123456789101112131415161718192021222324252627282930313233343536373839404142434445464748495051525354555657585960616263646566676869707172737475767778798081828384858687888990919293949596979899100101102103104105106107108109110111112113114115116117118119120121122123124125126127128129130131132133134135136137138139140141142143144145146147148149150151152153154155156157158159160161162163164165166167168169170171172173174175176177178179180181182183184185186187188189190191192193194195196197198199200201202203204205206207208209210211212213214215216217218219220221222223224225226227228229230231232233234235236237238239240241242243244245246247248249250251252253254255256257258259260261262263264265266267268269270271272273274275276277278279280281282283284285286287288289290291292293294295296297298299300301302303304305306307308309310311312313314315316317318319320321322323324325326327328329330331332333334335336337338339340341342343344345346347348349350351352353354355356357358359360361362363364365366367368369370371372373374375376377378379380381382383384385386387388389390391392393394395396397398399400401402403404405406407408409410411412413414415416417418419420421422423424425426427428429430431432433434435436437438439440441442443444445446447448449450451452453454455456457458459460461462463464465466467468469470471472473474475476477478479480481482483484485486487488489490491492493494495496497498499500501502503504505506507508509510511512513514515516517518519520521522523524525526527528529530531532533534535536537538539540541542543544545546547548549550551552553554555556557558559560561562563564565566567568569570571572573574575576577578579580581582583584585586587588589590591592593594595596597598599600601602603604605606607608609610611612613614615616617618619620621622623624625626627628629630631632633634635636637638639640641642643644645646 |
- % EZmultiGeneRFsLst Called by EZmultiExLoad
- % Called by EZmultiExLoad about line173 Which is called by EZexpSel
- % User Select Reference Gene and Specifics Added
- % Compile GeneList and OrfList
- % Sort and add the medians of Ref Plates if RF1,RF2 exist
- clear aa bb c uu vv drf drfPindx drfr drfc
- clear RF1scanN RF2scanN
- for mx=1:length(Exp(expN).Dexp(n).MP)
- try
- char((Exp(expN).Dexp(n).MP(mx).genename{1}(384)))
- MPnum=mx;
- catch
- break
- end
- end
- DMnum=length(Exp(expN).Dexp(n).DM.drug);
- tPtsSize=size(Exp(expN).Dexp(n).FexpScanBMtp{1,1},(3)); %size(FexpScanBMtp{1,1},3);
- lstindx=0;
- lstindxOrf=0;
- spN=0;
- Exp(expN).Dexp(n).RFrnames %<--???
- rfcnt=0;
- dRF1indx=0;
- RF1mp=[]; RF2mp=[];
- rfSp=cell2mat(rfSpec); % user entry of Specifics for Ref selection
- for mp=1:MPnum
- % RF1fullPlate=0;
- for ind384=1:384
- spN=spN+1;
- try
- r=ceil(ind384/24);
- if rem(ind384,24)==0, c=24; else c=rem(ind384,24); end
- % Insert test for numeric in genename and orf if isnumeric
- % Correct common EXCEL problem of converting OCT1 into a date numeric
- if cell2mat(Exp(expN).Dexp(n).MP(mp).genename{1}(ind384))==38991,
- Exp(expN).Dexp(n).MP(mp).genename{1}(ind384)={'OCT1_'};
- elseif isnumeric(cell2mat(Exp(expN).Dexp(n).MP(mp).genename{1}(ind384)))
- Exp(expN).Dexp(n).MP(mp).genename{1}(ind384)={' '};
- end
- %****Check for full(384spot) Reference Plates (RF1 or/and RF2)**************
- RF1fullPlate=0;
- try RF1fullPlate=sum(ismember((Exp(expN).Dexp(n).MP(mp).genename{1}),rfGene))==384; catch, mp, msg=strcat(num2str(mp),' genename ERROR'), end
- RF2fullPlate=0;
- try RF2fullPlate=sum(ismember((Exp(expN).Dexp(n).MP(mp).genename{1}),'RF2'))==384; catch, mp, msg=strcat(num2str(mp),' genename ERROR'), end
- % Capture non-reference spot location and ORF data
- if isempty(regexpi(char((Exp(expN).Dexp(n).MP(mp).orf{1}(ind384))),'blank'))... %'\<blank\>'
- && ~isnumeric(Exp(expN).Dexp(n).MP(mp).orf{1}(ind384))...
- && ~strcmpi((Exp(expN).Dexp(n).MP(mp).orf{1}(ind384)),' ')...
- && ~strcmpi((Exp(expN).Dexp(n).MP(mp).orf{1}(ind384)),' ')...
- && ~strcmpi((Exp(expN).Dexp(n).MP(mp).orf{1}(ind384)),'')...
- && ~isempty((Exp(expN).Dexp(n).MP(mp).orf{1}(ind384)))...
- && ((~strcmpi((Exp(expN).Dexp(n).MP(mp).genename{1}(ind384)),rfGene)|| ~strcmpi((Exp(expN).Dexp(n).MP(mp).specifics{1}(ind384)),rfSp))...
- && (~strcmpi((Exp(expN).Dexp(n).MP(mp).genename{1}(ind384)),rfGene)|| ~strcmpi(rfSp,'None')))...
- && ((~strcmpi((Exp(expN).Dexp(n).MP(mp).orf{1}(ind384)),rfGene)|| ~strcmpi((Exp(expN).Dexp(n).MP(mp).specifics{1}(ind384)),rfSp))...
- && (~strcmpi((Exp(expN).Dexp(n).MP(mp).orf{1}(ind384)),rfGene)|| ~strcmpi(rfSp,'None')))...
- && (~strcmpi((Exp(expN).Dexp(n).MP(mp).orf{1}(ind384)),'RF2')||~strcmpi((Exp(expN).Dexp(n).MP(mp).genename{1}(ind384)),'RF2'))...
- && iscellstr((Exp(expN).Dexp(n).MP(mp).orf{1}(ind384)))
- lstindxOrf=lstindxOrf+1;
- Exp(expN).Dexp(n).MP(mp).orf{1}(ind384)=strrep((Exp(expN).Dexp(n).MP(mp).orf{1}(ind384)),':',' ');
- bb(lstindxOrf,1)=(Exp(expN).Dexp(n).MP(mp).orf{1}(ind384));
- bb(lstindxOrf,2)={strcat(':',num2str(mp),':',num2str(r),':',num2str(c))};
- bb(lstindxOrf,3)={mp};
- bb(lstindxOrf,4)={r};
- bb(lstindxOrf,5)={c};
- mutorfs(lstindxOrf)=spN; %16_0318 added for Interaction EZinteract
- % Exp(expN).Dexp(n).mutSpotIndx(lstindxOrf)=spN; %16_0318 added for Interaction EZinteract
- end
- % Capture non-reference spot location and GENENAME data
- if isempty(regexpi(char((Exp(expN).Dexp(n).MP(mp).genename{1}(ind384))),'blank'))... %'\<blank\>'
- && ~isnumeric(Exp(expN).Dexp(n).MP(mp).genename{1}(ind384))...
- && ~strcmpi((Exp(expN).Dexp(n).MP(mp).genename{1}(ind384)),' ')...
- && ~strcmpi((Exp(expN).Dexp(n).MP(mp).genename{1}(ind384)),' ')...
- && ~strcmpi((Exp(expN).Dexp(n).MP(mp).genename{1}(ind384)),'')...
- && ~isempty((Exp(expN).Dexp(n).MP(mp).genename{1}(ind384)))...
- && (~strcmpi((Exp(expN).Dexp(n).MP(mp).genename{1}(ind384)),rfGene)...
- || ~strcmpi((Exp(expN).Dexp(n).MP(mp).specifics{1}(ind384)),rfSp))... % AddSpecifics rfSp && ~RF1fullPlate)...
- && (~strcmpi((Exp(expN).Dexp(n).MP(mp).genename{1}(ind384)),rfGene) || ~strcmpi(rfSp,'None'))...
- && (~strcmpi((Exp(expN).Dexp(n).MP(mp).genename{1}(ind384)),'RF2'))... % && ~RF2fullPlate)...
- && iscellstr((Exp(expN).Dexp(n).MP(mp).genename{1}(ind384)))
- lstindx=lstindx+1;
- Exp(expN).Dexp(n).MP(mp).genename{1}(ind384)=strrep((Exp(expN).Dexp(n).MP(mp).genename{1}(ind384)),':',' ');
- aa(lstindx,1)=(Exp(expN).Dexp(n).MP(mp).genename{1}(ind384));
- aa(lstindx,2)={strcat(':',num2str(mp),':',num2str(r),':',num2str(c))};
- aa(lstindx,3)={mp};
- aa(lstindx,4)={r};
- aa(lstindx,5)={c};
- mutgenes(lstindx)=spN;
- elseif (RF1fullPlate==1 ... % sum(ismember((Exp(expN).Dexp(n).MP(mp).genename{1}),rfGene))==384 || ...
- || (((length(unique(Exp(expN).Dexp(n).MP(mp).genename{1}(1:384))))==1 && mp==1 ) ... % (isequal((Exp(expN).Dexp(n).MP(mp).genename{1}(1:384)), (Exp(expN).Dexp(n).MP(mp).genename{1}(384:-1:1)))&& mp==1 )) && ...
- && ~exist('RF1scanN','var')))
- RF1mp=mp
- RF1scanN=(mp*DMnum)-(DMnum-1)
- rfcnt=rfcnt+1;
- RFs(spN:spN+383)=spN:spN+383;
- elseif (RF2fullPlate==1|| ... % sum(ismember((Exp(expN).Dexp(n).MP(mp).genename{1}(ind384)),'RF2'))==384 ||...
- (((length(unique(Exp(expN).Dexp(n).MP(mp).genename{1}(1:384))))==1 && mp==MPnum ) && ...
- ~exist('RF2scanN','var')))
- RF2mp=mp
- rfcnt=rfcnt+1;
- RFs(spN:spN+383)=spN:spN+383;
- RF2scanN=(mp*DMnum)-(DMnum-1)
- % DISPersed REFerence capture and find Medians
- elseif ( ((strcmpi((Exp(expN).Dexp(n).MP(mp).genename{1}(ind384)),rfGene) ...
- && strcmpi((Exp(expN).Dexp(n).MP(mp).specifics{1}(ind384)),rfSp))) ...
- || ((strcmpi((Exp(expN).Dexp(n).MP(mp).genename{1}(ind384)),rfGene) ...
- && strcmpi(rfSp,'None'))) ) &&(RF1fullPlate~=1)
- dRF1indx=dRF1indx+1;
- Exp(expN).Dexp(n).MP(mp).genename{1}(ind384)=strrep((Exp(expN).Dexp(n).MP(mp).genename{1}(ind384)),':',' ');
- drf(dRF1indx,1)=(Exp(expN).Dexp(n).MP(mp).genename{1}(ind384));
- drf(dRF1indx,2)={strcat(':',num2str(mp),':',num2str(r),':',num2str(c))};
- drf(dRF1indx,3)={mp}; drfMP(dRF1indx,mp)=mp;
- drf(dRF1indx,4)={r}; drfr(dRF1indx,mp)=r;
- drf(dRF1indx,5)={c}; drfc(dRF1indx,mp)=c;
- drf(dRF1indx,6)={ind384}; drfPindx(dRF1indx,mp)=ind384;
- % drf(dRF1indx,7)=(Exp(expN).Dexp(n).MP(mp).specifics{1}(ind384));
- end % if isempty(regexpi(char((Exp(expN).Dexp(n).MP(mp).genename{1}(ind384))),'blank'))...
- catch
- mp % Disable in future to prevent a bomb out OR put into a nested TryCatch
- ind384 % Disable in future to prevent a bomb out OR put into a nested TryCatch
- msgBadGeneName=strcat('check genename at mp=',num2str(mp),' indx=',num2str(ind384))
- end
- end
- mp
- end
- % Cover case if mutorfs RFs are labeled RF1 or RF2
- try
- Exp(expN).Dexp(n).mutSpotIndx.wRFs=union(RFs, mutorfs);
- catch
- msg='No RFs Found in Exp! wRFs'
- Exp(expN).Dexp(n).mutSpotIndx.wRFs=mutorfs;
- end
- try
- Exp(expN).Dexp(n).mutSpotIndx.woRFs=setdiff(mutorfs,RFs);
- catch
- msg='No RFs Found in Exp! woRFs'
- Exp(expN).Dexp(n).mutSpotIndx.woRFs=mutorfs;
- end
-
- Exp(expN).Dexp(n).RFmd1indx=[];
- Exp(expN).Dexp(n).RFmd1pltN=[];
- Exp(expN).Dexp(n).RFmd1LB={[]};
- Exp(expN).Dexp(n).RFmd1indx=[];
- Exp(expN).Dexp(n).RFmd1pltN={};
- Exp(expN).Dexp(n).RFmd1val=[];
- Exp(expN).Dexp(n).mean1=[];
- Exp(expN).Dexp(n).std1=[];
- Exp(expN).Dexp(n).min1=[];
- Exp(expN).Dexp(n).max1=[];
- Exp(expN).Dexp(n).RFcmpK=[]; %For Ref Composite 17_1009
- Exp(expN).Dexp(n).RFcmpr=[]; %For Ref Composite 17_1009
- Exp(expN).Dexp(n).RFcmpL=[]; %For Ref Composite 17_1009
- Exp(expN).Dexp(n).RFmd2LB={[]};
- Exp(expN).Dexp(n).RFmd2indx=[];
- Exp(expN).Dexp(n).RFmd2pltN=[];
- Exp(expN).Dexp(n).RFmd2val=[];
- Exp(expN).Dexp(n).mean2=[];
- Exp(expN).Dexp(n).std2=[];
- Exp(expN).Dexp(n).min2=[];
- Exp(expN).Dexp(n).max2=[];
- Exp(expN).Dexp(n).RFmean=[];
- Exp(expN).Dexp(n).RFstd=[];
- Exp(expN).Dexp(n).RFmin=[];
- Exp(expN).Dexp(n).RFmax=[];
- Exp(expN).Dexp(n).RFmdPLB={[]};
- Exp(expN).Dexp(n).RFmdPindx=[];
- Exp(expN).Dexp(n).RFmdPpltN=[];
- Exp(expN).Dexp(n).RFmdPscanN=[];
- Exp(expN).Dexp(n).RFmedianP=[];
- Exp(expN).Dexp(n).stdP=[];
- Exp(expN).Dexp(n).meanP=[];
- Exp(expN).Dexp(n).minP=[];
- Exp(expN).Dexp(n).maxP=[];
- Exp(expN).Dexp(n).RFmdGLB={[]};
- Exp(expN).Dexp(n).RFmdGindx=[];
- Exp(expN).Dexp(n).RFmdGpltN=[];
- Exp(expN).Dexp(n).RFmdGscanN=[];
- Exp(expN).Dexp(n).RFmedianG=[];
- Exp(expN).Dexp(n).RFminG=[];
- Exp(expN).Dexp(n).RFmaxG=[];
- Exp(expN).Dexp(n).RFstdG=[];
- Exp(expN).Dexp(n).RFmeanG=[];
- Exp(expN).Dexp(n).RFcmpGK=[]; %For Ref Composite 17_1009
- Exp(expN).Dexp(n).RFcmpGr=[]; %For Ref Composite 17_1009
- Exp(expN).Dexp(n).RFcmpGL=[]; %For Ref Composite 17_1009
- if exist('RF1scanN','var')
- for d=0:(DMnum-1)
- medianIndx=[];
- nonZeroIndx=[];
- medValList=[];
- rf1scN=RF1scanN+d;
- try %if RF1scanN doesn't exist this addition to list will be skipped
- nonZeroIndx=find(Exp(expN).Dexp(n).scan(rf1scN).plate(1).CFout(1:384,5));
-
- if isempty(nonZeroIndx)
- nonZeroCnt=0
- medianIndx=nonZeroIndx;
- else
- nonZeroCnt=length(nonZeroIndx);
- end
- if ~isempty(nonZeroIndx) && mod(nonZeroCnt,2)==0, medianIndx=nonZeroIndx(1:nonZeroCnt-1); end
- if ~isempty(nonZeroIndx) && mod(nonZeroCnt,2)~=0, medianIndx=nonZeroIndx; end
- clear medValLst
- clear kValLst
- clear rValLst
- if ~isempty(nonZeroIndx) && length(medianIndx) > (.15*384) % To calc. a median, more than 15% of spots must be nonZero
- medValLst=Exp(expN).Dexp(n).scan(rf1scN).plate(1).CFout(medianIndx,5);
- medValList1{d+1}=medValLst;
- min1=min(medValLst);
- max1=max(medValLst);
- std1=std(medValLst);
- mean1=mean(medValLst);
- kValLst=Exp(expN).Dexp(n).scan(rf1scN).plate(1).CFout(medianIndx,3); % For Ref Composite 17_1009
- rValLst=Exp(expN).Dexp(n).scan(rf1scN).plate(1).CFout(medianIndx,4); % For Ref Composite 17_1009
- mval1=median(medValLst); %mval1=median(Exp(expN).Dexp(n).scan(RF1mps).plate(1).CFout(1:384,5)); %CFout(1:383,5));
- RFmd1pos=find(mval1==(Exp(expN).Dexp(n).scan(rf1scN).plate(1).CFout(1:384,5)));
- RFmd1loc(1)=ceil(RFmd1pos/24); %calc r value
- RFmd1loc(2)=RFmd1pos - ((RFmd1loc(1)-1)*24); %calc c value
- lstindx=lstindx+1;
- lstindxOrf=lstindxOrf+1;
- aa(lstindx,1)={strcat('RF1md(',num2str(d+1),')')}; %{'RF1md'};
- aa(lstindx,2)={strcat(':',num2str(RF1mp),':',num2str(RFmd1loc(1)),':',num2str(RFmd1loc(2)))}; %,'std_',num2str(std1))};
- bb(lstindxOrf,1)={strcat('RF1md(',num2str(d+1),')')}; %remove string2cell brackets {...} %{'RF1md'};
- bb(lstindxOrf,2)={strcat(':',num2str(RF1mp),':',num2str(RFmd1loc(1)),':',num2str(RFmd1loc(2)))}; %,'std_',num2str(std1))};
- % Composite Reference Amalgum Plot
- lstindx=lstindx+1;
- lstindxOrf=lstindxOrf+1;
- aa(lstindx,1)={strcat('RF1cmp(',num2str(d+1),')')};
- aa(lstindx,2)={''};
- bb(lstindxOrf,1)={strcat('RF1cmp(',num2str(d+1),')')};
- bb(lstindxOrf,2)={''};
- Exp(expN).Dexp(n).RFmd1LB(d+1)=strcat(aa(lstindx,1),aa(lstindx,2));
- Exp(expN).Dexp(n).RFmd1indx(d+1)=RFmd1pos;
- Exp(expN).Dexp(n).RFmd1pltN=RF1mp;
- Exp(expN).Dexp(n).RFmd1val(d+1)=mval1;
- Exp(expN).Dexp(n).mean1(d+1)=mean1;
- Exp(expN).Dexp(n).std1(d+1)=std1;
- Exp(expN).Dexp(n).min1(d+1)=min1;
- Exp(expN).Dexp(n).max1(d+1)=max1;
- Exp(expN).Dexp(n).RFcmpL(1).dm(d+1).Lvals=medValLst; %17_1201 TrendOL
- Exp(expN).Dexp(n).RFcmpL(1).dm(d+1).med=median(medValLst); %For Ref Composite 17_1009
- Exp(expN).Dexp(n).RFcmpL(1).dm(d+1).mean=mean(medValLst); %For Ref Composite 17_1009
- Exp(expN).Dexp(n).RFcmpL(1).dm(d+1).std=std(medValLst); %For Ref Composite 17_1009
- Exp(expN).Dexp(n).RFcmpL(1).dm(d+1).min=min(medValLst); %For Ref Composite 17_1009
- Exp(expN).Dexp(n).RFcmpL(1).dm(d+1).max=max(medValLst); %For Ref Composite 17_1009
- Exp(expN).Dexp(n).RFcmpK(1).dm(d+1).Kvals=kValLst;
- Exp(expN).Dexp(n).RFcmpK(1).dm(d+1).med=median(kValLst); %For Ref Composite 17_1009
- Exp(expN).Dexp(n).RFcmpK(1).dm(d+1).mean=mean(kValLst); %For Ref Composite 17_1009
- Exp(expN).Dexp(n).RFcmpK(1).dm(d+1).std=std(kValLst); %For Ref Composite 17_1009
- Exp(expN).Dexp(n).RFcmpK(1).dm(d+1).min=min(kValLst); %For Ref Composite 17_1009
- Exp(expN).Dexp(n).RFcmpK(1).dm(d+1).max=max(kValLst); %For Ref Composite 17_1009
- Exp(expN).Dexp(n).RFcmpr(1).dm(d+1).rvals=rValLst;
- Exp(expN).Dexp(n).RFcmpr(1).dm(d+1).med=median(rValLst); %For Ref Composite 17_1009
- Exp(expN).Dexp(n).RFcmpr(1).dm(d+1).mean=mean(rValLst); %For Ref Composite 17_1009
- Exp(expN).Dexp(n).RFcmpr(1).dm(d+1).std=std(rValLst); %For Ref Composite 17_1009
- Exp(expN).Dexp(n).RFcmpr(1).dm(d+1).min=min(rValLst); %For Ref Composite 17_1009
- Exp(expN).Dexp(n).RFcmpr(1).dm(d+1).max=max(rValLst); %For Ref Composite 17_1009
- else
- % Need to set L r K values to zeros or pick the first spot with zeros
- % Spot 1 could be other than zero as the median isn't calculated if
- % fewer than some percent are nonzero
- medianCalcFailed='To Few nonZero spots for valid median RF1 selection'
- firstZero=find(((Exp(expN).Dexp(n).scan(rf1scN).plate(1).CFout(1:384,5))==0),1,'first')
- Exp(expN).Dexp(n).RFmd1indx(d+1)=firstZero;
- Exp(expN).Dexp(n).RFmd1pltN=RF1mp;
- try medValList1{d+1}=Exp(expN).Dexp(n).scan(rf1scN).plate(1).CFout(medianIndx,5); nonZeroCnt; catch, catcherror='Ln356', end
- end
- catch
- medianCalcTryFailed1='Failed! Failed! Ln362 But process continued with placeholder P1ind384 spot value!'
- try
- firstZero=find(((Exp(expN).Dexp(n).scan(rf1scN).plate(1).CFout(1:384,5))==0),1,'first')
- Exp(expN).Dexp(n).RFmd1indx(d+1)=firstZero;
- catch
- Exp(expN).Dexp(n).RFmd1indx(d+1)=384; %if crapout,Then use spot384 as default to keep going
- end
- try
- Exp(expN).Dexp(n).RFmd1pltN=RF1mp;
- catch
- Exp(expN).Dexp(n).RFmd1pltN=1;
- end
- end
- end
- end
- % Calc RF2 median if a full plate of RF2s exist
- Exp(expN).Dexp(n).RFmd2indx=[];
- Exp(expN).Dexp(n).RFmd2pltN=[];
- if exist('RF2scanN','var')
- for d=0:(DMnum-1)
- medianIndx2=[];
- nonZeroIndx2=[];
- medValList2=[];
- rf2scN=RF2scanN+d;
-
- try %if RF2scanN doesn't exist this addition to list will be skipped
- nonZeroIndx2=find(Exp(expN).Dexp(n).scan(rf2scN).plate(1).CFout(1:384,5));
- if isempty(nonZeroIndx2)
- nonZeroCnt2=0;
- medianIndx2=nonZeroIndx2;
- else
- nonZeroCnt2=length(nonZeroIndx2);
- end
- if ~isempty(nonZeroIndx2) && mod(nonZeroCnt2,2)==0, medianIndx2=nonZeroIndx2(1:nonZeroCnt2-1); end
- if ~isempty(nonZeroIndx2) && mod(nonZeroCnt2,2)~=0, medianIndx2=nonZeroIndx2; end
- clear medValLst2
- clear kValLst2
- clear rValLst2
- if ~isempty(nonZeroIndx2) && length(medianIndx2) > (.15*384) % to calc. a median, more than 15% of spots must be nonZero
- medValLst2=Exp(expN).Dexp(n).scan(rf2scN).plate(1).CFout(medianIndx2,5);
- medValList2{d+1}=medValLst2;
- min2=min(medValLst2);
- max2=max(medValLst2);
- std2=std(medValLst2);
- mean2=mean(medValLst2);
- kValLst2=Exp(expN).Dexp(n).scan(rf2scN).plate(1).CFout(medianIndx2,3); %For Ref Composite 17_1009
- rValLst2=Exp(expN).Dexp(n).scan(rf2scN).plate(1).CFout(medianIndx2,4); %For Ref Composite 17_1009
- mval2=median(medValLst2); %mval2=median(Exp(expN).Dexp(n).scan(RF2mps).plate(1).CFout(1:383,5));
- RFmd2pos=find(mval2==(Exp(expN).Dexp(n).scan(rf2scN).plate(1).CFout(1:384,5)));
- RFmd2loc(1)=ceil(RFmd2pos/24); %calc r value
- RFmd2loc(2)=RFmd2pos - ((RFmd2loc(1)-1)*24); %calc c value
- lstindx=lstindx+1;
- lstindxOrf=lstindxOrf+1;
- aa(lstindx,1)={strcat('RF2md(',num2str(d+1),')')}; %{'RF2md'};
- aa(lstindx,2)={strcat(':',num2str(RF2mp),':',num2str(RFmd2loc(1)),':',num2str(RFmd2loc(2)))}; %,'std_',num2str(std2))};
- bb(lstindxOrf,1)={strcat('RF2md(',num2str(d+1),')')}; %remove string2cell brackets {...} %{'RF2md'};
- bb(lstindxOrf,2)={strcat(':',num2str(RF2mp),':',num2str(RFmd2loc(1)),':',num2str(RFmd2loc(2)))}; %,'std_',num2str(std2))};
- % Composite Reference Amalgum Plot (CRAP)
- lstindx=lstindx+1;
- lstindxOrf=lstindxOrf+1;
- aa(lstindx,1)={strcat('RF2cmp(',num2str(d+1),')')};
- aa(lstindx,2)={''};
- bb(lstindxOrf,1)={strcat('RF2cmp(',num2str(d+1),')')};
- bb(lstindxOrf,2)={''};
- Exp(expN).Dexp(n).RFmd2LB(d+1)=strcat(aa(lstindx,1),aa(lstindx,2));
- Exp(expN).Dexp(n).RFmd2indx(d+1)=RFmd2pos;
- Exp(expN).Dexp(n).RFmd2pltN=RF2mp;
- Exp(expN).Dexp(n).RFmd2val(d+1)=mval2;
- Exp(expN).Dexp(n).mean2(d+1)=mean2;
- Exp(expN).Dexp(n).std2(d+1)=std2;
- Exp(expN).Dexp(n).min2(d+1)=min2;
- Exp(expN).Dexp(n).max2(d+1)=max2;
- Exp(expN).Dexp(n).RFcmpL(2).dm(d+1).Lvals=medValLst2; %17_1201 TrendOL
- Exp(expN).Dexp(n).RFcmpL(2).dm(d+1).med=median(medValLst2); %For Ref Composite 17_1009
- Exp(expN).Dexp(n).RFcmpL(2).dm(d+1).mean=mean(medValLst2); %For Ref Composite 17_1009
- Exp(expN).Dexp(n).RFcmpL(2).dm(d+1).std=std(medValLst2); %For Ref Composite 17_1009
- Exp(expN).Dexp(n).RFcmpL(2).dm(d+1).min=min(medValLst2); %For Ref Composite 17_1009
- Exp(expN).Dexp(n).RFcmpL(2).dm(d+1).max=max(medValLst2); %For Ref Composite 17_1009
- Exp(expN).Dexp(n).RFcmpK(2).dm(d+1).Kvals=kValLst2;
- Exp(expN).Dexp(n).RFcmpK(2).dm(d+1).med=median(kValLst2); %For Ref Composite 17_1009
- Exp(expN).Dexp(n).RFcmpK(2).dm(d+1).mean=mean(kValLst2); %For Ref Composite 17_1009
- Exp(expN).Dexp(n).RFcmpK(2).dm(d+1).std=std(kValLst2); %For Ref Composite 17_1009
- Exp(expN).Dexp(n).RFcmpK(2).dm(d+1).min=min(kValLst2); %For Ref Composite 17_1009
- Exp(expN).Dexp(n).RFcmpK(2).dm(d+1).max=max(kValLst2); %For Ref Composite 17_1009
- Exp(expN).Dexp(n).RFcmpr(2).dm(d+1).rvals=rValLst2;
- Exp(expN).Dexp(n).RFcmpr(2).dm(d+1).med=median(rValLst2); %For Ref Composite 17_1009
- Exp(expN).Dexp(n).RFcmpr(2).dm(d+1).mean=mean(rValLst2); %For Ref Composite 17_1009
- Exp(expN).Dexp(n).RFcmpr(2).dm(d+1).std=std(rValLst2); %For Ref Composite 17_1009
- Exp(expN).Dexp(n).RFcmpr(2).dm(d+1).min=min(rValLst2); %For Ref Composite 17_1009
- Exp(expN).Dexp(n).RFcmpr(2).dm(d+1).max=max(rValLst); %For Ref Composite 17_1009
- else
- % Pick the first spot with zeros
- % Use that position to satisfy and keep process OK
- medianCalcFailed2='To Few nonZero spots for valid median RF2 selection'
- firstZero2=find(((Exp(expN).Dexp(n).scan(rf2scN).plate(1).CFout(1:384,5))==0),1,'first')
- Exp(expN).Dexp(n).RFmd2indx(d+1)=firstZero2;
- Exp(expN).Dexp(n).RFmd2pltN=RF2mp;
- try medValList2{d+1}=Exp(expN).Dexp(n).scan(rf2scN).plate(1).CFout(medianIndx2,5); nonZeroCnt2; catch, catchissue='Ln431', end
- end
- catch
- medianCalcTryFailed2='Failed! Failed! Ln436 No RF1median But process continued with placeholder P1ind384 spot value!'
- try
- firstZero2=find(((Exp(expN).Dexp(n).scan(rf2scN).plate(1).CFout(1:384,5))==0),1,'first');
- Exp(expN).Dexp(n).RFmd2indx(d+1)=firstZero2;
- catch
- catchissue='Ln442'
- Exp(expN).Dexp(n).RFmd2indx(d+1)=384; %if crapout,Then use spot384 as default to keep going
- end
- try
- Exp(expN).Dexp(n).RFmd2pltN=RF2mp;
- catch
- catchissue='Ln448'
- Exp(expN).Dexp(n).RFmd2pltN=1;
- end
- end
- clear RFcombValList
- RFcombValList=cat(1,medValList1{d+1},medValList2{d+1});
- Exp(expN).Dexp(n).RFmean(d+1)=mean(RFcombValList);
- Exp(expN).Dexp(n).RFstd(d+1)=std(RFcombValList);
- try, Exp(expN).Dexp(n).RFmin(d+1)=min(RFcombValList);
- catch, Exp(expN).Dexp(n).RFmin(d+1)=0; end
- try, Exp(expN).Dexp(n).RFmax(d+1)=max(RFcombValList);
- catch, Exp(expN).Dexp(n).RFmax(d+1)=0; end
- end
- elseif exist('RF1scanN','var')
- Exp(expN).Dexp(n).RFmean=Exp(expN).Dexp(n).mean1;
- Exp(expN).Dexp(n).RFstd=Exp(expN).Dexp(n).std1;
- Exp(expN).Dexp(n).RFmin=Exp(expN).Dexp(n).min1;
- Exp(expN).Dexp(n).RFmax=Exp(expN).Dexp(n).max1;
- end
- % Disperse reference plates
- Exp(expN).Dexp(n).RFmdPindx=[];
- Exp(expN).Dexp(n).RFmdPpltN=[];
- if exist('drf','var') % ('RF1scanN','var')
- % Det. index of change from one MP to the next
- i=2;
- clear chgIndx
- chgIndx(1)=1;
- for j=1:length(drf(:,3))
- if j>1
- if cell2mat(drf(j,3))~=cell2mat(drf((j-1),3)), chgIndx(i)=j; i=i+1; end
- end
- end
- chgIndx(length(chgIndx)+1)=length(drf)+1;
- uu=zeros(size(drf,1),4);
- uu(:,1:4)=cell2mat(drf(:,3:6));
- vv=zeros(size(drf,1),DMnum); %(length(drf),DMnum);
- dMPs=unique(cell2mat(drf(:,3)));
- %{
- for mm=1:MPnum
- nonZrfIndx.mp{mm,:}=drfPindx((find(drfPindx(:,mm))),mm);
- end
- %}
- % nzValsG=zeros(DMnum,size(drf,1));
- % nzValsAcc=zeros(DMnum,384);
- for mm=1:length(dMPs) % length(chgIndx)
- for d=1:(DMnum)
- medianIndxDisp=[];
- NZrefIndxP=[];
- nonZeroValsP=[];
- medValLstDisp=[];
- rf1scNdisp=((dMPs(mm)-1)*DMnum)+(d);
- % Determine the dispersed RFs median mean and std if such exist
- try
- NZrefIndxP=drfPindx((find(drfPindx(:,dMPs(mm)))),dMPs(mm)); % find(Exp(expN).Dexp(n).scan(rf1scNdisp).plate(1).CFout(drf(chgIndx(cI):chgIndx(cI+1)),5));
- % vv=Exp(expN).Dexp(n).scan(rf1scNdisp).plate(1).CFout(NZrefIndxP,5);
- vv(chgIndx(mm):(chgIndx(mm+1)-1),d)=Exp(expN).Dexp(n).scan(rf1scNdisp).plate(1).CFout(NZrefIndxP,5);
- % For Global Ref Composite 17_1009
- vvK(chgIndx(mm):(chgIndx(mm+1)-1),d)=Exp(expN).Dexp(n).scan(rf1scNdisp).plate(1).CFout(NZrefIndxP,3);
- vvr(chgIndx(mm):(chgIndx(mm+1)-1),d)=Exp(expN).Dexp(n).scan(rf1scNdisp).plate(1).CFout(NZrefIndxP,4);
- nonZeroValsP=nonzeros(Exp(expN).Dexp(n).scan(rf1scNdisp).plate(1).CFout(NZrefIndxP,5));
- std1Disp=std(nonZeroValsP);
- mean1Disp=mean(nonZeroValsP);
- minDisp=min(nonZeroValsP);
- maxDisp=max(nonZeroValsP);
- drfVals=Exp(expN).Dexp(n).scan(rf1scNdisp).plate(1).CFout(NZrefIndxP,5);
- % nzValsAcc(mm).DM(d,:)=nonZeroValsP;
- % MEDIAN Determination for PLATES with Dispersed RFs
- % NZrefIndxP=NZrefIndxP %find(Exp(expN).Dexp(n).scan(rf1scNdisp).plate(1).CFout(NZrefIndxP,5));
- if isempty(NZrefIndxP)
- nonZeroCntDisp=0;
- medianIndxDisp=NZrefIndxP;
- else
- nonZeroCntDisp=length(NZrefIndxP);
- end
-
- if ~isempty(NZrefIndxP) && mod(nonZeroCntDisp,2)==0, medianIndxDisp=NZrefIndxP(1:nonZeroCntDisp-1); end
- if ~isempty(NZrefIndxP) && mod(nonZeroCntDisp,2)~=0, medianIndxDisp=NZrefIndxP; end
- if ~isempty(NZrefIndxP) && length(medianIndxDisp) > 0 %(.15*384) %To calc. a median, more than 15% of spots must be nonZero
- medValLstDisp=Exp(expN).Dexp(n).scan(rf1scNdisp).plate(1).CFout(medianIndxDisp,5);
- %medValList1Disp{d}=medValLstDisp;
- mval1Disp=median(medValLstDisp); % mval1=median(Exp(expN).Dexp(n).scan(RF1mps).plate(1).CFout(1:384,5)); %CFout(1:383,5));
- nonZeroMedPosIndx=find(mval1Disp==nonZeroValsP); % (Exp(expN).Dexp(n).scan(rf1scNdisp).plate(1).CFout(NZrefIndxP,5))); %(nonZeroIndxDisp,5))); %((nonZrfIndx.mp{dMPs(mm),:}),5)));
- RFmd1posD=NZrefIndxP(nonZeroMedPosIndx); % drfPindx((RFmd1posIndx),dMPs(mm));
- RFmd1locD(1)=ceil(RFmd1posD/24); % calc r value
- RFmd1locD(2)=RFmd1posD - ((RFmd1locD(1)-1)*24); % calc c value
- lstindx=lstindx+1;
- lstindxOrf=lstindxOrf+1;
- if usrRefFg==0, rfPLbl='RF1mdP('; else rfPLbl=strcat('RF-',rfGnSp,'mdP('); end % accomodate User Ref Selection
- if iscell(strcat(rfPLbl,num2str(d),')')),
- aa(lstindx,1)=strcat(rfPLbl,num2str(d),')');
- bb(lstindxOrf,1)=strcat(rfPLbl,num2str(d),')'); % remove string2cell brackets {...}
- else
- aa(lstindx,1)={strcat(rfPLbl,num2str(d),')')};
- bb(lstindxOrf,1)={strcat(rfPLbl,num2str(d),')')}; %remove string2cell brackets {...}
- end
- aa(lstindx,2)={strcat(':',num2str(dMPs(mm)),':',num2str(RFmd1locD(1)),':',num2str(RFmd1locD(2)))};
- bb(lstindxOrf,2)={strcat(':',num2str(dMPs(mm)),':',num2str(RFmd1locD(1)),':',num2str(RFmd1locD(2)))};
- Exp(expN).Dexp(n).RFmdPLB(d,dMPs(mm))=strcat(aa(lstindx,1),aa(lstindx,2));
- Exp(expN).Dexp(n).RFmdPindx(d,dMPs(mm))=RFmd1posD;
- Exp(expN).Dexp(n).RFmdPpltN(d,dMPs(mm))=dMPs(mm); %This is a storage of the MasterPlate used NOT the ScanPlate? WeMight wish to store the ScanPlateNumber also???
- Exp(expN).Dexp(n).RFmdPscanN(d,dMPs(mm))=rf1scNdisp;
- Exp(expN).Dexp(n).RFmedianP(d,dMPs(mm))=mval1Disp;
- Exp(expN).Dexp(n).stdP(d,dMPs(mm))=std1Disp;
- Exp(expN).Dexp(n).meanP(d,dMPs(mm))=mean1Disp;
- Exp(expN).Dexp(n).minP(d,dMPs(mm))=minDisp;
- Exp(expN).Dexp(n).maxP(d,dMPs(mm))=maxDisp;
- else
- % Need to set L r K values to zeros or pick the first spot with zeros
- % Spot 1 could be other than zero as the median isn't calculated if fewer than some percent are nonzero
- medianCalcFailedD='To Few nonZero spots for valid median RF1 selection'
- firstZeroDindx=find(((Exp(expN).Dexp(n).scan(rf1scNdisp).plate(1).CFout(NZrefIndxP,5))==0),1,'first');
- firstZeroD=drfPindx((firstZeroDindx),dMPs(mm));
- Exp(expN).Dexp(n).RFmdPindx(d,dMPs(mm))=firstZeroD;
- Exp(expN).Dexp(n).RFmdPpltN(d,dMPs(mm))=dMPs(mm); % RF1mp; % Is this supposed to be the MP number or the ScanPlate number???
- end
- catch
- medianCalcTryFailed1D='Failed! Failed! Ln565 {Dispersed RF1s} "try" But process continued with placeholder Plate spot value!'
- try
- firstZeroindxD=find(vv((chgIndx(d):chgIndx(d+1)-1),5)==0,1,'first');
- firstZeroPindx=uu(firstZeroindxD,4);
- Exp(expN).Dexp(n).RFmdPpltN(d,dMPs(mm))=dMPs(mm);
- Exp(expN).Dexp(n).RFmdPindx(d,dMPs(mm))=firstZeroPindx;
- catch
- catchissue='try at Ln565'
- Exp(expN).Dexp(n).RFmdPpltN(d,dMPs(mm))=dMPs(mm);
- Exp(expN).Dexp(n).RFmdPindx(d,dMPs(mm))=384; % if crapout,Then use spot384 as default to keep going
- end
- try
- Exp(expN).Dexp(n).RFmdPpltN=dMPs(mm);
- catch
- catchissue='Ln619'
- Exp(expN).Dexp(n).RFmdPpltN=1; % this might keep from breaking BUT ???
- end
- end
- end
- end
- % Calc. Global Mean, Std, And Median For Distributed References
- uu=horzcat(uu,vv);
- for d=1:DMnum
- medianIndxG=[];
- NZrfIndxG=[];
- minG=min(nonzeros(vv(:,d)));
- maxG=max(nonzeros(vv(:,d)));
- RFmeanG=mean(nonzeros(vv(:,d)));
- RFstdG=std(nonzeros(vv(:,d)));
- NZrfIndxG=find(vv(:,d)); % find(nonzeros(vv(:,d)));
-
- if isempty(RFmeanG)
- nonZeroCntD=0 % medianIndxDisp=RFmeanG(d);
- else
- nonZeroCntD=length(nonzeros(vv(:,d)));
- end
- % Calc. of median requires an odd number of indx items
- if ~isempty(NZrfIndxG) && mod(nonZeroCntD,2)==0, medianIndxD=NZrfIndxG(1:(size(NZrfIndxG,1)-1)); end
- if ~isempty(NZrfIndxG) && mod(nonZeroCntD,2)~=0, medianIndxD=NZrfIndxG(:); end
- if ~isempty(NZrfIndxG) && length(medianIndxD) > 0 %(.15*size(drf,1)) % to calc. a median, more than 15% of spots must be nonZero
- mvalsDG=median(vv(medianIndxD,d)); % (nonzeros(vv(:,d)));
- nzPosIndxDG=find(mvalsDG==vv(:,d));
- RFmdDGloc(d,1)=uu(nzPosIndxDG,1); % MP of distributedGlobal Median value
- RFmdDGloc(d,2)=uu(nzPosIndxDG,2); % calc r value
- RFmdDGloc(d,3)=uu(nzPosIndxDG,3); % calc c value
- lstindx=lstindx+1;
- lstindxOrf=lstindxOrf+1;
- if usrRefFg==0, rfGLbl='RF1mdG('; else rfGLbl=strcat('RF-',rfGnSp,'mdG('); end %accomodate User Ref Selection
- if iscell(strcat(rfPLbl,num2str(d),')')),
- aa(lstindx,1)=strcat(rfGLbl,num2str(d),')'); % remove string2cell brackets {...}
- bb(lstindxOrf,1)=strcat(rfGLbl,num2str(d),')'); % remove string2cell brackets {...}
- else
- aa(lstindx,1)={strcat(rfGLbl,num2str(d),')')};
- bb(lstindxOrf,1)={strcat(rfGLbl,num2str(d),')')};
- end
- aa(lstindx,2)={strcat(':',num2str(RFmdDGloc(d,1)),':',num2str(RFmdDGloc(d,2)),':',num2str(RFmdDGloc(d,3)))}; %,'std_',num2str(std2))};
- bb(lstindxOrf,2)={strcat(':',num2str(RFmdDGloc(d,1)),':',num2str(RFmdDGloc(d,2)),':',num2str(RFmdDGloc(d,3)))}; %,'std_',num2str(std2))};
- % Composite Reference Amalgum Plot
- lstindx=lstindx+1;
- lstindxOrf=lstindxOrf+1;
- if usrRefFg==0, rfcmpGLbl='RFcmpG('; else rfcmpGLbl=strcat('RFcmpG-',rfGnSp,'('); end %accomodate User Ref Selection
- if iscell(strcat(rfcmpGLbl,num2str(d),')')),
- aa(lstindx,1)=strcat(rfcmpGLbl,num2str(d),')'); % strcat('RFcmpG(',num2str(d),')');
- bb(lstindxOrf,1)=strcat(rfcmpGLbl,num2str(d),')'); % strcat('RFcmpG(',num2str(d),')');
- else
- aa(lstindx,1)={strcat(rfcmpGLbl,num2str(d),')')};
- bb(lstindxOrf,1)={strcat(rfcmpGLbl,num2str(d),')')};
- end
- aa(lstindx,2)={''};
- bb(lstindxOrf,2)={''};
- Exp(expN).Dexp(n).RFmdGLB(d)=strcat(aa(lstindx,1),aa(lstindx,2));
- Exp(expN).Dexp(n).RFmdGindx(d)=nzPosIndxDG;
- Exp(expN).Dexp(n).RFmdGpltN(d)=RFmdDGloc(d,1);
- Exp(expN).Dexp(n).RFmdGscanN(d)=uu(nzPosIndxDG,4);
- Exp(expN).Dexp(n).RFmedianG(d)=mvalsDG;
- Exp(expN).Dexp(n).RFminG(d)=minG;
- Exp(expN).Dexp(n).RFmaxG(d)=maxG;
- Exp(expN).Dexp(n).RFstdG(d)=RFstdG;
- Exp(expN).Dexp(n).RFmeanG(d)=RFmeanG;
- Exp(expN).Dexp(n).RFcmpGL.dm(d).Lvals=vv(medianIndxD,d); %17_1201 TrendOL
- Exp(expN).Dexp(n).RFcmpGL.dm(d).med=median(vv(medianIndxD,d)); %For Ref Composite 17_1009
- Exp(expN).Dexp(n).RFcmpGL.dm(d).mean=mean(vv(medianIndxD,d)); %For Ref Composite 17_1009
- Exp(expN).Dexp(n).RFcmpGL.dm(d).std=std(vv(medianIndxD,d)); %For Ref Composite 17_1009
- Exp(expN).Dexp(n).RFcmpGL.dm(d).min=min(vv(medianIndxD,d)); %For Ref Composite 17_1009
- Exp(expN).Dexp(n).RFcmpGL.dm(d).max=max(vv(medianIndxD,d)); %For Ref Composite 17_1009
- Exp(expN).Dexp(n).RFcmpGK.dm(d).Kvals=vvK(medianIndxD,d); %17_1201 TrendOL
- Exp(expN).Dexp(n).RFcmpGK.dm(d).med=median(vvK(medianIndxD,d)); %For Ref Composite 17_1009
- Exp(expN).Dexp(n).RFcmpGK.dm(d).mean=mean(vvK(medianIndxD,d)); %For Ref Composite 17_1009
- Exp(expN).Dexp(n).RFcmpGK.dm(d).std=std(vvK(medianIndxD,d)); %For Ref Composite 17_1009
- Exp(expN).Dexp(n).RFcmpGK.dm(d).min=min(vvK(medianIndxD,d)); %For Ref Composite 17_1009
- Exp(expN).Dexp(n).RFcmpGK.dm(d).max=max(vvK(medianIndxD,d)); %For Ref Composite 17_1009
- Exp(expN).Dexp(n).RFcmpGr.dm(d).rvals=vvr(medianIndxD,d); %17_1201 TrendOL
- Exp(expN).Dexp(n).RFcmpGr.dm(d).med=median(vvr(medianIndxD,d)); %For Ref Composite 17_1009
- Exp(expN).Dexp(n).RFcmpGr.dm(d).mean=mean(vvr(medianIndxD,d)); %For Ref Composite 17_1009
- Exp(expN).Dexp(n).RFcmpGr.dm(d).std=std(vvr(medianIndxD,d)); %For Ref Composite 17_1009
- Exp(expN).Dexp(n).RFcmpGr.dm(d).min=min(vvr(medianIndxD,d)); %For Ref Composite 17_1009
- Exp(expN).Dexp(n).RFcmpGr.dm(d).max=max(vvr(medianIndxD,d)); %For Ref Composite 17_1009
- end
- end
- end
- % EZusrSelRef
- gnLstRaw=strcat(aa(:,1),aa(:,2));
- Exp(expN).Dexp(n).srtGnLst=sort(gnLstRaw);
- orfLstRaw=strcat(bb(:,1),bb(:,2));
- Exp(expN).Dexp(n).srtOrfLst=sort(orfLstRaw);
- %{
- %set(handles.MPsldr2,'max',length(MP));
- %set(handles.DMsldr2,'max',length(DM.drug));
- %set(handles.Tptsldr2,'max',tPtsSize);
- %set(handles.MPsldr2,'min',1,'max',MPnum)
- %set(handles.DMsldr2,'min',1,'max',DMnum)
- %tPtsSize=size(Exp(expN).Dexp(n).FexpScanBMtp{1,1},(3));
- %set(handles.Tptsldr2,'min',1,'max',tPtsSize)
- %set(handles.MPsldr2,'value',1)
- %set(handles.DMsldr2,'value',1)
- %set(handles.Tptsldr2,'value',1)
- %set(handles.MPed2,'string','1')
- %set(handles.DMed2,'string','1')
- %set(handles.Tpted2,'string','1')
- %set(handles.MPsldr2,'SliderStep',[1/length(MP) 1/length(MP)]);
- %set(handles.DMsldr2,'SliderStep',[1/length(DM.drug) 1/length(DM.drug)]);
- %set(handles.Tptsldr2,'SliderStep',[1/tPtsSize 1/tPtsSize]);
- %}
|