ORAOV1 and YAE1 homolog YAE1D1 restores growth and Rli1p maturation after LTO1 depletion
S000005205	YNL261W	ORC5	Origin Recognition Complex	155100	156539	1	Subunit of the origin recognition complex (ORC)	Subunit of the origin recognition complex (ORC); ORC directs DNA replication by binding to replication origins and is also involved in transcriptional silencing
S000005206	YNL262W	POL2	POLymerase	148212	154880	1	Catalytic subunit of DNA polymerase (II) epsilon	Catalytic subunit of DNA polymerase (II) epsilon; a chromosomal DNA replication polymerase that exhibits processivity and proofreading exonuclease activity; participates in leading-strand synthesis during DNA replication; also involved in DNA synthesis during DNA repair; interacts extensively with Mrc1p
S000005207	YNL263C	YIF1	YIP1-Interacting Factor	146896	147840	-1	Integral membrane protein	Integral membrane protein; required for the fusion of ER-derived COPII transport vesicles with the Golgi; interacts with Yip1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF3
S000005208	YNL264C	PDR17	Pleiotropic Drug Resistance	145563	146615	-1	Phosphatidylinositol transfer protein (PITP)	Phosphatidylinositol transfer protein (PITP); downregulates Plb1p-mediated turnover of phosphatidylcholine; forms a complex with Psd2p which appears essential for maintenance of vacuolar PE levels; found in the cytosol and microsomes; homologous to Pdr16p; deletion affects phospholipid composition
S000005209	YNL265C	IST1	Increased Sodium Tolerance	144280	145281	-1	Protein involved in recycling of membrane transporters from endosome t	Protein involved in recycling of membrane transporters from endosome to plasma membrane; plays a role in the multivesicular body sorting pathway; functions and forms a complex with Did2p; recruitment to endosomes is mediated by the Vps2p-Vps24p subcomplex of ESCRT-III; also interacts with Vps4p
S000005210	YNL266W	""	""	144245	144664	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF IST1/YNL265C
S000005211	YNL267W	PIK1	Phosphatidyl Inositol Kinase	140878	144078	1	Phosphatidylinositol 4-kinase	Phosphatidylinositol 4-kinase; catalyzes the first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton; required for autophagosome formation during autophagy and for lipophagy in both stationary phase cells and during nitrogen starvation; may control nonselective autophagy and mitophagy through trafficking of Atg9p; localizes to the nucleus and to the trans-Golgi network
S000005212	YNL268W	LYP1	LYsine-specific Permease	138550	140385	1	Lysine permease	Lysine permease; one of three amino acid permeases (Alp1p, Can1p, Lyp1p) responsible for uptake of cationic amino acids
S000005213	YNL269W	BSC4	Bypass of Stop Codon	137699	138094	1	Protein of unknown function	Protein of unknown function; protein-coding gene that evolved de novo via a series of point mutations in noncoding sequence; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough is increased upon depletion of Sup35p; may be involved in DNA repair pathway during stationary phase and contribute to robustness of cells when shifted to a nutrient-poor environment
S000005214	YNL270C	ALP1	""	135940	137661	-1	Arginine transporter	Arginine transporter; expression is normally very low and it is unclear what conditions would induce significant expression; ALP1 has a paralog, CAN1, that arose from the whole genome duplication
S000005215	YNL271C	BNI1	Bud Neck Involved	129522	135383	-1	Formin	Formin; polarisome component; nucleates the formation of linear actin filaments, involved in cell processes such as budding and mitotic spindle orientation which require the formation of polarized actin cables; recruited to the division site in a Glc7p/Ref2p dependent manner following release of Bnr1p; functionally redundant with BNR1
S000005216	YNL272C	SEC2	SECretory	126805	129084	-1	Guanyl-nucleotide exchange factor for the small G-protein Sec4p	Guanyl-nucleotide exchange factor for the small G-protein Sec4p; essential for post-Golgi vesicle transport and for autophagy; associates with the exocyst, via exocyst subunit Sec15p, on secretory vesicles
S000005217	YNL273W	TOF1	TOpoisomerase I-interacting Factor	122883	126599	1	Subunit of a replication-pausing checkpoint complex	Subunit of a replication-pausing checkpoint complex; Tof1p-Mrc1p-Csm3p acts at the stalled replication fork to promote sister chromatid cohesion after DNA damage, facilitating gap repair of damaged DNA; interacts with the MCM helicase; relocalizes to the cytosol in response to hypoxia
S000005218	YNL274C	GOR1	GlyOxylate Reductase	121118	122170	-1	Glyoxylate reductase	Glyoxylate reductase; null mutation results in increased biomass after diauxic shift; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress
S000005219	YNL275W	BOR1	BORon transporter	119268	120998	1	Boron efflux transporter of the plasma membrane	Boron efflux transporter of the plasma membrane; binds HCO3-, I-, Br-, NO3- and Cl-; has similarity to the characterized boron efflux transporter A. thaliana BOR1
S000005220	YNL276C	""	""	118431	118826	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene MET2/YNL277W
S000005221	YNL277W	MET2	METhionine requiring	117349	118809	1	L-homoserine-O-acetyltransferase	L-homoserine-O-acetyltransferase; catalyzes the conversion of homoserine to O-acetyl homoserine which is the first step of the methionine biosynthetic pathway
S000005222	YNL278W	CAF120	CCR4 Associated Factor	113271	116453	1	Part of the CCR4-NOT transcriptional regulatory complex	Part of the CCR4-NOT transcriptional regulatory complex; involved in controlling mRNA initiation, elongation, and degradation; contains a PH-like domain; CAF120 has a paralog, SKG3, that arose from the whole genome duplication
S000005223	YNL279W	PRM1	Pheromone-Regulated Membrane protein	110917	112902	1	Pheromone-regulated multispanning membrane protein	Pheromone-regulated multispanning membrane protein; involved in membrane fusion during mating; predicted to have 5 transmembrane segments and a coiled coil domain; localizes to the shmoo tip; regulated by Ste12p
S000005224	YNL280C	ERG24	ERGosterol biosynthesis	109095	110411	-1	C-14 sterol reductase	C-14 sterol reductase; acts in ergosterol biosynthesis; mutants accumulate the abnormal sterol ignosterol (ergosta-8,14 dienol), and are viable under anaerobic growth conditions but inviable on rich medium under aerobic conditions
S000005225	YNL281W	HCH1	High-Copy Hsp90 suppressor	108467	108928	1	Heat shock protein regulator	Heat shock protein regulator; binds to Hsp90p and may stimulate ATPase activity; originally identified as a high-copy number suppressor of a HSP90 loss-of-function mutation; role in regulating Hsp90 inhibitor drug sensitivity; GFP-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress
S000005226	YNL282W	POP3	Processing Of Precursor RNAs	107687	108274	1	Subunit of both RNase MRP and nuclear RNase P	Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; increases the activity and thermal stability of the RNase P complex, along with Pop2p; relocalizes to the cytosol in response to hypoxia
S000005227	YNL283C	WSC2	cell Wall integrity and Stress response Component	105184	106695	-1	Sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway	Sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway; involved in maintenance of cell wall integrity and recovery from heat shock; required for the arrest of secretion response; WSC2 has a paralog, WSC3, that arose from the whole genome duplication
S000005228	YNL284C	MRPL10	Mitochondrial Ribosomal Protein, Large subunit	103134	104102	-1	Mitochondrial ribosomal protein of the large subunit	Mitochondrial ribosomal protein of the large subunit; appears as two protein spots (YmL10 and YmL18) on two-dimensional SDS gels
S000005229	YNL285W	""	""	96173	96544	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000005230	YNL286W	CUS2	Cold sensitive U2 snRNA Suppressor	95223	96080	1	Putative checkpoint factor in transcription	Putative checkpoint factor in transcription; binds to U2 snRNA and Prp11p; regulates toggling of the U2 snRNA stem II region between different structures; contains two RNA recognition motifs (RRMs)
S000005231	YNL287W	SEC21	SECretory	91994	94801	1	Gamma subunit of coatomer	Gamma subunit of coatomer; coatomer is a heptameric protein complex that together with Arf1p forms the COPI coat; involved in ER to Golgi transport of selective cargo
S000005232	YNL288W	CAF40	CCR4 Associated Factor	90303	91424	1	Component of the CCR4-NOT transcriptional complex	Component of the CCR4-NOT transcriptional complex; evolutionarily conserved; involved in controlling mRNA initiation, elongation, and degradation; binds Cdc39p
S000005233	YNL289W	PCL1	Pho85 CycLin	87897	88736	1	Cyclin, interacts with cyclin-dependent kinase Pho85p	Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; is ubiquitinated by Dma1p; phosphorylation by Pho85p targets it for degradation; localizes to sites of polarized cell growth
S000005234	YNL290W	RFC3	Replication Factor C	86218	87240	1	Subunit of heteropentameric Replication factor C (RF-C)	Subunit of heteropentameric Replication factor C (RF-C); which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon; relocalizes to the cytosol in response to hypoxia
S000005235	YNL291C	MID1	Mating pheromone-Induced Death	84169	85815	-1	Stretch-activated Ca2+-permeable cation channel	Stretch-activated Ca2+-permeable cation channel; required for Ca2+ influx stimulated by mating pheromones and some abiotic stresses; sensor of compressive mechanical stress; N-glycosylated membrane protein of the ER and plasma membrane; interacts with Cch1p; oligomerizes; is an alpha2/delta-like protein and participates in the generation of stretch-activated Ca2+-permeable cation channel activity when expressed in mammalian cells
S000005236	YNL292W	PUS4	PseudoUridine Synthase	82806	84017	1	Pseudouridine synthase	Pseudouridine synthase; catalyzes only the formation of pseudouridine-55 (Psi55), a highly conserved tRNA modification, in mitochondrial and cytoplasmic tRNAs; also responsible for pseudouracil modification of some mRNAs; PUS4 overexpression leads to translational derepression of GCN4 (Gcd- phenotype)
S000005237	YNL293W	MSB3	Multicopy Suppressor of Bud Emergence	80640	82541	1	Rab GTPase-activating protein	Rab GTPase-activating protein; regulates endocytosis via inactivation of Vps21p at endosomes and vacuole fusion via inactivation of Ypt7p at vacuoles; also acts on Ypt52p and Sec4p; localizes to plasma membrane, sites of polarized growth; relocalizes from bud neck to cytoplasm upon DNA replication stress; similar to TBC-domain Tre2 oncogene; MSB3 has a paralog, MSB4, that arose from the whole genome duplication; human homolog USP6NL can complement yeast msb3 msb4 double null
S000005238	YNL294C	RIM21	Regulator of IME2	78659	80260	-1	pH sensor molecule, component of RIM101 pathway	pH sensor molecule, component of RIM101 pathway; positive regulator of cell size; has role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH
S000005239	YNL295W	MRX6	Mitochondrial oRganization of gene eXpression (MIOREX)	76946	78520	1	Mitochondrial ribosome-associated protein	Mitochondrial ribosome-associated protein; regulates mitochondrial DNA copy number and the size and distribution of mtDNA nucleoids; subunit of a complex containing Pet20p, Mam33p, and Pim1p that may regulate mtDNA replication; member of the PET20 domain-containing protein family; forms foci that partially co-localize with mtDNA
S000005240	YNL296W	""	""	76273	76587	1	Dubious open reading frame unlikely to encode a functional protein	Dubious open reading frame unlikely to encode a functional protein; deletion adversely affects sporulation; deletion mutant exhibits synthetic phenotype under expression of mutant huntingtin fragment, but gene does not have human ortholog
S000005241	YNL297C	MON2	MONensin sensitivity	71673	76583	-1	Protein with a role in endocytosis and vacuole integrity	Protein with a role in endocytosis and vacuole integrity; peripheral membrane protein; interacts with and negatively regulates Arl1p; localizes to the endosome; member of the Sec7p family of proteins
S000005242	YNL298W	CLA4	CLn Activity dependant	68915	71443	1	Cdc42p-activated signal transducing kinase	Cdc42p-activated signal transducing kinase; member of the PAK (p21-activated kinase) family, along with Ste20p and Skm1p; involved in septin ring assembly, vacuole inheritance, cytokinesis, sterol uptake regulation; phosphorylates Cdc3p and Cdc10p; CLA4 has a paralog, SKM1, that arose from the whole genome duplication
S000005243	YNL299W	TRF5	Topoisomerase one-Related Function	66517	68445	1	Non-canonical poly(A) polymerase	Non-canonical poly(A) polymerase; involved in nuclear RNA degradation as a component of the TRAMP complex; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; overlapping but non-redundant functions with Pap2p
S000005244	YNL300W	TOS6	""	65744	66052	1	Glycosylphosphatidylinositol-dependent cell wall protein	Glycosylphosphatidylinositol-dependent cell wall protein; expression is periodic and decreases in respone to ergosterol perturbation or upon entry into stationary phase; depletion increases resistance to lactic acid
S000005245	YNL301C	RPL18B	Ribosomal Protein of the Large subunit	63570	64562	-1	Ribosomal 60S subunit protein L18B	Ribosomal 60S subunit protein L18B; homologous to mammalian ribosomal protein L18, no bacterial homolog; RPL18B has a paralog, RPL18A, that arose from the whole genome duplication
S000005246	YNL302C	RPS19B	Ribosomal Protein of the Small subunit	61958	62943	-1	Protein component of the small (40S) ribosomal subunit	Protein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19B has a paralog, RPS19A, that arose from the whole genome duplication
S000005247	YNL303W	""	""	61510	61857	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000005248	YNL304W	YPT11	Yeast Protein Two	60297	61550	1	Rab GTPase	Rab GTPase; Myo2p-binding protein implicated in mother-to-bud transport of cortical endoplasmic reticulum (ER), late Golgi, and mitochondria during cell division; function is regulated at multiple levels; abundance of active Ypt11p forms is controlled by phosphorylation status and degradation; normally a low-abundance protein whose ER localization is only detected when protein is highly over expressed
S000005249	YNL305C	BXI1	BaX Inhibitor	58898	59791	-1	Protein involved in apoptosis	Protein involved in apoptosis; variously described as containing a BCL-2 homology (BH3) domain or as a member of the BAX inhibitor family; reported to promote apoptosis under some conditions and to inhibit it in others; localizes to ER and vacuole; may link the unfolded protein response to apoptosis via regulation of calcium-mediated signaling; translocates to mitochondria under apoptosis-inducing conditions in a process involving Mir1p and Cor1p
S000005250	YNL306W	MRPS18	Mitochondrial Ribosomal Protein, Small subunit	58155	58808	1	Mitochondrial ribosomal protein of the small subunit	Mitochondrial ribosomal protein of the small subunit; essential for viability, unlike most other mitoribosomal proteins
S000005251	YNL307C	MCK1	Meiosis and Centromere regulatory Kinase	56446	57573	-1	Dual-specificity S/T and tyrosine protein kinase	Dual-specificity S/T and tyrosine protein kinase; spindle position checkpoint component that works in parallel with Kin4p to prevent mitotic exit network (MEN) activation by the Cdc14 early anaphase release (FEAR) network; roles in chromosome segregation, meiotic entry, genome stability, phosphorylation-dependent degradation of Rcn1p and Cdc6p, inhibition of protein kinase A, transcriptional regulation, inhibition of RNAPIII, calcium stress and inhibition of Clb2p-Cdc28p after nuclear division
S000005252	YNL308C	KRI1	KRR1-Interacting protein	54122	55897	-1	Essential nucleolar protein required for 40S ribosome biogenesis	Essential nucleolar protein required for 40S ribosome biogenesis; associate with snR30; physically and functionally interacts with Krr1p
S000005253	YNL309W	STB1	Sin Three Binding protein	52662	53924	1	Protein with role in regulation of MBF-specific transcription at Start	Protein with role in regulation of MBF-specific transcription at Start; phosphorylated by Cln-Cdc28p kinases in vitro; unphosphorylated form binds Swi6p, which is required for Stb1p function; expression is cell-cycle regulated; STB1 has a paralog, YOL131W, that arose from the whole genome duplication
S000005254	YNL310C	ZIM17	ZInc finger Motif protein of 17 kDa	51907	52431	-1	Protein co-chaperone with a zinc finger motif	Protein co-chaperone with a zinc finger motif; essential for protein import into mitochondria; may act with Pam18p to facilitate recognition and folding of imported proteins by Ssc1p (mtHSP70) in the mitochondrial matrix; required for the maintenance of Ssc1p solubility and assists in the functional interaction of Ssc1p with substrate proteins
S000005255	YNL311C	SKP2	homology with human SKP2	49397	51688	-1	F-box protein of unknown function	F-box protein of unknown function; predicted to be part of an SCF ubiquitin protease complex; involved in regulating protein levels of sulfur metabolism enzymes; may interact with ribosomes, based on co-purification experiments
S000005256	YNL312W	RFA2	Replication Factor A	48287	49216	1	Subunit of heterotrimeric Replication Protein A (RPA)	Subunit of heterotrimeric Replication Protein A (RPA); RPA is a highly conserved single-stranded DNA binding protein involved in DNA replication, repair, and recombination; RPA protects against inappropriate telomere recombination, and upon telomere uncapping, prevents cell proliferation by a checkpoint-independent pathway; in concert with Sgs1p-Top2p-Rmi1p, stimulates DNA catenation/decatenation activity of Top3p; protein abundance increases in response to DNA replication s
S000005257	YNL313C	EMW1	Essential for Maintenance of the cell Wall	45308	48022	-1	Essential conserved protein with a role in cell wall integrity	Essential conserved protein with a role in cell wall integrity; contains six TPR (tetratricopeptide repeat) domains clustered in the C-terminal region; conditional mutant is suppressed by overexpression of GFA1; protein abundance increases in response to DNA replication stress
S000005258	YNL314W	DAL82	Degradation of Allantoin	44447	45214	1	Positive regulator of allophanate inducible genes	Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain
S000005259	YNL315C	ATP11	ATP synthase	43324	44280	-1	Molecular chaperone	Molecular chaperone; required for the assembly of alpha and beta subunits into the F1 sector of mitochondrial F1F0 ATP synthase; N-terminally propionylated in vivo
S000005260	YNL316C	PHA2	PHenylAlanine requiring	42071	43075	-1	Prephenate dehydratase	Prephenate dehydratase; catalyzes the conversion of prephanate to phenylpyruvate, which is a step in the phenylalanine biosynthesis pathway
S000005261	YNL317W	PFS2	Polyadenylation Factor Subunit	40619	42016	1	Integral subunit of the pre-mRNA CPF complex	Integral subunit of the pre-mRNA CPF complex; the cleavage and polyadenylation factor (CPF) complex plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex
S000005262	YNL318C	HXT14	HeXose Transporter	38707	40329	-1	Protein with similarity to hexose transporter family members	Protein with similarity to hexose transporter family members; expression is induced in low glucose and repressed in high glucose; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
S000005263	YNL319W	""	""	38642	39082	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HXT14
S000005264	YNL320W	""	""	37700	38554	1	Putative protein of unknown function	Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
S000005265	YNL321W	VNX1	Vacuolar Na+/H+ eXchanger	34696	37422	1	Calcium/H+ antiporter localized to endoplasmic reticulum membrane	Calcium/H+ antiporter localized to endoplasmic reticulum membrane; member of calcium exchanger (CAX) family; targeted to vacuole via AP-3 pathway
S000005266	YNL322C	KRE1	Killer toxin REsistant	33294	34235	-1	Cell wall glycoprotein involved in beta-glucan assembly	Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor
S000005267	YNL323W	LEM3	Ligand-Effect Modulator	31944	33188	1	Membrane protein of the plasma membrane and ER	Membrane protein of the plasma membrane and ER; interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane; null mutant requires tryptophan due to mislocalization of tryptophan permease Tat2p
S000005268	YNL324W	""	""	31050	31445	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000005269	YNL325C	FIG4	Factor-Induced Gene	28739	31378	-1	Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase	Phosphatidylinositol 3,5-bisphosphate (PtdIns[3,5]P) phosphatase; required for efficient mating and response to osmotic shock; physically associates with and regulated by Vac14p; contains a SAC1-like domain; homologous to human FIG4, which is associated with CMT4J, a form of Charcot-Marie-Tooth disorder
S000005270	YNL326C	PFA3	Protein Fatty Acyltransferase	27337	28347	-1	Palmitoyltransferase for Vac8p	Palmitoyltransferase for Vac8p; required for vacuolar membrane fusion; contains an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; autoacylates; required for vacuolar integrity under stress conditions
S000005271	YNL327W	EGT2	Early G1 Transcript	24048	27173	1	Glycosylphosphatidylinositol (GPI)-anchored cell wall endoglucanase	Glycosylphosphatidylinositol (GPI)-anchored cell wall endoglucanase; localizes in birth scars; required for proper cell separation after cytokinesis; expression is activated by Swi5p and tightly regulated in a cell cycle-dependent manner
S000005272	YNL328C	MDJ2	Mitochondrial DnaJ homolog	22834	23274	-1	Constituent of the mitochondrial import motor	Constituent of the mitochondrial import motor; associated with the presequence translocase; function overlaps with that of Pam18p; stimulates the ATPase activity of Ssc1p to drive mitochondrial import; contains a J domain
S000005273	YNL329C	PEX6	PEroXin	19541	22633	-1	AAA-peroxin	AAA-peroxin; heterodimerizes with AAA-peroxin Pex1p and participates in the recycling of peroxisomal signal receptor Pex5p from the peroxisomal membrane to the cystosol; mutations in human PEX6 can lead to severe peroxisomal disorders and early death
S000005274	YNL330C	RPD3	Reduced Potassium Dependency	18001	19302	-1	Histone deacetylase, component of both Rpd3S and Rpd3L complexes	Histone deacetylase, component of both Rpd3S and Rpd3L complexes; regulates transcription, silencing, autophagy and other processes by influencing chromatin remodeling; forms at least two different complexes which have distinct functions and members; Rpd3(L) recruitment to the subtelomeric region is regulated by interaction with the Hmt1p methyltransferase; delays late firing of single copy origins by opposing the Fkh1/2 origin activation pathway
S000005276	YNL332W	THI12	THIamine metabolism	14832	15854	1	Protein involved in synthesis of the thiamine precursor HMP	Protein involved in synthesis of the thiamine precursor HMP; member of a subtelomeric gene family including THI5, THI11, THI12, and THI13; hydroxymethylpyrimidine is also known as HMP
S000005277	YNL333W	SNZ2	SNooZe	13267	14163	1	Protein involved in thiamine and pyridoxine biosynthesis	Protein involved in thiamine and pyridoxine biosynthesis; member of a stationary phase-induced gene family where transcriptional induction precedes the diauxic shift; induced in the absence of thiamin in a Thi2/3p-dependent manner and repressed in its presence; forms a co-regulated gene pair with SNO2; interacts with Thi11p; paralog of SNZ1 and SNZ3
S000005278	YNL334C	SNO2	SNZ proximal Open reading frame	12208	12876	-1	Protein of unknown function	Protein of unknown function; nearly identical to Sno3p; expression is induced before the diauxic shift and also in the absence of thiamin
S000005279	YNL335W	DDI2	DNA Damage Inducible	11452	12129	1	Cyanamide hydratase that detoxifies cyanamide	Cyanamide hydratase that detoxifies cyanamide; member of the HD domain metalloprotein superfamily; expression is induced over 100-fold by cyanamide and by SN2-type DNA alkylating agents such as MMS and DMA; induction decreased in rad6 and rad18 mutants; gene and protein are identical to DDI3 and Ddi3p
S000005280	YNL336W	COS1	COnserved Sequence	8330	9475	1	Endosomal protein involved in turnover of plasma membrane proteins	Endosomal protein involved in turnover of plasma membrane proteins; member of the DUP380 subfamily of conserved, often subtelomeric COS genes; required for the multivesicular vesicle body sorting pathway that internalizes plasma membrane proteins for degradation; Cos proteins provide ubiquitin in trans for nonubiquitinated cargo proteins
S000005282	YNL338W	""	""	6561	6719	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps TEL14L-XC, which is a telomeric X element core sequence on the left arm of chromosome XIV
S000005283	YNL339C	YRF1-6	""	371	6098	-1	Helicase encoded by the Y' element of subtelomeric regions	Helicase encoded by the Y' element of subtelomeric regions; highly expressed in the mutants lacking the telomerase component TLC1; potentially phosphorylated by Cdc28p
S000005284	YNR001C	CIT1	CITrate synthase	629622	631061	-1	Mitochondrial citrate synthase	Mitochondrial citrate synthase; catalyzes condensation of acetyl coenzyme A and oxaloacetate to form citrate, which is the first and rate-limiting step of the TCA cycle; transcription subject to glucose repression; CIT1 has a paralog, CIT2, that arose from the whole genome duplication
S000005285	YNR002C	ATO2	Ammonia (Ammonium) Transport Outward	633008	633856	-1	Putative transmembrane protein involved in export of ammonia	Putative transmembrane protein involved in export of ammonia; ammonia is a starvation signal that promotes cell death in aging colonies; phosphorylated in mitochondria; member of the TC 9.B.33 YaaH family; homolog of Y. lipolytica Gpr1p; ATO2 has a paralog, ADY2, that arose from the whole genome duplication
S000005286	YNR003C	RPC34	RNA Polymerase C	634344	635297	-1	RNA polymerase III subunit C34	RNA polymerase III subunit C34; interacts with TFIIIB70 and is a key determinant in pol III recruitment by the preinitiation complex
S000005287	YNR004W	SWM2	Synthetic With mud2-delta	635943	636383	1	Protein with a role in snRNA and snoRNA cap trimethylation	Protein with a role in snRNA and snoRNA cap trimethylation; interacts with Tgs1p and shows similar phenotypes; required for trimethylation of the caps of spliceosomal snRNAs and the U3 snoRNA, and for efficient 3' end processing of U3 snoRNA; may act as a specificity factor for Tgs1p
S000005288	YNR005C	""	""	636931	637335	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000005289	YNR006W	VPS27	Vacuolar Protein Sorting	636986	638854	1	Ubiquitin binding protein involved in endosomal protein sorting	Ubiquitin binding protein involved in endosomal protein sorting; K48-, K63-Ub chain binding protein that preferentially binds K63-linked chains in vitro; subunit of the ESCRT-0 complex that binds to ubiquitin via two ubiquitin-interacting motifs (UIMs) and is involved in sorting cargo into multivesicular bodies; required for recycling Golgi proteins, forming lumenal membranes and sorting ubiquitinated proteins destined for degradation
S000005290	YNR007C	ATG3	AuTophaGy related	639182	640114	-1	E2-like enzyme	E2-like enzyme; involved in autophagy and cytoplasm-to-vacuole targeting (Cvt) pathway; plays a role in formation of Atg8p-phosphatidylethanolamine conjugates, which are involved in membrane dynamics during autophagy and Cvt; interaction with Atg8p regulated by acetylation by NuA4 histone acetyltransferase Esa1p while attenuation of Atg3 acetylation is mediated by histone deacetylase Rpd3p; Atg12p-Atg5p conjugate enhances E2 activity of Atg3p by rearranging its catalytic site
S000005291	YNR008W	LRO1	Lecithin cholesterol acyl transferase Related Open reading frame	640396	642381	1	Acyltransferase that converts diacylglycerol to triacylglycerol (TGA)	Acyltransferase that converts diacylglycerol to triacylglycerol (TGA); one of several acyltransferases that contribute to TGA synthesis; relocates from ER to nuclear membrane in response cell cycle and starvation signals; Lro1p and Dga1p can O-acylate ceramides; putative homolog of human lecithin cholesterol acyltransferase
S000005292	YNR009W	NRM1	Negative Regulator of MBF targets	642690	643439	1	Transcriptional co-repressor of MBF-regulated gene expression	Transcriptional co-repressor of MBF-regulated gene expression; Nrm1p associates stably with promoters via MCB binding factor (MBF) to repress transcription upon exit from G1 phase
S000005293	YNR010W	CSE2	Chromosome SEgregation	643744	644193	1	Subunit of the RNA polymerase II mediator complex	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; component of the Middle domain of mediator; required for regulation of RNA polymerase II activity; relocalizes to the cytosol in response to hypoxia
S000005294	YNR011C	PRP2	Pre-mRNA Processing	644320	646950	-1	RNA-dependent DExD/H-box ATPase	RNA-dependent DExD/H-box ATPase; required for activation of spliceosome before first transesterification step in RNA splicing; implicated in rearranging and proofreading snRNA structure in catalytic activation of spliceosome; ortholog of human protein DHX16
S000005295	YNR012W	URK1	URidine Kinase	647432	648937	1	Uridine/cytidine kinase	Uridine/cytidine kinase; component of the pyrimidine ribonucleotide salvage pathway that converts uridine into UMP and cytidine into CMP; involved in the pyrimidine deoxyribonucleotide salvage pathway, converting deoxycytidine into dCMP
S000005296	YNR013C	PHO91	PHOsphate metabolism	649028	651712	-1	Low-affinity vacuolar phosphate transporter	Low-affinity vacuolar phosphate transporter; exports phosphate from vacuolar lumen to cytosol; regulates phosphate and polyphosphate metabolism; acts upstream of Pho81p in regulation of PHO pathway; localizes to sites of contact between vacuole and mitochondria (vCLAMPs); targeted to vacuole via AP-3 pathway; transcription independent of Pi and Pho4p activity; overexpression results in vigorous growth
S000005297	YNR014W	""	""	652465	653103	1	Putative protein of unknown function	Putative protein of unknown function; expression is cell-cycle regulated, Azf1p-dependent, and heat-inducible; YNR014W has a paralog, YMR206W, that arose from the whole genome duplication
S000005298	YNR015W	SMM1	Suppressor of Mitochondrial Mutation	653387	654541	1	Dihydrouridine synthase	Dihydrouridine synthase; member of a family of dihydrouridine synthases including Dus1p, Smm1p, Dus3p, and Dus4p; modifies uridine residues at position 20 of cytoplasmic tRNAs
S000005299	YNR016C	ACC1	Acetyl-CoA Carboxylase	654673	661374	-1	Acetyl-CoA carboxylase, biotin containing enzyme	Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availability of acetyl-CoA; rate-limiting step for de novo biosynthesis of long-chain fatty acids; translational regulation in response to nutrients and cell cycle stage depends on its upstream ORF; ACC1 has a paralog, HFA1, that arose from the whole genome duplication
S000005300	YNR017W	TIM23	Translocase of the Inner Mitochondrial membrane	662913	663581	1	Essential core component of the TIM23 complex	Essential core component of the TIM23 complex; subunit of the translocase of the inner mitochondrial membrane involved in protein import into the mitochondrial matrix and inner membrane; provides a structural role, contributing to the architecture of the import channel; functions as a voltage sensor and pre-sequence receptor
S000005301	YNR018W	RCF2	Respiratory superComplex Factor	664270	664944	1	Cytochrome c oxidase subunit	Cytochrome c oxidase subunit; has a role in assembly of respiratory supercomplexes; similar to Rcf1p, and either Rcf1p or Rcf2p is required for late-stage assembly of the Cox12p and Cox13p subunits and for cytochrome c oxidase activity; associates with the cytochrome c oxidase - cytochrome bc1 supercomplex; null mutant accumulates reactive oxygen species; member of the conserved hypoxia induced gene family; C. elegans homolog is functional in yeast
S000005302	YNR019W	ARE2	Acyl-coenzyme A: cholesterol acyl transferase-Related Enzyme	665339	667267	1	Acyl-CoA:sterol acyltransferase	Acyl-CoA:sterol acyltransferase; endoplasmic reticulum enzyme that contributes the major sterol esterification activity in the presence of oxygen; ARE2 has a paralog, ARE1, that arose from the whole genome duplication
S000005303	YNR020C	ATP23	""	667410	668222	-1	Putative metalloprotease of the mitochondrial inner membrane	Putative metalloprotease of the mitochondrial inner membrane; required for processing of Atp6p; has an additional role in assembly of the F0 sector of the F1F0 ATP synthase complex; substrate of the Mia40p-Erv1p disulfide relay system, and folding is assisted by Mia40p
S000005304	YNR021W	""	""	668377	669591	1	Putative protein of unknown function	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; YNR021W is not an essential gene
S000005305	YNR022C	MRPL50	Mitochondrial Ribosomal Protein, Large subunit	669774	670193	-1	Mitochondrial ribosomal protein of the large subunit	Mitochondrial ribosomal protein of the large subunit; not essential for mitochondrial translation
S000005307	YNR024W	MPP6	M-Phase Phosphoprotein 6 homolog	672409	672969	1	Nuclear exosome-associated RNA binding protein	Nuclear exosome-associated RNA binding protein; involved in surveillance of pre-rRNAs and pre-mRNAs, and the degradation of cryptic non-coding RNAs (ncRNA); copurifies with ribosomes; relocalizes to the cytosol in response to hypoxia
S000005308	YNR025C	""	""	672702	673061	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; deletion reduces expression of PIS1 gene encoding phosphatidylinositol synthase
S000005309	YNR026C	SEC12	SECretory	673274	674689	-1	Guanine nucleotide exchange factor (GEF)	Guanine nucleotide exchange factor (GEF); activates Sar1p by catalyzing the exchange of GDP for GTP; required for the initiation of COPII vesicle formation in ER to Golgi transport; glycosylated integral membrane protein of the ER; SEC12 has a paralog, SED4, that arose from the whole genome duplication
S000005310	YNR027W	BUD17	BUD site selection	674923	675876	1	Putative pyridoxal kinase	Putative pyridoxal kinase; a key enzyme in vitamin B6 metabolism; involved in bud-site selection; diploid mutants display a random rather than a bipolar budding pattern; similarity to yeast BUD16 and human pyridoxal kinase (PDXK)
S000005311	YNR028W	CPR8	Cyclosporin-sensitive Proline Rotamase	676177	677103	1	Peptidyl-prolyl cis-trans isomerase (cyclophilin)	Peptidyl-prolyl cis-trans isomerase (cyclophilin); catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; potential role in the secretory pathway; CPR8 has a paralog, CPR4, that arose from the whole genome duplication
S000005312	YNR029C	ZNG1	Zn-regulated GTPase metalloprotein activator	677199	678488	-1	GTP-dependent zinc transferase	GTP-dependent zinc transferase; couples GTP hydrolysis to the activation of methionine aminopeptidase Map1p, especially during zinc starvation; deletion confers reduced fitness in saline; contains GTPase sequence motifs and a CxCC metal-binding motif
S000005313	YNR030W	ALG12	Asparagine-Linked Glycosylation	678799	680454	1	Alpha-1,6-mannosyltransferase localized to the ER	Alpha-1,6-mannosyltransferase localized to the ER; responsible for addition of alpha-1,6 mannose to dolichol-linked Man7GlcNAc2; acts in the dolichol pathway for N-glycosylation; human homolog ALG12 complements yeast null mutant
S000005314	YNR031C	SSK2	Suppressor of Sensor Kinase	680694	685433	-1	MAP kinase kinase kinase of HOG1 mitogen-activated signaling pathway	MAP kinase kinase kinase of HOG1 mitogen-activated signaling pathway; interacts with Ssk1p, leading to autophosphorylation and activation of Ssk2p which phosphorylates Pbs2p; also mediates actin cytoskeleton recovery from osmotic stress; a HOG-independent function of Ssk2p mediates the calcium-sensitive phenotype of the ptp2 msg5 double disruptant; SSK2 has a paralog, SSK22, that arose from the whole genome duplication
S000005315	YNR032W	PPG1	Protein Phosphatase involved in Glycogen accumulation	686010	687116	1	Serine protein phosphatase involved in formation of FAR complex	Serine protein phosphatase involved in formation of FAR complex; inhibits mitophagy by dephosphorylating Atg32p; required for glycogen accumulation; interacts with Tap42p, which binds to and regulates other protein phosphatases
S000005316	YNR033W	ABZ1	para-AminoBenZoic acid (PABA) biosynthesis	687635	689998	1	Para-aminobenzoate (PABA) synthase	Para-aminobenzoate (PABA) synthase; has similarity to Escherichia coli PABA synthase components PabA and PabB; required for the synthesis of para-aminobenzoic acid, an important intermediate for folate and ubiquinone Q biosynthesis; protein abundance increases in response to DNA replication stress
S000005317	YNR034W	SOL1	Suppressor Of Los1-1	690321	691286	1	Protein with a possible role in tRNA export	Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL1 has a paralog, SOL2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
S000005318	YNR035C	ARC35	ARp2/3 Complex subunit	693019	694047	-1	Subunit of the ARP2/3 complex	Subunit of the ARP2/3 complex; ARP2/3 is required for the motility and integrity of cortical actin patches; required for cortical localization of calmodulin
S000005319	YNR036C	MRPS12	Mitochondrial Ribosomal Protein, Small subunit	694361	694822	-1	Mitochondrial protein	Mitochondrial protein; may interact with ribosomes based on co-purification experiments; similar to E. coli and human mitochondrial S12 ribosomal proteins
S000005320	YNR037C	RSM19	Ribosomal Small subunit of Mitochondria	695052	695327	-1	Mitochondrial ribosomal protein of the small subunit	Mitochondrial ribosomal protein of the small subunit; has similarity to E. coli S19 ribosomal protein
S000005321	YNR038W	DBP6	Dead Box Protein	695595	697484	1	Essential protein involved in ribosome biogenesis	Essential protein involved in ribosome biogenesis; putative ATP-dependent RNA helicase of the DEAD-box protein family; human homolog DDX51 complements yeast dbp6 mutant
S000005322	YNR039C	ZRG17	Zinc Regulated Gene	697614	699431	-1	Endoplasmic reticulum zinc transporter	Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Msc2p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; zinc-regulated directly through Zap1p; transcription induced under conditions of zinc deficiency
S000005323	YNR040W	MRX15	Mitochondrial oRganization of gene eXpression (MIOREX)	699690	700460	1	Membrane-associated mitoribosome receptor	Membrane-associated mitoribosome receptor; involved along with Mba1p in the cotranslational insertion of nascent polypeptide chains into the inner mitochondrial membrane; associates with the mitochondrial large ribosomal subunit and nascent polypeptide chains; null mutant displays increased tolerance to mistranslation-induced proteotoxic stress; localizes to the inner mitochondrial membrane
S000005324	YNR041C	COQ2	COenzyme Q	700541	701659	-1	Para hydroxybenzoate polyprenyl transferase	Para hydroxybenzoate polyprenyl transferase; catalyzes the second step in ubiquinone (coenzyme Q) biosynthesis; human COQ2, mutations in which are implicated in an increased risk of mutiple-system atrophy, can complement a yeast coq2 null mutant
S000005325	YNR042W	""	""	701238	701666	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps verified gene COQ2
S000005326	YNR043W	MVD1	MeValonate pyrophosphate Decarboxylase	701895	703085	1	Mevalonate pyrophosphate decarboxylase	Mevalonate pyrophosphate decarboxylase; essential enzyme involved in the biosynthesis of isoprenoids and sterols, including ergosterol; acts as a homodimer
S000005328	YNR045W	PET494	PETite colonies	706139	707608	1	Mitochondrial translational activator specific for the COX3 mRNA	Mitochondrial translational activator specific for the COX3 mRNA; acts together with Pet54p and Pet122p; located in the mitochondrial inner membrane
S000005329	YNR046W	TRM112	TRna Methyltransferase	707788	708195	1	Protein involved in methylation of tRNA, rRNA, and translation factors	Protein involved in methylation of tRNA, rRNA, and translation factors; also involved in ribosome biogenesis; subunit of tRNA methyltransferase (MTase) complexes in combination with Trm9p and Trm11p; N7-methylates G1575 of 18S rRNA as complex with Bud23p; subunit of complex with Mtq2p that methylates Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; relative distribution to the nucleus increases upon DNA replication stress; functional homolog of human TRMT112
S000005330	YNR047W	FPK1	FliPase Kinase	708523	711204	1	Ser/Thr protein kinase	Ser/Thr protein kinase; phosphorylates several aminophospholipid translocase family members, regulating phospholipid translocation and membrane asymmetry; phosphorylates and inhibits the protein kinase Akl1p, stimulating endocytosis; phosphorylates and inhibits upstream inhibitory kinase Ypk1p; localizes to the cytoplasm, early endosome/TGN, the plasma membrane and the shmoo tip; redundant role with KIN82 in the mating pheromone response; activity stimulated by Gin4p kinase and sphingolipid MIPC
S000005331	YNR048W	""	""	711630	712811	1	Potential noncatalytic subunit for phospholipid translocase Dnf3p	Potential noncatalytic subunit for phospholipid translocase Dnf3p; YNR048W has a paralog, CDC50, that arose from the whole genome duplication
S000005332	YNR049C	MSO1	Multicopy suppressor of Sec One	713023	713655	-1	Lipid-interacting protein in SNARE complex assembly machinery	Lipid-interacting protein in SNARE complex assembly machinery; acts at late step in secretion; interacts with membranes through two distinct binding sites; shows genetic and physical interactions with Sec1p; required for prospore membrane formation during sporulation; N-terminus closely associates with plasma membrane, C-terminus colocalizes with Sec4p on intracellular membranes; relocalizes from bud neck to nucleus upon DNA replication stress
S000005333	YNR050C	LYS9	LYSine requiring	714048	715388	-1	Saccharopine dehydrogenase (NADP+, L-glutamate-forming)	Saccharopine dehydrogenase (NADP+, L-glutamate-forming); catalyzes the formation of saccharopine from alpha-aminoadipate 6-semialdehyde, the seventh step in lysine biosynthesis pathway; exhibits genetic and physical interactions with TRM112
S000005334	YNR051C	BRE5	BREfeldin A sensitivity	716780	718327	-1	Ubiquitin protease cofactor	Ubiquitin protease cofactor; forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the ER and Golgi compartments, deubiquitinating COPII and COPI vesicle coat constituents, Sec23p and Sec27p; involved along with Ubp3p in the steady-state retention of Golgi membrane proteins, such as glycosyltransferases; null is sensitive to brefeldin A
S000005335	YNR052C	POP2	PGK promoter directed OverProduction	719346	720647	-1	Subunit of Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation	Subunit of Ccr4-Not complex that mediates 3' to 5' mRNA deadenylation; exonuclease of the DEDD superfamily
S000005336	YNR053C	NOG2	NucleOlar G-protein	721120	723112	-1	Putative GTPase	Putative GTPase; associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation; recruited by ribosomal proteins L17, L35, and L37 to assembling ribosomes after 27SB pre-rRNA is generated, immediately preceding removal of ITS2
S000005337	YNR054C	ESF2	Eighteen S rRNA Factor	723356	724306	-1	Essential nucleolar protein involved in pre-18S rRNA processing	Essential nucleolar protein involved in pre-18S rRNA processing; binds to RNA and stimulates ATPase activity of Dbp8; involved in assembly of the small subunit (SSU) processome
S000005338	YNR055C	HOL1	HistidinOl	728426	730186	-1	Polyamine transporter of the major facilitator superfamily	Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; mutations in membrane-spanning domains permit cation and histidinol uptake
S000005339	YNR056C	BIO5	BIOtin	731618	733303	-1	Putative transmembrane protein involved in the biotin biosynthesis	Putative transmembrane protein involved in the biotin biosynthesis; responsible for uptake of 7-keto 8-aminopelargonic acid; BIO5 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis
S000005340	YNR057C	BIO4	BIOtin	733356	734069	-1	Dethiobiotin synthetase	Dethiobiotin synthetase; catalyzes the third step in the biotin biosynthesis pathway; BIO4 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; BIO3 and BIO4 were acquired by horizontal gene transfer (HGT) from bacteria; expression appears to be repressed at low iron levels
S000005341	YNR058W	BIO3	BIOtin	734291	735733	1	7,8-diamino-pelargonic acid aminotransferase (DAPA)	7,8-diamino-pelargonic acid aminotransferase (DAPA); catalyzes the second step in the biotin biosynthesis pathway; BIO3 is in a cluster of 3 genes (BIO3, BIO4, and BIO5) that mediate biotin synthesis; BIO3 and BIO4 were acquired by horizontal gene transfer (HGT) from bacteria
S000005342	YNR059W	MNT4	MaNnosylTransferase	736803	738545	1	Putative alpha-1,3-mannosyltransferase	Putative alpha-1,3-mannosyltransferase; not required for protein O-glycosylation; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum and vacuole respectively
S000005343	YNR060W	FRE4	Ferric REductase	739951	742110	1	Ferric reductase	Ferric reductase; reduces a specific subset of siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels
S000005344	YNR061C	""	""	742881	743540	-1	Protein of unknown function	Protein of unknown function; relocalizes from vacuole to cytoplasm upon DNA replication stress
S000005345	YNR062C	PUL3	PULcherrimin	744360	745343	-1	Putative pulcherrimin transporter	Putative pulcherrimin transporter; involved in uptake of iron-transporting siderophore, pulcherrimin
S000005346	YNR063W	PUL4	PULcherrimin	746943	748766	1	Putative zinc-cluster transcription factor	Putative zinc-cluster transcription factor; involved in utilization of iron-transporting siderophore pulcherrimin
S000005347	YNR064C	""	""	749136	750008	-1	Epoxide hydrolase	Epoxide hydrolase; member of the alpha/beta hydrolase fold family; may have a role in detoxification of epoxides
S000005348	YNR065C	""	""	750350	753700	-1	Protein of unknown function	Protein of unknown function; protein-protein interactions suggest a possible role in actin patch formation; YNR065C is not an essential gene
S000005349	YNR066C	""	""	753725	755035	-1	Putative membrane-localized protein of unknown function	Putative membrane-localized protein of unknown function
S000005350	YNR067C	DSE4	Daughter Specific Expression	755746	759099	-1	Daughter cell-specific secreted protein with similarity to glucanases	Daughter cell-specific secreted protein with similarity to glucanases; degrades cell wall from the daughter side causing daughter to separate from mother
S000005351	YNR068C	""	""	760067	760885	-1	Putative protein of unknown function	Putative protein of unknown function; exhibits homology to C-terminal end of Bul1p; expressed as a readthrough product of BSC5, the readthrough locus being termed BUL3; the BUL3 readthrough product is involved in ubiquitin-mediated sorting of plasma membrane proteins and interacts with WW domains of Rsp5p in vitro, but in a functionally different way than the non-readthrough form
S000005352	YNR069C	BSC5	Bypass of Stop Codon	761123	762592	-1	Protein of unknown function	Protein of unknown function; shows homology with N-terminal end of Bul1p; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression; readthrough expression includes YNR068C and the locus for this readthrough is termed BUL3; Bul3p is involved in ubiquitin-mediated sorting of plasma membrane proteins; readthrough and shortened forms of Bul3p interact with Rsp5p differently in vitro
S000005353	YNR070W	PDR18	Pleiotropic Drug Resistance	765375	769376	1	Transporter of the ATP-binding cassette (ABC) family	Transporter of the ATP-binding cassette (ABC) family; role in plasma membrane sterol incorporation; implicated in pleiotropic drug resistance; provides resistance to ethanol stress and contributes to a decreased intracellular accumulation of ethanol; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
S000005354	YNR071C	""	""	770440	771468	-1	Putative aldose 1-epimerase	Putative aldose 1-epimerase
S000005355	YNR072W	HXT17	HeXose Transporter	772657	774351	1	Putative transmembrane polyol transporter	Putative transmembrane polyol transporter; supports growth on and uptake of mannitol and sorbitol with moderate affinity when overexpressed in a strain deleted for hexose family members; minor hexose transport activity when overexpressed in a similar strain; induced by raffinose and galactose at pH 7.7 versus pH 4.7, repressed by high levels of glucose; HXT17 has a paralog, HXT13, that arose from a segmental duplication
S000005356	YNR073C	MAN2	MANnitol dehydrogenase	774792	776300	-1	Mannitol dehydrogenase	Mannitol dehydrogenase; MAN2 has a paralog, DSF1, that arose from a segmental duplication
S000005357	YNR074C	AIF1	Apoptosis-Inducing Factor	777602	778738	-1	Mitochondrial cell death effector	Mitochondrial cell death effector; translocates to the nucleus in response to apoptotic stimuli; triggers apoptosis in cells lacking HXK2; homolog of mammalian Apoptosis-Inducing Factor, putative reductase
S000005358	YNR075W	COS10	COnserved Sequence	779916	781040	1	Endosomal protein involved in turnover of plasma membrane proteins	Endosomal protein involved in turnover of plasma membrane proteins; member of the DUP380 subfamily of conserved, often subtelomeric COS genes; required for the multivesicular vesicle body sorting pathway that internalizes plasma membrane proteins for degradation; Cos proteins provide ubiquitin in trans for nonubiquitinated cargo proteins
S000005361	YOL001W	PHO80	PHOsphate metabolism	325249	326130	1	Cyclin	Cyclin; interacts with cyclin-dependent kinase Pho85p; regulates the response to nutrient levels and environmental conditions, including the response to phosphate limitation and stress-dependent calcium signaling
S000005362	YOL002C	IZH2	Implicated in Zinc Homeostasis	323411	324364	-1	Plasma membrane receptor for plant antifungal osmotin	Plasma membrane receptor for plant antifungal osmotin; involved in zinc ion homeostasis, apoptosis; negatively regulates ZRT1 and other functionally divergent genes through CCCTC promoter motif (IzRE); modulates FET3 activity in iron-independent manner; affects gene expression by influencing balance of competition between Msn2p/Msn4p and Nrg1p/Nrg2p for binding to IzRE; transcription regulated by Zap1p, zinc, fatty acid levels; homolog of mammalian adiponectin receptor
S000005363	YOL003C	PFA4	Protein Fatty Acyltransferase	321858	322994	-1	Palmitoyltransferase with autoacylation activity	Palmitoyltransferase with autoacylation activity; required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
S000005364	YOL004W	SIN3	Switch INdependent	316938	321548	1	Component of both the Rpd3S and Rpd3L histone deacetylase complexes	Component of both the Rpd3S and Rpd3L histone deacetylase complexes; involved in transcriptional repression and activation of diverse processes, including mating-type switching and meiosis; involved in the maintenance of chromosomal integrity
S000005365	YOL005C	RPB11	RNA Polymerase B	315813	316175	-1	RNA polymerase II subunit B12.5	RNA polymerase II subunit B12.5; part of central core; similar to Rpc19p and bacterial alpha subunit
S000005366	YOL006C	TOP1	TOPoisomerase	313078	315387	-1	Topoisomerase I	Topoisomerase I; nuclear enzyme that relieves torsional strain in DNA by cleaving and re-sealing the phosphodiester backbone; relaxes both positively and negatively supercoiled DNA; functions in replication, transcription, and recombination; role in processing ribonucleoside monophosphates in genomic DNA into irreversible single-strand breaks; enzymatic activity and interaction with Nsr1p are negatively regulated by polyphosphorylation
S000005367	YOL007C	CSI2	Chitin Synthesis Involved	311342	312367	-1	Protein of unknown function	Protein of unknown function; green fluorescent protein (GFP)- fusion protein localizes to the mother side of the bud neck and the vacuole; YOL007C is not an essential gene
S000005368	YOL008W	COQ10	COenzyme Q	310312	310935	1	Coenzyme Q (ubiquinone) binding protein	Coenzyme Q (ubiquinone) binding protein; functions in the delivery of Q<sub>6</sub> to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes; respiratory growth defect of the null mutant is functionally complemented by human COQ10A
S000005369	YOL009C	MDM12	Mitochondrial Distribution and Morphology	309324	310139	-1	Mitochondrial outer membrane protein, ERMES complex subunit	Mitochondrial outer membrane protein, ERMES complex subunit; required for transmission of mitochondria to daughter cells; required for mitophagy; may influence import and assembly of outer membrane beta-barrel proteins; localizes to mitochondria–ER contact sites; ERMES complex is often co-localized with peroxisomes and with concentrated areas of pyruvate dehydrogenase
S000005370	YOL010W	RCL1	Rna 3'-terminal phosphate Cyclase Like	307938	309041	1	Endonuclease that cleaves pre-rRNA at site A2 for 18S rRNA biogenesis	Endonuclease that cleaves pre-rRNA at site A2 for 18S rRNA biogenesis; subunit of U3-containing 90S preribosome processome complex involved in small ribosomal subunit assembly; stimulates Bms1p GTPase and U3 binding activity; similar to RNA cyclase-like proteins but no cyclase activity detected
S000005371	YOL011W	PLB3	PhosphoLipase B	305349	307409	1	Phospholipase B (lysophospholipase) involved in lipid metabolism	Phospholipase B (lysophospholipase) involved in lipid metabolism; hydrolyzes phosphatidylinositol and phosphatidylserine and displays transacylase activity in vitro; PLB3 has a paralog, PLB1, that arose from the whole genome duplication
S000005372	YOL012C	HTZ1	Histone Two A Z	303579	303983	-1	Histone variant H2A.Z	Histone variant H2A.Z; exchanged for histone H2A in nucleosomes by the SWR1 complex; involved in transcriptional regulation through prevention of the spread of silent heterochromatin; Htz1p-containing nucleosomes facilitate RNA Pol II passage by affecting correct assembly and modification status of RNA Pol II elongation complexes and by favoring efficient nucleosome remodeling
S000005373	YOL013C	HRD1	HMG-coA Reductase Degradation	301380	303035	-1	Ubiquitin-protein ligase involved in ER-associated degradation (ERAD)	Ubiquitin-protein ligase involved in ER-associated degradation (ERAD) of misfolded proteins; upon autoubiquitination triggers retrotranslocation of misfolded proteins to cytosol for degradation; genetically linked to the unfolded protein response (UPR); regulated through association with Hrd3p; contains an H2 ring finger; likely plays a general role in targeting proteins that persistently associate with and potentially obstruct the ER-localized translocon
S000005374	YOL014W	""	""	299693	300067	1	Putative protein of unknown function	Putative protein of unknown function; mCherry fusion protein localizes to the cytosol and nucleus
S000005375	YOL015W	IRC10	Increased Recombination Centers	297078	298838	1	Protein of unknown function	Protein of unknown function; subunit of the leading edge protein (LEP) complex (Ssp1-Ady3-Don1-Irc10) that forms a ring-like structure at the leading edge of the prospore membrane during meiosis II; null mutant displays increased levels of spontaneous Rad52p foci
S000005376	YOL016C	CMK2	CalModulin dependent protein Kinase	294777	296120	-1	Calmodulin-dependent protein kinase	Calmodulin-dependent protein kinase; negative feedback controller of calcium/calcineurin signalling pathway, also has additional Crz1-independent role in promoting calcium tolerance; amino acid sequence similar to mammalian Cam Kinase II; CMK2 has a paralog, CMK1, that arose from the whole genome duplication
S000005377	YOL017W	ESC8	Establishes Silent Chromatin	292529	294673	1	Protein involved in telomeric and mating-type locus silencing	Protein involved in telomeric and mating-type locus silencing; interacts with Sir2p and also interacts with Gal11p, which is a component of the RNA pol II mediator complex; ESC8 has a paralog, IOC3, that arose from the whole genome duplication
S000005378	YOL018C	TLG2	T-snare affecting a Late Golgi compartment	290881	292074	-1	Syntaxin-like t-SNARE	Syntaxin-like t-SNARE; forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; required along with VPS45 for an early step of the constitutive CVT pathway; interactions with Vps45 prevents Tlg2p degradation, and facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16)
S000005379	YOL019W	TOS7	Target Of Sbf	288898	290553	1	Protein involved in secretion and cell wall organization	Protein involved in secretion and cell wall organization; localizes to plasma membrane microdomains called MCCs (membrane compartment occupied by Can1); GFP-fusion protein localizes to the cell periphery and vacuole; Sur7/PalI family transmembrane domain (TMD) containing protein; TOS7 has a paralog, DCV1, that arose from the whole genome duplication
S000005380	YOL020W	TAT2	Tryptophan Amino acid Transporter	286172	287950	1	High affinity tryptophan and tyrosine permease	High affinity tryptophan and tyrosine permease; overexpression confers FK506 and FTY720 resistance
S000005381	YOL021C	DIS3	homolog of S. pombe dis3 (chromosome DISjunction)	282421	285426	-1	Exosome core complex catalytic subunit	Exosome core complex catalytic subunit; has both endonuclease and 3'-5' exonuclease activity; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; role in degradation of tRNAs; similar to E. coli RNase R and to human DIS3, which partially complements dis3-81 heat sensitivity; mutations in Dis3p analogous to human mutations implicated in multiple myeloma impair exosome function; protein abundance increases under to DNA replication stress
S000005382	YOL022C	TSR4	Twenty S rRNA accumulation	280272	281498	-1	Cytoplasmic assembly chaperone for ribosomal protein Rps2p	Cytoplasmic assembly chaperone for ribosomal protein Rps2p; co-translationally associates with the N-terminal extension of Rps2p to facilitate expression, solubility and ribosome assembly; released prior to the nuclear import of Rps2p; required for correct processing of 20S pre-rRNA at site D to generate mature 18S rRNA; essential gene in the S288C background but not in CEN.PK2; homologous to human PDCD2L and PDCD2
S000005383	YOL023W	IFM1	Initiation Factor of Mitochondria	278056	280086	1	Mitochondrial translation initiation factor 2	Mitochondrial translation initiation factor 2
S000005384	YOL024W	""	""	277086	277604	1	Putative protein of unknown function	Putative protein of unknown function; predicted to have thiol-disulfide oxidoreductase active site; YOL024W has a paralog, IGD1, that arose from the whole genome duplication
S000005385	YOL025W	LAG2	Longevity Assurance Gene	274957	276939	1	Protein that negatively regulates the SCF E3-ubiquitin ligase	Protein that negatively regulates the SCF E3-ubiquitin ligase; regulates by interacting with and preventing neddyation of the cullin subunit, Cdc53p; longevity determinant that is preferentially expressed in young cells; similar to mammalian Cand1
S000005386	YOL026C	MIM1	Mitochondrial IMport	274012	274353	-1	Mitochondrial protein required for outer membrane protein import	Mitochondrial protein required for outer membrane protein import; cooperates with Tom70p to import the subset of proteins with multiple alpha-helical transmembrane segments, including Ugo1p, Tom20p, and others; forms a complex with Mim2p in the outer membrane that functions as cation-selective channel for translocation of positively charged precursor segments; also has a role in assembly of Tom20p into the TOM complex
S000005387	YOL027C	MDM38	Mitochondrial Distribution and Morphology	272003	273724	-1	Membrane-associated mitochondrial ribosome receptor	Membrane-associated mitochondrial ribosome receptor; forms a complex with Mba1p to facilitate recruitment of mRNA-specific translational activators to ribosomes; ribosome-associated protein involved in the insertion of newly synthesized proteins into the mitochondrial inner membrane; role in protein export and K+/H+ exchange; localizes to the inner mitochondrial membrane; human ortholog Letm1 implicated in Wolf-Hirschhorn syndrome
S000005388	YOL028C	YAP7	Yeast AP-1	270633	271370	-1	Transcriptional repressor of nitric oxide oxidase	Transcriptional repressor of nitric oxide oxidase; basic leucine zipper (bZIP) transcription factor; neighboring gene, YOL029C, created by frame-shift at position 708, corresponds to C-terminal part of YAP7 orthologs in other species; YAP7 has a paralog, YAP5, that arose from the whole genome duplication
S000005389	YOL029C	""	""	269815	270420	-1	Putative protein of unknown function	Putative protein of unknown function; separated from neighboring gene, YAP7, by frame shift and corresponds to C-terminal part of YAP7 orthologs in other species; identified as interacting with Hsc82p and Hsp82p in high-throughput two-hybrid screens
S000005390	YOL030W	GAS5	Glycophospholipid-Anchored Surface protein	268187	269641	1	1,3-beta-glucanosyltransferase	1,3-beta-glucanosyltransferase; has similarity to Gas1p; localizes to the cell wall
S000005391	YOL031C	SIL1	Suppressor of the Ire1/Lhs1 double mutant	266264	267529	-1	Nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p	Nucleotide exchange factor for the ER lumenal Hsp70 chaperone Kar2p; required for protein translocation into the endoplasmic reticulum (ER); homolog of Yarrowia lipolytica SLS1; GrpE-like protein
S000005392	YOL032W	OPI10	OverProducer of Inositol	265429	266169	1	Protein with a possible role in phospholipid biosynthesis	Protein with a possible role in phospholipid biosynthesis; null mutant displays an inositol-excreting phenotype that is suppressed by exogenous choline; protein abundance increases in response to DNA replication stress
S000005393	YOL033W	MSE1	Mitochondrial aminoacyl-tRNA Synthetase, Glutamate (E)	263475	265085	1	Mitochondrial glutamyl-tRNA synthetase	Mitochondrial glutamyl-tRNA synthetase; predicted to be palmitoylated
S000005394	YOL034W	SMC5	Structural Maintenance of Chromosomes	259923	263204	1	Subunit of the SMC5-SMC6 complex	Subunit of the SMC5-SMC6 complex; the SMC5-SMC6 complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; binds single-stranded DNA and has ATPase activity; supports nucleolar function; S. pombe homolog forms a heterodimer with S. pombe Rad18p that is involved in DNA repair
S000005395	YOL035C	""	""	258803	259105	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000005396	YOL036W	""	""	256745	259030	1	Protein of unknown function	Protein of unknown function; potential Cdc28p substrate; YOL036W has a paralog, YIR016W, that arose from the whole genome duplication
S000005397	YOL037C	""	""	256666	257019	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOL036W
S000005398	YOL038W	PRE6	PRoteinase yscE	255337	256101	1	Alpha 4 subunit of the 20S proteasome	Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress
S000005399	YOL039W	RPP2A	Ribosomal Protein P2 Alpha	254297	254617	1	Ribosomal protein P2 alpha	Ribosomal protein P2 alpha; a component of the ribosomal stalk, which is involved in the interaction between translational elongation factors and the ribosome; free (non-ribosomal) P2 stimulates the phosphorylation of the eIF2 alpha subunit (Sui2p) by Gcn2p; regulates the accumulation of P1 (Rpp1Ap and Rpp1Bp) in the cytoplasm
S000005401	YOL041C	NOP12	NucleOlar Protein	251267	252646	-1	Nucleolar protein involved in pre-25S rRNA processing	Nucleolar protein involved in pre-25S rRNA processing; also involved in biogenesis of large 60S ribosomal subunit; contains an RNA recognition motif (RRM); binds to Ebp2; similar to Nop13p and Nsr1p
S000005402	YOL042W	NGL1	""	249825	250916	1	Putative endonuclease	Putative endonuclease; has a domain similar to a magnesium-dependent endonuclease motif in mRNA deadenylase Ccr4p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
S000005403	YOL043C	NTG2	eNdonuclease Three-like Glycosylase	248392	249534	-1	DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase	DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair, localizes to the nucleus; sumoylated; NTG2 has a paralog, NTG1, that arose from the whole genome duplication
S000005404	YOL044W	PEX15	PEroXisome related	247150	248301	1	Tail-anchored type II integral peroxisomal membrane protein	Tail-anchored type II integral peroxisomal membrane protein; required for peroxisome biogenesis; cells lacking Pex15p mislocalize peroxisomal matrix proteins to cytosol; overexpression results in impaired peroxisome assembly
S000005405	YOL045W	PSK2	Pas domain-containing Serine/threonine protein Kinase	243497	246802	1	PAS-domain containing serine/threonine protein kinase	PAS-domain containing serine/threonine protein kinase; regulates sugar flux and translation in response to an unknown metabolite by phosphorylating Ugp1p and Gsy2p (sugar flux) and Caf20p, Tif11p and Sro9p (translation); PSK2 has a paralog, PSK1, that arose from the whole genome duplication
S000005406	YOL046C	""	""	243466	244140	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene PSK2/YOL045W
S000005407	YOL047C	LDS2	Lipid Droplets in Sporulation	241614	242747	-1	Protein Involved in spore wall assembly	Protein Involved in spore wall assembly; localizes to lipid droplets found on or outside of the prospore membrane; shares similarity with Lds1p and Rrt8p, and a strain mutant for all 3 genes exhibits reduced dityrosine fluorescence relative to the single mutants; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
S000005408	YOL048C	RRT8	Regulator of rDNA Transcription	240204	241310	-1	Protein involved in spore wall assembly	Protein involved in spore wall assembly; shares similarity with Lds1p and Lds2p and a strain mutant for all 3 genes exhibits reduced dityrosine fluorescence relative to the single mutants; identified in a screen for mutants with increased levels of rDNA transcription; green fluorescent protein (GFP)-fusion protein localizes to lipid particles; protein abundance increases in response to DNA replication stress
S000005409	YOL049W	GSH2	Glutathione	238619	240094	1	Glutathione synthetase	Glutathione synthetase; catalyzes the ATP-dependent synthesis of glutathione (GSH) from gamma-glutamylcysteine and glycine; induced by oxidative stress and heat shock
S000005410	YOL050C	""	""	237882	238202	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)
S000005411	YOL051W	GAL11	GALactose metabolism	234940	238185	1	Subunit of the RNA polymerase II mediator complex	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; affects transcription by acting as target of activators and repressors; forms part of the tail domain of mediator
S000005412	YOL052C	SPE2	SPErmidine auxotroph	232446	233636	-1	S-adenosylmethionine decarboxylase	S-adenosylmethionine decarboxylase; required for the biosynthesis of spermidine and spermine; cells lacking Spe2p require spermine or spermidine for growth in the presence of oxygen but not when grown anaerobically
S000005413	YOL052C-A	DDR2	DNA Damage Responsive	231570	231755	-1	Multi-stress response protein	Multi-stress response protein; expression is activated by a variety of xenobiotic agents and environmental or physiological stresses; DDR2 has a paralog, HOR7, that arose from the whole genome duplication
S000005414	YOL053W	AIM39	Altered Inheritance rate of Mitochondria	230085	231272	1	Protein of unknown function	Protein of unknown function; null mutant displays elevated frequency of mitochondrial genome loss; localizes to mitochondria, cytosol and to the peroxisome in a Pex5p-dependent manner in glucose
S000005415	YOL054W	PSH1	Pob3/Spt16 Histone associated	228614	229834	1	E3 ubiquitin ligase targeting centromere-binding protein Cse4p	E3 ubiquitin ligase targeting centromere-binding protein Cse4p; mediates polyubiquitination and degradation of histone H3 variant Cse4p; ubiquitination of Cse4p may be antagonized by Scm3p; interacts with the FACT complex preventing mislocalization of Cse4p to euchromatin independent of Slx5p; formyl-methionine/N-recognin component of N-end rule pathway mediating destruction of formylated cytosolic proteins elevated in response to starvation or stationary phase entry
S000005416	YOL055C	THI20	THIamine metabolism	224420	226075	-1	Trifunctional enzyme of thiamine biosynthesis, degradation and salvage	Trifunctional enzyme of thiamine biosynthesis, degradation and salvage; has hydroxymethylpyrimidine (HMP) kinase, HMP-phosphate (HMP-P) kinase and thiaminase activities; member of a gene family with THI21 and THI22; HMP and HMP-P kinase activity redundant with Thi21p
S000005417	YOL056W	GPM3	Glycerate PhosphoMutase	223268	224179	1	Nonfunctional homolog of Gpm1p phosphoglycerate mutase	Nonfunctional homolog of Gpm1p phosphoglycerate mutase; if functional, would convert 3-phosphoglycerate to 2-phosphoglycerate in glycolysis; GPM3 has a paralog, GPM2, that arose from the whole genome duplication
S000005418	YOL057W	""	""	220767	222902	1	Dipeptidyl-peptidase III	Dipeptidyl-peptidase III; cleaves dipeptides from the amino terminus of target proteins; highly active on synthetic substrate Arg-Arg-2-naphthylamide; mammalian ortholog may be a biomarker for some cancers
S000005419	YOL058W	ARG1	ARGinine requiring	219211	220473	1	Argininosuccinate synthetase	Argininosuccinate synthetase; catalyzes the formation of L-argininosuccinate from citrulline and L-aspartate in the arginine biosynthesis pathway; potential Cdc28p substrate
S000005420	YOL059W	GPD2	Glycerol-3-Phosphate Dehydrogenase	217126	218448	1	NAD-dependent glycerol 3-phosphate dehydrogenase	NAD-dependent glycerol 3-phosphate dehydrogenase; expression is controlled by an oxygen-independent signaling pathway required to regulate metabolism under anoxic conditions; located in cytosol and mitochondria; constitutively active but is inactivated via phosphorylation by energy-stress responsive kinase SNF1; GPD2 has a paralog, GPD1, that arose from the whole genome duplication
S000005421	YOL060C	MAM3	""	214017	216137	-1	Protein involved in magnesium homeostasis	Protein involved in magnesium homeostasis; required for Mg2+ sequestration in vacuole; required for normal mitochondrial morphology; has similarity to hemolysins
S000005422	YOL061W	PRS5	PhosphoRibosylpyrophosphate Synthetase	212244	213734	1	5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase	5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase; synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes; forms cytoplasmic foci upon DNA replication stress
S000005423	YOL062C	APM4	clathrin Adaptor Protein complex Medium chain	210520	211995	-1	Cargo-binding mu subunit of AP-2	Cargo-binding mu subunit of AP-2; AP-2 is a heterotetrameric endocytic cargo-binding adaptor that facilitates uptake of membrane proteins during clathrin-mediated endocytosis; Apm4p is required for AP-2 function and localization, and binds cell wall stress receptor Mid2p; AP-2 is required for cell polarity responses to pheromone, nutritional status and cell wall damage in S. cerevisiae, and for hyphal growth in C. albicans; AP-2 complex is conserved in mammals
S000005424	YOL063C	CRT10	Constitutive RNR Transcription regulators	207392	210265	-1	Protein required for the degradation of mutant 25S rRNAs	Protein required for the degradation of mutant 25S rRNAs; functions in Mms1 pathway of nonfunctional 25S rRNA decay; expression induced by DNA damage and null mutations confer increased resistance to hydroxyurea; N-terminal region has a leucine repeat and a WD40 repeat
S000005425	YOL064C	MET22	METhionine requiring	206103	207176	-1	Bisphosphate-3'-nucleotidase	Bisphosphate-3'-nucleotidase; involved in salt tolerance and methionine biogenesis; dephosphorylates 3'-phosphoadenosine-5'-phosphate and 3'-phosphoadenosine-5'-phosphosulfate, intermediates of the sulfate assimilation pathway; human homolog BPNT1 complements yeast null mutant
S000005426	YOL065C	INP54	INositol polyphosphate 5-Phosphatase	204731	205885	-1	Phosphatidylinositol 4,5-bisphosphate 5-phosphatase	Phosphatidylinositol 4,5-bisphosphate 5-phosphatase; role in secretion; localizes to the endoplasmic reticulum via the C-terminal tail; lacks the Sac1 domain and proline-rich region found in the other 3 INP proteins
S000005427	YOL066C	RIB2	RIBoflavin biosynthesis	202696	204471	-1	Bifunctional DRAP deaminase tRNA:pseudouridine synthase	Bifunctional DRAP deaminase tRNA:pseudouridine synthase; the deaminase catalyzes the third step in riboflavin biosynthesis and the synthase catalyzes formation of pseudouridine at position 32 in cytoplasmic tRNAs; RIB2 has a paralog, PUS9, that arose from the whole genome duplication
S000005428	YOL067C	RTG1	ReTroGrade regulation	201985	202518	-1	Transcription factor (bHLH) involved in interorganelle communication	Transcription factor (bHLH) involved in interorganelle communication; contributes to communication between mitochondria, peroxisomes, and nucleus; target of Hog1p; activated in stochastic pulses of nuclear localization
S000005429	YOL068C	HST1	Homolog of SIR Two (SIR2)	200368	201879	-1	NAD(+)-dependent histone deacetylase	NAD(+)-dependent histone deacetylase; essential subunit of the Sum1p/Rfm1p/Hst1p complex required for ORC-dependent silencing and meiotic repression; non-essential subunit of the Set3C deacetylase complex; involved in telomere maintenance; HST1 has a paralog, SIR2, that arose from the whole genome duplication
S000005430	YOL069W	NUF2	NUclear Filament-containing protein	198942	200297	1	Component of the kinetochore-associated Ndc80 complex	Component of the kinetochore-associated Ndc80 complex; involved in chromosome segregation, spindle checkpoint activity, and kinetochore clustering; evolutionarily conserved; other members include Ndc80p, Nuf2p, Spc24p, and Spc25p
S000005431	YOL070C	NBA1	Nap1p and Bud neck Associated	197220	198725	-1	Protein involved in axial bud site selection	Protein involved in axial bud site selection; prevents repolarization of cells at previous division sites; recruited by Aim44p and Nap1p to the cell cortex and to cytokinesis remnants (bud scars) where it prevents Rsr1p-mediated activation of Cdc24p, the Cdc42p GEF; localizes to the cytoplasm, and the cell division site prior to being stably recruited to cytokinesis remnants; establishes the transient localization of Rga1p, a Cdc42p GAP, at bud scars along with Nis1p; potential Cdc28p substrate
S000005432	YOL071W	SDH5	Succinate DeHydrogenase	196507	196995	1	Protein required for flavinylation of Sdh1p	Protein required for flavinylation of Sdh1p; binds to Sdh1p and promotes FAD cofactor attachment, which is necessary for succinate dehydrogenase (SDH) complex assembly and activity; mutations in human ortholog PGL2 are associated with neuroendocrine tumors (paraganglioma)
S000005433	YOL072W	THP1	Tho2/Hpr1 Phenotype	194970	196337	1	Nuclear pore-associated protein	Nuclear pore-associated protein; component of TREX-2 complex (Sac3p-Thp1p-Sus1p-Cdc31p) involved in transcription elongation and mRNA export from the nucleus; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP; contains a PAM domain implicated in protein-protein binding
S000005434	YOL073C	DSC2	Defective for SREBP Cleavage	193832	194800	-1	Multi-transmembrane subunit of the DSC ubiquitin ligase complex	Multi-transmembrane subunit of the DSC ubiquitin ligase complex; similar in sequence to rhomboid pseudoproteases Der1p and UBAC2 that function in ERAD; ortholog of fission yeast dsc2
S000005435	YOL075C	""	""	189658	193542	-1	Putative ABC transporter	Putative ABC transporter
S000005436	YOL076W	MDM20	Mitochondrial Distribution and Morphology	187024	189414	1	Non-catalytic subunit of the NatB N-terminal acetyltransferase	Non-catalytic subunit of the NatB N-terminal acetyltransferase; NatB catalyzes N-acetylation of proteins with specific N-terminal sequences; involved in mitochondrial inheritance and actin assembly
S000005437	YOL077C	BRX1	Xenopus laevis Brix (Biogenesis of Ribosomes in Xenopus) homolog	185848	186723	-1	Nucleolar protein	Nucleolar protein; constituent of 66S pre-ribosomal particles; depletion leads to defects in rRNA processing and a block in the assembly of large ribosomal subunits; possesses a sigma(70)-like RNA-binding motif
S000005438	YOL078W	AVO1	Adheres VOraciously (to TOR2)	181682	185212	1	Subunit of TORC2, a regulator of plasma membrane (PM) homeostasis	Subunit of TORC2, a regulator of plasma membrane (PM) homeostasis; C-terminal PH domain binds to phosphoinositide-(4,5)-bisphosphate (PI(4,5)P2); binds directly to Ypk2p a Tor2p substrate and downstream TORC2 effector through a conserved central CRIM domain; acts with Tsc11p as a scaffold important for the integrity of the TORC2 complex; dispensable for PM localization of TORC2; role in regulating actin organization and growth control; orthologous to human MAPKAP1/SIN1
S000005439	YOL079W	""	""	181057	181455	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000005440	YOL080C	REX4	Rna EXonuclease	180558	181427	-1	Putative RNA exonuclease	Putative RNA exonuclease; possibly involved in pre-rRNA processing and ribosome assembly
S000005441	YOL081W	IRA2	Inhibitory Regulator of the RAS-cAMP pathway	171070	180309	1	GTPase-activating protein	GTPase-activating protein; negatively regulates RAS by converting it from the GTP- to the GDP-bound inactive form, required for reducing cAMP levels under nutrient limiting conditions; IRA2 has a paralog, IRA1, that arose from the whole genome duplication; defects in human homolog NF1 are associated with neurofibromatosis
S000005442	YOL082W	ATG19	AuTophaGy related	168727	169974	1	Receptor protein for the cytoplasm-to-vacuole targeting (Cvt) pathway	Receptor protein for the cytoplasm-to-vacuole targeting (Cvt) pathway; delivers cargo proteins aminopeptidase I (Ape1p) and alpha-mannosidase (Ams1p) to the phagophore assembly site for packaging into Cvt vesicles; interaction with Atg19p during the Cvt pathway requires phosphorylation by Hrr25p
S000005443	YOL083W	ATG34	AuTophaGy related	165714	166952	1	Receptor protein involved in selective autophagy during starvation	Receptor protein involved in selective autophagy during starvation; specifically involved in the transport of cargo protein alpha-mannosidase (Ams1p); Atg19p paralog
S000005444	YOL084W	PHM7	PHosphate Metabolism	162356	165331	1	Protein of unknown function	Protein of unknown function; expression is regulated by phosphate levels; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; protein abundance increases in response to DNA replication stress
S000005445	YOL085C	""	""	161673	162014	-1	Putative protein of unknown function	Putative protein of unknown function; conserved among S. cerevisiae strains; YOL085C is not an essential gene; partially overlaps dubious ORF YOL085W-A
S000005446	YOL086C	ADH1	Alcohol DeHydrogenase	159548	160594	-1	Alcohol dehydrogenase	Alcohol dehydrogenase; fermentative isozyme active as homo- or heterotetramers; required for the reduction of acetaldehyde to ethanol, the last step in the glycolytic pathway; ADH1 has a paralog, ADH5, that arose from the whole genome duplication
S000005447	YOL087C	DUF1	DUB-associated Factor	155287	158637	-1	Ubiquitin-binding protein of unknown function	Ubiquitin-binding protein of unknown function; contains one WD40 repeat in a beta-propeller fold; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; homolog of human WDR48/UAF1, which is involved in regulating the Fanconi anemia pathway; deletion mutant is sensitive to various chemicals including phenanthroline, sanguinarine, and nordihydroguaiaretic acid
S000005448	YOL088C	MPD2	Multicopy suppressor of PDI1 deletion	153912	154745	-1	Member of the protein disulfide isomerase (PDI) family	Member of the protein disulfide isomerase (PDI) family; exhibits chaperone activity; overexpression suppresses the lethality of a pdi1 deletion but does not complement all Pdi1p functions; undergoes oxidation by Ero1p
S000005449	YOL089C	HAL9	HALotolerance	150398	153490	-1	Putative transcription factor containing a zinc finger	Putative transcription factor containing a zinc finger; overexpression increases salt tolerance through increased expression of the ENA1 (Na+/Li+ extrusion pump) gene while gene disruption decreases both salt tolerance and ENA1 expression; HAL9 has a paralog, TBS1, that arose from the whole genome duplication
S000005450	YOL090W	MSH2	MutS Homolog	147382	150276	1	Protein that binds to DNA mismatches	Protein that binds to DNA mismatches; forms heterodimers with Msh3p and Msh6p that bind to DNA mismatches to initiate the mismatch repair process; contains a Walker ATP-binding motif required for repair activity and involved in interstrand cross-link repair; Msh2p-Msh6p binds to and hydrolyzes ATP; required for silencing at the silent mating-type loci and telomeres
S000005451	YOL091W	SPO21	SPOrulation	145334	147163	1	Component of the meiotic outer plaque of the spindle pole body	Component of the meiotic outer plaque of the spindle pole body; involved in modifying the meiotic outer plaque that is required prior to prospore membrane formation; SPO21 has a paralog, YSW1, that arose from the whole genome duplication
S000005452	YOL092W	YPQ1	Yeast PQ-loop protein	144204	145130	1	Putative vacuolar membrane transporter for cationic amino acids	Putative vacuolar membrane transporter for cationic amino acids; likely contributes to amino acid homeostasis by exporting cationic amino acids from the vacuole; member of the PQ-loop family, with seven transmembrane domains; similar to mammalian PQLC2 vacuolar transporter; YPQ1 has a paralog, RTC2, that arose from the whole genome duplication
S000005453	YOL093W	TRM10	Transfer RNA Methyltransferase	142815	143696	1	tRNA methyltransferase	tRNA methyltransferase; methylates the N-1 of guanine at position 9 in tRNAs; involved in the methylation of guanosine and uridine ribonucleosides in mRNA; member of the SPOUT (SpoU-TrmD) methyltransferase family; human ortholog TRMT10A plays a role in the pathogenesis of microcephaly and early onset diabetes; an 18-mer ncRNA originates from the TRM10 locus; genetic analysis shows the 18-mer ncRNA is a translational regulator; protein abundance increases in response to DNA replication stress
S000005454	YOL094C	RFC4	Replication Factor C	141584	142555	-1	Subunit of heteropentameric Replication factor C (RF-C)	Subunit of heteropentameric Replication factor C (RF-C); which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon; relocalizes to the cytosol in response to hypoxia
S000005455	YOL095C	HMI1	Helicase in MItochondria	139227	141347	-1	Mitochondrial inner membrane localized ATP-dependent DNA helicase	Mitochondrial inner membrane localized ATP-dependent DNA helicase; required for the maintenance of the mitochondrial genome; not required for mitochondrial transcription; has homology to E. coli helicase uvrD
S000005456	YOL096C	COQ3	COenzyme Q	138107	139045	-1	O-methyltransferase	O-methyltransferase; catalyzes two different O-methylation steps in ubiquinone (Coenzyme Q) biosynthesis; component of a mitochondrial ubiquinone-synthesizing complex; phosphoprotein
S000005457	YOL097C	WRS1	W (tryptophan) RS (tRNA synthetase)	136527	137825	-1	Cytoplasmic tryptophanyl-tRNA synthetase	Cytoplasmic tryptophanyl-tRNA synthetase; aminoacylates tryptophanyl-tRNA; human homolog WARS can complement yeast null mutant
S000005458	YOL098C	SDD3	Suppressor of Degenerative Death	132725	135838	-1	Putative metalloprotease	Putative metalloprotease; overproduction suppresses lethality due to expression of the dominant PET9 allele AAC2-A128P
S000005459	YOL099C	""	""	132017	132508	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps verified gene PKH2/YOL100W; may interact with ribosomes
S000005460	YOL100W	PKH2	Pkb-activating Kinase Homolog	129237	132482	1	Serine/threonine protein kinase	Serine/threonine protein kinase; involved in sphingolipid-mediated signaling pathway that controls endocytosis; activates Ypk1p and Ykr2p, components of signaling cascade required for maintenance of cell wall integrity; contains a PH-like domain; redundant with Pkh1p; PKH2 has a paralog, PKH1, that arose from the whole genome duplication
S000005461	YOL101C	IZH4	Implicated in Zinc Homeostasis	126982	127920	-1	Membrane protein involved in zinc ion homeostasis	Membrane protein involved in zinc ion homeostasis; member of the four-protein IZH family; expression induced by fatty acids and altered zinc levels; deletion reduces sensitivity to excess zinc; possible role in sterol metabolism; protein increases in abundance and relocalizes from nucleus to ER upon DNA replication stress; IZH4 has a paralog, IZH1, that arose from the whole genome duplication
S000005462	YOL102C	TPT1	tRNA 2'-PhosphoTransferase	125997	126689	-1	tRNA 2'-phosphotransferase	tRNA 2'-phosphotransferase; catalyzes the final step in tRNA splicing, transferring the 2'-PO(4) from the splice junction to NAD(+), forming ADP-ribose 1''-2''cyclic phosphate and nicotinamide; ADP-ribosyltransferase that ADP-ribosylates 5'-phosphorylated ssDNA and ssRNAs; human and mouse orthologs complement the inviability of the yeast null mutant
S000005463	YOL103W	ITR2	myo-Inositol TRansporter	124001	125830	1	Myo-inositol transporter	Myo-inositol transporter; member of the sugar transporter superfamily; expressed constitutively; ITR2 has a paralog, ITR1, that arose from the whole genome duplication
S000005464	YOL104C	NDJ1	NonDisJunction	116396	117454	-1	Protein that regulates meiotic SPB cohesion and telomere clustering	Protein that regulates meiotic SPB cohesion and telomere clustering; localizes to both spindle pole bodies (SPBs) and telomeres; required for bouquet formation, effective homolog pairing, ordered cross-over distribution, sister chromatid cohesion at meiotic telomeres, chromosomal segregation and telomere-led rapid prophase movement
S000005465	YOL105C	WSC3	cell Wall integrity and Stress response Component	114138	115808	-1	Sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway	Sensor-transducer of the stress-activated PKC1-MPK1 signaling pathway; involved in maintenance of cell wall integrity; involved in response to heat shock and other stressors; regulates 1,3-beta-glucan synthesis; WSC3 has a paralog, WSC2, that arose from the whole genome duplication
S000005466	YOL106W	""	""	113226	113579	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000005467	YOL107W	""	""	112102	113130	1	Putative protein of unknown function	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and colocalizes in a punctate pattern with the early golgi/COPI vesicles; YOL107W is not an essential protein
S000005468	YOL108C	INO4	INOsitol requiring	111431	111886	-1	Transcription factor involved in phospholipid synthesis	Transcription factor involved in phospholipid synthesis; required for derepression of inositol-choline-regulated genes involved in phospholipid synthesis; forms a complex, with Ino2p, that binds the inositol-choline-responsive element through a basic helix-loop-helix domain
S000005469	YOL109W	ZEO1	ZEOcin resistance	110297	110638	1	Peripheral membrane protein of the plasma membrane	Peripheral membrane protein of the plasma membrane; interacts with Mid2p; regulates the cell integrity pathway mediated by Pkc1p and Slt2p; the authentic protein is detected in a phosphorylated state in highly purified mitochondria
S000005470	YOL110W	SHR5	Suppressor of Hyperactive Ras	109176	109889	1	Palmitoyltransferase subunit	Palmitoyltransferase subunit; this complex adds a palmitoyl lipid moiety to heterolipidated substrates such as Ras1p and Ras2p through a thioester linkage; palmitoylation is required for Ras2p membrane localization; Palmitoyltransferase is composed of Shr5p and Erf2
S000005471	YOL111C	MDY2	Mating-Deficient Yeast	108258	108896	-1	Protein involved in inserting tail-anchored proteins into ER membranes	Protein involved in inserting tail-anchored proteins into ER membranes; forms a complex with Get4p; required for efficient mating; involved in shmoo formation and nuclear migration in the pre-zygote; associates with ribosomes
S000005472	YOL112W	MSB4	Multicopy Suppression of a Budding defect	106710	108188	1	GTPase-activating protein of the Ras superfamily	GTPase-activating protein of the Ras superfamily; acts primarily on Sec4p, localizes to the bud site and bud tip; msb3 msb4 double mutation causes defects in secretion and actin organization; similar to the TBC-domain Tre2 oncogene; MSB4 has a paralog, MSB3, that arose from the whole genome duplication; human homolog USP6NL can complement yeast msb3 msb4 double null mutant
S000005473	YOL113W	SKM1	STE20/PAK homologous Kinase related to Morphogenesis	104326	106293	1	Member of the PAK family of serine/threonine protein kinases	Member of the PAK family of serine/threonine protein kinases; similar to Ste20p; involved in down-regulation of sterol uptake; proposed to be a downstream effector of Cdc42p during polarized growth; SKM1 has a paralog, CLA4, that arose from the whole genome duplication
S000005474	YOL114C	PTH4	Peptidyl tRNA Hydrolase	103317	103925	-1	Stalled-ribosome rescue factor srRF1	Stalled-ribosome rescue factor srRF1; similar to human peptidyl-tRNA hydrolase ICT1; associates with mitochondrial large subunit; not an essential gene
S000005475	YOL115W	PAP2	Poly(A) Polymerase	101475	103229	1	Non-canonical poly(A) polymerase	Non-canonical poly(A) polymerase; involved in nuclear RNA degradation as a component of TRAMP; catalyzes polyadenylation of hypomodified tRNAs, and snoRNA and rRNA precursors; required for mRNA surveillance and maintenance of genome integrity, serving as a link between RNA and DNA metabolism; overlapping but non-redundant functions with Trf5p; relocalizes to cytosol in response to hypoxia
S000005476	YOL116W	MSN1	Multicopy suppressor of SNF1 mutation	99809	100957	1	Transcriptional activator	Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress
S000005477	YOL117W	RRI2	""	97551	99488	1	Subunit of the COP9 signalosome (CSN) complex	Subunit of the COP9 signalosome (CSN) complex; this complex cleaves the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; plays a role in the mating pheromone response
S000005478	YOL118C	""	""	96608	96916	-1	Putative protein of unknown function	Putative protein of unknown function; conserved across S. cerevisiae strains
S000005479	YOL119C	MCH4	MonoCarboxylate transporter Homolog	94856	96361	-1	Protein with similarity to mammalian monocarboxylate permeases	Protein with similarity to mammalian monocarboxylate permeases; monocarboxylate permeases are involved in transport of monocarboxylic acids across the plasma membrane but mutant is not deficient in monocarboxylate transport
S000005481	YOL121C	RPS19A	Ribosomal Protein of the Small subunit	92026	92850	-1	Protein component of the small (40S) ribosomal subunit	Protein component of the small (40S) ribosomal subunit; required for assembly and maturation of pre-40 S particles; homologous to mammalian ribosomal protein S19, no bacterial homolog; mutations in human RPS19 are associated with Diamond Blackfan anemia; RPS19A has a paralog, RPS19B, that arose from the whole genome duplication
S000005482	YOL122C	SMF1	Suppressor of Mitochondria import Function	89692	91419	-1	Divalent metal ion transporter	Divalent metal ion transporter; broad specificity for di-valent and tri-valent metals; post-translationally regulated by levels of metal ions; member of the Nramp family of metal transport proteins
S000005483	YOL123W	HRP1	Heterogenous nuclear RibonucleoProtein	87844	89448	1	Subunit of cleavage factor I	Subunit of cleavage factor I; cleavage factor I is a five-subunit complex required for the cleavage and polyadenylation of pre-mRNA 3' ends; RRM-containing heteronuclear RNA binding protein and hnRNPA/B family member that binds to poly (A) signal sequences; required for genome stability; plays a role in copper homeostasis
S000005484	YOL124C	TRM11	TRna Methyltransferase	85456	86757	-1	Catalytic subunit of adoMet-dependent tRNA methyltransferase complex	Catalytic subunit of adoMet-dependent tRNA methyltransferase complex; required for the methylation of the guanosine nucleotide at position 10 (m2G10) in tRNAs; involved in the methylation of guanosine and uridine ribonucleosides in mRNA; contains a THUMP domain and a methyltransferase domain; methyltransferase complex also contains Trm112p
S000005485	YOL125W	TRM13	TRna Methyltransferase	83834	85264	1	2'-O-methyltransferase	2'-O-methyltransferase; responsible for modification of tRNA at position 4; C-terminal domain has similarity to Rossmann-fold (RFM) superfamily of RNA methyltransferases
S000005486	YOL126C	MDH2	Malate DeHydrogenase	81787	82920	-1	Cytoplasmic malate dehydrogenase	Cytoplasmic malate dehydrogenase; one of three isozymes that catalyze interconversion of malate and oxaloacetate; involved in the glyoxylate cycle and gluconeogenesis during growth on two-carbon compounds; interacts with Pck1p and Fbp1; mutation in human homolog MDH2 causes early-onset severe encephalopathy
S000005488	YOL128C	YGK3	Yeast homolog of Glycogen synthase Kinase	78352	79479	-1	Protein kinase related to mammalian GSK-3 glycogen synthase kinases	Protein kinase related to mammalian GSK-3 glycogen synthase kinases; GSK-3 homologs (Mck1p, Rim11p, Mrk1p, Ygk3p) are involved in control of Msn2p-dependent transcription of stress responsive genes and in protein degradation; YGK3 has a paralog, MCK1, that arose from the whole genome duplication
S000005489	YOL129W	VPS68	Vacuolar Protein Sorting	77560	78114	1	Vacuolar membrane protein of unknown function	Vacuolar membrane protein of unknown function; involved in vacuolar protein sorting; also detected in the mitochondria
S000005490	YOL130W	ALR1	ALuminum Resistance	74400	76979	1	Plasma membrane Mg(2+) transporter	Plasma membrane Mg(2+) transporter; expression and turnover are regulated by Mg(2+) concentration; overexpression confers increased tolerance to Al(3+) and Ga(3+) ions; magnesium transport defect of the null mutant is functionally complemented by either of the human genes MAGT1 and TUSC3 that are not orthologous to ALR1
S000005491	YOL131W	""	""	73031	73357	1	Putative protein of unknown function	Putative protein of unknown function; YOL131W has a paralog, STB1, that arose from the whole genome duplication
S000005492	YOL132W	GAS4	Glycophospholipid-Anchored Surface protein	71300	72715	1	1,3-beta-glucanosyltransferase	1,3-beta-glucanosyltransferase; involved with Gas2p in spore wall assembly; has similarity to Gas1p; localizes to the cell wall
S000005493	YOL133W	HRT1	High level expression Reduces Ty3 transposition	70325	70690	1	RING-H2 domain core subunit of multiple ubiquitin ligase complexes	RING-H2 domain core subunit of multiple ubiquitin ligase complexes; subunit of Skp1-Cullin-F-box (SCF) that tethers the Cdc34p (E2) and Cdc53p (cullin) SCF subunits, and is required for degradation of Gic2p, Far1p, Sic1p and Cln2p; subunit of the Rtt101p-Mms1p-Mms22p ubiquitin ligase that stabilizes replication forks after DNA lesions; subunit of the Cul3p-Elc1p-Ela1p ubiquitin ligase involved in Rpb1p degradation as part of transcription-coupled repair
S000005494	YOL134C	""	""	70156	70545	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps HRT1, a verified gene that encodes an SCF ubiquitin ligase subunit
S000005495	YOL135C	MED7	MEDiator complex	69376	70044	-1	Subunit of the RNA polymerase II mediator complex	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
S000005496	YOL136C	PFK27	6-PhosphoFructo-2-Kinase	67561	68754	-1	6-phosphofructo-2-kinase	6-phosphofructo-2-kinase; catalyzes synthesis of fructose-2,6-bisphosphate; inhibited by phosphoenolpyruvate and sn-glycerol 3-phosphate, expression induced by glucose and sucrose, transcriptional regulation involves protein kinase A
S000005497	YOL137W	BSC6	Bypass of Stop Codon	65621	67114	1	Protein of unknown function with 8 putative transmembrane segments	Protein of unknown function with 8 putative transmembrane segments; ORF exhibits genomic organization compatible with a translational readthrough-dependent mode of expression
S000005498	YOL138C	RTC1	Restriction of Telomere Capping	61325	65350	-1	Subunit of SEACAT, a subcomplex of the SEA complex	Subunit of SEACAT, a subcomplex of the SEA complex; Rtc1p, along with Mtc5p and Sea4p, redundantly inhibit the TORC1 inhibitory role of the Iml1p/SEACIT (Iml1p-Npr2p-Npr3p) subcomplex, a GAP for GTPase Gtr1p (EGOC subunit) in response to amino acid limitation, thereby resulting in activation of TORC1 signaling; SEA is a coatomer-related complex that associates dynamically with the vacuole; has N-terminal WD-40 repeats and a C-terminal RING motif; null suppresses cdc13-1
S000005499	YOL139C	CDC33	Cell Division Cycle	60383	61024	-1	Translation initiation factor eIF4E	Translation initiation factor eIF4E; mRNA cap binding protein and subunit of the mRNA cap-binding complex (eIF4F) along with eIF4A (Tif1p/Tif2p) and eIF4G (Tif4631p/Tif4632p), that mediates cap-dependent translation; required with eIF4G for Ded1p-mediated stimulation of mRNA recruitment during 48S preinitiation complex (PIC) assembly; protein abundance increases in response to DNA replication stress; human homolog EIF4E can complement the cdc33 null mutant
S000005500	YOL140W	ARG8	ARGinine requiring	58759	60030	1	Acetylornithine aminotransferase	Acetylornithine aminotransferase; catalyzes the fourth step in the biosynthesis of the arginine precursor ornithine
S000005501	YOL141W	PPM2	Protein Phosphatase Methyltransferase	56452	58539	1	AdoMet-dependent tRNA methyltransferase	AdoMet-dependent tRNA methyltransferase; also involved in methoxycarbonylation; required for the synthesis of wybutosine (yW), a modified guanosine found at the 3'-position adjacent to the anticodon of phe-tRNA; similarity to Ppm1p
S000005503	YOL143C	RIB4	RIBoflavin biosynthesis	54595	55104	-1	Lumazine synthase (DMRL synthase)	Lumazine synthase (DMRL synthase); catalyzes synthesis of immediate precursor to riboflavin; DMRL synthase stands for 6,7-dimethyl-8-ribityllumazine synthase
S000005504	YOL144W	NOP8	NucleOlar Protein	53098	54552	1	Nucleolar protein required for 60S ribosomal subunit biogenesis	Nucleolar protein required for 60S ribosomal subunit biogenesis
S000005505	YOL145C	CTR9	Cln Three (CLN3) Requiring	49555	52788	-1	Component of the Paf1p complex involved in transcription elongation	Component of the Paf1p complex involved in transcription elongation; binds to and modulates the activity of RNA polymerases I and II; required for expression of a subset of genes, including cyclin genes; involved in SER3 repression by helping to maintain SRG1 transcription-dependent nucleosome occupancy; contains TPR repeats
S000005506	YOL146W	PSF3	Partner of Sld Five	48864	49448	1	Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p)	Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p); complex is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
S000005507	YOL147C	PEX11	PEroXin	47933	48643	-1	Peroxisomal protein required for medium-chain fatty acid oxidation	Peroxisomal protein required for medium-chain fatty acid oxidation; also required for peroxisome proliferation, possibly by inducing membrane curvature; localization regulated by phosphorylation; transcription regulated by Adr1p and Pip2p-Oaf1p
S000005508	YOL148C	SPT20	SuPpressor of Ty	45760	47574	-1	Subunit of the SAGA transcriptional regulatory complex	Subunit of the SAGA transcriptional regulatory complex; involved in maintaining the integrity of the complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay
S000005509	YOL149W	DCP1	mRNA DeCaPping	44938	45633	1	Subunit of the Dcp1p-Dcp2p decapping enzyme complex	Subunit of the Dcp1p-Dcp2p decapping enzyme complex; decapping complex removes the 5' cap structure from mRNAs prior to their degradation; enhances the activity of catalytic subunit Dcp2p; regulated by DEAD box protein Dhh1p; forms cytoplasmic foci upon DNA replication stress
S000005510	YOL150C	""	""	44473	44784	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000005511	YOL151W	GRE2	Genes de Respuesta a Estres (stress responsive genes)	43694	44722	1	3-methylbutanal reductase and NADPH-dependent methylglyoxal reductase	3-methylbutanal reductase and NADPH-dependent methylglyoxal reductase; stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway; restores resistance to glycolaldehyde by coupling reduction of glycolaldehyde to ethylene glycol and oxidation of NADPH to NADP+; protein abundance increases in response to DNA replication stress; methylglyoxal reductase (NADPH-dependent) is also known as D-lactaldehyde dehydrogenase
S000005512	YOL152W	FRE7	Ferric REductase	40748	42610	1	Putative ferric reductase with similarity to Fre2p	Putative ferric reductase with similarity to Fre2p; expression induced by low copper levels
S000005513	YOL153C	""	""	36822	38567	-1	Blocked reading frame, contains two in-frame stops in translation	Blocked reading frame, contains two in-frame stops in translation; this ORF is conserved, without the stops, in Saccharomyces paradoxus, S. mikatae, and S. bayanus; RNA sequencing data indicate the presence of a transcription unit at this reading frame
S000005514	YOL154W	ZPS1	Zinc- and pH-regulated Surface protein	34658	35407	1	Putative GPI-anchored protein	Putative GPI-anchored protein; transcription is induced under low-zinc conditions, as mediated by the Zap1p transcription factor, and at alkaline pH
S000005515	YOL155C	HPF1	Haze Protective Factor	28703	31606	-1	Haze-protective mannoprotein	Haze-protective mannoprotein; reduces particle size of aggregated proteins in white wines, thereby decreasing turbidity; intragenic repeat expansion controls chronological aging; expansion of intragenic tandem repeats within N-terminus sufficient to cause pronounced life span shortening; repeat expansion shifts cells from sedentary to buoyant, thereby increasing exposure to oxygen, altering methionine, lipid, and purine metabolism
S000005518	YOL158C	ENB1	ENteroBactin	19491	21311	-1	Ferric enterobactin transmembrane transporter	Ferric enterobactin transmembrane transporter; expressed under conditions of iron deprivation; member of the major facilitator superfamily; expression is regulated by Rcs1p and affected by chloroquine treatment
S000005519	YOL159C	CSS3	Condition Specific Secretion	17281	17796	-1	Protein of unknown function, secreted when constitutively expressed	Protein of unknown function, secreted when constitutively expressed; SWAT-GFP, seamless-GFP and mCherry fusion proteins localize to the cell periphery, SWAT-GFP fusion also localizes to the extracellular region, and mCherry fusion also localizes to the vacuole; deletion mutants are viable and have elevated levels of Ty1 retrotransposition and Ty1 cDNA
S000005520	YOL160W	""	""	14313	14654	1	Putative protein of unknown function	Putative protein of unknown function; conserved across S. cerevisiae strains
S000005522	YOL162W	""	""	10119	10766	1	Putative protein of unknown function	Putative protein of unknown function; proposed to be an inactive evolutionary remnant that along with neighboring gene YOL163W may have constituted a second sulfonate transporter and SOA1 paralog that is intact in S. uvarum, S. eubayanus and S. arboricola and named SOA2; member of the Dal5p subfamily of the major facilitator family
S000005523	YOL163W	""	""	9597	10106	1	Putative protein of unknown function	Putative protein of unknown function; proposed to be an inactive evolutionary remnant that along with neighboring gene YOL162W may have constituted a second sulfonate transporter and SOA1 paralog that is intact in S. uvarum, S. eubayanus and S. arboricola and named SOA2; member of the Dal5p subfamily of the major facilitator family
S000005524	YOL164W	BDS1	Bacterially Derived Sulfatase	6175	8115	1	Bacterially-derived sulfatase	Bacterially-derived sulfatase; required for use of alkyl sulfates as sulfur source
S000005527	YOR001W	RRP6	Ribosomal RNA Processing	326832	329033	1	Nuclear exosome exonuclease component	Nuclear exosome exonuclease component; has 3'-5' exonuclease activity that is regulated by Lrp1p; involved in RNA processing, maturation, surveillance, degradation, tethering, and export; role in sn/snoRNAs precursor degradation; forms a stable heterodimer with Lrp1p; has similarity to E. coli RNase D and to human PM-Sc1 100 (EXOSC10); mutant displays reduced transcription elongation in the G-less-based
S000005528	YOR002W	ALG6	Asparagine-Linked Glycosylation	329417	331051	1	ER-luminal alpha 1,3 glucosyltransferase	ER-luminal alpha 1,3 glucosyltransferase; involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; C998T transition in human ortholog ALG6 causes carbohydrate-deficient glycoprotein syndrome type-Ic; wild-type human ortholog ALG6 can partially complement yeast alg6 mutant
S000005529	YOR003W	YSP3	Yeast Subtilisin-like Protease III	331455	332891	1	Putative precursor of the subtilisin-like protease III	Putative precursor of the subtilisin-like protease III; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum; YSP3 has a paralog, PRB1, that arose from the whole genome duplication
S000005530	YOR004W	UTP23	U Three-associated Protein	333592	334356	1	Component of the small subunit processome	Component of the small subunit processome; involved in 40S ribosomal subunit biogenesis; interacts with snR30 and is required for dissociation of snR30 from large pre-ribosomal particles; has homology to PINc domain protein Fcf1p, although the PINc domain of Utp23p is not required for function; essential protein
S000005531	YOR005C	DNL4	DNA Ligase	334509	337343	-1	DNA ligase required for nonhomologous end-joining (NHEJ)	DNA ligase required for nonhomologous end-joining (NHEJ); forms stable heterodimer with required cofactor Lif1p, interacts with Nej1p; involved in meiosis, not essential for vegetative growth; mutations in human ortholog lead to ligase IV syndrome and Dubowitz syndrome
S000005532	YOR006C	TSR3	Twenty S rRNA accumulation	337680	338621	-1	Protein required for 20S pre-rRNA processing	Protein required for 20S pre-rRNA processing; involved in processing of the 20S pre-rRNA at site D to generate mature 18S rRNA; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus; relative distribution to the nucleus increases upon DNA replication stress; also detected in peroxisomes
S000005533	YOR007C	SGT2	Small Glutamine-rich Tetratricopeptide repeat-containing protein	338938	339978	-1	Glutamine-rich cytoplasmic cochaperone	Glutamine-rich cytoplasmic cochaperone; serves as a scaffold bringing together Get4, Get5p, and other TRC complex members that are required to mediate posttranslational insertion of tail-anchored proteins into the ER membrane; interacts with the prion domain of Sup35p; amyloid sensor; plays a role in targeting chaperones to prion aggregates; similar to human cochaperone SGT; forms cytoplasmic foci upon DNA replication stress
S000005534	YOR008C	SLG1	Synthetic Lethal with Gap	341278	342414	-1	Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway	Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response
S000005535	YOR009W	TIR4	TIp1-Related	344335	345798	1	Cell wall mannoprotein	Cell wall mannoprotein; expressed under anaerobic conditions and required for anaerobic growth; transcription is also induced by cold shock; member of the Srp1p/Tip1p family of serine-alanine-rich proteins
S000005536	YOR010C	TIR2	TIp1-Related	346195	346950	-1	Putative cell wall mannoprotein	Putative cell wall mannoprotein; member of the Srp1p/Tip1p family of serine-alanine-rich proteins; transcription is induced by cold shock and anaerobiosis; TIR2 has a paralog, TIR3, that arose from the whole genome duplication
S000005537	YOR011W	AUS1	ABC protein involved in Uptake of Sterols	349679	353863	1	Plasma membrane sterol transporter of the ATP-binding cassette family	Plasma membrane sterol transporter of the ATP-binding cassette family; required, along with Pdr11p, for uptake of exogenous sterols and their incorporation into the plasma membrane; activity is stimulated by phosphatidylserine; sterol uptake is required for anaerobic growth because sterol biosynthesis requires oxygen; AUS1 has a paralog, PDR11, that arose from the whole genome duplication
S000005538	YOR012W	""	""	356543	356956	1	Putative protein of unknown function	Putative protein of unknown function
S000005539	YOR013W	IRC11	Increased Recombination Centers	356751	357221	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YOR012W; null mutant displays increased levels of spontaneous Rad52 foci
S000005540	YOR014W	RTS1	Rox Three Suppressor	357674	359947	1	B-type regulatory subunit of protein phosphatase 2A (PP2A)	B-type regulatory subunit of protein phosphatase 2A (PP2A); Rts1p and Cdc55p are alternative regulatory subunits for PP2A catalytic subunits, Pph21p and Pph22p; PP2A-Rts1p protects cohesin when recruited by Sgo1p to the pericentromere; highly enriched at centromeres in the absence of Cdc55p; required for maintenance of septin ring organization during cytokinesis, for ring disassembly in G1 and for dephosphorylation of septin, Shs1p; homolog of the mammalian B' subunit of PP2A
S000005541	YOR015W	""	""	359993	360352	1	Putative protein of unknown function	Putative protein of unknown function; conserved among S. cerevisiae strains; YOR015W is not an essential gene
S000005542	YOR016C	ERP4	Emp24p/Erv25p Related Protein	360461	361084	-1	Member of the p24 family involved in ER to Golgi transport	Member of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; ERP4 has a paralog, ERP2, that arose from the whole genome duplication
S000005543	YOR017W	PET127	PETite colonies	361412	363814	1	Protein with a role in 5'-end processing of mitochondrial RNAs	Protein with a role in 5'-end processing of mitochondrial RNAs; located in the mitochondrial membrane
S000005544	YOR018W	ROD1	Resistance to O-Dinitrobenzene	364369	366882	1	Alpha-arrestin involved in ubiquitin-dependent endocytosis	Alpha-arrestin involved in ubiquitin-dependent endocytosis; activating dephosphorylation relays glucose signaling to transporter endocytosis; calcineurin dephosphorylation is required for Rsp5p-dependent internalization of agonist-occupied Ste2p, as part of signal desensitization; recruits Rsp5p to Ste2p via its 2 PPXY motifs; protein abundance increases in response to DNA replication stress; ROD1 has a paralog, ROG3, that arose from the whole genome duplication
S000005545	YOR019W	""	""	368127	370319	1	Protein of unknown function	Protein of unknown function; may interact with ribosomes, based on co-purification experiments; YOR019W has a paralog, JIP4, that arose from the whole genome duplication
S000005546	YOR020C	HSP10	Heat Shock Protein	370524	370844	-1	Mitochondrial matrix co-chaperonin	Mitochondrial matrix co-chaperonin; inhibits the ATPase activity of Hsp60p, a mitochondrial chaperonin; involved in protein folding and sorting in the mitochondria; 10 kD heat shock protein with similarity to E. coli groES
S000005547	YOR021C	SFM1	Spout Family Methyltransferase	372798	373439	-1	SPOUT methyltransferase	SPOUT methyltransferase; catalyzes omega-monomethylation of Rps3p on Arg-146; not an essential gene; predicted to be involved in rRNA processing and ribosome biogenesis and in biopolymer catabolism
S000005548	YOR022C	DDL1	DDHD Domain-containing Lipase	373710	375857	-1	DDHD domain-containing phospholipase A1	DDHD domain-containing phospholipase A1; mitochondrial matrix enzyme with sn-1-specific activity, hydrolyzing cardiolipin, PE, PC, PG and PA; implicated in remodeling of mitochondrial phospholipids; antagonistically regulated by Aft1p and Aft2p; in humans, mutations in DDHD1 and DDHD2 genes cause specific types of hereditary spastic paraplegia, while DDL1-defective yeast share similar phenotypes such as mitochondrial dysfunction and defects in lipid metabolism
S000005549	YOR023C	AHC1	Ada Histone acetyltransferase complex Component	376012	377712	-1	Subunit of the Ada histone acetyltransferase complex	Subunit of the Ada histone acetyltransferase complex; required for structural integrity of the complex; Ahc2p and Ahc1p are unique to the ADA complex and not shared with the related SAGA and SLIK complexes; Ahc2p may tether Ahc1p to the complex
S000005550	YOR024W	""	""	377847	378170	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000005551	YOR025W	HST3	Homolog of SIR Two (SIR2)	378219	379562	1	Sirtuin, histone H3 deacetylase	Sirtuin, histone H3 deacetylase; involved along with Hst4p in telomeric silencing, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism; member of the Sir2 family of NAD(+)-dependent protein deacetylases
S000005552	YOR026W	BUB3	Budding Uninhibited by Benzimidazole	379781	380806	1	Kinetochore checkpoint WD40 repeat protein	Kinetochore checkpoint WD40 repeat protein; localizes to kinetochores during prophase and metaphase, delays anaphase in the presence of unattached kinetochores; forms complexes with Mad1p-Bub1p and with Cdc20p, binds Mad2p and Mad3p; functions at kinetochore to activate APC/C-Cdc20p for normal mitotic progression
S000005553	YOR027W	STI1	STress Inducible	381053	382822	1	Evolutionarily-conserved Hsp90 cochaperone	Evolutionarily-conserved Hsp90 cochaperone; regulates spatial organization of amyloid-like proteins in the cytosol, thereby buffering the proteotoxicity caused by amyloid-like proteins; interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop
S000005554	YOR028C	CIN5	Chromosome INstability	383533	384420	-1	Basic leucine zipper (bZIP) transcription factor of the yAP-1 family	Basic leucine zipper (bZIP) transcription factor of the yAP-1 family; physically interacts with the Tup1-Cyc8 complex and recruits Tup1p to its targets; mediates pleiotropic drug resistance and salt tolerance; nuclearly localized under oxidative stress and sequestered in the cytoplasm by Lot6p under reducing conditions; CIN5 has a paralog, YAP6, that arose from the whole genome duplication
S000005555	YOR029W	""	""	384600	384935	1	Putative protein of unknown function	Putative protein of unknown function; conserved among S. cerevisiae strains; YOR029W is not an essential gene
S000005556	YOR030W	DFG16	Defective for Filamentous Growth	386825	388684	1	Probable multiple transmembrane protein	Probable multiple transmembrane protein; involved in diploid invasive and pseudohyphal growth upon nitrogen starvation; is glycosylated and phosphorylated; interacts with Rim21p and Rim9p in the plasma membrane to form a pH-sensing complex in the Rim101 pathway and is required to maintain Rim21p levels; required for accumulation of processed Rim101p
S000005557	YOR031W	CRS5	Copper-Resistant Suppressor	389213	389422	1	Copper-binding metallothionein	Copper-binding metallothionein; required for wild-type copper resistance
S000005558	YOR032C	HMS1	High-copy Mep Suppressor	389771	391075	-1	bHLH protein with similarity to myc-family transcription factors	bHLH protein with similarity to myc-family transcription factors; overexpression confers hyperfilamentous growth and suppresses the pseudohyphal filamentation defect of a diploid mep1 mep2 homozygous null mutant
S000005559	YOR033C	EXO1	EXOnuclease	392416	394524	-1	5'-3' exonuclease and flap-endonuclease	5'-3' exonuclease and flap-endonuclease; involved in recombination, double-strand break repair, MMS2 error-free branch of the post replication (PRR) pathway and DNA mismatch repair; role in telomere maintenance; member of the Rad2p nuclease family, with conserved N and I nuclease domains; relative distribution to the nucleus increases upon DNA replication stress; EXO1 has a paralog, DIN7, that arose from the whole genome duplication
S000005560	YOR034C	AKR2	AnKyrin Repeat-containing protein	394837	397086	-1	Ankyrin repeat-containing protein	Ankyrin repeat-containing protein; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; possibly involved in constitutive endocytosis of Ste3p; AKR2 has a paralog, AKR1, that arose from the whole genome duplication
S000005561	YOR035C	SHE4	Swi5p-dependent HO Expression	397735	400104	-1	Protein containing a UCS (UNC-45/CRO1/SHE4) domain	Protein containing a UCS (UNC-45/CRO1/SHE4) domain; binds to myosin motor domains to regulate myosin function; involved in endocytosis, polarization of the actin cytoskeleton, and asymmetric mRNA localization
S000005562	YOR036W	PEP12	carboxyPEPtidase Y-deficient	400348	401214	1	Target membrane receptor (t-SNARE)	Target membrane receptor (t-SNARE); for vesicular intermediates traveling between the Golgi apparatus and the vacuole; controls entry of biosynthetic, endocytic, and retrograde traffic into the prevacuolar compartment; syntaxin
S000005563	YOR037W	CYC2	CYtochrome C	401555	402655	1	Mitochondrial peripheral inner membrane protein	Mitochondrial peripheral inner membrane protein; contains a FAD cofactor in a domain exposed in the intermembrane space; exhibits redox activity in vitro; likely participates in ligation of heme to acytochromes c and c1 (Cyc1p and Cyt1p)
S000005564	YOR038C	HIR2	HIstone Regulation	402761	405388	-1	Subunit of HIR nucleosome assembly complex	Subunit of HIR nucleosome assembly complex; involved in regulation of histone gene transcription; recruits Swi-Snf complexes to histone gene promoters; promotes heterochromatic gene silencing with Asf1p; relocalizes to the cytosol in response to hypoxia
S000005565	YOR039W	CKB2	Casein Kinase Beta' subunit	405768	406544	1	Beta' regulatory subunit of casein kinase 2 (CK2)	Beta' regulatory subunit of casein kinase 2 (CK2); a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase
S000005566	YOR040W	GLO4	GLyOxalase	407064	407921	1	Mitochondrial glyoxalase II	Mitochondrial glyoxalase II; catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate; GLO4 has a paralog, GLO2, that arose from the whole genome duplication
S000005567	YOR041C	""	""	408358	408789	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the uncharacterized gene CUE5; deletion in cyr1 mutant results in loss of stress resistance
S000005568	YOR042W	CUE5	Coupling of Ubiquitin conjugation to ER degradation	408425	409660	1	Selective autophagy receptor involved in aggrephagy and proteaphagy	Selective autophagy receptor involved in aggrephagy and proteaphagy; ubiquitin (Ub)-binding protein that functions as a Ub-Atg8p adaptor in Ub-dependent autophagy; serves as a proteaphagy receptor for inactivate 26S proteasomes; contains a CUE domain that binds K48-and K63-linked Ub chains and monoubiquitin, facilitating intramolecular monoubiquitination; human TOLLIP is a functional CUE-domain homolog, that complements a null mutant, rescuing the hypersensitivity of the null mutant to Htt-96Q
S000005569	YOR043W	WHI2	WHIskey	410870	412330	1	Negative regulator of TORC1 in response to limiting leucine	Negative regulator of TORC1 in response to limiting leucine; suppresses TORC1 activity with binding partners Psr1p/Psr2p, acting in parallel with SEACIT; regulates cell cycle arrest in stationary phase; inhibits Ras-cAMP-PKA regulation of apoptosis during nutrient depletion; required with Psr1p for activation of the general stress response; role in rapamycin-induced mitophagy; localizes to the cell periphery; human tumor suppressor and Whi2-like protein KCTD11 functionally complements the null
S000005570	YOR044W	IRC23	Increased Recombination Centers	413007	413480	1	Protein of unknown function involved in multidrug resistance	Protein of unknown function involved in multidrug resistance; green fluorescent protein (GFP)-fusion localizes to the ER; null mutant displays increased levels of spontaneous Rad52p foci; IRC23 has a paralog, BSC2, that arose from the whole genome duplication
S000005571	YOR045W	TOM6	Translocase of the Outer Mitochondrial membrane	413852	414037	1	Component of the TOM (translocase of outer membrane) complex	Component of the TOM (translocase of outer membrane) complex; responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex
S000005572	YOR046C	DBP5	Dead Box Protein	414459	415907	-1	Cytoplasmic ATP-dependent RNA helicase of the DEAD-box family	Cytoplasmic ATP-dependent RNA helicase of the DEAD-box family; involved in mRNA export from the nucleus, remodeling messenger ribonucleoprotein particles (mRNPs), with ATPase activity stimulated by Gle1p, IP6 and Nup159p; involved in translation termination along with Sup45p (eRF1); role in the cellular response to heat stress
S000005573	YOR047C	STD1	Suppressor of Tbp Deletion	416347	417681	-1	Protein involved in control of glucose-regulated gene expression	Protein involved in control of glucose-regulated gene expression; interacts with kinase Snf1p, glucose sensors Snf3p and Rgt2p, TATA-binding Spt15p; regulator of transcription factor Rgt1p; interactions with Pma1p appear to propagate [GAR+]; STD1 has a paralog, MTH1, that arose from the whole genome duplication
S000005574	YOR048C	RAT1	Ribonucleic Acid Trafficking	418630	421650	-1	Nuclear 5' to 3' single-stranded RNA exonuclease	Nuclear 5' to 3' single-stranded RNA exonuclease; involved in RNA metabolism, including rRNA and snoRNA processing, as well as poly (A+) dependent and independent mRNA transcription termination; required for cotranscriptional pre-rRNA cleavage; displaces Cdk1p from elongating transcripts, especially as RNAPII reaches the poly(A) site, negatively regulates phosphorylation of the CTD of RNAPII, and inhibits RNAPII transcriptional elongation
S000005575	YOR049C	RSB1	Resistance to Sphingoid long-chain Base	422668	423732	-1	Sphingoid long-chain base (LCB) efflux transporter	Sphingoid long-chain base (LCB) efflux transporter; integral membrane transporter that localizes to the plasma membrane and may transport long chain bases (LCBs) from the cytoplasmic side toward the extracytoplasmic side of the membrane; role in glycerophospholipid translocation; suppressor of the sphingoid LCB sensitivity of an LCB-lyase mutation
S000005576	YOR050C	""	""	424270	424617	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; null mutant is viable
S000005577	YOR051C	ETT1	Enhancer of Translation Termination	424846	426084	-1	Nuclear protein that inhibits replication of Brome mosaic virus	Nuclear protein that inhibits replication of Brome mosaic virus; S. cerevisiae is a model system for studying replication of positive-strand RNA viruses in their natural hosts; deletion increases stop codon readthrough
S000005578	YOR052C	TMC1	Trivalent Metalloid sensitive, Cuz1-related protein	426772	427224	-1	AN1-type zinc finger protein, effector of proteotoxic stress response	AN1-type zinc finger protein, effector of proteotoxic stress response; stress-inducible transcriptional target of Rpn4p; induced by nitrogen limitation, weak acid, misfolded proteins; short-lived protein, degraded by proteasome; may protect cells from trivalent metalloid induced proteotoxicity; contains PACE promoter element; ortholog of human AIRAP, which stimulates proteasome activity in response to arsenic; protein abundance increases under DNA replication stress
S000005579	YOR053W	""	""	427817	428158	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VHS3/YOR054C
S000005580	YOR054C	VHS3	Viable in a Hal3 Sit4 background	427833	429857	-1	Negative regulatory subunit of protein phosphatase 1 Ppz1p	Negative regulatory subunit of protein phosphatase 1 Ppz1p; involved in coenzyme A biosynthesis; subunit of the phosphopantothenoylcysteine decarboxylase (PPCDC; Cab3p, Sis2p, Vhs3p) complex and the CoA-Synthesizing Protein Complex (CoA-SPC: Cab2p, Cab3p, Cab4p, Cab5p, Sis2p and Vhs3p)
S000005581	YOR055W	""	""	429478	429912	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000005582	YOR056C	NOB1	Nin1 (One) Binding protein	430247	431626	-1	Protein involved in proteasomal and 40S ribosomal subunit biogenesis	Protein involved in proteasomal and 40S ribosomal subunit biogenesis; required for cleavage of the 20S pre-rRNA to generate the mature 18S rRNA; cleavage is activated by Fun12p, a GTPase and translation initiation factor; relocalizes from nucleus to nucleolus upon DNA replication stress
S000005583	YOR057W	SGT1	Suppressor of G2 (Two) allele of skp1	432186	433373	1	Cochaperone protein	Cochaperone protein; regulates activity of adenylyl cyclase Cyr1p; involved in kinetochore complex assembly; associates with the SCF (Skp1p/Cdc53p/F box protein) ubiquitin ligase complex; acts as a linker between Skp1p and HSP90 complexes; protein abundance increases in response to DNA replication stress
S000005584	YOR058C	ASE1	Anaphase Spindle Elongation	433688	436345	-1	Mitotic spindle midzone-localized microtubule bundling protein	Mitotic spindle midzone-localized microtubule bundling protein; microtubule-associated protein (MAP) family member; required for spindle elongation and stabilization; undergoes cell cycle-regulated degradation by anaphase promoting complex; potential Cdc28p substrate; relative distribution to microtubules decreases upon DNA replication stress
S000005585	YOR059C	LPL1	LD phospholipase	438906	440258	-1	Phospholipase	Phospholipase; contains lipase specific GXSXG motif; maintains lipid droplet (LD) morphology; induced by transcription factor Rpn4p; protein abundance increases in response to DNA replication stress
S000005586	YOR060C	SLD7	Synthetic Lethality with Dpb11-24	440390	441163	-1	Protein with a role in chromosomal DNA replication	Protein with a role in chromosomal DNA replication; interacts with Sld3p and reduces its affinity for Cdc45p; deletion mutant has aberrant mitochondria; ortholog of human MTBP, which is a DNA replication origin firing factor
S000005587	YOR061W	CKA2	Casein Kinase Alpha' subunit	441534	442553	1	Alpha' catalytic subunit of casein kinase 2 (CK2)	Alpha' catalytic subunit of casein kinase 2 (CK2); CK2 is a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases; protein abundance increases in response to DNA replication stress; regulates Fkh1p-mediated donor preference during mating-type switching
S000005588	YOR062C	""	""	442725	443531	-1	Protein of unknown function	Protein of unknown function; similar to Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; YOR062C has a paralog, YKR075C, that arose from the whole genome duplication
S000005590	YOR064C	YNG1	Yeast iNG1 homolog	446079	446738	-1	Subunit of the NuA3 histone acetyltransferase complex	Subunit of the NuA3 histone acetyltransferase complex; this complex acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3; shares significant sequence identity with the human candidate tumor suppressor p33-ING1 in C-terminal region
S000005591	YOR065W	CYT1	CYTochrome c1	447439	448368	1	Cytochrome c1	Cytochrome c1; component of the mitochondrial respiratory chain; expression is regulated by the heme-activated, glucose-repressed Hap2p/3p/4p/5p CCAAT-binding complex
S000005592	YOR066W	MSA1	Mbf and Sbf Associated	449436	451325	1	Activator of G1-specific transcription factors MBF and SBF	Activator of G1-specific transcription factors MBF and SBF; involved in regulation of the timing of G1-specific gene transcription and cell cycle initiation; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; MSA1 has a paralog, MSA2, that arose from the whole genome duplication
S000005593	YOR067C	ALG8	Asparagine-Linked Glycosylation	451729	453462	-1	Glucosyl transferase	Glucosyl transferase; involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p; human homolog ALG8 can complement yeast null mutant
S000005594	YOR068C	VAM10	VAcuolar Morphogenesis	453869	454213	-1	Protein involved in vacuole morphogenesis	Protein involved in vacuole morphogenesis; acts at an early step of homotypic vacuole fusion that is required for vacuole tethering
S000005595	YOR069W	VPS5	Vacuolar Protein Sorting	453768	455795	1	Nexin-1 homolog	Nexin-1 homolog; required for localizing membrane proteins from a prevacuolar/late endosomal compartment back to late Golgi; structural component of retromer membrane coat complex; forms a retromer subcomplex with Vps17p; required for recruiting the retromer complex to the endosome membranes; VPS5 has a paralog, YKR078W, that arose from the whole genome duplication
S000005596	YOR070C	GYP1	Gtpase-activating protein for YPt1p	455907	457820	-1	Cis-golgi GTPase-activating protein (GAP) for yeast Rabs	Cis-golgi GTPase-activating protein (GAP) for yeast Rabs; the Rab family members are Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion; interacts with autophagosome component Atg8p
S000005597	YOR071C	NRT1	Nicotinamide Riboside Transporter	459480	461276	-1	High-affinity nicotinamide riboside transporter	High-affinity nicotinamide riboside transporter; also transports thiamine with low affinity; major transporter for 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside (acadesine) uptake; shares sequence similarity with Thi7p and Thi72p; proposed to be involved in 5-fluorocytosine sensitivity
S000005598	YOR072W	""	""	461502	461816	1	Putative protein of unknown function	Putative protein of unknown function; conserved across S. cerevisiae strains; partially overlaps the dubious gene YOR072W-A; diploid deletion strains are methotrexate, paraquat and wortmannin sensitive
S000005599	YOR073W	SGO1	ShuGOshin (Japanese for "guardian spirit")	464771	466543	1	Component of the spindle checkpoint	Component of the spindle checkpoint; involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability; recruits condensin to the pericentric region of chromosomes during meiosis; dissociates from pericentromeres when sister kinetochores are under tension
S000005600	YOR074C	CDC21	Cell Division Cycle	466675	467589	-1	Thymidylate synthase	Thymidylate synthase; required for de novo biosynthesis of pyrimidine deoxyribonucleotides; expression is induced at G1/S; human homolog TYMSOS can complement yeast cdc21 temperature-sensitive mutant at restrictive temperature
S000005601	YOR075W	UFE1	Unknown Function Essential	468212	469252	1	t-SNARE protein required for retrograde vesicular traffic	t-SNARE protein required for retrograde vesicular traffic; involved in Sey1p-independent homotypic ER fusion; required for efficient nuclear fusion during mating; forms a complex with the SNAREs Sec22p, Sec20p and Use1p to mediate fusion of Golgi-derived vesicles at the ER
S000005602	YOR076C	SKI7	SuperKIller	469377	471620	-1	GTP-binding protein that couples the Ski complex and exosome	GTP-binding protein that couples the Ski complex and exosome; putative pseudo-translational GTPase involved in 3'-to-5' mRNA decay pathway; interacts with both the cytoplasmic exosome and the Ski complex; eRF3-like domain targets nonstop mRNA for degradation; null mutants have a superkiller phenotype; SKI7 has a paralog, HBS1, that arose from the whole genome duplication
S000005603	YOR077W	RTS2	""	471899	472597	1	Basic zinc-finger protein	Basic zinc-finger protein; similar to human and mouse Kin17 proteins which are chromatin-associated proteins involved in UV response and DNA replication
S000005604	YOR078W	BUD21	BUD site selection	472725	473369	1	Component of small ribosomal subunit (SSU) processome	Component of small ribosomal subunit (SSU) processome; interacts with the U3 snoRNA (Snr17a/b); involved in rRNA processing and maturation; required post-transcriptionally for efficient retrotransposition; absence results in decreased Ty1 Gag:GFP protein levels; originally isolated as bud-site selection mutant that displays a random budding pattern; ortholog of human NOL7
S000005605	YOR079C	ATX2	AnTioXidant	473475	474416	-1	Golgi membrane protein involved in manganese homeostasis	Golgi membrane protein involved in manganese homeostasis; overproduction suppresses the sod1 (copper, zinc superoxide dismutase) null mutation
S000005606	YOR080W	DIA2	Digs Into Agar	474594	476792	1	Origin-binding F-box protein	Origin-binding F-box protein; forms SCF ubiquitin ligase complex with Skp1p and Cdc53p; functions in ubiquitination of silent chromatin structural protein Sir4p; required to target Cdc6p for destruction during G1 phase; required for deactivation of Rad53 checkpoint kinase, completion of DNA replication during recovery from DNA damage, assembly of RSC complex, RSC-mediated transcription regulation, and nucleosome positioning; involved in invasive and pseudohyphal growth
S000005607	YOR081C	TGL5	TriacylGlycerol Lipase	476939	479188	-1	Bifunctional triacylglycerol lipase and LPA acyltransferase	Bifunctional triacylglycerol lipase and LPA acyltransferase; lipid particle-localized triacylglycerol (TAG) lipase involved in triacylglycerol mobilization; catalyzes acylation of lysophosphatidic acid (LPA); potential Cdc28p substrate; TGL5 has a paralog, TGL4, that arose from the whole genome duplication
S000005608	YOR082C	""	""	479296	479637	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR083W
S000005609	YOR083W	WHI5	WHIskey	479533	480420	1	Repressor of G1 transcription	Repressor of G1 transcription; binds to SCB binding factor (SBF) at SCB target promoters in early G1; WHI5 expression is repressed in M/early G phase; phosphorylation of Whi5p by G1 cyclin (Cln)/Cdc28p relieves repression and promoter binding by Whi5p, drives nuclear export, and contributes to both the determination of critical cell size at START and cell fate; dephosphorylated and re-imported into the nucleus post-START during the starvation response, reversing commitment
S000005610	YOR084W	LPX1	Lipase of PeroXisomes	480587	481750	1	Peroxisomal matrix-localized lipase	Peroxisomal matrix-localized lipase; required for normal peroxisome morphology; contains a peroxisomal targeting signal type 1 (PTS1) and a lipase motif; peroxisomal import requires the PTS1 receptor, Pex5p and self-interaction; transcriptionally activated by Yrm1p along with genes involved in multidrug resistance; oleic acid inducible
S000005611	YOR085W	OST3	OligoSaccharylTransferase	482033	483085	1	Gamma subunit of the oligosaccharyltransferase complex of the ER lumen	Gamma subunit of the oligosaccharyltransferase complex of the ER lumen; complex catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins including modification of Pmt2p at N131 and N403
S000005612	YOR086C	TCB1	Three Calcium and lipid Binding domains (TriCalBins)	483219	486779	-1	Lipid-binding ER tricalbin involved in ER-plasma membrane tethering	Lipid-binding ER tricalbin involved in ER-plasma membrane tethering; one of at least 7 proteins (Ice2p, Ist2p, Scs2/Scs22p, Tcb1-Tcb3p) that affect cER tethering and contact with the plasma membrane (PM); regulates PI4P levels by controlling access of the Sac1p phosphatase to its substrate PI4P in the PM; regulates PM phospholipid homeostasis; contains 3 calcium and lipid binding domains; localizes to the cortical ER and to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact
S000005613	YOR087W	YVC1	Yeast Vacuolar Conductance	487707	489734	1	Vacuolar cation channel	Vacuolar cation channel; mediates release of Ca(2+) from the vacuole in response to hyperosmotic shock
S000005614	YOR088W	""	""	488286	489734	1	Merged open reading frame	Merged open reading frame; does not encode a discrete protein; YOR088W was originally annotated as an independent ORF, but as a result of a sequence change, it was merged with an adjacent ORF into a single reading frame, designated YOR087W
S000005615	YOR089C	VPS21	Vacuolar Protein Sorting	490196	490828	-1	Endosomal Rab family GTPase	Endosomal Rab family GTPase; required for endocytic transport and sorting of vacuolar hydrolases; required for endosomal localization of the CORVET complex; required with YPT52 for MVB biogenesis and sorting; involved in autophagy and ionic stress tolerance; geranylgeranylation required for membrane association; protein abundance increases in response to DNA replication stress; mammalian Rab5 homolog; VPS21 has a paralog, YPT53, that arose from the whole genome duplication
S000005616	YOR090C	PTC5	Phosphatase Two C	491123	492841	-1	Mitochondrial type 2C protein phosphatase (PP2C)	Mitochondrial type 2C protein phosphatase (PP2C); involved in regulation of pyruvate dehydrogenase activity by dephosphorylating the serine 133 of the Pda1p subunit; localizes to the intermembrane space and is imported via the presequence pathway and processed by the inner membrane protease (Imp1p-Imp2p); acts in concert with kinases Pkp1p and Pkp2p and phosphatase Ptc6p
S000005617	YOR091W	TMA46	Translation Machinery Associated	493433	494470	1	Protein of unknown function that associates with translating ribosomes	Protein of unknown function that associates with translating ribosomes; interacts with GTPase Rbg1p
S000005618	YOR092W	ECM3	ExtraCellular Mutant	495127	496968	1	Non-essential protein of unknown function	Non-essential protein of unknown function; involved in signal transduction and the genotoxic response; induced rapidly in response to treatment with 8-methoxypsoralen and UVA irradiation; relocalizes from ER to cytoplasm upon DNA replication stress; ECM3 has a paralog, YNL095C, that arose from the whole genome duplication
S000005619	YOR093C	CMR2	Changed Mutation Rate	497506	502452	-1	Protein involved in regulation of diacylglycerol lipids levels	Protein involved in regulation of diacylglycerol lipids levels; deletion causes sensitivity to unfolded protein response-inducing agents
S000005620	YOR094W	ARF3	ADP-Ribosylation Factor	502795	503346	1	Glucose-repressible ADP-ribosylation factor	Glucose-repressible ADP-ribosylation factor; GTPase of Ras superfamily involved in regulating cell polarity and invasive growth; localizes to dynamic spots at plasma membrane and modulates PtdIns(4,5)P2 levels to facilitate endocytosis; required for localization of endocytic protein Lsb5p to correct cortical site in cells; also has mRNA binding activity; homolog of mammalian Arf6
S000005621	YOR095C	RKI1	Ribose-5-phosphate Ketol-Isomerase	503552	504328	-1	Ribose-5-phosphate ketol-isomerase	Ribose-5-phosphate ketol-isomerase; catalyzes the interconversion of ribose 5-phosphate and ribulose 5-phosphate in the pentose phosphate pathway; participates in pyridoxine biosynthesis
S000005622	YOR096W	RPS7A	Ribosomal Protein of the Small subunit	505794	506767	1	Protein component of the small (40S) ribosomal subunit	Protein component of the small (40S) ribosomal subunit; interacts with Kti11p; deletion causes hypersensitivity to zymocin; homologous to mammalian ribosomal protein S7, no bacterial homolog; RPS7A has a paralog, RPS7B, that arose from the whole genome duplication
S000005623	YOR097C	""	""	506978	507505	-1	Putative protein of unknown function	Putative protein of unknown function; identified as interacting with Hsp82p in a high-throughput two-hybrid screen; YOR097C is not an essential gene
S000005624	YOR098C	NUP1	NUclear Pore	507948	511178	-1	FG-nucleoporin component of central core of the nuclear pore complex	FG-nucleoporin component of central core of the nuclear pore complex; contributes directly to nucleocytoplasmic transport and maintenance of thenuclear pore complex (NPC) permeability barrier; possible karyopherin release factor that accelerates release of karyopherin-cargo complexes after transport across NPC; both NUP1 and NUP60 are homologous to human NUP153
S000005625	YOR099W	KTR1	Kre Two Related	511825	513006	1	Alpha-1,2-mannosyltransferase	Alpha-1,2-mannosyltransferase; involved in O- and N-linked protein glycosylation; type II membrane protein; member of the KRE2/MNT1 mannosyltransferase family; relocalizes from vacuole to cytoplasm upon DNA replication stress
S000005626	YOR100C	CRC1	CaRnitine Carrier	513295	514278	-1	Mitochondrial inner membrane carnitine transporter	Mitochondrial inner membrane carnitine transporter; required for carnitine-dependent transport of acetyl-CoA from peroxisomes to mitochondria during fatty acid beta-oxidation; human homolog SLC25A20 complements yeast null mutant
S000005627	YOR101W	RAS1	homologous to RAS proto-oncogene	515244	516173	1	GTPase involved in G-protein signaling in adenylate cyclase activation	GTPase involved in G-protein signaling in adenylate cyclase activation; plays a role in regulation of cell proliferation; localized to the plasma membrane; activated by increased levels of glycolysis intermediate fructose-1,6-bisphosphate; homolog of mammalian RAS proto-oncogenes; relative distribution to the nucleus increases upon DNA replication stress; RAS1 has a paralog, RAS2, that arose from the whole genome duplication
S000005629	YOR103C	OST2	OligoSaccharylTransferase	516449	516841	-1	Non-catalytic epsilon subunit of the oligosaccharyltransferase (OST) c	Non-catalytic epsilon subunit of the oligosaccharyltransferase (OST) complex; the OST complex catalyzes asparagine-linked glycosylation of newly synthesized proteins in the ER; forms a pocket with Wbp1p involved in binding terminal glucose units of the donor glycan; located in the ER lumen
S000005630	YOR104W	PIN2	Psi+ INducibility	517642	518490	1	Exomer-dependent cargo protein	Exomer-dependent cargo protein; induces appearance of [PIN+] prion when overproduced; prion-like domain serves as a retention signal in the trans-Golgi network; predicted to be palmitoylated
S000005631	YOR105W	""	""	518195	518521	1	Protein of unknown function	Protein of unknown function; expressed at both mRNA and protein levels
S000005632	YOR106W	VAM3	VAcuolar Morphogenesis	519121	519972	1	Syntaxin-like vacuolar t-SNARE	Syntaxin-like vacuolar t-SNARE; functions with Vam7p in vacuolar protein trafficking; mediates docking/fusion of late transport intermediates with the vacuole; has an acidic di-leucine sorting signal and C-terminal transmembrane region
S000005633	YOR107W	RGS2	Regulator of heterotrimeric G protein Signaling	521353	522282	1	Negative regulator of glucose-induced cAMP signaling	Negative regulator of glucose-induced cAMP signaling; directly activates the GTPase activity of the heterotrimeric G protein alpha subunit Gpa2p
S000005634	YOR108W	LEU9	LEUcine biosynthesis	523027	524841	1	Alpha-isopropylmalate synthase II (2-isopropylmalate synthase)	Alpha-isopropylmalate synthase II (2-isopropylmalate synthase); catalyzes the first step in the leucine biosynthesis pathway; the minor isozyme, responsible for the residual alpha-IPMS activity detected in a leu4 null mutant; LEU9 has a paralog, LEU4, that arose from the whole genome duplication
S000005635	YOR109W	INP53	INositol polyphosphate 5-Phosphatase	525278	528601	1	Polyphosphatidylinositol phosphatase	Polyphosphatidylinositol phosphatase; dephosphorylates multiple phosphatidylinositol phosphates; involved in trans Golgi network-to-early endosome pathway; hyperosmotic stress causes translocation to actin patches; contains Sac1 and 5-ptase domains; INP53 has a paralog, INP52, that arose from the whole genome duplication
S000005636	YOR110W	TFC7	""	528941	530248	1	RNA pol III transcription initiation factor complex (TFIIIC) subunit	RNA pol III transcription initiation factor complex (TFIIIC) subunit; part of the TauA globular domain of TFIIIC that binds DNA at the BoxA promoter sites of tRNA and similar genes; TFC7 has a paralog, YNL108C, that arose from the whole genome duplication
S000005637	YOR111W	""	""	530429	531127	1	Putative protein of unknown function	Putative protein of unknown function
S000005638	YOR112W	CEX1	Cytoplasmic EXport protein	531508	533793	1	Component of COPI-mediated trafficking and tRNA nuclear export	Component of COPI-mediated trafficking and tRNA nuclear export; enables Rna1p to access and activate Gsp1p-GTP bound to the export receptor tRNA complex during aminoacylation-dependent tRNA export; copurifies with tRNA export receptors; interacts with COPI coat proteins and regulates Golgi-to-ER trafficking; membrane fraction associated; mutations in members of the homologous human SCY1-like (SCYL) family of pseudokinases are linked to peripheral neuropathy, cerebellar atrophy, ataxia and ALS
S000005639	YOR113W	AZF1	Asparagine-rich Zinc-Finger	534075	536819	1	Zinc-finger transcription factor	Zinc-finger transcription factor; involved in the diauxic shift; activates transcription of genes involved in carbon metabolism and energy production on glucose, while it activates transcription of genes involved in cell wall organization and biogenesis on glycerol-lactate; may also function as a co-repressor; capable of forming the prion [AZF1+]; relocalizes to the cytosol in response to hypoxia; contains polyglutamine and polyasparagine domains
S000005640	YOR114W	DPI34	Delta-Psi dependent mitochondrial Import protein of ~34 kDa	537570	538454	1	Putative mitochondrial protein of unknown function	Putative mitochondrial protein of unknown function; null mutant is viable
S000005641	YOR115C	TRS33	TRapp Subunit	538659	539465	-1	Core component of TRAPP complexes I, II and IV	Core component of TRAPP complexes I, II and IV; transport protein particle (TRAPP) complexes are related multimeric guanine nucleotide-exchange factor for the GTPase Ypt1p, regulating ER-Golgi traffic (TRAPPI), intra-Golgi traffic (TRAPPII), endosome-Golgi traffic (TRAPPII and III) and autophagy (TRAPPIII, and IV); proposed subunit of a novel complex, TRAPPIV, that may function redundantly with TRAPPIII as a GEF that activates Ypt1 during autophagy
S000005642	YOR116C	RPO31	RNA POlymerase	539763	544145	-1	RNA polymerase III largest subunit C160	RNA polymerase III largest subunit C160; part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
S000005643	YOR117W	RPT5	Regulatory Particle Triple-A protein, or Regulatory Particle Triphosphatase	545029	546333	1	ATPase of the 19S regulatory particle of the 26S proteasome	ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; recruited to the GAL1-10 promoter region upon induction of transcription; similar to human TBP1
S000005644	YOR118W	RTC5	Restriction of Telomere Capping	546857	548560	1	Protein of unknown function	Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; null mutation suppresses cdc13-1 temperature sensitivity
S000005645	YOR119C	RIO1	RIght Open reading frame	548792	550246	-1	Serine kinase that controls cell cycle progression	Serine kinase that controls cell cycle progression; represses rDNA transcription and promotes 20S pre-rRNA maturation; catalytic activity regulates its association with pre-40S particles where it prevents premature entry into translation; downregulates centromeric RNA levels, limits RNAPII accessibility, stimulates cenRNA degradation and promotes timely kinetochore assembly; autophosphorylates and phosphorylates Rpa43p in anaphase to remove Pol I from rDNA
S000005646	YOR120W	GCY1	Galactose-inducible Crystallin-like Yeast protein	551114	552052	1	Glycerol dehydrogenase	Glycerol dehydrogenase; involved in an alternative pathway for glycerol catabolism used under microaerobic conditions; also has mRNA binding activity; member of the aldo-keto reductase (AKR) family; human homolog AKR1B1 can complement yeast null mutant; protein abundance increases in response to DNA replication stress; GCY1 has a paralog, YPR1, that arose from the whole genome duplication
S000005647	YOR121C	""	""	551798	552103	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene GCY1/YOR120W
S000005648	YOR122C	PFY1	ProFilin of Yeast	552298	552887	-1	Profilin	Profilin; binds actin, phosphatidylinositol 4,5-bisphosphate, and polyproline regions; involved in cytoskeleton organization; required for normal timing of actin polymerization in response to thermal stress; protein abundance increases in response to DNA replication stress; highly conserved protein; human PFN1 (profilin 1) complements temperature sensitive pfy1 mutants, PFN1 mutations are a rare cause of ALS
S000005649	YOR123C	LEO1	LEft Open reading frame	553176	554570	-1	Component of the Paf1 complex	Component of the Paf1 complex; which associates with RNA polymerase II and is involved in histone methylation; plays a role in regulating Ty1 transposition; involved in transcription elongation as demonstrated by the G-less-based run-on (GLRO) assay
S000005650	YOR124C	UBP2	UBiquitin-specific Protease	554824	558642	-1	Ubiquitin-specific protease	Ubiquitin-specific protease; removes ubiquitin from ubiquitinated proteins; controls K63 homeostasis during oxidative stress; deubiquitinates Rsp5p and is required for MVB sorting of membrane proteins; can cleave polyubiquitin and has isopeptidase activity
S000005651	YOR125C	CAT5	CATabolite repression	559030	559731	-1	Protein required for ubiquinone (Coenzyme Q) biosynthesis	Protein required for ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes; required for gluconeogenic gene activation
S000005652	YOR126C	IAH1	Isoamyl Acetate-Hydrolyzing esterase	559961	560677	-1	Isoamyl acetate-hydrolyzing esterase	Isoamyl acetate-hydrolyzing esterase; required in balance with alcohol acetyltransferase to maintain optimal amounts of isoamyl acetate, which is particularly important in sake brewing
S000005653	YOR127W	RGA1	Rho GTPase Activating Protein	561170	564193	1	GTPase-activating protein for polarity-regulator Cdc42p (RhoGAP)	GTPase-activating protein for polarity-regulator Cdc42p (RhoGAP); required for proper bud site selection; transiently localizes to previous cell division sites (bud scars; cytokinesis remnants), interacting with Nis1p and Nba1p, to prevent Cdc42p repolarization; implicated in control of septin organization, pheromone response, and haploid invasive growth; relocalizes from the bud neck to cytoplasm upon DNA replication stress; contains N-terminal LIM domains and a C-terminal GAP domain
S000005654	YOR128C	ADE2	ADEnine requiring	564476	566191	-1	Phosphoribosylaminoimidazole carboxylase	Phosphoribosylaminoimidazole carboxylase; catalyzes a step in the 'de novo' purine nucleotide biosynthetic pathway; red pigment accumulates in mutant cells deprived of adenine
S000005655	YOR129C	AFI1	ArF3-Interacting protein	566877	569558	-1	Arf3p polarization-specific docking factor	Arf3p polarization-specific docking factor; required for the polarized distribution of the ADP-ribosylation factor, Arf3p; participates in polarity development and maintenance of a normal haploid budding pattern; interacts with Cnm7p
S000005656	YOR130C	ORT1	ORnithine Transporter	569929	570807	-1	Ornithine transporter of the mitochondrial inner membrane	Ornithine transporter of the mitochondrial inner membrane; exports ornithine from mitochondria as part of arginine biosynthesis; functionally complemented by human ortholog, SLC25A15, which is associated with hyperammonaemia-hyperornithinaemia-homocitrullinuria (HHH) syndrome, but HHH-associated variants fail to complement
S000005657	YOR131C	""	""	572182	572838	-1	Putative haloacid dehalogenase-like hydrolase	Putative haloacid dehalogenase-like hydrolase; non-essential gene; overexpression causes a cell cycle delay or arrest; protein abundance increases in response to DNA replication stress
S000005658	YOR132W	VPS17	Vacuolar Protein Sorting	573175	574830	1	Subunit of the membrane-associated retromer complex	Subunit of the membrane-associated retromer complex; essential for endosome-to-Golgi retrograde protein transport; peripheral membrane protein that assembles onto the membrane with Vps5p to promote vesicle formation; required for recruiting the retromer complex to the endosome membranes
S000005659	YOR133W	EFT1	Elongation Factor Two	575098	577626	1	Elongation factor 2 (eEF2, EF-2), also encoded by EFT2	Elongation factor 2 (eEF2, EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication
S000005660	YOR134W	BAG7	""	578564	579793	1	Rho GTPase activating protein (RhoGAP)	Rho GTPase activating protein (RhoGAP); stimulates the intrinsic GTPase activity of Rho1p, which plays a bud growth by regulating actin cytoskeleton organization and cell wall biosynthesis, resulting in the downregulation of Rho1p; structurally and functionally related to Sac7p; BAG7 has a paralog, SAC7, that arose from the whole genome duplication
S000005661	YOR135C	IRC14	""	580159	580500	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YOR136W; null mutant displays increased levels of spontaneous Rad52 foci
S000005662	YOR136W	IDH2	Isocitrate DeHydrogenase	580250	581359	1	Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase	Subunit of mitochondrial NAD(+)-dependent isocitrate dehydrogenase; complex catalyzes oxidation of isocitrate to alpha-ketoglutarate in TCA cycle; targeted to vacuole via AP-3 pathway
S000005663	YOR137C	SIA1	Suppressor of eIF5A	581813	583681	-1	Protein of unassigned function	Protein of unassigned function; involved in activation of the Pma1p plasma membrane H+-ATPase by glucose; contains peptide signal for membrane localization
S000005664	YOR138C	RUP1	Rsp5-Ubp2 interacting Protein	584309	586324	-1	Protein that regulates ubiquitination of Rsp5p	Protein that regulates ubiquitination of Rsp5p; has a WW domain consensus motif of PPPSY (residues 131-135) that mediates binding of Rsp5p to Ubp2p; contains an UBA domain; relative distribution to the nucleus increases upon DNA replication stress
S000005665	YOR139C	""	""	586950	587342	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SFL1/YOR140W
S000005666	YOR140W	SFL1	Suppressor gene for FLocculation	586981	589281	1	Transcriptional repressor and activator	Transcriptional repressor and activator; involved in repression of flocculation-related genes, and activation of stress responsive genes; has direct role in INO1 transcriptional memory; negatively regulated by cAMP-dependent protein kinase A subunit Tpk2p; premature stop codon (C1430T, Q477-stop) in SK1 background is linked to the aggressively invasive phenotype of SK1 relative to BY4741 (S288C)
S000005667	YOR141C	ARP8	Actin-Related Protein	589942	592587	-1	Nuclear actin-related protein involved in chromatin remodeling	Nuclear actin-related protein involved in chromatin remodeling; component of chromatin-remodeling enzyme complexes; has mRNA binding activity
S000005668	YOR142W	LSC1	Ligase of Succinyl-CoA	593057	594046	1	Alpha subunit of succinyl-CoA ligase	Alpha subunit of succinyl-CoA ligase; succinyl-CoA ligase is a mitochondrial enzyme of the TCA cycle that catalyzes the nucleotide-dependent conversion of succinyl-CoA to succinate; phosphorylated
S000005670	YOR144C	ELG1	Enhanced Level of Genomic instability	602717	605092	-1	Subunit of an alternative replication factor C complex	Subunit of an alternative replication factor C complex; important for DNA replication and genome integrity; suppresses spontaneous DNA damage; involved in homologous recombination-mediated repair and telomere homeostasis; required for PCNA (Pol30p) unloading during DNA replication
S000005671	YOR145C	PNO1	Partner of NOb1	605347	606171	-1	Essential nucleolar protein required for pre-18S rRNA processing	Essential nucleolar protein required for pre-18S rRNA processing; interacts with Dim1p, an 18S rRNA dimethyltransferase, and also with Nob1p, which is involved in proteasome biogenesis; contains a KH domain
S000005673	YOR147W	MDM32	Mitochondrial Distribution and Morphology	606607	608475	1	Mitochondrial inner membrane protein with similarity to Mdm31p	Mitochondrial inner membrane protein with similarity to Mdm31p; required for normal mitochondrial morphology and inheritance; interacts genetically with MMM1, MDM10, MDM12, and MDM34; variation between SK1 and S288C at residues 182 and 262 impacts invasive growth and mitochondrial network structure
S000005674	YOR148C	SPP2	Suppressor of PrP	608640	609197	-1	Essential protein that promotes the first step of splicing	Essential protein that promotes the first step of splicing; required for the final stages of spliceosome maturation and activation; interacts with Prp2p, which may release Spp2p from the spliceosome following the first cleavage reaction; stimulates Prp2p ATPase activity
S000005675	YOR149C	SMP3	Stable Maintenance of pSRI	609838	611388	-1	Alpha 1,2-mannosyltransferase	Alpha 1,2-mannosyltransferase; involved in glycosyl phosphatidyl inositol (GPI) biosynthesis; required for addition of the fourth, side branching mannose to the GPI core structure
S000005676	YOR150W	MRPL23	Mitochondrial Ribosomal Protein, Large subunit	611999	612490	1	Mitochondrial ribosomal protein of the large subunit	Mitochondrial ribosomal protein of the large subunit; localizes to vacuole in response to H2O2
S000005677	YOR151C	RPB2	RNA Polymerase B	612997	616671	-1	RNA polymerase II second largest subunit B150	RNA polymerase II second largest subunit B150; part of central core; similar to bacterial beta subunit
S000005678	YOR152C	ATG40	AuTophaGy related	617518	618288	-1	Autophagy receptor with a role in endoplasmic reticulum degradation	Autophagy receptor with a role in endoplasmic reticulum degradation; involved specifically in autophagy of cortical and cytoplasmic ER in response to nitrogen starvation or rapamycin treatment; acts with COPII subunit Sfb3p; localizes to the cortical and cytoplasmic ER; similar to human FAM134B, which is also involved in ER autophagy and is associated with sensory neuropathy
S000005679	YOR153W	PDR5	Pleiotropic Drug Resistance	619840	624375	1	Plasma membrane ATP-binding cassette (ABC) transporter	Plasma membrane ATP-binding cassette (ABC) transporter; multidrug transporter actively regulated by Pdr1p; also involved in steroid transport, cation resistance, and cellular detoxification during exponential growth; PDR5 has a paralog, PDR15, that arose from the whole genome duplication
S000005680	YOR154W	SLP1	SUN-Like Protein	624729	626492	1	Glycosylated integral ER membrane protein of unknown function	Glycosylated integral ER membrane protein of unknown function; forms an ER-membrane associated protein complex with Emp65p; member of the SUN-like family of proteins; genetic interactions suggest a role in folding of ER membrane proteins; required for nuclear envelope localization of Mps3p
S000005681	YOR155C	ISN1	IMP-Specific 5'-Nucleotidase	626628	627980	-1	Inosine 5'-monophosphate (IMP)-specific 5'-nucleotidase	Inosine 5'-monophosphate (IMP)-specific 5'-nucleotidase; catalyzes the breakdown of IMP to inosine; responsible for production of nicotinamide riboside and nicotinic acid riboside; expression positively regulated by nicotinic acid and glucose availability; does not show similarity to known 5'-nucleotidases from other organisms
S000005682	YOR156C	NFI1	Neck Filament Interacting	628360	630540	-1	SUMO E3 ligase	SUMO E3 ligase; catalyzes sumoylation of Yku70p/80p and Sir4p promoting telomere anchoring to the nuclear envelope and regulating telomerase activity; DNA-bound form catalyzes a DNA-damaged triggered sumoylation wave resulting in multisite modification of several DNA repair proteins, enhancing interactions between these proteins and accelerating repair; sumoylates Cse4p, a prerequisite for STUbL-mediated Ub-dependent degradation; role in telomere length maintenance
S000005683	YOR157C	PUP1	PUtative Proteasome subunit	630966	631751	-1	Beta 2 subunit of the 20S proteasome	Beta 2 subunit of the 20S proteasome; endopeptidase with trypsin-like activity that cleaves after basic residues; synthesized as a proprotein before being proteolytically processed for assembly into 20S particle; human homolog is subunit Z
S000005684	YOR158W	PET123	PETite colonies	632164	633120	1	Mitochondrial ribosomal protein of the small subunit	Mitochondrial ribosomal protein of the small subunit; PET123 exhibits genetic interactions with PET122, which encodes a COX3 mRNA-specific translational activator
S000005685	YOR159C	SME1	Sm protein E	633282	633566	-1	Core Sm protein Sm E	Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E
S000005686	YOR160W	MTR10	Mrna TRansport defective	633839	636757	1	Nuclear import receptor	Nuclear import receptor; mediates the nuclear localization of proteins involved in mRNA-nucleus export; promotes dissociation of mRNAs from the nucleus-cytoplasm mRNA shuttling protein Npl3p; required for retrograde import of mature tRNAs; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress
S000005687	YOR161C	PNS1	pH Nine Sensitive	636939	638558	-1	Protein of unknown function	Protein of unknown function; has similarity to Torpedo californica tCTL1p, which is postulated to be a choline transporter, neither null mutation nor overexpression affects choline transport
S000005688	YOR162C	YRR1	Yeast Reveromycin-A Resistant	639560	641992	-1	Zn2-Cys6 zinc-finger transcription factor	Zn2-Cys6 zinc-finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrm1p, acting on an overlapping set of target genes; YRR1 has a paralog, PDR8, that arose from the whole genome duplication
S000005689	YOR163W	DDP1	Diadenosine and Diphosphoinositol Polyphosphate phosphohydrolase	642741	643307	1	Diadenosine and diphosphoinositol polyphosphate phosphatase	Diadenosine and diphosphoinositol polyphosphate phosphatase; hydrolyzes diphosphorylated inositol polyphosphates and diadenosine polyphosphates; high specificity for diadenosine hexa- and pentaphosphates; contains endopolyphosphatase activity with a high affinity for polyphosphates, an activity also observed for its human DIPP homologs; possesses mRNA decapping activity; nudix hydrolase family member; protein abundance increases in response to DNA replication stress
S000005690	YOR164C	GET4	Guided Entry Tail-anchored proteins	643394	644332	-1	Protein involved in inserting tail-anchored proteins into ER membranes	Protein involved in inserting tail-anchored proteins into ER membranes; forms a complex with Mdy2p; highly conserved across species and homologous to human gene C7orf20
S000005691	YOR165W	SEY1	Synthetic Enhancement of YOP1	644566	646896	1	Dynamin-like GTPase that mediates homotypic ER fusion	Dynamin-like GTPase that mediates homotypic ER fusion; has a role in ER morphology; interacts physically and genetically with Yop1p and Rtn1p; functional ortholog of the human atlastin ATL1, defects in which cause a form of the human disease hereditary spastic paraplegia; homolog of Arabidopsis RHD3
S000005692	YOR166C	SWT1	Synthetically lethal With Trex	647126	648502	-1	RNA endoribonuclease involved in perinuclear mRNP quality control	RNA endoribonuclease involved in perinuclear mRNP quality control; involved in perinuclear mRNP quality control via the turnover of aberrant, unprocessed pre-mRNAs; interacts with subunits of THO/TREX, TREX-2, and RNA polymerase II; contains a PIN (PilT N terminus) domain
S000005693	YOR167C	RPS28A	Ribosomal Protein of the Small subunit	648804	649007	-1	Protein component of the small (40S) ribosomal subunit	Protein component of the small (40S) ribosomal subunit; has an extraribosomal function in regulation of RPS28B, in which Rps28Ap binds to a decapping complex via Edc3p, which then binds to RPS28B mRNA leading to its decapping and degradation; homologous to mammalian ribosomal protein S28, no bacterial homolog; RPS28A has a paralog, RPS28B, that arose from the whole genome duplication
S000005694	YOR168W	GLN4	GLutamiNe metabolism	649303	651732	1	Glutamine tRNA synthetase	Glutamine tRNA synthetase; monomeric class I tRNA synthetase that catalyzes the specific glutaminylation of tRNA(Gln); N-terminal domain proposed to be involved in enzyme-tRNA interactions
S000005696	YOR170W	""	""	651858	652163	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene LCB4
S000005697	YOR171C	LCB4	Long-Chain Base	652010	653884	-1	Sphingoid long-chain base kinase	Sphingoid long-chain base kinase; responsible for synthesis of long-chain base phosphates, which function as signaling molecules, regulates synthesis of ceramide from exogenous long-chain bases, localizes to the Golgi and late endosomes; LCB4 has a paralog, LCB5, that arose from the whole genome duplication
S000005698	YOR172W	YRM1	Yeast Reveromycin resistance Modulator	654210	656570	1	Zinc finger transcription factor involved in multidrug resistance	Zinc finger transcription factor involved in multidrug resistance; Zn(2)-Cys(6) zinc finger transcription factor; activates genes involved in multidrug resistance; paralog of Yrr1p, acting on an overlapping set of target genes
S000005699	YOR173W	DCS2	DeCapping Scavenger	657264	658325	1	m(7)GpppX pyrophosphatase regulator	m(7)GpppX pyrophosphatase regulator; non-essential, stress induced regulatory protein; modulates m7G-oligoribonucleotide metabolism; inhibits Dcs1p; regulated by Msn2p, Msn4p, and the Ras-cAMP-cAPK signaling pathway; mutant has increased aneuploidy tolerance; DCS2 has a paralog, DCS1, that arose from the whole genome duplication
S000005700	YOR174W	MED4	MEDiator complex	658747	659601	1	Subunit of the RNA polymerase II mediator complex	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
S000005701	YOR175C	ALE1	Acyltransferase for Lyso-phosphatidylEthanolamine	659815	661674	-1	Broad-specificity lysophospholipid acyltransferase	Broad-specificity lysophospholipid acyltransferase; part of MBOAT family of membrane-bound O-acyltransferases; key component of Lands cycle; may have role in fatty acid exchange at sn-2 position of mature glycerophospholipids
S000005702	YOR176W	HEM15	HEMe biosynthesis	662401	663582	1	Ferrochelatase	Ferrochelatase; a mitochondrial inner membrane protein, catalyzes insertion of ferrous iron into protoporphyrin IX, the eighth and final step in the heme biosynthetic pathway; human homolog FECH can complement yeast mutant and allow growth of haploid null after sporulation of a heterozygous diploid
S000005703	YOR177C	MPC54	Meiotic Plaque Component	665785	667179	-1	Component of the meiotic outer plaque	Component of the meiotic outer plaque; a membrane-organizing center which is assembled on the cytoplasmic face of the spindle pole body during meiosis II and triggers the formation of the prospore membrane; potential Cdc28p substrate
S000005704	YOR178C	GAC1	Glycogen ACcumulation	667860	670241	-1	Regulatory subunit for Glc7p type-1 protein phosphatase (PP1)	Regulatory subunit for Glc7p type-1 protein phosphatase (PP1); tethers Glc7p to Gsy2p glycogen synthase, binds Hsf1p heat shock transcription factor, required for induction of some HSF-regulated genes under heat shock; GAC1 has a paralog, PIG1, that arose from the whole genome duplication
S000005705	YOR179C	SYC1	Similar to Ysh1 C-terminal	671845	672411	-1	Subunit of the APT subcomplex of cleavage and polyadenylation factor	Subunit of the APT subcomplex of cleavage and polyadenylation factor; may have a role in 3' end formation of both polyadenylated and non-polyadenylated RNAs; SYC1 has a paralog, YSH1, that arose from the whole genome duplication
S000005706	YOR180C	DCI1	delta(3,5)-delta(2,4)-Dienoyl-CoA Isomerase	674352	675167	-1	Peroxisomal protein	Peroxisomal protein; identification as a delta(3,5)-delta(2,4)-dienoyl-CoA isomerase involved in fatty acid metabolism is disputed; DCI1 has a paralog, ECI1, that arose from the whole genome duplication
S000005707	YOR181W	LAS17	""	675939	677840	1	Actin assembly factor	Actin assembly factor; C-terminal WCA domain activates Arp2/3 complex-mediated nucleation of branched actin filaments, polyproline domain nucleates actin filaments independent of Arp2/3; mutants are defective in endocytosis, bud site selection, cytokinesis; human homolog WAS (Wiskott-Aldrich Syndrome) implicated in severe immunodeficiency; human WAS complements yeast null mutant, but only in presence of WIPF1, which mediates localization of WAS to cortical patches
S000005708	YOR182C	RPS30B	Ribosomal Protein of the Small subunit	678191	678793	-1	Protein component of the small (40S) ribosomal subunit	Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S30, no bacterial homolog; RPS30B has a paralog, RPS30A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
S000005709	YOR183W	FYV12	Function required for Yeast Viability	678872	679261	1	Protein of unknown function	Protein of unknown function; required for survival upon exposure to K1 killer toxin
S000005710	YOR184W	SER1	SERine requiring	679357	680544	1	3-phosphoserine aminotransferase	3-phosphoserine aminotransferase; catalyzes the formation of phosphoserine from 3-phosphohydroxypyruvate, required for serine and glycine biosynthesis; regulated by the general control of amino acid biosynthesis mediated by Gcn4p; protein abundance increases in response to DNA replication stress
S000005711	YOR185C	GSP2	Genetic Suppressor of Prp20-1	681444	682106	-1	GTP binding protein (mammalian Ranp homolog)	GTP binding protein (mammalian Ranp homolog); involved in the maintenance of nuclear organization, RNA processing and transport; interacts with Kap121p, Kap123p and Pdr6p (karyophilin betas); not required for viability; protein abundance increases in response to DNA replication stress; GSP2 has a paralog, GSP1, that arose from the whole genome duplication
S000005712	YOR186W	""	""	683111	683545	1	Putative protein of unknown function	Putative protein of unknown function; proper regulation of expression during heat stress is sphingolipid-dependent; mCherry fusion protein localizes to the vacuole; YOR186W has a paralog, YLR297W, that arose from the whole genome duplication
S000005713	YOR187W	TUF1	""	684030	685343	1	Mitochondrial translation elongation factor Tu (EF-Tu)	Mitochondrial translation elongation factor Tu (EF-Tu); involved in fundamental pathway of mtDNA homeostasis; comprises both GTPase and guanine nucleotide exchange factor activities, while these activities are found in separate proteins in S. pombe and humans; rare mutations in human mitochondrial elongation factor Tu (EFTu) associated with severe lactic acidosis, rapidly progressive fatal encephalopathy, severe infantile macrocystic leukodystrophy with micropolygyria
S000005714	YOR188W	MSB1	Multicopy Suppression of a Budding defect	685767	689180	1	Protein of unknown function	Protein of unknown function; may be involved in positive regulation of 1,3-beta-glucan synthesis and the Pkc1p-MAPK pathway; multicopy suppressor of temperature-sensitive mutations in CDC24 and CDC42, and of mutations in BEM4; potential Cdc28p substrate; relocalizes from bud neck to cytoplasm upon DNA replication stress
S000005715	YOR189W	IES4	Ino Eighty Subunit	689624	689974	1	Component of the INO80 chromatiin remodeling complex	Component of the INO80 chromatiin remodeling complex; target of the Mec1p/Tel1p DNA damage signaling pathway; proposed to link chromatin remodeling to replication checkpoint responses
S000005716	YOR190W	SPR1	SPorulation Regulated	690695	692032	1	Sporulation-specific exo-1,3-beta-glucanase	Sporulation-specific exo-1,3-beta-glucanase; contributes to ascospore thermoresistance; SPR1 has a paralog, EXG1, that arose from the whole genome duplication
S000005717	YOR191W	ULS1	Ubiquitin Ligase for SUMO conjugates	692475	697334	1	Swi2/Snf2-related translocase, SUMO-Targeted Ubiquitin Ligase (STUbL)	Swi2/Snf2-related translocase, SUMO-Targeted Ubiquitin Ligase (STUbL); required for maintenance of NHEJ inhibition at telomeres; functions at telomeres to translocate and ubiquitinylate poly-sumoylated Rap1p for proteosomal degradation; plays role in antagonizing silencing during mating-type switching; only known STUbL with a translocase activity; contains RING finger domain; relocalizes from nucleus to cytoplasm upon DNA replication stress
S000005718	YOR192C	THI72	THI7 homolog 2	698768	700567	-1	Transporter of thiamine or related compound	Transporter of thiamine or related compound; contributes to uptake of 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside (acadesine); shares sequence similarity with Thi7p
S000005719	YOR193W	PEX27	PEroXisome related	710446	711576	1	Peripheral peroxisomal membrane protein	Peripheral peroxisomal membrane protein; required for Vps1-dependent peroxisome fission but not Dnm1-dependent fission; functions to control peroxisome size and number; interacts with Vps1p and Pex25p; accumulates at peroxisomal membrane constrictions; PEX27 has a paralog, PEX25, that arose from the whole genome duplication
S000005720	YOR194C	TOA1	""	711683	712543	-1	TFIIA large subunit	TFIIA large subunit; involved in transcriptional activation, acts as antirepressor or as coactivator; required, along with Toa2p, for ribosomal protein gene transcription in vivo; homologous to largest and second largest subunits of human and Drosophila TFIIA
S000005721	YOR195W	SLK19	Synthetic Lethal Kar3p	712866	715331	1	Kinetochore-associated protein	Kinetochore-associated protein; required for chromosome segregation and kinetochore clustering; required for normal segregation of chromosomes in meiosis and mitosis; component of the FEAR regulatory network, which promotes Cdc14p release from the nucleolus during anaphase; potential Cdc28p substrate
S000005722	YOR196C	LIP5	LIPoic acid	715593	716837	-1	Protein involved in biosynthesis of the coenzyme lipoic acid	Protein involved in biosynthesis of the coenzyme lipoic acid; has similarity to E. coli lipoic acid synthase
S000005723	YOR197W	MCA1	MetaCAspase	717086	718384	1	Ca2+-dependent cysteine protease	Ca2+-dependent cysteine protease; may cleave specific substrates during the stress response; regulates apoptosis upon H2O2 treatment; required for clearance of insoluble protein aggregates during normal growth; implicated in cell cycle dynamics and lifespan extension; undergoes autocatalytic processing; similar to mammalian metacaspases, but exists as a monomer due to an extra pair of anti-parallel beta-strands that block potential dimerization
S000005724	YOR198C	BFR1	BreFeldin A Resistance	718653	720065	-1	Component of mRNP complexes associated with polyribosomes	Component of mRNP complexes associated with polyribosomes; involved in localization of mRNAs to P bodies; implicated in secretion and nuclear segregation; multicopy suppressor of BFA (Brefeldin A) sensitivity
S000005725	YOR199W	""	""	720181	720510	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000005726	YOR200W	""	""	720417	720815	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRM1/YOR201c
S000005727	YOR201C	MRM1	Mitochondrial rRNA Methyltransferase	720470	721708	-1	Ribose methyltransferase	Ribose methyltransferase; modifies a functionally critical, conserved nucleotide in mitochondrial 21S rRNA
S000005728	YOR202W	HIS3	HIStidine	721946	722608	1	Imidazoleglycerol-phosphate dehydratase	Imidazoleglycerol-phosphate dehydratase; catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p
S000005730	YOR204W	DED1	Defines Essential Domain	722911	724725	1	ATP-dependent DEAD-box RNA helicase with strand-annealing activity	ATP-dependent DEAD-box RNA helicase with strand-annealing activity; promotes eIF4F-dependent 48S translation preinitiation complex (PIC) assembly, stimulating recruitment of mRNAs with long, structured 5'-UTRs; cooperates with Dbp1p in PIC attachment and scanning; ATPase activity stimulated by mRNA cap-associated factor binding; directly binds eIF4G; role in spliceosomal complex disassembly; mutation in human homolog DBY associated with male infertility; human homolog DDX3X complements the null
S000005731	YOR205C	GEP3	GEnetic interactors of Prohibitins	725564	727234	-1	Protein required for mitochondrial ribosome small subunit biogenesis	Protein required for mitochondrial ribosome small subunit biogenesis; null mutant is defective in respiration and in maturation of 15S rRNA; protein is localized to the mitochondrial inner membrane; null mutant interacts synthetically with prohibitin (Phb1p)
S000005732	YOR206W	NOC2	NucleOlar Complex associated	727512	729644	1	Protein involved in ribosome biogenesis	Protein involved in ribosome biogenesis; forms a nucleolar complex with Mak21p that binds to 90S and 66S pre-ribosomes; forms a nuclear complex with Noc3p that binds to 66S pre-ribosomes; both complexes mediate intranuclear transport of ribosomal precursors; acts as part of a Mak21p-Noc2p-Rrp5p module that associates with nascent pre-rRNA during transcription and has a role in bigenesis of the large ribosomal subunit
S000005733	YOR207C	RET1	Reduced Efficiency of Termination	730008	733457	-1	Second-largest subunit of RNA polymerase III	Second-largest subunit of RNA polymerase III; RNA polymerase III is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
S000005734	YOR208W	PTP2	Protein Tyrosine Phosphatase	733925	736177	1	Phosphotyrosine-specific phosphatase	Phosphotyrosine-specific phosphatase; major role in osmolarity sensing through dephosphorylation of the Hog1p MAPK with a minor role by Ptp3p; inactivates and regulates Hog1p localization; major role in the cell wall integrity pathway through dephosphorylation of MAPK Slt2p with a minor role by Ptp3p; minor role with Msg5p in the pheromone adaptive response through dephosphorylation of MAPK Fus3p with major role by Ptp3p; co-regulates the calcium signaling pathway with Msg5p; nuclear localized
S000005735	YOR209C	NPT1	Nicotinate PhosphoribosylTransferase	736437	737726	-1	Nicotinate phosphoribosyltransferase	Nicotinate phosphoribosyltransferase; acts in the salvage pathway of NAD+ biosynthesis; required for silencing at rDNA and telomeres and has a role in silencing at mating-type loci; localized to the nucleus
S000005737	YOR211C	MGM1	Mitochondrial Genome Maintenance	738924	741569	-1	Mitochondrial GTPase, present in complex with Ugo1p and Fzo1p	Mitochondrial GTPase, present in complex with Ugo1p and Fzo1p; required for mitochondrial morphology, fusion, and genome maintenance; promotes membrane bending; plays a direct role in formation and maintenance of lamellar, but not of tubular, cristae; exists as long and short form with different distributions; ratio of long to short forms is regulated by Psd1p; homolog of human OPA1 involved in autosomal dominant optic atrophy
S000005738	YOR212W	STE4	STErile	742910	744181	1	G protein beta subunit	G protein beta subunit; forms a dimer with Ste18p to activate mating signaling pathway, forms heterotrimer with Gpa1p and Ste18p to dampen signaling; pheromone-induced phosphorylation plays critical role in chemotropism; may recruit Rho1p to polarized growth site during mating; contains WD40 repeats
S000005739	YOR213C	SAS5	Something About Silencing	744535	745281	-1	Subunit of the SAS complex (Sas2p, Sas4p, Sas5p)	Subunit of the SAS complex (Sas2p, Sas4p, Sas5p); acetylates free histones and nucleosomes and regulates transcriptional silencing; stimulates Sas2p HAT activity
S000005740	YOR214C	SPR2	SPorulation-Regulated	745593	746303	-1	Putative spore wall protein	Putative spore wall protein; expression increases during sporulation; not an essential gene; YOR214C has a paralog, SPO19, that arose from the whole genome duplication
S000005741	YOR215C	AIM41	Altered Inheritance of Mitochondria	746725	747282	-1	Protein of unknown function	Protein of unknown function; the authentic protein is detected in highly purified mitochondria in high-throughput studies; null mutant displays reduced frequency of mitochondrial genome loss
S000005742	YOR216C	RUD3	Relieves Uso1-1 transport Defect	747525	748979	-1	Golgi matrix protein	Golgi matrix protein; involved in the structural organization of the cis-Golgi; interacts genetically with COG3 and USO1
S000005743	YOR217W	RFC1	Replication Factor C	749301	751886	1	Subunit of heteropentameric Replication factor C (RF-C)	Subunit of heteropentameric Replication factor C (RF-C); RF-C is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
S000005744	YOR218C	""	""	751476	751895	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene RFC1/YOR217W
S000005745	YOR219C	STE13	STErile	752214	755009	-1	Dipeptidyl aminopeptidase	Dipeptidyl aminopeptidase; Golgi integral membrane protein that cleaves on the carboxyl side of repeating -X-Ala- sequences, required for maturation of alpha factor, transcription is induced by a-factor
S000005746	YOR220W	RCN2	Regulator of CalciNeurin	755328	756125	1	Protein of unknown function	Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; phosphorylated in response to alpha factor; protein abundance increases in response to DNA replication stress
S000005747	YOR221C	MCT1	Malonyl-CoA:ACP Transferase	756476	757558	-1	Predicted malonyl-CoA:ACP transferase	Predicted malonyl-CoA:ACP transferase; putative component of a type-II mitochondrial fatty acid synthase that produces intermediates for phospholipid remodeling
S000005748	YOR222W	ODC2	OxoDicarboxylate Carrier	758330	759253	1	Mitochondrial inner membrane transporter	Mitochondrial inner membrane transporter; 2-oxodicarboxylate transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for use in lysine and glutamate biosynthesis and in lysine catabolism; ODC2 has a paralog, ODC1, that arose from the whole genome duplication
S000005749	YOR223W	DSC3	Defective for SREBP Cleavage	759782	760660	1	Subunit of the DSC ubiquitin ligase complex	Subunit of the DSC ubiquitin ligase complex; protein of unknown function that localizes to the ER and vacuole lumen; overexpression affects endocytic protein trafficking; ortholog of fission yeast dsc3
S000005750	YOR224C	RPB8	RNA Polymerase B	760825	761265	-1	RNA polymerase subunit ABC14.5	RNA polymerase subunit ABC14.5; common to RNA polymerases I, II, and III
S000005751	YOR225W	""	""	761392	761721	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000005752	YOR226C	ISU2	IscU homolog	761614	762084	-1	Mitochondrial protein required for iron-sulfur protein synthesis	Mitochondrial protein required for iron-sulfur protein synthesis; performs scaffolding function during Fe/S cluster assembly; involved in Fe-S cluster assembly for both mitochondrial and cytosolic proteins; protein abundance increases under DNA replication stress; ISU2 has a paralog, ISU1, that arose from the whole genome duplication; isu1 isu2 double mutant is inviable; human homolog ISCU implicated in mitochondrial myopathy, can complement isu1 isu2 double mutant
S000005753	YOR227W	HER1	Hmg2p ER Remodeling	762825	766565	1	Protein of unknown function	Protein of unknown function; required for proliferation or remodeling of the ER that is caused by overexpression of Hmg2p; may interact with ribosomes, based on co-purification experiments; HER1 has a paralog, GIP3, that arose from the whole genome duplication
S000005754	YOR228C	MCP1	Mdm10 Complementing Protein	766869	767777	-1	Mitochondrial protein of unknown function involved in lipid homeostas	Mitochondrial protein of unknown function involved in lipid homeostasis; integral membrane protein that localizes to the mitochondrial outer membrane; involved in mitochondrial morphology; interacts genetically with MDM10, and other members of the ERMES complex; contains five predicted transmembrane domains
S000005755	YOR229W	WTM2	WD repeat containing Transcriptional Modulator	768409	769812	1	Transcriptional modulator	Transcriptional modulator; involved in regulation of meiosis, silencing, and expression of RNR genes; involved in response to replication stress; contains WD repeats; relocalizes to the cytosol in response to hypoxia; WTM2 has a paralog, UME1, that arose from the whole genome duplication
S000005756	YOR230W	WTM1	WD repeat containing Transcriptional Modulator	770800	772113	1	Transcriptional modulator	Transcriptional modulator; involved in regulation of meiosis, silencing, and expression of RNR genes; acts as a nuclear anchor to retain the ribonucleotide reductase small subunit heterodimer, Rnr2p-Rnr4p, in the nucleus when cells are not in S phase; contains WD repeats
S000005757	YOR231W	MKK1	Mitogen-activated protein Kinase-Kinase	772601	774127	1	MAPKK involved in the protein kinase C signaling pathway	MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication
S000005758	YOR232W	MGE1	Mitochondrial GrpE	774573	775259	1	Mitochondrial matrix cochaperone	Mitochondrial matrix cochaperone; nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; acts as oxidative sensor to regulate mitochondrial Ssc1p; in presence of oxidative stress, dimeric Mge1p becomes a monomer and unable to regulate Ssc1p function; homolog of E. coli GrpE and human Mge1 (GRPEL1), which also responds to oxidative stress
S000005759	YOR233W	KIN4	KINase	775846	778248	1	Serine/threonine protein kinase	Serine/threonine protein kinase; inhibits the mitotic exit network (MEN) when the spindle position checkpoint (SPOC) is activated; regulates peroxisomal transport and vacuolar inheritance along with Frk1p; protects the peroxisomal Myo2p receptor Inp2p from degradation in mother cells along with Frk1p; localizes asymmetrically to mother cell cortex, spindle pole body and bud neck; KIN4 has a paralog, FRK1, that arose from the whole genome duplication
S000005760	YOR234C	RPL33B	Ribosomal Protein of the Large subunit	778555	779405	-1	Ribosomal 60S subunit protein L33B	Ribosomal 60S subunit protein L33B; rpl33b null mutant exhibits normal growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33B has a paralog, RPL33A, that arose from the whole genome duplication
S000005761	YOR235W	IRC13	Increased Recombination Centers	779870	780184	1	Putative protein of unknown function	Putative protein of unknown function; conserved across S. cerevisiae strains; null mutant displays increased levels of spontaneous Rad52 foci
S000005762	YOR236W	DFR1	DihydroFolate Reductase	780906	781541	1	Dihydrofolate reductase involved in tetrahydrofolate biosynthesis	Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism; mutation is functionally complemented by human DHFR
S000005763	YOR237W	HES1	Homologous to kES1	781994	783298	1	Protein implicated in the regulation of ergosterol biosynthesis	Protein implicated in the regulation of ergosterol biosynthesis; one of a seven member gene family with a common essential function and non-essential unique functions; similar to human oxysterol binding protein (OSBP); SWAT-GFP and mCherry fusion proteins localize to the bud neck and vacuolar membrane; HES1 has a paralog, KES1, that arose from the whole genome duplication
S000005764	YOR238W	""	""	783677	784588	1	Putative protein of unknown function	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
S000005765	YOR239W	ABP140	Actin Binding Protein	784857	786744	1	AdoMet-dependent tRNA methyltransferase and actin binding protein	AdoMet-dependent tRNA methyltransferase and actin binding protein; C-terminal domain is responsible for 3-methylcytidine modification of residue 32 of the tRNA anticodon loop of tRNA-Thr and tRNA-Ser and contains an S-adenosylmethionine (AdoMet) binding motif; N-terminal actin binding sequence interacts with actin filaments and localizes to actin patches and cables; N- and C-terminal domains are encoded in separate ORFs that are translated into one protein via a +1 frameshift
S000005766	YOR240W	""	""	785654	786742	1	Merged open reading frame	Merged open reading frame; does not encode a discrete protein; YOR240W was originally annotated as an independent ORF, but was later demonstrated to be part of an adjacent ORF, YOR239W, via a +1 translational frameshift
S000005767	YOR241W	MET7	METhionine requiring	786995	788641	1	Folylpolyglutamate synthetase	Folylpolyglutamate synthetase; catalyzes extension of the glutamate chains of the folate coenzymes, required for methionine synthesis and for maintenance of mitochondrial DNA; protein abundance increases in response to DNA replication stress
S000005768	YOR242C	SSP2	Sporulation SPecific	788742	789857	-1	Sporulation specific protein that localizes to the spore wall	Sporulation specific protein that localizes to the spore wall; required for sporulation at a point after meiosis II and during spore wall formation; expression controlled by a tightly regulated middle-meiotic promoter that is activated by Ndt80p; translation of SSP2 mRNA is delayed, such that the mRNA is present as nuclear divisions are taking place but is not engaged by ribosomes until relatively late in meiotic development
S000005769	YOR243C	PUS7	PseudoUridine Synthase	790211	792241	-1	Pseudouridine synthase	Pseudouridine synthase; catalyzes pseudouridylation at positions 35 and 56 in U2 snRNA, position 50 in 5S rRNA, position 13 in cytoplasmic tRNAs, and position 35 in pre-tRNA(Tyr); also pseudouridylates some mRNAs; relocates from nucleus to cytoplasm during heat shock and differentially modifies some mRNAs during heat shock; conserved in archaea, vertebrates, and some bacteria
S000005770	YOR244W	ESA1	""	792531	793868	1	Catalytic subunit of the histone acetyltransferase complex (NuA4)	Catalytic subunit of the histone acetyltransferase complex (NuA4); acetylates four conserved internal lysines of histone H4 N-terminal tail and can acetylate histone H2A; master regulator of cellular acetylation balance; required for cell cycle progression and transcriptional silencing at the rDNA locus and regulation of autophagy; human ortholog TIP60/KAT5 is implicated in cancer and other diseases, functionally complements lethality of the esa1 null mutation
S000005771	YOR245C	DGA1	DiacylGlycerol Acyltransferase	794076	795332	-1	Diacylglycerol acyltransferase	Diacylglycerol acyltransferase; catalyzes the terminal step of triacylglycerol (TAG) formation, acylates diacylglycerol using acyl-CoA as an acyl donor; Lro1p and Dga1p can O-acylate ceramides; localized to lipid particles
S000005772	YOR246C	ENV9	late ENdosome and Vacuole interface function	795801	796793	-1	Conserved oxidoreductase involved in lipid droplet morphology	Conserved oxidoreductase involved in lipid droplet morphology; mutant shows defects in CPY processing and vacuolar morphology; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts; homologus to human RDH12 linked to Leber Congenital Amaurosis
S000005773	YOR247W	SRL1	Suppressor of Rad53 null Lethality	797676	798308	1	Mannoprotein that exhibits a tight association with the cell wall	Mannoprotein that exhibits a tight association with the cell wall; required for cell wall stability in the absence of GPI-anchored mannoproteins; has a high serine-threonine content; expression is induced in cell wall mutants; SRL1 has a paralog, SVS1, that arose from the whole genome duplication
S000005774	YOR248W	""	""	798013	798315	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000005775	YOR249C	APC5	Anaphase Promoting Complex	798674	800731	-1	Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C)	Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to nuclear foci decreases upon DNA replication stress
S000005777	YOR251C	TUM1	ThioUridine Modification	802550	803464	-1	Rhodanese domain sulfur transferase	Rhodanese domain sulfur transferase; accepts persulfite from Nfs1p and transfers it to Uba4p in the pathway for 2-thiolation of the wobble uridine base of tRNAs; also stimulates sulfur transfer by Nfs1p; involved in metabolism of sterol esters; may be mitochondrially localized
S000005778	YOR252W	TMA16	Translation Machinery Associated	803667	804203	1	Protein of unknown function that associates with ribosomes	Protein of unknown function that associates with ribosomes
S000005779	YOR253W	NAT5	N-terminal AcetylTransferase	804377	804907	1	Subunit of protein N-terminal acetyltransferase NatA	Subunit of protein N-terminal acetyltransferase NatA; NatA is comprised of Nat1p, Ard1p, and Nat5p; N-terminally acetylates many proteins, which influences multiple processes such as the cell cycle, heat-shock resistance, mating, sporulation, and telomeric silencing
S000005780	YOR254C	SEC63	SECretory	805032	807023	-1	Essential subunit of Sec63 complex	Essential subunit of Sec63 complex; with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER; other members are Sec62p, Sec66p, and Sec72p
S000005781	YOR255W	OSW1	Outer Spore Wall	807271	808107	1	Protein involved in sporulation	Protein involved in sporulation; required for the construction of the outer spore wall layers; required for proper localization of Spo14p
S000005782	YOR256C	TRE2	Transferrrin REceptor like	808254	810683	-1	Transferrin receptor-like protein	Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitination and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication
S000005783	YOR257W	CDC31	Cell Division Cycle	811008	811493	1	Calcium-binding component of the spindle pole body (SPB) half-bridge	Calcium-binding component of the spindle pole body (SPB) half-bridge; required for SPB duplication in mitosis and meiosis II; homolog of mammalian centrin; binds multiubiquitinated proteins and is involved in proteasomal protein degradation
S000005784	YOR258W	HNT3	Histidine triad NucleoTide-binding	811671	812324	1	DNA 5' AMP hydrolase involved in DNA repair	DNA 5' AMP hydrolase involved in DNA repair; member of the histidine triad (HIT) superfamily of nucleotide-binding proteins; homolog of Aprataxin, a Hint related protein that is mutated in individuals with ataxia with oculomotor apraxia; relative distribution to nuclear foci decreases upon DNA replication stress
S000005786	YOR260W	GCD1	General Control Derepressed	813984	815720	1	Gamma subunit of the translation initiation factor eIF2B	Gamma subunit of the translation initiation factor eIF2B; the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
S000005787	YOR261C	RPN8	Regulatory Particle Non-ATPase	815915	816931	-1	Essential non-ATPase regulatory subunit of the 26S proteasome	Essential non-ATPase regulatory subunit of the 26S proteasome; has similarity to the human p40 proteasomal subunit and to another S. cerevisiae regulatory subunit, Rpn11p
S000005788	YOR262W	GPN2	Gly-Pro-Asn (N) motif	817292	818335	1	Putative GTPase with a specific role in RNA pol II and polIII biogenes	Putative GTPase with a specific role in RNA pol II and polIII biogenesis; involved in the assembly and subsequent nuclear import of RNA polymerases II and III; required for establishment of sister chromatid cohesion; contains a Gly-Pro-Asn motif in the G domain; similar to Npa3p and Gpn3p; highly conserved across species and homologous to human gene GPN2/ATPBD1B
S000005789	YOR263C	""	""	818757	819164	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF DES3/YOR264W
S000005790	YOR264W	DSE3	Daughter Specific Expression	818866	820158	1	Daughter cell-specific protein, may help establish daughter fate	Daughter cell-specific protein, may help establish daughter fate; relocalizes from bud neck to cytoplasm upon DNA replication stress; interacts with Csm1p
S000005791	YOR265W	RBL2	Rescues Beta-tubulin Lethality	820454	820774	1	Protein involved in microtubule morphogenesis	Protein involved in microtubule morphogenesis; required for protection from excess free beta-tubulin; proposed to be involved the folding of beta-tubulin; similar to mouse beta-tubulin cofactor A; protein abundance increases in response to DNA replication stress
S000005792	YOR266W	PNT1	PeNTamidine resistance	821023	822294	1	Mitochondrial integral inner membrane protein	Mitochondrial integral inner membrane protein; involved in membrane insertion of C-terminus of Cox2p, interacts genetically and physically with Cox18p; deletion mutant sensitive to the anti-Pneumocystis carinii drug pentamidine
S000005793	YOR267C	HRK1	Hygromycin Resistance Kinase	822588	824867	-1	Protein kinase	Protein kinase; implicated in activation of the plasma membrane H(+)-ATPase Pma1p in response to glucose metabolism; plays a role in ion homeostasis; protein abundance increases in response to DNA replication stress
S000005794	YOR268C	""	""	825534	825932	-1	Putative protein of unknown function	Putative protein of unknown function; sporulation is abnormal in homozygous diploid; SWAT-GFP fusion protein localizes to the nucleus; YOR268C is not an essential gene
S000005795	YOR269W	PAC1	Perish in the Absence of Cin8p	826385	827869	1	Regulator of dynein, involved in intracellular transport	Regulator of dynein, involved in intracellular transport; part of the dynein/dynactin pathway; targets dynein to microtubule tips, which is necessary for sliding of microtubules along bud cortex; serves at interface between dynein's ATPase site and its microtubule binding stalk, causing individual dynein motors to remain attached to microtubules for long periods; synthetic lethal with bni1; homolog of human LIS1, mutations in which cause the severe brain disorder lissencephaly
S000005796	YOR270C	VPH1	Vacuolar pH	828052	830574	-1	Subunit a of the vacuolar-ATPase V0 domain	Subunit a of the vacuolar-ATPase V0 domain; encodes one of two isoforms, located in vacuolar V-ATPase complexes while STV1 encodes the second isoform and is located in Golgi and endosomal V-ATPase complexes; interaction with PI(3,5)P2 increases V-ATPase activity and contributes to hyperosmotic stress tolerance; relative distribution to the vacuolar membrane decreases upon DNA replication stress; human homolog ATP6V0A4 implicated in renal tubular acidosis, can complement yeast null mutant
S000005797	YOR271C	FSF1	Fungal SideroFlexin	831059	832042	-1	Putative protein	Putative protein; predicted to be an alpha-isopropylmalate carrier; belongs to the sideroblastic-associated protein family; non-tagged protein is detected in purified mitochondria; likely to play a role in iron homeostasis
S000005798	YOR272W	YTM1	""	832813	834195	1	Ribosomal assembly factor and 66S pre-ribosomal particle constituent	Ribosomal assembly factor and 66S pre-ribosomal particle constituent; subunit of the Nop7-subcomplex (PeBoW complex), required for an early nucleolar step in pre-60S ribosomal particle maturation; interaction of its ubiquitin-like (UBL) domain with the MIDAS domain in the Rea1p tail triggers release of the subcomplex and possibly other biogenesis factors via cycles of ATP hydrolysis; involved in the processing of 27S pre-rRNA; contains an N-terminal UBL domain and seven C-terminal WD repeats
S000005799	YOR273C	TPO4	Transporter of POlyamines	834452	836431	-1	Polyamine transporter of the major facilitator superfamily	Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; localizes to the plasma membrane
S000005800	YOR274W	MOD5	tRNA MODification	837674	838960	1	Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase	Delta 2-isopentenyl pyrophosphate:tRNA isopentenyl transferase; required for biosynthesis of isopentenyladenosine in mitochondrial and cytoplasmic tRNAs; also has a role in tRNA gene-mediated silencing; gene encodes two isozymic forms; converts to a prion form, prion conversion contributes to azole antifungal resistance by upregulating ergosterol biosynthesis; homolog of human TRIT1, a mutation in which is associated with severe combined respiratory chain defects
S000005801	YOR275C	RIM20	Regulator of IME2	839084	841069	-1	Protein involved in proteolytic activation of Rim101p	Protein involved in proteolytic activation of Rim101p; part of response to alkaline pH; PalA/AIP1/Alix family member; interaction with the ESCRT-III subunit Snf7p suggests a relationship between pH response and multivesicular body formation; localizes to cytosol, nucleus and peroxisomes
S000005802	YOR276W	CAF20	Cap Associated Factor	841333	841818	1	Phosphoprotein of the mRNA cap-binding complex	Phosphoprotein of the mRNA cap-binding complex; involved in translational control; repressor of cap-dependent translational initiation; translational activator of select mRNAs during filamentous growth; competes with eIF4G for binding to eIF4E 
S000005803	YOR277C	""	""	841515	841823	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps the verified gene CAF20
S000005804	YOR278W	HEM4	HEMe biosynthesis	842817	843644	1	Uroporphyrinogen III synthase	Uroporphyrinogen III synthase; catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria
S000005805	YOR279C	RFM1	Repression Factor of Middle sporulation element	843698	844630	-1	Component of the Sum1p-Rfm1p-Hst1p complex	Component of the Sum1p-Rfm1p-Hst1p complex; Rfm1p tethers the Hst1p histone deacetylase to the DNA-binding protein Sum1p; complex is involved in transcriptional repression of middle sporulation genes and in initiation of DNA replication
S000005806	YOR280C	FSH3	Family of Serine Hydrolases	844992	845792	-1	Putative serine hydrolase	Putative serine hydrolase; likely target of Cyc8p-Tup1p-Rfx1p transcriptional regulation; localizes to cytosol and peroxisomes; similar to S. cerevisiae Fsh1p and Fsh2p and the human candidate tumor suppressor OVCA2
S000005807	YOR281C	PLP2	Phosducin-Like Protein	846269	847129	-1	Protein that interacts with the CCT complex to stimulate actin folding	Protein that interacts with the CCT complex to stimulate actin folding; has similarity to phosducins; null mutant lethality is complemented by mouse phosducin-like protein MgcPhLP; CCT is short for chaperonin containing TCP-1; essential gene
S000005809	YOR283W	""	""	847453	848145	1	Phosphatase with a broad substrate specificity	Phosphatase with a broad substrate specificity; has some similarity to GPM1/YKL152C, a phosphoglycerate mutase; YOR283W is not an essential gene
S000005810	YOR284W	HUA2	""	848478	849209	1	Cytoplasmic protein of unknown function	Cytoplasmic protein of unknown function; computational analysis of large-scale protein-protein interaction data suggests a possible role in actin patch assembly
S000005811	YOR285W	RDL1	RhoDanese-Like protein	849635	850054	1	Thiosulfate sulfurtransferase	Thiosulfate sulfurtransferase; contains a rhodanese-like domain; localized to the mitochondrial outer membrane; protein abundance increases in response to DNA replication stress; similar to the human TSTD gene
S000005812	YOR286W	RDL2	RhoDanese-Like protein	850280	850729	1	Protein with rhodanese activity	Protein with rhodanese activity; contains a rhodanese-like domain similar to Rdl1p, Uba4p, Tum1p, and Ych1p; overexpression causes a cell cycle delay; null mutant displays elevated frequency of mitochondrial genome loss
S000005813	YOR287C	RRP36	Ribosomal RNA Processing	850937	851839	-1	Component of 90S preribosomes	Component of 90S preribosomes; involved in early cleavages of the 35S pre-rRNA and in production of the 40S ribosomal subunit
S000005814	YOR288C	MPD1	Multicopy suppressor of PDI1 deletion	852121	853077	-1	Member of the protein disulfide isomerase (PDI) family	Member of the protein disulfide isomerase (PDI) family; interacts with and inhibits the chaperone activity of Cne1p; MPD1 overexpression in a pdi1 null mutant suppresses defects in Pdi1p functions such as carboxypeptidase Y maturation
S000005815	YOR289W	""	""	853357	854112	1	Putative protein of unknown function	Putative protein of unknown function; transcription induced by the unfolded protein response; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
S000005816	YOR290C	SNF2	Sucrose NonFermenting	855147	860258	-1	Catalytic subunit of the SWI/SNF chromatin remodeling complex	Catalytic subunit of the SWI/SNF chromatin remodeling complex; involved in transcriptional regulation; contains DNA-stimulated ATPase activity; functions interdependently in transcriptional activation with Snf5p and Snf6p
S000005817	YOR291W	YPK9	Yeast ParK9	861175	865593	1	Vacuolar protein	Vacuolar protein; involved in oxidative stress response and modulation of replicative lifespan; targeted to vacuole via AP-3 pathway; undergoes autophosphorylation, which is abolished by mutation of Asp781 in catalytic motif (D781N); P5 ATPase subfamily member with similarity to yeast Spf1p and homology to human ATP13A2 (PARK9), mutations in which are associated with Parkinson disease and Kufor-Rakeb syndrome
S000005818	YOR292C	""	""	865653	866582	-1	Putative protein of unknown function	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; YOR292C is not an essential gene
S000005819	YOR293W	RPS10A	Ribosomal Protein of the Small subunit	867098	867852	1	Protein component of the small (40S) ribosomal subunit	Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S10, no bacterial homolog; RPS10A has a paralog, RPS10B, that arose from the whole genome duplication; mutations in the human homolog associated with Diamond-Blackfan anemia
S000005820	YOR294W	RRS1	Regulator of Ribosome Synthesis	868340	868951	1	Essential protein that binds ribosomal protein L11	Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice
S000005821	YOR295W	UAF30	Upstream Activation Factor subunit	869208	869894	1	Subunit of UAF (upstream activation factor) complex	Subunit of UAF (upstream activation factor) complex; UAF is an RNA polymerase I specific transcription stimulatory factor composed of Uaf30p, Rrn5p, Rrn9p, Rrn10p, histones H3 and H4; targeting factor for the UAF that facilitates activation of many rDNA genes; deletion decreases cellular growth rate; UAF30 has a paralog, TRI1, that arose from the whole genome duplication
S000005822	YOR296W	""	""	870202	874071	1	Putative protein of unknown function	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; expressed during copper starvation; YOR296W is not an essential gene
S000005823	YOR297C	TIM18	Translocase of the Inner Mitochondrial membrane	874743	875321	-1	Component of the mitochondrial TIM22 complex	Component of the mitochondrial TIM22 complex; involved in insertion of polytopic proteins into the inner membrane; may mediate assembly or stability of the complex
S000005824	YOR298W	MUM3	MUddled Meiosis	875599	877038	1	Protein of unknown function involved in outer spore wall organization	Protein of unknown function involved in outer spore wall organization; has similarity to the tafazzins superfamily of acyltransferases
S000005825	YOR299W	BUD7	BUD site selection	878435	880675	1	Member of the ChAPs family (Chs5p-Arf1p-binding proteins)	Member of the ChAPs family (Chs5p-Arf1p-binding proteins); members include Bch1p, Bch2p, Bud7p, and Chs6p; ChAPs family proteins form the exomer complex with Chs5p to mediate export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; BUD7 has a paralog, BCH1, that arose from the whole genome duplication
S000005826	YOR300W	""	""	880575	880883	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps with verified gene BUD7/YOR299W; mutation affects bipolar budding and bud site selection, though phenotype could be due to the mutation's effects on BUD7
S000005827	YOR301W	RAX1	Revert to Axial	880965	882272	1	Protein involved in establishing bud site selection	Protein involved in establishing bud site selection; localizes to the bud neck and previous sites of cell division (bud scars or cytokinesis remnants (CRMs); localization to both sites is interdependent with Rax2p; required with Rax2p to anchor Nba1p and Nis1p to CRMs where a Cdc42p inhibitory zone is established, preventing repolarization of cells at previous division sites; predicted type IIIa transmembrane protein with similarity to members of the insulin-related peptide superfamily
S000005828	YOR302W	""	""	882765	882842	1	CPA1 uORF	CPA1 uORF; Arginine attenuator peptide, regulates translation of the CPA1 mRNA
S000005829	YOR303W	CPA1	Carbamyl Phosphate synthetase A	882899	884134	1	Small subunit of carbamoyl phosphate synthetase	Small subunit of carbamoyl phosphate synthetase; carbamoyl phosphate synthetase catalyzes a step in the synthesis of citrulline, an arginine precursor; translationally regulated by an attenuator peptide encoded by YOR302W within the CPA1 mRNA 5'-leader
S000005830	YOR304C-A	BIL1	Bud6-Interacting Ligand	888520	888750	-1	Protein that binds Bud6p and has a role in actin cable assembly	Protein that binds Bud6p and has a role in actin cable assembly; involved in the Bnr1p-dependent pathway of cable assembly; localizes to bud tip and bud neck
S000005831	YOR304W	ISW2	Imitation SWitch subfamily	884514	887876	1	ATP-dependent DNA translocase involved in chromatin remodeling	ATP-dependent DNA translocase involved in chromatin remodeling; ATPase component that, with Itc1p, forms a complex required for repression of a-specific genes, INO1, and early meiotic genes during mitotic growth; exhibits basal levels of chromatin binding throughout the genome as well as interacts with sequence-specific factors to accurately position nucleosomes in chromatin; targeted by Ume6p- and Sua7p-dependent DNA looping to many loci genome-wide
S000005832	YOR305W	RRG7	Required for Respiratory Growth	889022	889750	1	Protein of unknown function	Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO); YOR305W is not an essential gene
S000005833	YOR306C	MCH5	MonoCarboxylate transporter Homolog	889867	891432	-1	Plasma membrane riboflavin transporter	Plasma membrane riboflavin transporter; facilitates the uptake of vitamin B2; required for FAD-dependent processes; sequence similarity to mammalian monocarboxylate permeases, however mutants are not deficient in monocarboxylate transport
S000005834	YOR307C	SLY41	Suppressor of Loss of Ypt1	892731	894092	-1	Protein involved in ER-to-Golgi transport	Protein involved in ER-to-Golgi transport; packaged into COPII vesicles for trafficking between ER and Golgi
S000005835	YOR308C	SNU66	Small NUclear ribonucleoprotein associated	894624	896387	-1	Component of the U4/U6.U5 snRNP complex	Component of the U4/U6.U5 snRNP complex; involved in pre-mRNA splicing via spliceosome; also required for pre-5S rRNA processing and may act in concert with Rnh70p; has homology to human SART-1
S000005836	YOR309C	""	""	896698	897078	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58
S000005837	YOR310C	NOP58	NucleOlar Protein of 58 kDa	896825	898360	-1	Protein involved in producing mature rRNAs and snoRNAs	Protein involved in producing mature rRNAs and snoRNAs; involved in pre-rRNA processing, 18S rRNA synthesis, and snoRNA synthesis; component of the small subunit processome complex, which is required for processing of pre-18S rRNA
S000005838	YOR311C	DGK1	DiacylGlycerol Kinase	899056	899928	-1	Diacylglycerol kinase	Diacylglycerol kinase; localized to the endoplasmic reticulum (ER); overproduction induces enlargement of ER-like membrane structures, stimulates the mevalonate/ergosterol pathway and suppresses a temperature-sensitive sly1 mutation; contains a CTP transferase domain
S000005839	YOR312C	RPL20B	Ribosomal Protein of the Large subunit	900250	901194	-1	Ribosomal 60S subunit protein L20B	Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication
S000005840	YOR313C	SPS4	SPorulation Specific trancript	901858	902874	-1	Protein whose expression is induced during sporulation	Protein whose expression is induced during sporulation; not required for sporulation; heterologous expression in E. coli induces the SOS response that senses DNA damage
S000005841	YOR314W	""	""	903043	903372	1	Putative protein of unknown function	Putative protein of unknown function; conserved across S. cerevisiae strains
S000005842	YOR315W	SFG1	SuperFicial pseudohyphal Growth	904760	905800	1	Putative transcription factor	Putative transcription factor; induces superficial pseudohyphal growth, positively regulates invasive growth, but is not required for invasive pseudohyphal growth; may act together with Phd1p; promotes cell adhesion independent of Flo11p by repressing genes that encode cell wall degrading enzymes; localizes to the nucleus; potential Cdc28p substrate
S000005843	YOR316C	COT1	CObalt Toxicity	906236	907555	-1	Vacuolar transporter that mediates zinc transport into vacuole	Vacuolar transporter that mediates zinc transport into vacuole; overexpression confers resistance to cobalt and rhodium; targeted to vacuole via AP-3 pathway; protein abundance increases in response to DNA replication stress; COT1 has a paralog, ZRC1, that arose from the whole genome duplication
S000005844	YOR317W	FAA1	Fatty Acid Activation	909343	911445	1	Long chain fatty acyl-CoA synthetase	Long chain fatty acyl-CoA synthetase; activates fatty acids with a preference for C12:0-C16:0 chain lengths; role in the competitive import of long-chain fatty acids and sphingoid long-chain bases; accounts for most acyl-CoA synthetase activity; localizes to lipid particles and the plasma membrane; role in sphingolipid-to-glycerolipid metabolism; forms ER foci upon replication stress; faa1 faa4 double null complemented by any of human ACSBG1, ACSL1, 3, 4, 5, 6, SLC27A2, or 4
S000005845	YOR318C	""	""	911784	912436	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; transcript is predicted to be spliced but there is no evidence that it is spliced in vivo
S000005846	YOR319W	HSH49	Human Sap Homolog	912822	913463	1	U2-snRNP associated splicing factor	U2-snRNP associated splicing factor; similar to the mammalian splicing factor SAP49; proposed to function as a U2-snRNP assembly factor along with Hsh155p and binding partner Cus1p; contains two RNA recognition motifs (RRM)
S000005847	YOR320C	GNT1	GlcNAc Transferase	913619	915094	-1	N-acetylglucosaminyltransferase	N-acetylglucosaminyltransferase; capable of modification of N-linked glycans in the Golgi apparatus
S000005848	YOR321W	PMT3	Protein O-MannosylTransferase	916030	918291	1	Protein O-mannosyltransferase	Protein O-mannosyltransferase; transfers mannose residues from dolichyl phosphate-D-mannose to protein serine/threonine residues; acts in a complex with Pmt5p, can instead interact with Pmt1p in some conditions; antifungal drug target; PMT3 has a paralog, PMT2, that arose from the whole genome duplication
S000005849	YOR322C	LDB19	Low Dye Binding	918606	921062	-1	Alpha-arrestin, Ub-ligase adaptor for Rsp5p	Alpha-arrestin, Ub-ligase adaptor for Rsp5p; regulates starvation- and substrate-induced Ub-dependent endocytosis of select plasma membrane localized amino acid transporters, recruiting Rsp5p to its targets; role in basal internalization and turnover of Ste2p, mating and zygote formation; recruits Rsp5p to Ste2p via its 2 PPXY motifs; inhibited by Npr1p-mediated phosphorylation, affecting cytosol and plasma membrane translocation; localization regulated by Rsp5p-dependent ubiquitination
S000005850	YOR323C	PRO2	PROline requiring	921535	922905	-1	Gamma-glutamyl phosphate reductase	Gamma-glutamyl phosphate reductase; catalyzes the second step in proline biosynthesis
S000005851	YOR324C	FRT1	Functionally Related to TCP1	923232	925040	-1	Tail-anchored ER membrane protein of unknown function	Tail-anchored ER membrane protein of unknown function; substrate of the phosphatase calcineurin; interacts with homolog Frt2p; promotes cell growth in stress conditions, possibly via a role in posttranslational translocation; FRT1 has a paralog, FRT2, that arose from the whole genome duplication
S000005852	YOR325W	""	""	924577	925050	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified ORF FRT1
S000005853	YOR326W	MYO2	MYOsin	925721	930445	1	Type V myosin motor involved in actin-based transport of cargos	Type V myosin motor involved in actin-based transport of cargos; required for the polarized delivery of secretory vesicles, the vacuole, late Golgi elements, peroxisomes, lipid droplets, and the mitotic spindle; effector of Ypt11p, a rab-type small GTPase; MYO2 has a paralog, MYO4, that arose from the whole genome duplication
S000005854	YOR327C	SNC2	Suppressor of the Null allele of CAP	930734	931081	-1	Vesicle membrane receptor protein (v-SNARE)	Vesicle membrane receptor protein (v-SNARE); involved in the fusion between Golgi-derived secretory vesicles with the plasma membrane; Snc2p levels regulated by Vps45p; member of the synaptobrevin/VAMP family of R-type v-SNARE proteins; SNC2 has a paralog, SNC1, that arose from the whole genome duplication
S000005855	YOR328W	PDR10	Pleiotropic Drug Resistance	931803	936497	1	ATP-binding cassette (ABC) transporter	ATP-binding cassette (ABC) transporter; multidrug transporter involved in the pleiotropic drug resistance network; regulated by Pdr1p and Pdr3p
S000005856	YOR329C	SCD5	Suppressor of Clathrin Deficiency	936731	939349	-1	Protein required for normal actin organization and endocytosis	Protein required for normal actin organization and endocytosis; targeting subunit for protein phosphatase type 1; undergoes Crm1p-dependent nuclear-cytoplasmic shuttling; multicopy suppressor of clathrin deficiency
S000005857	YOR330C	MIP1	MItochondrial DNA Polymerase	939621	943385	-1	Mitochondrial DNA polymerase gamma	Mitochondrial DNA polymerase gamma; single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoan complex of catalytic and accessory subunits; polymorphic in yeast, petites occur more frequently in some lab strains; human ortholog POLG complements yeast mip1 mutant; mutations in human POLG associated with Alpers-Huttenlocher syndrome (AHS), progressive external ophthalmoplegia (PEO), parkinsonism, other mitochondrial diseases
S000005858	YOR331C	""	""	943565	944122	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene VMA4/YOR332W
S000005859	YOR332W	VMA4	Vacuolar Membrane Atpase	943656	944357	1	Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase)	Subunit E of the V1 domain of the vacuolar H+-ATPase (V-ATPase); V-ATPase is an electrogenic proton pump found throughout the endomembrane system; V1 domain has eight subunits; required for the V1 domain to assemble onto the vacuolar membrane; protein abundance increases in response to DNA replication stress
S000005860	YOR333C	""	""	944541	944957	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 5' end of MRS2 gene required for respiratory growth
S000005861	YOR334W	MRS2	Mitochondrial RNA Splicing	944596	946008	1	Mitochondrial inner membrane Mg(2+) channel	Mitochondrial inner membrane Mg(2+) channel; required for maintenance of intramitochondrial Mg(2+) concentrations at the correct level to support splicing of group II introns; similar to bacterial CorA
S000005862	YOR335C	ALA1	ALAnyl-tRNA synthetase	946233	949109	-1	Cytoplasmic and mitochondrial alanyl-tRNA synthetase	Cytoplasmic and mitochondrial alanyl-tRNA synthetase; required for protein synthesis; point mutation (cdc64-1 allele) causes cell cycle arrest at G1; lethality of null mutation is functionally complemented by human homolog AARS; mutations in human homolog AARS are associated with autoimmune disease polymyositis/dermatomyositis and with hereditary peripheral neuropathy, Charcot-Marie-Tooth (CMT) disease
S000005863	YOR336W	KRE5	Killer toxin REsistant	949773	953870	1	Protein required for beta-1,6 glucan biosynthesis	Protein required for beta-1,6 glucan biosynthesis; mutations result in aberrant morphology and severe growth defects
S000005864	YOR337W	TEA1	Ty Enhancer Activator	954344	956623	1	Ty1 enhancer activator involved in Ty enhancer-mediated transcription	Ty1 enhancer activator involved in Ty enhancer-mediated transcription; required for full levels of Ty enhancer-mediated transcription; C6 zinc cluster DNA-binding protein
S000005865	YOR338W	""	""	956898	957989	1	Putative protein of unknown function	Putative protein of unknown function; YOR338W transcription is regulated by Azf1p and its transcript is a specific target of the G protein effector Scp160p; identified as being required for sporulation in a high-throughput mutant screen; YOR338W has a paralog, FUN19, that arose from the whole genome duplication
S000005866	YOR339C	UBC11	UBiquitin Carrier	958362	958832	-1	Ubiquitin-conjugating enzyme	Ubiquitin-conjugating enzyme; most similar in sequence to Xenopus ubiquitin-conjugating enzyme E2-C, but not a true functional homolog of this E2; unlike E2-C, not required for the degradation of mitotic cyclin Clb2
S000005867	YOR340C	RPA43	RNA Polymerase A	959202	960182	-1	RNA polymerase I subunit A43	RNA polymerase I subunit A43
S000005868	YOR341W	RPA190	RNA Polymerase A	960987	965981	1	RNA polymerase I largest subunit A190	RNA polymerase I largest subunit A190
S000005869	YOR342C	""	""	966666	967625	-1	Protein of unknown function	Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; relocalizes from nucleus to cytoplasm upon DNA replication stress; YOR342C has a paralog, YAL037W, that arose from the whole genome duplication
S000005870	YOR343C	""	""	968148	968474	-1	Putative protein of unknown function	Putative protein of unknown function; conserved across S. cerevisiae strains
S000005871	YOR344C	TYE7	Ty1-mediated Expression	977194	978069	-1	Serine-rich protein that contains a bHLH DNA binding motif	Serine-rich protein that contains a bHLH DNA binding motif; binds E-boxes of glycolytic genes and contributes to their activation; may function as a transcriptional activator in Ty1-mediated gene expression; bHLH stands for basic-helix-loop-helix
S000005872	YOR345C	""	""	981812	982162	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps the verified gene REV1; null mutant displays increased resistance to antifungal agents gliotoxin, cycloheximide and H2O2
S000005873	YOR346W	REV1	REVersionless	981828	984785	1	Bifunctional DNA-directed DNA polymerase/deoxycytidyl transferase	Bifunctional DNA-directed DNA polymerase/deoxycytidyl transferase; involved in repair of abasic sites and adducted guanines in damaged DNA by translesion synthesis (TLS); forms a complex with the subunits of DNA polymerase zeta, Rev3p and Rev7p; relocalizes from nucleus to cytoplasm upon DNA replication stress; may be involved in meiosis
S000005874	YOR347C	PYK2	PYruvate Kinase	984942	986462	-1	Pyruvate kinase	Pyruvate kinase; appears to be modulated by phosphorylation; transcription repressed by glucose, and Pyk2p may be active under low glycolytic flux; PYK2 has a paralog, CDC19, that arose from the whole genome duplication
S000005875	YOR348C	PUT4	Proline UTilization	986899	988782	-1	Proline permease	Proline permease; required for high-affinity transport of proline; also transports the toxic proline analog azetidine-2-carboxylate (AzC); PUT4 transcription is repressed in ammonia-grown cells
S000005876	YOR349W	CIN1	Chromosome INstability	989789	992833	1	Tubulin folding factor D involved in beta-tubulin (Tub2p) folding	Tubulin folding factor D involved in beta-tubulin (Tub2p) folding; isolated as mutant with increased chromosome loss and sensitivity to benomyl; CIN1 mRNA level is reduced when downstream gene MNE1 is deleted
S000005877	YOR350C	MNE1	""	992864	994855	-1	Protein involved in splicing Group I aI5-beta intron from COX1 mRNA	Protein involved in splicing Group I aI5-beta intron from COX1 mRNA; mitochondrial matrix protein
S000005878	YOR351C	MEK1	MEiotic Kinase	995018	996511	-1	Meiosis-specific serine/threonine protein kinase	Meiosis-specific serine/threonine protein kinase; functions in meiotic checkpoint, promotes recombination between homologous chromosomes by suppressing double strand break repair between sister chromatids; stabilizes Hop1-Thr318 phosphorylation to promote interhomolog recombination and checkpoint responses during meiosis
S000005879	YOR352W	TFB6	""	997213	998244	1	Subunit of TFIIH complex	Subunit of TFIIH complex; facilities dissociation of the Ssl2p helices from TFIIH; expression levels regulated by Arg5,6p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
S000005880	YOR353C	SOG2	""	998453	1000828	-1	Key component of the RAM signaling network	Key component of the RAM signaling network; required for proper cell morphogenesis and cell separation after mitosis
S000005881	YOR354C	MSC6	Meiotic Sister-Chromatid recombination	1001147	1003225	-1	Multicopy suppressor of HER2 involved in mitochondrial translation	Multicopy suppressor of HER2 involved in mitochondrial translation; mutant is defective in directing meiotic recombination events to homologous chromatids
S000005882	YOR355W	GDS1	""	1005137	1006705	1	Protein involved in histone H4 acetylation at ribosomal protein genes	Protein involved in histone H4 acetylation at ribosomal protein genes; interacts with and modulates NuA4 histone acetyltransferase complex; required for growth on glycerol as a carbon source; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; contains a segment predicted to resemble the winged-helix/forkhead domain located in DNA-binding proteins
S000005883	YOR356W	CIR2	Changed Intracellular Redox state	1007221	1009116	1	Putative ortholog of human ETF-dH	Putative ortholog of human ETF-dH; found in a large supramolecular complex with other mitochondrial dehydrogenases; may have a role in oxidative stress response; ETF-dH is also known as electron transfer flavoprotein dehydrogenase
S000005884	YOR357C	SNX3	Sorting NeXin	1009224	1009712	-1	Sorting nexin for late-Golgi enzymes	Sorting nexin for late-Golgi enzymes; required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p
S000005885	YOR358W	HAP5	Heme Activator Protein	1010161	1010889	1	Subunit of the Hap2p/3p/4p/5p CCAAT-binding complex	Subunit of the Hap2p/3p/4p/5p CCAAT-binding complex; complex is heme-activated and glucose repressed; complex is a transcriptional activator and global regulator of respiratory gene expression; required for assembly and DNA binding activity of the complex
S000005886	YOR359W	VTS1	VTi1-2 Suppressor	1011189	1012760	1	Flap-structured DNA-binding and RNA-binding protein	Flap-structured DNA-binding and RNA-binding protein; stimulates deadenylation-dependent mRNA degradation mediated by the CCR4-NOT deadenylase complex; member of the Smaug (Smg) family of post-transcriptional regulators which bind RNA through a conserved sterile alpha motif (SAM) domain that interacts with Smg recognition element (SREs) containing transcripts; stimulates Dna2p endonuclease activity; forms [SMAUG+] prion
S000005887	YOR360C	PDE2	PhosphoDiEsterase	1013241	1014821	-1	High-affinity cyclic AMP phosphodiesterase	High-affinity cyclic AMP phosphodiesterase; component of the cAMP-dependent protein kinase signaling system, protects the cell from extracellular cAMP, contains readthrough motif surrounding termination codon
S000005888	YOR361C	PRT1	PRoTein synthesis	1015361	1017652	-1	eIF3b subunit of the eukaryotic translation initiation factor 3 (eIF3)	eIF3b subunit of the eukaryotic translation initiation factor 3 (eIF3); subunit of the core complex of eIF3; essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes; eIF3 is also involved in programmed stop codon readthrough
S000005890	YOR363C	PIP2	Peroxisome Induction Pathway	1020222	1023212	-1	Autoregulatory, oleate-activated transcription factor	Autoregulatory, oleate-activated transcription factor; subunit of a heterodimeric complex with Oaf1p, which binds to oleate-response elements (ORE) in the promoter of genes involved in beta-oxidation of fatty acids, peroxisome organization and biogenesis, activating transcription in the presence of oleate; PIP2 has a paralog, OAF1, that arose from the whole genome duplication
S000005891	YOR364W	""	""	1023371	1023739	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR365C
S000005892	YOR365C	""	""	1023461	1025572	-1	Putative protein involved in sphingolipid biosynthesis	Putative protein involved in sphingolipid biosynthesis; not an essential gene; YOR365C has a paralog, FLC2, that arose from the whole genome duplication
S000005893	YOR366W	""	""	1025256	1025609	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR365C
S000005894	YOR367W	SCP1	S. cerevisiae CalPonin	1026007	1026609	1	Component of yeast cortical actin cytoskeleton	Component of yeast cortical actin cytoskeleton; binds and cross links actin filaments; originally identified by its homology to calponin (contains a calponin-like repeat) but the Scp1p domain structure is more similar to transgelin
S000005895	YOR368W	RAD17	RADiation sensitive	1026843	1028048	1	Checkpoint protein	Checkpoint protein; involved in the activation of the DNA damage and meiotic pachytene checkpoints; with Mec3p and Ddc1p, forms a clamp that is loaded onto partial duplex DNA; homolog of human and S. pombe Rad1 and U. maydis Rec1 proteins
S000005896	YOR369C	RPS12	Ribosomal Protein of the Small subunit	1028194	1028625	-1	Protein component of the small (40S) ribosomal subunit	Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S12, no bacterial homolog
S000005897	YOR370C	MRS6	Mitochondrial RNA Splicing	1029183	1030994	-1	Rab escort protein	Rab escort protein; type II geranylgeranyltransferase complex (Bet2p-Bet4p) chaperone that complexes with newly synthesized Rab GTPases, like Ypt1p and Sec4p, leading to their prenylation and membrane delivery; modulates the TOR pathway through interactions with Sfp1p; alters the kinetics of MAPK pathway activation and polarity reorganization during filamentous growth; sequence similarity to mammalian choroideraemia gene; relative distribution to the nucleus increases upon DNA replication stress
S000005898	YOR371C	GPB1	""	1031489	1034182	-1	Multistep regulator of cAMP-PKA signaling	Multistep regulator of cAMP-PKA signaling; inhibits PKA downstream of Gpa2p and Cyr1p, thereby increasing cAMP dependency; promotes ubiquitin-dependent proteolysis of Ira2p; regulated by G-alpha protein Gpa2p; GPB1 has a paralog, GPB2, that arose from the whole genome duplication
S000005900	YOR373W	NUD1	""	1036834	1039389	1	Mitotic exit network (MEN) scaffold protein	Mitotic exit network (MEN) scaffold protein; core component of the SPB outer plaque that functions in astral microtubule organization via Spc72p; scaffold for recruitment of MEN components, such as Cdc15p, Tem1p and the Bfa1p-Bub2p checkpoint complex; phosphorylation by Cdc15p creates a Dbf2p-Mob1p phospho-docking site enabling Cdc15p-dependent complex activation and mitotic exit; acts through the MEN to specify Kar9p-mediated asymmetric SPB inheritance; homologous to mammalian centriolin
S000005901	YOR374W	ALD4	ALdehyde Dehydrogenase	1039840	1041399	1	Mitochondrial aldehyde dehydrogenase	Mitochondrial aldehyde dehydrogenase; required for growth on ethanol and conversion of acetaldehyde to acetate; phosphorylated; activity is K+ dependent; utilizes NADP+ or NAD+ equally as coenzymes; expression is glucose repressed; can substitute for cytosolic NADP-dependent aldehyde dehydrogenase when directed to the cytosol; human homolog ALDH2 can complement yeast ald4 mutant
S000005902	YOR375C	GDH1	Glutamate DeHydrogenase	1041678	1043042	-1	NADP(+)-dependent glutamate dehydrogenase	NADP(+)-dependent glutamate dehydrogenase; synthesizes glutamate from ammonia and alpha-ketoglutarate; rate of alpha-ketoglutarate utilization differs from Gdh3p; expression regulated by nitrogen and carbon sources; GDH1 has a paralog, GDH3, that arose from the whole genome duplication
S000005903	YOR376W	""	""	1043191	1043559	1	Putative protein of unknown function	Putative protein of unknown function; conserved among S. cerevisiae strains; YOR376W is not an essential gene
S000005904	YOR377W	ATF1	AcetylTransFerase	1046226	1047803	1	Alcohol acetyltransferase	Alcohol acetyltransferase; responsible for the major part of volatile acetate ester production during fermentation; main enzyme involved in terpenyl acetate synthesis; potential roles in lipid and sterol metabolism
S000005905	YOR378W	AMF1	AMmonium Facilitator	1049511	1051058	1	Low affinity NH4+ transporter	Low affinity NH4+ transporter; member of the DHA2 family of drug:H+ anti porters; putative paralog of ATR1; but not required for boron tolerance; non-essential gene
S000005906	YOR379C	""	""	1050726	1051064	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YOR378W
S000005907	YOR380W	RDR1	Repressor of Drug Resistance	1051290	1052930	1	Transcriptional repressor involved in regulating multidrug resistance	Transcriptional repressor involved in regulating multidrug resistance; negatively regulates expression of the PDR5 gene; member of the Gal4p family of zinc cluster proteins
S000005908	YOR381W	FRE3	Ferric REductase	1055545	1057680	1	Ferric reductase	Ferric reductase; reduces siderophore-bound iron prior to uptake by transporters; expression induced by low iron levels
S000005909	YOR382W	FIT2	Facilitator of Iron Transport	1059531	1059992	1	Mannoprotein that is incorporated into the cell wall	Mannoprotein that is incorporated into the cell wall; incorporated via a glycosylphosphatidylinositol (GPI) anchor; involved in the retention of siderophore-iron in the cell wall 
S000005910	YOR383C	FIT3	Facilitator of Iron Transport	1060441	1061055	-1	Mannoprotein that is incorporated into the cell wall	Mannoprotein that is incorporated into the cell wall; incorporated via a glycosylphosphatidylinositol (GPI) anchor; involved in the retention of siderophore-iron in the cell wall 
S000005911	YOR384W	FRE5	Ferric REductase	1061564	1063648	1	Putative ferric reductase with similarity to Fre2p	Putative ferric reductase with similarity to Fre2p; expression induced by low iron levels; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
S000005912	YOR385W	""	""	1065043	1065915	1	Putative protein of unknown function	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene
S000005913	YOR386W	PHR1	PHotoreactivation Repair deficient	1066839	1068536	1	DNA photolyase involved in photoreactivation	DNA photolyase involved in photoreactivation; repairs pyrimidine dimers in the presence of visible light; induced by DNA damage; regulated by transcriptional repressor Rph1p
S000005922	YPL001W	HAT1	Histone AcetylTransferase	554605	555729	1	Catalytic subunit of the Hat1p-Hat2p histone acetyltransferase complex	Catalytic subunit of the Hat1p-Hat2p histone acetyltransferase complex; uses the cofactor acetyl coenzyme A to acetylate free nuclear and cytoplasmic histone H4; involved in telomeric silencing and DNA double-strand break repair
S000005923	YPL002C	SNF8	Sucrose NonFermenting	553627	554328	-1	Component of the ESCRT-II complex	Component of the ESCRT-II complex; ESCRT-II is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression
S000005924	YPL003W	ULA1	Ubiquitin-Like protein Activation	552020	553408	1	Protein that activates Rub1p (NEDD8) before neddylation	Protein that activates Rub1p (NEDD8) before neddylation; acts together with Uba3p; may play a role in protein degradation
S000005925	YPL004C	LSP1	Long chain bases Stimulate Phosphorylation	550632	551657	-1	Eisosome core component	Eisosome core component; eisosomes are large immobile patch structures at the cell cortex associated with endocytosis; phosphorylated on Thr233 upon Pkc1p hyperactivation in a Slt2p MAPK-dependent fashion; null mutants show activation of Pkc1p/Ypk1p stress resistance pathways; member of the BAR domain family
S000005926	YPL005W	AEP3	ATPase ExPression	548486	550306	1	Peripheral mitochondrial inner membrane protein	Peripheral mitochondrial inner membrane protein; may facilitate use of unformylated tRNA-Met in mitochondrial translation initiation; stabilizes the bicistronic AAP1-ATP6 mRNA
S000005927	YPL006W	NCR1	Niemann-pick type C Related	544631	548143	1	Vacuolar membrane protein involved with Npc2p in sterol trafficking	Vacuolar membrane protein involved with Npc2p in sterol trafficking; transits through biosynthetic vacuolar protein sorting pathway, involved in sphingolipid metabolism; cells lacking Ncr1p exhibit high levels of long chain bases (LCB), similar to accumulation of high amounts of lipids observed in patients with Neimann-Pick C disease caused by loss-of-function mutations in human NPC1; targeted to vacuole via AP-3 pathway
S000005929	YPL008W	CHL1	CHromosome Loss	539385	541970	1	Probable DNA helicase	Probable DNA helicase; involved in sister-chromatid cohesion and genome integrity and interstrand cross-link repair; interacts with ECO1 and CTF18; mutants are defective in silencing, rDNA recombination, aging and the heat shock response; FANCJ-like helicase family member; mutations in the human homolog, DDX11/ChLR1, cause Warsaw breakage syndrome
S000005930	YPL009C	RQC2	Ribosome Quality control Complex	535820	538936	-1	Component of the Ribosome Quality Control (RQC) complex	Component of the Ribosome Quality Control (RQC) complex;  RQC is a ribosome-bound complex required for degradation of polypeptides arising from stalled translation; recruits alanine- and threonine-charged tRNA to the A site and directs the elongation of nascent chains independently of mRNA or 40S subunits; monitors translation stress and signals this to Hsf1p
S000005931	YPL010W	RET3	RETrieval from ER	535018	535587	1	Zeta subunit of the coatomer complex (COPI)	Zeta subunit of the coatomer complex (COPI); COPI coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER
S000005932	YPL011C	TAF3	TATA binding protein-Associated Factor	533642	534703	-1	TFIID subunit (47 kDa)	TFIID subunit (47 kDa); involved in promoter binding and RNA polymerase II transcription initiation
S000005933	YPL012W	RRP12	Ribosomal RNA Processing	529723	533409	1	Protein required for export of the ribosomal subunits	Protein required for export of the ribosomal subunits; associates with the RNA components of the pre-ribosomes; has a role in nuclear import in association with Pse1p; also plays a role in the cell cycle and the DNA damage response; contains HEAT-repeats
S000005934	YPL013C	MRPS16	Mitochondrial Ribosomal Protein, Small subunit	528985	529350	-1	Mitochondrial ribosomal protein of the small subunit	Mitochondrial ribosomal protein of the small subunit
S000005935	YPL014W	CIP1	Cdk1 Interacting Protein	527547	528692	1	Cyclin-dependent kinase inhibitor	Cyclin-dependent kinase inhibitor; activated by environmental stress; interacts with and inhibits the Cdc28p/Cln2p, G1/S phase cyclin-dependent kinase complex but not S-phase, or M-phase complexes; overexpression blocks cells in G1 phase and stabilizes the Cdc28p inhibitor Sic1p, while disruption accelerates the G1/S phase transition; phosphorylated during S phase in a Cdc28p-dependent manner; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus
S000005936	YPL015C	HST2	Homolog of SIR Two (SIR2)	525810	526883	-1	Cytoplasmic NAD(+)-dependent protein deacetylase	Cytoplasmic NAD(+)-dependent protein deacetylase; deacetylation targets are primarily cytoplasmic proteins; member of the silencing information regulator 2 (Sir2) family of NAD(+)-dependent protein deacetylases; modulates nucleolar (rDNA) and telomeric silencing; possesses NAD(+)-dependent histone deacetylase activity in vitro; contains a nuclear export signal (NES); function regulated by its nuclear export
S000005937	YPL016W	SWI1	SWItching deficient	521014	524958	1	Subunit of the SWI/SNF chromatin remodeling complex	Subunit of the SWI/SNF chromatin remodeling complex; regulates transcription by remodeling chromatin; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; self-assembles to form [SWI+] prion and to alter expression pattern; human homolog ARID1A is a candidate tumor suppressor gene in breast cancer
S000005938	YPL017C	IRC15	Increased Recombination Centers	518734	520233	-1	Microtubule associated protein	Microtubule associated protein; regulates microtubule dynamics; required for accurate meiotic chromosome segregation; null mutant displays large budded cells due to delayed mitotic progression, increased levels of spontaneous Rad52 foci; IRC15 has a paralog, LPD1, that arose from the whole genome duplication
S000005939	YPL018W	CTF19	Chromosome Transmission Fidelity	517651	518760	1	Outer kinetochore protein, needed for accurate chromosome segregation	Outer kinetochore protein, needed for accurate chromosome segregation; component of kinetochore sub-complex COMA (Ctf19p, Okp1p, Mcm21p, Ame1p) that functions as platform for kinetochore assembly; required for spindle assembly checkpoint; minimizes potentially deleterious centromere-proximal crossovers by preventing meiotic DNA break formation proximal to centromere; homolog of human centromere constitutive-associated network (CCAN) subunit CENP-P and fission yeast fta2
S000005940	YPL019C	VTC3	Vacuolar Transporter Chaperone	514511	517018	-1	Regulatory subunit of vacuolar transporter chaperone (VTC) complex	Regulatory subunit of vacuolar transporter chaperone (VTC) complex; involved in membrane trafficking, vacuolar polyphosphate accumulation, microautophagy and non-autophagic vacuolar fusion; important regulator of substrate invagination from the vacuolar membrane; targeted to vacuole via AP-3 pathway; VTC3 has a paralog, VTC2, that arose from the whole genome duplication
S000005941	YPL020C	ULP1	UbL-specific Protease	512313	514178	-1	Protease that specifically cleaves Smt3p protein conjugates	Protease that specifically cleaves Smt3p protein conjugates; required for cell cycle progression; associates with nucleoporins and may interact with septin rings during telophase; contributes to regulation of telomeric silencing; sequestered to the nucleolus under stress conditions
S000005942	YPL021W	ECM23	ExtraCellular Mutant	511101	511664	1	Non-essential protein of unconfirmed function	Non-essential protein of unconfirmed function; affects pre-rRNA processing, may act as a negative regulator of the transcription of genes involved in pseudohyphal growth; homologous to Srd1p
S000005943	YPL022W	RAD1	RADiation sensitive	506697	509999	1	Single-stranded DNA endonuclease (with Rad10p)	Single-stranded DNA endonuclease (with Rad10p); cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein
S000005944	YPL023C	MET12	METhionine requiring	504339	506312	-1	Protein with MTHFR activity in vitro	Protein with MTHFR activity in vitro; null mutant has no phenotype and is prototrophic for methionine; MET13 encodes major isozyme of methylenetetrahydrofolate reductase (MTHFR)
S000005945	YPL024W	RMI1	RecQ Mediated genome Instability	503517	504242	1	Subunit of the RecQ (Sgs1p) - Topo III (Top3p) complex	Subunit of the RecQ (Sgs1p) - Topo III (Top3p) complex; stimulates superhelical relaxing, DNA catenation/decatenation and ssDNA binding activities of Top3p; involved in response to DNA damage; functions in S phase-mediated cohesion establishment via a pathway involving the Ctf18-RFC complex and Mrc1p; stimulates Top3p DNA catenation/decatenation activity; null mutants display increased rates of recombination and delayed S phase
S000005946	YPL025C	""	""	502473	503030	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000005947	YPL026C	SKS1	Suppressor Kinase of SNF3	500675	502183	-1	Putative serine/threonine protein kinase	Putative serine/threonine protein kinase; involved in the adaptation to low concentrations of glucose independent of the SNF3 regulated pathway; SKS1 has a paralog, VHS1, that arose from the whole genome duplication
S000005948	YPL027W	SMA1	Spore Membrane Assembly	499665	500402	1	Protein of unknown function involved in prospore membrane assembly	Protein of unknown function involved in prospore membrane assembly; involved in the assembly of the prospore membrane during sporulation; interacts with Spo14p
S000005949	YPL028W	ERG10	ERGosterol biosynthesis	498096	499292	1	Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase)	Acetyl-CoA C-acetyltransferase (acetoacetyl-CoA thiolase); cytosolic enzyme that transfers an acetyl group from one acetyl-CoA molecule to another, forming acetoacetyl-CoA; involved in the first step in mevalonate biosynthesis; human ACAT1 functionally complements the growth defect caused by repression of ERG10 expression
S000005950	YPL029W	SUV3	SUppressor of Var1	495506	497719	1	ATP-dependent RNA helicase	ATP-dependent RNA helicase; component of the mitochondrial degradosome along with the RNase Dss1p; the degradosome associates with the ribosome and mediates RNA turnover; also required during splicing of the COX1 AI5_beta intron; expression of a processed form of human homolog SUPV3L1 carrying an N-terminal deletion of 46 amino acids rescues yeast suv3 null mutant
S000005951	YPL030W	TRM44	TRna Methyltransferase	493543	495246	1	tRNA(Ser) Um(44) 2'-O-methyltransferase	tRNA(Ser) Um(44) 2'-O-methyltransferase; involved in maintaining levels of the tRNA-Ser species tS(CGA) and tS(UGA); conserved among metazoans and fungi but there does not appear to be a homolog in plants; TRM44 is a non-essential gene
S000005952	YPL031C	PHO85	PHOsphate metabolism	492018	493037	-1	Cyclin-dependent kinase	Cyclin-dependent kinase; has ten cyclin partners; involved in regulating the cellular response to nutrient levels and environmental conditions and progression through the cell cycle; human lissencephaly-associated homolog CDK5 functionally complements null mutation
S000005953	YPL032C	SVL3	Styryl dye Vacuolar Localization	488887	491364	-1	Protein of unknown function	Protein of unknown function; mutant phenotype suggests a potential role in vacuolar function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; relocalizes from bud neck to cytoplasm upon DNA replication stress; SVL3 has a paralog, PAM1, that arose from the whole genome duplication
S000005954	YPL033C	SRL4	Suppressor of Rad53 null Lethality or Suppressor of Rad53 and Lcd1	487362	488207	-1	Protein of unknown function	Protein of unknown function; involved in regulation of dNTP production; null mutant suppresses the lethality of lcd1 and rad53 mutations; expression is induced by Kar4p
S000005955	YPL034W	""	""	486712	487209	1	Putative protein of unknown function	Putative protein of unknown function; YPL034W is not essential gene
S000005956	YPL035C	""	""	486564	486911	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized gene YPL034W; YPL035C is not an essential gene
S000005957	YPL036W	PMA2	Plasma Membrane ATPase	482843	485686	1	Plasma membrane H+-ATPase	Plasma membrane H+-ATPase; isoform of Pma1p, involved in pumping protons out of the cell; regulator of cytoplasmic pH and plasma membrane potential
S000005958	YPL037C	EGD1	Enhancer of Gal4 DNA binding	481428	481901	-1	Subunit beta1 of the nascent polypeptide-associated complex (NAC)	Subunit beta1 of the nascent polypeptide-associated complex (NAC); involved in protein targeting, associated with cytoplasmic ribosomes; enhances DNA binding of the Gal4p activator; homolog of human BTF3b; EGD1 has a paralog, BTT1, that arose from the whole genome duplication
S000005959	YPL038W	MET31	METhionine requiring	480535	481068	1	Zinc-finger DNA-binding transcription factor	Zinc-finger DNA-binding transcription factor; targets strong transcriptional activator Met4p to promoters of sulfur metabolic genes; involved in transcriptional regulation of the methionine biosynthetic genes; feedforward loop controlling expression of MET32 and the lack of such a loop for MET31 may account for the differential actions of Met31p and Met32p; MET31 has a paralog, MET32, that arose from the whole genome duplication
S000005960	YPL039W	""	""	479225	480175	1	Putative protein of unknown function	Putative protein of unknown function; YPL039W is not an essential gene
S000005961	YPL040C	ISM1	Isoleucyl tRNA Synthetase of Mitochondria	475993	479001	-1	Mitochondrial isoleucyl-tRNA synthetase	Mitochondrial isoleucyl-tRNA synthetase; null mutant is deficient in respiratory growth; human homolog IARS2 implicated in mitochondrial diseases, can partially complement yeast null mutant
S000005962	YPL041C	MRX11	Mitochondrial oRganization of gene eXpression (MIOREX)	475118	475741	-1	Protein that associates with mitochondrial ribosome	Protein that associates with mitochondrial ribosome; SWAT-GFP and mCherry fusion proteins localize to the mitochondria; involved in maintenance of telomere length
S000005963	YPL042C	SSN3	Suppressor of SNf1	473040	474707	-1	Component of the RNA polymerase II mediator complex	Component of the RNA polymerase II mediator complex; cyclin-dependent protein kinase involved in phosphorylation of the mediator component Pgd1p and suppression of transcription activation by RNA polymerase II; involved in glucose repression
S000005964	YPL043W	NOP4	NucleOlar Protein	469939	471996	1	Nucleolar protein	Nucleolar protein; essential for processing and maturation of 27S pre-rRNA and large ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; contains four RNA recognition motifs (RRMs); human ortholog RBM28 implicated in ANE syndrome
S000005966	YPL045W	VPS16	Vacuolar Protein Sorting	467260	469656	1	Subunit of the HOPS and the CORVET complexes	Subunit of the HOPS and the CORVET complexes; part of the Class C Vps complex essential for membrane docking and fusion at Golgi-to-endosome and endosome-to-vacuole protein transport stages
S000005967	YPL046C	ELC1	ELongin C	466644	466943	-1	Elongin C, conserved among eukaryotes	Elongin C, conserved among eukaryotes; forms a complex with Cul3p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; plays a role in global genomic repair
S000005968	YPL047W	SGF11	SaGa associated Factor 11kDa	465962	466261	1	Integral subunit of SAGA histone acetyltransferase complex	Integral subunit of SAGA histone acetyltransferase complex; regulates transcription of a subset of SAGA-regulated genes, required for the Ubp8p association with SAGA and for H2B deubiquitylation
S000005969	YPL048W	CAM1	Calcium And Membrane-binding protein	464401	465648	1	One of two isoforms of the gamma subunit of eEF1B	One of two isoforms of the gamma subunit of eEF1B; stimulates the release of GDP from eEF1A (Tef1p/Tef2p) post association with the ribosomal complex with eEF1Balpha subunit; nuclear protein required for transcription of MXR1; binds the MXR1 promoter in the presence of other nuclear factors; binds calcium and phospholipids
S000005970	YPL049C	DIG1	Down-regulator of Invasive Growth	462481	463839	-1	MAP kinase-responsive inhibitor of the Ste12p transcription factor	MAP kinase-responsive inhibitor of the Ste12p transcription factor; involved in the regulation of mating-specific genes and the invasive growth pathway; related regulators Dig1p and Dig2p bind to Ste12p; DIG1 has a paralog, DIG2, that arose from the whole genome duplication
S000005971	YPL050C	MNN9	MaNNosyltransferase	460779	461966	-1	Subunit of Golgi mannosyltransferase complex	Subunit of Golgi mannosyltransferase complex; this complex mediates elongation of the polysaccharide mannan backbone; forms a separate complex with Van1p that is also involved in backbone elongation; this complex also contains Anp1p, Mnn10p, Mnn11p, and Hoc1p
S000005972	YPL051W	ARL3	ADP-Ribosylation factor-Like	459963	460559	1	ARF-like small GTPase of the RAS superfamily	ARF-like small GTPase of the RAS superfamily; required for recruitment of Arl1p, a GTPase that regulates membrane traffic, to the Golgi apparatus; NatC-catalyzed N-terminal acetylation regulates Golgi membrane association mediated by interaction with membrane receptor, Sys1p; similar to ADP-ribosylation factor and orthologous to mammalian ARFRP1
S000005973	YPL052W	OAZ1	Ornithine decarboxylase AntiZyme	458799	459678	1	Regulator of ornithine decarboxylase Spe1p	Regulator of ornithine decarboxylase Spe1p; antizyme that binds to Spe1p to stimulate ubiquitin-independent degradation by the proteasome; binding of polyamines to nascent Oaz1p during translation stimulates +1 ribosomal frameshifting, allowing translation of full-length Oaz1p
S000005974	YPL053C	KTR6	Kre Two Related	457118	458458	-1	Probable mannosylphosphate transferase	Probable mannosylphosphate transferase; involved in the synthesis of core oligosaccharides in protein glycosylation pathway; facilitates the exit of Mnn4p from the ER via COPII-coated vesicles; member of the KRE2/MNT1 mannosyltransferase family; KTR6 has a paralog, KRE2, that arose from the whole genome duplication
S000005975	YPL054W	LEE1	""	455759	456664	1	Zinc-finger protein of unknown function	Zinc-finger protein of unknown function
S000005976	YPL055C	LGE1	LarGE cells	453992	454990	-1	Protein involved in histone H2B ubiquitination	Protein involved in histone H2B ubiquitination; acts as cofactor for Bre1p; null mutant forms abnormally large cells, and homozygous diploid null mutant displays delayed premeiotic DNA synthesis and reduced efficiency of meiotic nuclear division
S000005977	YPL056C	LCL1	Long Chronological Lifespan	453430	453735	-1	Putative protein of unknown function	Putative protein of unknown function; deletion mutant is fluconazole resistant and has long chronological lifespan
S000005978	YPL057C	SUR1	SUppressor of Rvs161 and rvs167 mutations	451909	453057	-1	Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit	Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p; SUR1 has a paralog, CSH1, that arose from the whole genome duplication
S000005979	YPL058C	PDR12	Pleiotropic Drug Resistance	445842	450377	-1	Plasma membrane ATP-binding cassette (ABC) transporter	Plasma membrane ATP-binding cassette (ABC) transporter; weak-acid-inducible multidrug transporter required for weak organic acid resistance; induced by sorbate and benzoate and regulated by War1p; mutants exhibit sorbate hypersensitivity
S000005980	YPL059W	GRX5	GlutaRedoXin	444579	445031	1	Glutathione-dependent oxidoreductase	Glutathione-dependent oxidoreductase; mitochondrial matrix protein involved at an early step in the biogenesis of iron-sulfur centers along with Bol1p; hydroperoxide and superoxide-radical responsive; monothiol glutaredoxin subfamily member along with Grx3p and Grx4p
S000005981	YPL060W	MFM1	Mrs2 Function Modulating factor	434523	435764	1	Mitochondrial inner membrane magnesium transporter	Mitochondrial inner membrane magnesium transporter; involved in maintenance of mitochondrial magnesium concentrations and membrane potential; indirectly affects splicing of group II introns; functionally and structurally related to Mrs2p
S000005982	YPL061W	ALD6	ALdehyde Dehydrogenase	432588	434090	1	Cytosolic aldehyde dehydrogenase	Cytosolic aldehyde dehydrogenase; activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; localizes to the mitochondrial outer surface upon oxidative stress; negative regulator of nonselective autophagy; Stb5p directly regulates the expression of ALD6 mRNA and directly binds to the ALD6 promoter
S000005983	YPL062W	""	""	431895	432299	1	Putative protein of unknown function	Putative protein of unknown function; conserved across S. cerevisiae strains; deletion decreases transcription of downstream gene ALD6; YPL062W is not an essential gene; null mutant shows decrease in terpenoid production; homozygous diploid mutant shows a decrease in glycogen accumulation
S000005984	YPL063W	TIM50	Translocase of the Inner Mitochondrial membrane	429939	431369	1	Essential component of the TIM23 complex	Essential component of the TIM23 complex; acts as receptor for the translocase of the inner mitochondrial membrane (TIM23) complex guiding incoming precursors from the TOM complex; may control the gating of the Tim23p-Tim17p channel
S000005985	YPL064C	CWC27	Complexed With Cef1p	428711	429616	-1	Component of a complex containing Cef1p	Component of a complex containing Cef1p; putatively involved in pre-mRNA splicing; has similarity to S. pombe Cwf27p; protein abundance increases in response to DNA replication stress
S000005986	YPL065W	VPS28	Vacuolar Protein Sorting	427927	428655	1	Component of the ESCRT-I complex	Component of the ESCRT-I complex; complex is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p; other members include Stp22p, Srn2p, Vps28p, and Mvb12p
S000005987	YPL066W	RGL1	Rho1 GEF Localizing	426233	427672	1	Regulator of Rho1p signaling, cofactor of Tus1p	Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source
S000005988	YPL067C	HTC1	Histidine Triad with Channel	425251	425847	-1	Putative protein of unknown function	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YPL067C is not an essential gene
S000005989	YPL068C	""	""	424215	425096	-1	Protein of unknown function	Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and is induced in response to the DNA-damaging agent MMS
S000005990	YPL069C	BTS1	Bet Two Suppressor	422885	423892	-1	Geranylgeranyl diphosphate synthase (GGPPS)	Geranylgeranyl diphosphate synthase (GGPPS); increases the intracellular pool of geranylgeranyl diphosphate, suppressor of bet2 mutation that causes defective geranylgeranylation of small GTP-binding proteins that mediate vesicular traffic
S000005991	YPL070W	MUK1	coMpUtationally-linked to Kap95	420948	422786	1	Guanine nucleotide exchange factor (GEF)	Guanine nucleotide exchange factor (GEF); involved in vesicle-mediated vacuolar transport, including Golgi-endosome trafficking and sorting through the multivesicular body (MVB); specifically stimulates the intrinsic guanine nucleotide exchange activity of Rab family members (Vps21p/Ypt52p/Ypt53p); partially redundant with GEF VPS9; required for localization of the CORVET complex to endosomes; contains a VPS9 domain
S000005992	YPL071C	""	""	420048	420518	-1	Putative protein of unknown function	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
S000005993	YPL072W	UBP16	UBiquitin-specific Protease	418512	420011	1	Putative deubiquitinating enzyme anchored to the outer mitochondrial m	Putative deubiquitinating enzyme anchored to the outer mitochondrial membrane; probably not important for general mitochondrial functioning, but may perform a more specialized function at mitochondria
S000005994	YPL073C	""	""	418499	418984	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps with verified gene UBP16/YPL072W; may interact with ribosome based on co-purification experiments
S000005995	YPL074W	YTA6	Yeast Tat-binding Analog	415763	418027	1	Putative ATPase of the CDC48/PAS1/SEC18 (AAA) family	Putative ATPase of the CDC48/PAS1/SEC18 (AAA) family; localized to the cortex of mother cells but not to daughter cells; relocalizes from cytoplasm to plasma membrane foci upon DNA replication stress
S000005996	YPL075W	GCR1	GlyColysis Regulation	412254	415362	1	Transcriptional activator of genes involved in glycolysis	Transcriptional activator of genes involved in glycolysis; DNA-binding protein that interacts and functions with the transcriptional activator Gcr2p; contains a long intron, which allows multiple RNA and protein isoforms with varying expression levels depending on glucose availability
S000005997	YPL076W	GPI2	GlycosylPhosphatidylInositol anchor biosynthesis	410443	411285	1	Protein involved in the synthesis of GlcNAc-PI	Protein involved in the synthesis of GlcNAc-PI; GlcNAc-PI is the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; homologous to the human PIG-C protein; GlcNAc-PI stands for N-acetylglucosaminyl phosphatidylinositol
S000005998	YPL077C	""	""	409368	410090	-1	Putative protein of unknown function	Putative protein of unknown function; regulates PIS1 expression; mutant displays spore wall assembly defect in ether sensitivity screen; YPL077C is not an essential gene; YPL077C has a paralog, YBR197C, that arose from the whole genome duplication
S000005999	YPL078C	ATP4	ATP synthase	408010	408744	-1	Subunit b of the stator stalk of mitochondrial F1F0 ATP synthase	Subunit b of the stator stalk of mitochondrial F1F0 ATP synthase; ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; contributes to the oligomerization of the complex, which in turn determines the shape of inner membrane cristae; phosphorylated
S000006000	YPL079W	RPL21B	Ribosomal Protein of the Large subunit	406636	407539	1	Ribosomal 60S subunit protein L21B	Ribosomal 60S subunit protein L21B; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21B has a paralog, RPL21A, that arose from the whole genome duplication
S000006001	YPL080C	""	""	406172	406498	-1	Putative protein of unknown function	Putative protein of unknown function; conserved across S. cerevisiae strains
S000006002	YPL081W	RPS9A	Ribosomal Protein of the Small subunit	404950	406044	1	Protein component of the small (40S) ribosomal subunit	Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9A has a paralog, RPS9B, that arose from the whole genome duplication
S000006004	YPL083C	SEN54	Splicing ENdonuclease	396702	398105	-1	Subunit of the tRNA splicing endonuclease	Subunit of the tRNA splicing endonuclease; tRNA splicing endonuclease (Sen complex) is composed of Sen2p, Sen15p, Sen34p, and Sen54p; Sen complex also cleaves the CBP1 mRNA at the mitochondrial surface
S000006005	YPL084W	BRO1	BCK1-like Resistance to Osmotic shock	394038	396572	1	Cytoplasmic class E vacuolar protein sorting (VPS) factor	Cytoplasmic class E vacuolar protein sorting (VPS) factor; coordinates protein sorting and deubiquitination in the multivesicular body (MVB) pathway by recruiting Doa4p to endosomes
S000006006	YPL085W	SEC16	SECretory	387067	393654	1	COPII vesicle coat protein required for ER transport vesicle budding	COPII vesicle coat protein required for ER transport vesicle budding; essential factor in endoplasmic reticulum exit site (ERES) formation, as well as in COPII-mediated ER-to-Golgi traffic; bound to periphery of ER membranes and may act to stabilize initial COPII complexes; interacts with Sec23p, Sec24p and Sec31p
S000006007	YPL086C	ELP3	ELongator Protein	384773	386446	-1	Subunit of Elongator complex	Subunit of Elongator complex; Elongator is required for modification of wobble nucleosides in tRNA; exhibits histone acetyltransferase activity that is directed to histones H3 and H4; disruption confers resistance to K. lactis zymotoxin; human homolog ELP3 can partially complement yeast elp3 null mutant
S000006008	YPL087W	YDC1	Yeast DihydroCeramidase	383455	384408	1	Alkaline dihydroceramidase, involved in sphingolipid metabolism	Alkaline dihydroceramidase, involved in sphingolipid metabolism; preferentially hydrolyzes dihydroceramide to a free fatty acid and dihydrosphingosine; has a minor reverse activity; YDC1 has a paralog, YPC1, that arose from the whole genome duplication
S000006009	YPL088W	""	""	381965	382993	1	Putative aryl alcohol dehydrogenase	Putative aryl alcohol dehydrogenase; localizes to cytosol; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance
S000006010	YPL089C	RLM1	Resistance to Lethality of MKK1P386 overexpression	379120	381150	-1	MADS-box transcription factor	MADS-box transcription factor; component of the protein kinase C-mediated MAP kinase pathway involved in the maintenance of cell integrity; phosphorylated and activated by the MAP-kinase Slt2p; RLM1 has a paralog, SMP1, that arose from the whole genome duplication
S000006011	YPL090C	RPS6A	Ribosomal Protein of the Small subunit	377291	378395	-1	Protein component of the small (40S) ribosomal subunit	Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S6, no bacterial homolog; phosphorylated on S233 by Ypk3p in a TORC1-dependent manner, and on S232 in a TORC1/2-dependent manner by Ypk1/2/3p; RPS6A has a paralog, RPS6B, that arose from the whole genome duplication
S000006012	YPL091W	GLR1	GLutathione Reductase	375502	376953	1	Cytosolic and mitochondrial glutathione oxidoreductase	Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; cytosolic Glr1p is the main determinant of the glutathione redox state of the mitochondrial intermembrane space; mitochondrial Glr1p has a role in resistance to hyperoxia; also detected in peroxisomes; protein abundance increases in response to DNA replication stress
S000006013	YPL092W	SSU1	Sensitive to SUlfite	373793	375169	1	Plasma membrane sulfite pump involved in sulfite metabolism	Plasma membrane sulfite pump involved in sulfite metabolism; required for efficient sulfite efflux; major facilitator superfamily protein
S000006014	YPL093W	NOG1	NucleOlar G-protein	370978	372921	1	Putative GTPase	Putative GTPase; associates with free 60S ribosomal subunits in the nucleolus and is required for 60S ribosomal subunit biogenesis; constituent of 66S pre-ribosomal particles; member of the ODN family of nucleolar G-proteins
S000006015	YPL094C	SEC62	SECretory	369839	370663	-1	Essential subunit of Sec63 complex	Essential subunit of Sec63 complex; with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER; cotranslationally N-acetylated by NatA; other members are Sec63p, Sec66p, and Sec72p
S000006016	YPL095C	EEB1	Ethyl Ester Biosynthesis	368071	369441	-1	Acyl-coenzymeA:ethanol O-acyltransferase	Acyl-coenzymeA:ethanol O-acyltransferase; responsible for the major part of medium-chain fatty acid ethyl ester biosynthesis during fermentation; possesses short-chain esterase activity; may be involved in lipid metabolism and detoxification; EEB1 has a paralog, EHT1, that arose from the whole genome duplication
S000006017	YPL096W	PNG1	Peptide N-Glycanase	366927	368018	1	Conserved peptide N-glycanase	Conserved peptide N-glycanase; deglycosylating enzyme that cleaves N-glycans that are attached to misfolded ERAD substrate glycoproteins prior to proteasome-dependent degradation; localizes to the cytoplasm and nucleus; activity is enhanced by interaction with Rad23p; human ortholog NGLY1 is associated with a syndrome characterized by developmental delays, epilepsy, absence of tears and liver disease
S000006018	YPL097W	MSY1	Mitochondrial aminoacyl-tRNA Synthetase, tyrosine (Y)	364952	366430	1	Mitochondrial tyrosyl-tRNA synthetase	Mitochondrial tyrosyl-tRNA synthetase
S000006019	YPL098C	MGR2	Mitochondrial Genome Required	364386	364727	-1	Core component of the TIM23 protein translocase complex	Core component of the TIM23 protein translocase complex; couples Tim21 with the core Tim23 translocase; provides quality control for membrane protein insertion, regulating their release into the inner membrane by the TIM23 complex; required for mitochondrial import of presequence-containing proteins at elevated temperature; forms the translocation path with Tim17p, sealing the lateral opening of the Tim17p cavity; required for viability of cells lacking the mitochondrial genome (petite-negative)
S000006020	YPL099C	INA17	INner membrane Assembly 17 kDa	363520	364068	-1	F1F0 ATPase synthase peripheral stalk assembly factor	F1F0 ATPase synthase peripheral stalk assembly factor; subunit of the matrix-exposed inner mitochondrial membrane localized INA complex (Ina22p-Ina17p) involved in assembly of the F1F0 peripheral stalk; co-purifies with Ina22p and ATP synthase subunits; null mutant displays elevated frequency of mitochondrial genome loss and has a respiratory growth defect
S000006021	YPL100W	ATG21	AuTophaGy related	361872	363362	1	Phosphoinositide binding protein	Phosphoinositide binding protein; required for vesicle formation in cytoplasm-to-vacuole targeting (CVT) pathway, autophagy, micronucleophagy and mitophagy; binds to several phosphoinositides including PtdIns(3,5)P2, PI3P and PI4P; involved in PI3P-dependent recruitment and organization of both Atg12-Atg5-Atg16 complex and Atg8p at pre-autophagosomal structure; necessary for oxidant-induced cell death; targeted to vacuole via AP-3 pathway; WD-40 repeat containing PROPPIN family member
S000006022	YPL101W	ELP4	ELongator Protein	360209	361579	1	Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex	Subunit of hexameric RecA-like ATPase Elp456 Elongator subcomplex; which is required for modification of wobble nucleosides in tRNA; required for Elongator structural integrity; null mutation is functionally complemented by human ELP4
S000006023	YPL102C	""	""	360003	360305	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; deletion mutation enhances replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene ELP4
S000006024	YPL103C	FMP30	Found in Mitochondrial Proteome	358000	359406	-1	Protein with a role in maintaining mitochondrial morphology	Protein with a role in maintaining mitochondrial morphology; also involved in maintaining normal cardiolipin levels; mitochondrial inner membrane protein; proposed to be involved in N-acylethanolamine metabolism; related to mammalian N-acylPE-specific phospholipase D
S000006025	YPL104W	MSD1	Mitochondrial aminoacyl-tRNA Synthetase, Aspartate (D)	355700	357676	1	Mitochondrial aspartyl-tRNA synthetase	Mitochondrial aspartyl-tRNA synthetase; required for acylation of aspartyl-tRNA; yeast and bacterial aspartyl-, asparaginyl-, and lysyl-tRNA synthetases contain regions with high sequence similarity, suggesting a common ancestral gene
S000006026	YPL105C	SYH1	SmY2 Homolog	352863	355412	-1	Protein of unknown function that influences nuclear pore distribution	Protein of unknown function that influences nuclear pore distribution; co-purifies with ribosomes; contains a GYF domain, which bind proline-rich sequences; deletion extends chronological lifespan; SYH1 has a paralog, SMY2, that arose from the whole genome duplication
S000006027	YPL106C	SSE1	Stress Seventy subfamily E	350194	352275	-1	ATPase component of heat shock protein Hsp90 chaperone complex	ATPase component of heat shock protein Hsp90 chaperone complex; serves as nucleotide exchange factor to load ATP onto the SSA class of cytosolic Hsp70s; plays a role in prion propagation and determining prion variants; binds unfolded proteins; member of Hsp110 subclass of HSP70 proteins; deletion results in spindle elongation in S phase; SSE1 has a paralog, SSE2, that arose from the whole genome duplication
S000006028	YPL107W	DPC25	Delta-Psi dependent mitochondrial import and Cleavage protein of ~25 kDa	349119	349865	1	Putative mitochondrial protein of unknown function	Putative mitochondrial protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; DPC25 is not an essential gene
S000006029	YPL108W	""	""	348446	348952	1	Cytoplasmic protein of unknown function	Cytoplasmic protein of unknown function; non-essential gene that is induced in a GDH1 deleted strain with altered redox metabolism; GFP-fusion protein is induced in response to the DNA-damaging agent MMS
S000006030	YPL109C	CQD1	CoQ Distribution	345264	347389	-1	UbiB protein kinase-like family member	UbiB protein kinase-like family member; mitochondrial inner membrane protein that interacts with outer membrane proteins Por1p and Om14p to form a MICOS-independent mitochondrial contact site; role in mitochondrial organization; contributes to mitochondrial lipid homeostasis; controls the cellular distribution of coenzyme Q (CoQ); contains a potential mitochondrial targeting sequence and predicted transmembrane domain
S000006031	YPL110C	GDE1	GlycerophosphoDiEsterase	341068	344739	-1	Glycerophosphocholine (GroPCho) phosphodiesterase	Glycerophosphocholine (GroPCho) phosphodiesterase; hydrolyzes GroPCho to choline and glycerolphosphate, for use as a phosphate source and as a precursor for phosphocholine synthesis; may interact with ribosomes
S000006032	YPL111W	CAR1	Catabolism of ARginine	339944	340945	1	Arginase, catabolizes arginine to ornithine and urea	Arginase, catabolizes arginine to ornithine and urea; expression responds to both induction by arginine and nitrogen catabolite repression; disruption decreases production of carcinogen ethyl carbamate during wine fermentation and also enhances freeze tolerance
S000006033	YPL112C	PEX25	PEroXisome related	337436	338620	-1	Peripheral peroxisomal membrane peroxin	Peripheral peroxisomal membrane peroxin; required for the regulation of peroxisome size and maintenance, recruits GTPase Rho1p to peroxisomes, induced by oleate, interacts with Pex27p; PEX25 has a paralog, PEX27, that arose from the whole genome duplication
S000006034	YPL113C	""	""	335953	337143	-1	Glyoxylate reductase	Glyoxylate reductase; acts on glyoxylate and hydroxypyruvate substrates; YPL113C is not an essential gene
S000006035	YPL114W	""	""	335948	336367	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; largely overlaps ORF YPL113C; diploid deletion in BY4743 strain background exhibits high budding index
S000006036	YPL115C	BEM3	Bud EMergence	332100	335486	-1	Rho GTPase activating protein (RhoGAP)	Rho GTPase activating protein (RhoGAP); involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly
S000006037	YPL116W	HOS3	Hda One Similar	329606	331699	1	Trichostatin A-insensitive homodimeric histone deacetylase (HDAC)	Trichostatin A-insensitive homodimeric histone deacetylase (HDAC); specificity in vitro for histones H3, H4, H2A, and H2B; similar to Hda1p, Rpd3p, Hos1p, and Hos2p; deletion results in increased histone acetylation at rDNA repeats
S000006038	YPL117C	IDI1	Isopentenyl Diphosphate Isomerase	327864	328730	-1	Isopentenyl diphosphate:dimethylallyl diphosphate isomerase	Isopentenyl diphosphate:dimethylallyl diphosphate isomerase; catalyzes an essential activation step in the isoprenoid biosynthetic pathway; required for viability; isopentenyl diphosphate:dimethylallyl diphosphate isomerase is also known as IPP isomerase
S000006039	YPL118W	MRP51	Mitochondrial Ribosomal Protein	326628	327662	1	Mitochondrial ribosomal protein of the small subunit	Mitochondrial ribosomal protein of the small subunit; MRP51 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences
S000006040	YPL119C	DBP1	Dead Box Protein	324411	326264	-1	ATP-dependent RNA helicase of the DEAD-box protein family	ATP-dependent RNA helicase of the DEAD-box protein family; accelerates eIF4F-dependent assembly of 48S translation preinitiation complex (PIC), stimulating recruitment of mRNAs with long, structured 5'-UTRs; cooperates with DEAD-box RNA helicase and paralog Ded1p, stimulating PIC attachment and highly processive scanning by the 40S ribosomal subunit; protein abundance increases in response to DNA replication stress
S000006041	YPL120W	VPS30	Vacuolar Protein Sorting	322071	323744	1	Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II	Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy, Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; C-terminus has novel globular fold essential for autophagy through the targeting of the PI3-kinase complex I to the pre-autophagosomal structure; ortholog of higher eukaryote gene Beclin 1; human BECN1 can complement yeast null mutant
S000006042	YPL121C	MEI5	MEIosis	320962	321630	-1	Meiosis-specific protein involved in meiotic recombination	Meiosis-specific protein involved in meiotic recombination; involved in DMC1-dependent meiotic recombination; forms heterodimer with Sae3p; proposed to be an assembly factor for Dmc1p
S000006043	YPL122C	TFB2	Transcription Factor B	319226	320767	-1	Subunit of TFIIH and nucleotide excision repair factor 3 complexes	Subunit of TFIIH and nucleotide excision repair factor 3 complexes; involved in transcription initiation, required for nucleotide excision repair, similar to 52 kDa subunit of human TFIIH
S000006044	YPL123C	RNY1	RiboNuclease from Yeast	317641	318945	-1	Vacuolar RNase of the T(2) family	Vacuolar RNase of the T(2) family; relocalizes to the cytosol where it cleaves tRNAs upon oxidative or stationary phase stress; required for tRNA-specific translational pausing during oxidative stress; promotes apoptosis under stress conditions and this function is independent of Rny1p catalytic activity
S000006045	YPL124W	SPC29	Spindle Pole Component	316755	317516	1	Inner plaque spindle pole body (SPB) component	Inner plaque spindle pole body (SPB) component; links the central plaque component Spc42p to the inner plaque component Spc110p; required for SPB duplication
S000006046	YPL125W	KAP120	KAryoPherin	313388	316486	1	Karyopherin responsible for the nuclear import of Rpf1p	Karyopherin responsible for the nuclear import of Rpf1p; Rpf1p is a ribosome maturation factor
S000006048	YPL127C	HHO1	Histone H One	308828	309604	-1	Histone H1, linker histone with roles in meiosis and sporulation	Histone H1, linker histone with roles in meiosis and sporulation; decreasing levels early in sporulation may promote meiosis, and increasing levels during sporulation facilitate compaction of spore chromatin; binds to promoters and within genes in mature spores; may be recruited by Ume6p to promoter regions, contributing to transcriptional repression outside of meiosis; suppresses DNA repair involving homologous recombination
S000006049	YPL128C	TBF1	TTAGGG repeat-Binding Factor	306533	308221	-1	Telobox-containing general regulatory factor	Telobox-containing general regulatory factor; binds TTAGGG repeats within subtelomeric anti-silencing regions (STARs), blocking silent chromatin propagation; binds majority of snoRNA gene promoters, required for full snoRNA expression; caps DSB flanked by long T2AG3 repeats and blocks checkpoint activation; involved in nucleosome displacement during double-strand break repair
S000006050	YPL129W	TAF14	TATA binding protein-Associated Factor	305298	306137	1	Subunit of TFIID, TFIIF, INO80, SWI/SNF, and NuA3 complexes	Subunit of TFIID, TFIIF, INO80, SWI/SNF, and NuA3 complexes; involved in RNA polymerase II transcription initiation and in chromatin modification; serves as an additional DNA binding domain for the complex; contains a YEATS domain
S000006051	YPL130W	SPO19	SPOrulation	304387	305058	1	Meiosis-specific prospore protein	Meiosis-specific prospore protein; required to produce bending force necessary for proper assembly of the prospore membrane during sporulation; identified as a weak high-copy suppressor of the spo1-1 ts mutation; SPO19 has a paralog, YOR214C, that arose from the whole genome duplication
S000006053	YPL132W	COX11	Cytochrome c OXidase	301716	302618	1	Protein required for delivery of copper to Cox1p	Protein required for delivery of copper to Cox1p; mitochondrial inner membrane protein; involved in oxidative stress response; association with mitochondrial ribosomes suggests that copper delivery may occur during translation of Cox1p
S000006054	YPL133C	RDS2	Regulator of Drug Sensitivity	299888	301228	-1	Transcription factor involved in regulating gluconeogenesis	Transcription factor involved in regulating gluconeogenesis; also involved in the regulation of glyoxylate cycle genes; member of the zinc cluster family of proteins; confers resistance to ketoconazole
S000006055	YPL134C	ODC1	OxoDicarboxylate Carrier	298571	299503	-1	Mitochondrial inner membrane transporter	Mitochondrial inner membrane transporter; 2-oxodicarboxylate transporter, exports 2-oxoadipate and 2-oxoglutarate from the mitochondrial matrix to the cytosol for lysine and glutamate biosynthesis and lysine catabolism; suppresses, in multicopy, an fmc1 null mutation; ODC1 has a paralog, ODC2, that arose from the whole genome duplication
S000006056	YPL135W	ISU1	IScU homolog	297553	298050	1	Conserved protein of the mitochondrial matrix	Conserved protein of the mitochondrial matrix; performs a scaffolding function during assembly of iron-sulfur clusters, interacts physically and functionally with yeast frataxin (Yfh1p); ISU1 has a paralog, ISU2, that arose from the whole genome duplication; isu1 isu2 double mutant is inviable; human homolog ISCU implicated in mitochondrial myopathy, can complement isu1 isu2 double mutant
S000006057	YPL136W	""	""	296286	296654	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the verified gene GIP3/YPL137C
S000006058	YPL137C	GIP3	Glc7 Interacting Protein	292817	296647	-1	Cytoplasmic protein that regulates protein phosphatase 1 Glc7p	Cytoplasmic protein that regulates protein phosphatase 1 Glc7p; overexpression relocalizes Glc7p from the nucleus and prevents chromosome segregation; may interact with ribosomes, based on co-purification experiments; GIP3 has a paralog, HER1, that arose from the whole genome duplication
S000006059	YPL138C	SPP1	Set1c, Phd finger Protein	291366	292427	-1	Subunit of COMPASS (Set1C)	Subunit of COMPASS (Set1C); a complex which methylates histone H3 on lysine 4 and is required in telomeric transcriptional silencing; promotes meiotic DSB formation by interacting with H3K4me3 and Rec107p, a protein required for Spo11p-catalyzed DSB formation located on chromosome axes; interacts with Orc2p; PHD finger domain protein similar to human CGBP, an unmethylated CpG binding protein; relocalizes to cytosol in response to hypoxia
S000006060	YPL139C	UME1	Unscheduled Meiotic gene Expression	289669	291051	-1	Component of both the Rpd3S and Rpd3L histone deacetylase complexes	Component of both the Rpd3S and Rpd3L histone deacetylase complexes; negative regulator of meiosis; required for repression of a subset of meiotic genes during vegetative growth, binding of histone deacetylase Rpd3p required for activity, contains a NEE box and a WD repeat motif; homologous with Wtm1p; UME1 has a paralog, WTM2, that arose from the whole genome duplication
S000006061	YPL140C	MKK2	Mitogen-activated Kinase Kinase	287514	289034	-1	MAPKK involved in the protein kinase C signaling pathway	MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk1p; MKK2 has a paralog, MKK1, that arose from the whole genome duplication
S000006062	YPL141C	FRK1	Fatty acyl-CoA synthetase and RNA processing-associated Kinase	283464	286061	-1	Protein kinase	Protein kinase; minor role relative to Kin4p in regulating peroxisomal transport and vacuolar inheritance; protects the peroxisomal Myo2p receptor Inp2p from degradation in mother cells along with Kin4p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; interacts with rRNA transcription and ribosome biogenesis factors and the long chain fatty acyl-CoA synthetase Faa3p; FRK1 has a paralog, KIN4, that arose from the whole genome duplication
S000006064	YPL143W	RPL33A	Ribosomal Protein of the Large subunit	282122	282970	1	Ribosomal 60S subunit protein L33A	Ribosomal 60S subunit protein L33A; N-terminally acetylated; rpl33a null mutant exhibits slow growth while rpl33a rpl33b double null mutant is inviable; homologous to mammalian ribosomal protein L35A, no bacterial homolog; RPL33A has a paralog, RPL33B, that arose from the whole genome duplication
S000006065	YPL144W	POC4	PrOteasome Chaperone	280480	280926	1	Component of a heterodimeric Poc4p-Irc25p chaperone	Component of a heterodimeric Poc4p-Irc25p chaperone; involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions; upregulates proteasome assembly in response to the unfolded protein response activated by mistargeting of proteins (UPRam)
S000006066	YPL145C	KES1	KrE11-1 Suppressor	278395	279699	-1	Sterol/phosphatidylinositol-4-phosphate (PI(4)P) exchanger	Sterol/phosphatidylinositol-4-phosphate (PI(4)P) exchanger; one of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication
S000006067	YPL146C	NOP53	NucleOlar Protein	276162	277529	-1	Nucleolar protein	Nucleolar protein; involved in biogenesis of the 60S subunit of the ribosome; interacts with rRNA processing factors Cbf5p and Nop2p and with the nucleolar proteins Nop17p and Nip7p; null mutant is viable but growth is severely impaired
S000006068	YPL147W	PXA1	PeroXisomal ABC-transporter	273255	275867	1	Subunit of heterodimeric peroxisomal ABC transport complex, with Pxa2p	Subunit of heterodimeric peroxisomal ABC transport complex, with Pxa2p; required for import of long-chain fatty acids into peroxisomes; similar to human adrenoleukodystrophy transporters ABCD1and ABCD2, and ALD-related proteins; mutations in ABCD1 cause X-linked adrenoleukodystrophy (X-ALD), a peroxisomal disorder; human ABCD1 and ABCD2 can each partially complement yeast pxa1 pxa2 double null mutant
S000006069	YPL148C	PPT2	Phosphopantetheine:Protein Transferase	272294	272815	-1	Phosphopantetheine:protein transferase (PPTase)	Phosphopantetheine:protein transferase (PPTase); activates mitochondrial acyl carrier protein (Acp1p) by phosphopantetheinylation
S000006070	YPL149W	ATG5	AuTophaGy related	271310	272194	1	Conserved protein involved in autophagy and the Cvt pathway	Conserved protein involved in autophagy and the Cvt pathway; undergoes conjugation with Atg12p to form a complex involved in Atg8p lipidation; Atg5p-Atg12p conjugate enhances E2 activity of Atg3 by rearranging its catalytic site, also forms a complex with Atg16p; the Atg5-Atg12/Atg16 complex binds to membranes and is essential for autophagosome formation; also involved in methionine restriction extension of chronological lifespan in an autophagy-dependent manner
S000006071	YPL150W	""	""	268188	270893	1	Protein kinase of unknown cellular role	Protein kinase of unknown cellular role; binds phosphatidylinositols and cardiolipin in a large-scale study
S000006072	YPL151C	PRP46	Pre-mRNA Processing	266180	267535	-1	Member of the NineTeen Complex (NTC)	Member of the NineTeen Complex (NTC); this complex contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs
S000006073	YPL152W	RRD2	Resistant to Rapamycin Deletion	265028	266104	1	Peptidyl-prolyl cis/trans-isomerase	Peptidyl-prolyl cis/trans-isomerase; also activates the phosphotyrosyl phosphatase activity of protein phosphatase 2A (PP2A); regulates G1 phase progression, the osmoresponse, microtubule dynamics; subunit of the Tap42p-Pph21p-Rrd2p complex; protein abundance increases in response to DNA replication stress
S000006075	YPL154C	PEP4	carboxyPEPtidase Y-deficient	259714	260931	-1	Vacuolar aspartyl protease (proteinase A)	Vacuolar aspartyl protease (proteinase A); required for post-translational precursor maturation of vacuolar proteinases; important for protein turnover after oxidative damage; plays a protective role in acetic acid induced apoptosis; synthesized as a zymogen, self-activates; targeted to vacuole via Vps10p-dependent endosomal vacuolar protein sorting pathway
S000006076	YPL155C	KIP2	KInesin related Protein	257216	259336	-1	Kinesin-related motor protein and plus-end directed microtubule (MT) p	Kinesin-related motor protein and plus-end directed microtubule (MT) polymerase; involved in mitotic spindle positioning, nuclear migration, microtubule polymerization and inhibition of MT catastrophe; stabilizes microtubules by targeting Bik1p to the MT plus end; Kip2p levels are controlled during the cell cycle
S000006077	YPL156C	PRM4	Pheromone-Regulated Membrane protein	255913	256767	-1	Pheromone-regulated protein proposed to be involved in mating	Pheromone-regulated protein proposed to be involved in mating; predicted to have 1 transmembrane segment; transcriptionally regulated by Ste12p during mating and by Cat8p during the diauxic shift
S000006078	YPL157W	TGS1	TrimethylGuanosine Synthase	254814	255761	1	Trimethyl guanosine synthase, conserved nucleolar methyl transferase	Trimethyl guanosine synthase, conserved nucleolar methyl transferase; converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs; interacts with Swm2p, which may confer substrate specificity on Tgs1p
S000006079	YPL158C	AIM44	Altered Inheritance rate of Mitochondria	252034	254310	-1	Regulator of Cdc42p and Rho1p	Regulator of Cdc42p and Rho1p; regulates AMR closure through Hof1p; inhibits Cdc42-dependent Cla4 activation at the division site, to prevent budding in the old bud neck; recruits Nis1p and Nba1p to the division site with Nap1 and the Rax1p-Rax2p dependent inheritance of Nis1p and Nba1p to bud scars to prevent division site repolarization; keeps Rho1p at the division site after AMR contraction to control secondary septum formation; relocalizes from bud neck to cytoplasm upon replication stress
S000006080	YPL159C	PET20	PETite colonies	250907	251668	-1	Mitochondrial protein	Mitochondrial protein; required for respiratory growth under some conditions and for stability of the mitochondrial genome; subunit of a complex containing Mrx6p, Pim1p, and Mam33p that may regulate mtDNA replication
S000006081	YPL160W	CDC60	Cell Division Cycle	246990	250262	1	Cytosolic leucyl tRNA synthetase	Cytosolic leucyl tRNA synthetase; ligates leucine to the appropriate tRNA; human homolog LARS can complement yeast temperature-sensitive mutant at restrictive temperature
S000006082	YPL161C	BEM4	Bud EMergence	244319	246220	-1	Protein involved in establishment of cell polarity and bud emergence	Protein involved in establishment of cell polarity and bud emergence; regulates filamentous-growth mitogen-activated protein kinase (MAPK) pathway; interacts with the Rho1p small GTP-binding protein, the pleckstrin homology (PH) domain of Cdc24p, and with the Rho-type GTPase Cdc42p; stabilizes Cdc42p levels, resulting in elevated fMAPK pathway signaling; involved in maintenance of proper telomere length
S000006083	YPL162C	""	""	243206	244027	-1	Putative protein of unknown function	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the membrane of vacuole with cell cycle-correlated morphology
S000006084	YPL163C	SVS1	Suppressor of Vanadate Sensitivity	241918	242700	-1	Cell wall and vacuolar protein	Cell wall and vacuolar protein; required for wild-type resistance to vanadate; SVS1 has a paralog, SRL1, that arose from the whole genome duplication
S000006085	YPL164C	MLH3	MutL Homolog	239350	241497	-1	Protein involved in DNA mismatch repair and meiotic recombination	Protein involved in DNA mismatch repair and meiotic recombination; involved in crossing-over during meiotic recombination; forms a complex with Mlh1p; mammalian homolog is implicated mammalian microsatellite instability
S000006086	YPL165C	SET6	SET domain-containing	237956	239077	-1	SET domain protein of unknown function	SET domain protein of unknown function; deletion heterozygote is sensitive to compounds that target ergosterol biosynthesis, may be involved in compound availability
S000006087	YPL166W	ATG29	AuTophaGy related	237338	237979	1	Autophagy-specific protein	Autophagy-specific protein; required for recruiting other ATG proteins to the pre-autophagosomal structure (PAS); interacts with Atg17p and localizas to the PAS in a manner interdependent with Atg17p and Cis1p; not conserved; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
S000006088	YPL167C	REV3	REVersionless	232593	237107	-1	Catalytic subunit of DNA polymerase zeta (pol zeta)	Catalytic subunit of DNA polymerase zeta (pol zeta); involved in translesion synthesis during post-replication repair; required for mutagenesis induced by DNA damage; involved in double-strand break repair; may be involved in meiosis; forms a complex with Rev7p, Pol31p and Pol32p
S000006089	YPL168W	MRX4	Mitochondrial oRganization of gene eXpression (MIOREX)	231217	232509	1	Protein that associates with mitochondrial ribosome	Protein that associates with mitochondrial ribosome; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; expression may be cell cycle-regulated
S000006090	YPL169C	MEX67	Messenger RNA EXport factor of 67 kDa	229039	230838	-1	Poly(A)RNA binding protein involved in nuclear mRNA export	Poly(A)RNA binding protein involved in nuclear mRNA export; component of the nuclear pore and nuclear RNA export factor; ubiquitin-associated (UBA) domain interacts with Mip6p; ortholog of human TAP
S000006091	YPL170W	DAP1	DAmage resistance Protein	228314	228772	1	Heme-binding protein	Heme-binding protein; involved in regulation of cytochrome P450 protein Erg11p; damage response protein, related to mammalian membrane progesterone receptors; mutations lead to defects in telomeres, mitochondria, and sterol synthesis
S000006092	YPL171C	OYE3	Old Yellow Enzyme	226169	227371	-1	Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN)	Conserved NADPH oxidoreductase containing flavin mononucleotide (FMN); homologous to Oye2p with different ligand binding and catalytic properties; has potential roles in oxidative stress response and programmed cell death
S000006093	YPL172C	COX10	Cytochrome c OXidase	224353	225741	-1	Heme A:farnesyltransferase	Heme A:farnesyltransferase; catalyzes first step in conversion of protoheme to heme A prosthetic group required for cytochrome c oxidase activity; human ortholog COX10 can complement yeast cox10 null mutant; human ortholog COX10 is associated with mitochondrial disorders
S000006094	YPL173W	MRPL40	Mitochondrial Ribosomal Protein, Large subunit	223143	224036	1	Mitochondrial ribosomal protein of the large subunit	Mitochondrial ribosomal protein of the large subunit
S000006095	YPL174C	NIP100	Nuclear ImPort	220167	222773	-1	Large subunit of the dynactin complex	Large subunit of the dynactin complex; dynactin is involved in partitioning the mitotic spindle between mother and daughter cells; putative ortholog of mammalian p150(glued)
S000006096	YPL175W	SPT14	SuPpressor of Ty	218630	220088	1	UDP-glycosyltransferase subunit of the GPI-GnT complex	UDP-glycosyltransferase subunit of the GPI-GnT complex; UDP-GlcNAc-binding and catalytic subunit of the enzyme that mediates the first step in glycosylphosphatidylinositol (GPI) biosynthesis, mutations cause defects in transcription and in biogenesis of cell wall proteins
S000006097	YPL176C	TRE1	Transferrin REceptor like	216012	218363	-1	Transferrin receptor-like protein	Transferrin receptor-like protein; plasma membrane protein that binds Bsd2p and regulates ubiquitination and vacuolar degradation of the metal transporter Smf1p; functionally redundant with Tre2p; TRE1 has a paralog, TRE2, that arose from the whole genome duplication
S000006098	YPL177C	CUP9	""	213042	213962	-1	Homeodomain-containing transcriptional repressor	Homeodomain-containing transcriptional repressor; regulates expression of PTR2, which encodes a major peptide transporter; imported peptides activate ubiquitin-dependent proteolysis, resulting in degradation of Cup9p and de-repression of PTR2 transcription; CUP9 has a paralog, TOS8, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
S000006099	YPL178W	CBC2	Cap Binding Complex	212158	212784	1	Small subunit of the heterodimeric cap binding complex with Sto1p	Small subunit of the heterodimeric cap binding complex with Sto1p; interacts with Npl3p, possibly to package mRNA for export from the nucleus; may have a role in telomere maintenance; contains an RNA-binding motif
S000006100	YPL179W	PPQ1	Protein Phosphatase Q	208157	209806	1	Protein phosphatase that regulates the mating response	Protein phosphatase that regulates the mating response; negatively regulates the MAP kinase signaling cascade during mating; member of the serine/threonine phosphatase PP1 family
S000006101	YPL180W	TCO89	Tor Complex One	205248	207647	1	Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p)	Subunit of TORC1 (Tor1p or Tor2p-Kog1p-Lst8p-Tco89p); regulates global H3K56ac; TORC1 complex regulates growth in response to nutrient availability; cooperates with Ssd1p in the maintenance of cellular integrity; deletion strains are hypersensitive to rapamycin
S000006102	YPL181W	CTI6	Cyc8-Tup1 Interacting protein	203421	204941	1	Component of the Rpd3L histone deacetylase complex	Component of the Rpd3L histone deacetylase complex; relieves transcriptional repression by binding to the Cyc8p-Tup1p corepressor and recruiting the SAGA complex to the repressed promoter; contains a PHD finger domain
S000006103	YPL182C	""	""	203306	203689	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CTI6/YPL181W
S000006104	YPL183C	RTT10	Regulator of Ty1 Transposition	199495	202536	-1	WD40 domain-containing protein involved in endosomal recycling	WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rrt2p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; interacts with Trm7p for 2'-O-methylation of N34 of substrate tRNAs; has a role in regulation of Ty1 transposition; human ortholog is WDR6
S000006105	YPL184C	MRN1	Multicopy supressor of rsc nhp6	195950	197788	-1	mRNA-binding protein	mRNA-binding protein; binds to the 3'UTR of cell wall-related mRNAs and destabilizes these mRNAs during stress, acting antagonistically to Nab6p; links cell wall homeostasis to mitochondrial structure and function; binds specific categories of mRNAs, including those that contain upstream open reading frames (uORFs) and internal ribosome entry sites (IRES); interacts genetically with chromatin remodelers and splicing factors, linking chromatin state, splicing and as a result mRNA maturation
S000006106	YPL185W	""	""	195253	195648	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene UIP4/YPL186C
S000006107	YPL186C	UIP4	Ulp1 Interacting Protein	194512	195426	-1	Protein required for nuclear envelope integrity	Protein required for nuclear envelope integrity; involved in distribution of nuclear pore complexes; interacts with Ulp1p, a Ubl (ubiquitin-like protein)-specific protease for Smt3p protein conjugates; detected in a phosphorylated state in the mitochondrial outer membrane; also detected in ER and nuclear envelope
S000006108	YPL187W	MF(ALPHA)1	Mating Factor ALPHA	193648	194145	1	Alpha-factor mating pheromone made by alpha cells	Alpha-factor mating pheromone made by alpha cells; interacts with mating type a cells to induce cell cycle arrest and other responses leading to mating; also encoded by MF(ALPHA)2, although MF(ALPHA)1 produces most alpha-factor; binds copper(II) ions
S000006109	YPL188W	POS5	PerOxide Sensitive	191406	192650	1	Mitochondrial NADH kinase	Mitochondrial NADH kinase; phosphorylates NADH; also phosphorylates NAD(+) with lower specificity; required for the response to oxidative stress
S000006110	YPL189W	GUP2	Glycerol UPtake	189154	190983	1	Probable membrane protein	Probable membrane protein; possible role in proton symport of glycerol; member of the MBOAT family of putative membrane-bound O-acyltransferases; homolog of the mammalian Hedgehog pathway modulator HHAT; GUP2 has a paralog, GUP1, that arose from the whole genome duplication
S000006112	YPL191C	MIY2	MINDY deubiquitinase in yeast	183597	184679	-1	K48-specific deubiquitinating (DUB) enzyme	K48-specific deubiquitinating (DUB) enzyme; MINDY family endo-type deubiquitinase that preferentially cleaves long K48-linked polyubiquitin chains between moieties; diploid deletion strain exhibits high budding index; GFP-fusion protein localizes to the cytoplasm endoplasmic reticulum and cell periphery in high-throughput studies; YPL191C has a paralog, YGL082W, that arose from the whole genome duplication; ortholog of human MINDY2/FAM63B
S000006113	YPL192C	PRM3	Pheromone-Regulated Membrane protein	182655	183056	-1	Protein required for nuclear envelope fusion during karyogamy	Protein required for nuclear envelope fusion during karyogamy; pheromone-regulated; peripheral protein of the nuclear membrane; interacts with Kar5p at the spindle pole body
S000006114	YPL193W	RSA1	RiboSome Assembly	181403	182548	1	Protein involved in the assembly of 60S ribosomal subunits	Protein involved in the assembly of 60S ribosomal subunits; functionally interacts with Dbp6p; functions in a late nucleoplasmic step of the assembly
S000006115	YPL194W	DDC1	DNA Damage Checkpoint	179277	181115	1	DNA damage checkpoint protein	DNA damage checkpoint protein; part of a PCNA-like complex required for DNA damage response, required for pachytene checkpoint to inhibit cell cycle in response to unrepaired recombination intermediates; potential Cdc28p substrate; forms nuclear foci upon DNA replication stress
S000006116	YPL195W	APL5	clathrin Adaptor Protein complex Large chain	176223	179021	1	Delta adaptin-like subunit of the clathrin associated protein complex	Delta adaptin-like subunit of the clathrin associated protein complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; suppressor of loss of casein kinase 1 function; the clathrin associated protein complex is also known as AP-3
S000006117	YPL196W	OXR1	OXidation Resistance	175043	175864	1	Protein involved in regulation of vacuolar V-ATPase	Protein involved in regulation of vacuolar V-ATPase; inhibits V1-ATPase activity and triggers disassembly of V-ATPase holoenzyme; required for normal levels of resistance to oxidative damage; null mutants are sensitive to hydrogen peroxide; member of a conserved family of proteins found in eukaryotes
S000006118	YPL197C	""	""	174343	174756	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the ribosomal gene RPL7B
S000006119	YPL198W	RPL7B	Ribosomal Protein of the Large subunit	173152	174702	1	Ribosomal 60S subunit protein L7B	Ribosomal 60S subunit protein L7B; required for processing of 27SA3 pre-rRNA to 27SB pre-rRNA during assembly of large ribosomal subunit; depletion leads to a turnover of pre-rRNA; contains a conserved C-terminal Nucleic acid Binding Domain (NDB2); binds to Domain II of 25S and 5.8S rRNAs; homologous to mammalian ribosomal protein L7 and bacterial L30; RPL7B has a paralog, RPL7A, that arose from the whole genome duplication
S000006120	YPL199C	""	""	172033	172755	-1	Protein involved in nonstop mRNA decay	Protein involved in nonstop mRNA decay; predicted to be palmitoylated
S000006121	YPL200W	CSM4	Chromosome Segregation in Meiosis	171484	171954	1	Protein required for accurate chromosome segregation during meiosis	Protein required for accurate chromosome segregation during meiosis; involved in meiotic telomere clustering (bouquet formation) and telomere-led rapid prophase movements; functions with meiosis-specific telomere-binding protein Ndj1p; CSM4 has a paralog, MPS2, that arose from the whole genome duplication
S000006122	YPL201C	YIG1	Yeast protein Interacting with Glycerol 3-phosphatase	169770	171155	-1	Protein that interacts with glycerol 3-phosphatase	Protein that interacts with glycerol 3-phosphatase; plays a role in anaerobic glycerol production; localizes to the nucleus and cytosol
S000006123	YPL202C	AFT2	Activator of Fe (iron) Transcription	168088	169338	-1	Iron-regulated transcriptional activator	Iron-regulated transcriptional activator; activates genes involved in intracellular iron use and required for iron homeostasis and resistance to oxidative stress; AFT2 has a paralog, AFT1, that arose from the whole genome duplication
S000006124	YPL203W	TPK2	Takashi's Protein Kinase	166256	167398	1	cAMP-dependent protein kinase catalytic subunit	cAMP-dependent protein kinase catalytic subunit; promotes nutrient responsive growth via the Ras-cAMP signaling pathway; phosphorylates and inhibits histone demethylase Jhd2p in response to glucose, promoting H3K4 trimethylation and maintaining H3K14 acetylation by preventing chromatin binding of Rpd3p; required for Sfl1p-mediated pseudohyphal growth; partially redundant with Tpk1p and Tpk3p; localizes to P-bodies during stationary phase; relocalizes to the cytosol in response to hypoxia
S000006126	YPL205C	""	""	163922	164266	-1	Hypothetical protein	Hypothetical protein; deletion of locus affects telomere length
S000006127	YPL206C	PGC1	Phosphatidyl Glycerol phospholipase C	162632	163597	-1	Phosphatidylglycerol phospholipase C	Phosphatidylglycerol phospholipase C; regulates phosphatidylglycerol (PG) accumulation via a phospholipase C-type degradation mechanism; PG levels affect mitochondrial function; contains glycerophosphodiester phosphodiesterase motifs
S000006128	YPL207W	TYW1	Trna-YW synthesizing protein	159909	162341	1	Iron-sulfur protein required for synthesis of wybutosine modified tRNA	Iron-sulfur protein required for synthesis of wybutosine modified tRNA; wybutosine is a modified guanosine found at 3'-position adjacent to anticodon of phenylalanine tRNA which supports reading frame maintenance by stabilizing codon-anticodon interactions; induction by Yap5p in response to iron provides protection from high iron toxicity; overexpression results in increased cellular iron
S000006129	YPL208W	RKM1	Ribosomal lysine (K) Methyltransferase	157842	159593	1	SET-domain lysine-N-methyltransferase	SET-domain lysine-N-methyltransferase; catalyzes the formation of dimethyllysine residues on the large ribosomal subunit proteins L23 (Rpl23Ap and Rpl23Bp) and monomethyllysine residues on L18 (Rps18Ap and Rps18Bp)
S000006130	YPL209C	IPL1	Increase in PLoidy	156490	157593	-1	Aurora kinase of the chromosomal passenger complex	Aurora kinase of the chromosomal passenger complex; mediates release of mono-oriented kinetochores from microtubules in MI, and kinetochore release from SPB clusters during meiotic exit; maintains meiotic spindle checkpoint delay in a mek1 null; maintains condensed chromosomes in anaphase; required for SPB cohesion and preventing multipolar spindle formation; promotes telomerase release at G2/M; Iocalizes to nuclear foci that diffuse upon DNA replication stress; inhibits Pse1p upon SAC arrest
S000006131	YPL210C	SRP72	Signal Recognition Particle	154290	156212	-1	Core component of the signal recognition particle (SRP)	Core component of the signal recognition particle (SRP); the SRP is a ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane
S000006133	YPL212C	PUS1	PseudoUridine Synthase	151515	153149	-1	tRNA:pseudouridine synthase	tRNA:pseudouridine synthase; introduces pseudouridines at positions 26-28, 34-36, 65, and 67 of tRNA; also acts on U2 snRNA; also pseudouridylates some mRNAs, and pseudouridylation level varies with growth phase; nuclear protein that appears to be involved in tRNA export; PUS1 has a paralog, PUS2, that arose from the whole genome duplication
S000006134	YPL213W	LEA1	Looks Exceptionally like U2A	150614	151330	1	Component of U2 snRNP complex	Component of U2 snRNP complex; disruption causes reduced U2 snRNP levels; physically interacts with Msl1p; putative homolog of human U2A' snRNP protein
S000006135	YPL214C	THI6	THIamine biosynthesis	148569	150191	-1	Thiamine-phosphate diphosphorylase and hydroxyethylthiazole kinase	Thiamine-phosphate diphosphorylase and hydroxyethylthiazole kinase; required for thiamine biosynthesis; GFP-fusion protein localizes to the cytoplasm in a punctate pattern
S000006136	YPL215W	CBP3	Cytochrome B mRNA Processing	147416	148423	1	Mitochondrial protein required for assembly of cytochrome bc1 complex	Mitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex; Cbp3p-Cbp6p complex is sequestered if assembly of Complex III is blocked, downregulating COB mRNA translation
S000006137	YPL216W	""	""	143821	147129	1	Putative protein of unknown function	Putative protein of unknown function; not an essential gene; YPL216W has a paralog, ITC1, that arose from the whole genome duplication
S000006139	YPL218W	SAR1	Secretion-Associated, Ras-related	138698	139409	1	ARF family GTPase	ARF family GTPase; component of the COPII vesicle coat; required for transport vesicle formation during ER to Golgi protein transport; lowers membrane rigidity aiding vesicle formation; localizes to ER-mitochondrial contact sites where it enhances membrane curvature, thereby reducing contact size via its N-terminal amphipathic helix; regulates mitochondrial fission and fusion dynamics
S000006140	YPL219W	PCL8	Pho85 CycLin	136750	138228	1	Cyclin	Cyclin; interacts with Pho85p cyclin-dependent kinase (Cdk) to phosphorylate and regulate glycogen synthase, also activates Pho85p for Glc8p phosphorylation; PCL8 has a paralog, PCL10, that arose from the whole genome duplication
S000006141	YPL220W	RPL1A	Ribosomal Protein of the Large subunit	135790	136443	1	Ribosomal 60S subunit protein L1A	Ribosomal 60S subunit protein L1A; N-terminally acetylated; homologous to mammalian ribosomal protein L10A and bacterial L1; RPL1A has a paralog, RPL1B, that arose from the whole genome duplication; rpl1a rpl1b double null mutation is lethal; ribosomes containing Rpl1b are more efficient in translation of respiration-related proteins
S000006142	YPL221W	FLC1	FLavin Carrier	133043	135424	1	Flavin adenine dinucleotide transporter	Flavin adenine dinucleotide transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC1 has a paralog, FLC3, that arose from the whole genome duplication
S000006143	YPL222W	FMP40	Found in Mitochondrial Proteome	130162	132228	1	Mitochondrial AMPylator in the pseudokinase selenoprotein-O family	Mitochondrial AMPylator in the pseudokinase selenoprotein-O family; role in the oxidative stress response, transferring AMP from ATP to protein substrates (AMPylation) involved in redox homeostasis; regulates S-glutathionylation levels; proposed involvement in the environmental stress response
S000006144	YPL223C	GRE1	Genes de Respuesta a Estres (spanish for stress responsive genes)	128632	129138	-1	Hydrophilin essential in desiccation-rehydration process	Hydrophilin essential in desiccation-rehydration process; stress induced (osmotic, ionic, oxidative, heat shock and heavy metals); regulated by the HOG pathway; GRE1 has a paralog, SIP18, that arose from the whole genome duplication
S000006145	YPL224C	MMT2	Mitochondrial Metal Transporter	126634	128088	-1	Putative metal transporter involved in mitochondrial iron accumulation	Putative metal transporter involved in mitochondrial iron accumulation; MMT2 has a paralog, MMT1, that arose from the whole genome duplication
S000006146	YPL225W	""	""	126006	126446	1	Protein of unknown function	Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; protein abundance increases in response to DNA replication stress
S000006147	YPL226W	NEW1	Nu+	121767	125357	1	Translation termination and ribosome biogenesis factor	Translation termination and ribosome biogenesis factor; ATP binding cassette protein that cosediments with polysomes and is required for biogenesis of the small ribosomal subunit; Asn/Gln-rich rich region supports [NU+] prion formation and susceptibility to [PSI+] prion induction
S000006148	YPL227C	ALG5	Asparagine-Linked Glycosylation	120163	121167	-1	UDP-glucose:dolichyl-phosphate glucosyltransferase	UDP-glucose:dolichyl-phosphate glucosyltransferase; involved in asparagine-linked glycosylation in the endoplasmic reticulum; human ortholog ALG5 can partially complement yeast alg5 mutant
S000006149	YPL228W	CET1	Capping Enzyme Triphosphatase	118382	120031	1	RNA 5'-triphosphatase involved in mRNA 5' capping	RNA 5'-triphosphatase involved in mRNA 5' capping; subunit of mRNA capping enzyme, which is a heterotetramer composed of a Cet1p homodimer and two molecules of guanylyltransferase Ceg1p; Cet1p also has a role in regulation of RNAPII pausing at promoter-proximal sites; interaction between Cet1p and Ceg1p is required for Ceg1p nuclear import; mammalian enzyme is single bifunctional polypeptide; human homolog RNGTT can complement yeast cet1 null mutant
S000006150	YPL229W	""	""	117067	117687	1	Putative protein of unknown function	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; not an essential gene; YPL229W has a paralog, YMR181C, that arose from the whole genome duplication
S000006151	YPL230W	USV1	Up in StarVation	115312	116487	1	Putative transcription factor containing a C2H2 zinc finger	Putative transcription factor containing a C2H2 zinc finger; mutation affects transcriptional regulation of genes involved in growth on non-fermentable carbon sources, response to salt stress and cell wall biosynthesis; USV1 has a paralog, RGM1, that arose from the whole genome duplication
S000006152	YPL231W	FAS2	Fatty Acid Synthetase	108652	114315	1	Alpha subunit of fatty acid synthetase	Alpha subunit of fatty acid synthetase; complex catalyzes the synthesis of long-chain saturated fatty acids; contains the acyl-carrier protein domain and beta-ketoacyl reductase, beta-ketoacyl synthase and self-pantetheinylation activities
S000006153	YPL232W	SSO1	Supressor of Sec One	107275	108147	1	Plasma membrane t-SNARE	Plasma membrane t-SNARE; involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p; SSO1 has a paralog, SSO2, that arose from the whole genome duplication
S000006154	YPL233W	NSL1	Nnf1 Synthetic Lethal	106172	106822	1	Essential component of the MIND kinetochore complex	Essential component of the MIND kinetochore complex; joins kinetochore subunits contacting DNA to those contacting microtubules; required for accurate chromosome segregation; complex consists of Mtw1p Including Nnf1p-Nsl1p-Dsn1p (MIND)
S000006155	YPL234C	VMA11	Vacuolar Membrane Atpase	104946	105440	-1	Vacuolar ATPase V0 domain subunit c'	Vacuolar ATPase V0 domain subunit c'; involved in proton transport activity; hydrophobic integral membrane protein (proteolipid) containing four transmembrane segments; N and C termini are in the vacuolar lumen
S000006156	YPL235W	RVB2	RuVB-like	103232	104647	1	ATP-dependent DNA helicase, also known as reptin	ATP-dependent DNA helicase, also known as reptin; member of the AAA+ and RuvB protein families, similar to Rvb1p; conserved component of multiple complexes including the INO80 complex, the Swr1 complex, and the R2TP complex (Rvb1-Rvb2-Tah1-Pih1); involved in multiple processes such as chromatin remodeling, box C/D snoRNP assembly, and RNA polymerase II assembly
S000006157	YPL236C	ENV7	late ENdosome and Vacuole interface function	101608	102702	-1	Vacuolar membrane protein kinase	Vacuolar membrane protein kinase; negatively regulates membrane fusion; associates with vacuolar membrane through palmitoylation of one or more cysteines in consensus sequence; vacuolar membrane association is essential to its kinase activity; targeted to vacuole via AP-3 pathway; mutant shows defect in CPY processing; ortholog of human serine/threonine kinase 16 (STK16)
S000006159	YPL238C	""	""	100474	100863	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified essential gene SUI3/YPL237W
S000006160	YPL239W	YAR1	Yeast Ankyrin Repeat	99484	100086	1	Nucleocytoplasmic shuttling assembly chaperone	Nucleocytoplasmic shuttling assembly chaperone; co-translationally associates with nascent Rps3p in the cytoplasm and delivers it to pre-ribosomal subunits in the nucleus; required for 40S ribosomal subunit nuclear export, biogenesis and adaptation to osmotic and oxidative stress;  contains ankyrin-repeats; expression repressed by heat shock
S000006161	YPL240C	HSP82	Heat Shock Protein	96496	98625	-1	Hsp90 chaperone	Hsp90 chaperone; functionally redundant with Hsc82p; required for pheromone signaling; negative regulator of the Hsf1p-dependent heat shock response with Cpr7p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding, nucleotide addition; promotes solubility of chaperone-substrate complexes; DNA damage induced nuclear import is Aha1p dependent; protein abundance increases in response to DNA replication stress; human homolog, HSP90AB1, complements null mutant
S000006162	YPL241C	CIN2	Chromosome INstability	95372	96258	-1	GTPase-activating protein (GAP) for Cin4p	GTPase-activating protein (GAP) for Cin4p; tubulin folding factor C involved in beta-tubulin (Tub2p) folding; mutants display increased chromosome loss and benomyl sensitivity; human homolog RP2 complements yeast null mutant
S000006163	YPL242C	IQG1	IQGAP-related protein	90622	95109	-1	Actin filament binding protein that enhances actin ring formation	Actin filament binding protein that enhances actin ring formation; IQGAP family member required for assembly and contraction of the AMR, prior to cytokinesis; localizes to the contractile ring during anaphase in an Mlc1p-dependent manner, recruiting Myo1p and Hof1p to the site, and promoting cytokinetic core complex assembly; specifies bud-site selection, localizing axial markers Bud4p and Cdc12p; regulated by Cdc28p; relocalizes from the bud neck to the cytoplasm upon replication stress
S000006164	YPL243W	SRP68	Signal Recognition Particle	88517	90316	1	Core component of the signal recognition particle (SRP) complex	Core component of the signal recognition particle (SRP) complex; SRP complex functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane; relocalizes from cytoplasm to the nuclear periphery upon DNA replication stress
S000006165	YPL244C	HUT1	Homolog of UDP-galactose Transporter	87014	88033	-1	Protein with a role in UDP-galactose transport to the Golgi lumen	Protein with a role in UDP-galactose transport to the Golgi lumen; has similarity to human UDP-galactose transporter UGTrel1, exhibits a genetic interaction with S. cerevisiae ERO1
S000006166	YPL245W	""	""	85586	86950	1	Putative protein of unknown function	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to both the nucleus and the cytoplasm
S000006167	YPL246C	RBD2	""	84509	85297	-1	Possible rhomboid protease	Possible rhomboid protease; has similarity to eukaryotic rhomboid proteases including Pcp1p
S000006168	YPL247C	""	""	82625	84196	-1	Putative protein of unknown function	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; similar to the petunia WD repeat protein an11; overexpression causes a cell cycle delay or arrest
S000006169	YPL248C	GAL4	GALactose metabolism	79711	82356	-1	DNA-binding transcription factor required for activating GAL genes	DNA-binding transcription factor required for activating GAL genes; responds to galactose; repressed by Gal80p and activated by Gal3p
S000006170	YPL249C	GYP5	Gtpase-activating protein for Ypt Proteins	76669	79353	-1	GTPase-activating protein (GAP) for yeast Rab family members	GTPase-activating protein (GAP) for yeast Rab family members; involved in ER to Golgi trafficking; exhibits GAP activity toward Ypt1p that is stimulated by Gyl1p, also acts on Sec4p; interacts with Gyl1p, Rvs161p and Rvs167p; involved in recruiting Rvs167p to the bud tip during polarized growth; relocalizes from bud neck to cytoplasm upon DNA replication stress; GYP5 has a paralog, GYL1, that arose from the whole genome duplication
S000006171	YPL250C	ATG41	AuTophaGy related	74309	74719	-1	Protein of unknown function	Protein of unknown function; required for selective and nonselective autophagy, and mitophagy; regulates the rate of autophagosome formation; interacts with Atg9p, and has a similar peri-mitochondrial localization; elevated Gcn4p-dependent expression under autophagy-inducing conditions; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate; ATG41 has a paralog, ICY1, that arose from the whole genome duplication
S000006173	YPL252C	YAH1	Yeast Adrenodoxin Homolog	73363	73881	-1	Ferredoxin of the mitochondrial matrix	Ferredoxin of the mitochondrial matrix; required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; human homolog FDX1L can complement yeast by allowing growth during down-regulation of yeast YAH1
S000006174	YPL253C	VIK1	Vegetative Interaction with Kar3p	71063	73006	-1	Protein that forms a kinesin-14 heterodimeric motor with Kar3p	Protein that forms a kinesin-14 heterodimeric motor with Kar3p; localizes Kar3p at mitotic spindle poles; has a structure similar to a kinesin motor domain but lacks an ATP-binding site and is catalytically inactive; binds microtubules; required for sister chromatid cohesion; VIK1 has a paralog, CIK1, that arose from the whole genome duplication
S000006175	YPL254W	HFI1	Histone H2A Functional Interactor	69485	70951	1	Adaptor protein required for structural integrity of the SAGA complex	Adaptor protein required for structural integrity of the SAGA complex; a histone acetyltransferase-coactivator complex that is involved in global regulation of gene expression through acetylation and transcription functions
S000006176	YPL255W	BBP1	Bfr1 Binding Protein	67725	68882	1	Protein required for the spindle pole body (SPB) duplication	Protein required for the spindle pole body (SPB) duplication; localizes at the cytoplasmic side of the central plaque periphery of the SPB; forms a complex with a nuclear envelope protein Mps2p and SPB components Spc29p and Kar1p; required for mitotic functions of Cdc5p
S000006177	YPL256C	CLN2	CycLiN	64977	66614	-1	G1 cyclin involved in regulation of the cell cycle	G1 cyclin involved in regulation of the cell cycle; activates Cdc28p kinase to promote the G1 to S phase transition; late G1 specific expression depends on transcription factor complexes, MBF (Swi6p-Mbp1p) and SBF (Swi6p-Swi4p); CLN2 has a paralog, CLN1, that arose from the whole genome duplication; cell cycle arrest phenotype of the cln1 cln2 cln3 triple null mutant is complemented by any of human cyclins CCNA2, CCNB1, CCNC, CCND1, or CCNE1
S000006178	YPL257W	""	""	63279	63860	1	Putative protein of unknown function	Putative protein of unknown function; homozygous diploid deletion strain exhibits low budding index; physically interacts with Hsp82p; YPL257W is not an essential gene
S000006179	YPL258C	THI21	THIamine metabolism	53498	55153	-1	Hydroxymethylpyrimidine (HMP) and HMP-phosphate kinase	Hydroxymethylpyrimidine (HMP) and HMP-phosphate kinase; involved in thiamine biosynthesis; member of a gene family with THI20 and THI22; functionally redundant with Thi20p
S000006180	YPL259C	APM1	clathrin Adaptor Protein complex Medium chain	51244	52671	-1	Mu1-like medium subunit of the AP-1 complex	Mu1-like medium subunit of the AP-1 complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; the AP-1 complex is the clathrin-associated protein complex
S000006181	YPL260W	CUB1	Cu2+ suppressing and Bleomycin sensitive	49303	50958	1	Conserved fungal gene linked to DNA repair and proteasome function	Conserved fungal gene linked to DNA repair and proteasome function; putative substrate of cAMP-dependent protein kinase (PKA); green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YPL260W is not an essential gene; protein abundance increases in response to DNA replication stress
S000006182	YPL261C	""	""	48996	49304	-1	Putative protein of unknown function	Putative protein of unknown function; conserved among S. cerevisiae strains; YPL261C is not an essential gene; partially overlaps verified ORF YPL260W
S000006183	YPL262W	FUM1	FUMarase	47336	48802	1	Fumarase	Fumarase; converts fumaric acid to L-malic acid in the TCA cycle; cytosolic and mitochondrial distribution determined by the N-terminal targeting sequence, protein conformation, and status of glyoxylate shunt; phosphorylated in mitochondria
S000006184	YPL263C	KEL3	KELch	44551	46506	-1	Cytoplasmic protein of unknown function	Cytoplasmic protein of unknown function
S000006185	YPL264C	""	""	43283	44344	-1	Endoplasmic reticulum protein of unknown function	Endoplasmic reticulum protein of unknown function; physically interacts with Hsp82p; YPL264C is not an essential gene
S000006186	YPL265W	DIP5	DIcarboxylic amino acid Permease	41043	42869	1	Dicarboxylic amino acid permease	Dicarboxylic amino acid permease; mediates high-affinity and high-capacity transport of L-glutamate and L-aspartate; also a transporter for Gln, Asn, Ser, Ala, and Gly; relocalizes from plasma membrane to vacuole upon DNA replication stress
S000006187	YPL266W	DIM1	DIMethylase	39121	40077	1	Essential 18S rRNA dimethylase (dimethyladenosine transferase)	Essential 18S rRNA dimethylase (dimethyladenosine transferase); responsible for conserved m6(2)Am6(2)A dimethylation in 3'-terminal loop of 18S rRNA, part of 90S and 40S pre-particles in nucleolus, involved in pre-ribosomal RNA processing; human homolog DIMT1 complements yeast dim1 mutant
S000006188	YPL267W	ACM1	APC/C[Cdh1] Modulator	38169	38798	1	Pseudosubstrate inhibitor of the APC/C	Pseudosubstrate inhibitor of the APC/C; suppresses APC/C [Cdh1]-mediated proteolysis of mitotic cyclins; associates with Cdh1p, Bmh1p and Bmh2p; cell cycle regulated protein; the anaphase-promoting complex/cyclosome is also known as APC/C
S000006189	YPL268W	PLC1	PhosphoLipase C	35236	37845	1	Phospholipase C	Phospholipase C; hydrolyzes phosphatidylinositol 4,5-biphosphate (PIP2) to generate the signaling molecules inositol 1,4,5-triphosphate (IP3) and 1,2-diacylglycerol (DAG); involved in regulating many cellular processes; Plc1p and inositol polyphosphates are required for acetyl-CoA homeostasis which regulates global histone acetylation
S000006190	YPL269W	KAR9	KARyogamy	33013	34947	1	Spindle positioning factor	Spindle positioning factor; orients astral microtubules, connecting them to actin cables at the cortex with Bim1p and Myo2, resulting in proper spindle positioning; targeted for StuBL-dependent degradation at kinetochores by Slx5p-Slx8p, ensuring chromosome transmission fidelity and correct spindle positioning; role in karyogamy; localizes to the shmoo tip, the growing bud-tip, the nucleus, the kinetochore, the spindle and microtubules; homolog of adenomatous polyposis coli
S000006191	YPL270W	MDL2	MultiDrug resistance-Like	30482	32803	1	Mitochondrial inner membrane half-type ABC transporter	Mitochondrial inner membrane half-type ABC transporter; required for respiratory growth at high temperature; localizes to vacuole membrane in response to H2O2; similar to human TAP1 and TAP2 implicated in bare lymphocyte syndrome and Wegener-like granulomatosis
S000006192	YPL271W	ATP15	ATP synthase	30079	30267	1	Epsilon subunit of the F1 sector of mitochondrial F1F0 ATP synthase	Epsilon subunit of the F1 sector of mitochondrial F1F0 ATP synthase; which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; F1 translationally regulates ATP6 and ATP8 expression to achieve a balanced output of ATP synthase genes encoded in nucleus and mitochondria; phosphorylated
S000006193	YPL272C	PBI1	PSTB2 Interacting protein	26611	28164	-1	Putative protein of unknown function	Putative protein of unknown function; gene expression induced in response to ketoconazole; YPL272C is not an essential gene
S000006194	YPL273W	SAM4	S-AdenosylMethionine metabolism	25087	26064	1	S-adenosylmethionine-homocysteine methyltransferase	S-adenosylmethionine-homocysteine methyltransferase; functions along with Mht1p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio; SAM4 has a paralog, YMR321C, that arose from a single-locus duplication
S000006195	YPL274W	SAM3	S-AdenosylMethionine metabolism	22938	24701	1	High-affinity S-adenosylmethionine permease	High-affinity S-adenosylmethionine permease; required for utilization of S-adenosylmethionine as a sulfur source; has similarity to S-methylmethionine permease Mmp1p
S000006198	YPL277C	""	""	15405	16868	-1	Putative protein of unknown function	Putative protein of unknown function; localized to the membranes; gene expression regulated by copper levels
S000006205	YPR001W	CIT3	CITrate synthase	556377	557837	1	Dual specificity mitochondrial citrate and methylcitrate synthase	Dual specificity mitochondrial citrate and methylcitrate synthase; catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate and that of propionyl-CoA and oxaloacetate to form 2-methylcitrate
S000006206	YPR002W	PDH1	prpD Homolog	558385	559935	1	Putative 2-methylcitrate dehydratase	Putative 2-methylcitrate dehydratase; mitochondrial protein that participates in respiration; induced by diauxic shift; homologous to E. coli PrpD, may take part in the conversion of 2-methylcitrate to 2-methylisocitrate
S000006207	YPR003C	""	""	561504	563768	-1	Putative sulfate permease	Putative sulfate permease; physically interacts with Hsp82p; green fluorescent protein (GFP)-fusion protein localizes to the ER; YPR003C is not an essential gene
S000006208	YPR004C	AIM45	Altered Inheritance rate of Mitochondria	564007	565041	-1	Putative ortholog of mammalian ETF-alpha	Putative ortholog of mammalian ETF-alpha; interacts with frataxin, Yfh1p; null mutant displays elevated frequency of mitochondrial genome loss; may have a role in oxidative stress response; ETF-alpha is an electron transfer flavoprotein complex subunit
S000006209	YPR005C	HAL1	HALotolerance	565787	566671	-1	Cytoplasmic protein involved in halotolerance	Cytoplasmic protein involved in halotolerance; decreases intracellular Na+ (via Ena1p) and increases intracellular K+ by decreasing efflux; expression repressed by Ssn6p-Tup1p and Sko1p and induced by NaCl, KCl, and sorbitol through Gcn4p
S000006210	YPR006C	ICL2	IsoCitrate Lyase	567269	568996	-1	2-methylisocitrate lyase of the mitochondrial matrix	2-methylisocitrate lyase of the mitochondrial matrix; functions in the methylcitrate cycle to catalyze the conversion of 2-methylisocitrate to succinate and pyruvate; ICL2 transcription is repressed by glucose and induced by ethanol
S000006211	YPR007C	REC8	RECombination	569336	571378	-1	Meiosis-specific component of the sister chromatid cohesion complex	Meiosis-specific component of the sister chromatid cohesion complex; alpha-kleisin family member that maintains cohesion between homologous chromosomes during meiosis I until chromosome arm-associated Rec8p is cleaved by Esp1p; cohesion between sister chromatid centromeres is maintained until metaphase of meiosis II when it is cleaved by Esp1; independent of the role in cohesion, Rec8p promotes allelic collisions and prevents nonspecific chromosome interactions; homolog of S. pombe Rec8p
S000006212	YPR008W	HAA1	Homolog of Ace1 Activator	573018	575102	1	Transcriptional activator involved in adaptation to weak acid stress	Transcriptional activator involved in adaptation to weak acid stress; activates transcription of TPO2, YRO2, and other genes encoding membrane stress proteins; HAA1 has a paralog, CUP2, that arose from the whole genome duplication; relocalizes from cytoplasm to nucleus upon DNA replication stress
S000006213	YPR009W	SUT2	Sterol UpTake	576552	577358	1	Zn2Cys6 family transcription factor	Zn2Cys6 family transcription factor; positively regulates sterol uptake under anaerobic conditions with SUT1; represses filamentation-inducing genes during non-starvation conditions; positively regulates mating along with SUT1 by repressing the expression of genes (PRR2, NCE102 and RHO5) which function as mating inhibitors; multicopy suppressor of mutations that cause low activity of the cAMP/PKA pathway; SUT2 has a paralog, SUT1, that arose from the whole genome duplication
S000006215	YPR011C	MRX21	Mitochondrial oRganization of gene eXpression (MIOREX)	583062	584042	-1	Mitochondrial transporter	Mitochondrial transporter; major substrates are adenosine 5'-phosphosulfate (APS) and 3'-phospho-adenosine 5'-phosphosulfate (PAPS); member of the mitochondrial carrier family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
S000006216	YPR012W	""	""	584309	584563	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; YPR012W is not an essential gene
S000006217	YPR013C	CMR3	Changed Mutation Rate	584632	585585	-1	Putative zinc finger protein	Putative zinc finger protein; YPR013C is not an essential gene
S000006218	YPR014C	""	""	587189	587518	-1	Putative protein of unknown function	Putative protein of unknown function; conserved across S. cerevisiae strains; YPR014C is not an essential gene
S000006219	YPR015C	""	""	590283	591026	-1	Putative zinc finger transcription factor	Putative zinc finger transcription factor; binds DNA in sequence-specific manner; overexpression causes a cell cycle delay or arrest
S000006220	YPR016C	TIF6	Translation Initiation Factor	592332	593069	-1	Constituent of 66S pre-ribosomal particles	Constituent of 66S pre-ribosomal particles; has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits
S000006221	YPR017C	DSS4	Dominant Suppressor of Sec4	593486	593917	-1	Guanine nucleotide dissociation stimulator for Sec4p	Guanine nucleotide dissociation stimulator for Sec4p; functions in the post-Golgi secretory pathway; binds zinc, found both on membranes and in the cytosol
S000006222	YPR018W	RLF2	Rap1 protein Localization Factor	594476	596296	1	Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1)	Largest subunit (p90) of the Chromatin Assembly Complex (CAF-1); chromatin assembly by CAF-1 is important for multiple processes including histone H3/H4 tetramerization, silencing at telomeres, mating type loci and rDNA, maintenance of kinetochore structure, deactivation of  DNA damage checkpoint after DNA repair, chromatin dynamics during transcription, and repression of divergent noncoding transcription
S000006224	YPR020W	ATP20	ATP synthase	599870	600217	1	Subunit g of the mitochondrial F1F0 ATP synthase	Subunit g of the mitochondrial F1F0 ATP synthase; reversibly phosphorylated on two residues; unphosphorylated form is required for dimerization of the ATP synthase complex, which in turn determines oligomerization of the complex and the shape of inner membrane cristae
S000006225	YPR021C	AGC1	Aspartate-Glutamate Carrier	600649	603357	-1	Mitochondrial amino acid transporter	Mitochondrial amino acid transporter; acts both as a glutamate uniporter and as an aspartate-glutamate exchanger; involved in nitrogen metabolism and nitrogen compound biosynthesis; human homolog SLC25A13 complements yeast null mutant
S000006226	YPR022C	SDD4	Suppressor of Degenerative Death	603911	607312	-1	Putative transcription factor, as suggested by computational analysis	Putative transcription factor, as suggested by computational analysis; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus and is induced in response to the DNA-damaging agent MMS; overproduction of a truncation allele suppresses lethality due to expression of the dominant PET9 allele AAC2-A128P
S000006227	YPR023C	EAF3	Esa1p-Associated Factor	608826	610031	-1	Component of the Rpd3S histone deacetylase complex	Component of the Rpd3S histone deacetylase complex; Esa1p-associated factor, nonessential component of the NuA4 acetyltransferase complex, homologous to Drosophila dosage compensation protein MSL3; plays a role in regulating Ty1 transposition
S000006228	YPR024W	YME1	Yeast Mitochondrial Escape	610481	612724	1	Catalytic subunit of i-AAA protease complex	Catalytic subunit of i-AAA protease complex; complex is located in mitochondrial inner membrane; responsible for degradation of unfolded or misfolded mitochondrial gene products; serves as nonconventional translocation motor to pull PNPase into intermembrane space; also has role in intermembrane space protein folding; mutation causes elevated rate of mitochondrial turnover; human homolog YME1L1 can complement yeast null mutant
S000006229	YPR025C	CCL1	""	613377	614558	-1	Cyclin partner of the cyclin-dependent kinase (CDK) Kin28p	Cyclin partner of the cyclin-dependent kinase (CDK) Kin28p; regulates the activity of Kin28p, a TFIIH-associated carboxy-terminal domain (CTD) kinase that facilitates recruitment of mRNA 5'-capping and polyadenylation factors to the RNAPII holoenzyme complex, and has a minor role in RNAPII transcription; subunit of TFIIK, a TFIIH subassembly;  human homolog CCNH allows growth of a yeast ccl1 temperature-sensitive mutant at restrictive temperature
S000006230	YPR026W	ATH1	Acid TreHalase	615379	619014	1	Acid trehalase required for utilization of extracellular trehalose	Acid trehalase required for utilization of extracellular trehalose; involved in intracellular trehalose degradation during growth recovery after saline stress
S000006231	YPR027C	""	""	620425	621258	-1	Putative protein of unknown function	Putative protein of unknown function; SWAT-GFP and seamless-GFP fusion proteins localize to the endoplasmic reticulum, while the mCherry fusion protein localizes to the vacuole
S000006232	YPR028W	YOP1	YIP One Partner	623527	624202	1	Reticulon-interacting protein	Reticulon-interacting protein; ER integral membrane protein involved in the generation of tubular ER morphology; promotes membrane curvature; forms tubules in vitro; regulates the ER asymmetry-induced inheritance block during ER stress; role in ER-derived peroxisomal biogenesis; interacts with Yip1p to mediate membrane traffic and with Sey1p to maintain ER morphology; facilitates lipid exchange between the ER and mitochondria; forms ER foci upon DNA replication stress
S000006233	YPR029C	APL4	clathrin Adaptor Protein complex Large chain	624469	626967	-1	Gamma-adaptin	Gamma-adaptin; large subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in vesicle mediated transport
S000006234	YPR030W	CSR2	Chs5 Spa2 Rescue	627880	631245	1	Nuclear ubiquitin protein ligase binding protein	Nuclear ubiquitin protein ligase binding protein; may regulate utilization of nonfermentable carbon sources and endocytosis of plasma membrane proteins; overproduction suppresses chs5 spa2 lethality at high temp; ubiquitinated by Rsp5p, deubiquitinated by Ubp2p; CSR2 has a paralog, ECM21, that arose from the whole genome duplication
S000006235	YPR031W	NTO1	NuA Three Orf	631515	633761	1	Subunit of the NuA3 histone acetyltransferase complex	Subunit of the NuA3 histone acetyltransferase complex; this complex acetylates histone H3; contains PHD finger domain that interacts with methylated histone H3
S000006236	YPR032W	SRO7	Suppressor of rho3	634123	637224	1	Effector of Rab GTPase Sec4p	Effector of Rab GTPase Sec4p; forms a complex with Sec4p and t-SNARE Sec9p; involved in exocytosis and docking and fusion of post-Golgi vesicles with plasma membrane; regulates cell proliferation and colony development via the Rho1-Tor1 pathway; homolog of Drosophila lgl tumor suppressor; SRO7 has a paralog, SRO77, that arose from the whole genome duplication
S000006237	YPR033C	HTS1	Histidine-Trna Synthetase	637379	639019	-1	Cytoplasmic and mitochondrial histidine tRNA synthetase	Cytoplasmic and mitochondrial histidine tRNA synthetase; efficient mitochondrial localization requires both a presequence and an amino-terminal sequence; mutations in human ortholog HARS2 are associated with Perrault syndrome
S000006238	YPR034W	ARP7	Actin-Related Protein	639525	640958	1	Component of both the SWI/SNF and RSC chromatin remodeling complexes	Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation
S000006239	YPR035W	GLN1	GLutamiNe metabolism	642208	643320	1	Glutamine synthetase (GS)	Glutamine synthetase (GS); synthesizes glutamine from glutamate and ammonia; with Glt1p, forms the secondary pathway for glutamate biosynthesis from ammonia; expression regulated by nitrogen source and by amino acid limitation; forms filaments of back-to-back stacks of cylindrical homo-decamers at low pH, leading to enzymatic inactivation and storage during states of advanced cellular starvation; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
S000006240	YPR036W	VMA13	Vacuolar Membrane Atpase	643836	645272	1	Subunit H of the V1 peripheral membrane domain of V-ATPase	Subunit H of the V1 peripheral membrane domain of V-ATPase; part of the electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase; the V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase) has eight subunits
S000006241	YPR037C	ERV2	Essential for Respiration and Viability	646448	647038	-1	Flavin-linked sulfhydryl oxidase localized to the ER lumen	Flavin-linked sulfhydryl oxidase localized to the ER lumen; involved in disulfide bond formation within the endoplasmic reticulum (ER)
S000006242	YPR038W	IRC16	Increased Recombination Centers	646836	647195	1	Putative protein of unknown function	Putative protein of unknown function; partially overlaps verified gene ERV2/YPR037C but doesn't share phenotypes; also overlaps dubious ORF YPR039W; null mutant displays increased levels of spontaneous Rad52p foci, increased biofilm information, different altered sensitivities to various chemcicals
S000006243	YPR039W	""	""	647012	647347	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified non essential genes ERV2/YPR037C and TIP41/YPR040W
S000006244	YPR040W	TIP41	Tap42 Interacting Protein	647305	648375	1	Tap42p-interacting protein	Tap42p-interacting protein; negative regulator of the TORC1 signaling pathway that activates the type 2A-related phosphatase Sit4p by binding and antagonizing Tap42p, a SIT4 inhibitor; proposed to be part of a feedback loop to amplify Sit4p activity when TORC1 is inactivated; protein abundance increases in response to DNA replication stress
S000006245	YPR041W	TIF5	Translation Initiation Factor	648704	649921	1	Translation initiation factor eIF5	Translation initiation factor eIF5; functions as both a GTPase-activating protein (GAP) that stimulates the hydrolysis of GTP bound to eIF-2, as part of the 43S preinitiation complex, and as a GDP dissociation inhibitor (GDI) to prevent recycling of eIF2; human ortholog eIF5 complements the yeast null mutant
S000006246	YPR042C	PUF2	PUmilio-homology domain Family	650435	653662	-1	PUF family mRNA-binding protein	PUF family mRNA-binding protein; Pumilio homology domain confers RNA binding activity; preferentially binds mRNAs encoding membrane-associated proteins; binding site composed of two UAAU tetranucleotides, separated by a 3-nt linker; PUF2 has a paralog, JSN1, that arose from the whole genome duplication
S000006247	YPR043W	RPL43A	Ribosomal Protein of the Large subunit	654166	654847	1	Ribosomal 60S subunit protein L43A	Ribosomal 60S subunit protein L43A; null mutation confers a dominant lethal phenotype; homologous to mammalian ribosomal protein L37A, no bacterial homolog; RPL43A has a paralog, RPL43B, that arose from the whole genome duplication
S000006248	YPR044C	OPI11	OverProducer of Inositol	654524	654877	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; largely overlaps verified gene RPL43A/YPR043W; deletion confers sensitivity to GSAO
S000006249	YPR045C	THP3	THO-related Protein	655140	656552	-1	Protein that may have a role in transcription elongation	Protein that may have a role in transcription elongation; forms a complex with Csn12p that is recruited to transcribed genes; possibly involved in splicing based on pre-mRNA accumulation defect for many intron-containing genes
S000006250	YPR046W	MCM16	MiniChromosome Maintenance	656799	657344	1	Component of the Ctf19 complex and the COMA subcomplex	Component of the Ctf19 complex and the COMA subcomplex; involved in kinetochore-microtubule mediated chromosome segregation; binds to centromere DNA; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-H and fission yeast fta3
S000006251	YPR047W	MSF1	Mitochondrial aminoacyl-tRNA Synthetase, Phenylalanine (F)	657529	658938	1	Mitochondrial phenylalanyl-tRNA synthetase	Mitochondrial phenylalanyl-tRNA synthetase; active as a monomer, unlike the cytoplasmic subunit which is active as a dimer complexed to a beta subunit dimer; similar to the alpha subunit of E. coli phenylalanyl-tRNA synthetase
S000006252	YPR048W	TAH18	Top1T722A mutant Hypersensitive	659182	661053	1	Conserved NAPDH-dependent diflavin reductase	Conserved NAPDH-dependent diflavin reductase; component of an early step in the cytosolic Fe-S protein assembly (CIA) machinery; transfers electrons from NADPH to the Fe-S cluster of Dre2p; plays a pro-death role under oxidative stress; Tah18p-dependent nitric oxide synthesis confers high-temperature stress tolerance; possible target for development of antifungal drugs
S000006253	YPR049C	ATG11	AuTophaGy related	661137	664673	-1	Adapter protein for pexophagy and the Cvt targeting pathway	Adapter protein for pexophagy and the Cvt targeting pathway; directs receptor-bound cargo to the phagophore assembly site (PAS) for packaging into vesicles; required for recruiting other proteins to the PAS; recruits Dnm1p to facilitate fission of mitochondria that are destined for removal by mitophagy
S000006254	YPR050C	""	""	664953	665366	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps verified ORF MAK3/YPR051W
S000006255	YPR051W	MAK3	MAintenance of Killer	664960	665490	1	Catalytic subunit of the NatC type N-terminal acetyltransferase (NAT)	Catalytic subunit of the NatC type N-terminal acetyltransferase (NAT); involved in subcellular targeting of select N-terminally acetylated substrates to the Golgi apparatus (Arl3p and Grh1p) and the inner nuclear membrane (Trm1p); required for replication of dsRNA virus; human NatC ortholog, NAA30, functionally complements the null, requiring either auxiliary subunit Mak10p or co-expression of human ortholog, NAA35; NAA60, the human NatF gene, also complements the null allele
S000006256	YPR052C	NHP6A	Non-Histone Protein	665693	665974	-1	High-mobility group (HMG) protein	High-mobility group (HMG) protein; binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to chromosomes; functionally redundant with Nhp6Bp; required for transcriptional initiation fidelity of some tRNA genes; homologous to mammalian HMGB1 and HMGB2; NHP6A has a paralog, NHP6B, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
S000006257	YPR053C	""	""	665788	666243	-1	Putative protein of unknown function	Putative protein of unknown function; conserved among S. cerevisiae strains; YPR053C is not an essential gene; partially overlaps verified ORF NHP6A/YPR052C
S000006258	YPR054W	SMK1	""	666280	667446	1	Middle sporulation-specific mitogen-activated protein kinase (MAPK)	Middle sporulation-specific mitogen-activated protein kinase (MAPK); required for prospore membrane development and the production of outer spore wall layers; negatively regulates activity of the glucan synthase subunit Gsc2p; activated by Cak1p-mediated phosphorylation of threonine (T207) in the activation loop during meiosis I followed by Ssp2p-promoted autophosphorylation of tyrosine (T209) during meiosis II
S000006260	YPR056W	TFB4	Transcription Factor B subunit 4	671126	672142	1	Subunit of TFIIH complex	Subunit of TFIIH complex; involved in transcription initiation, similar to 34 kDa subunit of human TFIIH; interacts with Ssl1p
S000006261	YPR057W	BRR1	Bad Response to Refrigeration	672471	673496	1	snRNP protein component of spliceosomal snRNPs	snRNP protein component of spliceosomal snRNPs; required for pre-mRNA splicing and snRNP biogenesis; in null mutant newly-synthesized snRNAs are destabilized and 3'-end processing is slowed
S000006262	YPR058W	YMC1	Yeast Mitochondrial Carrier	673751	674674	1	Secondary mitochondrial inner membrane glycine transporter	Secondary mitochondrial inner membrane glycine transporter; required with HEM25 for the transport of glycine into mitochondria for the initiation of heme biosynthesis; proposed role in oleate metabolism and glutamate biosynthesis; member of the mitochondrial carrier (MCF) family; localizes to the vacuole in response to H2O2; YMC1 has a paralog, YMC2, that arose from the whole genome duplication
S000006263	YPR059C	""	""	674314	674700	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YMC1/YPR058W
S000006264	YPR060C	ARO7	AROmatic amino acid requiring	674861	675631	-1	Chorismate mutase	Chorismate mutase; catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis
S000006265	YPR061C	JID1	DnaJ protein Involved in ER-associated Degradation	675977	676882	-1	Probable Hsp40p co-chaperone	Probable Hsp40p co-chaperone; has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae
S000006266	YPR062W	FCY1	FluoroCYtosine resistance	677165	677641	1	Cytosine deaminase	Cytosine deaminase; zinc metalloenzyme that catalyzes the hydrolytic deamination of cytosine to uracil; of biomedical interest because it also catalyzes the deamination of 5-fluorocytosine (5FC) to form anticancer drug 5-fluorouracil (5FU)
S000006267	YPR063C	""	""	677812	678320	-1	ER-localized protein of unknown function	ER-localized protein of unknown function
S000006268	YPR064W	""	""	678951	679370	1	Putative protein of unknown function	Putative protein of unknown function; conserved among S. cerevisiae strains; YPR064W is not an essential gene
S000006269	YPR065W	ROX1	Regulation by OXygen	679693	680799	1	Heme-dependent repressor of hypoxic genes	Heme-dependent repressor of hypoxic genes; mediates aerobic transcriptional repression of hypoxia induced genes such as COX5b and CYC7; repressor function regulated through decreased promoter occupancy in response to oxidative stress; contains an HMG domain that is responsible for DNA bending activity; involved in the hyperosmotic stress resistance
S000006270	YPR066W	UBA3	UBiquitin-like protein-Activating enzyme	681213	682112	1	Protein that activates Rub1p (NEDD8) before neddylation	Protein that activates Rub1p (NEDD8) before neddylation; acts together with Ula1p; may play a role in protein degradation; GFP-fusion protein localizes to the cytoplasm in a punctate pattern 
S000006271	YPR067W	ISA2	Iron Sulfur Assembly	682220	682777	1	Protein required for maturation of mitochondrial [4Fe-4S] proteins	Protein required for maturation of mitochondrial [4Fe-4S] proteins; functions in a complex with Isa1p and possibly Iba57p; localizes to the mitochondrial intermembrane space, overexpression of ISA2 suppresses grx5 mutations
S000006272	YPR068C	HOS1	Hda One Similar	682941	684353	-1	Class I histone deacetylase (HDAC) family member	Class I histone deacetylase (HDAC) family member; deacetylates Smc3p on lysine residues at anaphase onset; has sequence similarity to Hda1p, Rpd3p, Hos2p, and Hos3p; interacts with the Tup1p-Ssn6p corepressor complex
S000006273	YPR069C	SPE3	SPErmidine auxotroph	684556	685437	-1	Spermidine synthase	Spermidine synthase; involved in biosynthesis of spermidine and also in biosynthesis of pantothenic acid; spermidine is required for growth of wild-type cells
S000006274	YPR070W	MED1	MEDiator complex	685898	687598	1	Subunit of the RNA polymerase II mediator complex	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
S000006275	YPR071W	""	""	688172	688807	1	Putative membrane protein	Putative membrane protein; YPR071W is not an essential gene; YPR071W has a paralog, YIL029C, that arose from a single-locus duplication
S000006276	YPR072W	NOT5	Negative On TATA	690107	691789	1	Component of the CCR4-NOT core complex, involved in mRNA decapping	Component of the CCR4-NOT core complex, involved in mRNA decapping; involved intranscription initiation and elongation and in mRNA degradation; conserved lysine in human homolog of Not5p and Not3p is mutated in cancers
S000006277	YPR073C	LTP1	Low molecular weight protein Tyrosine Phosphatase	691933	692418	-1	Protein phosphotyrosine phosphatase of unknown cellular role	Protein phosphotyrosine phosphatase of unknown cellular role; activated by adenine
S000006278	YPR074C	TKL1	TransKetoLase	692796	694838	-1	Transketolase	Transketolase; catalyzes conversion of xylulose-5-phosphate and ribose-5-phosphate to sedoheptulose-7-phosphate and glyceraldehyde-3-phosphate in the pentose phosphate pathway; needed for synthesis of aromatic amino acids; TKL1 has a paralog, TKL2, that arose from the whole genome duplication
S000006279	YPR075C	OPY2	Overproduction-induced Pheromone-resistant Yeast	695737	696819	-1	Integral membrane protein that acts as a membrane anchor for Ste50p	Integral membrane protein that acts as a membrane anchor for Ste50p; involved in the signaling branch of the high-osmolarity glycerol (HOG) pathway and as a regulator of the filamentous growth pathway; overproduction blocks cell cycle arrest in the presence of mating pheromone; relocalizes from vacuole to plasma membrane upon DNA replication stress
S000006280	YPR076W	""	""	696476	696850	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000006281	YPR077C	""	""	697131	697502	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; expression increased by deletion of NAP1
S000006282	YPR078C	""	""	697147	698265	-1	Putative protein of unknown function	Putative protein of unknown function; possible role in DNA metabolism and/or in genome stability; expression is heat-inducible
S000006283	YPR079W	MRL1	Mannose 6-phosphate Receptor Like	698869	700014	1	Membrane protein	Membrane protein; has similarity to mammalian mannose-6-phosphate receptors; possibly functions as a sorting receptor in the delivery of vacuolar hydrolases; protein abundance increases in response to DNA replication stress
S000006286	YPR082C	DIB1	S. pombe DIm1+ in Budding yeast	704225	704656	-1	17-kDa component of the U4/U6aU5 tri-snRNP	17-kDa component of the U4/U6aU5 tri-snRNP; plays an essential role in pre-mRNA splicing; human ortholog TXNL4A (the human U5-specific 15-kDa protein) complements yeast dib1 null mutant
S000006287	YPR083W	MDM36	Mitochondrial Distribution and Morphology	704854	706593	1	Mitochondrial protein	Mitochondrial protein; required for normal mitochondrial morphology and inheritance; component of the mitochondria-ER-cortex-anchor (MECA); interacts with Num1p to link the ER and mitochondria at the cell cortex; proposed involvement in the formation of Dnm1p and Num1p-containing cortical anchor complexes that promote mitochondrial fission
S000006288	YPR084W	""	""	706972	708342	1	Putative protein of unknown function	Putative protein of unknown function
S000006289	YPR085C	ASA1	AStra Associated protein	708497	709828	-1	Subunit of the ASTRA complex, involved in chromatin remodeling	Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1
S000006290	YPR086W	SUA7	Suppressor of Upstream AUG	710101	711138	1	Transcription factor TFIIB	Transcription factor TFIIB; a general transcription factor required for transcription initiation and start site selection by RNA polymerase II
S000006291	YPR087W	VPS69	Vacuolar Protein Sorting	711354	711674	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; not conserved in closely related Saccharomyces species; 85% of ORF overlaps the verified gene SRP54; deletion causes a vacuolar protein sorting defect
S000006292	YPR088C	SRP54	Signal Recognition Particle 54-kD subunit	711403	713028	-1	Signal recognition particle (SRP) subunit (homolog of mammalian SRP54)	Signal recognition particle (SRP) subunit (homolog of mammalian SRP54); contains the signal sequence-binding activity of SRP, interacts with the SRP RNA, and mediates binding of SRP to signal receptor; contains GTPase domain
S000006293	YPR089W	""	""	713275	715941	1	Protein of unknown function	Protein of unknown function; exhibits genetic interaction with ERG11 and protein-protein interaction with Hsp82p
S000006294	YPR090W	""	""	713728	715938	1	Merged open reading frame	Merged open reading frame; does not encode a discrete protein; YPR090W was originally annotated as an independent ORF, but as a result of a sequence change, it was merged with an adjacent ORF into a single reading frame, designated YPR089W
S000006295	YPR091C	NVJ2	Nucleus-Vacuole Junction	716156	718468	-1	Lipid-binding ER protein, enriched at nucleus-vacuolar junctions (NVJ)	Lipid-binding ER protein, enriched at nucleus-vacuolar junctions (NVJ); involved in nonvesicular transfer of ceramides from ER to Golgi during ER stress; may be involved in sterol metabolism or signaling at the NVJ; contains a synaptotagmin-like-mitochondrial-lipid binding protein (SMP) domain; binds phosphatidylinositols and other lipids in a large-scale study; may interact with ribosomes, based on co-purification experiments
S000006296	YPR092W	""	""	718379	718684	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000006297	YPR093C	ASR1	Alcohol Sensitive Ring/PHD finger	719558	720424	-1	Ubiquitin ligase that modifies and regulates RNA Pol II	Ubiquitin ligase that modifies and regulates RNA Pol II; involved in a putative alcohol-responsive signaling pathway; accumulates in the nucleus under alcohol stress; has a role in organization of septins and the actin cytoskeleton; contains a Ring/PHD finger domain similar to the mammalian rA9 protein
S000006299	YPR095C	SYT1	Suppressor of ypt3	721037	724717	-1	Guanine nucleotide exchange factor (GEF) for Arf proteins	Guanine nucleotide exchange factor (GEF) for Arf proteins; promotes activation of Arl1p, which recruits Imh1p to the Golgi; involved in vesicular transport; member of the Sec7-domain family; contains a PH domain
S000006300	YPR096C	""	""	724842	725144	-1	Protein of unknown function	Protein of unknown function; may interact with ribosomes, based on co-purification experiments
S000006301	YPR097W	LEC1	Lipid-droplet Ergosterol Cortex	725394	728615	1	Phosphoinositide-binding regulator of ergosterol distribution	Phosphoinositide-binding regulator of ergosterol distribution; contains PX (Phox homology) domain; associated with ER-lipid droplet contact site; may facilitate ergosterol transport between plasma membrane and lipid droplets
S000006302	YPR098C	TMH18	TMem205 Homolog of 18 kDa	728947	729528	-1	Mitochondrial protein of unknown function	Mitochondrial protein of unknown function; localized to the mitochondrial outer membrane
S000006303	YPR099C	""	""	729757	730113	-1	Mitochondrial protein of unknown function	Mitochondrial protein of unknown function; null mutant has a respiratory growth defect and displays reduced peroxide-induced mitochondrial ROS; partially overlaps the verified gene MRPL51/YPR100W 
S000006304	YPR100W	MRPL51	Mitochondrial Ribosomal Protein, Large subunit	729791	730213	1	Mitochondrial ribosomal protein of the large subunit	Mitochondrial ribosomal protein of the large subunit; required for mitochondrial genome integrity, respiratory growth, and mitochondrial redox homeostasis, partially overlaps verified gene YPR099C
S000006305	YPR101W	SNT309	Synthetic lethal to prp NineTeen mutation	730492	731019	1	Member of the NineTeen Complex (NTC)	Member of the NineTeen Complex (NTC); this complex contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p
S000006307	YPR103W	PRE2	PRoteinase yscE	732349	733212	1	Beta 5 subunit of the 20S proteasome	Beta 5 subunit of the 20S proteasome; responsible for the chymotryptic activity of the proteasome; localizes to peroxisomes in oleate-growing cells
S000006308	YPR104C	FHL1	Fork Head-Like	733623	736433	-1	Regulator of ribosomal protein (RP) transcription	Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants
S000006309	YPR105C	COG4	Conserved Oligomeric Golgi complex	736984	739569	-1	Essential component of the conserved oligomeric Golgi complex	Essential component of the conserved oligomeric Golgi complex; a cytosolic tethering complex (Cog1p through Cog8p) that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
S000006310	YPR106W	ISR1	Inhibition of Staurosporine Resistance	740061	741392	1	Predicted protein kinase	Predicted protein kinase; negatively regulates the hexosamine biosynthetic pathway (HBP), which converts fructose-6-phosphate into UDP-N-acetylglucosamine, a precursor of chitin biosynthesis, GPI-anchor formation and glycosylation; interacts genetically with GFA1, encoding the first enzyme in the HBP; involved in Gfa1p phosphorylation (S332, T334); contains a Pho85p-regulated SCF-CDC4 phosphodegron; unstable, cell cycle regulated protein, peaking at G1/S
S000006311	YPR107C	YTH1	Yeast THirty kDa Homolog	741438	742064	-1	Essential RNA-binding component of cleavage and polyadenylation factor	Essential RNA-binding component of cleavage and polyadenylation factor; contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation; relocalizes to the cytosol in response to hypoxia
S000006312	YPR108W	RPN7	Regulatory Particle Non-ATPase	742454	743743	1	Essential non-ATPase regulatory subunit of the 26S proteasome	Essential non-ATPase regulatory subunit of the 26S proteasome; similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits
S000006313	YPR109W	GLD1	Golgi/endosome Localized Dsc protein	744689	745573	1	Component of Dsc E3 ligase complex in Golgi and endosomes	Component of Dsc E3 ligase complex in Golgi and endosomes; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum; diploid deletion strain has high budding index
S000006314	YPR110C	RPC40	RNA Polymerase C	745828	746835	-1	RNA polymerase subunit AC40	RNA polymerase subunit AC40; common to RNA polymerase I and III; predominant determinant targeting Ty1 integration upstream of Pol III-transcribed genes
S000006315	YPR111W	DBF20	DumbBell Forming	747306	749000	1	Ser/Thr kinase involved in late nuclear division	Ser/Thr kinase involved in late nuclear division; one of the mitotic exit network (MEN) proteins; necessary for the execution of cytokinesis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; DBF20 has a paralog, DBF2, that arose from the whole genome duplication
S000006316	YPR112C	MRD1	Multiple RNA-binding domain	749256	751919	-1	Essential conserved small ribosomal subunit (40s) synthesis factor	Essential conserved small ribosomal subunit (40s) synthesis factor; component of the 90S preribosome; required for production of 18S rRNA and small ribosomal subunit; contains five consensus RNA-binding domains and binds to the pre-rRNA at two sites within the 18S region
S000006317	YPR113W	PIS1	Phosphatidyl Inositol Synthase	752257	752919	1	Phosphatidylinositol synthase	Phosphatidylinositol synthase; required for biosynthesis of phosphatidylinositol, which is a precursor for polyphosphoinositides, sphingolipids, and glycolipid anchors for some of the plasma membrane proteins
S000006318	YPR114W	""	""	753301	754248	1	Protein distantly similar to ceramide synthases, Lag1p and Lac1p	Protein distantly similar to ceramide synthases, Lag1p and Lac1p; mutation confers sensitivity to inhibitors of sphingolipid biosynthesis
S000006319	YPR115W	RGC1	Regulator of the Glycerol Channel	754877	758128	1	Putative regulator of the Fps1p glycerol channel	Putative regulator of the Fps1p glycerol channel; multiply phosphorylated by Hog1p under osmotic stress; contains a pleckstrin homology domain; forms homodimers and heterodimerizes with paralog Ask10p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
S000006320	YPR116W	RRG8	Required for Respiratory Growth	758648	759481	1	Protein of unknown function	Protein of unknown function; required for efficient 5' processing of mitochondrial tRNAs, for respiratory growth and mitochondrial genome maintenance; null mutation results in a decrease in plasma membrane electron transport; localizes to the matrix side of the inner mitochondrial membrane
S000006321	YPR117W	HOB2	ortholog of Drosophila HOBbit	760025	767494	1	Modulator of cellular lipid distribution	Modulator of cellular lipid distribution; localizes to ER-PM and ER-mitochondria contact sites; shares homology with lipid transfer proteins Vps13p and Atg2p; homolog of D. melanogaster Hobbit protein
S000006322	YPR118W	MRI1	MethylthioRibose-1-phosphate Isomerase	767752	768987	1	5'-methylthioribose-1-phosphate isomerase	5'-methylthioribose-1-phosphate isomerase; catalyzes the isomerization of 5-methylthioribose-1-phosphate to 5-methylthioribulose-1-phosphate in the methionine salvage pathway
S000006323	YPR119W	CLB2	CycLin B	771653	773128	1	B-type cyclin involved in cell cycle progression	B-type cyclin involved in cell cycle progression; also involved in response to DNA damage; activates Cdc28p to promote the transition from G2 to M phase; accumulates during G2 and M, then targeted via a destruction box motif for ubiquitin-mediated degradation by the proteasome; CLB2 has a paralog, CLB1, that arose from the whole genome duplication
S000006324	YPR120C	CLB5	CycLin B	773875	775182	-1	B-type cyclin involved in DNA replication during S phase	B-type cyclin involved in DNA replication during S phase; activates Cdc28p to promote initiation of DNA synthesis; functions in formation of mitotic spindles along with Clb3p and Clb4p; most abundant during late G1 phase; CLB5 has a paralog, CLB6, that arose from the whole genome duplication
S000006325	YPR121W	THI22	THIamine metabolism	778583	780301	1	Protein with similarity to hydroxymethylpyrimidine phosphate kinases	Protein with similarity to hydroxymethylpyrimidine phosphate kinases; member of a gene family with THI20 and THI21; not required for thiamine biosynthesis; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum and vacuole respectively
S000006326	YPR122W	AXL1	AXiaL budding	782045	785671	1	Haploid specific endoprotease of a-factor mating pheromone	Haploid specific endoprotease of a-factor mating pheromone; performs one of two N-terminal cleavages during maturation of a-factor mating pheromone; required for axial budding pattern of haploid cells
S000006327	YPR123C	""	""	786141	786575	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially/completely overlaps the verified ORF CTR
S000006328	YPR124W	CTR1	Copper TRansport	786208	787428	1	High-affinity copper transporter of plasma membrane	High-affinity copper transporter of plasma membrane; mediates nearly all copper uptake under low copper conditions; transcriptionally induced at low copper levels and degraded at high copper levels; protein increases in abundance and relocalizes from nucleus to plasma membrane upon DNA replication stress; human homolog SLC31A1 can complement a yeast ctr1 ctr3 double deletion
S000006329	YPR125W	YLH47	Yeast LETM1 Homolog of 47 kD	787961	789325	1	Mitochondrial inner membrane protein	Mitochondrial inner membrane protein; exposed to the mitochondrial matrix; associates with mitochondrial ribosomes; NOT required for respiratory growth; homolog of human Letm1, a protein implicated in Wolf-Hirschhorn syndrome
S000006330	YPR126C	""	""	789077	789385	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000006331	YPR127W	""	""	790083	791120	1	Putative pyridoxine 4-dehydrogenase	Putative pyridoxine 4-dehydrogenase; differentially expressed during alcoholic fermentation; expression activated by transcription factor YRM1/YOR172W; green fluorescent protein (GFP)-fusion protein localizes to both the cytoplasm and the nucleus
S000006332	YPR128C	ANT1	Adenine Nucleotide Transporter	791218	792204	-1	Peroxisomal adenine nucleotide transporter	Peroxisomal adenine nucleotide transporter; involved in beta-oxidation of medium-chain fatty acid; required for peroxisome proliferation
S000006333	YPR129W	SCD6	Suppressor of Clathrin Deficiency	792687	793736	1	Repressor of translation initiation	Repressor of translation initiation; binds eIF4G through its RGG domain and inhibits recruitment of the preinitiation complex; also contains an Lsm domain; may have a role in RNA processing; overproduction suppresses null mutation in clathrin heavy chain gene CHC1; forms cytoplasmic foci upon DNA replication stress
S000006334	YPR130C	""	""	793374	793781	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000006335	YPR131C	NAT3	N-terminal AcetylTransferase	793911	794498	-1	Catalytic subunit of the NatB N-terminal acetyltransferase	Catalytic subunit of the NatB N-terminal acetyltransferase; NatB catalyzes acetylation of the amino-terminal methionine residues of all proteins beginning with Met-Asp or Met-Glu and of some proteins beginning with Met-Asn or Met-Met
S000006336	YPR132W	RPS23B	Ribosomal Protein of the Small subunit	794965	795767	1	Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit	Ribosomal protein 28 (rp28) of the small (40S) ribosomal subunit; required for translational accuracy; homologous to mammalian ribosomal protein S23 and bacterial S12; RPS23B has a paralog, RPS23A, that arose from the whole genome duplication; deletion of both RPS23A and RPS23B is lethal
S000006337	YPR133C	SPN1	Suppresses Postrecruitment functions gene Number 1	795978	797210	-1	Protein involved in RNA polymerase II transcription	Protein involved in RNA polymerase II transcription; also required for histone modifications and splicing; constitutively recruited to the CYC1 promoter and required for recruitment of chromatin remodeling factors for the expression of CYC1 gene; interacts genetically or physically with RNAP II, TBP, TFIIS, and chromatin remodelling factors; central domain is highly conserved throughout eukaryotes; mutations confer an Spt- phenotype
S000006338	YPR134W	MSS18	Mitochondrial Splicing System	798051	798857	1	Nuclear encoded protein needed for splicing of mitochondrial intron	Nuclear encoded protein needed for splicing of mitochondrial intron; required for efficient splicing of mitochondrial COX1 aI5beta intron; mss18 mutations block cleavage of 5' exon - intron junction; phenotype of intronless strain suggests additional functions
S000006339	YPR135W	CTF4	Chromosome Transmission Fidelity	799234	802017	1	Chromatin-associated protein	Chromatin-associated protein; required for sister chromatid cohesion; interacts with DNA polymerase alpha (Pol1p); recruits Mms22p to replication forks during S phase, and may link DNA synthesis to sister chromatid cohesion
S000006341	YPR137W	RRP9	Ribosomal RNA Processing	802359	804080	1	Protein involved in pre-rRNA processing	Protein involved in pre-rRNA processing; associated with U3 snRNP; component of small ribosomal subunit (SSU) processome; ortholog of the human U3-55k protein
S000006342	YPR138C	MEP3	""	810984	812453	-1	Ammonium permease of high capacity and low affinity	Ammonium permease of high capacity and low affinity; belongs to Mep-Amt-Rh family of well-conserved ammonium (NH4+) transporters that includes the human Rh factors; expression is under the nitrogen catabolite repression regulation ammonia permease activity regulated by TORC1 effectors, Npr1p and Par32p; MEP3 has a paralog, MEP1, that arose from the whole genome duplication
S000006343	YPR139C	LOA1	Lysophosphatidic acid: Oleoyl-CoA Acyltransferase	813156	814058	-1	Lysophosphatidic acid acyltransferase	Lysophosphatidic acid acyltransferase; involved in triacelglyceride homeostasis and lipid droplet formation; localized to lipid droplets and the ER; specificity for oleoyl-CoA
S000006344	YPR140W	TAZ1	TAfaZzin	814391	815536	1	Lyso-phosphatidylcholine acyltransferase	Lyso-phosphatidylcholine acyltransferase; required for normal phospholipid content of mitochondrial membranes; major determinant of the final acyl chain composition of the mitochondrial-specific phospholipid cardiolipin; mutations in human ortholog tafazzin (TAZ) cause Barth syndrome, a rare X-linked disease characterized by skeletal and cardiomyopathy and bouts of cyclic neutropenia; a specific splice variant of human TAZ can complement yeast null mutant
S000006345	YPR141C	KAR3	KARyogamy	815734	817923	-1	Minus-end-directed microtubule motor	Minus-end-directed microtubule motor; functions in mitosis and meiosis, localizes to the spindle pole body and localization is dependent on functional Cik1p, required for nuclear fusion during mating; potential Cdc28p substrate
S000006346	YPR142C	""	""	818130	818693	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RRP15, which is required for ribosomal RNA processing
S000006347	YPR143W	RRP15	Ribosomal RNA Processing	818323	819075	1	Nucleolar protein	Nucleolar protein; constituent of pre-60S ribosomal particles; required for proper processing of the 27S pre-rRNA at the A3 and B1 sites to yield mature 5.8S and 25S rRNAs
S000006348	YPR144C	NOC4	NucleOlar Complex associated	819765	821423	-1	Nucleolar protein	Nucleolar protein; forms a complex with Nop14p that mediates maturation and nuclear export of 40S ribosomal subunits; relocalizes to the cytosol in response to hypoxia
S000006349	YPR145W	ASN1	ASparagiNe requiring	822620	824338	1	Asparagine synthetase	Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN1 has a paralog, ASN2, that arose from the whole genome duplication
S000006350	YPR146C	""	""	825347	825676	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000006351	YPR147C	""	""	825645	826559	-1	Bifunctional triacylglycerol lipase and short chain ester hydrolase	Bifunctional triacylglycerol lipase and short chain ester hydrolase; null mutant results in the accumulation of both triacylglycerol and fatty acids derived from neutral lipids and phospholipids as well as an increase in the quantity of lipid droplets; contains an alpha/beta hydrolase domain with a conserved GXSXG lipase motif; localizes to lipid droplets; GFP-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS
S000006352	YPR148C	""	""	826833	828140	-1	Protein of unknown function	Protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
S000006353	YPR149W	NCE102	NonClassical Export	829918	830439	1	Protein involved in regulation of pheromone response and mating	Protein involved in regulation of pheromone response and mating; contains transmembrane domains; involved in secretion of proteins that lack classical secretory signal sequences; component of the detergent-insoluble glycolipid-enriched complexes (DIGs); NCE102 has a paralog, FHN1, that arose from the whole genome duplication
S000006354	YPR150W	""	""	830999	831520	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SUE1/YPR151C
S000006355	YPR151C	SUE1	""	831055	831675	-1	Protein required for degradation of unstable forms of cytochrome c	Protein required for degradation of unstable forms of cytochrome c; located in the mitochondria
S000006356	YPR152C	URN1	U2-U5-U6 snRNP, RES complex, and NTC interacting protein	832061	833458	-1	Protein of unknown function containing WW and FF domains	Protein of unknown function containing WW and FF domains; overexpression causes accumulation of cells in G1 phase
S000006357	YPR153W	MAY24	genetic interaction profile similarity to MTC Annotated Yeast genes MTC2 and MTC4	833689	834245	1	Peripheral ER membrane protein	Peripheral ER membrane protein; putative ER chaperone required for the stability and function of nutrient permeases exposed to high pressure
S000006358	YPR154W	PIN3	Psi+ INducibility	834565	835212	1	Negative regulator of actin nucleation-promoting factor activity	Negative regulator of actin nucleation-promoting factor activity; interacts with Las17p, a homolog of human Wiskott-Aldrich Syndrome protein (WASP), via an N-terminal SH3 domain, and along with LSB1 cooperatively inhibits the nucleation of actin filaments; short-lived protein whose levels increase in response to thermal stress; induces the formation of the [PIN+] and [RNQ+] prions when overproduced; PIN3 has a paralog, LSB1, that arose from the whole genome duplication
S000006359	YPR155C	NCA2	Nuclear Control of ATPase	835563	837413	-1	Protein that regulates expression of Fo-F1 ATP synthase subunits	Protein that regulates expression of Fo-F1 ATP synthase subunits; involved in the regulation of mitochondrial expression of subunits 6 (Atp6p) and 8 (Atp8p) of the Fo-F1 ATP synthase; functions with Nca3p
S000006360	YPR156C	TPO3	Transporter of POlyamines	837909	839777	-1	Polyamine transporter of major facilitator superfamily	Polyamine transporter of major facilitator superfamily; member of 12-spanner drug:H(+) antiporter DHA1 family; specific for spermine; localizes to plasma membrane; targeted to vacuole via AP-3 pathway; TPO3 has a paralog, TPO2, that arose from the whole genome duplication
S000006361	YPR157W	TDA6	Topoisomerase I Damage Affected	841266	842669	1	Putative protein of unknown function	Putative protein of unknown function; induced by treatment with 8-methoxypsoralen and UVA irradiation; null mutant is sensitive to expression of the top1-T722A allele; SWAT-GFP and mCherry fusion proteins localize to the cell periphery and vacuole; TDA6 has a paralog, VPS62, that arose from the whole genome duplication
S000006362	YPR158W	CUR1	Curing of [URe3]	843262	844020	1	Sorting factor, central regulator of spatial protein quality control	Sorting factor, central regulator of spatial protein quality control; physically and functionally interacts with chaperones to promote sorting and deposition of misfolded proteins into cytosolic compartments; involved in destabilization of [URE3] prions; CUR1 has a paralog, BTN2, that arose from the whole genome duplication
S000006363	YPR159W	KRE6	Killer toxin REsistant	857583	859745	1	Glucosyl hydrolase required for beta-1,6-glucan biosynthesis	Glucosyl hydrolase required for beta-1,6-glucan biosynthesis; type II integral membrane protein that localizes to ER, plasma membrane, sites of polarized growth and secretory vesicles; functionally redundant with Skn1p; KRE6 has a paralog, SKN1, that arose from the whole genome duplication
S000006364	YPR160W	GPH1	Glycogen PHosphorylase	861306	864014	1	Glycogen phosphorylase required for the mobilization of glycogen	Glycogen phosphorylase required for the mobilization of glycogen; non-essential; regulated by cyclic AMP-mediated phosphorylation; phosphorylation by Cdc28p may coordinately regulate carbohydrate metabolism and the cell cycle; expression is regulated by stress-response elements and by the HOG MAP kinase pathway
S000006365	YPR161C	SGV1	Suppressor of Gpa1-Val50 mutation	864449	866422	-1	Cyclin (Bur2p)-dependent protein kinase	Cyclin (Bur2p)-dependent protein kinase; subunit of the CDK-cyclin BUR kinase complex with Bur2p; regulates Set2p-dependent H3K36 trimethylation; regulates transcriptional elongation through the phosphorylation of the carboxy-terminal domain (CTD) of Rpo21p; stimulates recruitment of the PAF complex through Spt5p CTD phosphorylation; recruits Spt6p to the CTD at the onset of transcription; regulates telomere elongation; regulated by Cak1p; similar to metazoan CDK9 proteins
S000006367	YPR163C	TIF3	Translation Initiation Factor	868645	869955	-1	Translation initiation factor eIF-4B	Translation initiation factor eIF-4B; RNA recognition motif containing single-stranded RNA binding protein that possesses RNA annealing and strand-exchange activities; stimulates the ATPase and unwinding activities of the RNA helicase eIF4A, in a synergistic manner with eIF4G; promotes assembly of the translation initiation factor complex eIF4F and the 48S preinitiation complex; interacts with Rps20p at the head of the 40S ribosomal subunit and alters the structure of the mRNA entry channel 
S000006368	YPR164W	MMS1	Methyl MethaneSulfonate sensitivity	870703	874926	1	Subunit of E3 ubiquitin ligase complex involved in replication repair	Subunit of E3 ubiquitin ligase complex involved in replication repair; stabilizes protein components of the replication fork such as the fork-pausing complex and leading strand polymerase, preventing fork collapse and promoting efficient recovery during replication stress; regulates Ty1 transposition; involved with Rtt101p in nonfunctional rRNA decay
S000006370	YPR166C	MRP2	Mitochondrial Ribosomal Protein	876282	876629	-1	Mitochondrial ribosomal protein of the small subunit	Mitochondrial ribosomal protein of the small subunit
S000006371	YPR167C	MET16	METhionine requiring	876847	877632	-1	3'-phosphoadenylsulfate reductase	3'-phosphoadenylsulfate reductase; reduces 3'-phosphoadenylyl sulfate to adenosine-3',5'-bisphosphate and free sulfite using reduced thioredoxin as cosubstrate, involved in sulfate assimilation and methionine metabolism
S000006372	YPR168W	NUT2	Negative regulation of URS Two	878076	878549	1	Subunit of the RNA polymerase II mediator complex	Subunit of the RNA polymerase II mediator complex; conserved from yeast to human; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; required for transcriptional activation and has a role in basal transcription; protein abundance increases in response to DNA replication stress
S000006373	YPR169W	JIP5	Jumonji domain Interacting Protein	878690	880168	1	Protein required for biogenesis of the large ribosomal subunit	Protein required for biogenesis of the large ribosomal subunit; required for biogenesis of the large ribosomal subunit; interacts with proteins involved in RNA processing, ribosome biogenesis, ubiquitination and demethylation; similar to WDR55, a human WD repeat protein; essential gene
S000006374	YPR170C	""	""	882983	883318	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps ORFs YPR169W-A and YPR170W-B
S000006375	YPR171W	BSP1	Binding protein of Synaptojanin Polyphosphoinositide phosphatase domain	883828	885558	1	Adapter that links synaptojanins to the cortical actin cytoskeleton	Adapter that links synaptojanins to the cortical actin cytoskeleton; the synaptojanins are Inp52p and Inp53p 
S000006376	YPR172W	""	""	885796	886398	1	Protein of unknown function	Protein of unknown function; predicted to encode a pyridoxal 5'-phosphate synthase based on sequence similarity but purified protein does not possess this activity, nor does it bind flavin mononucleotide (FMN); transcriptionally activated by Yrm1p along with genes involved in multidrug resistance; YPR172W has a paralog, YLR456W, that arose from the whole genome duplication
S000006377	YPR173C	VPS4	Vacuolar Protein Sorting	886524	887837	-1	AAA-ATPase involved in multivesicular body (MVB) protein sorting	AAA-ATPase involved in multivesicular body (MVB) protein sorting; ATP-bound Vps4p localizes to endosomes and catalyzes ESCRT-III disassembly and membrane release; ATPase activity is activated by Vta1p; regulates cellular sterol metabolism
S000006378	YPR174C	CSA1	Cdc5 SPB Anchor	888043	888708	-1	Protein of unknown function	Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nuclear periphery; potential Cdc28p substrate; binds phosphatidylinositols and phosphatidylethanolamine in a large-scale study; relative distribution to foci at the nuclear periphery increases upon DNA replication stress; YPR174C has a paralog, NBP1, that arose from the whole genome duplication
S000006379	YPR175W	DPB2	DNA Polymerase B	888974	891043	1	Second largest subunit of DNA polymerase II (DNA polymerase epsilon)	Second largest subunit of DNA polymerase II (DNA polymerase epsilon); required for maintenance of fidelity of chromosomal replication; essential motif in C-terminus is required for formation of the four-subunit Pol epsilon; expression peaks at the G1/S phase boundary; Cdc28p substrate
S000006380	YPR176C	BET2	Blocked Early in Transport	891101	892078	-1	Beta subunit of Type II geranylgeranyltransferase	Beta subunit of Type II geranylgeranyltransferase; required for vesicular transport between the endoplasmic reticulum and the Golgi; provides a membrane attachment moiety to Rab-like proteins Ypt1p and Sec4p; human homolog RABGGTB can complement yeast BET2 mutant
S000006382	YPR178W	PRP4	Pre-mRNA Processing	892332	893729	1	Splicing factor	Splicing factor; component of the U4/U6-U5 snRNP complex
S000006383	YPR179C	HDA3	Histone DeAcetylase	893797	895764	-1	Subunit of HDA1 histone deacetylase complex	Subunit of HDA1 histone deacetylase complex; tetrameric trichostatin A-sensitive class II histone deacetylase complex contains Hda1p homodimer and an Hda2p-Hda3p heterodimer; required for activity of complex; relocalizes to cytosol in response to hypoxia; similar to Hda2p; the HDA1 histone deacetylase complex regulates aging through trehalose metabolism
S000006384	YPR180W	AOS1	Activation Of Smt3p	895961	897004	1	Subunit of heterodimeric nuclear SUMO activating enzyme E1 with Uba2p	Subunit of heterodimeric nuclear SUMO activating enzyme E1 with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability; relocalizes to the cytosol in response to hypoxia
S000006387	YPR183W	DPM1	Dolichol Phosphate Mannose synthase	900755	901558	1	Dolichol phosphate mannose (Dol-P-Man) synthase of ER membrane	Dolichol phosphate mannose (Dol-P-Man) synthase of ER membrane; catalyzes formation of Dol-P-Man from Dol-P and GDP-Man; required for biosynthesis of glycosyl phosphatidylinositol (GPI) membrane anchor, as well as O-mannosylation and protein N- and O-linked glycosylation; human homolog DPM1 can complement yeast mutant strains
S000006388	YPR184W	GDB1	Glycogen DeBranching	902044	906654	1	Glycogen debranching enzyme	Glycogen debranching enzyme; contains glucanotranferase and alpha-1,6-amyloglucosidase activities; required for glycogen degradation; phosphorylated in mitochondria; activity is inhibited by Igd1p; protein abundance increases in response to DNA replication stress
S000006389	YPR185W	ATG13	AuTophaGy related	907218	909434	1	Regulatory subunit of the Atg1p signaling complex	Regulatory subunit of the Atg1p signaling complex; stimulates Atg1p kinase activity; required for vesicle formation during autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; contains a HORMA domain required for autophagy and for recruitment of the phosphatidylinositol 3-kinase complex subunit Atg14p to the pre-autophagosomal structure
S000006390	YPR186C	PZF1	Putative Zinc Finger	909733	911022	-1	Transcription factor IIIA (TFIIIA)	Transcription factor IIIA (TFIIIA); essential DNA binding protein required for transcription of 5S rRNA by RNA polymerase III; not involved in transcription of other RNAP III genes; nine conserved zinc fingers; may also bind 5S rRNA
S000006392	YPR188C	MLC2	Myo1p Light Chain	911988	912479	-1	Regulatory light chain for the type II myosin Myo1p	Regulatory light chain for the type II myosin Myo1p; binds to an IQ motif of Myo1p, localization to the bud neck depends on Myo1p; involved in the disassembly of the Myo1p ring
S000006393	YPR189W	SKI3	SuperKIller	912664	916962	1	Ski complex component and TPR protein	Ski complex component and TPR protein; mediates 3'-5' RNA degradation by the cytoplasmic exosome; null mutants have superkiller phenotype of increased viral dsRNAs and are synthetic lethal with mutations in 5'-3' mRNA decay; role in preventing L-A mycovirus pathogenesis; mutations in the human ortholog, TTC37, causes Syndromic diarrhea/Trichohepatoenteric (SD/THE) syndrome
S000006394	YPR190C	RPC82	RNA Polymerase C	917077	919041	-1	RNA polymerase III subunit C82	RNA polymerase III subunit C82
S000006395	YPR191W	QCR2	QH2:cytochrome-C oxidoReductase	919381	920487	1	Subunit 2 of ubiquinol cytochrome-c reductase (Complex III)	Subunit 2 of ubiquinol cytochrome-c reductase (Complex III); Complex III is a component of the mitochondrial inner membrane electron transport chain; phosphorylated; transcription is regulated by Hap1p, Hap2p/Hap3p, and heme
S000006396	YPR192W	AQY1	AQuaporin from Yeast	921860	922777	1	Spore-specific water channel	Spore-specific water channel; mediates the transport of water across cell membranes, developmentally controlled; may play a role in spore maturation, probably by allowing water outflow, may be involved in freeze tolerance
S000006397	YPR193C	HPA2	Histone and other Protein Acetyltransferase	922909	923379	-1	Tetrameric histone acetyltransferase	Tetrameric histone acetyltransferase; has similarity to Gcn5p, Hat1p, Elp3p, and Hpa3p; acetylates histones H3 and H4 in vitro and exhibits autoacetylation activity; also acetylates polyamines
S000006398	YPR194C	OPT2	OligoPeptide Transporter	924304	926937	-1	Oligopeptide transporter	Oligopeptide transporter; localized to peroxisomes and affects glutathione redox homeostasis; also localizes to the plasma membrane (PM) and to the late Golgi, and has a role in maintenance of lipid asymmetry between the inner and outer leaflets of the PM; member of the OPT family, with potential orthologs in S. pombe and C. albicans; also plays a role in formation of mature vacuoles and in polarized cell growth
S000006399	YPR195C	""	""	927965	928294	-1	Putative protein of unknown function	Putative protein of unknown function; conserved across S. cerevisiae strains
S000006400	YPR196W	""	""	931376	932788	1	Putative maltose-responsive transcription factor	Putative maltose-responsive transcription factor
S000006401	YPR197C	""	""	933898	934461	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF SGE1/YPR198W
S000006402	YPR198W	SGE1	Suppression of Gal11 Expression	934034	935665	1	Plasma membrane multidrug transporter	Plasma membrane multidrug transporter; member of the major facilitator superfamily; acts as an extrusion permease; partial multicopy suppressor of gal11 mutations
S000006403	YPR199C	ARR1	ARsenicals Resistance	938148	939032	-1	Transcriptional activator of the basic leucine zipper (bZIP) family	Transcriptional activator of the basic leucine zipper (bZIP) family; required for transcription of genes involved in resistance to arsenic compounds; directly binds trivalent arsenic (As(III)) as does K. lactis ortholog, KIYAP8
S000006404	YPR200C	ARR2	ARsenicals Resistance	939279	939671	-1	Arsenate reductase required for arsenate resistance	Arsenate reductase required for arsenate resistance; converts arsenate to arsenite which can then be exported from cells by Arr3p
S000006405	YPR201W	ARR3	ARsenicals Resistance	939922	941136	1	Plasma membrane metalloid/H+ antiporter	Plasma membrane metalloid/H+ antiporter; member of bile/arsenite/riboflavin transporter (BART) superfamily; transports arsenite and antimonite; required for resistance to arsenic compounds; transcription is activated by Arr1p in the presence of arsenite; protein displays 10 transmembrane segments with cytoplasmically oriented N- and C-terminal domains
S000006429	YLR390W-A	CCW14	Covalently linked Cell Wall protein	903724	904440	1	Covalently linked cell wall glycoprotein	Covalently linked cell wall glycoprotein; present in the inner layer of the cell wall
S000006430	YDL045W-A	MRP10	Mitochondrial Ribosomal Protein	372248	372535	1	Mitochondrial ribosomal protein of the small subunit	Mitochondrial ribosomal protein of the small subunit; contains twin cysteine-x9-cysteine motifs; oxidized by Mia40p during import into mitochondria
S000006431	YOR008C-A	""	""	342857	343081	-1	Putative protein of unknown function	Putative protein of unknown function; includes a potential transmembrane domain; deletion results in slightly lengthened telomeres; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum and vacuole respectively
S000006432	YIL009C-A	EST3	Ever Shorter Telomeres	335666	336212	-1	Component of the telomerase holoenzyme	Component of the telomerase holoenzyme; involved in telomere replication; synthesis of the full-length protein results from a programmed +1 ribosomal frameshift
S000006433	YPR133W-A	TOM5	Translocase of the Outer Mitochondrial membrane	797557	797709	1	Component of the TOM (translocase of outer membrane) complex	Component of the TOM (translocase of outer membrane) complex; responsible for recognition and initial import of all mitochondrially directed proteins; involved in transfer of precursors from the Tom70p and Tom20p receptors to the Tom40p pore
S000006434	YLR438C-A	LSM3	Like SM	1013909	1014178	-1	Lsm (Like Sm) protein	Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
S000006435	YKR035W-A	DID2	Doa4-Independent Degradation	507939	508553	1	Class E protein of the vacuolar protein-sorting (Vps) pathway	Class E protein of the vacuolar protein-sorting (Vps) pathway; binds Vps4p and directs it to dissociate ESCRT-III complexes; forms a functional and physical complex with Ist1p; human ortholog may be altered in breast tumors
S000006436	YFL034C-A	RPL22B	Ribosomal Protein of the Large subunit	64243	64932	-1	Ribosomal 60S subunit protein L22A	Ribosomal 60S subunit protein L22A; required for translation of long 5' UTR of IME1 mRNA and meiotic entry; autoregulates splicing of its pre-m-RNA; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22B has a paralog, RPL22A, that arose from the whole genome duplication
S000006437	YFR032C-A	RPL29	Ribosomal Protein of the Large subunit	223258	223437	-1	Ribosomal 60S subunit protein L29	Ribosomal 60S subunit protein L29; not essential for translation, but required for proper joining of large and small ribosomal subunits and for normal translation rate; homologous to mammalian ribosomal protein L29, no bacterial homolog
S000006438	YPL249C-A	RPL36B	Ribosomal Protein of the Large subunit	75699	76239	-1	Ribosomal 60S subunit protein L36B	Ribosomal 60S subunit protein L36B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L36, no bacterial homolog; RPL36B has a paralog, RPL36A, that arose from the whole genome duplication
S000006439	YCR020W-B	HTL1	High-Temperature Lethal	155320	155556	1	Component of the RSC chromatin remodeling complex	Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
S000007221	YCL001W-A	""	""	113080	113541	1	Putative protein of unknown function	Putative protein of unknown function; YCL001W-A gene has similarity to DOM34 and is present in a region duplicated between chromosomes XIV and III
S000007222	YCR028C-A	RIM1	Replication In Mitochondria	172950	173440	-1	ssDNA-binding protein essential for mitochondrial genome maintenance	ssDNA-binding protein essential for mitochondrial genome maintenance; involved in mitochondrial DNA replication; stimulates utilization by Mip1p DNA polymerase of RNA primers synthesized by Rpo41p
S000007223	YCR087C-A	""	""	264006	264467	-1	Putative protein of unknown function	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus; YCR087C-A is not an essential gene
S000007224	YPL183W-A	RTC6	Restriction of Telomere Capping	199095	199376	1	Protein involved in translation	Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress
S000007226	YER039C-A	""	""	229263	229481	-1	Putative protein of unknown function	Putative protein of unknown function; YER039C-A is not an essential gene
S000007228	YBL091C-A	SCS22	Suppressor of Choline Sensitivity	46565	47180	-1	Protein involved in regulation of phospholipid metabolism	Protein involved in regulation of phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect the ER and plasma membrane (PM); regulates PM PI4P levels by controlling access of the Sac1p phosphatase to its substrate, PI4P; human VAP homolog; similar to D. melanogaster inturned protein; SWAT-GFP and mCherry fusion proteins localize to the cytosol; SCS22 has a paralog, SCS2, that arose from the whole genome duplication
S000007231	YCR102W-A	""	""	306735	306932	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000007232	YDL130W-A	STF1	STabilizing Factor	229171	229431	1	Protein involved in regulation of the mitochondrial F1F0-ATP synthase	Protein involved in regulation of the mitochondrial F1F0-ATP synthase; Stf1p and Stf2p act as stabilizing factors that enhance inhibitory action of the Inh1p protein; protein abundance increases in response to DNA replication stress; STF1 has a paralog, INH1, that arose from the whole genome duplication
S000007234	YDR034W-B	""	""	521314	521469	1	Predicted tail-anchored plasma membrane protein	Predicted tail-anchored plasma membrane protein; upregulated by toxic concentrations of heavy metal ions including Mn2+, Co2+, Ni2+, Cu2+, Zn2+, Cd2+ and alkali; localizes to the cytoplasm, possibly in intracellular membranes; CYSTM (cysteine-rich transmembrane module) family member; may be involved in response to stress; N- and C-terminal fusion proteins localize to the cell periphery; YDR034W-B has a paralog, YBR056W-A, that arose from the whole genome duplication
S000007235	YDR363W-A	SEM1	Suppressor of Exocyst Mutations	1202128	1202397	1	19S proteasome regulatory particle lid subcomplex component	19S proteasome regulatory particle lid subcomplex component; role in Ub-dependent proteolysis and proteasome stability; involved in TREX-2 mediated mRNA export, and in the prevention of transcription-associated genome instability; ubiquitinated by Nedd4-like E3-ligase, Rsp5p; human ortholog DSS1, a BRCA1 binding protein implicated in cancer, complements the yeast null; drives trinucleotide repeat expansion; protein abundance increases in response to DNA replication stress
S000007236	YDR525W-A	SNA2	Sensitivity to NA+	1490596	1490835	1	Protein of unknown function	Protein of unknown function; has similarity to Pmp3p, which is involved in cation transport; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
S000007237	YER048W-A	ISD11	Iron-Sulfur protein biogenesis, Desulfurase-interacting protein	250718	251002	1	Cysteine desulfurase (Nfs1p) activator	Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis; exclusive to eukaryotes, implicated as eukaryotic supplement to the bacterium-derived Fe-S cluster (ISC) assembly apparatus; involved in regulation of iron metabolism; member of the LYR protein family
S000007238	YER091C-A	""	""	342390	342611	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000007240	YGR122C-A	""	""	735630	735758	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; similar to YLR334C and YOL106W
S000007241	YIR020W-A	""	""	394917	395159	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000007242	YKL033W-A	""	""	374505	375215	1	Protein of unknown function	Protein of unknown function; similar to uncharacterized proteins from other fungi
S000007243	YKL053C-A	MDM35	Mitochondrial Distribution and Morphology	339182	339442	-1	Mitochondrial intermembrane space protein	Mitochondrial intermembrane space protein; forms complex with Ups2p that transfers phosphatidylserine from outer membrane to inner membrane for phosphatidylethanolamine synthesis; mutation affects mitochondrial distribution and morphology; contains twin cysteine-x9-cysteine motifs; protein abundance increases in response to DNA replication stress
S000007244	YKL162C-A	""	""	145922	146074	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000007245	YLL018C-A	COX19	Cytochrome c OXidase	108676	108972	-1	Protein required for cytochrome c oxidase assembly	Protein required for cytochrome c oxidase assembly; located in the cytosol and mitochondrial intermembrane space; putative copper metallochaperone that delivers copper to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs
S000007246	YLR262C-A	TMA7	Translation Machinery Associated	669468	669662	-1	Protein of unknown function that associates with ribosomes	Protein of unknown function that associates with ribosomes; null mutant exhibits translation defects, altered polyribosome profiles, and resistance to the translation inhibitor anisomcyin; protein abundance increases in response to DNA replication stress
S000007247	YML081C-A	ATP18	ATP synthase	103983	104162	-1	Subunit of the mitochondrial F1F0 ATP synthase	Subunit of the mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; termed subunit I or subunit j; does not correspond to known ATP synthase subunits in other organisms
S000007249	YMR158C-A	""	""	573194	573331	-1	Protein of unknown function	Protein of unknown function; may contain a lipid attachment site; localizes to cytosol, and to peroxisomes in oleate-growing cells; YMR158C-A is not an essential gene
S000007250	YMR194C-A	""	""	652912	653136	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000007251	YNR032C-A	HUB1	Homologous to UBiquitin	687243	687464	-1	Ubiquitin-like protein modifier	Ubiquitin-like protein modifier; promotes alternative splicing of SRC1 pre-mRNA; binds non-covalently to the HIND domain of Snu66, may function in modification of Sph1p and Hbt1p, functionally complemented by the human or S. pombe ortholog; mechanism of Hub1p adduct formation not yet clear
S000007252	YOL013W-B	""	""	300690	300980	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the long terminal repeat (LTR) of a Ty1 element
S000007253	YOR298C-A	MBF1	Multiprotein Bridging Factor	877230	877685	-1	Transcriptional coactivator	Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress
S000007255	YDR322C-A	TIM11	Translocase of the Inner Mitochondrial membrane	1112003	1112293	-1	Subunit e of mitochondrial F1F0-ATPase	Subunit e of mitochondrial F1F0-ATPase; ATPase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; essential for the dimeric and oligomeric state of ATP synthase, which in turn determines the shape of inner membrane cristae
S000007339	YOL077W-A	ATP19	ATP synthase	185438	185644	1	Subunit k of the mitochondrial F1F0 ATP synthase	Subunit k of the mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; associated only with the dimeric form of ATP synthase
S000007348	YJR135W-A	TIM8	Translocase of the Inner Mitochondrial membrane	676971	677234	1	Mitochondrial intermembrane space protein	Mitochondrial intermembrane space protein; forms a complex with Tim13p that delivers a subset of hydrophobic proteins to the TIM22 complex for inner membrane insertion; homolog of human TIMM8A, implicated in Mohr-Tranebjaerg syndrome, also known as deafness-dystonia-optic neuronopathy (DDON) syndrome; human TIMM8A can complement yeast null mutant
S000007427	YER186W-A	""	""	565667	566260	1	Deleted ORF	Deleted ORF; does not encode a protein; the YER186W-A was removed because it does not begin with a start codon or end with a stop codon, and has an in-frame internal stop codon (see the Locus History for more details)
S000007472	YML058W-A	HUG1	""	158760	158966	1	Ribonucleotide reductase inhibitor	Ribonucleotide reductase inhibitor; intrinsically disordered protein that binds to and inhibits Rnr2p; involved in the Mec1p-mediated checkpoint pathway; transcription is induced by genotoxic stress and by activation of the Rad53p pathway; protein abundance increases in response to DNA replication stress
S000007496	YHR132W-A	IGO2	Initiation of G zerO	370054	370449	1	Protein required for initiation of G0 program	Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; IGO2 has a paralog, IGO1, that arose from the whole genome duplication
S000007521	YBR058C-A	TSC3	Temperature-sensitive Suppressors of Csg2 mutants	356324	356566	-1	Protein that stimulates the activity of serine palmitoyltransferase	Protein that stimulates the activity of serine palmitoyltransferase;  involved in sphingolipid biosynthesis; Lcb1p and Lcb2p are the two components of serine palmitoyltransferase
S000007522	YBR085C-A	""	""	418907	419164	-1	Protein of unknown function	Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and to the nucleus; protein abundance increases in response to DNA replication stress
S000007523	YER053C-A	""	""	260933	261046	-1	Protein of unknown function	Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress
S000007547	YCL057C-A	MIC10	MItochondrial contact site and Cristae organizing system	24032	24325	-1	Conserved component of the MICOS complex	Conserved component of the MICOS complex; MICOS (formerly MINOS or MitOS) is a mitochondrial inner membrane complex that extends into the intermembrane space and has a role in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane; forms a subcomplex with Mic12p and Mic27p whose assembly and stability requires cardiolipin; homo-oligomers cause membrane bending; ortholog of human MINOS1
S000007548	YCL026C-B	HBN1	Homologous to Bacterial Nitroreductases	73405	73986	-1	Protein of unknown function	Protein of unknown function; similar to bacterial nitroreductases; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein becomes insoluble upon intracellular iron depletion; protein abundance increases in response to DNA replication stress
S000007549	YCL021W-A	""	""	83620	83997	1	Putative protein of unknown function	Putative protein of unknown function; SWAT-GFP and mCherry fusion proteins localize to the vacuole
S000007586	YAL044W-A	BOL1	BolA-like protein	57518	57850	1	Mitochondrial matrix protein involved in Fe-S cluster biogenesis	Mitochondrial matrix protein involved in Fe-S cluster biogenesis; facilitates [4Fe-2S] cluster inception into mitochondrial proteins such as lipoate synthase and succinate dehydrogenase; interacts and may function with Grx5p at an early step in Fe-S cluster biosynthesis; forms dimeric complexes with Grx5p and Nfu1p that alter the stability of shared Fe/S clusters; sequence similarity to human BOLA family member, BOLA1 and S. pombe uvi31, a putative DNA repair protein
S000007587	YBL071W-A	KTI11	Kluveromyces lactis Toxin Insensitive	89978	90226	1	Zn-ribbon protein that co-purifies with Dph1 and Dph2	Zn-ribbon protein that co-purifies with Dph1 and Dph2; forms a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p; involved in modification of wobble nucleosides in tRNAs; forms a stable heterodimer with Ats1p
S000007588	YDL085C-A	""	""	302464	302670	-1	Homolog of modifier of aggregation-4 (MOAG-4) from worm and small EDRK	Homolog of modifier of aggregation-4 (MOAG-4) from worm and small EDRK-rich factor protein (SERF1a) from human; highly conserved enhancer of amyloid formation of Aβ40 and α-synuclein both in vitro and in vivo; protein displays very high degree of disorder, segment spanning residues 36E-65K has strong helical propensity
S000007590	YER014C-A	BUD25	BUD site selection	183731	184700	-1	Protein involved in bud-site selection	Protein involved in bud-site selection; diploid mutants display a random budding pattern instead of the wild-type bipolar pattern
S000007591	YBL029C-A	""	""	164488	164772	-1	Protein of unknown function	Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress; has potential orthologs in Saccharomyces species and in Yarrowia lipolytica
S000007596	YCL001W-B	""	""	113770	114024	1	Putative protein of unknown function	Putative protein of unknown function; present in a region duplicated between chromosomes XIV and III; YCL001W-B has a paralog, DOM34, that arose from the whole genome duplication
S000007599	YDL159W-A	""	""	172182	172313	1	Putative protein of unknown function	Putative protein of unknown function; identified by sequence comparison with hemiascomycetous yeast species
S000007603	YDR079C-A	TFB5	""	603593	603811	-1	Component of RNA polymerase II general transcription factor TFIIH	Component of RNA polymerase II general transcription factor TFIIH; involved in transcription initiation and in nucleotide-excision repair; relocalizes to the cytosol in response to hypoxia; homolog of Chlamydomonas reinhardtii REX1-S protein involved in DNA repair
S000007605	YDR379C-A	SDH6	""	1233278	1233517	-1	Mitochondrial protein involved in assembly of succinate dehydrogenase	Mitochondrial protein involved in assembly of succinate dehydrogenase; has a role in maturation of the Sdh2p subunit; member of the LYR protein family; mutations in human ortholog SDHAF1 are associated with infantile leukoencephalopathy
S000007606	YFR012W-A	""	""	169224	169310	1	Putative protein of unknown function	Putative protein of unknown function; identified by homology
S000007608	YGR271C-A	EFG1	Exit From G1	1037800	1038501	-1	Ribosome biogenesis factor required for maturation of 18S rRNA	Ribosome biogenesis factor required for maturation of 18S rRNA; null mutant is sensitive to hydroxyurea and is delayed in recovering from alpha-factor arrest; green fluorescent protein (GFP)-fusion protein localizes to the nucleolus
S000007609	YJL012C-A	""	""	410930	411127	-1	Merged open reading frame	Merged open reading frame; does not encode a discrete protein; YJL012C-A was originally annotated as an independent ORF but due to a sequence change was merged with an adjacent ORF into a single reading frame, designated YJL012C/VTC4
S000007611	YJL062W-A	COA3	Cytochrome Oxidase Assembly	316723	316980	1	Mitochondrial protein required for cytochrome c oxidase assembly	Mitochondrial protein required for cytochrome c oxidase assembly; also involved in translational regulation of Cox1p and prevention of Cox1p aggregation before assembly; located in the mitochondrial inner membrane
S000007615	YKL018C-A	MCO12	Mitochondrial Class One protein of 12 kDa	403580	403879	-1	Putative mitochondrial protein of unknown function	Putative mitochondrial protein of unknown function; identified by homology; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
S000007616	YKL106C-A	""	""	237147	237266	-1	Putative protein of unknown function	Putative protein of unknown function; identified by homology to uncharacterized proteins in other fungi
S000007620	YLR363W-A	""	""	853461	853718	1	Protein of unknown function	Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; relocalizes from nucleus to nucleolus upon DNA replication stress
S000007621	YML007C-A	MIN4	mitochondrial MINi protein of 4 kDa	253162	253272	-1	Mitochondrial protein of unknown function	Mitochondrial protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria
S000007622	YMR013W-A	""	""	298311	298391	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; completely overlaps the characterized snoRNA gene snR73
S000007624	YNL162W-A	""	""	330329	330547	1	Putative protein of unknown function	Putative protein of unknown function; identified by homology
S000007626	YOL086W-A	MHF1	Mph1-associated Histone-Fold protein	159173	159445	1	Component of the heterotetrameric MHF histone-fold complex	Component of the heterotetrameric MHF histone-fold complex; in humans the MHF complex interacts with both DNA and Mph1p ortholog FANCM, a Fanconi anemia complementation group protein, to stabilize and remodel blocked replication forks and repair damaged DNA; mhf1 srs2 double mutants are MMS hypersensitive; ortholog of human centromere constitutive-associated network (CCAN) subunit CENP-S, also known as MHF1
S000007627	YOL159C-A	""	""	15233	15505	-1	Protein of unknown function	Protein of unknown function; overexpression affects endocytic protein trafficking; identified by sequence comparison with hemiascomycetous yeast species
S000028423	YPL096C-A	ERI1	ER-associated Ras Inhibitor	366529	366735	-1	Endoplasmic reticulum membrane protein that binds and inhibits Ras2p	Endoplasmic reticulum membrane protein that binds and inhibits Ras2p; binds to and inhibits GTP-bound Ras2p at the endoplasmic reticulum (ER); component of the GPI-GnT complex which catalyzes the first step in GPI-anchor biosynthesis; probable homolog of mammalian PIG-Y protein
S000028437	YER180C-A	SLO1	Scoco-Like Orf	550865	551122	-1	Protein interacting with Arl3p	Protein interacting with Arl3p; Arl3p is a GTPase of the Ras superfamily involved in vesicle-tethering at the Golgi; putative ortholog of human SCOCO
S000028508	YCL005W-A	VMA9	Vacuolar Membrane Atpase	107023	107417	1	Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex	Vacuolar H+ ATPase subunit e of the V-ATPase V0 subcomplex; essential for vacuolar acidification; interacts with the V-ATPase assembly factor Vma21p in the ER; involved in V0 biogenesis
S000028510	YBR111W-A	SUS1	Sl gene Upstream of ySa1	462139	462579	1	Global transcriptional regulator, component of SAGA, TREX-2 complexes	Global transcriptional regulator, component of SAGA, TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to nuclear periphery and to non-nascent mRNP; plays role in negatively regulating telomere length through modulation of H2BK123 mono-ubiquitination and its interaction with nuclear pore complex
S000028513	YJR112W-A	""	""	637790	638168	1	Putative protein of unknown function	Putative protein of unknown function; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum; identified based on homology to <i>Ashbya gossypii</i>
S000028514	YMR194C-B	CMC4	Cx9C Motif-Containing protein	652594	652887	-1	Protein that localizes to the mitochondrial intermembrane space	Protein that localizes to the mitochondrial intermembrane space; localizes via the Mia40p-Erv1p system; contains twin cysteine-x(9)-cysteine motifs
S000028517	YBL039W-B	MIN6	mitochondrial MINi protein of ~6 kDa	143393	143572	1	Mitochondrial protein of unknown function	Mitochondrial protein of unknown function; mCherry fusion protein localizes to the vacuole
S000028519	YCR075W-A	EGO2	Exit from rapamycin-induced GrOwth arrest	248975	249202	1	Subunit of the EGO/GSE complex	Subunit of the EGO/GSE complex; the vacuolar/endosomal membrane associated EGO/GSE complex regulates exit from rapamycin-induced growth arrest, stimulating microautophagy and sorting of Gap1p from the endosome to the plasma membrane; identified by homology to Ashbya gossypii; EGO2 has a paralog, EGO4, that arose from the whole genome duplication 
S000028520	YDL160C-A	MHF2	Mph1-associated Histone-Fold protein	169366	169608	-1	Component of the heterotetrameric MHF histone-fold complex	Component of the heterotetrameric MHF histone-fold complex; in humans the MHF complex interacts with both DNA and Mph1p ortholog FANCM to stabilize and remodel blocked replication forks and repair damaged DNA; mhf2 srs2 double mutants are MMS hypersensitive; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-X, also known as MHF2
S000028521	YGR169C-A	LSO2	Late-annotated Small Open reading frame	836387	836665	-1	Ribosome-associated protein	Ribosome-associated protein; required for global translational elongation after recovery from stationary phase due to starvation; crosslinks to the 25S rRNA near the A site that overlaps the GTPase activation center, and to tRNAs; possible role in the iron deprivation response; localizes to the nucleus and cytoplasm, with enhanced nuclear localization under iron-replete conditions; null mutant exhibits slow growth during iron deprivation; orthologous to human CCDC124
S000028522	YJL127C-B	MCO6	Mitochondrial Class One protein of 6 kDa	181551	181709	-1	Mitochondrial protein of unknown function	Mitochondrial protein of unknown function; identified based on homology to the filamentous fungus, <i>Ashbya gossypii</i>; SWAT-GFP and seamless-GFP fusion proteins localize to the mitochondria
S000028523	YJR005C-A	LSO1	Late-annotated Small Open reading frame	448477	448758	-1	Protein with a potential role in response to iron deprivation	Protein with a potential role in response to iron deprivation; transcription increases during iron deprivation and during treatment with 2-(6-benzyl-2-pyridyl)quinazoline (BPQ) and copper; regulated by Aft1p and, to a lesser extent, by Aft2p; originally identified as a syntenic homolog of an Ashbya gossypii gene; localizes to nucleus and cytoplasm, and nuclear localization is enhanced under iron-replete conditions
S000028524	YKL068W-A	""	""	309206	309442	1	Putative protein of unknown function	Putative protein of unknown function; identified by homology to <i>Ashbya gossypii</i>
S000028525	YLR307C-A	DPA10	Delta-Psi dependent mitochondrial Assembly protein of 10 kDa	746848	747111	-1	Putative mitochondrial protein of unknown function	Putative mitochondrial protein of unknown function
S000028526	YOR020W-A	MCO10	Mitochondrial Class One protein of 10 kDa	371685	371957	1	Subunit l of the mitochondrial F1F0 ATP synthase	Subunit l of the mitochondrial F1F0 ATP synthase; F1F0 ATP synthase is a large, evolutionarily conserved enzyme complex required for ATP synthesis; more abundant in the ATP synthase monomer; involved in the calcium-induced permeability transition and calcium homeostasis; does not impact ATP synthase activity and stability; N-terminal region has sequence similarity to Atp19p; conserved in A. gossypii; detected in highly purified mitochondria in high-throughput studies
S000028527	YPL189C-A	COA2	Cytochrome Oxidase Assembly	188307	188513	-1	Cytochrome oxidase assembly factor	Cytochrome oxidase assembly factor; null mutation results in respiratory deficiency with specific loss of cytochrome oxidase activity; functions downstream of assembly factors Mss51p and Coa1p and interacts with assembly factor Shy1p
S000028528	YAL016C-B	""	""	124307	124492	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data
S000028529	YBL008W-A	""	""	209409	209648	1	Putative protein of unknown function	Putative protein of unknown function; identified by fungal homology and RT-PCR
S000028532	YBR072C-A	""	""	382860	383021	-1	Protein of unknown function	Protein of unknown function; identified by fungal homology and RT-PCR;  localizes to cytosol and peroxisomes
S000028534	YBR196C-A	""	""	614024	614173	-1	Putative protein of unknown function	Putative protein of unknown function; identified by fungal homology and RT-PCR; ORF emerged de novo in thymine-rich ancestral non-genic region, accumulated substantial changes under positive selection, and progressively increased its transmembrane propensity to give rise to a protein that integrates into the endoplasmic reticulum (ER) membrane while retaining potential for adaptive change
S000028535	YBR200W-A	""	""	622983	623147	1	Putative protein of unknown function	Putative protein of unknown function; identified by fungal homology and RT-PCR
S000028538	YDR169C-A	""	""	794574	794723	-1	Putative protein of unknown function	Putative protein of unknown function; identified by fungal homology and RT-PCR
S000028539	YDR182W-A	""	""	829151	829354	1	Putative protein of unknown function	Putative protein of unknown function; identified by fungal homology and RT-PCR
S000028541	YDR194W-A	""	""	848071	848223	1	Putative protein of unknown function	Putative protein of unknown function; identified by fungal homology and RT-PCR
S000028542	YDR246W-A	""	""	955133	955333	1	Putative protein of unknown function	Putative protein of unknown function; identified by fungal homology and RT-PCR
S000028547	YFL041W-A	""	""	48734	48925	1	Putative protein of unknown function	Putative protein of unknown function; identified by fungal homology and RT-PCR
S000028548	YGL041C-B	""	""	418704	418886	-1	Putative protein of unknown function	Putative protein of unknown function; identified by fungal homology and RT-PCR; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum
S000028550	YGR121W-A	""	""	733412	733627	1	Putative protein of unknown function	Putative protein of unknown function
S000028552	YHR086W-A	""	""	280231	280392	1	Putative protein of unknown function	Putative protein of unknown function; identified by fungal homology and RT-PCR; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum and vacuole respectively
S000028553	YHR175W-A	""	""	453558	453707	1	Putative protein of unknown function	Putative protein of unknown function; identified by fungal homology and RT-PCR
S000028556	YIL134C-A	""	""	96522	96725	-1	Putative protein of unknown function	Putative protein of unknown function; identified by fungal homology and RT-PCR
S000028557	YJR151W-A	""	""	717580	717630	1	Putative protein of unknown function	Putative protein of unknown function; identified by fungal homology and RT-PCR; predicted to have a role in transcription based on computational 'guilt by association' analysis
S000028559	YKR004C-A	""	""	447437	447679	-1	Merged open reading frame	Merged open reading frame; does not encode a discreet protein; YKR004C-A was originally annotated as an independent ORF, but was later demonstrated to be an exon of an adjacent ORF, YKR004C
S000028560	YLL006W-A	""	""	136345	136521	1	Putative protein of unknown function	Putative protein of unknown function; identified by fungal homology and RT-PCR
S000028569	YLR285C-A	""	""	708168	708338	-1	Putative protein of unknown function	Putative protein of unknown function; identified by fungal homology and RT-PCR
S000028570	YLR312C-B	""	""	760355	760642	-1	Merged open reading frame	Merged open reading frame; does not encode a discrete protein; YLR312C-B was originally annotated as an independent ORF, but as a result of a sequence change, it was merged with an adjacent ORF into a single reading frame, SPH1/YLR313C
S000028571	YLR342W-A	""	""	815810	815983	1	Putative protein of unknown function	Putative protein of unknown function
S000028572	YLR412C-A	""	""	950267	950473	-1	Putative protein of unknown function	Putative protein of unknown function
S000028573	YML054C-A	""	""	167623	167781	-1	Putative protein of unknown function	Putative protein of unknown function
S000028576	YMR230W-A	""	""	733268	733456	1	Putative protein of unknown function	Putative protein of unknown function
S000028579	YNL130C-A	DGR1	2-Deoxy-Glucose Resistant	381245	381391	-1	Protein of unknown function	Protein of unknown function; dgr1 null mutant is resistant to 2-deoxy-D-glucose
S000028580	YOL164W-A	""	""	4130	4312	1	Putative protein of unknown function	Putative protein of unknown function; identified by fungal homology and RT-PCR
S000028584	YOR316C-A	""	""	907726	907935	-1	Putative protein of unknown function	Putative protein of unknown function; identified by fungal homology and RT-PCR
S000028586	YOR376W-A	""	""	1045196	1045351	1	Putative protein of unknown function	Putative protein of unknown function; identified by fungal homology and RT-PCR
S000028588	YPL038W-A	""	""	480182	480373	1	Putative protein of unknown function	Putative protein of unknown function; identified by fungal homology and RT-PCR
S000028590	YPR108W-A	""	""	744175	744387	1	Putative protein of unknown function	Putative protein of unknown function; identified by fungal homology and RT-PCR; overlaps tRNA-Thr/YNCP0016W
S000028593	YAL067W-A	""	""	2480	2707	1	Putative protein of unknown function	Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
S000028595	YAR035C-A	""	""	192337	192417	-1	Putative protein of unknown function	Putative protein of unknown function; emerging ORF that arose de novo from non-genic locus; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; localizes to mitochondria
S000028597	YBL071C-B	""	""	89458	89556	-1	Putative protein of unknown function	Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
S000028598	YBL100W-C	""	""	28427	28546	1	Putative protein of unknown function	Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
S000028603	YBR182C-A	""	""	595361	595555	-1	Putative protein of unknown function	Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
S000028605	YBR296C-A	TYC1	Tiny Yeast Comet	800123	800242	-1	Inhibitor of the Anaphase-Promoting Complex/Cyclosome (APC/C)	Inhibitor of the Anaphase-Promoting Complex/Cyclosome (APC/C); binds to APC/C and inhibits the activity of both APC/C(Cdc20) and APC/C(Cdh1); inhibits the binding of Cdc20 and Cdh1 to APC/C; overexpression results in increased sensitivity to microtubule poison; localizes to peroxisomes; originally identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; functional homolog of human MAD2L1BP (p31comet)
S000028625	YER175W-A	""	""	540650	540814	1	Putative protein of unknown function	Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
S000028628	YFL013W-A	""	""	107799	108602	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps IES1/YFL013C; identified by gene-trapping, microarray expression analysis, and genome-wide homology searching
S000028630	YFR032C-B	MIN10	mitochondrial MINi protein of 10 kDa	223710	223973	-1	Mitochondrial protein of unknown function	Mitochondrial protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
S000028632	YGL007C-A	""	""	482941	483027	-1	Putative protein of unknown function	Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; deletion exhibits slow-growth phenotype; computationally predicted to have a role in cell budding
S000028635	YGL188C-A	""	""	148824	148964	-1	Putative protein of unknown function	Putative protein of unknown function
S000028638	YGR146C-A	""	""	785276	785437	-1	Putative protein of unknown function	Putative protein of unknown function
S000028639	YGR174W-A	""	""	846655	846741	1	Putative protein of unknown function	Putative protein of unknown function; predicted to have a role in cell budding based on computational 'guilt by association' analysis
S000028640	YGR204C-A	""	""	909061	909174	-1	Putative protein of unknown function	Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
S000028645	YHR022C-A	""	""	151217	151306	-1	Putative protein of unknown function	Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
S000028662	YJL077W-B	""	""	294047	294145	1	Putative protein of unknown function	Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
S000028667	YKL096C-B	""	""	259073	259222	-1	Putative protein of unknown function	Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
S000028683	YLR406C-A	""	""	932206	932355	-1	Putative protein of unknown function	Putative protein of unknown function; SWAT-GFP fusion protein localizes to the endoplasmic reticulum and vacuole, while mCherry fusion localizes to just the vacuole
S000028688	YML100W-A	""	""	70138	70311	1	Putative protein of unknown function	Putative protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
S000028691	YMR001C-A	""	""	271347	271577	-1	Putative protein of unknown function	Putative protein of unknown function
S000028692	YMR105W-A	""	""	478064	478258	1	Putative protein of unknown function	Putative protein of unknown function
S000028693	YMR182W-A	MIN3	mitochondrial MINi protein of 3 kDa	625811	625897	1	Mitochondrial protein of unknown function	Mitochondrial protein of unknown function
S000028694	YMR242W-A	""	""	754297	754386	1	Putative protein of unknown function	Putative protein of unknown function
S000028696	YMR272W-B	""	""	811089	811196	1	Protein of unknown function	Protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching; SWAT-GFP fusion protein localizes to the endoplasmic reticulum and vacuole, while mCherry fusion localizes to just the vacuole
S000028697	YMR315W-A	""	""	904286	904393	1	Putative protein of unknown function	Putative protein of unknown function
S000028699	YNL097C-B	PLS1	Peroxisomal Lys1 Stabilizing	440797	440919	-1	Peroxisomal matrix protein that regulates lysine biosynthesis	Peroxisomal matrix protein that regulates lysine biosynthesis; localization to peroxisomes upon lysine deprivation is dependent on targeting factor Pex5p and lysine biosynthesis enzyme Lys1p; affects abundance of Lys1p and the lysine biosynthesis pathway
S000028707	YOL019W-A	""	""	288420	288572	1	Protein of unknown function	Protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
S000028712	YOR161C-C	""	""	639121	639267	-1	Protein of unknown function	Protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
S000028721	YPL152W-A	""	""	264602	264700	1	Protein of unknown function	Protein of unknown function; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
S000028725	YPR159C-A	""	""	860314	860415	-1	Protein of unknown function	Protein of unknown function; SWAT-GFP and mCherry fusion proteins localize to the cytosol; identified by gene-trapping, microarray-based expression analysis, and genome-wide homology searching
S000028732	YAL037C-A	""	""	73426	73518	-1	Putative protein of unknown function	Putative protein of unknown function
S000028736	YBR056W-A	MNC1	MaNganese Chelating protein	351255	351455	1	Putative membrane protein	Putative membrane protein; upregulated by toxic concentrations of heavy metal ions including Mn2+, Co2+, Ni2+, Cu2+, and Zn2+; mRNA identified as translated by ribosome profiling data; localizes to the cell periphery and vacuolar membrane; CYSTM (cysteine-rich transmembrane module) family member; partially overlaps dubious ORF YBR056C-B; YBR056W-A has a paralog, YDR034W-B, that arose from the whole genome duplication
S000028739	YDR524C-B	""	""	1489598	1489798	-1	Putative protein of unknown function	Putative protein of unknown function; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum; YDR524C-B has a paralog, YCL048W-A, that arose from the whole genome duplication
S000028740	YDR524W-C	""	""	1489403	1489492	1	Putative protein of unknown function	Putative protein of unknown function; small ORF identified by SAGE; deletion strains are moderately sensitive to the radiomimetic drug bleomycin
S000028746	YER038W-A	FMP49	Found in Mitochondrial Proteome	228451	228831	1	Mitochondrial protein of unknown function	Mitochondrial protein of unknown function; almost completely overlaps ORF HVG1/YER039C
S000028747	YER046W-A	""	""	243700	244029	1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF SAP1/YER047C
S000028748	YER067C-A	""	""	292240	292563	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps ORF RGI1/YER067W
S000028749	YER068C-A	""	""	295301	295732	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps ORF ARG5,6/YER069W
S000028753	YER087C-A	""	""	331817	332368	-1	Dubious open reading frame	Dubious open reading frame; unlikely to encode a functional protein, based on available experimental and comparative sequence data; almost completely overlaps ORF AIM10/YER087W
S000028769	YGL006W-A	""	""	485423	485533	1	Putative protein of unknown function	Putative protein of unknown function; identified by SAGE
S000028804	YJL047C-A	""	""	349044	349178	-1	Putative protein of unknown function	Putative protein of unknown function
S000028806	YJL136W-A	""	""	156169	156252	1	Putative protein of unknown function	Putative protein of unknown function; identified by SAGE
S000028807	YKL158W	""	""	154996	155298	1	Merged open reading frame	Merged open reading frame; does not encode a discrete protein; YKL158W was originally annotated as an independent ORF, but was later demonstrated to be an exon of an adjacent ORF, YKL157W
S000028808	YLR264C-A	""	""	673828	673944	-1	Putative protein of unknown function	Putative protein of unknown function
S000028810	YNL067W-B	""	""	499417	499557	1	Putative protein of unknown function	Putative protein of unknown function
S000028811	YOL013W-A	""	""	301047	301238	1	Putative protein of unknown function	Putative protein of unknown function; identified by SAGE; overlaps tRNA-Pro/YNCO0011W
S000028812	YOL038C-A	""	""	254926	255021	-1	Putative protein of unknown function	Putative protein of unknown function; identified by SAGE analysis
S000028816	YBR196C-B	""	""	614526	614630	-1	Putative protein of unknown function	Putative protein of unknown function; identified by expression profiling and mass spectrometry
S000028817	YBR221W-A	""	""	666538	666642	1	Putative protein of unknown function	Putative protein of unknown function; identified by expression profiling and mass spectrometry
S000028819	YDR003W-A	""	""	454782	454904	1	Putative protein of unknown function	Putative protein of unknown function; identified by expression profiling and mass spectrometry
S000028827	YGR035W-A	""	""	557565	557786	1	Putative protein of unknown function	Putative protein of unknown function
S000028828	YHL015W-A	""	""	74699	74782	1	Putative protein of unknown function	Putative protein of unknown function
S000028830	YHR007C-A	""	""	122550	122765	-1	Putative protein of unknown function	Putative protein of unknown function; identified by expression profiling and mass spectrometry; SWAT-GFP fusion protein localizes to the nucleus
S000028832	YHR050W-A	""	""	209475	209645	1	Protein of unknown function	Protein of unknown function; identified by expression profiling and mass spectrometry
S000028835	YIL002W-A	CMI7	Cytosolic MIni protein of ~7 kDa	350301	350510	1	Putative mitochondrial protein of unknown function	Putative mitochondrial protein of unknown function; identified by expression profiling and mass spectrometry
S000028836	YIL046W-A	""	""	268309	268473	1	Putative protein of unknown function	Putative protein of unknown function; identified by expression profiling and mass spectrometry
S000028837	YIR018C-A	""	""	385564	385701	-1	Putative protein of unknown function	Putative protein of unknown function; identified by expression profiling and mass spectrometry
S000028838	YIR021W-A	""	""	398514	398726	1	Putative protein of unknown function	Putative protein of unknown function; identified by expression profiling and mass spectrometry
S000028842	YKR099C-A	""	""	638531	638722	-1	Merged open reading frame	Merged open reading frame; does not encode a protein; YKR099C-A was originally annotated as an independent ORF, but as a result of a sequence change, it was merged with an adjacent ORF into a single reading frame, designated YKR100C
S000028845	YLR361C-A	""	""	849383	849679	-1	Putative protein of unknown function	Putative protein of unknown function
S000028848	YMR175W-A	""	""	611314	611508	1	Putative protein of unknown function	Putative protein of unknown function
S000028849	YMR247W-A	""	""	769283	769426	1	Putative protein of unknown function	Putative protein of unknown function; SWAT-GFP fusion protein localizes to the endoplasmic reticulum and vacuole, while mCherry fusion localizes to just the vacuole
S000028850	YNL042W-B	""	""	547113	547370	1	Putative protein of unknown function	Putative protein of unknown function
S000028851	YNL146C-A	""	""	351386	351580	-1	Putative protein of unknown function	Putative protein of unknown function
S000028852	YNL277W-A	""	""	116679	116867	1	Putative protein of unknown function	Putative protein of unknown function
S000028854	YOL097W-A	""	""	136220	136405	1	Putative protein of unknown function	Putative protein of unknown function; identified by expression profiling and mass spectrometry
S000028856	YOR034C-A	""	""	397426	397668	-1	Putative protein of unknown function	Putative protein of unknown function; identified by expression profiling and mass spectrometry
S000028858	YOR293C-A	""	""	867998	868147	-1	Putative protein of unknown function	Putative protein of unknown function; identified by expression profiling and mass spectrometry
S000028859	YPL119C-A	""	""	324024	324287	-1	Putative protein of unknown function	Putative protein of unknown function; identified by expression profiling and mass spectrometry
S000029705	YCL012C	""	""	101317	101788	-1	Protein of unknown function	Protein of unknown function; SWAT-GFP and mCherry fusion proteins localize to the endoplasmic reticulum and vacuole respectively; orthologs are present in S. bayanus, S. paradoxus and Ashbya gossypii; YCL012C is not an essential gene
S000029722	YBR230W-A	COQ21	COQ interacting protein	680362	680562	1	Mitochondrial protein of unknown function	Mitochondrial protein of unknown function; YBR230W-A has a paralog, COQ8, that arose from the whole genome duplication
S000029724	YCR095W-A	""	""	289639	289797	1	Putative protein of unknown function	Putative protein of unknown function
S000029726	YGR161W-C	""	""	810227	810505	1	Putative protein of unknown function	Putative protein of unknown function; identified by sequence comparison with hemiascomycetous yeast species
S000082441	YML033W	""	""	211715	212155	1	Merged open reading frame	Merged open reading frame; does not encode a discrete protein; YML033W was originally annotated as an independent ORF, but was later demonstrated to be an exon of an adjacent ORF, YML034W
S000115154	YLR391W	""	""	903929	904387	1	Merged open reading frame	Merged open reading frame; does not encode a discrete protein; YLR391W was originally annotated as an independent ORF, but was completely contained in larger ORF YLR391W-A in the same frame, and was merged into it on 7-30-1997
S000117700	YER108C	""	""	375211	376995	-1	Merged ORF	Merged ORF; YER108C has been merged into YER109C because it has been demonstrated that they correspond to a mutated FLO8 gene with an A to G transition at position 431 in strain S288C, changing amino acid 144 from a Trp to a stop
S000120182	YAR043C	""	""	192928	193248	-1	Deleted ORF	Deleted ORF; does not encode a protein; included in the original annotation of Chromosome I but later withdrawn