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- %% CALLED By p4loop8c.m %%
- function [p4L2, TmpexpScanIntens3,TmpFexpScanSpots3,TmpFexpScanBMtp3,TmpanlZoneRefs3,Tmpsbdg3]= ...
- par4GblFnc8c(parMat,tptLength,~,selScanNumLst,~,Fflg,PTmapPos,optCirMask,diaExt,doCircle,cirPixA,cirMask,~,...
- TmpexpScanIntens,TmpFexpScanSpots,TmpFexpScanBMtp,TmpanlZoneRefs,scCount,tifFileLstP4,~,ImParMat, ...
- numRows,numCols,scLst,easyResultsDir,projectScansDir,~, Tmpsbdg)
- global printResultsDir
- selScan=selScanNumLst(scCount);
- tptLength0=length(tifFileLstP4{scCount});
- % tptLength=numFiles;
- tifFileLst={tifFileLstP4(scCount)};
-
- % Extract the Imaging time stamps from selected tif files
- % clear('e','f'); %can't use clear inside a parfor loop. Preallocation
- % can be larger than useable .bmp files! Therefore must be small and
- % increased during for loop to maintain cell size integrity between e & f
- e=cell(1,2); f=cell(1,2); %(tptLength,2); f=cell(tptLength,2);
- nndx=0;
- for tPt=1:tptLength0 %size(tifFileLst,1) %length(tifFileLst)
- tifFileLstP4{scCount}(tPt)
- scLst;
- scLst(scCount)
- char(scLst(scCount))
- char(fullfile(projectScansDir,char(scLst(scCount))))
- projectScansDir;
- swCatch=0;
- nndx=nndx+1;
- tifFile=char(fullfile(projectScansDir,char(scLst(scCount)), tifFileLstP4{scCount}(tPt)));
- try
- info=imfinfo(tifFile); % D:\jwrDevel\DevelCurveFittingJWR\ImageScans\Scan2\020hr002.tif
- catch ME
- % Note: During parallel operation Matlab will not open file (fid)
- % etc. Therefore error message text will not be written.The only way
- % to get it out would be to pass variable through called function to
- % the ..Main8c script outside the parfor loop and print to file from
- % there. Consequently for now it only prints error to file when one
- % edits p4loop8c.m from 'parfor' to ordinary 'for' loop
- fFail=((fullfile(printResultsDir,'ImageFileReadFailure.txt')));
- fid=fopen(fFail,'a'); %create,open and append
- % fprintf(fid,'%s \n',char(tifFile))
- fclose(fid);
- nndx=nndx-1;
- swCatch=1;
- rep=getReport(ME, 'basic');
- rep=strcat('Read info failure for-',tifFile,' -', rep);
- % fprintf(fid,'%s \n',rep) %See Note:
- end
- tptLength=nndx;
- scTmNumeric=1; %initialize for parfor
- if swCatch==0
- scTmNumeric(nndx)=datenum(info.FileModDate);
- e(nndx,:)={tifFile, scTmNumeric(nndx)};
- % newtifFileLst(nndx)=tifFileLst(tPt);
- end
- end
- % Clear tifFileLst;
- tifFileLst=cell(nndx,1);
- f={sortrows(e,2)};
- tifFileLst=f{1,1}(:,1);
- areaOfIntensAboveBG=zeros(24,16,length(tifFileLst));
- % Calculate Time series for each Plate of Selected Scan
- lastPlateOnScan=1; %Ncode
- tSeriesv=[]; t0Seriesv=[];
- if tptLength>0 % added to jump over and fill data for invalid Sscan(plate runs
- scTmNumv=cell2mat(f{1,1}(:,2)); % (:,2))120613 fix for variant length data
- prtTmNumv=min(scTmNumv)-.000001;
- tSeriesv=((scTmNumv-prtTmNumv)*24);
- t0Seriesv=((scTmNumv-scTmNumv(1))*24);
- end
- % Added to jump over and fill data for invalid Sscan(plate runs
- if tptLength>=3
- % Create blank scan
- ImHeigth=2075;
- ImWidth=1400;
- Empsc=zeros(ImHeigth,ImWidth); %Ncode
- % Start scan loop
- lastPlateOnScan=1; %Ncode
- disp('Before call to NIscanIntens.....')
- % Execute Image conversion into Intensity Data
- [Tmpsbdg2, scanIntens, ~, ~, ~, TmpexpScanIntens2, TmpFexpScanSpots2, TmpFexpScanBMtp2, TmpanlZoneRefs2,areaOfIntensAboveBG]= ...
- NIscanIntensBGpar4GblFnc(Fflg,tifFileLst, ImParMat, PTmapPos,optCirMask,diaExt,doCircle,cirPixA,numRows,numCols,ImHeigth,ImWidth,cirMask, ...
- tptLength,selScan,Empsc,TmpexpScanIntens,TmpFexpScanSpots,TmpFexpScanBMtp,TmpanlZoneRefs,easyResultsDir, Tmpsbdg);
- TmpexpScanIntens3=TmpexpScanIntens2;
- TmpFexpScanSpots3=TmpFexpScanSpots2;
- TmpFexpScanBMtp3=TmpFexpScanBMtp2;
- TmpanlZoneRefs3=TmpanlZoneRefs2;
- Tmpsbdg3=Tmpsbdg2;
- % clear plate
- CFscanIntens=zeros(384,1); % zeros(16,24);
- plate=[];
- plate=zeros(24,16,tptLength);
- plate(:,:,:,1)=scanIntens(1:1:24,16:-1:1,:); % TmpexpScanIntens2(1:1:24,16:-1:1,:);%Ncode Dev3Vertical Similar to below
- % NIcheck: Check for bad scans at time points (swapped plates etc.)
- % Set up cell arrays for storing each plate in each Scan ..(scan,plate)
- SWprint=0;
- % Construct Legacy ...Intens.txt file
- if SWprint==1
- filename=fullfile(easyResultsDir,'PrintResults',(strcat('Plate',num2str(selScan),'_Intens.txt')));
- fid=fopen(filename,'w');
- end
- if length(size(plate))==2 % only two dims if only one image
- numOfImages=1;
- else
- numOfImages=size(plate,3);
- end
- CFscanIntens=zeros(384,numOfImages);
- locIndx=0; pl=1;
- for n=1:numCols % Ncode changed to 16 for Vert
- for m=1:numRows % Ncode change to 24 for Vert
- locIndx=locIndx+1;
- for k=1:numOfImages
- if SWprint==1
- if k==1 && numOfImages==1, fprintf(fid,'%.2f\n',plate(m,n,k,pl));end
- if k==1 && numOfImages~=1, fprintf(fid,'%.2f',plate(m,n,k,pl));end
- if k>1 && k<numOfImages, fprintf(fid,'\t%.2f',plate(m,n,k,pl));end %
- if k~=1 && k==numOfImages,fprintf(fid,'\t%.2f\n',plate(m,n,k,pl));end
- end
- CFscanIntens(locIndx,k)=plate(m,n,k,pl);
- end
- end
- end
- if SWprint==1
- fclose(fid);
- end
- % printGrwAreas
- % Area as the max number of pixels with intensities greater than the Background
- % grArea(row,col,tPt,Plate) Note that images r and c are 90 degrees shifted
- % and then mirror imaged. Thus data must be inverted and rotated to get
- % for the rows a-p and col 1-24 (and the EMS expected 1-384) orientation
- % clear grArea
- grArea=ones(24,16,tptLength);
- grArea(:,:,:,1)=areaOfIntensAboveBG(1:1:24,16:-1:1,:); %for Ncode dev3vert
- % for p=1:lastPlateOnScan
- if SWprint==1
- if SWgrowthArea==1
- filename=fullfile(printResultsDir,(strcat('Plate',num2str(selScan),'_Areas.txt')));
- else
- filename=fullfile(printResultsDir,(strcat('ALTgrowthA_Scan',num2str(selScan),'_Plate',num2str(1),'_Areas.txt')));
- filename=fullfile(printResultsDir,(strcat('ALTgrowthA_Plate',num2str(selScan),'_Plate','_Areas.txt')));
- end
- fid=fopen(filename,'w');
- end
- locIndx=0;
- Ag=zeros(1,384);
- for m=1:16 % Ncode change from 16 %Vert back to 16
- for n=1:24 % Ncode change from 24 %Vert back to 24
- growthArea=max(grArea(n,m,:,1));
- if SWprint==1
- fprintf(fid,'%3.f\n',growthArea);
- end
- locIndx=locIndx+1;
- Ag(locIndx)=growthArea;
- end
- end
- if SWprint==1
- fclose(fid);
- % fclose('all'); % TODO do we need this?
- end
- % AutoImCF curveFit kickoff setup
- autoImCFlag=1;
- if autoImCFlag==1, SWgrowthArea=1;end
- times=tSeriesv;
- scanAreas=Ag; % growthArea;
- offsets=0;
- suffix=strcat('Scan', num2str(selScan),'_Plate', num2str(1)); % char(QspLst(n));
- AUCfinalTime=96;
- arrayFormat=384;
-
- % Version compatability fixes
- %{
- v11a='7.12.0.635 (R2011a)';
- v14a='8.3.0.532 (R2014a)';
- %v14b='8.4.0.150421 (R2014b)';
- v18a='9.4.0.813654 (R2018a)';
- v18b='9.5.0.944444 (R2018b)';
- % Fourth order derivative of logistic equation
- if isequal(v18a,version)||isequal(v18b,version)
- syms t K r l;
- fd4=diff(K / (1 + exp(-r* (t - l ))),t,4);
- else
- fd4=diff(sym('K / (1 + exp(-r* (t - l )))'),4);
- end
- sols=solve(fd4);
- if isequal(v11a,version) || isequal(v14a,version)
- else
- tmpswap=sols(1);
- sols(1)=sols(3);
- sols(3)=tmpswap;
- end
- %}
- if verLessThan('matlab','8.3') % original work 23_0227 updated 23_0525 (8.4 changed to 8.3)
- fd4=diff(sym('K / (1 + exp(-r* (t - l )))'),4);
- sols=solve(fd4);
- else % accomodate new matlab changes after 2014a fix 2nd update 23_0227
- syms t K r l;
- fd4=diff(K / (1 + exp(-r* (t - l ))),t,4);
- sols=solve(fd4);
- tmpswap=sols(1);
- sols(1)=sols(3);
- sols(3)=tmpswap;
- end
- % CFscanIntens
- % Call CurveFit routine
- % NCfitImCF(times, CFscanIntens, offsets, suffix, AUCfinalTime, arrayFormat, scanAreas, printResultsDir, autoImCFlag, selScan,sols) %,scan)%, scPltList) outputDirectory;
- % [scanTmp2]=NCfitImCFparforFailGbl(times, CFscanIntens, offsets, suffix, AUCfinalTime, arrayFormat, scanAreas, printResultsDir, autoImCFlag, selScan, sols, scanTmp); %,scan)%, scPltList) outputDirectory;
- [par4scanselIntensStd,par4scanselTimesStd,par4scanTimesELr,par4scanIntensELr,par4scanCFparameters,par4scanCFdate,outC2,outCstd2]= ...
- NCfitImCFparforFailGbl2(parMat,times, CFscanIntens, offsets, suffix, AUCfinalTime, arrayFormat, scanAreas, printResultsDir, autoImCFlag, selScan, sols); %,scan)%, scPltList) outputDirectory;
- else %fill with default values when an invalid plate scan occurs
- CFscanIntens=zeros(16,24);
- Ag=zeros(1,384);
- % Preallocation for parfor loop
- times=tSeriesv;
- st(1,1:size(times,2))=1111;
- resMat(1,1:27)=0;
- resMatStd=resMat;
- outC2=zeros(384,27);
- outCstd2=zeros(384,27);
- for m=1:384
- pa{m}=st;
- par4scanCFparameters{m}=parMat;
- par4scanCFdate{m}=datestr((now),31);
- end
- par4scanselTimesStd=pa;
- par4scanselIntensStd=pa;
- par4scanTimesELr=pa;
- par4scanIntensELr=pa;
- par4resMat=zeros(384,27);
- par4resMatStd=zeros(384,27);
- %{
- TmpexpScanIntens00=cell(1); %cell(1,scanMax);
- TmpFexpScanSpots00=cell(1); %cell(1,scanMax);
- TmpFexpScanBMtp00=cell(1); %cell(1,scanMax);
- TmpanlZoneRefs00=cell(1); %cell(1,scanMax);
- Tmpsbdg00=cell(1);
- %}
- TmpexpScanIntens3=cell(1); %TmpexpScanIntens2;
- TmpFexpScanSpots3=cell(1); %TmpFexpScanSpots2;
- TmpFexpScanBMtp3=cell(1); %TmpFexpScanBMtp2;
- TmpanlZoneRefs3=cell(1); %TmpanlZoneRefs2;
- Tmpsbdg3=cell(1); %Tmpsbdg2;
- end %if tptLenth>=3 line19 20_0928
- tSeriesv; %debuggin parfor
- p4L1{1}=tSeriesv;
- p4L1{2}=t0Seriesv;
- p4L1{3}=datestr(prtTmNumv,31);
- p4L1{4}=CFscanIntens;
- locIndx=0;
- for n=1:numCols % Ncode changed to 16 for Vert
- for m=1:numRows % Ncode change to 24 for Vert
- locIndx=locIndx+1;
- rc=[n,m];
- p4rcTmp(locIndx)={rc};
- p4pIndxTmp(locIndx)=locIndx;
- end
- end
- p4L1{5}=p4rcTmp;
- p4L1{6}=p4pIndxTmp;
- p4L1{7}=Ag;
- p4L1{8}=par4scanselIntensStd;
- p4L1{9}=par4scanselTimesStd;
- p4L1{10}=par4scanTimesELr;
- p4L1{11}=par4scanIntensELr;
- p4L1{12}=par4scanCFparameters;
- p4L1{13}=par4scanCFdate;
- p4L1{14}=outC2;
- p4L1{15}=outCstd2;
- p4L1{16}=selScan;
- p4L1{17}=cirPixA;
- p4L1{18}=datestr((now),31); % TODO this seems bad
- p4L2=p4L1;
- end
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